RLG00000008747

3beta-hydroxysteroid-dehydrogenase decarboxylase isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003789
Physical Location & Seq
Reverse (-)
36227099 .. 36231714
4616 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008747

Sequence Viewer

Length: 1641 bp
ATGACCTGTGATGAGAGATGGTGCGTTGTCACCGGAGGCCGAGGCTTCGCTGCTCGCCATTTGGTTCTTGAACCTTCCGAGTCCAACGGAGTCCTCGCTGATGCTTTGAACTTCAGCCGCGCTGAGTACATTTCCATTGACCTCCGCTGCAAGGATCAAGTTCTTCAAGCATGTCAAGGTGCTGAGGTTGTGTTCCATATGGCGGCGCCTAATTCGTCGATCAACAATTACCAGCTCCACCATTCGGTCAATGTGGAAGGAACTAAAAATGTGATTGATGCTTGTGTTAAGCAGAAAGTGAAGAGGCTCATATACACTAGCTCTCCTAGCGTTGTGTTTGATGGGGTTCATGGTATTATTGATGGGGATGAGTCGTTGTCATATCCACCTAAGCACAATGATACTTATTCGGCCACTAAAGCACAAGGAGAAGAGTTGGTAATCAAGGCAAACGGTGTTAAAGGGCTTCTAACCTGCTGCATACGCCCTAGCAGCATTTTCGGACCCGGTGATAGGTTACTGGTTCCGTCTTTAGTTGTTGCCGCCAGGGCAGGGAAATCCAAGTTCATTATAGGAGATGGCAATAACATTTATGATTTCACGTATGTTGAAAACGTGGCCCATGCCCATATATGTGCAGAGCGAGCTCTAGCATTTGAAGGCACAGTTGCAGAGAAAGCTGCAGGACAGGCATATTTTATAACCAATATGGAACCTATTAAGTTTTGGGAGTTTATTTCACTTATTCTAGAAGGTCTTGGTTATGAAAGACCAAGGATAAAAATCCCTACAGCCATTATGATGCCAATTGCACATGTGGTGGAATGGACACATAAGCTTTTAGGCCCGTATGGGATGAAGGTTCCACAGTTGACGCCTTCAAGAATTAGACTTCTCTCTTGCACCAGATCTTTTAAATCTTCAAAAGCACAGGATCGAATTGGTTACACACCCATTATTTCGCTTCAGGAGGGTCTTAATAGGACAATTGAGTCGCACCCACACTTGAGTGCTGAACATCGACCCAAAAGAGAAGGGCCATCTAAAGCTTCTGTATATCTTGGAAGCGGAAGGGTTGCTGACGCATTACTGTGGAAGGATAAAAAACAGACATTCAAGGCACTATTAGTTTTGATTGCGATCTACTACAACTTCATTGAATCTAAAGCTACGATTATTACTGTCATTTCAAAGAGTCTCATGTTCGCATCAATTTTCGTGTTCATCTCTGGCAATTTACCAGAAATGATATTAGGATATAAACTCAAGAAGCTTCAATCATCTCATTTTCACTTCTCGGAAGAGATGTCACAACAAATTGCTGCATCTGTGGTCTCATCATGGAATATATCTGCCAAAACTTTAAAATCTCTCGGGAAAGGAAATGACTGCATATTGTTTCTTAAGGCGGCTGTATCTCTACTGGTTCTTAGCTTTCTTGGAGCTATTTCACTTCAGAACTCATTTATTATAGGTTATTCTCTCTCCTTCCTAATGAGTTCCACTGCCTTTTTAGCCTTCTATGTGTATGAAAAGCAGGAAGAAAAAATCGATGGCATGGTTCTAGAAGCTTTCTCTCGTGGACGCAAATTGAAGTCCAATATACTTCGAAAATTCAGTACATCCAAAAGGATTGAGTGA

Protein Analysis

547

Amino Acids

60.4

Weight (kDa)

9.2

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 9 - 214 6.8e-32 NAD dependent epimerase/dehydratase family
RmlD_sub_bind PF04321 9 - 261 1.8e-08 RmlD substrate binding domain
3Beta_HSD PF01073 26 - 262 1.5e-69 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 39 - 112 1.7e-07 NAD(P)H-binding
GDP_Man_Dehyd PF16363 44 - 211 2.4e-09 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 56 - 208 5.6e-14 Male sterility protein
Reticulon PF02453 359 - 521 5.2e-26 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 701
AasI GACNNNNNNGTC 1 cut(s) 991
Acc36I ACCTGC 1 cut(s) 482
AccB1I GGYRCC 1 cut(s) 205
AccII CGCG 1 cut(s) 120
AciI CCGC 6 cut(s) 118, 145, 203, 543, 1068, 1409
AclWI GGATC 2 cut(s) 162, 942
AcoI YGGCCR 1 cut(s) 411
AcsI RAATTY 1 cut(s) 1613
AcuI CTGAAG 3 cut(s) 97, 950, 1439
AcyI GRCGYC 2 cut(s) 206, 875
AfaI GTAC 2 cut(s) 128, 1621
AfiI CCNNNNNNNGG 5 cut(s) 151, 202, 244, 513, 552
AflII CTTAAG 1 cut(s) 1403
AflIII ACRYGT 1 cut(s) 814
AjnI CCWGG 1 cut(s) 545
AleI CACNNNNGTG 1 cut(s) 1008
Alw21I GWGCWC 1 cut(s) 649
Alw26I GTCTC 2 cut(s) 1202, 1339
AlwI GGATC 2 cut(s) 162, 942
Ama87I CYCGRG 1 cut(s) 1373
AoxI GGCC 5 cut(s) 37, 411, 618, 844, 1037
ApeKI GCWGC 6 cut(s) 50, 147, 477, 492, 680, 1322
ApoI RAATTY 1 cut(s) 1613
AspLEI GCGC 2 cut(s) 122, 208
AspS9I GGNCC 4 cut(s) 503, 619, 845, 1037
AsuC2I CCSGG 1 cut(s) 507
AsuHPI GGTGA 2 cut(s) 22, 521
AsuII TTCGAA 1 cut(s) 1609
AvaI CYCGRG 1 cut(s) 1373
AvaII GGWCC 1 cut(s) 503
BanI GGYRCC 1 cut(s) 205
BanII GRGCYC 1 cut(s) 649
BauI CACGAG 1 cut(s) 1578
Bbv12I GWGCWC 1 cut(s) 649
BbvCI CCTCAGC 1 cut(s) 183
BbvI GCAGC 6 cut(s) 37, 134, 464, 504, 667, 1309
BccI CCATC 6 cut(s) 12, 335, 356, 572, 1048, 1547
BcgI CGANNNNNNTGC 2 cut(s) 481, 515
BciT130I CCWGG 1 cut(s) 547
BcnI CCSGG 1 cut(s) 507
BcoDI GTCTC 2 cut(s) 1202, 1339
BfaI CTAG 6 cut(s) 318, 327, 489, 650, 749, 1565
BfmI CTRYAG 2 cut(s) 681, 789
BfoI RGCGCY 1 cut(s) 209
BfrI CTTAAG 1 cut(s) 1403
BfuAI ACCTGC 1 cut(s) 482
BglI GCCNNNNNGGC 1 cut(s) 548
BglII AGATCT 1 cut(s) 908
Bme1390I CCNGG 2 cut(s) 507, 547
Bme18I GGWCC 1 cut(s) 503
BmeT110I CYCGRG 1 cut(s) 1373
BmgT120I GGNCC 4 cut(s) 503, 619, 845, 1037
BmiI GGNNCC 5 cut(s) 207, 505, 525, 714, 864
BmrFI CCNGG 2 cut(s) 507, 547
BmsI GCATC 5 cut(s) 91, 268, 792, 1217, 1334
Bpu10I CCTNAGC 2 cut(s) 183, 390
Bpu14I TTCGAA 1 cut(s) 1609
BpuEI CTTGAG 2 cut(s) 1027, 1250
BpuMI CCSGG 1 cut(s) 507
Bsa29I ATCGAT 1 cut(s) 1551
BsaAI YACGTR 1 cut(s) 603
BsaBI GATNNNNATC 2 cut(s) 782, 1139
BsaHI GRCGYC 2 cut(s) 206, 875
BsaI GGTCTC 1 cut(s) 1339
BsaJI CCNNGG 3 cut(s) 40, 546, 773
BsaWI WCCGGW 1 cut(s) 32
BsaXI ACNNNNNCTCC 2 cut(s) 307, 337
Bsc4I CCNNNNNNNGG 5 cut(s) 151, 202, 244, 513, 552
Bse1I ACTGG 2 cut(s) 525, 1428
Bse8I GATNNNNATC 2 cut(s) 782, 1139
BseBI CCWGG 1 cut(s) 547
BseCI ATCGAT 1 cut(s) 1551
BseDI CCNNGG 3 cut(s) 40, 546, 773
BseGI GGATG 3 cut(s) 373, 861, 1622
BseJI GATNNNNATC 2 cut(s) 782, 1139
BseLI CCNNNNNNNGG 5 cut(s) 151, 202, 244, 513, 552
BseMII CTCAG 2 cut(s) 114, 174
BseNI ACTGG 2 cut(s) 525, 1428
BseXI GCAGC 6 cut(s) 37, 134, 464, 504, 667, 1309
BsgI GTGCAG 1 cut(s) 657
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 5 cut(s) 39, 413, 620, 846, 1039
BshNI GGYRCC 1 cut(s) 205
BshVI ATCGAT 1 cut(s) 1551
BsiHKAI GWGCWC 1 cut(s) 649
BsiHKCI CYCGRG 1 cut(s) 1373
BsiSI CCGG 2 cut(s) 33, 507
BslI CCNNNNNNNGG 5 cut(s) 151, 202, 244, 513, 552
BsmAI GTCTC 2 cut(s) 1202, 1339
BsnI GGCC 5 cut(s) 39, 413, 620, 846, 1039
Bso31I GGTCTC 1 cut(s) 1339
BsoBI CYCGRG 1 cut(s) 1373
Bsp119I TTCGAA 1 cut(s) 1609
Bsp1286I GDGCHC 1 cut(s) 649
Bsp143I GATC 5 cut(s) 154, 219, 908, 934, 1140
BspACI CCGC 6 cut(s) 118, 145, 203, 543, 1068, 1409
BspANI GGCC 5 cut(s) 39, 413, 620, 846, 1039
BspCNI CTCAG 2 cut(s) 115, 175
BspDI ATCGAT 1 cut(s) 1551
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 5 cut(s) 207, 505, 525, 714, 864
BspMAI CTGCAG 1 cut(s) 685
BspMI ACCTGC 1 cut(s) 482
BspPI GGATC 2 cut(s) 162, 942
BspT104I TTCGAA 1 cut(s) 1609
BspT107I GGYRCC 1 cut(s) 205
BspTI CTTAAG 1 cut(s) 1403
BspTNI GGTCTC 1 cut(s) 1339
BsrI ACTGG 2 cut(s) 525, 1428
BssECI CCNNGG 3 cut(s) 40, 546, 773
BssMI GATC 5 cut(s) 154, 219, 908, 934, 1140
BssNI GRCGYC 2 cut(s) 206, 875
BssSI CACGAG 1 cut(s) 1578
BssT1I CCWWGG 1 cut(s) 773
Bst2BI CACGAG 1 cut(s) 1578
Bst2UI CCWGG 1 cut(s) 547
Bst4CI ACNGT 5 cut(s) 455, 667, 870, 1092, 1183
Bst6I CTCTTC 3 cut(s) 296, 426, 1296
BstACI GRCGYC 2 cut(s) 206, 875
BstAFI CTTAAG 1 cut(s) 1403
BstBAI YACGTR 1 cut(s) 603
BstBI TTCGAA 1 cut(s) 1609
BstC8I GCNNGC 2 cut(s) 55, 645
BstDEI CTNAG 4 cut(s) 123, 183, 390, 1430
BstF5I GGATG 3 cut(s) 373, 861, 1622
BstFNI CGCG 1 cut(s) 120
BstH2I RGCGCY 1 cut(s) 209
BstHHI GCGC 2 cut(s) 122, 208
BstKTI GATC 5 cut(s) 157, 222, 911, 937, 1143
BstMAI GTCTC 2 cut(s) 1202, 1339
BstMBI GATC 5 cut(s) 154, 219, 908, 934, 1140
BstMWI GCNNNNNNNGC 9 cut(s) 327, 419, 483, 492, 548, 644, 677, 689, 1514
BstNI CCWGG 1 cut(s) 547
BstNSI RCATGY 2 cut(s) 174, 818
BstSCI CCNGG 2 cut(s) 505, 545
BstSFI CTRYAG 2 cut(s) 681, 789
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 6 cut(s) 37, 134, 464, 504, 667, 1309
BstX2I RGATCY 1 cut(s) 908
BstYI RGATCY 1 cut(s) 908
Bsu15I ATCGAT 1 cut(s) 1551
BsuRI GGCC 5 cut(s) 39, 413, 620, 846, 1039
BsuTUI ATCGAT 1 cut(s) 1551
BtsCI GGATG 3 cut(s) 373, 861, 1622
BtsI GCAGTG 1 cut(s) 1503
BtsIMutI CAGTG 1 cut(s) 1503
BveI ACCTGC 1 cut(s) 482
Cac8I GCNNGC 2 cut(s) 55, 645
CfoI GCGC 2 cut(s) 122, 208
Cfr13I GGNCC 4 cut(s) 503, 619, 845, 1037
ClaI ATCGAT 1 cut(s) 1551
CseI GACGC 3 cut(s) 883, 1091, 1593
Csp6I GTAC 2 cut(s) 127, 1620
CviAII CATG 7 cut(s) 171, 350, 623, 815, 1201, 1341, 1558
CviQI GTAC 2 cut(s) 127, 1620
DdeI CTNAG 4 cut(s) 123, 183, 390, 1430
DinI GGCGCC 1 cut(s) 207
DpnI GATC 5 cut(s) 156, 221, 910, 936, 1142
DpnII GATC 5 cut(s) 154, 219, 908, 934, 1140
DraI TTTAAA 2 cut(s) 916, 1365
DrdI GACNNNNNNGTC 1 cut(s) 991
DseDI GACNNNNNNGTC 1 cut(s) 991
EaeI YGGCCR 1 cut(s) 411
Eam1104I CTCTTC 3 cut(s) 296, 426, 1296
EarI CTCTTC 3 cut(s) 296, 426, 1296
Ecl136II GAGCTC 1 cut(s) 647
Eco130I CCWWGG 1 cut(s) 773
Eco24I GRGCYC 1 cut(s) 649
Eco31I GGTCTC 1 cut(s) 1339
Eco47I GGWCC 1 cut(s) 503
Eco53kI GAGCTC 1 cut(s) 647
Eco57I CTGAAG 3 cut(s) 97, 950, 1439
Eco88I CYCGRG 1 cut(s) 1373
EcoICRI GAGCTC 1 cut(s) 647
EcoRII CCWGG 1 cut(s) 545
EcoT14I CCWWGG 1 cut(s) 773
EcoT38I GRGCYC 1 cut(s) 649
EgeI GGCGCC 1 cut(s) 207
EheI GGCGCC 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 773
FaeI CATG 7 cut(s) 174, 353, 626, 818, 1204, 1344, 1561
FatI CATG 7 cut(s) 170, 349, 622, 814, 1200, 1340, 1557
FauNDI CATATG 1 cut(s) 198
FokI GGATG 3 cut(s) 380, 868, 1609
FriOI GRGCYC 1 cut(s) 649
FspBI CTAG 6 cut(s) 318, 327, 489, 650, 749, 1565
GlaI GCGC 2 cut(s) 121, 207
HaeII RGCGCY 1 cut(s) 209
HaeIII GGCC 5 cut(s) 39, 413, 620, 846, 1039
HapII CCGG 2 cut(s) 33, 507
HgaI GACGC 3 cut(s) 883, 1091, 1593
HhaI GCGC 2 cut(s) 122, 208
Hin1I GRCGYC 2 cut(s) 206, 875
Hin1II CATG 7 cut(s) 174, 353, 626, 818, 1204, 1344, 1561
Hin6I GCGC 2 cut(s) 120, 206
HinP1I GCGC 2 cut(s) 120, 206
HincII GTYRAC 1 cut(s) 873
HindII GTYRAC 1 cut(s) 873
HindIII AAGCTT 4 cut(s) 836, 1047, 1271, 1569
HinfI GANTC 6 cut(s) 80, 90, 371, 992, 1160, 1195
HpaII CCGG 2 cut(s) 33, 507
HphI GGTGA 2 cut(s) 22, 521
Hpy166II GTNNAC 2 cut(s) 873, 1583
Hpy188I TCNGA 4 cut(s) 79, 503, 1300, 1458
Hpy188III TCNNGA 7 cut(s) 68, 749, 882, 968, 1267, 1375, 1565
Hpy8I GTNNAC 2 cut(s) 873, 1583
Hpy99I CGWCG 1 cut(s) 220
HpyCH4III ACNGT 5 cut(s) 455, 667, 870, 1092, 1183
HpyCH4IV ACGT 2 cut(s) 602, 615
HpyCH4V TGCA 9 cut(s) 150, 480, 638, 671, 683, 812, 903, 1325, 1392
HpyF10VI GCNNNNNNNGC 9 cut(s) 327, 419, 483, 492, 548, 644, 677, 689, 1514
HpyF3I CTNAG 4 cut(s) 123, 183, 390, 1430
HpySE526I ACGT 2 cut(s) 602, 615
Hsp92I GRCGYC 2 cut(s) 206, 875
Hsp92II CATG 7 cut(s) 174, 353, 626, 818, 1204, 1344, 1561
HspAI GCGC 2 cut(s) 120, 206
KasI GGCGCC 1 cut(s) 205
Kzo9I GATC 5 cut(s) 154, 219, 908, 934, 1140
LmnI GCTCC 2 cut(s) 240, 1442
Lsp1109I GCAGC 6 cut(s) 37, 134, 464, 504, 667, 1309
LweI GCATC 5 cut(s) 91, 268, 792, 1217, 1334
MaeI CTAG 6 cut(s) 318, 327, 489, 650, 749, 1565
MaeII ACGT 2 cut(s) 602, 615
MaeIII GTNAC 4 cut(s) 28, 516, 944, 1308
MalI GATC 5 cut(s) 156, 221, 910, 936, 1142
MboI GATC 5 cut(s) 154, 219, 908, 934, 1140
MboII GAAGA 6 cut(s) 155, 313, 443, 912, 1313, 1553
MfeI CAATTG 2 cut(s) 807, 987
MflI RGATCY 1 cut(s) 908
MhlI GDGCHC 1 cut(s) 649
Mly113I GGCGCC 1 cut(s) 206
MlyI GAGTC 5 cut(s) 89, 99, 380, 1001, 1204
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 7 cut(s) 29, 35, 104, 152, 178, 297, 964
MseI TTAA 7 cut(s) 288, 459, 720, 915, 978, 1364, 1404
MslI CAYNNNNRTG 4 cut(s) 633, 800, 1008, 1090
MspA1I CMGCKG 1 cut(s) 147
MspCI CTTAAG 1 cut(s) 1403
MspI CCGG 2 cut(s) 33, 507
MspR9I CCNGG 2 cut(s) 507, 547
MunI CAATTG 2 cut(s) 807, 987
MvaI CCWGG 1 cut(s) 547
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 9 cut(s) 327, 419, 483, 492, 548, 644, 677, 689, 1514
NarI GGCGCC 1 cut(s) 206
NciI CCSGG 1 cut(s) 507
NdeI CATATG 1 cut(s) 198
NdeII GATC 5 cut(s) 154, 219, 908, 934, 1140
NlaIII CATG 7 cut(s) 174, 353, 626, 818, 1204, 1344, 1561
NlaIV GGNNCC 5 cut(s) 207, 505, 525, 714, 864
NmeAIII GCCGAG 1 cut(s) 65
NmuCI GTSAC 2 cut(s) 28, 1308
NspI RCATGY 2 cut(s) 174, 818
NspV TTCGAA 1 cut(s) 1609
OliI CACNNNNGTG 1 cut(s) 1008
PciI ACATGT 1 cut(s) 814
PfeI GAWTC 1 cut(s) 1160
PleI GAGTC 5 cut(s) 88, 98, 379, 1000, 1203
PluTI GGCGCC 1 cut(s) 209
PpsI GAGTC 5 cut(s) 88, 98, 379, 1000, 1203
Ppu21I YACGTR 1 cut(s) 603
PscI ACATGT 1 cut(s) 814
PsiI TTATAA 1 cut(s) 701
Psp124BI GAGCTC 1 cut(s) 649
Psp6I CCWGG 1 cut(s) 545
PspGI CCWGG 1 cut(s) 545
PspN4I GGNNCC 5 cut(s) 207, 505, 525, 714, 864
PspPI GGNCC 4 cut(s) 503, 619, 845, 1037
PstI CTGCAG 1 cut(s) 685
PsuI RGATCY 1 cut(s) 908
RsaI GTAC 2 cut(s) 128, 1621
RsaNI GTAC 2 cut(s) 127, 1620
RseI CAYNNNNRTG 4 cut(s) 633, 800, 1008, 1090
SacI GAGCTC 1 cut(s) 649
SaqAI TTAA 7 cut(s) 288, 459, 720, 915, 978, 1364, 1404
Sau3AI GATC 5 cut(s) 154, 219, 908, 934, 1140
Sau96I GGNCC 4 cut(s) 503, 619, 845, 1037
SchI GAGTC 5 cut(s) 89, 99, 380, 1001, 1204
ScrFI CCNGG 2 cut(s) 507, 547
SduI GDGCHC 1 cut(s) 649
SfaNI GCATC 5 cut(s) 91, 268, 792, 1217, 1334
SfcI CTRYAG 2 cut(s) 681, 789
SfoI GGCGCC 1 cut(s) 207
SfuI TTCGAA 1 cut(s) 1609
SinI GGWCC 1 cut(s) 503
SmiMI CAYNNNNRTG 4 cut(s) 633, 800, 1008, 1090
SmlI CTYRAG 3 cut(s) 1006, 1265, 1403
SmoI CTYRAG 3 cut(s) 1006, 1265, 1403
SsiI CCGC 6 cut(s) 118, 145, 203, 543, 1068, 1409
SspDI GGCGCC 1 cut(s) 205
SspMI CTAG 6 cut(s) 318, 327, 489, 650, 749, 1565
SstI GAGCTC 1 cut(s) 649
StyD4I CCNGG 2 cut(s) 505, 545
StyI CCWWGG 1 cut(s) 773
TaaI ACNGT 5 cut(s) 455, 667, 870, 1092, 1183
TaiI ACGT 2 cut(s) 605, 618
TaqI TCGA 5 cut(s) 218, 937, 1021, 1551, 1609
TaqII GACCGA 1 cut(s) 235
TatI WGTACW 2 cut(s) 126, 1619
TauI GCSGC 4 cut(s) 120, 206, 545, 1412
TfiI GAWTC 1 cut(s) 1160
Tru1I TTAA 7 cut(s) 288, 459, 720, 915, 978, 1364, 1404
Tru9I TTAA 7 cut(s) 288, 459, 720, 915, 978, 1364, 1404
TscAI CASTG 1 cut(s) 1510
TseFI GTSAC 2 cut(s) 28, 1308
TseI GCWGC 6 cut(s) 50, 147, 477, 492, 680, 1322
Tsp45I GTSAC 2 cut(s) 28, 1308
TspDTI ATGAA 7 cut(s) 338, 556, 780, 872, 1144, 1213, 1545
TspGWI ACGGA 2 cut(s) 102, 516
TspRI CASTG 1 cut(s) 1510
Vha464I CTTAAG 1 cut(s) 1403
VpaK11BI GGWCC 1 cut(s) 503
XapI RAATTY 1 cut(s) 1613
XbaI TCTAGA 2 cut(s) 748, 1564
XceI RCATGY 2 cut(s) 174, 818
XspI CTAG 6 cut(s) 318, 327, 489, 650, 749, 1565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.