Rorug06G0334600

3beta-hydroxysteroid-dehydrogenase decarboxylase isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003789
Physical Location & Seq
Reverse (-)
48703108 .. 48707768
4661 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0334600.1

Sequence Viewer

Length: 1353 bp
ATGACGAAGAAGAGGGGGAGAGAGAGGCATCGAAATCCAAAGCCGTTCCTCGCCGAAGACGATGTCTCTGTGGCTTCCACAAAGCTAACCAAACAGCCCAAAATGGTGCGCAAGCGCCAAGAGAATCTAGTTTTATCAGCAGGCATGAGCTCGAAAATCCTCAGGGAAGCTCAGATTCAGGAGAAGGAGATCGAGGAAGAGGAGAACACAAACGCCTTCACTGAAGAGCCGCACAACGATGAAGAGGACGACGATGACTTTGATACGTTCCTTGAAACACATAGAGAGGATGAAGCAAACAAAGTGATGGAGGCCATAATGAATGAGACTGAAGATGAAGATGAGGAGAAGTTACTAGAGCGATTCTTATCAAAGGAGGAGGGTGATGATGGTCCACAGCGTAATCTCGCAGACCTCGTACTTGCAACCATTAAGAACAAACAGTATGATGTCACCTCAGATGCTAGGTCTTTCGTTAAAATGGATACAGATTTCATGGAAGTCTACAAGGGGGTTGCAAAGTTTATGAAAAGCTACAGTGCCGGCAAGATTGCCAAACCATTCAAATTTATCCCGGCAATGCAACAATGGGAGGATGCCCTCTATTTCACTGAGCCTGATAATTGGTCACCAACTGCTATGTATGAAGCTACAAGAATTCTTGCTTCTAATTTGACTCCGCAAAAAGCAGAACGTTTCTACAAGCATGTCTTGCTCCCACGGGTGAGACGAGACATTCGGAATAAGAAGAAGAAGAAGCCCTTGCATTTTTCCCTGTATCAATCTCTAAGGAAGTCCGTCCACAAGCGTGCTGCCTTTTTTAAAGGAATACTGTTGCCTTTGTGTGAGTCAAGGACATGCACTCTAAAAGAAGCCTCCGTTGTTGGGAGCATCATACAAAAGGTTCGAATTTCTGCAAGTCATGCAGGTGCTGCAATGATGATGCTAGCAGAGATGGAATATAGCAGCGCAAGAAGTTATTTTATAAAGCTTCTTTTGGAGAAACAGTATGCTTTGCCAAATCCTGTAATCGAGGCTCTTGTTGCACATTTTATGAGATCTGTGGAGGAACCAAGAGTAATGCCTGTGATATGGCACCAGTCTCTTCTTGCATTTGTGGAAAGGTACAAAAATGATATAACAAAGGACAACAAAGATAAGCTTAGGAATCTTCTTGAAGCACAAAACCACCGTGAGATTACTCCTGAAATAAGAAGGGAGCTATTTCATAGCTGCAATCATGGTGAGAAGGAGCATGATCGTGCGTCAAGTGATTCTCTGATGTCTGTGTTCAATAAACCAATCGATGAAGACATGTTTGATATTCCAGAGGTGCCTATGGAGGATGACTAA

Protein Analysis

450

Amino Acids

52.35

Weight (kDa)

5.91

Isoelectric Point (pI)

53.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bystin PF05291 132 - 417 2.2e-110 Bystin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 986
AarI CACCTGC 1 cut(s) 917
Acc16I TGCGCA 1 cut(s) 110
Acc36I ACCTGC 1 cut(s) 917
AccB1I GGYRCC 2 cut(s) 1095, 1333
AccI GTMKAC 1 cut(s) 504
AciI CCGC 2 cut(s) 230, 680
AclI AACGTT 1 cut(s) 694
AcsI RAATTY 3 cut(s) 566, 657, 909
AcuI CTGAAG 2 cut(s) 243, 351
AfaI GTAC 2 cut(s) 420, 1127
AfiI CCNNNNNNNGG 1 cut(s) 885
AflIII ACRYGT 1 cut(s) 1314
AgsI TTSAA 4 cut(s) 275, 565, 1178, 1294
AleI CACNNNNGTG 1 cut(s) 807
AluBI AGCT 9 cut(s) 85, 150, 170, 534, 650, 991, 1162, 1222, 1233
AluI AGCT 9 cut(s) 85, 150, 170, 534, 650, 991, 1162, 1222, 1233
Alw21I GWGCWC 1 cut(s) 152
Alw26I GTCTC 5 cut(s) 70, 320, 721, 726, 1107
AlwNI CAGNNNCTG 1 cut(s) 932
AoxI GGCC 1 cut(s) 312
ApeKI GCWGC 4 cut(s) 812, 932, 966, 1233
ApoI RAATTY 3 cut(s) 566, 657, 909
ArsI GACNNNNNNTTYG 6 cut(s) 48, 80, 242, 274, 720, 752
AspLEI GCGC 3 cut(s) 111, 117, 971
AspS9I GGNCC 1 cut(s) 392
AsuC2I CCSGG 1 cut(s) 575
AsuHPI GGTGA 5 cut(s) 395, 445, 621, 736, 1256
AsuII TTCGAA 1 cut(s) 907
AsuNHI GCTAGC 1 cut(s) 946
AvaII GGWCC 1 cut(s) 392
AxyI CCTNAGG 1 cut(s) 161
BanI GGYRCC 2 cut(s) 1095, 1333
BanII GRGCYC 1 cut(s) 152
BbsI GAAGAC 2 cut(s) 63, 1317
Bbv12I GWGCWC 1 cut(s) 152
BbvI GCAGC 4 cut(s) 799, 919, 978, 1220
BccI CCATC 3 cut(s) 301, 383, 949
BceAI ACGGC 1 cut(s) 28
BciVI GTATCC 1 cut(s) 478
BcnI CCSGG 1 cut(s) 575
BcoDI GTCTC 5 cut(s) 70, 320, 721, 726, 1107
BfaI CTAG 4 cut(s) 128, 356, 465, 947
BfmI CTRYAG 1 cut(s) 535
BfoI RGCGCY 1 cut(s) 118
BfuAI ACCTGC 1 cut(s) 917
BfuI GTATCC 1 cut(s) 478
BglII AGATCT 1 cut(s) 1058
BisI GCNGC 5 cut(s) 230, 813, 933, 967, 1234
BlsI GCNGC 5 cut(s) 231, 814, 934, 968, 1235
Bme1390I CCNGG 1 cut(s) 575
Bme18I GGWCC 1 cut(s) 392
BmgT120I GGNCC 1 cut(s) 392
BmiI GGNNCC 3 cut(s) 1071, 1097, 1335
BmrFI CCNGG 1 cut(s) 575
BmsI GCATC 5 cut(s) 37, 451, 586, 900, 933
BmtI GCTAGC 1 cut(s) 950
BpiI GAAGAC 2 cut(s) 63, 1317
Bpu10I CCTNAGC 1 cut(s) 1163
Bpu14I TTCGAA 1 cut(s) 907
BpuMI CCSGG 1 cut(s) 575
Bsa29I ATCGAT 1 cut(s) 1305
BsaBI GATNNNNATC 1 cut(s) 367
BsaJI CCNNGG 1 cut(s) 719
BsaXI ACNNNNNCTCC 2 cut(s) 992, 1022
Bsc4I CCNNNNNNNGG 1 cut(s) 885
Bse118I RCCGGY 1 cut(s) 542
Bse1I ACTGG 1 cut(s) 1099
Bse21I CCTNAGG 1 cut(s) 161
Bse3DI GCAATG 2 cut(s) 585, 942
Bse8I GATNNNNATC 1 cut(s) 367
BseCI ATCGAT 1 cut(s) 1305
BseDI CCNNGG 1 cut(s) 719
BseGI GGATG 3 cut(s) 295, 601, 1351
BseJI GATNNNNATC 1 cut(s) 367
BseLI CCNNNNNNNGG 1 cut(s) 885
BseMI GCAATG 2 cut(s) 585, 942
BseMII CTCAG 4 cut(s) 175, 185, 471, 603
BseNI ACTGG 1 cut(s) 1099
BseRI GAGGAG 3 cut(s) 215, 359, 392
BseXI GCAGC 4 cut(s) 799, 919, 978, 1220
BshFI GGCC 1 cut(s) 314
BshNI GGYRCC 2 cut(s) 1095, 1333
BshVI ATCGAT 1 cut(s) 1305
BsiHKAI GWGCWC 1 cut(s) 152
BsiSI CCGG 2 cut(s) 543, 575
BslI CCNNNNNNNGG 1 cut(s) 885
BsmAI GTCTC 5 cut(s) 70, 320, 721, 726, 1107
BsmBI CGTCTC 1 cut(s) 721
BsnI GGCC 1 cut(s) 314
Bsp119I TTCGAA 1 cut(s) 907
Bsp1286I GDGCHC 1 cut(s) 152
Bsp143I GATC 3 cut(s) 189, 1058, 1258
BspACI CCGC 2 cut(s) 230, 680
BspANI GGCC 1 cut(s) 314
BspCNI CTCAG 4 cut(s) 174, 184, 470, 604
BspDI ATCGAT 1 cut(s) 1305
BspLI GGNNCC 3 cut(s) 1071, 1097, 1335
BspMI ACCTGC 1 cut(s) 917
BspOI GCTAGC 1 cut(s) 950
BspQI GCTCTTC 1 cut(s) 219
BspT104I TTCGAA 1 cut(s) 907
BspT107I GGYRCC 2 cut(s) 1095, 1333
BsrDI GCAATG 2 cut(s) 585, 942
BsrFI RCCGGY 1 cut(s) 542
BsrI ACTGG 1 cut(s) 1099
BssAI RCCGGY 1 cut(s) 542
BssECI CCNNGG 1 cut(s) 719
BssMI GATC 3 cut(s) 189, 1058, 1258
Bst4CI ACNGT 5 cut(s) 444, 539, 834, 1008, 1193
Bst6I CTCTTC 5 cut(s) 5, 192, 219, 237, 1110
BstAPI GCANNNNNTGC 3 cut(s) 712, 923, 932
BstBI TTCGAA 1 cut(s) 907
BstC8I GCNNGC 5 cut(s) 113, 142, 544, 810, 948
BstDEI CTNAG 6 cut(s) 161, 171, 457, 612, 788, 1163
BstDSI CCRYGG 1 cut(s) 719
BstEII GGTNACC 1 cut(s) 627
BstF5I GGATG 3 cut(s) 295, 601, 1351
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 3 cut(s) 111, 117, 971
BstKTI GATC 3 cut(s) 192, 1061, 1261
BstMAI GTCTC 5 cut(s) 70, 320, 721, 726, 1107
BstMBI GATC 3 cut(s) 189, 1058, 1258
BstMWI GCNNNNNNNGC 4 cut(s) 712, 923, 932, 1043
BstNSI RCATGY 3 cut(s) 710, 861, 1318
BstPI GGTNACC 1 cut(s) 627
BstSCI CCNGG 1 cut(s) 573
BstSFI CTRYAG 1 cut(s) 535
BstV1I GCAGC 4 cut(s) 799, 919, 978, 1220
BstV2I GAAGAC 2 cut(s) 63, 1317
BstX2I RGATCY 1 cut(s) 1058
BstYI RGATCY 1 cut(s) 1058
Bsu15I ATCGAT 1 cut(s) 1305
Bsu36I CCTNAGG 1 cut(s) 161
BsuI GTATCC 1 cut(s) 478
BsuRI GGCC 1 cut(s) 314
BsuTUI ATCGAT 1 cut(s) 1305
BtgI CCRYGG 1 cut(s) 719
BtsCI GGATG 3 cut(s) 295, 601, 1351
BtsIMutI CAGTG 3 cut(s) 219, 544, 609
BveI ACCTGC 1 cut(s) 917
Cac8I GCNNGC 5 cut(s) 113, 142, 544, 810, 948
CaiI CAGNNNCTG 1 cut(s) 932
CfoI GCGC 3 cut(s) 111, 117, 971
Cfr10I RCCGGY 1 cut(s) 542
Cfr13I GGNCC 1 cut(s) 392
ClaI ATCGAT 1 cut(s) 1305
CseI GACGC 1 cut(s) 1254
Csp6I GTAC 2 cut(s) 419, 1126
CviAII CATG 8 cut(s) 145, 496, 707, 858, 923, 1241, 1256, 1315
CviQI GTAC 2 cut(s) 419, 1126
DdeI CTNAG 6 cut(s) 161, 171, 457, 612, 788, 1163
DpnI GATC 3 cut(s) 191, 1060, 1260
DpnII GATC 3 cut(s) 189, 1058, 1258
DraI TTTAAA 1 cut(s) 823
Eam1104I CTCTTC 5 cut(s) 5, 192, 219, 237, 1110
EarI CTCTTC 5 cut(s) 5, 192, 219, 237, 1110
Ecl136II GAGCTC 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 152
Eco47I GGWCC 1 cut(s) 392
Eco53kI GAGCTC 1 cut(s) 150
Eco57I CTGAAG 2 cut(s) 243, 351
Eco81I CCTNAGG 1 cut(s) 161
Eco91I GGTNACC 1 cut(s) 627
EcoICRI GAGCTC 1 cut(s) 150
EcoO65I GGTNACC 1 cut(s) 627
EcoRI GAATTC 1 cut(s) 657
EcoT38I GRGCYC 1 cut(s) 152
Esp3I CGTCTC 1 cut(s) 721
FaeI CATG 8 cut(s) 148, 499, 710, 861, 926, 1244, 1259, 1318
FalI AAGNNNNNCTT 6 cut(s) 695, 727, 746, 778, 1146, 1178
FatI CATG 8 cut(s) 144, 495, 706, 857, 922, 1240, 1255, 1314
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 5 cut(s) 230, 813, 933, 967, 1234
FokI GGATG 2 cut(s) 302, 608
FriOI GRGCYC 1 cut(s) 152
Fsp4HI GCNGC 5 cut(s) 230, 813, 933, 967, 1234
FspBI CTAG 4 cut(s) 128, 356, 465, 947
FspI TGCGCA 1 cut(s) 110
GlaI GCGC 3 cut(s) 110, 116, 970
GluI GCNGC 5 cut(s) 230, 813, 933, 967, 1234
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 1 cut(s) 314
HapII CCGG 2 cut(s) 543, 575
HgaI GACGC 1 cut(s) 1254
HhaI GCGC 3 cut(s) 111, 117, 971
Hin1II CATG 8 cut(s) 148, 499, 710, 861, 926, 1244, 1259, 1318
Hin6I GCGC 3 cut(s) 109, 115, 969
HinP1I GCGC 3 cut(s) 109, 115, 969
HindIII AAGCTT 2 cut(s) 989, 1160
HinfI GANTC 7 cut(s) 124, 175, 363, 676, 848, 1168, 1274
HpaII CCGG 2 cut(s) 543, 575
HphI GGTGA 5 cut(s) 395, 445, 621, 736, 1256
Hpy166II GTNNAC 3 cut(s) 395, 505, 802
Hpy188I TCNGA 4 cut(s) 174, 460, 741, 1281
Hpy188III TCNNGA 4 cut(s) 179, 1175, 1205, 1328
Hpy8I GTNNAC 3 cut(s) 395, 505, 802
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 4 cut(s) 178, 226, 1209, 1243
HpyCH4III ACNGT 5 cut(s) 444, 539, 834, 1008, 1193
HpyCH4IV ACGT 2 cut(s) 266, 694
HpyF10VI GCNNNNNNNGC 4 cut(s) 712, 923, 932, 1043
HpyF3I CTNAG 6 cut(s) 161, 171, 457, 612, 788, 1163
HpySE526I ACGT 2 cut(s) 266, 694
Hsp92II CATG 8 cut(s) 148, 499, 710, 861, 926, 1244, 1259, 1318
HspAI GCGC 3 cut(s) 109, 115, 969
KroI GCCGGC 1 cut(s) 542
KroNI GCCGGC 1 cut(s) 544
Kzo9I GATC 3 cut(s) 189, 1058, 1258
LguI GCTCTTC 1 cut(s) 219
LmnI GCTCC 4 cut(s) 720, 888, 1219, 1252
Lsp1109I GCAGC 4 cut(s) 799, 919, 978, 1220
LweI GCATC 5 cut(s) 37, 451, 586, 900, 933
MaeI CTAG 4 cut(s) 128, 356, 465, 947
MaeII ACGT 2 cut(s) 266, 694
MaeIII GTNAC 3 cut(s) 351, 451, 627
MalI GATC 3 cut(s) 191, 1060, 1260
MboI GATC 3 cut(s) 189, 1058, 1258
MflI RGATCY 1 cut(s) 1058
MhlI GDGCHC 1 cut(s) 152
MluCI AATT 5 cut(s) 566, 622, 657, 670, 909
MlyI GAGTC 2 cut(s) 670, 857
MroNI GCCGGC 1 cut(s) 542
MseI TTAA 3 cut(s) 432, 477, 822
MslI CAYNNNNRTG 4 cut(s) 237, 807, 927, 1260
MspI CCGG 2 cut(s) 543, 575
MspR9I CCNGG 1 cut(s) 575
MwoI GCNNNNNNNGC 4 cut(s) 712, 923, 932, 1043
NaeI GCCGGC 1 cut(s) 544
NciI CCSGG 1 cut(s) 575
NdeII GATC 3 cut(s) 189, 1058, 1258
NgoMIV GCCGGC 1 cut(s) 542
NheI GCTAGC 1 cut(s) 946
NlaIII CATG 8 cut(s) 148, 499, 710, 861, 926, 1244, 1259, 1318
NlaIV GGNNCC 3 cut(s) 1071, 1097, 1335
NmuCI GTSAC 2 cut(s) 451, 627
NsbI TGCGCA 1 cut(s) 110
NspI RCATGY 3 cut(s) 710, 861, 1318
NspV TTCGAA 1 cut(s) 907
OliI CACNNNNGTG 1 cut(s) 807
PaqCI CACCTGC 1 cut(s) 917
PciI ACATGT 1 cut(s) 1314
PciSI GCTCTTC 1 cut(s) 219
PcsI WCGNNNNNNNCGW 3 cut(s) 57, 414, 727
PdiI GCCGGC 1 cut(s) 544
PfeI GAWTC 5 cut(s) 124, 175, 363, 1168, 1274
PflFI GACNNNGTC 1 cut(s) 62
PkrI GCNGC 5 cut(s) 231, 814, 934, 968, 1235
PleI GAGTC 2 cut(s) 670, 856
PpsI GAGTC 2 cut(s) 670, 856
PscI ACATGT 1 cut(s) 1314
PsiI TTATAA 1 cut(s) 986
Psp124BI GAGCTC 1 cut(s) 152
Psp1406I AACGTT 1 cut(s) 694
PspEI GGTNACC 1 cut(s) 627
PspN4I GGNNCC 3 cut(s) 1071, 1097, 1335
PspPI GGNCC 1 cut(s) 392
PstNI CAGNNNCTG 1 cut(s) 932
PsuI RGATCY 1 cut(s) 1058
PsyI GACNNNGTC 1 cut(s) 62
RsaI GTAC 2 cut(s) 420, 1127
RsaNI GTAC 2 cut(s) 419, 1126
RseI CAYNNNNRTG 4 cut(s) 237, 807, 927, 1260
SacI GAGCTC 1 cut(s) 152
SapI GCTCTTC 1 cut(s) 219
SaqAI TTAA 3 cut(s) 432, 477, 822
SatI GCNGC 5 cut(s) 230, 813, 933, 967, 1234
Sau3AI GATC 3 cut(s) 189, 1058, 1258
Sau96I GGNCC 1 cut(s) 392
SchI GAGTC 2 cut(s) 670, 857
ScrFI CCNGG 1 cut(s) 575
SduI GDGCHC 1 cut(s) 152
SfaNI GCATC 5 cut(s) 37, 451, 586, 900, 933
SfcI CTRYAG 1 cut(s) 535
SfuI TTCGAA 1 cut(s) 907
SinI GGWCC 1 cut(s) 392
SmiMI CAYNNNNRTG 4 cut(s) 237, 807, 927, 1260
Sse9I AATT 5 cut(s) 566, 622, 657, 670, 909
SsiI CCGC 2 cut(s) 230, 680
SspMI CTAG 4 cut(s) 128, 356, 465, 947
SstI GAGCTC 1 cut(s) 152
StyD4I CCNGG 1 cut(s) 573
TaaI ACNGT 5 cut(s) 444, 539, 834, 1008, 1193
TaiI ACGT 2 cut(s) 269, 697
TaqI TCGA 6 cut(s) 31, 152, 192, 907, 1032, 1305
TasI AATT 5 cut(s) 566, 622, 657, 670, 909
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 5 cut(s) 124, 175, 363, 1168, 1274
Tru1I TTAA 3 cut(s) 432, 477, 822
Tru9I TTAA 3 cut(s) 432, 477, 822
TscAI CASTG 3 cut(s) 226, 544, 616
TseFI GTSAC 2 cut(s) 451, 627
TseI GCWGC 4 cut(s) 812, 932, 966, 1233
Tsp45I GTSAC 2 cut(s) 451, 627
TspDTI ATGAA 9 cut(s) 255, 306, 335, 351, 484, 542, 660, 1217, 1323
TspGWI ACGGA 2 cut(s) 787, 868
TspRI CASTG 3 cut(s) 226, 544, 616
Tth111I GACNNNGTC 1 cut(s) 62
VpaK11BI GGWCC 1 cut(s) 392
XapI RAATTY 3 cut(s) 566, 657, 909
XceI RCATGY 3 cut(s) 710, 861, 1318
XmiI GTMKAC 1 cut(s) 504
XspI CTAG 4 cut(s) 128, 356, 465, 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.