Rorug06G0334800

3beta-hydroxysteroid-dehydrogenase decarboxylase isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003789
Physical Location & Seq
Reverse (-)
48727820 .. 48729859
2040 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0334800.1

Sequence Viewer

Length: 1494 bp
ATGAATTTCTTCAAGTCCGTATTCGCAGACGAGCCCGATCCGTCAAACCCCGAATCCGAATCGGGGCCCACCAGTAACCAAGATCCAGATCCGAAGCCCCAACCGGATCCCAACCCAGCCGGCATTGGCGGGTGGAACTTGGGCGATCTGATCAAAACCATCGCCACCAAGTCCGAGACCGTCATCGAGACGTACCGCCGCGACTTGCAGGAATTCGGGTCGGGTCTGAAGAAAGAGATCGAGGTCGCCCAGGGGTCGCTGGAGACAGTCGGCACCAGAATCGATGAGTTGGGCAACACAGTCCTCGCAGGAACGGCCCAGATCATCTCCCAGGGCATCCTCGCCCCCGATCACGAATCCGATTCGTCCGATTCCAACAACACCTCGACCCCCCAGCGGAGGAGCTTGAATTCCAACAAGTACAGTAGGTTCGAGTCTCAGGTCCGCGCGATTCAGGGTGACGTCAGCACCTACTGTGAGGAGCCTGAGGATTTGGAGGAGTATAGGAAGTGGAGGAAATCGGGGTTTGTGTTGGAGGAGAAGAGGGAGGAGATTGAGGGTTTGCTTGAGGGGAATGGAGCAATGGAGAGTATTTACAACAAGGTCGCGCCGAATGTGGTTGATGATGAGACGTTTTGGAGCAGGTATTATTTTAGGGTGTATAAGCTCAAGCAAGCTGAGGATGTTAGGGCGAATCTGGTGAAGAGGGCGATTTCGGTTGAATTGGAGGAGGAGTTGAGTTGGGATGTTGATGATGATGAGTATGATGAAAGTGATGGCAATGTGGTTGCGAAATCGAGTTTAGGGAAGAGTAGTGAAGTTGGTGGTGGAGTTGTGAAGGAGAAGGAGTTGAAGAGTGATGATCAGGTTGGAGATAAGGGTTCCTCAAAGGTGGGAGAGGTGAGCAGTGATGTGGCAGCGAAAACAGAGTCGGTTAGCAAAGATTCGGAGCAAAAGATTGTCAAGGAAGAGAGTTCCGGTGAGAAATTGCAAAGTGGGACTTCGGAGGTTGTTGAAGAGAAGGAAGGGAAGAGTCTGAAAGTCGATAATGAGAAGAAAGAAAGTGCTGTGGAGTCTAGTGGTGATAAGGAGTCGGAGAAGAAAGTGAATGAAGTAGGGGATTCTGGGATGCCATCAAAATCTGATGAGGAGAAGAAAGTGAATGTACAAGGGGATTCTGGGAAGAAAGACTTGCCGCCATCAAAATCGGATGATAAAGCGGCTTTAGAGGTGAAGAATGAGGCTCCTGCATCTAGCAAAGAGAGTGGTGTATCGGCTCCTGCTTCAGTCCGGCCCTCAGTAGAACCTGAGGAAGAAGACCTTGGCTGGGATGAGATTGAGGATCTTAGCAGCATTGATGAAAAGAAAGCGAACCCTGGTGCAAGTGGTGGAAGTGCTAATAGAGCTGAGCTGCAGAAGCGGCTCAGCACTGCGGAAGAAGAAGAAGACTTGAGCTGGGATATAGATGATGATGATGAACCCGCCAAAGCTTGA

Protein Analysis

497

Amino Acids

54.18

Weight (kDa)

4.42

Isoelectric Point (pI)

52.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 173 - 228 3.5e-14 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 465
Acc36I ACCTGC 1 cut(s) 633
AccB1I GGYRCC 1 cut(s) 272
AccII CGCG 4 cut(s) 201, 447, 449, 608
AclWI GGATC 6 cut(s) 32, 77, 83, 101, 114, 1350
AcsI RAATTY 3 cut(s) 4, 212, 409
AcuI CTGAAG 2 cut(s) 248, 1269
AcyI GRCGYC 1 cut(s) 462
AfaI GTAC 3 cut(s) 194, 422, 1167
AfiI CCNNNNNNNGG 4 cut(s) 63, 396, 399, 1327
AgsI TTSAA 5 cut(s) 13, 409, 722, 853, 1016
AjnI CCWGG 3 cut(s) 249, 330, 1375
AjuI GAANNNNNNNTTGG 2 cut(s) 1305, 1337
AluBI AGCT 7 cut(s) 405, 667, 677, 1406, 1411, 1455, 1490
AluI AGCT 7 cut(s) 405, 667, 677, 1406, 1411, 1455, 1490
Alw26I GTCTC 5 cut(s) 170, 182, 257, 441, 623
AlwI GGATC 6 cut(s) 32, 77, 83, 101, 114, 1350
AoxI GGCC 3 cut(s) 65, 315, 1292
ApaI GGGCCC 1 cut(s) 69
ApeKI GCWGC 3 cut(s) 917, 1350, 1411
ApoI RAATTY 3 cut(s) 4, 212, 409
ArsI GACNNNNNNTTYG 2 cut(s) 914, 946
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 2 cut(s) 449, 610
AspS9I GGNCC 5 cut(s) 65, 66, 316, 442, 1293
AsuHPI GGTGA 6 cut(s) 470, 712, 913, 992, 1094, 1243
AvaII GGWCC 1 cut(s) 442
AxyI CCTNAGG 2 cut(s) 486, 1308
BaeGI GKGCMC 1 cut(s) 69
BamHI GGATCC 1 cut(s) 106
BanI GGYRCC 1 cut(s) 272
BanII GRGCYC 2 cut(s) 36, 69
BbsI GAAGAC 2 cut(s) 1323, 1452
BbvCI CCTCAGC 1 cut(s) 678
BbvI GCAGC 3 cut(s) 929, 1362, 1398
BccI CCATC 4 cut(s) 167, 770, 1141, 1207
BceAI ACGGC 1 cut(s) 330
BcgI CGANNNNNNTGC 2 cut(s) 262, 296
BciT130I CCWGG 3 cut(s) 251, 332, 1377
BclI TGATCA 2 cut(s) 150, 862
BcoDI GTCTC 5 cut(s) 170, 182, 257, 441, 623
BfaI CTAG 2 cut(s) 1077, 1254
BfmI CTRYAG 1 cut(s) 1412
BfuAI ACCTGC 1 cut(s) 633
BisI GCNGC 7 cut(s) 199, 918, 1196, 1221, 1351, 1412, 1421
BlpI GCTNAGC 2 cut(s) 1407, 1424
BlsI GCNGC 7 cut(s) 200, 919, 1197, 1222, 1352, 1413, 1422
Bme1390I CCNGG 3 cut(s) 251, 332, 1377
Bme18I GGWCC 1 cut(s) 442
BmgT120I GGNCC 5 cut(s) 65, 66, 316, 442, 1293
BmiI GGNNCC 8 cut(s) 66, 67, 108, 274, 483, 883, 1245, 1278
BmrFI CCNGG 3 cut(s) 251, 332, 1377
BmsI GCATC 3 cut(s) 345, 1119, 1259
BpiI GAAGAC 2 cut(s) 1323, 1452
BpmI CTGGAG 1 cut(s) 281
Bpu10I CCTNAGC 1 cut(s) 678
Bpu1102I GCTNAGC 2 cut(s) 1407, 1424
BpuEI CTTGAG 3 cut(s) 587, 653, 1471
Bsa29I ATCGAT 1 cut(s) 282
BsaBI GATNNNNATC 1 cut(s) 87
BsaHI GRCGYC 1 cut(s) 462
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 6 cut(s) 249, 250, 330, 331, 1321, 1375
BsaWI WCCGGW 2 cut(s) 103, 977
BsaXI ACNNNNNCTCC 4 cut(s) 719, 749, 833, 863
Bsc4I CCNNNNNNNGG 4 cut(s) 63, 396, 399, 1327
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 1 cut(s) 72
Bse21I CCTNAGG 2 cut(s) 486, 1308
Bse3DI GCAATG 2 cut(s) 588, 787
Bse8I GATNNNNATC 1 cut(s) 87
BseBI CCWGG 3 cut(s) 251, 332, 1377
BseCI ATCGAT 1 cut(s) 282
BseDI CCNNGG 6 cut(s) 249, 250, 330, 331, 1321, 1375
BseGI GGATG 6 cut(s) 336, 688, 751, 1134, 1216, 1336
BseJI GATNNNNATC 1 cut(s) 87
BseLI CCNNNNNNNGG 4 cut(s) 63, 396, 399, 1327
BseMI GCAATG 2 cut(s) 588, 787
BseMII CTCAG 7 cut(s) 452, 477, 669, 1299, 1311, 1398, 1438
BseNI ACTGG 1 cut(s) 72
BseRI GAGGAG 8 cut(s) 415, 494, 512, 551, 563, 743, 746, 1163
BseSI GKGCMC 1 cut(s) 69
BseXI GCAGC 3 cut(s) 929, 1362, 1398
BseYI CCCAGC 4 cut(s) 115, 393, 1326, 1455
Bsh1236I CGCG 4 cut(s) 201, 447, 449, 608
BshFI GGCC 3 cut(s) 67, 317, 1294
BshNI GGYRCC 1 cut(s) 272
BshVI ATCGAT 1 cut(s) 282
BsiSI CCGG 4 cut(s) 104, 120, 978, 1291
BslFI GGGAC 1 cut(s) 1012
BslI CCNNNNNNNGG 4 cut(s) 63, 396, 399, 1327
BsmAI GTCTC 5 cut(s) 170, 182, 257, 441, 623
BsmBI CGTCTC 2 cut(s) 182, 623
BsmFI GGGAC 1 cut(s) 1012
BsnI GGCC 3 cut(s) 67, 317, 1294
Bso31I GGTCTC 1 cut(s) 170
Bsp120I GGGCCC 1 cut(s) 65
Bsp1286I GDGCHC 2 cut(s) 36, 69
Bsp1407I TGTACA 1 cut(s) 1165
Bsp1720I GCTNAGC 2 cut(s) 1407, 1424
BspANI GGCC 3 cut(s) 67, 317, 1294
BspCNI CTCAG 7 cut(s) 451, 478, 670, 1300, 1310, 1399, 1437
BspDI ATCGAT 1 cut(s) 282
BspFNI CGCG 4 cut(s) 201, 447, 449, 608
BspLI GGNNCC 8 cut(s) 66, 67, 108, 274, 483, 883, 1245, 1278
BspMAI CTGCAG 1 cut(s) 1416
BspMI ACCTGC 1 cut(s) 633
BspPI GGATC 6 cut(s) 32, 77, 83, 101, 114, 1350
BspT107I GGYRCC 1 cut(s) 272
BspTNI GGTCTC 1 cut(s) 170
BsrDI GCAATG 2 cut(s) 588, 787
BsrFI RCCGGY 1 cut(s) 119
BsrGI TGTACA 1 cut(s) 1165
BsrI ACTGG 1 cut(s) 72
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 6 cut(s) 249, 250, 330, 331, 1321, 1375
BssNI GRCGYC 1 cut(s) 462
BssT1I CCWWGG 1 cut(s) 1321
Bst2UI CCWGG 3 cut(s) 251, 332, 1377
Bst4CI ACNGT 5 cut(s) 181, 268, 301, 425, 476
Bst6I CTCTTC 7 cut(s) 536, 698, 803, 848, 963, 1011, 1025
BstACI GRCGYC 1 cut(s) 462
BstAUI TGTACA 1 cut(s) 1165
BstC8I GCNNGC 2 cut(s) 121, 675
BstDEI CTNAG 8 cut(s) 438, 486, 678, 1297, 1308, 1346, 1407, 1424
BstF5I GGATG 6 cut(s) 336, 688, 751, 1134, 1216, 1336
BstFNI CGCG 4 cut(s) 201, 447, 449, 608
BstHHI GCGC 2 cut(s) 449, 610
BstMAI GTCTC 5 cut(s) 170, 182, 257, 441, 623
BstMWI GCNNNNNNNGC 4 cut(s) 314, 1403, 1417, 1420
BstNI CCWGG 3 cut(s) 251, 332, 1377
BstSCI CCNGG 3 cut(s) 249, 330, 1375
BstSFI CTRYAG 1 cut(s) 1412
BstSLI GKGCMC 1 cut(s) 69
BstUI CGCG 4 cut(s) 201, 447, 449, 608
BstV1I GCAGC 3 cut(s) 929, 1362, 1398
BstV2I GAAGAC 2 cut(s) 1323, 1452
BstX2I RGATCY 4 cut(s) 82, 88, 106, 1342
BstYI RGATCY 4 cut(s) 82, 88, 106, 1342
Bsu15I ATCGAT 1 cut(s) 282
Bsu36I CCTNAGG 2 cut(s) 486, 1308
BsuRI GGCC 3 cut(s) 67, 317, 1294
BsuTUI ATCGAT 1 cut(s) 282
BtgZI GCGATG 1 cut(s) 145
BtsCI GGATG 6 cut(s) 336, 688, 751, 1134, 1216, 1336
BtsI GCAGTG 2 cut(s) 913, 1428
BtsIMutI CAGTG 2 cut(s) 913, 1428
BveI ACCTGC 1 cut(s) 633
Cac8I GCNNGC 2 cut(s) 121, 675
CfoI GCGC 2 cut(s) 449, 610
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 5 cut(s) 65, 66, 316, 442, 1293
ClaI ATCGAT 1 cut(s) 282
Csp6I GTAC 3 cut(s) 193, 421, 1166
CspCI CAANNNNNGTGG 2 cut(s) 1246, 1281
CviQI GTAC 3 cut(s) 193, 421, 1166
DdeI CTNAG 8 cut(s) 438, 486, 678, 1297, 1308, 1346, 1407, 1424
Eam1104I CTCTTC 7 cut(s) 536, 698, 803, 848, 963, 1011, 1025
EarI CTCTTC 7 cut(s) 536, 698, 803, 848, 963, 1011, 1025
Eco130I CCWWGG 1 cut(s) 1321
Eco24I GRGCYC 2 cut(s) 36, 69
Eco31I GGTCTC 1 cut(s) 170
Eco47I GGWCC 1 cut(s) 442
Eco57I CTGAAG 2 cut(s) 248, 1269
Eco81I CCTNAGG 2 cut(s) 486, 1308
EcoO109I RGGNCCY 1 cut(s) 65
EcoRI GAATTC 2 cut(s) 212, 409
EcoRII CCWGG 3 cut(s) 249, 330, 1375
EcoT14I CCWWGG 1 cut(s) 1321
EcoT38I GRGCYC 2 cut(s) 36, 69
ErhI CCWWGG 1 cut(s) 1321
Esp3I CGTCTC 2 cut(s) 182, 623
FaiI YATR 4 cut(s) 504, 663, 765, 1463
FalI AAGNNNNNCTT 6 cut(s) 985, 1017, 1175, 1207, 1305, 1337
FaqI GGGAC 1 cut(s) 1012
FauI CCCGC 2 cut(s) 122, 1489
FbaI TGATCA 2 cut(s) 150, 862
Fnu4HI GCNGC 7 cut(s) 199, 918, 1196, 1221, 1351, 1412, 1421
FokI GGATG 6 cut(s) 323, 695, 758, 1141, 1223, 1343
FriOI GRGCYC 2 cut(s) 36, 69
Fsp4HI GCNGC 7 cut(s) 199, 918, 1196, 1221, 1351, 1412, 1421
FspBI CTAG 2 cut(s) 1077, 1254
GlaI GCGC 2 cut(s) 448, 609
GluI GCNGC 7 cut(s) 199, 918, 1196, 1221, 1351, 1412, 1421
GsaI CCCAGC 4 cut(s) 119, 397, 1330, 1459
GsuI CTGGAG 1 cut(s) 281
HaeIII GGCC 3 cut(s) 67, 317, 1294
HapII CCGG 4 cut(s) 104, 120, 978, 1291
HhaI GCGC 2 cut(s) 449, 610
Hin1I GRCGYC 1 cut(s) 462
Hin6I GCGC 2 cut(s) 447, 608
HinP1I GCGC 2 cut(s) 447, 608
HindIII AAGCTT 1 cut(s) 1488
HpaII CCGG 4 cut(s) 104, 120, 978, 1291
HphI GGTGA 6 cut(s) 470, 712, 913, 992, 1094, 1243
Hpy188III TCNNGA 3 cut(s) 86, 187, 353
HpyAV CCTTC 4 cut(s) 832, 838, 1015, 1019
HpyCH4III ACNGT 5 cut(s) 181, 268, 301, 425, 476
HpyCH4IV ACGT 3 cut(s) 191, 462, 632
HpyCH4V TGCA 5 cut(s) 208, 991, 1250, 1382, 1414
HpyF10VI GCNNNNNNNGC 4 cut(s) 314, 1403, 1417, 1420
HpyF3I CTNAG 8 cut(s) 438, 486, 678, 1297, 1308, 1346, 1407, 1424
HpySE526I ACGT 3 cut(s) 191, 462, 632
Hsp92I GRCGYC 1 cut(s) 462
HspAI GCGC 2 cut(s) 447, 608
KroI GCCGGC 1 cut(s) 119
KroNI GCCGGC 1 cut(s) 121
Ksp22I TGATCA 2 cut(s) 150, 862
LmnI GCTCC 7 cut(s) 402, 481, 578, 639, 949, 1249, 1282
Lsp1109I GCAGC 3 cut(s) 929, 1362, 1398
LweI GCATC 3 cut(s) 345, 1119, 1259
MaeI CTAG 2 cut(s) 1077, 1254
MaeII ACGT 3 cut(s) 191, 462, 632
MaeIII GTNAC 2 cut(s) 74, 458
MflI RGATCY 4 cut(s) 82, 88, 106, 1342
MhlI GDGCHC 2 cut(s) 36, 69
MluCI AATT 5 cut(s) 4, 212, 409, 722, 986
MlyI GAGTC 5 cut(s) 443, 938, 1042, 1082, 1100
MmeI TCCRAC 5 cut(s) 399, 438, 513, 850, 1074
MroNI GCCGGC 1 cut(s) 119
MroXI GAANNNNTTC 1 cut(s) 8
MspA1I CMGCKG 1 cut(s) 397
MspI CCGG 4 cut(s) 104, 120, 978, 1291
MspR9I CCNGG 3 cut(s) 251, 332, 1377
MvaI CCWGG 3 cut(s) 251, 332, 1377
MvnI CGCG 4 cut(s) 201, 447, 449, 608
MwoI GCNNNNNNNGC 4 cut(s) 314, 1403, 1417, 1420
NaeI GCCGGC 1 cut(s) 121
NgoMIV GCCGGC 1 cut(s) 119
NlaIV GGNNCC 8 cut(s) 66, 67, 108, 274, 483, 883, 1245, 1278
NmuCI GTSAC 1 cut(s) 458
PasI CCCWGGG 2 cut(s) 250, 331
PdiI GCCGGC 1 cut(s) 121
PdmI GAANNNNTTC 1 cut(s) 8
PkrI GCNGC 7 cut(s) 200, 919, 1197, 1222, 1352, 1413, 1422
PleI GAGTC 5 cut(s) 442, 937, 1041, 1081, 1099
PpsI GAGTC 5 cut(s) 442, 937, 1041, 1081, 1099
Psp6I CCWGG 3 cut(s) 249, 330, 1375
PspFI CCCAGC 4 cut(s) 115, 393, 1326, 1455
PspGI CCWGG 3 cut(s) 249, 330, 1375
PspN4I GGNNCC 8 cut(s) 66, 67, 108, 274, 483, 883, 1245, 1278
PspOMI GGGCCC 1 cut(s) 65
PspPI GGNCC 5 cut(s) 65, 66, 316, 442, 1293
PsrI GAACNNNNNNTAC 2 cut(s) 413, 445
PstI CTGCAG 1 cut(s) 1416
PsuI RGATCY 4 cut(s) 82, 88, 106, 1342
RsaI GTAC 3 cut(s) 194, 422, 1167
RsaNI GTAC 3 cut(s) 193, 421, 1166
SatI GCNGC 7 cut(s) 199, 918, 1196, 1221, 1351, 1412, 1421
Sau96I GGNCC 5 cut(s) 65, 66, 316, 442, 1293
SchI GAGTC 5 cut(s) 443, 938, 1042, 1082, 1100
ScrFI CCNGG 3 cut(s) 251, 332, 1377
SduI GDGCHC 2 cut(s) 36, 69
SfaNI GCATC 3 cut(s) 345, 1119, 1259
SfcI CTRYAG 1 cut(s) 1412
SinI GGWCC 1 cut(s) 442
SmlI CTYRAG 3 cut(s) 566, 668, 1450
SmoI CTYRAG 3 cut(s) 566, 668, 1450
Sse9I AATT 5 cut(s) 4, 212, 409, 722, 986
SspMI CTAG 2 cut(s) 1077, 1254
StyD4I CCNGG 3 cut(s) 249, 330, 1375
StyI CCWWGG 1 cut(s) 1321
TaaI ACNGT 5 cut(s) 181, 268, 301, 425, 476
TaiI ACGT 3 cut(s) 194, 465, 635
TaqI TCGA 7 cut(s) 186, 240, 282, 386, 432, 797, 1044
TasI AATT 5 cut(s) 4, 212, 409, 722, 986
TatI WGTACW 2 cut(s) 420, 1165
TauI GCSGC 4 cut(s) 201, 1198, 1223, 1423
TscAI CASTG 2 cut(s) 913, 1435
TseFI GTSAC 1 cut(s) 458
TseI GCWGC 3 cut(s) 917, 1350, 1411
Tsp45I GTSAC 1 cut(s) 458
TspDTI ATGAA 5 cut(s) 17, 783, 1125, 1374, 1491
TspGWI ACGGA 2 cut(s) 7, 30
TspRI CASTG 2 cut(s) 913, 1435
VpaK11BI GGWCC 1 cut(s) 442
XapI RAATTY 3 cut(s) 4, 212, 409
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 2 cut(s) 1077, 1254
ZraI GACGTC 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.