Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0003789

Physical Location & Seq

Genomic Coordinates Forward (+)

27289605 .. 27295202

Gene Structure 5598 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G011530.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1728 bp

ATGACCTGCGATGAGAGATGGTGCGTCACCGTCTTCCATGTCCGAATCGCTGACCTCGGCCCCACTATCGATCTCGAACCTTCCGAGTCCAACGGAGTCCTCGCTGATGCTTTGAACTTCGGCCGTGCTGAGTACATTTCCATCGACCTCCGCTGCAAGGATCAAGTTCTTCAAGCATGTCAAGGTGCTGAGGTTGTGTTCCATATGGCGGCACCTAATTCGTCGATCAACAATTACCAGCTCCACCATTCGGTCCATGTGGAAGGAACTAAAAATGTGATTGATGCTTGTGTTAAGCAGAAAGTGAAGAGGCTCATATACACTAGCTCTCCTAGCGTTGTGTTTGATGGGGTTCATGGTATTATTGATGGGGATGAGTTGTTGTCATATCCACCTAAGCACAATGATACTTATTCGGCCACTAAAGCGCAAGGAGAAGAGTTGGTAATCAAGGCAAACGGTGTTAAAGGGCTTCTAACCTGCTGCATACGCCCTAGCAGCATTTTCGGACCCGGTGATAGGTTACTTGTTCCGTCTTTAGTTGTTGCCGCCAGGGCAGGGAAATCCAAGTTCATTATAGGAGATGGCAATAACATTTATGATTTCACGTATGTTGAAAACGTGGCCCATGCCCATATATGTGCAGAGCGAGCTCTAGCATTTGAAGGCACAGTTGCAGAGAAAGCTGCAGGACAGGCATATTTTATAACCAATATGGAACCTATTAAGTTTTGGGAGTTTATTTCACTTATTCTAGAAGGTCTTGGTTATGAAAGACCAAGGATAAAAATCCCTACAGCTGTTATGATGCCAATTGCACATGTGGTGGAGTGCACATATAAGCTTTTGGGCCCGTATGGGATGAAGGTTCCACAGTTGACGCCTTCAAGAATTAGACTTCTCTCTTGCACCAGATCTTTTAAATCTTCAAAAGCACAGGATCGAATTGGTTACACACCCATTATTTCGCTTCAGGAGGGTCTTAAGAGGACAATTGAGTCATACCCACACTTGAGGGCTGAACATCGACCCAAAAGAGAAGGGCCATCTAAAGCTTCTGTATATCTTGGAAGCGGAAGGGTTGCTGACGCATTACTGTGGAAGGATAAAAAACAGACATTCAAGGCACTATTAGTTTTGATTGCGATCTACTACAACTTCATTGAATCTAAAACTACGATTATTACTGTCATTTCGAAGAGTCTCATGTTCGCATCAATTTTCATGTTCATCTCTGGCAATTTACCAGAAATGATATTAGGATATAAACTCGAGAAGCTTCAATCATCACATTTTCACTTCTCAGAAGAGATGTCACAACAAATTGCTGCATTTGTGGTCTCATCATGGAATATATCTGTCAAAACTTTAAAATCTCTCGGGAAAGGAAATGACTGCATATTGTTTCTTAAGGCGGCTGTATCTCTACTGGTTCTTAGCTTTGTTGGAGCTATTTCACTTCAGAACTCATTTATTATAGGAGTTTCCACTGCCTTTTTAGCCTTCTTTGTGTATGAAAAGCAGGAAGAAAAAATCGATGGCATGGTTCTAGAAGCTTTCTCTCGTGGACTCAAATTGAAAACACAAAACCAAAAAGTTGATTCATGTTCTCAAATTCGTAGCAACATTCATATAAAAACCCTAAAAGTTTCGAACCATTCAAGATTAATATACAAAAGATTTCTATCATGCAAATTTAATCAAACACTCACACAAAAGCAACCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0008150 GO:0008152 GO:0016020 GO:0016491 GO:0016614 GO:0016616 GO:0047012 GO:0055114

KEGG Pathways

Metabolic & Signaling

ko00100 ko01100 ko01130 map00100 map01100 map01130

Pfam Domains

Protein Families

3Beta_HSD Reticulon

Protein Analysis

575

Amino Acids

64.0

Weight (kDa)

9.14

Isoelectric Point (pI)

39.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
3Beta_HSD	PF01073	27 - 264	3.2e-68	3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase	PF01370	38 - 216	7.5e-28	NAD dependent epimerase/dehydratase family
NAD_binding_10	PF13460	41 - 114	3e-07	NAD(P)H-binding
GDP_Man_Dehyd	PF16363	46 - 213	5.3e-08	GDP-mannose 4,6 dehydratase
NAD_binding_4	PF07993	58 - 209	3.7e-12	Male sterility protein
Reticulon	PF02453	361 - 516	4.6e-30	Reticulon

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003789)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47290 AT1G47290 AT2G26260 AT2G26260
fragaria_vesca	FvH4_2g36970 FvH4_2g36970
malus_domestica	MD08G1046500.v1.1
prunus_persica	Prupe.1G401700_v2.0.a1 Prupe.1G401700_v2.0.a1
pyrus_communis	pycom08g03860 pycom15g04580 pycom15g04640
rosa_chinensis	RchiOBHm_Chr4g0406951 RchiOBHm_Chr6g0304791
rosa_laevigata	RLG00000008747 RLG00000010949
rosa_multiflora	Rmu_sc0000600.1_g000025 Rmu_sc0003120.1_g000003
rosa_roxburghii	Rroxscaffold_7G00163470
rosa_rugosa	Rorug03G0259800 Rorug04G0075400 Rorug06G0334600 Rorug06G0334600 Rorug06G0334700 Rorug06G0334800
rosa_samantha	Rh4AG141600 Rh4BG138400 Rh4DG135500 Rh6AG446500 Rh6BG455100 Rh6CG460000 Rh6DG446800
rosa_wichuraiana	Rw4G011530 Rw6G038850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	707
AasI	GACNNNNNNGTC	1 cut(s)	997
Acc36I	ACCTGC	2 cut(s)	14, 488
AccB1I	GGYRCC	1 cut(s)	211
AciI	CCGC	5 cut(s)	151, 209, 549, 1074, 1415
AclWI	GGATC	2 cut(s)	168, 948
AcoI	YGGCCR	2 cut(s)	121, 417
AcsI	RAATTY	2 cut(s)	1614, 1694
AcuI	CTGAAG	2 cut(s)	956, 1445
AcyI	GRCGYC	1 cut(s)	881
AfaI	GTAC	1 cut(s)	134
AfiI	CCNNNNNNNGG	5 cut(s)	157, 208, 250, 519, 558
AflII	CTTAAG	2 cut(s)	983, 1409
AflIII	ACRYGT	1 cut(s)	820
AjnI	CCWGG	1 cut(s)	551
Alw21I	GWGCWC	2 cut(s)	655, 836
Alw26I	GTCTC	2 cut(s)	1208, 1345
Alw44I	GTGCAC	1 cut(s)	832
AlwI	GGATC	2 cut(s)	168, 948
Ama87I	CYCGRG	2 cut(s)	1271, 1379
AoxI	GGCC	6 cut(s)	58, 121, 417, 624, 850, 1043
ApaI	GGGCCC	1 cut(s)	854
ApaLI	GTGCAC	1 cut(s)	832
ApeKI	GCWGC	5 cut(s)	153, 483, 498, 686, 1328
ApoI	RAATTY	2 cut(s)	1614, 1694
AseI	ATTAAT	1 cut(s)	1667
AspLEI	GCGC	1 cut(s)	430
AspS9I	GGNCC	7 cut(s)	59, 253, 509, 625, 850, 851, 1043
AsuC2I	CCSGG	1 cut(s)	513
AsuHPI	GGTGA	2 cut(s)	19, 527
AsuII	TTCGAA	2 cut(s)	1196, 1652
AvaI	CYCGRG	2 cut(s)	1271, 1379
AvaII	GGWCC	2 cut(s)	253, 509
BaeGI	GKGCMC	2 cut(s)	836, 854
BanI	GGYRCC	1 cut(s)	211
BanII	GRGCYC	2 cut(s)	655, 854
BauI	CACGAG	1 cut(s)	1563
BbsI	GAAGAC	1 cut(s)	25
Bbv12I	GWGCWC	2 cut(s)	655, 836
BbvCI	CCTCAGC	1 cut(s)	189
BbvI	GCAGC	5 cut(s)	140, 470, 510, 673, 1315
BccI	CCATC	7 cut(s)	12, 149, 341, 362, 578, 1054, 1532
BceAI	ACGGC	1 cut(s)	108
BcgI	CGANNNNNNTGC	4 cut(s)	201, 235, 487, 521
BciT130I	CCWGG	1 cut(s)	553
BcnI	CCSGG	1 cut(s)	513
BcoDI	GTCTC	2 cut(s)	1208, 1345
BfaI	CTAG	6 cut(s)	324, 333, 495, 656, 755, 1550
BfmI	CTRYAG	2 cut(s)	687, 795
BfrI	CTTAAG	2 cut(s)	983, 1409
BfuAI	ACCTGC	2 cut(s)	14, 488
BglI	GCCNNNNNGGC	1 cut(s)	554
BglII	AGATCT	1 cut(s)	914
BisI	GCNGC	8 cut(s)	154, 210, 484, 499, 549, 687, 1329, 1416
BlsI	GCNGC	8 cut(s)	155, 211, 485, 500, 550, 688, 1330, 1417
Bme1390I	CCNGG	2 cut(s)	513, 553
Bme18I	GGWCC	2 cut(s)	253, 509
BmeT110I	CYCGRG	2 cut(s)	1271, 1379
BmgT120I	GGNCC	7 cut(s)	59, 253, 509, 625, 850, 851, 1043
BmiI	GGNNCC	6 cut(s)	61, 213, 511, 720, 852, 870
BmrFI	CCNGG	2 cut(s)	513, 553
BmsI	GCATC	4 cut(s)	97, 274, 798, 1223
BpiI	GAAGAC	1 cut(s)	25
Bpu10I	CCTNAGC	2 cut(s)	189, 396
Bpu14I	TTCGAA	2 cut(s)	1196, 1652
BpuEI	CTTGAG	1 cut(s)	1033
BpuMI	CCSGG	1 cut(s)	513
Bsa29I	ATCGAT	2 cut(s)	69, 1536
BsaAI	YACGTR	1 cut(s)	609
BsaBI	GATNNNNATC	3 cut(s)	788, 1145, 1684
BsaHI	GRCGYC	1 cut(s)	881
BsaI	GGTCTC	1 cut(s)	1345
BsaJI	CCNNGG	3 cut(s)	55, 552, 779
BsaXI	ACNNNNNCTCC	2 cut(s)	313, 343
Bsc4I	CCNNNNNNNGG	5 cut(s)	157, 208, 250, 519, 558
Bse1I	ACTGG	1 cut(s)	1434
Bse8I	GATNNNNATC	3 cut(s)	788, 1145, 1684
BseBI	CCWGG	1 cut(s)	553
BseCI	ATCGAT	2 cut(s)	69, 1536
BseDI	CCNNGG	3 cut(s)	55, 552, 779
BseGI	GGATG	2 cut(s)	379, 867
BseJI	GATNNNNATC	3 cut(s)	788, 1145, 1684
BseLI	CCNNNNNNNGG	5 cut(s)	157, 208, 250, 519, 558
BseMII	CTCAG	3 cut(s)	120, 180, 1317
BseNI	ACTGG	1 cut(s)	1434
BseSI	GKGCMC	2 cut(s)	836, 854
BseX3I	CGGCCG	1 cut(s)	121
BseXI	GCAGC	5 cut(s)	140, 470, 510, 673, 1315
BsgI	GTGCAG	1 cut(s)	663
Bsh1285I	CGRYCG	1 cut(s)	124
BshFI	GGCC	6 cut(s)	60, 123, 419, 626, 852, 1045
BshNI	GGYRCC	1 cut(s)	211
BshVI	ATCGAT	2 cut(s)	69, 1536
BsiEI	CGRYCG	1 cut(s)	124
BsiHKAI	GWGCWC	2 cut(s)	655, 836
BsiHKCI	CYCGRG	2 cut(s)	1271, 1379
BsiSI	CCGG	1 cut(s)	513
BslI	CCNNNNNNNGG	5 cut(s)	157, 208, 250, 519, 558
BsmAI	GTCTC	2 cut(s)	1208, 1345
BsnI	GGCC	6 cut(s)	60, 123, 419, 626, 852, 1045
Bso31I	GGTCTC	1 cut(s)	1345
BsoBI	CYCGRG	2 cut(s)	1271, 1379
Bsp119I	TTCGAA	2 cut(s)	1196, 1652
Bsp120I	GGGCCC	1 cut(s)	850
Bsp1286I	GDGCHC	3 cut(s)	655, 836, 854
Bsp143I	GATC	6 cut(s)	70, 160, 225, 914, 940, 1146
BspACI	CCGC	5 cut(s)	151, 209, 549, 1074, 1415
BspANI	GGCC	6 cut(s)	60, 123, 419, 626, 852, 1045
BspCNI	CTCAG	3 cut(s)	121, 181, 1316
BspDI	ATCGAT	2 cut(s)	69, 1536
BspLI	GGNNCC	6 cut(s)	61, 213, 511, 720, 852, 870
BspMAI	CTGCAG	1 cut(s)	691
BspMI	ACCTGC	2 cut(s)	14, 488
BspPI	GGATC	2 cut(s)	168, 948
BspT104I	TTCGAA	2 cut(s)	1196, 1652
BspT107I	GGYRCC	1 cut(s)	211
BspTI	CTTAAG	2 cut(s)	983, 1409
BspTNI	GGTCTC	1 cut(s)	1345
BsrI	ACTGG	1 cut(s)	1434
BssECI	CCNNGG	3 cut(s)	55, 552, 779
BssMI	GATC	6 cut(s)	70, 160, 225, 914, 940, 1146
BssNI	GRCGYC	1 cut(s)	881
BssSI	CACGAG	1 cut(s)	1563
BssT1I	CCWWGG	1 cut(s)	779
Bst2BI	CACGAG	1 cut(s)	1563
Bst2UI	CCWGG	1 cut(s)	553
Bst4CI	ACNGT	6 cut(s)	31, 461, 673, 876, 1098, 1189
Bst6I	CTCTTC	4 cut(s)	302, 432, 1193, 1302
BstACI	GRCGYC	1 cut(s)	881
BstAFI	CTTAAG	2 cut(s)	983, 1409
BstBAI	YACGTR	1 cut(s)	609
BstBI	TTCGAA	2 cut(s)	1196, 1652
BstC8I	GCNNGC	1 cut(s)	651
BstDEI	CTNAG	5 cut(s)	129, 189, 396, 1303, 1436
BstF5I	GGATG	2 cut(s)	379, 867
BstHHI	GCGC	1 cut(s)	430
BstKTI	GATC	6 cut(s)	73, 163, 228, 917, 943, 1149
BstMAI	GTCTC	2 cut(s)	1208, 1345
BstMBI	GATC	6 cut(s)	70, 160, 225, 914, 940, 1146
BstMCI	CGRYCG	1 cut(s)	124
BstMWI	GCNNNNNNNGC	9 cut(s)	333, 425, 489, 498, 554, 650, 683, 695, 1499
BstNI	CCWGG	1 cut(s)	553
BstNSI	RCATGY	2 cut(s)	180, 824
BstSCI	CCNGG	2 cut(s)	511, 551
BstSFI	CTRYAG	2 cut(s)	687, 795
BstSLI	GKGCMC	2 cut(s)	836, 854
BstV1I	GCAGC	5 cut(s)	140, 470, 510, 673, 1315
BstV2I	GAAGAC	1 cut(s)	25
BstX2I	RGATCY	1 cut(s)	914
BstYI	RGATCY	1 cut(s)	914
BstZI	CGGCCG	1 cut(s)	121
Bsu15I	ATCGAT	2 cut(s)	69, 1536
BsuRI	GGCC	6 cut(s)	60, 123, 419, 626, 852, 1045
BsuTUI	ATCGAT	2 cut(s)	69, 1536
BtgZI	GCGATG	1 cut(s)	24
BtsCI	GGATG	2 cut(s)	379, 867
BtsI	GCAGTG	1 cut(s)	1488
BtsIMutI	CAGTG	1 cut(s)	1488
BveI	ACCTGC	2 cut(s)	14, 488
Cac8I	GCNNGC	1 cut(s)	651
CfoI	GCGC	1 cut(s)	430
Cfr13I	GGNCC	7 cut(s)	59, 253, 509, 625, 850, 851, 1043
ClaI	ATCGAT	2 cut(s)	69, 1536
CseI	GACGC	3 cut(s)	13, 889, 1097
Csp6I	GTAC	1 cut(s)	133
CviQI	GTAC	1 cut(s)	133
DdeI	CTNAG	5 cut(s)	129, 189, 396, 1303, 1436
DpnI	GATC	6 cut(s)	72, 162, 227, 916, 942, 1148
DpnII	GATC	6 cut(s)	70, 160, 225, 914, 940, 1146
DraI	TTTAAA	2 cut(s)	922, 1371
DrdI	GACNNNNNNGTC	1 cut(s)	997
DseDI	GACNNNNNNGTC	1 cut(s)	997
EaeI	YGGCCR	2 cut(s)	121, 417
EagI	CGGCCG	1 cut(s)	121
Eam1104I	CTCTTC	4 cut(s)	302, 432, 1193, 1302
EarI	CTCTTC	4 cut(s)	302, 432, 1193, 1302
Ecl136II	GAGCTC	1 cut(s)	653
EclXI	CGGCCG	1 cut(s)	121
Eco130I	CCWWGG	1 cut(s)	779
Eco24I	GRGCYC	2 cut(s)	655, 854
Eco31I	GGTCTC	1 cut(s)	1345
Eco47I	GGWCC	2 cut(s)	253, 509
Eco52I	CGGCCG	1 cut(s)	121
Eco53kI	GAGCTC	1 cut(s)	653
Eco57I	CTGAAG	2 cut(s)	956, 1445
Eco88I	CYCGRG	2 cut(s)	1271, 1379
EcoICRI	GAGCTC	1 cut(s)	653
EcoRII	CCWGG	1 cut(s)	551
EcoT14I	CCWWGG	1 cut(s)	779
EcoT38I	GRGCYC	2 cut(s)	655, 854
ErhI	CCWWGG	1 cut(s)	779
FauNDI	CATATG	1 cut(s)	204
Fnu4HI	GCNGC	8 cut(s)	154, 210, 484, 499, 549, 687, 1329, 1416
FokI	GGATG	2 cut(s)	386, 874
FriOI	GRGCYC	2 cut(s)	655, 854
Fsp4HI	GCNGC	8 cut(s)	154, 210, 484, 499, 549, 687, 1329, 1416
FspBI	CTAG	6 cut(s)	324, 333, 495, 656, 755, 1550
GlaI	GCGC	1 cut(s)	429
GluI	GCNGC	8 cut(s)	154, 210, 484, 499, 549, 687, 1329, 1416
HaeIII	GGCC	6 cut(s)	60, 123, 419, 626, 852, 1045
HapII	CCGG	1 cut(s)	513
HgaI	GACGC	3 cut(s)	13, 889, 1097
HhaI	GCGC	1 cut(s)	430
Hin1I	GRCGYC	1 cut(s)	881
Hin6I	GCGC	1 cut(s)	428
HinP1I	GCGC	1 cut(s)	428
HincII	GTYRAC	1 cut(s)	879
HindII	GTYRAC	1 cut(s)	879
HindIII	AAGCTT	4 cut(s)	842, 1053, 1277, 1554
HinfI	GANTC	8 cut(s)	45, 86, 96, 998, 1166, 1201, 1569, 1601
HpaII	CCGG	1 cut(s)	513
HphI	GGTGA	2 cut(s)	19, 527
Hpy166II	GTNNAC	3 cut(s)	834, 879, 1568
Hpy188I	TCNGA	5 cut(s)	44, 85, 509, 1306, 1464
Hpy188III	TCNNGA	8 cut(s)	74, 755, 888, 974, 1273, 1381, 1550, 1662
Hpy8I	GTNNAC	3 cut(s)	834, 879, 1568
Hpy99I	CGWCG	1 cut(s)	226
HpyCH4III	ACNGT	6 cut(s)	31, 461, 673, 876, 1098, 1189
HpyCH4IV	ACGT	2 cut(s)	608, 621
HpyF10VI	GCNNNNNNNGC	9 cut(s)	333, 425, 489, 498, 554, 650, 683, 695, 1499
HpyF3I	CTNAG	5 cut(s)	129, 189, 396, 1303, 1436
HpySE526I	ACGT	2 cut(s)	608, 621
Hsp92I	GRCGYC	1 cut(s)	881
HspAI	GCGC	1 cut(s)	428
Kzo9I	GATC	6 cut(s)	70, 160, 225, 914, 940, 1146
LmnI	GCTCC	2 cut(s)	246, 1448
Lsp1109I	GCAGC	5 cut(s)	140, 470, 510, 673, 1315
LweI	GCATC	4 cut(s)	97, 274, 798, 1223
MaeI	CTAG	6 cut(s)	324, 333, 495, 656, 755, 1550
MaeII	ACGT	2 cut(s)	608, 621
MaeIII	GTNAC	4 cut(s)	25, 522, 950, 1314
MalI	GATC	6 cut(s)	72, 162, 227, 916, 942, 1148
MboI	GATC	6 cut(s)	70, 160, 225, 914, 940, 1146
MboII	GAAGA	8 cut(s)	25, 161, 319, 449, 918, 1210, 1319, 1538
MfeI	CAATTG	2 cut(s)	813, 993
MflI	RGATCY	1 cut(s)	914
MhlI	GDGCHC	3 cut(s)	655, 836, 854
MlyI	GAGTC	5 cut(s)	95, 105, 1007, 1210, 1563
MmeI	TCCRAC	2 cut(s)	114, 1426
MnlI	CCTC	8 cut(s)	65, 110, 158, 184, 303, 970, 981, 1008
MseI	TTAA	9 cut(s)	294, 465, 726, 921, 984, 1370, 1410, 1667, 1698
MslI	CAYNNNNRTG	2 cut(s)	639, 1096
MspA1I	CMGCKG	2 cut(s)	153, 800
MspCI	CTTAAG	2 cut(s)	983, 1409
MspI	CCGG	1 cut(s)	513
MspR9I	CCNGG	2 cut(s)	513, 553
MunI	CAATTG	2 cut(s)	813, 993
MvaI	CCWGG	1 cut(s)	553
MwoI	GCNNNNNNNGC	9 cut(s)	333, 425, 489, 498, 554, 650, 683, 695, 1499
NciI	CCSGG	1 cut(s)	513
NdeI	CATATG	1 cut(s)	204
NdeII	GATC	6 cut(s)	70, 160, 225, 914, 940, 1146
NlaIV	GGNNCC	6 cut(s)	61, 213, 511, 720, 852, 870
NmeAIII	GCCGAG	1 cut(s)	36
NmuCI	GTSAC	2 cut(s)	25, 1314
NspI	RCATGY	2 cut(s)	180, 824
NspV	TTCGAA	2 cut(s)	1196, 1652
PaeR7I	CTCGAG	1 cut(s)	1271
PciI	ACATGT	1 cut(s)	820
PcsI	WCGNNNNNNNCGW	1 cut(s)	81
PfeI	GAWTC	3 cut(s)	45, 1166, 1601
PkrI	GCNGC	8 cut(s)	155, 211, 485, 500, 550, 688, 1330, 1417
PleI	GAGTC	5 cut(s)	94, 104, 1006, 1209, 1563
PpsI	GAGTC	5 cut(s)	94, 104, 1006, 1209, 1563
Ppu21I	YACGTR	1 cut(s)	609
PscI	ACATGT	1 cut(s)	820
PshBI	ATTAAT	1 cut(s)	1667
PsiI	TTATAA	1 cut(s)	707
Psp124BI	GAGCTC	1 cut(s)	655
Psp6I	CCWGG	1 cut(s)	551
PspGI	CCWGG	1 cut(s)	551
PspN4I	GGNNCC	6 cut(s)	61, 213, 511, 720, 852, 870
PspOMI	GGGCCC	1 cut(s)	850
PspPI	GGNCC	7 cut(s)	59, 253, 509, 625, 850, 851, 1043
PstI	CTGCAG	1 cut(s)	691
PsuI	RGATCY	1 cut(s)	914
PvuII	CAGCTG	1 cut(s)	800
RsaI	GTAC	1 cut(s)	134
RsaNI	GTAC	1 cut(s)	133
RseI	CAYNNNNRTG	2 cut(s)	639, 1096
SacI	GAGCTC	1 cut(s)	655
SaqAI	TTAA	9 cut(s)	294, 465, 726, 921, 984, 1370, 1410, 1667, 1698
SatI	GCNGC	8 cut(s)	154, 210, 484, 499, 549, 687, 1329, 1416
Sau3AI	GATC	6 cut(s)	70, 160, 225, 914, 940, 1146
Sau96I	GGNCC	7 cut(s)	59, 253, 509, 625, 850, 851, 1043
SchI	GAGTC	5 cut(s)	95, 105, 1007, 1210, 1563
ScrFI	CCNGG	2 cut(s)	513, 553
SduI	GDGCHC	3 cut(s)	655, 836, 854
SfaNI	GCATC	4 cut(s)	97, 274, 798, 1223
SfcI	CTRYAG	2 cut(s)	687, 795
Sfr274I	CTCGAG	1 cut(s)	1271
SfuI	TTCGAA	2 cut(s)	1196, 1652
SinI	GGWCC	2 cut(s)	253, 509
SlaI	CTCGAG	1 cut(s)	1271
SmiMI	CAYNNNNRTG	2 cut(s)	639, 1096
SmlI	CTYRAG	4 cut(s)	983, 1012, 1271, 1409
SmoI	CTYRAG	4 cut(s)	983, 1012, 1271, 1409
SsiI	CCGC	5 cut(s)	151, 209, 549, 1074, 1415
SspMI	CTAG	6 cut(s)	324, 333, 495, 656, 755, 1550
SstI	GAGCTC	1 cut(s)	655
StyD4I	CCNGG	2 cut(s)	511, 551
StyI	CCWWGG	1 cut(s)	779
TaaI	ACNGT	6 cut(s)	31, 461, 673, 876, 1098, 1189
TaiI	ACGT	2 cut(s)	611, 624
TaqII	GACCGA	1 cut(s)	241
TatI	WGTACW	1 cut(s)	132
TauI	GCSGC	3 cut(s)	212, 551, 1418
TfiI	GAWTC	3 cut(s)	45, 1166, 1601
Tru1I	TTAA	9 cut(s)	294, 465, 726, 921, 984, 1370, 1410, 1667, 1698
Tru9I	TTAA	9 cut(s)	294, 465, 726, 921, 984, 1370, 1410, 1667, 1698
TscAI	CASTG	1 cut(s)	1495
TseFI	GTSAC	2 cut(s)	25, 1314
TseI	GCWGC	5 cut(s)	153, 483, 498, 686, 1328
Tsp45I	GTSAC	2 cut(s)	25, 1314
TspGWI	ACGGA	2 cut(s)	108, 522
TspRI	CASTG	1 cut(s)	1495
Vha464I	CTTAAG	2 cut(s)	983, 1409
VneI	GTGCAC	1 cut(s)	832
VpaK11BI	GGWCC	2 cut(s)	253, 509
VspI	ATTAAT	1 cut(s)	1667
XapI	RAATTY	2 cut(s)	1614, 1694
XbaI	TCTAGA	2 cut(s)	754, 1549
XceI	RCATGY	2 cut(s)	180, 824
XhoI	CTCGAG	1 cut(s)	1271
XspI	CTAG	6 cut(s)	324, 333, 495, 656, 755, 1550

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.