Rh4AG141600

3beta-hydroxysteroid-dehydrogenase decarboxylase isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003789
Physical Location & Seq
Forward (+)
35942077 .. 35949515
7439 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG141600.1

Sequence Viewer

Length: 1770 bp
ATGAATTTGACCCCTCCACAGCCTTCAAGGAATAAGGAATTGGAGACTTCACCGATGACCTGCGATGAGAGATGGTGCGTCGTCACCGGAGGCCGAGGCTTTGCTGCTCGCCATTTGGTTCTCATGCTCATCCGCCAGAACGTCTTCCATGTCCGAATCGCTGACCTCGGCCCCACCATCGATCTCGAACCTTCCGAGTCCAACGGAGTCCTCGCCGATGCTTTGAACTTCGGCCGTGCTGAGTACATTTCCATCGACCTCCGCTGCAAGGATCAAGTTCTTCAAGCATGTCAAGGTGCTGAGGTTGTGTTCCATATGGCGGCACCTAATTCGTCGATCAACAATTACCAGCTGCACCATTCGGTCAATGTGGAAGGAACTAAAAATGTGATTGATGCTTGTGTTAAGCAGAAAGTGAAGAGGCTCATATACACTAGCTCTCCTAGCGTTGTGTTTGATGGGGTTCATGGTATTATTGATGGGGATGAGTTGTTGTCATATCCACCTAAGCACAATGATACTTATTCGGCCACTAAAGCGCAAGGAGAAGAGTTGGTAATCAAGGCAAACGGTGTTAAAGGGCTTCTAACCTGCTGCATACGCCCTAGCAGCATTTTCGGACCCGGTGATAGGTTACTTGTTCCGTCTTTAGTTGTTGCCGCCAGGGCAGGGAAATCCAAGTTCATTATAGGAGATGGCAATAACATTTATGATTTCACGTATGTTGAAAACGTGGCCCATGCCCATATATGTGCAGAGCGAGCTCTAGCATTTGAAGGCACAGTTGCAGAGAAAGCTGCAGGACAGTCGCCTTCGCCTTCGCTTTCGCATTTGAAAACACTGTATTTTATAACCAATATGGAACCTATTAAGTTTTGGGAGTTTATTTCACTTATTCTAGAAGGTCTTGGTTATGAAAGACCAAGGATAAAAATCCCTACAGCTGTTATGATGCCAATTGCACATGTGGTGGAGTGCACATATAAGCTTTTGGGCCCGTATGGGATGAAGGTTCCACAGTTGACGCCTTCAAGAATTAGACTTCTCTCTTGCACCAGATCTTTTAAATCTTCAAAAGCACAGGATCGAATTGGTTACACACCCATTATTTCGCTTCAGGAGGGTCTTAAGAGGACAATTGAGTCATACCCACACCTGAGGGCTGAACATCAACCCAAAAGAGAAGGGCCATCTAAAGCTTCTGTATATCTTGGAAGCGGAAGGGTTGCTGACGCATTACTGTGGAAGGATAAAAAACAGACATTCAAGGCACTACTAGTTTTGATTGCGATCTACTACAACTTCATTGAATCTAAAGCTACGATTATTACTGTCATTTCGAAGAGTCTCATGTTCGCATCAATTTTCATGTTCATCTCTGGCAATTTACCAGAAATGATATTAGGATATAAACTCGAGAAGCTTCAATCATCACATTTTCACTTCTCAGAAGAGATGTCACAACAAATTGCTGCATTTGTGGTCTCATCATGGAATATATCTGTCAAAACTTTTAAATCTCTCGGGAAAGGAAATGACTGCATATTGTTTCTTAAGGCGGCTGTATCTCTACCGGTTCTTAGCTGTGTTGGAGCTATTTCACTTCAGAACTCATTTATTATAGGAGTTTCCACTGCCTTTTTAGCCTTCTTTGTGTATGAAAAGCAGGAAGAAAAAATCGATGGCATGGTTCTAGAAGCTTTCTCTCGTGGACGCAAATTGAAGTCCAATGTACTTCGAAAATTCAGTACATCCAAAAGGATTGAGTGA

Protein Analysis

589

Amino Acids

65.23

Weight (kDa)

9.13

Isoelectric Point (pI)

43.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
3Beta_HSD PF01073 27 - 312 9.2e-72 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 27 - 253 3.6e-33 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 27 - 147 8.8e-07 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 28 - 249 5.3e-12 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 78 - 151 2.4e-07 NAD(P)H-binding
NAD_binding_4 PF07993 95 - 246 8.7e-14 Male sterility protein
Reticulon PF02453 409 - 564 2.8e-29 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 851
AasI GACNNNNNNGTC 1 cut(s) 1141
Acc36I ACCTGC 2 cut(s) 68, 599
AccB1I GGYRCC 1 cut(s) 322
AciI CCGC 6 cut(s) 133, 262, 320, 660, 1218, 1559
AclWI GGATC 2 cut(s) 279, 1092
AcoI YGGCCR 2 cut(s) 232, 528
AcsI RAATTY 2 cut(s) 4, 1742
AcuI CTGAAG 2 cut(s) 1100, 1589
AcyI GRCGYC 1 cut(s) 1025
AfaI GTAC 3 cut(s) 245, 1734, 1750
AfiI CCNNNNNNNGG 4 cut(s) 268, 319, 630, 669
AflII CTTAAG 2 cut(s) 1127, 1553
AflIII ACRYGT 1 cut(s) 964
AgeI ACCGGT 1 cut(s) 1573
AhlI ACTAGT 1 cut(s) 1276
AjnI CCWGG 1 cut(s) 662
AjuI GAANNNNNNNTTGG 2 cut(s) 23, 55
Alw21I GWGCWC 2 cut(s) 766, 980
Alw26I GTCTC 3 cut(s) 38, 1352, 1489
Alw44I GTGCAC 1 cut(s) 976
AlwI GGATC 2 cut(s) 279, 1092
Ama87I CYCGRG 2 cut(s) 1415, 1523
AoxI GGCC 7 cut(s) 91, 169, 232, 528, 735, 994, 1187
ApaI GGGCCC 1 cut(s) 998
ApaLI GTGCAC 1 cut(s) 976
ApeKI GCWGC 7 cut(s) 104, 264, 352, 594, 609, 797, 1472
ApoI RAATTY 2 cut(s) 4, 1742
AsiGI ACCGGT 1 cut(s) 1573
Asp700I GAANNNNTTC 1 cut(s) 143
AspLEI GCGC 1 cut(s) 541
AspS9I GGNCC 6 cut(s) 170, 620, 736, 994, 995, 1187
AsuC2I CCSGG 1 cut(s) 624
AsuHPI GGTGA 3 cut(s) 42, 76, 638
AsuII TTCGAA 2 cut(s) 1340, 1738
AvaI CYCGRG 2 cut(s) 1415, 1523
AvaII GGWCC 1 cut(s) 620
AxyI CCTNAGG 1 cut(s) 1157
BaeGI GKGCMC 2 cut(s) 980, 998
BanI GGYRCC 1 cut(s) 322
BanII GRGCYC 2 cut(s) 766, 998
BauI CACGAG 1 cut(s) 1707
BbsI GAAGAC 1 cut(s) 136
Bbv12I GWGCWC 2 cut(s) 766, 980
BbvCI CCTCAGC 1 cut(s) 300
BbvI GCAGC 7 cut(s) 91, 251, 339, 581, 621, 784, 1459
BccI CCATC 8 cut(s) 66, 185, 260, 452, 473, 689, 1198, 1676
BceAI ACGGC 1 cut(s) 219
BcgI CGANNNNNNTGC 6 cut(s) 312, 346, 598, 632, 789, 823
BciT130I CCWGG 1 cut(s) 664
BcnI CCSGG 1 cut(s) 624
BcoDI GTCTC 3 cut(s) 38, 1352, 1489
BcuI ACTAGT 1 cut(s) 1276
BfaI CTAG 7 cut(s) 435, 444, 606, 767, 899, 1277, 1694
BfmI CTRYAG 2 cut(s) 798, 939
BfrI CTTAAG 2 cut(s) 1127, 1553
BfuAI ACCTGC 2 cut(s) 68, 599
BglI GCCNNNNNGGC 1 cut(s) 665
BglII AGATCT 1 cut(s) 1058
Bme1390I CCNGG 2 cut(s) 624, 664
Bme18I GGWCC 1 cut(s) 620
BmeT110I CYCGRG 2 cut(s) 1415, 1523
BmgT120I GGNCC 6 cut(s) 170, 620, 736, 994, 995, 1187
BmiI GGNNCC 6 cut(s) 172, 324, 622, 864, 996, 1014
BmrFI CCNGG 2 cut(s) 624, 664
BmsI GCATC 4 cut(s) 208, 385, 942, 1367
BpiI GAAGAC 1 cut(s) 136
Bpu10I CCTNAGC 2 cut(s) 300, 507
Bpu14I TTCGAA 2 cut(s) 1340, 1738
BpuMI CCSGG 1 cut(s) 624
Bsa29I ATCGAT 2 cut(s) 180, 1680
BsaAI YACGTR 1 cut(s) 720
BsaBI GATNNNNATC 2 cut(s) 932, 1289
BsaHI GRCGYC 1 cut(s) 1025
BsaI GGTCTC 1 cut(s) 1489
BsaJI CCNNGG 4 cut(s) 94, 166, 663, 923
BsaWI WCCGGW 2 cut(s) 86, 1573
BsaXI ACNNNNNCTCC 2 cut(s) 424, 454
Bsc4I CCNNNNNNNGG 4 cut(s) 268, 319, 630, 669
Bse118I RCCGGY 1 cut(s) 1573
Bse21I CCTNAGG 1 cut(s) 1157
Bse8I GATNNNNATC 2 cut(s) 932, 1289
BseBI CCWGG 1 cut(s) 664
BseCI ATCGAT 2 cut(s) 180, 1680
BseDI CCNNGG 4 cut(s) 94, 166, 663, 923
BseGI GGATG 4 cut(s) 129, 490, 1011, 1751
BseJI GATNNNNATC 2 cut(s) 932, 1289
BseLI CCNNNNNNNGG 4 cut(s) 268, 319, 630, 669
BseMII CTCAG 4 cut(s) 231, 291, 1148, 1461
BseSI GKGCMC 2 cut(s) 980, 998
BseX3I CGGCCG 1 cut(s) 232
BseXI GCAGC 7 cut(s) 91, 251, 339, 581, 621, 784, 1459
BsgI GTGCAG 2 cut(s) 338, 774
Bsh1285I CGRYCG 1 cut(s) 235
BshFI GGCC 7 cut(s) 93, 171, 234, 530, 737, 996, 1189
BshNI GGYRCC 1 cut(s) 322
BshTI ACCGGT 1 cut(s) 1573
BshVI ATCGAT 2 cut(s) 180, 1680
BsiEI CGRYCG 1 cut(s) 235
BsiHKAI GWGCWC 2 cut(s) 766, 980
BsiHKCI CYCGRG 2 cut(s) 1415, 1523
BsiSI CCGG 3 cut(s) 87, 624, 1574
BslI CCNNNNNNNGG 4 cut(s) 268, 319, 630, 669
BsmAI GTCTC 3 cut(s) 38, 1352, 1489
BsnI GGCC 7 cut(s) 93, 171, 234, 530, 737, 996, 1189
Bso31I GGTCTC 1 cut(s) 1489
BsoBI CYCGRG 2 cut(s) 1415, 1523
Bsp119I TTCGAA 2 cut(s) 1340, 1738
Bsp120I GGGCCC 1 cut(s) 994
Bsp1286I GDGCHC 3 cut(s) 766, 980, 998
Bsp143I GATC 6 cut(s) 181, 271, 336, 1058, 1084, 1290
BspACI CCGC 6 cut(s) 133, 262, 320, 660, 1218, 1559
BspANI GGCC 7 cut(s) 93, 171, 234, 530, 737, 996, 1189
BspCNI CTCAG 4 cut(s) 232, 292, 1149, 1460
BspDI ATCGAT 2 cut(s) 180, 1680
BspLI GGNNCC 6 cut(s) 172, 324, 622, 864, 996, 1014
BspMAI CTGCAG 1 cut(s) 802
BspMI ACCTGC 2 cut(s) 68, 599
BspPI GGATC 2 cut(s) 279, 1092
BspT104I TTCGAA 2 cut(s) 1340, 1738
BspT107I GGYRCC 1 cut(s) 322
BspTI CTTAAG 2 cut(s) 1127, 1553
BspTNI GGTCTC 1 cut(s) 1489
BsrFI RCCGGY 1 cut(s) 1573
BssAI RCCGGY 1 cut(s) 1573
BssECI CCNNGG 4 cut(s) 94, 166, 663, 923
BssMI GATC 6 cut(s) 181, 271, 336, 1058, 1084, 1290
BssNI GRCGYC 1 cut(s) 1025
BssSI CACGAG 1 cut(s) 1707
BssT1I CCWWGG 1 cut(s) 923
Bst2BI CACGAG 1 cut(s) 1707
Bst2UI CCWGG 1 cut(s) 664
Bst4CI ACNGT 7 cut(s) 572, 784, 807, 843, 1020, 1242, 1333
Bst6I CTCTTC 4 cut(s) 413, 543, 1337, 1446
BstACI GRCGYC 1 cut(s) 1025
BstAFI CTTAAG 2 cut(s) 1127, 1553
BstBAI YACGTR 1 cut(s) 720
BstBI TTCGAA 2 cut(s) 1340, 1738
BstC8I GCNNGC 2 cut(s) 109, 762
BstDEI CTNAG 6 cut(s) 240, 300, 507, 1157, 1447, 1580
BstF5I GGATG 4 cut(s) 129, 490, 1011, 1751
BstHHI GCGC 1 cut(s) 541
BstKTI GATC 6 cut(s) 184, 274, 339, 1061, 1087, 1293
BstMAI GTCTC 3 cut(s) 38, 1352, 1489
BstMBI GATC 6 cut(s) 181, 271, 336, 1058, 1084, 1290
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 8 cut(s) 444, 536, 600, 609, 665, 761, 794, 1643
BstNI CCWGG 1 cut(s) 664
BstNSI RCATGY 2 cut(s) 291, 968
BstSCI CCNGG 2 cut(s) 622, 662
BstSFI CTRYAG 2 cut(s) 798, 939
BstSLI GKGCMC 2 cut(s) 980, 998
BstV1I GCAGC 7 cut(s) 91, 251, 339, 581, 621, 784, 1459
BstV2I GAAGAC 1 cut(s) 136
BstX2I RGATCY 1 cut(s) 1058
BstYI RGATCY 1 cut(s) 1058
BstZI CGGCCG 1 cut(s) 232
Bsu15I ATCGAT 2 cut(s) 180, 1680
Bsu36I CCTNAGG 1 cut(s) 1157
BsuRI GGCC 7 cut(s) 93, 171, 234, 530, 737, 996, 1189
BsuTUI ATCGAT 2 cut(s) 180, 1680
BtgZI GCGATG 1 cut(s) 78
BtsCI GGATG 4 cut(s) 129, 490, 1011, 1751
BtsI GCAGTG 1 cut(s) 1632
BtsIMutI CAGTG 2 cut(s) 839, 1632
BveI ACCTGC 2 cut(s) 68, 599
Cac8I GCNNGC 2 cut(s) 109, 762
CfoI GCGC 1 cut(s) 541
Cfr10I RCCGGY 1 cut(s) 1573
Cfr13I GGNCC 6 cut(s) 170, 620, 736, 994, 995, 1187
ClaI ATCGAT 2 cut(s) 180, 1680
CseI GACGC 4 cut(s) 67, 1033, 1241, 1722
Csp6I GTAC 3 cut(s) 244, 1733, 1749
CspAI ACCGGT 1 cut(s) 1573
CviQI GTAC 3 cut(s) 244, 1733, 1749
DdeI CTNAG 6 cut(s) 240, 300, 507, 1157, 1447, 1580
DpnI GATC 6 cut(s) 183, 273, 338, 1060, 1086, 1292
DpnII GATC 6 cut(s) 181, 271, 336, 1058, 1084, 1290
DraI TTTAAA 2 cut(s) 1066, 1516
DrdI GACNNNNNNGTC 1 cut(s) 1141
DseDI GACNNNNNNGTC 1 cut(s) 1141
EaeI YGGCCR 2 cut(s) 232, 528
EagI CGGCCG 1 cut(s) 232
Eam1104I CTCTTC 4 cut(s) 413, 543, 1337, 1446
EarI CTCTTC 4 cut(s) 413, 543, 1337, 1446
EciI GGCGGA 1 cut(s) 122
Ecl136II GAGCTC 1 cut(s) 764
EclXI CGGCCG 1 cut(s) 232
Eco130I CCWWGG 1 cut(s) 923
Eco24I GRGCYC 2 cut(s) 766, 998
Eco31I GGTCTC 1 cut(s) 1489
Eco47I GGWCC 1 cut(s) 620
Eco52I CGGCCG 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 764
Eco57I CTGAAG 2 cut(s) 1100, 1589
Eco81I CCTNAGG 1 cut(s) 1157
Eco88I CYCGRG 2 cut(s) 1415, 1523
EcoICRI GAGCTC 1 cut(s) 764
EcoRII CCWGG 1 cut(s) 662
EcoT14I CCWWGG 1 cut(s) 923
EcoT38I GRGCYC 2 cut(s) 766, 998
ErhI CCWWGG 1 cut(s) 923
FauNDI CATATG 1 cut(s) 315
FokI GGATG 4 cut(s) 116, 497, 1018, 1738
FriOI GRGCYC 2 cut(s) 766, 998
FspBI CTAG 7 cut(s) 435, 444, 606, 767, 899, 1277, 1694
GlaI GCGC 1 cut(s) 540
HaeIII GGCC 7 cut(s) 93, 171, 234, 530, 737, 996, 1189
HapII CCGG 3 cut(s) 87, 624, 1574
HgaI GACGC 4 cut(s) 67, 1033, 1241, 1722
HhaI GCGC 1 cut(s) 541
Hin1I GRCGYC 1 cut(s) 1025
Hin6I GCGC 1 cut(s) 539
HinP1I GCGC 1 cut(s) 539
HincII GTYRAC 1 cut(s) 1023
HindII GTYRAC 1 cut(s) 1023
HindIII AAGCTT 4 cut(s) 986, 1197, 1421, 1698
HinfI GANTC 6 cut(s) 156, 197, 207, 1142, 1310, 1345
HpaII CCGG 3 cut(s) 87, 624, 1574
HphI GGTGA 3 cut(s) 42, 76, 638
Hpy166II GTNNAC 3 cut(s) 978, 1023, 1712
Hpy188I TCNGA 5 cut(s) 155, 196, 620, 1450, 1608
Hpy188III TCNNGA 7 cut(s) 185, 899, 1032, 1118, 1417, 1525, 1694
Hpy8I GTNNAC 3 cut(s) 978, 1023, 1712
Hpy99I CGWCG 2 cut(s) 83, 337
HpyCH4III ACNGT 7 cut(s) 572, 784, 807, 843, 1020, 1242, 1333
HpyCH4IV ACGT 3 cut(s) 141, 719, 732
HpyF10VI GCNNNNNNNGC 8 cut(s) 444, 536, 600, 609, 665, 761, 794, 1643
HpyF3I CTNAG 6 cut(s) 240, 300, 507, 1157, 1447, 1580
HpySE526I ACGT 3 cut(s) 141, 719, 732
Hsp92I GRCGYC 1 cut(s) 1025
HspAI GCGC 1 cut(s) 539
Kzo9I GATC 6 cut(s) 181, 271, 336, 1058, 1084, 1290
LmnI GCTCC 1 cut(s) 1592
Lsp1109I GCAGC 7 cut(s) 91, 251, 339, 581, 621, 784, 1459
LweI GCATC 4 cut(s) 208, 385, 942, 1367
MaeI CTAG 7 cut(s) 435, 444, 606, 767, 899, 1277, 1694
MaeII ACGT 3 cut(s) 141, 719, 732
MaeIII GTNAC 4 cut(s) 82, 633, 1094, 1458
MalI GATC 6 cut(s) 183, 273, 338, 1060, 1086, 1292
MboI GATC 6 cut(s) 181, 271, 336, 1058, 1084, 1290
MboII GAAGA 8 cut(s) 136, 272, 430, 560, 1062, 1354, 1463, 1682
MfeI CAATTG 2 cut(s) 957, 1137
MflI RGATCY 1 cut(s) 1058
MhlI GDGCHC 3 cut(s) 766, 980, 998
MlyI GAGTC 4 cut(s) 206, 216, 1151, 1354
MmeI TCCRAC 2 cut(s) 225, 1570
MroXI GAANNNNTTC 1 cut(s) 143
MseI TTAA 7 cut(s) 405, 576, 870, 1065, 1128, 1515, 1554
MslI CAYNNNNRTG 2 cut(s) 750, 1240
MspA1I CMGCKG 3 cut(s) 264, 352, 944
MspCI CTTAAG 2 cut(s) 1127, 1553
MspI CCGG 3 cut(s) 87, 624, 1574
MspR9I CCNGG 2 cut(s) 624, 664
MunI CAATTG 2 cut(s) 957, 1137
MvaI CCWGG 1 cut(s) 664
MwoI GCNNNNNNNGC 8 cut(s) 444, 536, 600, 609, 665, 761, 794, 1643
NciI CCSGG 1 cut(s) 624
NdeI CATATG 1 cut(s) 315
NdeII GATC 6 cut(s) 181, 271, 336, 1058, 1084, 1290
NlaIV GGNNCC 6 cut(s) 172, 324, 622, 864, 996, 1014
NmeAIII GCCGAG 2 cut(s) 119, 147
NmuCI GTSAC 2 cut(s) 82, 1458
NspI RCATGY 2 cut(s) 291, 968
NspV TTCGAA 2 cut(s) 1340, 1738
PaeR7I CTCGAG 1 cut(s) 1415
PciI ACATGT 1 cut(s) 964
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PdmI GAANNNNTTC 1 cut(s) 143
PfeI GAWTC 2 cut(s) 156, 1310
PinAI ACCGGT 1 cut(s) 1573
PleI GAGTC 4 cut(s) 205, 215, 1150, 1353
PpsI GAGTC 4 cut(s) 205, 215, 1150, 1353
Ppu21I YACGTR 1 cut(s) 720
PscI ACATGT 1 cut(s) 964
PsiI TTATAA 1 cut(s) 851
Psp124BI GAGCTC 1 cut(s) 766
Psp6I CCWGG 1 cut(s) 662
PspGI CCWGG 1 cut(s) 662
PspN4I GGNNCC 6 cut(s) 172, 324, 622, 864, 996, 1014
PspOMI GGGCCC 1 cut(s) 994
PspPI GGNCC 6 cut(s) 170, 620, 736, 994, 995, 1187
PstI CTGCAG 1 cut(s) 802
PsuI RGATCY 1 cut(s) 1058
PvuII CAGCTG 2 cut(s) 352, 944
RsaI GTAC 3 cut(s) 245, 1734, 1750
RsaNI GTAC 3 cut(s) 244, 1733, 1749
RseI CAYNNNNRTG 2 cut(s) 750, 1240
SacI GAGCTC 1 cut(s) 766
SaqAI TTAA 7 cut(s) 405, 576, 870, 1065, 1128, 1515, 1554
Sau3AI GATC 6 cut(s) 181, 271, 336, 1058, 1084, 1290
Sau96I GGNCC 6 cut(s) 170, 620, 736, 994, 995, 1187
SchI GAGTC 4 cut(s) 206, 216, 1151, 1354
ScrFI CCNGG 2 cut(s) 624, 664
SduI GDGCHC 3 cut(s) 766, 980, 998
SfaNI GCATC 4 cut(s) 208, 385, 942, 1367
SfcI CTRYAG 2 cut(s) 798, 939
Sfr274I CTCGAG 1 cut(s) 1415
SfuI TTCGAA 2 cut(s) 1340, 1738
SinI GGWCC 1 cut(s) 620
SlaI CTCGAG 1 cut(s) 1415
SmiMI CAYNNNNRTG 2 cut(s) 750, 1240
SmlI CTYRAG 3 cut(s) 1127, 1415, 1553
SmoI CTYRAG 3 cut(s) 1127, 1415, 1553
SpeI ACTAGT 1 cut(s) 1276
SsiI CCGC 6 cut(s) 133, 262, 320, 660, 1218, 1559
SspMI CTAG 7 cut(s) 435, 444, 606, 767, 899, 1277, 1694
SstI GAGCTC 1 cut(s) 766
StyD4I CCNGG 2 cut(s) 622, 662
StyI CCWWGG 1 cut(s) 923
TaaI ACNGT 7 cut(s) 572, 784, 807, 843, 1020, 1242, 1333
TaiI ACGT 3 cut(s) 144, 722, 735
TaqI TCGA 9 cut(s) 180, 186, 255, 335, 1087, 1340, 1416, 1680, 1738
TaqII GACCGA 1 cut(s) 352
TatI WGTACW 3 cut(s) 243, 1732, 1748
TauI GCSGC 3 cut(s) 323, 662, 1562
TfiI GAWTC 2 cut(s) 156, 1310
Tru1I TTAA 7 cut(s) 405, 576, 870, 1065, 1128, 1515, 1554
Tru9I TTAA 7 cut(s) 405, 576, 870, 1065, 1128, 1515, 1554
TscAI CASTG 2 cut(s) 846, 1639
TseFI GTSAC 2 cut(s) 82, 1458
TseI GCWGC 7 cut(s) 104, 264, 352, 594, 609, 797, 1472
Tsp45I GTSAC 2 cut(s) 82, 1458
TspDTI ATGAA 9 cut(s) 17, 455, 673, 930, 1022, 1294, 1357, 1363, 1674
TspGWI ACGGA 2 cut(s) 219, 633
TspRI CASTG 2 cut(s) 846, 1639
Vha464I CTTAAG 2 cut(s) 1127, 1553
VneI GTGCAC 1 cut(s) 976
VpaK11BI GGWCC 1 cut(s) 620
XapI RAATTY 2 cut(s) 4, 1742
XbaI TCTAGA 2 cut(s) 898, 1693
XceI RCATGY 2 cut(s) 291, 968
XhoI CTCGAG 1 cut(s) 1415
XmnI GAANNNNTTC 1 cut(s) 143
XspI CTAG 7 cut(s) 435, 444, 606, 767, 899, 1277, 1694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.