Rh6BG455100

3beta-hydroxysteroid-dehydrogenase decarboxylase isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0003789
Physical Location & Seq
Reverse (-)
66798387 .. 66802666
4280 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG455100.1

Sequence Viewer

Length: 1683 bp
ATGGCCGGCGATGAGAGATGGTGCGTCGTCACCGGAGGCCGTGGCTTCGCCGCTCGCCATTTGGTTCTCATGCTCATCCGCCAAAACGTCTTCCGCGTCCGAATCGCCGACCTCGGCCCTACCGTCGATCTCGAACCTTCCGAGTCCAACGGAGTCCTCGCCGATGCTTTGAACTCCGGCCGCGCCCAGTACATTTCCATCGACCTCCGCCGTAAGGATCAAGTTCTTCAAGCATGTCAAGGTGCTGAGGTTGTGTTCCACATGGCGGCTCCCAATTCGTCCATCAACAATTACCAGCTCCACCATTCGGTCAATGTGGAAGGAACTAAGAATGTGATTGATGCTTGTGTTGAGCAGAAAGTGAAGAGGCTCATATACACTAGCTCTCCTAGTGTTGTGTTTGATGGGGTTCATGGCATTATTGATGGGGATGAGTCTTTGCCATATCCACCTAAGCACAATGATACTTATTCGGCCACTAAAGCGCAAGGAGAAGAGTTGGTAATCAAGGCAAACGGTGTTAAAGGGCTTCTAACCTGCTGCATACGCCCTAGCAGCATTTTCGGACCCGGTGATAGGTTACTTGTTCCATCTTTAATTGTTGCCGCCAGGGCAGGGAAATCCAAGTTCATTATCGGAGATGGCAATAACATTTATGACTTCACGTATGTTGAAAACGTGGCCCATGCCCATATATGTGCAGAACGAGCTCTAGCGTCTGAAGGGACAGTTGCAGAGAAAGCTGCAGGACAGGCATATTTTATAACCAATATGGAACCTATCAAGTTTTGGGAGTTTATGTCACTTATTCTAGAAGGTCTTGGCTATGAAAGACCAAGGATAAAAATCCCTGCAGCTGTTATGATGCCAATTGCACATGTGGTGGAGTGGACATTTAAGCTTTTTGGTCCATATGGGATGAAGGTTCCACAGTTGACGCCTTCAAGAATTAGACTTCTCTCTTGCACCAGATCTTTTAAATCTTCAAAAGCACAGGATCGAATTGGTTACACGCCCATCATTTCGCTTCAGGAGGGTCTTAAGAGGACAATCGAGTCATACCCACACTTGAGGGCTGAACATCGACCCAAAAGAGAAGGGCCATCTAAAGCTTCTGTGTATCTTGGAAGTGGGAGGGTTGCTGATGCATTACTGTGGAAGGACAAAAAACAGACATTCAAGGCACTATTATTTTTTATTGCAATCTACTACAACTTCATTGCATCTCAAGCTACAGTTATTACCGTAATTTCAAAGAGTCTCATGTTCACATCAATTTTCGTGTTCATCTCTGGCAATTTACCAGAAAAGATATTAGGATATAAACTCGAGAAGCTTCAGTCATCACATTTTAACTTATCAGAAGAGATGTCACAACAAATTGCTGGGTCGGTGGCCTCATCATGGAATATATCTGTGAAAACTTTAAAATCTCTTGGGAAAGGAAATGACTGGATATTGTTTCTTAAGGTGGCTCTATCCTTACTGGTTCTTAGCTTCCTTGGAGCCATTTCACTTCAGCACTCATTTATTATAGGAGTTTCCACTGCCTTTCTAGCCTTCTATGTGTACGAAAAGCAGGAAGAAAAAATCGATGCCATGGTCCTAGAAGCTTTCTCTCGTGGATGCAAATTGAAGTCCAATATGCTTCGAAAATTCAGTACATCCAAAAAGATTGAGTGA

Protein Analysis

560

Amino Acids

62.02

Weight (kDa)

9.31

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
3Beta_HSD PF01073 9 - 283 2.6e-78 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 9 - 237 3.1e-34 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 9 - 129 1.6e-07 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 10 - 236 1.5e-14 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 57 - 133 2.4e-08 NAD(P)H-binding
RmlD_sub_bind PF04321 66 - 282 6.1e-08 RmlD substrate binding domain
NAD_binding_4 PF07993 76 - 229 1.4e-13 Male sterility protein
Reticulon PF02453 380 - 535 2e-31 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 764
AasI GACNNNNNNGTC 1 cut(s) 1054
Acc36I ACCTGC 1 cut(s) 545
AccBSI CCGCTC 1 cut(s) 53
AccII CGCG 2 cut(s) 96, 183
AciI CCGC 7 cut(s) 51, 79, 94, 181, 208, 266, 606
AclWI GGATC 2 cut(s) 225, 1005
AcoI YGGCCR 3 cut(s) 3, 178, 474
AcsI RAATTY 1 cut(s) 1655
AcuI CTGAAG 4 cut(s) 741, 1013, 1322, 1502
AcyI GRCGYC 1 cut(s) 938
AfaI GTAC 3 cut(s) 191, 1571, 1663
AfiI CCNNNNNNNGG 6 cut(s) 214, 265, 307, 576, 615, 1404
AflII CTTAAG 2 cut(s) 1040, 1466
AflIII ACRYGT 1 cut(s) 877
AgsI TTSAA 8 cut(s) 172, 230, 674, 945, 987, 1180, 1254, 1636
AjnI CCWGG 1 cut(s) 608
Alw21I GWGCWC 1 cut(s) 712
Alw26I GTCTC 1 cut(s) 1265
AlwI GGATC 2 cut(s) 225, 1005
Ama87I CYCGRG 1 cut(s) 1328
AoxI GGCC 8 cut(s) 3, 37, 115, 178, 474, 681, 1100, 1395
ApeKI GCWGC 4 cut(s) 540, 555, 743, 854
ApoI RAATTY 1 cut(s) 1655
AspLEI GCGC 2 cut(s) 185, 487
AspS9I GGNCC 6 cut(s) 116, 566, 682, 908, 1100, 1603
AsuC2I CCSGG 1 cut(s) 570
AsuHPI GGTGA 2 cut(s) 22, 584
AsuII TTCGAA 1 cut(s) 1651
AvaI CYCGRG 1 cut(s) 1328
AvaII GGWCC 3 cut(s) 566, 908, 1603
BanII GRGCYC 1 cut(s) 712
BauI CACGAG 1 cut(s) 1620
BbsI GAAGAC 1 cut(s) 82
Bbv12I GWGCWC 1 cut(s) 712
BbvCI CCTCAGC 1 cut(s) 246
BbvI GCAGC 4 cut(s) 527, 567, 730, 866
BccI CCATC 9 cut(s) 12, 206, 290, 398, 419, 598, 635, 1025, 1111
BceAI ACGGC 2 cut(s) 24, 195
BcgI CGANNNNNNTGC 2 cut(s) 544, 578
BciT130I CCWGG 1 cut(s) 610
BcnI CCSGG 1 cut(s) 570
BcoDI GTCTC 1 cut(s) 1265
BfaI CTAG 7 cut(s) 381, 390, 552, 713, 812, 1556, 1607
BfmI CTRYAG 3 cut(s) 744, 852, 1233
BfrI CTTAAG 2 cut(s) 1040, 1466
BfuAI ACCTGC 1 cut(s) 545
BglI GCCNNNNNGGC 1 cut(s) 611
BglII AGATCT 1 cut(s) 971
BisI GCNGC 8 cut(s) 51, 181, 267, 541, 556, 606, 744, 855
BlsI GCNGC 8 cut(s) 52, 182, 268, 542, 557, 607, 745, 856
Bme1390I CCNGG 2 cut(s) 570, 610
Bme18I GGWCC 3 cut(s) 566, 908, 1603
BmeT110I CYCGRG 1 cut(s) 1328
BmgT120I GGNCC 6 cut(s) 116, 566, 682, 908, 1100, 1603
BmiI GGNNCC 5 cut(s) 270, 568, 777, 927, 1507
BmrFI CCNGG 2 cut(s) 570, 610
BmrI ACTGGG 1 cut(s) 181
BmsI GCATC 7 cut(s) 154, 331, 855, 1135, 1232, 1585, 1616
BmuI ACTGGG 1 cut(s) 181
BpiI GAAGAC 1 cut(s) 82
Bpu10I CCTNAGC 2 cut(s) 246, 453
Bpu14I TTCGAA 1 cut(s) 1651
BpuEI CTTGAG 2 cut(s) 1090, 1212
BpuMI CCSGG 1 cut(s) 570
Bsa29I ATCGAT 1 cut(s) 1593
BsaAI YACGTR 1 cut(s) 666
BsaBI GATNNNNATC 1 cut(s) 845
BsaHI GRCGYC 1 cut(s) 938
BsaJI CCNNGG 6 cut(s) 40, 112, 609, 836, 1501, 1599
BsaWI WCCGGW 1 cut(s) 32
BsaXI ACNNNNNCTCC 4 cut(s) 370, 400, 785, 815
Bsc4I CCNNNNNNNGG 6 cut(s) 214, 265, 307, 576, 615, 1404
Bse118I RCCGGY 1 cut(s) 5
Bse1I ACTGG 3 cut(s) 187, 1457, 1491
Bse3DI GCAATG 1 cut(s) 1218
Bse8I GATNNNNATC 1 cut(s) 845
BseBI CCWGG 1 cut(s) 610
BseCI ATCGAT 1 cut(s) 1593
BseDI CCNNGG 6 cut(s) 40, 112, 609, 836, 1501, 1599
BseGI GGATG 5 cut(s) 75, 436, 924, 1631, 1664
BseJI GATNNNNATC 1 cut(s) 845
BseLI CCNNNNNNNGG 6 cut(s) 214, 265, 307, 576, 615, 1404
BseMI GCAATG 1 cut(s) 1218
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 3 cut(s) 187, 1457, 1491
BseX3I CGGCCG 1 cut(s) 178
BseXI GCAGC 4 cut(s) 527, 567, 730, 866
BseYI CCCAGC 1 cut(s) 1385
BsgI GTGCAG 1 cut(s) 720
Bsh1236I CGCG 2 cut(s) 96, 183
Bsh1285I CGRYCG 1 cut(s) 181
BshFI GGCC 8 cut(s) 5, 39, 117, 180, 476, 683, 1102, 1397
BshVI ATCGAT 1 cut(s) 1593
BsiEI CGRYCG 1 cut(s) 181
BsiHKAI GWGCWC 1 cut(s) 712
BsiHKCI CYCGRG 1 cut(s) 1328
BsiSI CCGG 4 cut(s) 6, 33, 177, 570
BslFI GGGAC 1 cut(s) 739
BslI CCNNNNNNNGG 6 cut(s) 214, 265, 307, 576, 615, 1404
BsmAI GTCTC 1 cut(s) 1265
BsmFI GGGAC 1 cut(s) 739
BsnI GGCC 8 cut(s) 5, 39, 117, 180, 476, 683, 1102, 1397
BsoBI CYCGRG 1 cut(s) 1328
Bsp119I TTCGAA 1 cut(s) 1651
Bsp1286I GDGCHC 1 cut(s) 712
Bsp143I GATC 4 cut(s) 127, 217, 971, 997
Bsp19I CCATGG 1 cut(s) 1599
BspACI CCGC 7 cut(s) 51, 79, 94, 181, 208, 266, 606
BspANI GGCC 8 cut(s) 5, 39, 117, 180, 476, 683, 1102, 1397
BspCNI CTCAG 1 cut(s) 238
BspDI ATCGAT 1 cut(s) 1593
BspFNI CGCG 2 cut(s) 96, 183
BspLI GGNNCC 5 cut(s) 270, 568, 777, 927, 1507
BspMAI CTGCAG 2 cut(s) 748, 856
BspMI ACCTGC 1 cut(s) 545
BspPI GGATC 2 cut(s) 225, 1005
BspT104I TTCGAA 1 cut(s) 1651
BspTI CTTAAG 2 cut(s) 1040, 1466
BsrBI CCGCTC 1 cut(s) 53
BsrDI GCAATG 1 cut(s) 1218
BsrFI RCCGGY 1 cut(s) 5
BsrI ACTGG 3 cut(s) 187, 1457, 1491
BssAI RCCGGY 1 cut(s) 5
BssECI CCNNGG 6 cut(s) 40, 112, 609, 836, 1501, 1599
BssMI GATC 4 cut(s) 127, 217, 971, 997
BssNI GRCGYC 1 cut(s) 938
BssSI CACGAG 1 cut(s) 1620
BssT1I CCWWGG 3 cut(s) 836, 1501, 1599
Bst2BI CACGAG 1 cut(s) 1620
Bst2UI CCWGG 1 cut(s) 610
Bst4CI ACNGT 7 cut(s) 124, 518, 730, 933, 1155, 1237, 1246
Bst6I CTCTTC 3 cut(s) 359, 489, 1359
BstACI GRCGYC 1 cut(s) 938
BstAFI CTTAAG 2 cut(s) 1040, 1466
BstBAI YACGTR 1 cut(s) 666
BstBI TTCGAA 1 cut(s) 1651
BstC8I GCNNGC 2 cut(s) 7, 55
BstDEI CTNAG 4 cut(s) 246, 327, 453, 1493
BstDSI CCRYGG 2 cut(s) 40, 1599
BstF5I GGATG 5 cut(s) 75, 436, 924, 1631, 1664
BstFNI CGCG 2 cut(s) 96, 183
BstHHI GCGC 2 cut(s) 185, 487
BstKTI GATC 4 cut(s) 130, 220, 974, 1000
BstMAI GTCTC 1 cut(s) 1265
BstMBI GATC 4 cut(s) 127, 217, 971, 997
BstMCI CGRYCG 1 cut(s) 181
BstMWI GCNNNNNNNGC 9 cut(s) 482, 546, 555, 611, 707, 740, 752, 1229, 1556
BstNI CCWGG 1 cut(s) 610
BstNSI RCATGY 2 cut(s) 237, 881
BstSCI CCNGG 2 cut(s) 568, 608
BstSFI CTRYAG 3 cut(s) 744, 852, 1233
BstUI CGCG 2 cut(s) 96, 183
BstV1I GCAGC 4 cut(s) 527, 567, 730, 866
BstV2I GAAGAC 1 cut(s) 82
BstX2I RGATCY 1 cut(s) 971
BstYI RGATCY 1 cut(s) 971
BstZI CGGCCG 1 cut(s) 178
Bsu15I ATCGAT 1 cut(s) 1593
BsuRI GGCC 8 cut(s) 5, 39, 117, 180, 476, 683, 1102, 1397
BsuTUI ATCGAT 1 cut(s) 1593
BtgI CCRYGG 2 cut(s) 40, 1599
BtgZI GCGATG 1 cut(s) 24
BtsCI GGATG 5 cut(s) 75, 436, 924, 1631, 1664
BtsI GCAGTG 1 cut(s) 1545
BtsIMutI CAGTG 1 cut(s) 1545
BveI ACCTGC 1 cut(s) 545
Cac8I GCNNGC 2 cut(s) 7, 55
CfoI GCGC 2 cut(s) 185, 487
Cfr10I RCCGGY 1 cut(s) 5
Cfr13I GGNCC 6 cut(s) 116, 566, 682, 908, 1100, 1603
ClaI ATCGAT 1 cut(s) 1593
CseI GACGC 4 cut(s) 13, 85, 705, 946
Csp6I GTAC 3 cut(s) 190, 1570, 1662
CviAII CATG 9 cut(s) 70, 234, 262, 413, 686, 878, 1264, 1404, 1600
CviQI GTAC 3 cut(s) 190, 1570, 1662
DdeI CTNAG 4 cut(s) 246, 327, 453, 1493
DpnI GATC 4 cut(s) 129, 219, 973, 999
DpnII GATC 4 cut(s) 127, 217, 971, 997
DraI TTTAAA 2 cut(s) 979, 1428
DrdI GACNNNNNNGTC 1 cut(s) 1054
DseDI GACNNNNNNGTC 1 cut(s) 1054
EaeI YGGCCR 3 cut(s) 3, 178, 474
EagI CGGCCG 1 cut(s) 178
Eam1104I CTCTTC 3 cut(s) 359, 489, 1359
EarI CTCTTC 3 cut(s) 359, 489, 1359
EciI GGCGGA 2 cut(s) 68, 197
Ecl136II GAGCTC 1 cut(s) 710
EclXI CGGCCG 1 cut(s) 178
Eco130I CCWWGG 3 cut(s) 836, 1501, 1599
Eco24I GRGCYC 1 cut(s) 712
Eco47I GGWCC 3 cut(s) 566, 908, 1603
Eco52I CGGCCG 1 cut(s) 178
Eco53kI GAGCTC 1 cut(s) 710
Eco57I CTGAAG 4 cut(s) 741, 1013, 1322, 1502
Eco88I CYCGRG 1 cut(s) 1328
EcoICRI GAGCTC 1 cut(s) 710
EcoRII CCWGG 1 cut(s) 608
EcoT14I CCWWGG 3 cut(s) 836, 1501, 1599
EcoT22I ATGCAT 1 cut(s) 1150
EcoT38I GRGCYC 1 cut(s) 712
ErhI CCWWGG 3 cut(s) 836, 1501, 1599
FaeI CATG 9 cut(s) 73, 237, 265, 416, 689, 881, 1267, 1407, 1603
FaqI GGGAC 1 cut(s) 739
FatI CATG 9 cut(s) 69, 233, 261, 412, 685, 877, 1263, 1403, 1599
FauNDI CATATG 1 cut(s) 913
Fnu4HI GCNGC 8 cut(s) 51, 181, 267, 541, 556, 606, 744, 855
FokI GGATG 5 cut(s) 62, 443, 931, 1638, 1651
FriOI GRGCYC 1 cut(s) 712
Fsp4HI GCNGC 8 cut(s) 51, 181, 267, 541, 556, 606, 744, 855
FspBI CTAG 7 cut(s) 381, 390, 552, 713, 812, 1556, 1607
GlaI GCGC 2 cut(s) 184, 486
GluI GCNGC 8 cut(s) 51, 181, 267, 541, 556, 606, 744, 855
GsaI CCCAGC 1 cut(s) 1389
HaeIII GGCC 8 cut(s) 5, 39, 117, 180, 476, 683, 1102, 1397
HapII CCGG 4 cut(s) 6, 33, 177, 570
HgaI GACGC 4 cut(s) 13, 85, 705, 946
HhaI GCGC 2 cut(s) 185, 487
Hin1I GRCGYC 1 cut(s) 938
Hin1II CATG 9 cut(s) 73, 237, 265, 416, 689, 881, 1267, 1407, 1603
Hin6I GCGC 2 cut(s) 183, 485
HinP1I GCGC 2 cut(s) 183, 485
HincII GTYRAC 1 cut(s) 936
HindII GTYRAC 1 cut(s) 936
HindIII AAGCTT 4 cut(s) 899, 1110, 1334, 1611
HinfI GANTC 6 cut(s) 102, 143, 153, 434, 1055, 1258
HpaII CCGG 4 cut(s) 6, 33, 177, 570
HphI GGTGA 2 cut(s) 22, 584
Hpy166II GTNNAC 4 cut(s) 891, 936, 1269, 1570
Hpy188I TCNGA 6 cut(s) 101, 142, 566, 638, 721, 1363
Hpy188III TCNNGA 5 cut(s) 131, 812, 945, 1031, 1330
Hpy8I GTNNAC 4 cut(s) 891, 936, 1269, 1570
Hpy99I CGWCG 2 cut(s) 29, 128
HpyAV CCTTC 9 cut(s) 147, 314, 716, 809, 916, 951, 1091, 1153, 1570
HpyCH4III ACNGT 7 cut(s) 124, 518, 730, 933, 1155, 1237, 1246
HpyCH4IV ACGT 3 cut(s) 87, 665, 678
HpyF10VI GCNNNNNNNGC 9 cut(s) 482, 546, 555, 611, 707, 740, 752, 1229, 1556
HpyF3I CTNAG 4 cut(s) 246, 327, 453, 1493
HpySE526I ACGT 3 cut(s) 87, 665, 678
Hsp92I GRCGYC 1 cut(s) 938
Hsp92II CATG 9 cut(s) 73, 237, 265, 416, 689, 881, 1267, 1407, 1603
HspAI GCGC 2 cut(s) 183, 485
KroI GCCGGC 1 cut(s) 5
KroNI GCCGGC 1 cut(s) 7
Kzo9I GATC 4 cut(s) 127, 217, 971, 997
LmnI GCTCC 3 cut(s) 274, 303, 1505
Lsp1109I GCAGC 4 cut(s) 527, 567, 730, 866
LweI GCATC 7 cut(s) 154, 331, 855, 1135, 1232, 1585, 1616
MaeI CTAG 7 cut(s) 381, 390, 552, 713, 812, 1556, 1607
MaeII ACGT 3 cut(s) 87, 665, 678
MaeIII GTNAC 5 cut(s) 28, 579, 801, 1007, 1371
MalI GATC 4 cut(s) 129, 219, 973, 999
MbiI CCGCTC 1 cut(s) 53
MboI GATC 4 cut(s) 127, 217, 971, 997
MboII GAAGA 7 cut(s) 82, 218, 376, 506, 975, 1376, 1595
MfeI CAATTG 1 cut(s) 870
MflI RGATCY 1 cut(s) 971
MhlI GDGCHC 1 cut(s) 712
MlyI GAGTC 5 cut(s) 152, 162, 443, 1064, 1267
MmeI TCCRAC 1 cut(s) 171
Mph1103I ATGCAT 1 cut(s) 1150
MroNI GCCGGC 1 cut(s) 5
MseI TTAA 8 cut(s) 522, 596, 897, 978, 1041, 1353, 1427, 1467
MslI CAYNNNNRTG 2 cut(s) 696, 1153
MspA1I CMGCKG 1 cut(s) 857
MspCI CTTAAG 2 cut(s) 1040, 1466
MspI CCGG 4 cut(s) 6, 33, 177, 570
MspR9I CCNGG 2 cut(s) 570, 610
MunI CAATTG 1 cut(s) 870
MvaI CCWGG 1 cut(s) 610
MvnI CGCG 2 cut(s) 96, 183
MwoI GCNNNNNNNGC 9 cut(s) 482, 546, 555, 611, 707, 740, 752, 1229, 1556
NaeI GCCGGC 1 cut(s) 7
NciI CCSGG 1 cut(s) 570
NcoI CCATGG 1 cut(s) 1599
NdeI CATATG 1 cut(s) 913
NdeII GATC 4 cut(s) 127, 217, 971, 997
NgoMIV GCCGGC 1 cut(s) 5
NlaIII CATG 9 cut(s) 73, 237, 265, 416, 689, 881, 1267, 1407, 1603
NlaIV GGNNCC 5 cut(s) 270, 568, 777, 927, 1507
NmeAIII GCCGAG 1 cut(s) 93
NmuCI GTSAC 3 cut(s) 28, 801, 1371
NsiI ATGCAT 1 cut(s) 1150
NspI RCATGY 2 cut(s) 237, 881
NspV TTCGAA 1 cut(s) 1651
PaeR7I CTCGAG 1 cut(s) 1328
PciI ACATGT 1 cut(s) 877
PcsI WCGNNNNNNNCGW 3 cut(s) 93, 120, 138
PdiI GCCGGC 1 cut(s) 7
PfeI GAWTC 1 cut(s) 102
PkrI GCNGC 8 cut(s) 52, 182, 268, 542, 557, 607, 745, 856
PleI GAGTC 5 cut(s) 151, 161, 442, 1063, 1266
PpsI GAGTC 5 cut(s) 151, 161, 442, 1063, 1266
Ppu21I YACGTR 1 cut(s) 666
PscI ACATGT 1 cut(s) 877
PsiI TTATAA 1 cut(s) 764
Psp124BI GAGCTC 1 cut(s) 712
Psp6I CCWGG 1 cut(s) 608
PspFI CCCAGC 1 cut(s) 1385
PspGI CCWGG 1 cut(s) 608
PspN4I GGNNCC 5 cut(s) 270, 568, 777, 927, 1507
PspPI GGNCC 6 cut(s) 116, 566, 682, 908, 1100, 1603
PstI CTGCAG 2 cut(s) 748, 856
PsuI RGATCY 1 cut(s) 971
PvuII CAGCTG 1 cut(s) 857
RsaI GTAC 3 cut(s) 191, 1571, 1663
RsaNI GTAC 3 cut(s) 190, 1570, 1662
RseI CAYNNNNRTG 2 cut(s) 696, 1153
SacI GAGCTC 1 cut(s) 712
SaqAI TTAA 8 cut(s) 522, 596, 897, 978, 1041, 1353, 1427, 1467
SatI GCNGC 8 cut(s) 51, 181, 267, 541, 556, 606, 744, 855
Sau3AI GATC 4 cut(s) 127, 217, 971, 997
Sau96I GGNCC 6 cut(s) 116, 566, 682, 908, 1100, 1603
SchI GAGTC 5 cut(s) 152, 162, 443, 1064, 1267
ScrFI CCNGG 2 cut(s) 570, 610
SduI GDGCHC 1 cut(s) 712
SfaNI GCATC 7 cut(s) 154, 331, 855, 1135, 1232, 1585, 1616
SfcI CTRYAG 3 cut(s) 744, 852, 1233
Sfr274I CTCGAG 1 cut(s) 1328
SfuI TTCGAA 1 cut(s) 1651
SinI GGWCC 3 cut(s) 566, 908, 1603
SlaI CTCGAG 1 cut(s) 1328
SmiMI CAYNNNNRTG 2 cut(s) 696, 1153
SmlI CTYRAG 5 cut(s) 1040, 1069, 1227, 1328, 1466
SmoI CTYRAG 5 cut(s) 1040, 1069, 1227, 1328, 1466
SsiI CCGC 7 cut(s) 51, 79, 94, 181, 208, 266, 606
SspMI CTAG 7 cut(s) 381, 390, 552, 713, 812, 1556, 1607
SstI GAGCTC 1 cut(s) 712
StyD4I CCNGG 2 cut(s) 568, 608
StyI CCWWGG 3 cut(s) 836, 1501, 1599
TaaI ACNGT 7 cut(s) 124, 518, 730, 933, 1155, 1237, 1246
TaiI ACGT 3 cut(s) 90, 668, 681
TaqI TCGA 9 cut(s) 126, 132, 201, 1000, 1053, 1084, 1329, 1593, 1651
TaqII GACCGA 1 cut(s) 298
TatI WGTACW 2 cut(s) 189, 1661
TauI GCSGC 4 cut(s) 53, 183, 269, 608
TfiI GAWTC 1 cut(s) 102
Tru1I TTAA 8 cut(s) 522, 596, 897, 978, 1041, 1353, 1427, 1467
Tru9I TTAA 8 cut(s) 522, 596, 897, 978, 1041, 1353, 1427, 1467
TscAI CASTG 1 cut(s) 1552
TseFI GTSAC 3 cut(s) 28, 801, 1371
TseI GCWGC 4 cut(s) 540, 555, 743, 854
Tsp45I GTSAC 3 cut(s) 28, 801, 1371
TspDTI ATGAA 6 cut(s) 401, 619, 843, 935, 1207, 1276
TspGWI ACGGA 1 cut(s) 165
TspRI CASTG 1 cut(s) 1552
Vha464I CTTAAG 2 cut(s) 1040, 1466
VpaK11BI GGWCC 3 cut(s) 566, 908, 1603
XapI RAATTY 1 cut(s) 1655
XbaI TCTAGA 1 cut(s) 811
XceI RCATGY 2 cut(s) 237, 881
XhoI CTCGAG 1 cut(s) 1328
XspI CTAG 7 cut(s) 381, 390, 552, 713, 812, 1556, 1607
Zsp2I ATGCAT 1 cut(s) 1150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.