Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0003789

Physical Location & Seq

Genomic Coordinates Forward (+)

62951723 .. 62956207

Gene Structure 4485 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G038850.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1836 bp

ATGGTCCGTCGTCAACCCGACTTCTGTGATTTTCTTCTTCTTAGCTTTTGTCACCTTCGTCTCTTCAGTCCAACTCACTCTCTCTCTCTCTCTCTCTCAGTGCCTCAGATCTCTAATTCACCTGAAAGCTTCGTTATCTGTTTCACTTCACCGATGGCCGGCGATGAGAGATGGTGCGTCGTCACCGGAGGCCGAGGCTTCGCCGCTCGCCATTTGGTTCTCATGCTCATCCGCCAAAACGTCTTCCGCGTCCGAATCGCCGACCTCGGCCCCACCGTCGATCTCGAACCTTCCGAGTCCAACGGAGTCCTCGCCGATGCTTTGAACTCCGGCCGCGCCCAGTACATTTCCATCGACCTCCGCCGTAAGGATCAAGTTCTTCAAGCATGTCAAGGTGCTGAGGCTGTGTTCCACATGGCGGCTCCCAATTCGTCGATCAACAATTACCAGCTCCACCATTCGGTCAATGTGGAAGGAACTAAGAATGTGATTGATGCTTGTGTTGAGCAGAAAGTGAAGAGGCTCATATACACTAGCTCTCCTAGTGTTGTGTTTGATGGGGTCCATGGCATTATTGATGGGGATGAGTCATTGCCATATCCACCTAAGCACAATGATACTTATTCGGCCACTAAAGCGCAAGGAGAAGAGTTGGTAATCAAGGCAAACGGTGTTAAAGGGCTTCTAACCTGCTGCATACGCCCTAGCAGCATTTTCGGACCCGGTGATAGGTTACTTGTTCCATCTTTAATTGTTGCCGCCAGGGCAGGGAAATCCAAGTTCATTATCGGAGATGGCAATAACATTTATGACTTCACGTATGTTGAAAACGTGGCCCATTCCCATATATGTGCAGAACGAGCTCTAGCGTCTGAAGGGACAGTTGCAGAGAAAGCTGCAGGACAGGCATATTTTATAACCAATATGGAACCTATCAAGTTTTGGGAGTTTATGTCACTTATTCTAGAAGGTCTTGGCTATGAAAGACCAAGAATAAAAATCCCTGCAGCTGTTATGATGCCAATTGCACATGTGGTGGAGTGGACATTTAAGCTTTTTGGTCCATATGGGATGAAGGTTCCACAGTTGACGCCTTCAAGAATTAGACTTCTCTCTTGCACCAGATCTTTTAAATCTTCAAAAGCACAAGATCGAATTGGTTACACGCCCATCATTTCACTTCAGGAGGGTCTTAAGAGGACAATCGAGTCATACCCACACTTGAGGGCTGAACATCGACCCAAAAGAGAAGGGCCATCTAAAGCTTCTGTGTATCTTGGAAGTGGGAGGGTTGCTGATGCATTACTGTGGAAGGACAAAAAACAGACATTCAAGGCACTATTATTTTTTATTGCAATCTACTACAACTTCATTGCATCTCAAGCTACAGTTATTACCGTCATTTCAAAGAGTCTCATGTTCACATCAATTTTCGTGTTCATCTCTGGCAATTTACCAGAAAAGATATTAGGATATAAACTCGAGAAGCTTCAGTCATCACATTTTAACTTATCAGAAGAGATGTCACAACAAATTGCTGGGTCGGTGGCCTCATCATGGAATATATCAGTGAAAACTTTAAAGTCTCTTGGGAAAGGAAATGACTGGATATTGTTTCTTAAGGTGGTTCTATCCTTACTGGTTCTTAGCTTCCTTGGAGCCATTTCACTTCAGCACTCATTTATTATAGGAGTTTCCACTGCCTTTCTAGCCTTCTATGTGTACGAAAAGCAGGAAGAAAAAATCGATGCCATGGTCCTAGAAGCTTTCTCTCGTGGATGCAAATTGAAGTCCAATATGCTCCGAAAATTCTGTACATCCAAAAAGATTGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0008150 GO:0008152 GO:0016020 GO:0016491 GO:0016614 GO:0016616 GO:0047012 GO:0055114

KEGG Pathways

Metabolic & Signaling

ko00100 ko01100 ko01130 map00100 map01100 map01130

Pfam Domains

Protein Families

3Beta_HSD Reticulon

Protein Analysis

611

Amino Acids

67.83

Weight (kDa)

9.19

Isoelectric Point (pI)

42.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
3Beta_HSD	PF01073	60 - 334	5.7e-77	3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase	PF01370	60 - 288	3.5e-33	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	60 - 180	3.8e-07	Polysaccharide biosynthesis protein
GDP_Man_Dehyd	PF16363	61 - 283	4e-14	GDP-mannose 4,6 dehydratase
NAD_binding_10	PF13460	108 - 184	1.6e-08	NAD(P)H-binding
RmlD_sub_bind	PF04321	118 - 333	2.2e-07	RmlD substrate binding domain
NAD_binding_4	PF07993	127 - 278	5.9e-13	Male sterility protein
Reticulon	PF02453	431 - 586	3.6e-31	Reticulon

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003789)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47290 AT1G47290 AT2G26260 AT2G26260
fragaria_vesca	FvH4_2g36970 FvH4_2g36970
malus_domestica	MD08G1046500.v1.1
prunus_persica	Prupe.1G401700_v2.0.a1 Prupe.1G401700_v2.0.a1
pyrus_communis	pycom08g03860 pycom15g04580 pycom15g04640
rosa_chinensis	RchiOBHm_Chr4g0406951 RchiOBHm_Chr6g0304791
rosa_laevigata	RLG00000008747 RLG00000010949
rosa_multiflora	Rmu_sc0000600.1_g000025 Rmu_sc0003120.1_g000003
rosa_roxburghii	Rroxscaffold_7G00163470
rosa_rugosa	Rorug03G0259800 Rorug04G0075400 Rorug06G0334600 Rorug06G0334600 Rorug06G0334700 Rorug06G0334800
rosa_samantha	Rh4AG141600 Rh4BG138400 Rh4DG135500 Rh6AG446500 Rh6BG455100 Rh6CG460000 Rh6DG446800
rosa_wichuraiana	Rw4G011530 Rw6G038850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	917
AasI	GACNNNNNNGTC	1 cut(s)	1207
Acc36I	ACCTGC	1 cut(s)	698
AccBSI	CCGCTC	1 cut(s)	206
AccII	CGCG	2 cut(s)	249, 336
AciI	CCGC	7 cut(s)	204, 232, 247, 334, 361, 419, 759
AclWI	GGATC	1 cut(s)	378
AcoI	YGGCCR	3 cut(s)	156, 331, 627
AcsI	RAATTY	1 cut(s)	1808
AcuI	CTGAAG	5 cut(s)	49, 894, 1166, 1475, 1655
AcyI	GRCGYC	1 cut(s)	1091
AfaI	GTAC	3 cut(s)	344, 1724, 1816
AfiI	CCNNNNNNNGG	7 cut(s)	158, 367, 418, 460, 729, 768, 1557
AflII	CTTAAG	2 cut(s)	1193, 1619
AflIII	ACRYGT	1 cut(s)	1030
AgsI	TTSAA	8 cut(s)	325, 383, 827, 1098, 1140, 1333, 1407, 1789
AjnI	CCWGG	1 cut(s)	761
Alw21I	GWGCWC	1 cut(s)	865
Alw26I	GTCTC	3 cut(s)	65, 1418, 1590
AlwI	GGATC	1 cut(s)	378
Ama87I	CYCGRG	1 cut(s)	1481
AoxI	GGCC	8 cut(s)	156, 190, 268, 331, 627, 834, 1253, 1548
ApeKI	GCWGC	4 cut(s)	693, 708, 896, 1007
ApoI	RAATTY	1 cut(s)	1808
AspLEI	GCGC	2 cut(s)	338, 640
AspS9I	GGNCC	8 cut(s)	4, 269, 562, 719, 835, 1061, 1253, 1756
AsuC2I	CCSGG	1 cut(s)	723
AsuHPI	GGTGA	5 cut(s)	44, 111, 141, 175, 737
AvaI	CYCGRG	1 cut(s)	1481
AvaII	GGWCC	5 cut(s)	4, 562, 719, 1061, 1756
BanII	GRGCYC	1 cut(s)	865
BauI	CACGAG	1 cut(s)	1773
BbsI	GAAGAC	1 cut(s)	235
Bbv12I	GWGCWC	1 cut(s)	865
BbvCI	CCTCAGC	1 cut(s)	399
BbvI	GCAGC	4 cut(s)	680, 720, 883, 1019
BccI	CCATC	9 cut(s)	148, 165, 359, 551, 572, 751, 788, 1178, 1264
BceAI	ACGGC	1 cut(s)	348
BcgI	CGANNNNNNTGC	2 cut(s)	697, 731
BciT130I	CCWGG	1 cut(s)	763
BcnI	CCSGG	1 cut(s)	723
BcoDI	GTCTC	3 cut(s)	65, 1418, 1590
BfaI	CTAG	7 cut(s)	534, 543, 705, 866, 965, 1709, 1760
BfmI	CTRYAG	3 cut(s)	897, 1005, 1386
BfrI	CTTAAG	2 cut(s)	1193, 1619
BfuAI	ACCTGC	1 cut(s)	698
BglI	GCCNNNNNGGC	1 cut(s)	764
BglII	AGATCT	2 cut(s)	108, 1124
BisI	GCNGC	8 cut(s)	204, 334, 420, 694, 709, 759, 897, 1008
BlsI	GCNGC	8 cut(s)	205, 335, 421, 695, 710, 760, 898, 1009
Bme1390I	CCNGG	2 cut(s)	723, 763
Bme18I	GGWCC	5 cut(s)	4, 562, 719, 1061, 1756
BmeT110I	CYCGRG	1 cut(s)	1481
BmgT120I	GGNCC	8 cut(s)	4, 269, 562, 719, 835, 1061, 1253, 1756
BmiI	GGNNCC	7 cut(s)	271, 423, 563, 721, 930, 1080, 1660
BmrFI	CCNGG	2 cut(s)	723, 763
BmrI	ACTGGG	1 cut(s)	334
BmsI	GCATC	7 cut(s)	307, 484, 1008, 1288, 1385, 1738, 1769
BmuI	ACTGGG	1 cut(s)	334
BpiI	GAAGAC	1 cut(s)	235
Bpu10I	CCTNAGC	2 cut(s)	399, 606
BpuEI	CTTGAG	2 cut(s)	1243, 1365
BpuMI	CCSGG	1 cut(s)	723
Bsa29I	ATCGAT	1 cut(s)	1746
BsaAI	YACGTR	1 cut(s)	819
BsaHI	GRCGYC	1 cut(s)	1091
BsaJI	CCNNGG	6 cut(s)	193, 265, 565, 762, 1654, 1752
BsaWI	WCCGGW	1 cut(s)	185
BsaXI	ACNNNNNCTCC	4 cut(s)	523, 553, 938, 968
Bsc4I	CCNNNNNNNGG	7 cut(s)	158, 367, 418, 460, 729, 768, 1557
Bse118I	RCCGGY	1 cut(s)	158
Bse1I	ACTGG	3 cut(s)	340, 1610, 1644
Bse3DI	GCAATG	2 cut(s)	590, 1371
BseBI	CCWGG	1 cut(s)	763
BseCI	ATCGAT	1 cut(s)	1746
BseDI	CCNNGG	6 cut(s)	193, 265, 565, 762, 1654, 1752
BseGI	GGATG	5 cut(s)	228, 589, 1077, 1784, 1817
BseLI	CCNNNNNNNGG	7 cut(s)	158, 367, 418, 460, 729, 768, 1557
BseMI	GCAATG	2 cut(s)	590, 1371
BseMII	CTCAG	3 cut(s)	111, 119, 390
BseNI	ACTGG	3 cut(s)	340, 1610, 1644
BseX3I	CGGCCG	1 cut(s)	331
BseXI	GCAGC	4 cut(s)	680, 720, 883, 1019
BseYI	CCCAGC	1 cut(s)	1538
BsgI	GTGCAG	1 cut(s)	873
Bsh1236I	CGCG	2 cut(s)	249, 336
Bsh1285I	CGRYCG	1 cut(s)	334
BshFI	GGCC	8 cut(s)	158, 192, 270, 333, 629, 836, 1255, 1550
BshVI	ATCGAT	1 cut(s)	1746
BsiEI	CGRYCG	1 cut(s)	334
BsiHKAI	GWGCWC	1 cut(s)	865
BsiHKCI	CYCGRG	1 cut(s)	1481
BsiSI	CCGG	4 cut(s)	159, 186, 330, 723
BslFI	GGGAC	1 cut(s)	892
BslI	CCNNNNNNNGG	7 cut(s)	158, 367, 418, 460, 729, 768, 1557
BsmAI	GTCTC	3 cut(s)	65, 1418, 1590
BsmBI	CGTCTC	1 cut(s)	65
BsmFI	GGGAC	1 cut(s)	892
BsnI	GGCC	8 cut(s)	158, 192, 270, 333, 629, 836, 1255, 1550
BsoBI	CYCGRG	1 cut(s)	1481
Bsp1286I	GDGCHC	1 cut(s)	865
Bsp1407I	TGTACA	1 cut(s)	1814
Bsp143I	GATC	6 cut(s)	108, 280, 370, 435, 1124, 1150
Bsp19I	CCATGG	2 cut(s)	565, 1752
BspACI	CCGC	7 cut(s)	204, 232, 247, 334, 361, 419, 759
BspANI	GGCC	8 cut(s)	158, 192, 270, 333, 629, 836, 1255, 1550
BspCNI	CTCAG	3 cut(s)	110, 118, 391
BspDI	ATCGAT	1 cut(s)	1746
BspFNI	CGCG	2 cut(s)	249, 336
BspLI	GGNNCC	7 cut(s)	271, 423, 563, 721, 930, 1080, 1660
BspMAI	CTGCAG	2 cut(s)	901, 1009
BspMI	ACCTGC	1 cut(s)	698
BspPI	GGATC	1 cut(s)	378
BspTI	CTTAAG	2 cut(s)	1193, 1619
BsrBI	CCGCTC	1 cut(s)	206
BsrDI	GCAATG	2 cut(s)	590, 1371
BsrFI	RCCGGY	1 cut(s)	158
BsrGI	TGTACA	1 cut(s)	1814
BsrI	ACTGG	3 cut(s)	340, 1610, 1644
BssAI	RCCGGY	1 cut(s)	158
BssECI	CCNNGG	6 cut(s)	193, 265, 565, 762, 1654, 1752
BssMI	GATC	6 cut(s)	108, 280, 370, 435, 1124, 1150
BssNI	GRCGYC	1 cut(s)	1091
BssSI	CACGAG	1 cut(s)	1773
BssT1I	CCWWGG	3 cut(s)	565, 1654, 1752
Bst2BI	CACGAG	1 cut(s)	1773
Bst2UI	CCWGG	1 cut(s)	763
Bst4CI	ACNGT	7 cut(s)	277, 671, 883, 1086, 1308, 1390, 1399
Bst6I	CTCTTC	4 cut(s)	68, 512, 642, 1512
BstACI	GRCGYC	1 cut(s)	1091
BstAFI	CTTAAG	2 cut(s)	1193, 1619
BstAUI	TGTACA	1 cut(s)	1814
BstBAI	YACGTR	1 cut(s)	819
BstC8I	GCNNGC	2 cut(s)	160, 208
BstDEI	CTNAG	7 cut(s)	41, 97, 105, 399, 480, 606, 1646
BstDSI	CCRYGG	2 cut(s)	565, 1752
BstF5I	GGATG	5 cut(s)	228, 589, 1077, 1784, 1817
BstFNI	CGCG	2 cut(s)	249, 336
BstHHI	GCGC	2 cut(s)	338, 640
BstKTI	GATC	6 cut(s)	111, 283, 373, 438, 1127, 1153
BstMAI	GTCTC	3 cut(s)	65, 1418, 1590
BstMBI	GATC	6 cut(s)	108, 280, 370, 435, 1124, 1150
BstMCI	CGRYCG	1 cut(s)	334
BstMWI	GCNNNNNNNGC	9 cut(s)	635, 699, 708, 764, 860, 893, 905, 1382, 1709
BstNI	CCWGG	1 cut(s)	763
BstNSI	RCATGY	2 cut(s)	390, 1034
BstSCI	CCNGG	2 cut(s)	721, 761
BstSFI	CTRYAG	3 cut(s)	897, 1005, 1386
BstUI	CGCG	2 cut(s)	249, 336
BstV1I	GCAGC	4 cut(s)	680, 720, 883, 1019
BstV2I	GAAGAC	1 cut(s)	235
BstX2I	RGATCY	2 cut(s)	108, 1124
BstYI	RGATCY	2 cut(s)	108, 1124
BstZI	CGGCCG	1 cut(s)	331
Bsu15I	ATCGAT	1 cut(s)	1746
BsuRI	GGCC	8 cut(s)	158, 192, 270, 333, 629, 836, 1255, 1550
BsuTUI	ATCGAT	1 cut(s)	1746
BtgI	CCRYGG	2 cut(s)	565, 1752
BtgZI	GCGATG	1 cut(s)	177
BtsCI	GGATG	5 cut(s)	228, 589, 1077, 1784, 1817
BtsI	GCAGTG	1 cut(s)	1698
BtsIMutI	CAGTG	3 cut(s)	105, 1575, 1698
BveI	ACCTGC	1 cut(s)	698
Cac8I	GCNNGC	2 cut(s)	160, 208
CfoI	GCGC	2 cut(s)	338, 640
Cfr10I	RCCGGY	1 cut(s)	158
Cfr13I	GGNCC	8 cut(s)	4, 269, 562, 719, 835, 1061, 1253, 1756
ClaI	ATCGAT	1 cut(s)	1746
CseI	GACGC	4 cut(s)	166, 238, 858, 1099
Csp6I	GTAC	3 cut(s)	343, 1723, 1815
CviAII	CATG	8 cut(s)	223, 387, 415, 566, 1031, 1417, 1557, 1753
CviQI	GTAC	3 cut(s)	343, 1723, 1815
DdeI	CTNAG	7 cut(s)	41, 97, 105, 399, 480, 606, 1646
DpnI	GATC	6 cut(s)	110, 282, 372, 437, 1126, 1152
DpnII	GATC	6 cut(s)	108, 280, 370, 435, 1124, 1150
DraI	TTTAAA	2 cut(s)	1132, 1581
DrdI	GACNNNNNNGTC	1 cut(s)	1207
DseDI	GACNNNNNNGTC	1 cut(s)	1207
EaeI	YGGCCR	3 cut(s)	156, 331, 627
EagI	CGGCCG	1 cut(s)	331
Eam1104I	CTCTTC	4 cut(s)	68, 512, 642, 1512
EarI	CTCTTC	4 cut(s)	68, 512, 642, 1512
EciI	GGCGGA	2 cut(s)	221, 350
Ecl136II	GAGCTC	1 cut(s)	863
EclXI	CGGCCG	1 cut(s)	331
Eco130I	CCWWGG	3 cut(s)	565, 1654, 1752
Eco24I	GRGCYC	1 cut(s)	865
Eco47I	GGWCC	5 cut(s)	4, 562, 719, 1061, 1756
Eco52I	CGGCCG	1 cut(s)	331
Eco53kI	GAGCTC	1 cut(s)	863
Eco57I	CTGAAG	5 cut(s)	49, 894, 1166, 1475, 1655
Eco88I	CYCGRG	1 cut(s)	1481
EcoICRI	GAGCTC	1 cut(s)	863
EcoRII	CCWGG	1 cut(s)	761
EcoT14I	CCWWGG	3 cut(s)	565, 1654, 1752
EcoT22I	ATGCAT	1 cut(s)	1303
EcoT38I	GRGCYC	1 cut(s)	865
ErhI	CCWWGG	3 cut(s)	565, 1654, 1752
Esp3I	CGTCTC	1 cut(s)	65
FaeI	CATG	8 cut(s)	226, 390, 418, 569, 1034, 1420, 1560, 1756
FaqI	GGGAC	1 cut(s)	892
FatI	CATG	8 cut(s)	222, 386, 414, 565, 1030, 1416, 1556, 1752
FauNDI	CATATG	1 cut(s)	1066
Fnu4HI	GCNGC	8 cut(s)	204, 334, 420, 694, 709, 759, 897, 1008
FokI	GGATG	5 cut(s)	215, 596, 1084, 1791, 1804
FriOI	GRGCYC	1 cut(s)	865
Fsp4HI	GCNGC	8 cut(s)	204, 334, 420, 694, 709, 759, 897, 1008
FspBI	CTAG	7 cut(s)	534, 543, 705, 866, 965, 1709, 1760
GlaI	GCGC	2 cut(s)	337, 639
GluI	GCNGC	8 cut(s)	204, 334, 420, 694, 709, 759, 897, 1008
GsaI	CCCAGC	1 cut(s)	1542
HaeIII	GGCC	8 cut(s)	158, 192, 270, 333, 629, 836, 1255, 1550
HapII	CCGG	4 cut(s)	159, 186, 330, 723
HgaI	GACGC	4 cut(s)	166, 238, 858, 1099
HhaI	GCGC	2 cut(s)	338, 640
Hin1I	GRCGYC	1 cut(s)	1091
Hin1II	CATG	8 cut(s)	226, 390, 418, 569, 1034, 1420, 1560, 1756
Hin6I	GCGC	2 cut(s)	336, 638
HinP1I	GCGC	2 cut(s)	336, 638
HincII	GTYRAC	2 cut(s)	14, 1089
HindII	GTYRAC	2 cut(s)	14, 1089
HindIII	AAGCTT	5 cut(s)	127, 1052, 1263, 1487, 1764
HinfI	GANTC	6 cut(s)	255, 296, 306, 587, 1208, 1411
HpaII	CCGG	4 cut(s)	159, 186, 330, 723
HphI	GGTGA	5 cut(s)	44, 111, 141, 175, 737
Hpy166II	GTNNAC	5 cut(s)	14, 1044, 1089, 1422, 1723
Hpy188I	TCNGA	8 cut(s)	108, 254, 295, 719, 791, 874, 1516, 1805
Hpy188III	TCNNGA	5 cut(s)	284, 965, 1098, 1184, 1483
Hpy8I	GTNNAC	5 cut(s)	14, 1044, 1089, 1422, 1723
Hpy99I	CGWCG	4 cut(s)	12, 182, 281, 436
HpyCH4III	ACNGT	7 cut(s)	277, 671, 883, 1086, 1308, 1390, 1399
HpyCH4IV	ACGT	3 cut(s)	240, 818, 831
HpyF10VI	GCNNNNNNNGC	9 cut(s)	635, 699, 708, 764, 860, 893, 905, 1382, 1709
HpyF3I	CTNAG	7 cut(s)	41, 97, 105, 399, 480, 606, 1646
HpySE526I	ACGT	3 cut(s)	240, 818, 831
Hsp92I	GRCGYC	1 cut(s)	1091
Hsp92II	CATG	8 cut(s)	226, 390, 418, 569, 1034, 1420, 1560, 1756
HspAI	GCGC	2 cut(s)	336, 638
KroI	GCCGGC	1 cut(s)	158
KroNI	GCCGGC	1 cut(s)	160
Kzo9I	GATC	6 cut(s)	108, 280, 370, 435, 1124, 1150
LmnI	GCTCC	4 cut(s)	427, 456, 1658, 1806
Lsp1109I	GCAGC	4 cut(s)	680, 720, 883, 1019
LweI	GCATC	7 cut(s)	307, 484, 1008, 1288, 1385, 1738, 1769
MaeI	CTAG	7 cut(s)	534, 543, 705, 866, 965, 1709, 1760
MaeII	ACGT	3 cut(s)	240, 818, 831
MaeIII	GTNAC	6 cut(s)	50, 181, 732, 954, 1160, 1524
MalI	GATC	6 cut(s)	110, 282, 372, 437, 1126, 1152
MbiI	CCGCTC	1 cut(s)	206
MboI	GATC	6 cut(s)	108, 280, 370, 435, 1124, 1150
MfeI	CAATTG	1 cut(s)	1023
MflI	RGATCY	2 cut(s)	108, 1124
MhlI	GDGCHC	1 cut(s)	865
MlyI	GAGTC	5 cut(s)	305, 315, 596, 1217, 1420
MmeI	TCCRAC	2 cut(s)	95, 324
Mph1103I	ATGCAT	1 cut(s)	1303
MroNI	GCCGGC	1 cut(s)	158
MseI	TTAA	8 cut(s)	675, 749, 1050, 1131, 1194, 1506, 1580, 1620
MslI	CAYNNNNRTG	2 cut(s)	849, 1306
MspA1I	CMGCKG	1 cut(s)	1010
MspCI	CTTAAG	2 cut(s)	1193, 1619
MspI	CCGG	4 cut(s)	159, 186, 330, 723
MspR9I	CCNGG	2 cut(s)	723, 763
MunI	CAATTG	1 cut(s)	1023
MvaI	CCWGG	1 cut(s)	763
MvnI	CGCG	2 cut(s)	249, 336
MwoI	GCNNNNNNNGC	9 cut(s)	635, 699, 708, 764, 860, 893, 905, 1382, 1709
NaeI	GCCGGC	1 cut(s)	160
NciI	CCSGG	1 cut(s)	723
NcoI	CCATGG	2 cut(s)	565, 1752
NdeI	CATATG	1 cut(s)	1066
NdeII	GATC	6 cut(s)	108, 280, 370, 435, 1124, 1150
NgoMIV	GCCGGC	1 cut(s)	158
NlaIII	CATG	8 cut(s)	226, 390, 418, 569, 1034, 1420, 1560, 1756
NlaIV	GGNNCC	7 cut(s)	271, 423, 563, 721, 930, 1080, 1660
NmeAIII	GCCGAG	2 cut(s)	218, 246
NmuCI	GTSAC	4 cut(s)	50, 181, 954, 1524
NsiI	ATGCAT	1 cut(s)	1303
NspI	RCATGY	2 cut(s)	390, 1034
PaeR7I	CTCGAG	1 cut(s)	1481
PciI	ACATGT	1 cut(s)	1030
PcsI	WCGNNNNNNNCGW	3 cut(s)	246, 273, 291
PdiI	GCCGGC	1 cut(s)	160
PfeI	GAWTC	1 cut(s)	255
PkrI	GCNGC	8 cut(s)	205, 335, 421, 695, 710, 760, 898, 1009
PleI	GAGTC	5 cut(s)	304, 314, 595, 1216, 1419
PpsI	GAGTC	5 cut(s)	304, 314, 595, 1216, 1419
Ppu21I	YACGTR	1 cut(s)	819
PscI	ACATGT	1 cut(s)	1030
PsiI	TTATAA	1 cut(s)	917
Psp124BI	GAGCTC	1 cut(s)	865
Psp6I	CCWGG	1 cut(s)	761
PspFI	CCCAGC	1 cut(s)	1538
PspGI	CCWGG	1 cut(s)	761
PspN4I	GGNNCC	7 cut(s)	271, 423, 563, 721, 930, 1080, 1660
PspPI	GGNCC	8 cut(s)	4, 269, 562, 719, 835, 1061, 1253, 1756
PstI	CTGCAG	2 cut(s)	901, 1009
PsuI	RGATCY	2 cut(s)	108, 1124
PvuII	CAGCTG	1 cut(s)	1010
RsaI	GTAC	3 cut(s)	344, 1724, 1816
RsaNI	GTAC	3 cut(s)	343, 1723, 1815
RseI	CAYNNNNRTG	2 cut(s)	849, 1306
SacI	GAGCTC	1 cut(s)	865
SaqAI	TTAA	8 cut(s)	675, 749, 1050, 1131, 1194, 1506, 1580, 1620
SatI	GCNGC	8 cut(s)	204, 334, 420, 694, 709, 759, 897, 1008
Sau3AI	GATC	6 cut(s)	108, 280, 370, 435, 1124, 1150
Sau96I	GGNCC	8 cut(s)	4, 269, 562, 719, 835, 1061, 1253, 1756
SchI	GAGTC	5 cut(s)	305, 315, 596, 1217, 1420
ScrFI	CCNGG	2 cut(s)	723, 763
SduI	GDGCHC	1 cut(s)	865
SfaNI	GCATC	7 cut(s)	307, 484, 1008, 1288, 1385, 1738, 1769
SfcI	CTRYAG	3 cut(s)	897, 1005, 1386
Sfr274I	CTCGAG	1 cut(s)	1481
SinI	GGWCC	5 cut(s)	4, 562, 719, 1061, 1756
SlaI	CTCGAG	1 cut(s)	1481
SmiMI	CAYNNNNRTG	2 cut(s)	849, 1306
SmlI	CTYRAG	5 cut(s)	1193, 1222, 1380, 1481, 1619
SmoI	CTYRAG	5 cut(s)	1193, 1222, 1380, 1481, 1619
SsiI	CCGC	7 cut(s)	204, 232, 247, 334, 361, 419, 759
SspMI	CTAG	7 cut(s)	534, 543, 705, 866, 965, 1709, 1760
SstI	GAGCTC	1 cut(s)	865
StyD4I	CCNGG	2 cut(s)	721, 761
StyI	CCWWGG	3 cut(s)	565, 1654, 1752
TaaI	ACNGT	7 cut(s)	277, 671, 883, 1086, 1308, 1390, 1399
TaiI	ACGT	3 cut(s)	243, 821, 834
TaqI	TCGA	9 cut(s)	279, 285, 354, 434, 1153, 1206, 1237, 1482, 1746
TaqII	GACCGA	1 cut(s)	451
TatI	WGTACW	2 cut(s)	342, 1814
TauI	GCSGC	4 cut(s)	206, 336, 422, 761
TfiI	GAWTC	1 cut(s)	255
Tru1I	TTAA	8 cut(s)	675, 749, 1050, 1131, 1194, 1506, 1580, 1620
Tru9I	TTAA	8 cut(s)	675, 749, 1050, 1131, 1194, 1506, 1580, 1620
TscAI	CASTG	3 cut(s)	105, 1575, 1705
TseFI	GTSAC	4 cut(s)	50, 181, 954, 1524
TseI	GCWGC	4 cut(s)	693, 708, 896, 1007
Tsp45I	GTSAC	4 cut(s)	50, 181, 954, 1524
TspDTI	ATGAA	5 cut(s)	772, 996, 1088, 1360, 1429
TspGWI	ACGGA	1 cut(s)	318
TspRI	CASTG	3 cut(s)	105, 1575, 1705
Vha464I	CTTAAG	2 cut(s)	1193, 1619
VpaK11BI	GGWCC	5 cut(s)	4, 562, 719, 1061, 1756
XapI	RAATTY	1 cut(s)	1808
XbaI	TCTAGA	1 cut(s)	964
XceI	RCATGY	2 cut(s)	390, 1034
XhoI	CTCGAG	1 cut(s)	1481
XspI	CTAG	7 cut(s)	534, 543, 705, 866, 965, 1709, 1760
Zsp2I	ATGCAT	1 cut(s)	1303

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.