MD08G1057500.v1.1

Protein POLAR LOCALIZATION DURING ASYMMETRIC DIVISION AND

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010398
Physical Location & Seq
Reverse (-)
4475732 .. 4477976
2245 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1057500.v1.1.491

Sequence Viewer

Length: 1245 bp
ATGAGGAAGAAGAAGAAGGACGATCGCGAACCCTTCTTTAAAACACTAAACATTCTCTCCCCTTTCCGTTGCTCCAAGGCCCGCCACATCCGCATCGCCGACATACTCGTTGATGAAGAATTGGGATCCGAAACCGACGGCTACGATTGCTCCGGAGACTTCAATATGGAGAGAGACCCACACCGCCTCCCCGAGGACGACGGATTCATGGCCTTACAGTGCTCGTCCCCGCTTTATATTATTTCCCGATGGTTGTCGACTCTGAAACGAGGCAAACGGAGGCGATGCGGCGTCGTACGGAGCCAGAAGAGACCGAAAGAGGTCGCGAAGACGGAGAGGGAGACTGGCGACGACGAGTCAACGCACGCCACCGGTTCGACGAAGGGATTGAACGGCGGTGGCGGGGAAACAGAATCAAGACAGATTGTGAAGGATACGAGCTCATTCAATTTGGGAGTTGCGTGCGGTTTGGTGTATCTGATCGTCGCCGGTAAAAATGAACTTGCAAAGACGGCGGAGTTGCGGGCGGAGATGGAGCAGTTGCTTCAAAATGCAAAAGAGGAACTGCAGAGCAAGAATTCGCGTTTCGGTTCCAGGCCGGTGGAGTCGAATGAGATGAATTTTGCTTCTTCCACCACCGATCTCCAACAGGCTTCGAGTTCGAGCTCTGAGAGCCGGTTTTCACTTCAATCTGGCCTGGTCCGTGAAGTGTGCTCGGAATTTGTTAGAAATGGAAGAGGACAAGGAGAAAGGGATGAATGCGTGGAGGGAATGGATCAACTCGAGGCGGAGCTTGAATCGGAGTTGGAACGATTGCAGCTGCATTTGGAATTGGATTCGGAAAATCATTCATCCAATTCTAACTACACGCAGCAGCAAAGATTAAACGCTGTCCAAGACACAGCTCATGGGACAGGCGACGATACCTCAGATTTTGGAGAAGAAATCGACGCACATGGAGCAGAACCAGCAGATTATAACGAAATCCCCTGCGACTACGGAGTTCCTGCACTCGAACTGGAGAGAAGGCTGCACGAACTGCTGGAAGCGAGGCAGGAAGAACGGATAAAGGAGCTTGAAGTTGCATTGGAGTACACGAAGCGCAAGCTTCATGAGAAGGAAATGGAGGTTTCGTGGTGGAGACAAAATGCGTCTGCAGCACTCACATCGCACCGCCATCAAGATCCTTCATCCTCCGGTCCTTCCCAGCACAGTTCGGAGAGTACATTTCACTTGTTTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008356 GO:0009987 GO:0044424 GO:0044464 GO:0051301 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

415

Amino Acids

46.7

Weight (kDa)

5.22

Isoelectric Point (pI)

58.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31805 AT5G10890 AT5G10890
fragaria_vesca FvH4_2g41090
malus_domestica MD08G1057500.v1.1 MD15G1039900.v1.1
prunus_persica Prupe.1G393300_v2.0.a1
pyrus_communis pycom08g04700 pycom15g03730 pycom15g03740
rosa_chinensis RchiOBHm_Chr6g0299451
rosa_laevigata RLG00000011430
rosa_multiflora Rmu_sc0017526.1_g000008 Rmu_sc0037301.1_g000003
rosa_roxburghii Rroxscaffold_7G00168670
rosa_rugosa Rorug06G0288400
rosa_samantha Rh6AG400200 Rh6BG408800 Rh6CG414300 Rh6DG401300
rosa_wichuraiana Rw6G035030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 978
AccI GTMKAC 1 cut(s) 257
AccII CGCG 3 cut(s) 27, 326, 583
AccIII TCCGGA 1 cut(s) 152
AclWI GGATC 4 cut(s) 120, 133, 783, 1178
AcsI RAATTY 3 cut(s) 577, 619, 719
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 3 cut(s) 297, 1094, 1225
AfiI CCNNNNNNNGG 1 cut(s) 193
AgeI ACCGGT 1 cut(s) 371
AgsI TTSAA 7 cut(s) 163, 391, 448, 548, 689, 797, 1079
AhdI GACNNNNNGTC 1 cut(s) 355
AjnI CCWGG 2 cut(s) 593, 696
AluBI AGCT 7 cut(s) 441, 666, 793, 820, 905, 1075, 1108
AluI AGCT 7 cut(s) 441, 666, 793, 820, 905, 1075, 1108
Alw21I GWGCWC 4 cut(s) 224, 443, 668, 716
Alw26I GTCTC 5 cut(s) 150, 168, 304, 335, 1135
AlwI GGATC 4 cut(s) 120, 133, 783, 1178
Ama87I CYCGRG 2 cut(s) 191, 782
Aor13HI TCCGGA 1 cut(s) 152
AoxI GGCC 4 cut(s) 78, 210, 596, 694
ApeKI GCWGC 6 cut(s) 817, 820, 871, 874, 1030, 1157
ApoI RAATTY 3 cut(s) 577, 619, 719
AsiGI ACCGGT 1 cut(s) 371
AspLEI GCGC 1 cut(s) 1104
AspS9I GGNCC 3 cut(s) 79, 700, 1199
AvaI CYCGRG 2 cut(s) 191, 782
AvaII GGWCC 2 cut(s) 700, 1199
BamHI GGATCC 1 cut(s) 125
BanII GRGCYC 2 cut(s) 443, 668
BbsI GAAGAC 1 cut(s) 335
Bbv12I GWGCWC 4 cut(s) 224, 443, 668, 716
BbvI GCAGC 6 cut(s) 807, 829, 883, 886, 1017, 1169
BccI CCATC 3 cut(s) 243, 526, 1185
BceAI ACGGC 3 cut(s) 154, 409, 528
BcgI CGANNNNNNTGC 2 cut(s) 1149, 1183
BciT130I CCWGG 2 cut(s) 595, 698
BciVI GTATCC 1 cut(s) 427
BcoDI GTCTC 5 cut(s) 150, 168, 304, 335, 1135
BfmI CTRYAG 2 cut(s) 566, 1155
BfuI GTATCC 1 cut(s) 427
BisI GCNGC 7 cut(s) 289, 818, 821, 872, 875, 1031, 1158
BlsI GCNGC 7 cut(s) 290, 819, 822, 873, 876, 1032, 1159
Bme1390I CCNGG 2 cut(s) 595, 698
Bme18I GGWCC 2 cut(s) 700, 1199
BmeRI GACNNNNNGTC 1 cut(s) 355
BmeT110I CYCGRG 2 cut(s) 191, 782
BmgT120I GGNCC 3 cut(s) 79, 700, 1199
BmiI GGNNCC 3 cut(s) 127, 302, 592
BmrFI CCNGG 2 cut(s) 595, 698
BmsI GCATC 2 cut(s) 102, 275
BpiI GAAGAC 1 cut(s) 335
BpmI CTGGAG 1 cut(s) 1040
BsaHI GRCGYC 1 cut(s) 291
BsaI GGTCTC 2 cut(s) 168, 304
BsaJI CCNNGG 2 cut(s) 75, 192
BsaWI WCCGGW 3 cut(s) 152, 371, 1196
BsaXI ACNNNNNCTCC 8 cut(s) 41, 71, 134, 164, 171, 201, 1118, 1148
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse118I RCCGGY 4 cut(s) 371, 488, 598, 675
Bse1I ACTGG 2 cut(s) 349, 1023
BseAI TCCGGA 1 cut(s) 152
BseBI CCWGG 2 cut(s) 595, 698
BseDI CCNNGG 2 cut(s) 75, 192
BseGI GGATG 4 cut(s) 87, 760, 851, 1190
BseLI CCNNNNNNNGG 1 cut(s) 193
BseMII CTCAG 2 cut(s) 660, 942
BseNI ACTGG 2 cut(s) 349, 1023
BseXI GCAGC 6 cut(s) 807, 829, 883, 886, 1017, 1169
BseYI CCCAGC 1 cut(s) 1206
BsgI GTGCAG 2 cut(s) 993, 1016
Bsh1236I CGCG 3 cut(s) 27, 326, 583
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 4 cut(s) 80, 212, 598, 696
BshTI ACCGGT 1 cut(s) 371
BsiEI CGRYCG 1 cut(s) 25
BsiHKAI GWGCWC 4 cut(s) 224, 443, 668, 716
BsiHKCI CYCGRG 2 cut(s) 191, 782
BsiSI CCGG 6 cut(s) 153, 372, 489, 599, 676, 1197
BsiWI CGTACG 1 cut(s) 295
BslFI GGGAC 2 cut(s) 211, 925
BslI CCNNNNNNNGG 1 cut(s) 193
BsmAI GTCTC 5 cut(s) 150, 168, 304, 335, 1135
BsmFI GGGAC 2 cut(s) 211, 925
BsmI GAATGC 1 cut(s) 764
BsnI GGCC 4 cut(s) 80, 212, 598, 696
Bso31I GGTCTC 2 cut(s) 168, 304
BsoBI CYCGRG 2 cut(s) 191, 782
Bsp1286I GDGCHC 4 cut(s) 224, 443, 668, 716
Bsp13I TCCGGA 1 cut(s) 152
Bsp143I GATC 6 cut(s) 22, 125, 480, 640, 775, 1183
Bsp68I TCGCGA 2 cut(s) 27, 326
BspANI GGCC 4 cut(s) 80, 212, 598, 696
BspCNI CTCAG 2 cut(s) 661, 941
BspEI TCCGGA 1 cut(s) 152
BspFNI CGCG 3 cut(s) 27, 326, 583
BspHI TCATGA 1 cut(s) 1111
BspLI GGNNCC 3 cut(s) 127, 302, 592
BspMAI CTGCAG 2 cut(s) 570, 1159
BspPI GGATC 4 cut(s) 120, 133, 783, 1178
BspTNI GGTCTC 2 cut(s) 168, 304
BsrFI RCCGGY 4 cut(s) 371, 488, 598, 675
BsrI ACTGG 2 cut(s) 349, 1023
BssAI RCCGGY 4 cut(s) 371, 488, 598, 675
BssECI CCNNGG 2 cut(s) 75, 192
BssMI GATC 6 cut(s) 22, 125, 480, 640, 775, 1183
BssNI GRCGYC 1 cut(s) 291
BssT1I CCWWGG 1 cut(s) 75
Bst2UI CCWGG 2 cut(s) 595, 698
Bst4CI ACNGT 2 cut(s) 219, 1214
Bst6I CTCTTC 2 cut(s) 302, 730
BstACI GRCGYC 1 cut(s) 291
BstAPI GCANNNNNTGC 1 cut(s) 1039
BstC8I GCNNGC 5 cut(s) 82, 366, 463, 525, 1106
BstDEI CTNAG 2 cut(s) 669, 928
BstENI CCTNNNNNAGG 1 cut(s) 191
BstF5I GGATG 4 cut(s) 87, 760, 851, 1190
BstFNI CGCG 3 cut(s) 27, 326, 583
BstHHI GCGC 1 cut(s) 1104
BstKTI GATC 6 cut(s) 25, 128, 483, 643, 778, 1186
BstMAI GTCTC 5 cut(s) 150, 168, 304, 335, 1135
BstMBI GATC 6 cut(s) 22, 125, 480, 640, 775, 1183
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 8 cut(s) 90, 147, 512, 672, 959, 968, 1039, 1157
BstNI CCWGG 2 cut(s) 595, 698
BstSCI CCNGG 2 cut(s) 593, 696
BstSFI CTRYAG 2 cut(s) 566, 1155
BstUI CGCG 3 cut(s) 27, 326, 583
BstV1I GCAGC 6 cut(s) 807, 829, 883, 886, 1017, 1169
BstV2I GAAGAC 1 cut(s) 335
BstX2I RGATCY 2 cut(s) 125, 1183
BstXI CCANNNNNNTGG 1 cut(s) 601
BstYI RGATCY 2 cut(s) 125, 1183
BsuI GTATCC 1 cut(s) 427
BsuRI GGCC 4 cut(s) 80, 212, 598, 696
BtgZI GCGATG 3 cut(s) 79, 298, 1152
BtsCI GGATG 4 cut(s) 87, 760, 851, 1190
BtsIMutI CAGTG 1 cut(s) 224
BtuMI TCGCGA 2 cut(s) 27, 326
Cac8I GCNNGC 5 cut(s) 82, 366, 463, 525, 1106
CciI TCATGA 1 cut(s) 1111
CfoI GCGC 1 cut(s) 1104
Cfr10I RCCGGY 4 cut(s) 371, 488, 598, 675
Cfr13I GGNCC 3 cut(s) 79, 700, 1199
CseI GACGC 3 cut(s) 280, 959, 1140
Csp6I GTAC 3 cut(s) 296, 1093, 1224
CspAI ACCGGT 1 cut(s) 371
CviAII CATG 4 cut(s) 208, 908, 956, 1112
CviQI GTAC 3 cut(s) 296, 1093, 1224
DdeI CTNAG 2 cut(s) 669, 928
DpnI GATC 6 cut(s) 24, 127, 482, 642, 777, 1185
DpnII GATC 6 cut(s) 22, 125, 480, 640, 775, 1183
DraI TTTAAA 1 cut(s) 40
DriI GACNNNNNGTC 1 cut(s) 355
Eam1104I CTCTTC 2 cut(s) 302, 730
Eam1105I GACNNNNNGTC 1 cut(s) 355
EarI CTCTTC 2 cut(s) 302, 730
EciI GGCGGA 3 cut(s) 530, 542, 803
Ecl136II GAGCTC 2 cut(s) 441, 666
Eco130I CCWWGG 1 cut(s) 75
Eco24I GRGCYC 2 cut(s) 443, 668
Eco31I GGTCTC 2 cut(s) 168, 304
Eco47I GGWCC 2 cut(s) 700, 1199
Eco53kI GAGCTC 2 cut(s) 441, 666
Eco88I CYCGRG 2 cut(s) 191, 782
EcoICRI GAGCTC 2 cut(s) 441, 666
EcoNI CCTNNNNNAGG 1 cut(s) 191
EcoRI GAATTC 1 cut(s) 577
EcoRII CCWGG 2 cut(s) 593, 696
EcoT14I CCWWGG 1 cut(s) 75
EcoT38I GRGCYC 2 cut(s) 443, 668
ErhI CCWWGG 1 cut(s) 75
FaeI CATG 4 cut(s) 211, 911, 959, 1115
FaiI YATR 8 cut(s) 104, 167, 209, 237, 909, 957, 978, 1113
FaqI GGGAC 2 cut(s) 211, 925
FatI CATG 4 cut(s) 207, 907, 955, 1111
FauI CCCGC 4 cut(s) 89, 237, 395, 516
FblI GTMKAC 1 cut(s) 257
Fnu4HI GCNGC 7 cut(s) 289, 818, 821, 872, 875, 1031, 1158
FokI GGATG 4 cut(s) 74, 767, 838, 1177
FriOI GRGCYC 2 cut(s) 443, 668
Fsp4HI GCNGC 7 cut(s) 289, 818, 821, 872, 875, 1031, 1158
GlaI GCGC 1 cut(s) 1103
GluI GCNGC 7 cut(s) 289, 818, 821, 872, 875, 1031, 1158
GsaI CCCAGC 1 cut(s) 1210
GsuI CTGGAG 1 cut(s) 1040
HaeIII GGCC 4 cut(s) 80, 212, 598, 696
HapII CCGG 6 cut(s) 153, 372, 489, 599, 676, 1197
HgaI GACGC 3 cut(s) 280, 959, 1140
HhaI GCGC 1 cut(s) 1104
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 4 cut(s) 211, 911, 959, 1115
Hin6I GCGC 1 cut(s) 1102
HinP1I GCGC 1 cut(s) 1102
HincII GTYRAC 2 cut(s) 258, 360
HindII GTYRAC 2 cut(s) 258, 360
HindIII AAGCTT 1 cut(s) 1106
HinfI GANTC 7 cut(s) 204, 259, 356, 413, 605, 797, 836
HpaII CCGG 6 cut(s) 153, 372, 489, 599, 676, 1197
Hpy166II GTNNAC 3 cut(s) 258, 360, 1095
Hpy188I TCNGA 9 cut(s) 130, 264, 480, 670, 718, 802, 841, 931, 1219
Hpy188III TCNNGA 7 cut(s) 26, 153, 246, 325, 417, 1112, 1181
Hpy8I GTNNAC 3 cut(s) 258, 360, 1095
Hpy99I CGWCG 9 cut(s) 140, 203, 296, 353, 356, 382, 488, 923, 953
HpyAV CCTTC 8 cut(s) 10, 43, 376, 424, 1020, 1111, 1197, 1212
HpyCH4III ACNGT 2 cut(s) 219, 1214
HpyCH4V TGCA 9 cut(s) 506, 554, 568, 817, 823, 1010, 1033, 1085, 1157
HpyF10VI GCNNNNNNNGC 8 cut(s) 90, 147, 512, 672, 959, 968, 1039, 1157
HpyF3I CTNAG 2 cut(s) 669, 928
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 4 cut(s) 211, 911, 959, 1115
HspAI GCGC 1 cut(s) 1102
Kpn2I TCCGGA 1 cut(s) 152
Kzo9I GATC 6 cut(s) 22, 125, 480, 640, 775, 1183
LmnI GCTCC 7 cut(s) 77, 155, 300, 535, 790, 959, 1072
Lsp1109I GCAGC 6 cut(s) 807, 829, 883, 886, 1017, 1169
LweI GCATC 2 cut(s) 102, 275
MalI GATC 6 cut(s) 24, 127, 482, 642, 777, 1185
MboI GATC 6 cut(s) 22, 125, 480, 640, 775, 1183
MflI RGATCY 2 cut(s) 125, 1183
MhlI GDGCHC 4 cut(s) 224, 443, 668, 716
MluCI AATT 7 cut(s) 119, 448, 577, 619, 719, 830, 856
MlyI GAGTC 3 cut(s) 253, 365, 614
MmeI TCCRAC 2 cut(s) 670, 786
MroI TCCGGA 1 cut(s) 152
MseI TTAA 2 cut(s) 39, 884
MspA1I CMGCKG 1 cut(s) 820
MspI CCGG 6 cut(s) 153, 372, 489, 599, 676, 1197
MspR9I CCNGG 2 cut(s) 595, 698
Mva1269I GAATGC 1 cut(s) 764
MvaI CCWGG 2 cut(s) 595, 698
MvnI CGCG 3 cut(s) 27, 326, 583
MwoI GCNNNNNNNGC 8 cut(s) 90, 147, 512, 672, 959, 968, 1039, 1157
NdeII GATC 6 cut(s) 22, 125, 480, 640, 775, 1183
NlaIII CATG 4 cut(s) 211, 911, 959, 1115
NlaIV GGNNCC 3 cut(s) 127, 302, 592
NruI TCGCGA 2 cut(s) 27, 326
PaeR7I CTCGAG 1 cut(s) 782
PagI TCATGA 1 cut(s) 1111
PctI GAATGC 1 cut(s) 764
PfeI GAWTC 4 cut(s) 204, 413, 797, 836
Pfl23II CGTACG 1 cut(s) 295
PinAI ACCGGT 1 cut(s) 371
PkrI GCNGC 7 cut(s) 290, 819, 822, 873, 876, 1032, 1159
Ple19I CGATCG 1 cut(s) 25
PleI GAGTC 3 cut(s) 253, 364, 613
PpsI GAGTC 3 cut(s) 253, 364, 613
PsiI TTATAA 1 cut(s) 978
Psp124BI GAGCTC 2 cut(s) 443, 668
Psp6I CCWGG 2 cut(s) 593, 696
PspFI CCCAGC 1 cut(s) 1206
PspGI CCWGG 2 cut(s) 593, 696
PspLI CGTACG 1 cut(s) 295
PspN4I GGNNCC 3 cut(s) 127, 302, 592
PspPI GGNCC 3 cut(s) 79, 700, 1199
PspXI VCTCGAGB 1 cut(s) 782
PstI CTGCAG 2 cut(s) 570, 1159
PsuI RGATCY 2 cut(s) 125, 1183
PvuI CGATCG 1 cut(s) 25
PvuII CAGCTG 1 cut(s) 820
RruI TCGCGA 2 cut(s) 27, 326
RsaI GTAC 3 cut(s) 297, 1094, 1225
RsaNI GTAC 3 cut(s) 296, 1093, 1224
SacI GAGCTC 2 cut(s) 443, 668
SalI GTCGAC 1 cut(s) 256
SaqAI TTAA 2 cut(s) 39, 884
SatI GCNGC 7 cut(s) 289, 818, 821, 872, 875, 1031, 1158
Sau3AI GATC 6 cut(s) 22, 125, 480, 640, 775, 1183
Sau96I GGNCC 3 cut(s) 79, 700, 1199
SchI GAGTC 3 cut(s) 253, 365, 614
ScrFI CCNGG 2 cut(s) 595, 698
SduI GDGCHC 4 cut(s) 224, 443, 668, 716
SfaNI GCATC 2 cut(s) 102, 275
SfcI CTRYAG 2 cut(s) 566, 1155
Sfr274I CTCGAG 1 cut(s) 782
SinI GGWCC 2 cut(s) 700, 1199
SlaI CTCGAG 1 cut(s) 782
SmlI CTYRAG 1 cut(s) 782
SmoI CTYRAG 1 cut(s) 782
Sse9I AATT 7 cut(s) 119, 448, 577, 619, 719, 830, 856
SstI GAGCTC 2 cut(s) 443, 668
StyD4I CCNGG 2 cut(s) 593, 696
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 2 cut(s) 219, 1214
TaqI TCGA 8 cut(s) 257, 377, 608, 656, 662, 783, 948, 1014
TaqII GACCGA 1 cut(s) 328
TasI AATT 7 cut(s) 119, 448, 577, 619, 719, 830, 856
TatI WGTACW 2 cut(s) 1092, 1223
TauI GCSGC 1 cut(s) 291
TfiI GAWTC 4 cut(s) 204, 413, 797, 836
Tru1I TTAA 2 cut(s) 39, 884
Tru9I TTAA 2 cut(s) 39, 884
TscAI CASTG 1 cut(s) 224
TseI GCWGC 6 cut(s) 817, 820, 871, 874, 1030, 1157
TspDTI ATGAA 8 cut(s) 129, 196, 513, 632, 771, 840, 1100, 1179
TspGWI ACGGA 8 cut(s) 56, 216, 292, 313, 347, 692, 1014, 1078
TspRI CASTG 1 cut(s) 224
VpaK11BI GGWCC 2 cut(s) 700, 1199
XagI CCTNNNNNAGG 1 cut(s) 191
XapI RAATTY 3 cut(s) 577, 619, 719
XhoI CTCGAG 1 cut(s) 782
XmiI GTMKAC 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.