pycom08g04700

Protein POLAR LOCALIZATION DURING ASYMMETRIC DIVISION AND

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0010398
Physical Location & Seq
Reverse (-)
3549338 .. 3550930
1593 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g04700.4

Sequence Viewer

Length: 1281 bp
ATGAGGAAGAAGAAGAAGGACGATCACGATCCCTTCTTTAAAACCCTAAACATTCTCTGCCCTTTCCGTTACTCCAAGACCCGCCACATCCGCATCGCGGACATACTCGTTGATGAAGAATTGGGATCCGAAACCGGCGGCTACGATTGCGCCGGAGACTTCAATATGGATAGAGACCCACAACGCCTCCCCGAGGACGACGGATTCACGGCCTTACAGTGCTCGTCCCCGCTTTATATTATTTCCCGATGGTTGTCGACTCTGAAGCGAGGCAAACGGAGGCGATGCGGCGTCGTATGGAGCCAGAAGAGACCGAAAGAGGTCGCGAAGACGGAGAGGGAGACTGGCGACGACGAGTCAACGCACGTCACCGGTTCGACGAATGGATTGAACGGCGGTGGCGGGGAAACAGAATCAAGACAGATTGTGAAGGATACGAGCTCATTCAATTTGGGAGTTGCGTGCGGTTTGGTGTATCTGATCGTCACCGGTAAAAATGAACTTGCAAAGACGGCGGAGTTGCGGGCGGAGATGGAGCAGTTGCTTCAAAATGCAAAAGAGGAAAAGCAGAGCAAGAATTCGCGTTTCGGTTCCAGGGCGGTGGAGTCGAATGAGATGAATTTTGCTTCTTCCACCACCGATCTCCAACAGGCTTCGAGTTCGAGCTCTGGGAGCCGGTTTTCACTTCAATCTGGCCTGGTCCGTGAAGTGTGCTCGGAATTTGTTAGAAATGGAAGAGGAGAAGGAGAAAGGGATGAATGCGTGGAGGGAATGGATCAACTCGAGGCGGAGCTTGAAGCGGAGTTGGAACGATTGCAGCTGCATTTGGAATTGGATTCGGAAAATCATTCATCCAATTCTAACTACACGCAGCAGCAAAGATTAAACGCTGTCCAAGACACAGCTCATGGAACGGGCGATGATATCTCAGATTTTGTAGAAGAAATCGACGCGCATGGAGCAGAACCAGCAGATTATAACGAAATCCCCTGTGACTACGGAGTTCCTGCACTCGAACTGGAGAGAAGGCTGCACGAACTGCTGGAAGCGAGGCAGGAAGAACGGATAAAGGAACTTGAAGTTGCGTTGGAGTACACAAAGCGCAAGCTTCATGAGAAGGAAATGGAGGCTTCGTGGTGGAGACAAAATGCATCCGCAGCACTCACATCGCACCACCATCAAGATCCTTCATCGTCGGGTGCTTCACAGCACAGTTCGGAGAGTACATTTCACTTGTTTAGAAACAGTAAGGATACCGAGGGCATTAGTAGCAAAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008356 GO:0009987 GO:0044424 GO:0044464 GO:0051301 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

427

Amino Acids

47.81

Weight (kDa)

5.16

Isoelectric Point (pI)

51.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31805 AT5G10890 AT5G10890
fragaria_vesca FvH4_2g41090
malus_domestica MD08G1057500.v1.1 MD15G1039900.v1.1
prunus_persica Prupe.1G393300_v2.0.a1
pyrus_communis pycom08g04700 pycom15g03730 pycom15g03740
rosa_chinensis RchiOBHm_Chr6g0299451
rosa_laevigata RLG00000011430
rosa_multiflora Rmu_sc0017526.1_g000008 Rmu_sc0037301.1_g000003
rosa_roxburghii Rroxscaffold_7G00168670
rosa_rugosa Rorug06G0288400
rosa_samantha Rh6AG400200 Rh6BG408800 Rh6CG414300 Rh6DG401300
rosa_wichuraiana Rw6G035030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 978
AccI GTMKAC 1 cut(s) 257
AccII CGCG 4 cut(s) 98, 326, 583, 953
AclWI GGATC 5 cut(s) 23, 120, 133, 783, 1178
AcsI RAATTY 3 cut(s) 577, 619, 719
AcuI CTGAAG 1 cut(s) 284
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 2 cut(s) 1094, 1225
AfiI CCNNNNNNNGG 2 cut(s) 97, 193
AgeI ACCGGT 2 cut(s) 371, 488
AgsI TTSAA 7 cut(s) 163, 391, 448, 548, 689, 797, 1079
AhdI GACNNNNNGTC 1 cut(s) 355
AjiI CACGTC 1 cut(s) 367
AjnI CCWGG 2 cut(s) 593, 696
AluBI AGCT 6 cut(s) 441, 666, 793, 820, 905, 1108
AluI AGCT 6 cut(s) 441, 666, 793, 820, 905, 1108
Alw21I GWGCWC 4 cut(s) 224, 443, 668, 716
Alw26I GTCTC 5 cut(s) 150, 168, 304, 335, 1135
AlwI GGATC 5 cut(s) 23, 120, 133, 783, 1178
Ama87I CYCGRG 2 cut(s) 191, 782
AoxI GGCC 2 cut(s) 210, 694
ApeKI GCWGC 6 cut(s) 817, 820, 871, 874, 1030, 1157
ApoI RAATTY 3 cut(s) 577, 619, 719
AsiGI ACCGGT 2 cut(s) 371, 488
AspLEI GCGC 3 cut(s) 152, 955, 1104
AspS9I GGNCC 1 cut(s) 700
AsuHPI GGTGA 2 cut(s) 361, 478
AvaI CYCGRG 2 cut(s) 191, 782
AvaII GGWCC 1 cut(s) 700
BamHI GGATCC 1 cut(s) 125
BanII GRGCYC 2 cut(s) 443, 668
BbsI GAAGAC 1 cut(s) 335
Bbv12I GWGCWC 4 cut(s) 224, 443, 668, 716
BbvI GCAGC 6 cut(s) 807, 829, 883, 886, 1017, 1169
BccI CCATC 3 cut(s) 243, 526, 1185
BceAI ACGGC 3 cut(s) 225, 409, 528
BcgI CGANNNNNNTGC 2 cut(s) 1149, 1183
BciT130I CCWGG 2 cut(s) 595, 698
BciVI GTATCC 2 cut(s) 427, 1246
BcoDI GTCTC 5 cut(s) 150, 168, 304, 335, 1135
BfuI GTATCC 2 cut(s) 427, 1246
BisI GCNGC 8 cut(s) 139, 289, 818, 821, 872, 875, 1031, 1158
BlsI GCNGC 8 cut(s) 140, 290, 819, 822, 873, 876, 1032, 1159
Bme1390I CCNGG 2 cut(s) 595, 698
Bme18I GGWCC 1 cut(s) 700
BmeRI GACNNNNNGTC 1 cut(s) 355
BmeT110I CYCGRG 2 cut(s) 191, 782
BmgBI CACGTC 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 700
BmiI GGNNCC 4 cut(s) 127, 302, 592, 674
BmrFI CCNGG 2 cut(s) 595, 698
BmsI GCATC 3 cut(s) 102, 275, 1160
BpiI GAAGAC 1 cut(s) 335
BpmI CTGGAG 1 cut(s) 1040
BsaBI GATNNNNATC 1 cut(s) 27
BsaHI GRCGYC 1 cut(s) 291
BsaI GGTCTC 2 cut(s) 168, 304
BsaJI CCNNGG 3 cut(s) 192, 594, 1257
BsaWI WCCGGW 2 cut(s) 371, 488
BsaXI ACNNNNNCTCC 4 cut(s) 171, 201, 1118, 1148
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 193
Bse118I RCCGGY 4 cut(s) 134, 371, 488, 675
Bse1I ACTGG 2 cut(s) 349, 1023
Bse8I GATNNNNATC 1 cut(s) 27
BseBI CCWGG 2 cut(s) 595, 698
BseDI CCNNGG 3 cut(s) 192, 594, 1257
BseGI GGATG 4 cut(s) 87, 760, 851, 1151
BseJI GATNNNNATC 1 cut(s) 27
BseLI CCNNNNNNNGG 2 cut(s) 97, 193
BseMII CTCAG 1 cut(s) 942
BseNI ACTGG 2 cut(s) 349, 1023
BseRI GAGGAG 1 cut(s) 753
BseXI GCAGC 6 cut(s) 807, 829, 883, 886, 1017, 1169
BsgI GTGCAG 2 cut(s) 993, 1016
Bsh1236I CGCG 4 cut(s) 98, 326, 583, 953
BshFI GGCC 2 cut(s) 212, 696
BshTI ACCGGT 2 cut(s) 371, 488
BsiHKAI GWGCWC 4 cut(s) 224, 443, 668, 716
BsiHKCI CYCGRG 2 cut(s) 191, 782
BsiSI CCGG 5 cut(s) 135, 153, 372, 489, 676
BslFI GGGAC 1 cut(s) 211
BslI CCNNNNNNNGG 2 cut(s) 97, 193
BsmAI GTCTC 5 cut(s) 150, 168, 304, 335, 1135
BsmFI GGGAC 1 cut(s) 211
BsmI GAATGC 1 cut(s) 764
BsnI GGCC 2 cut(s) 212, 696
Bso31I GGTCTC 2 cut(s) 168, 304
BsoBI CYCGRG 2 cut(s) 191, 782
Bsp1286I GDGCHC 4 cut(s) 224, 443, 668, 716
Bsp143I GATC 7 cut(s) 22, 28, 125, 480, 640, 775, 1183
Bsp68I TCGCGA 1 cut(s) 326
BspANI GGCC 2 cut(s) 212, 696
BspCNI CTCAG 1 cut(s) 941
BspFNI CGCG 4 cut(s) 98, 326, 583, 953
BspHI TCATGA 1 cut(s) 1111
BspLI GGNNCC 4 cut(s) 127, 302, 592, 674
BspPI GGATC 5 cut(s) 23, 120, 133, 783, 1178
BspTNI GGTCTC 2 cut(s) 168, 304
BsrFI RCCGGY 4 cut(s) 134, 371, 488, 675
BsrI ACTGG 2 cut(s) 349, 1023
BssAI RCCGGY 4 cut(s) 134, 371, 488, 675
BssECI CCNNGG 3 cut(s) 192, 594, 1257
BssMI GATC 7 cut(s) 22, 28, 125, 480, 640, 775, 1183
BssNI GRCGYC 1 cut(s) 291
Bst2UI CCWGG 2 cut(s) 595, 698
Bst4CI ACNGT 3 cut(s) 219, 1214, 1247
Bst6I CTCTTC 2 cut(s) 302, 730
BstACI GRCGYC 1 cut(s) 291
BstAPI GCANNNNNTGC 1 cut(s) 1039
BstC8I GCNNGC 3 cut(s) 463, 525, 1106
BstDEI CTNAG 1 cut(s) 928
BstENI CCTNNNNNAGG 1 cut(s) 191
BstF5I GGATG 4 cut(s) 87, 760, 851, 1151
BstFNI CGCG 4 cut(s) 98, 326, 583, 953
BstHHI GCGC 3 cut(s) 152, 955, 1104
BstKTI GATC 7 cut(s) 25, 31, 128, 483, 643, 778, 1186
BstMAI GTCTC 5 cut(s) 150, 168, 304, 335, 1135
BstMBI GATC 7 cut(s) 22, 28, 125, 480, 640, 775, 1183
BstMWI GCNNNNNNNGC 9 cut(s) 90, 147, 512, 672, 959, 968, 1039, 1157, 1269
BstNI CCWGG 2 cut(s) 595, 698
BstSCI CCNGG 2 cut(s) 593, 696
BstUI CGCG 4 cut(s) 98, 326, 583, 953
BstV1I GCAGC 6 cut(s) 807, 829, 883, 886, 1017, 1169
BstV2I GAAGAC 1 cut(s) 335
BstX2I RGATCY 2 cut(s) 125, 1183
BstXI CCANNNNNNTGG 1 cut(s) 601
BstYI RGATCY 2 cut(s) 125, 1183
BsuI GTATCC 2 cut(s) 427, 1246
BsuRI GGCC 2 cut(s) 212, 696
BtgZI GCGATG 4 cut(s) 79, 298, 933, 1152
BtrI CACGTC 1 cut(s) 367
BtsCI GGATG 4 cut(s) 87, 760, 851, 1151
BtsIMutI CAGTG 1 cut(s) 224
BtuMI TCGCGA 1 cut(s) 326
Cac8I GCNNGC 3 cut(s) 463, 525, 1106
CciI TCATGA 1 cut(s) 1111
CfoI GCGC 3 cut(s) 152, 955, 1104
Cfr10I RCCGGY 4 cut(s) 134, 371, 488, 675
Cfr13I GGNCC 1 cut(s) 700
CseI GACGC 2 cut(s) 280, 959
Csp6I GTAC 2 cut(s) 1093, 1224
CspAI ACCGGT 2 cut(s) 371, 488
CviAII CATG 3 cut(s) 908, 956, 1112
CviQI GTAC 2 cut(s) 1093, 1224
DdeI CTNAG 1 cut(s) 928
DpnI GATC 7 cut(s) 24, 30, 127, 482, 642, 777, 1185
DpnII GATC 7 cut(s) 22, 28, 125, 480, 640, 775, 1183
DraI TTTAAA 1 cut(s) 40
DriI GACNNNNNGTC 1 cut(s) 355
Eam1104I CTCTTC 2 cut(s) 302, 730
Eam1105I GACNNNNNGTC 1 cut(s) 355
EarI CTCTTC 2 cut(s) 302, 730
EciI GGCGGA 3 cut(s) 530, 542, 803
Ecl136II GAGCTC 2 cut(s) 441, 666
Eco24I GRGCYC 2 cut(s) 443, 668
Eco31I GGTCTC 2 cut(s) 168, 304
Eco32I GATATC 1 cut(s) 925
Eco47I GGWCC 1 cut(s) 700
Eco53kI GAGCTC 2 cut(s) 441, 666
Eco57I CTGAAG 1 cut(s) 284
Eco88I CYCGRG 2 cut(s) 191, 782
EcoICRI GAGCTC 2 cut(s) 441, 666
EcoNI CCTNNNNNAGG 1 cut(s) 191
EcoRI GAATTC 1 cut(s) 577
EcoRII CCWGG 2 cut(s) 593, 696
EcoRV GATATC 1 cut(s) 925
EcoT22I ATGCAT 1 cut(s) 1153
EcoT38I GRGCYC 2 cut(s) 443, 668
FaeI CATG 3 cut(s) 911, 959, 1115
FaiI YATR 8 cut(s) 104, 167, 237, 298, 909, 957, 978, 1113
FaqI GGGAC 1 cut(s) 211
FatI CATG 3 cut(s) 907, 955, 1111
FauI CCCGC 4 cut(s) 89, 237, 395, 516
FblI GTMKAC 1 cut(s) 257
Fnu4HI GCNGC 8 cut(s) 139, 289, 818, 821, 872, 875, 1031, 1158
FokI GGATG 4 cut(s) 74, 767, 838, 1138
FriOI GRGCYC 2 cut(s) 443, 668
Fsp4HI GCNGC 8 cut(s) 139, 289, 818, 821, 872, 875, 1031, 1158
GlaI GCGC 3 cut(s) 151, 954, 1103
GluI GCNGC 8 cut(s) 139, 289, 818, 821, 872, 875, 1031, 1158
GsuI CTGGAG 1 cut(s) 1040
HaeIII GGCC 2 cut(s) 212, 696
HapII CCGG 5 cut(s) 135, 153, 372, 489, 676
HgaI GACGC 2 cut(s) 280, 959
HhaI GCGC 3 cut(s) 152, 955, 1104
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 3 cut(s) 911, 959, 1115
Hin6I GCGC 3 cut(s) 150, 953, 1102
HinP1I GCGC 3 cut(s) 150, 953, 1102
HincII GTYRAC 2 cut(s) 258, 360
HindII GTYRAC 2 cut(s) 258, 360
HindIII AAGCTT 1 cut(s) 1106
HinfI GANTC 6 cut(s) 204, 259, 356, 413, 605, 836
HpaII CCGG 5 cut(s) 135, 153, 372, 489, 676
HphI GGTGA 2 cut(s) 361, 478
Hpy166II GTNNAC 3 cut(s) 258, 360, 1095
Hpy188I TCNGA 7 cut(s) 130, 264, 480, 718, 841, 931, 1219
Hpy188III TCNNGA 6 cut(s) 26, 246, 325, 417, 1112, 1181
Hpy8I GTNNAC 3 cut(s) 258, 360, 1095
Hpy99I CGWCG 7 cut(s) 203, 296, 353, 356, 382, 953, 1198
HpyAV CCTTC 7 cut(s) 10, 43, 424, 737, 1020, 1111, 1197
HpyCH4III ACNGT 3 cut(s) 219, 1214, 1247
HpyCH4IV ACGT 1 cut(s) 366
HpyCH4V TGCA 7 cut(s) 506, 554, 817, 823, 1010, 1033, 1151
HpyF10VI GCNNNNNNNGC 9 cut(s) 90, 147, 512, 672, 959, 968, 1039, 1157, 1269
HpyF3I CTNAG 1 cut(s) 928
HpySE526I ACGT 1 cut(s) 366
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 3 cut(s) 911, 959, 1115
HspAI GCGC 3 cut(s) 150, 953, 1102
Kzo9I GATC 7 cut(s) 22, 28, 125, 480, 640, 775, 1183
LmnI GCTCC 5 cut(s) 300, 535, 672, 790, 959
Lsp1109I GCAGC 6 cut(s) 807, 829, 883, 886, 1017, 1169
LweI GCATC 3 cut(s) 102, 275, 1160
MaeII ACGT 1 cut(s) 366
MaeIII GTNAC 4 cut(s) 68, 367, 484, 992
MalI GATC 7 cut(s) 24, 30, 127, 482, 642, 777, 1185
MboI GATC 7 cut(s) 22, 28, 125, 480, 640, 775, 1183
MflI RGATCY 2 cut(s) 125, 1183
MhlI GDGCHC 4 cut(s) 224, 443, 668, 716
MluCI AATT 7 cut(s) 119, 448, 577, 619, 719, 830, 856
MlyI GAGTC 3 cut(s) 253, 365, 614
MmeI TCCRAC 3 cut(s) 670, 786, 1068
Mph1103I ATGCAT 1 cut(s) 1153
MseI TTAA 2 cut(s) 39, 884
MspA1I CMGCKG 1 cut(s) 820
MspI CCGG 5 cut(s) 135, 153, 372, 489, 676
MspR9I CCNGG 2 cut(s) 595, 698
Mva1269I GAATGC 1 cut(s) 764
MvaI CCWGG 2 cut(s) 595, 698
MvnI CGCG 4 cut(s) 98, 326, 583, 953
MwoI GCNNNNNNNGC 9 cut(s) 90, 147, 512, 672, 959, 968, 1039, 1157, 1269
NdeII GATC 7 cut(s) 22, 28, 125, 480, 640, 775, 1183
NlaIII CATG 3 cut(s) 911, 959, 1115
NlaIV GGNNCC 4 cut(s) 127, 302, 592, 674
NmuCI GTSAC 3 cut(s) 367, 484, 992
NruI TCGCGA 1 cut(s) 326
NsiI ATGCAT 1 cut(s) 1153
PaeR7I CTCGAG 1 cut(s) 782
PagI TCATGA 1 cut(s) 1111
PctI GAATGC 1 cut(s) 764
PfeI GAWTC 3 cut(s) 204, 413, 836
PinAI ACCGGT 2 cut(s) 371, 488
PkrI GCNGC 8 cut(s) 140, 290, 819, 822, 873, 876, 1032, 1159
PleI GAGTC 3 cut(s) 253, 364, 613
PpsI GAGTC 3 cut(s) 253, 364, 613
PsiI TTATAA 1 cut(s) 978
Psp124BI GAGCTC 2 cut(s) 443, 668
Psp6I CCWGG 2 cut(s) 593, 696
PspGI CCWGG 2 cut(s) 593, 696
PspN4I GGNNCC 4 cut(s) 127, 302, 592, 674
PspPI GGNCC 1 cut(s) 700
PspXI VCTCGAGB 1 cut(s) 782
PsuI RGATCY 2 cut(s) 125, 1183
PvuII CAGCTG 1 cut(s) 820
RruI TCGCGA 1 cut(s) 326
RsaI GTAC 2 cut(s) 1094, 1225
RsaNI GTAC 2 cut(s) 1093, 1224
SacI GAGCTC 2 cut(s) 443, 668
SalI GTCGAC 1 cut(s) 256
SaqAI TTAA 2 cut(s) 39, 884
SatI GCNGC 8 cut(s) 139, 289, 818, 821, 872, 875, 1031, 1158
Sau3AI GATC 7 cut(s) 22, 28, 125, 480, 640, 775, 1183
Sau96I GGNCC 1 cut(s) 700
SchI GAGTC 3 cut(s) 253, 365, 614
ScrFI CCNGG 2 cut(s) 595, 698
SduI GDGCHC 4 cut(s) 224, 443, 668, 716
SetI ASST 8 cut(s) 324, 369, 443, 668, 795, 822, 907, 1110
SfaNI GCATC 3 cut(s) 102, 275, 1160
Sfr274I CTCGAG 1 cut(s) 782
SinI GGWCC 1 cut(s) 700
SlaI CTCGAG 1 cut(s) 782
SmlI CTYRAG 1 cut(s) 782
SmoI CTYRAG 1 cut(s) 782
Sse9I AATT 7 cut(s) 119, 448, 577, 619, 719, 830, 856
SstI GAGCTC 2 cut(s) 443, 668
StyD4I CCNGG 2 cut(s) 593, 696
TaaI ACNGT 3 cut(s) 219, 1214, 1247
TaiI ACGT 1 cut(s) 369
TaqI TCGA 8 cut(s) 257, 377, 608, 656, 662, 783, 948, 1014
TaqII GACCGA 1 cut(s) 328
TasI AATT 7 cut(s) 119, 448, 577, 619, 719, 830, 856
TatI WGTACW 2 cut(s) 1092, 1223
TauI GCSGC 2 cut(s) 141, 291
TfiI GAWTC 3 cut(s) 204, 413, 836
Tru1I TTAA 2 cut(s) 39, 884
Tru9I TTAA 2 cut(s) 39, 884
TscAI CASTG 1 cut(s) 224
TseFI GTSAC 3 cut(s) 367, 484, 992
TseI GCWGC 6 cut(s) 817, 820, 871, 874, 1030, 1157
Tsp45I GTSAC 3 cut(s) 367, 484, 992
TspDTI ATGAA 7 cut(s) 129, 513, 632, 771, 840, 1100, 1179
TspGWI ACGGA 7 cut(s) 56, 216, 292, 347, 692, 1014, 1078
TspRI CASTG 1 cut(s) 224
VpaK11BI GGWCC 1 cut(s) 700
XagI CCTNNNNNAGG 1 cut(s) 191
XapI RAATTY 3 cut(s) 577, 619, 719
XhoI CTCGAG 1 cut(s) 782
XmiI GTMKAC 1 cut(s) 257
Zsp2I ATGCAT 1 cut(s) 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.