Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0001400

Physical Location & Seq

Genomic Coordinates Forward (+)

9301705 .. 9306007

Gene Structure 4303 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1106500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 936 bp

ATGGGCTGTCGAGGGGCAATCTCTCTCTCCAACACGTTTCGAATGGCACACCTTTGTATTTCCTCTCTCTCTCCTCCTATTTCCTACTCACCTTCTTGTCCGCCATTTCACCGGCGAATCTTCGTCGTTGCTTCCAGGATCCACGCAGCCTCCGCAACTTCCACCTCCATTTCACACTCGCAGCCGAAGGTCGTCGTCACCAGAGAGCGCGGCAAGAACGGCAAGCTCATCGCCTCCCTGGTCAAGCAGGGAATCAGCTGTTTGGAGCTTCCGCTCATTGAGCATACACGGGGGCCAGATCTGGATAGGCTTTCTACAGTATTAAGTGATACTACATTTGATTGGATTGTCATAACATCCCCTGAAGCGGGTTCAGTCTTCTTAGAGGCCTGGAAGGCTGCTGGAACTCCAAATGTTACGGTAGGGGTCGTGGGGGCTGGGACAGCAAGCATATTTGAGGAAGTAGTACAGTCATCGAAGCGATCCCTCAACATTGCTTTTGTGCCATCCAAAGCAACCGGCAAGGTCTTGGCTTCAGAGCTTCCTAAGAATGGGAATGAAAAATGCACCGTTTTATATCCTGCTTCTGCAAAGGCCAGCAATGAGATTGAGGAAGGTCTGTCCAATCGTGGGTTTGAGGTCACAAGGCTAAATACATACTCAACGGGACCTGTTCATCATGTCGACCAAACAGTTTTAAAGCAGGCACTCTCCGCCCCTGTCATAGCAGTTGCTTCTCCATCAGCTATTCGTGCCTGGGTCGGTCTTATTCCAGAGTCAGAGCAATGGAGCAATTCAGTTGCCTGTATTGGTGAGACAACTGCTAAGGCTGCTAAGAAATTAGGCCTCACAAATGTTTACTACCCAGCAAACCCAGGTCTTGAAGGGTGGGTGGGTAGCATACTTGAAGCGTTGCAAGCAAATGTATGTTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004852 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006725 GO:0006778 GO:0006779 GO:0006780 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016829 GO:0016835 GO:0016836 GO:0018130 GO:0019438 GO:0019752 GO:0033013 GO:0033014 GO:0034641 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046483 GO:0046502 GO:0051186 GO:0051188 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00860 ko01100 ko01110 ko01120 map00860 map01100 map01110 map01120

Pfam Domains

Protein Families

HEM4

Protein Analysis

312

Amino Acids

32.89

Weight (kDa)

8.82

Isoelectric Point (pI)

43.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HEM4	PF02602	76 - 298	4.2e-45	Uroporphyrinogen-III synthase HemD

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001400)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26540 AT2G26540 AT2G26540
fragaria_vesca	FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880 FvH4_2g34880
malus_domestica	MD08G1106500.v1.1 MD15G1087100.v1.1
prunus_persica	Prupe.1G440800_v2.0.a1 Prupe.1G440800_v2.0.a1 Prupe.1G440800_v2.0.a1 Prupe.1G440800_v2.0.a1 Prupe.1G440800_v2.0.a1
pyrus_communis	pycom08g08790 pycom15g08200
rosa_chinensis	RchiOBHm_Chr6g0307161
rosa_laevigata	RLG00000004395 RLG00000010732
rosa_multiflora	Rmu_sc0001776.1_g000009
rosa_roxburghii	Rroxscaffold_1G00011040 Rroxscaffold_2G00117190 Rroxscaffold_7G00161130
rosa_rugosa	Rorug06G0356900 Rorug06G0357000 Rorug06G0357000
rosa_samantha	Rh1BG065500 Rh6AG227000 Rh6AG467900 Rh6BG432600 Rh6CG483100 Rh6DG469600
rosa_wichuraiana	Rw6G040770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	274
AccI	GTMKAC	1 cut(s)	684
AccII	CGCG	1 cut(s)	210
AciI	CCGC	6 cut(s)	101, 153, 210, 272, 368, 714
AclWI	GGATC	3 cut(s)	133, 146, 477
AcuI	CTGAAG	2 cut(s)	384, 519
AfaI	GTAC	1 cut(s)	468
AfiI	CCNNNNNNNGG	4 cut(s)	367, 368, 551, 630
AflIII	ACRYGT	1 cut(s)	33
AgsI	TTSAA	2 cut(s)	884, 908
AjnI	CCWGG	5 cut(s)	134, 237, 389, 755, 874
AluBI	AGCT	5 cut(s)	226, 258, 268, 541, 746
AluI	AGCT	5 cut(s)	226, 258, 268, 541, 746
Alw26I	GTCTC	1 cut(s)	809
AlwI	GGATC	3 cut(s)	133, 146, 477
AoxI	GGCC	4 cut(s)	293, 387, 594, 844
ApeKI	GCWGC	4 cut(s)	146, 181, 398, 830
AspLEI	GCGC	1 cut(s)	210
AspS9I	GGNCC	2 cut(s)	293, 668
AsuHPI	GGTGA	4 cut(s)	81, 101, 190, 824
AsuII	TTCGAA	1 cut(s)	40
AvaII	GGWCC	1 cut(s)	668
BamHI	GGATCC	1 cut(s)	138
BbsI	GAAGAC	1 cut(s)	370
BbvI	GCAGC	4 cut(s)	158, 193, 385, 817
BccI	CCATC	2 cut(s)	514, 748
BceAI	ACGGC	1 cut(s)	235
BcgI	CGANNNNNNTGC	2 cut(s)	211, 245
BciT130I	CCWGG	5 cut(s)	136, 239, 391, 757, 876
BcoDI	GTCTC	1 cut(s)	809
BfmI	CTRYAG	1 cut(s)	315
BglI	GCCNNNNNGGC	1 cut(s)	395
BglII	AGATCT	1 cut(s)	298
BisI	GCNGC	5 cut(s)	147, 182, 211, 399, 831
BlsI	GCNGC	5 cut(s)	148, 183, 212, 400, 832
Bme1390I	CCNGG	5 cut(s)	136, 239, 391, 757, 876
Bme18I	GGWCC	1 cut(s)	668
BmgT120I	GGNCC	2 cut(s)	293, 668
BmiI	GGNNCC	3 cut(s)	140, 294, 669
BmrFI	CCNGG	5 cut(s)	136, 239, 391, 757, 876
BpiI	GAAGAC	1 cut(s)	370
Bpu10I	CCTNAGC	1 cut(s)	825
Bpu14I	TTCGAA	1 cut(s)	40
BsaJI	CCNNGG	3 cut(s)	237, 756, 874
BsaXI	ACNNNNNCTCC	2 cut(s)	134, 164
Bsc4I	CCNNNNNNNGG	4 cut(s)	367, 368, 551, 630
Bse118I	RCCGGY	2 cut(s)	111, 518
Bse3DI	GCAATG	3 cut(s)	492, 607, 791
BseBI	CCWGG	5 cut(s)	136, 239, 391, 757, 876
BseDI	CCNNGG	3 cut(s)	237, 756, 874
BseGI	GGATG	2 cut(s)	356, 506
BseLI	CCNNNNNNNGG	4 cut(s)	367, 368, 551, 630
BseMI	GCAATG	3 cut(s)	492, 607, 791
BseRI	GAGGAG	1 cut(s)	63
BseXI	GCAGC	4 cut(s)	158, 193, 385, 817
BseYI	CCCAGC	2 cut(s)	437, 865
Bsh1236I	CGCG	1 cut(s)	210
BshFI	GGCC	4 cut(s)	295, 389, 596, 846
BsiSI	CCGG	2 cut(s)	112, 519
BslFI	GGGAC	2 cut(s)	454, 681
BslI	CCNNNNNNNGG	4 cut(s)	367, 368, 551, 630
BsmAI	GTCTC	1 cut(s)	809
BsmFI	GGGAC	2 cut(s)	454, 681
BsnI	GGCC	4 cut(s)	295, 389, 596, 846
Bsp119I	TTCGAA	1 cut(s)	40
Bsp143I	GATC	3 cut(s)	138, 298, 482
BspACI	CCGC	6 cut(s)	101, 153, 210, 272, 368, 714
BspANI	GGCC	4 cut(s)	295, 389, 596, 846
BspFNI	CGCG	1 cut(s)	210
BspLI	GGNNCC	3 cut(s)	140, 294, 669
BspPI	GGATC	3 cut(s)	133, 146, 477
BspT104I	TTCGAA	1 cut(s)	40
BsrBI	CCGCTC	1 cut(s)	274
BsrDI	GCAATG	3 cut(s)	492, 607, 791
BsrFI	RCCGGY	2 cut(s)	111, 518
BssAI	RCCGGY	2 cut(s)	111, 518
BssECI	CCNNGG	3 cut(s)	237, 756, 874
BssMI	GATC	3 cut(s)	138, 298, 482
Bst2UI	CCWGG	5 cut(s)	136, 239, 391, 757, 876
Bst4CI	ACNGT	5 cut(s)	319, 421, 471, 571, 694
BstBI	TTCGAA	1 cut(s)	40
BstC8I	GCNNGC	5 cut(s)	224, 448, 598, 705, 918
BstDEI	CTNAG	4 cut(s)	382, 546, 825, 834
BstF5I	GGATG	2 cut(s)	356, 506
BstFNI	CGCG	1 cut(s)	210
BstHHI	GCGC	1 cut(s)	210
BstKTI	GATC	3 cut(s)	141, 301, 485
BstMAI	GTCTC	1 cut(s)	809
BstMBI	GATC	3 cut(s)	138, 298, 482
BstMWI	GCNNNNNNNGC	9 cut(s)	152, 219, 280, 395, 443, 713, 752, 830, 917
BstNI	CCWGG	5 cut(s)	136, 239, 391, 757, 876
BstSCI	CCNGG	5 cut(s)	134, 237, 389, 755, 874
BstSFI	CTRYAG	1 cut(s)	315
BstUI	CGCG	1 cut(s)	210
BstV1I	GCAGC	4 cut(s)	158, 193, 385, 817
BstV2I	GAAGAC	1 cut(s)	370
BstX2I	RGATCY	2 cut(s)	138, 298
BstYI	RGATCY	2 cut(s)	138, 298
BsuRI	GGCC	4 cut(s)	295, 389, 596, 846
BtgZI	GCGATG	1 cut(s)	214
BtsCI	GGATG	2 cut(s)	356, 506
Cac8I	GCNNGC	5 cut(s)	224, 448, 598, 705, 918
CfoI	GCGC	1 cut(s)	210
Cfr10I	RCCGGY	2 cut(s)	111, 518
Cfr13I	GGNCC	2 cut(s)	293, 668
Csp6I	GTAC	1 cut(s)	467
CviAII	CATG	1 cut(s)	680
CviQI	GTAC	1 cut(s)	467
DdeI	CTNAG	4 cut(s)	382, 546, 825, 834
DpnI	GATC	3 cut(s)	140, 300, 484
DpnII	GATC	3 cut(s)	138, 298, 482
DraI	TTTAAA	1 cut(s)	699
EciI	GGCGGA	2 cut(s)	90, 703
Eco147I	AGGCCT	2 cut(s)	389, 846
Eco47I	GGWCC	1 cut(s)	668
Eco57I	CTGAAG	2 cut(s)	384, 519
EcoO109I	RGGNCCY	1 cut(s)	668
EcoRII	CCWGG	5 cut(s)	134, 237, 389, 755, 874
FaeI	CATG	1 cut(s)	683
FaqI	GGGAC	2 cut(s)	454, 681
FatI	CATG	1 cut(s)	679
FauI	CCCGC	1 cut(s)	361
FblI	GTMKAC	1 cut(s)	684
Fnu4HI	GCNGC	5 cut(s)	147, 182, 211, 399, 831
FokI	GGATG	2 cut(s)	343, 493
Fsp4HI	GCNGC	5 cut(s)	147, 182, 211, 399, 831
GlaI	GCGC	1 cut(s)	209
GluI	GCNGC	5 cut(s)	147, 182, 211, 399, 831
GsaI	CCCAGC	2 cut(s)	441, 869
HaeIII	GGCC	4 cut(s)	295, 389, 596, 846
HapII	CCGG	2 cut(s)	112, 519
HhaI	GCGC	1 cut(s)	210
Hin1II	CATG	1 cut(s)	683
Hin6I	GCGC	1 cut(s)	208
HinP1I	GCGC	1 cut(s)	208
HincII	GTYRAC	1 cut(s)	685
HindII	GTYRAC	1 cut(s)	685
HinfI	GANTC	3 cut(s)	117, 252, 776
HpaII	CCGG	2 cut(s)	112, 519
HphI	GGTGA	4 cut(s)	81, 101, 190, 824
Hpy166II	GTNNAC	2 cut(s)	685, 859
Hpy188I	TCNGA	2 cut(s)	538, 781
Hpy188III	TCNNGA	3 cut(s)	302, 773, 881
Hpy8I	GTNNAC	2 cut(s)	685, 859
Hpy99I	CGWCG	2 cut(s)	128, 197
HpyAV	CCTTC	5 cut(s)	102, 181, 388, 608, 878
HpyCH4III	ACNGT	5 cut(s)	319, 421, 471, 571, 694
HpyCH4IV	ACGT	1 cut(s)	35
HpyCH4V	TGCA	3 cut(s)	567, 590, 916
HpyF10VI	GCNNNNNNNGC	9 cut(s)	152, 219, 280, 395, 443, 713, 752, 830, 917
HpyF3I	CTNAG	4 cut(s)	382, 546, 825, 834
HpySE526I	ACGT	1 cut(s)	35
Hsp92II	CATG	1 cut(s)	683
HspAI	GCGC	1 cut(s)	208
Kzo9I	GATC	3 cut(s)	138, 298, 482
LmnI	GCTCC	2 cut(s)	265, 789
Lsp1109I	GCAGC	4 cut(s)	158, 193, 385, 817
MaeII	ACGT	1 cut(s)	35
MaeIII	GTNAC	3 cut(s)	196, 415, 640
MalI	GATC	3 cut(s)	140, 300, 484
MbiI	CCGCTC	1 cut(s)	274
MboI	GATC	3 cut(s)	138, 298, 482
MboII	GAAGA	2 cut(s)	112, 370
MflI	RGATCY	2 cut(s)	138, 298
MluCI	AATT	2 cut(s)	793, 839
MlyI	GAGTC	1 cut(s)	785
MmeI	TCCRAC	1 cut(s)	54
MseI	TTAA	2 cut(s)	323, 698
MspA1I	CMGCKG	1 cut(s)	258
MspI	CCGG	2 cut(s)	112, 519
MspR9I	CCNGG	5 cut(s)	136, 239, 391, 757, 876
MvaI	CCWGG	5 cut(s)	136, 239, 391, 757, 876
MvnI	CGCG	1 cut(s)	210
MwoI	GCNNNNNNNGC	9 cut(s)	152, 219, 280, 395, 443, 713, 752, 830, 917
NdeII	GATC	3 cut(s)	138, 298, 482
NlaIII	CATG	1 cut(s)	683
NlaIV	GGNNCC	3 cut(s)	140, 294, 669
NmuCI	GTSAC	2 cut(s)	196, 640
NspV	TTCGAA	1 cut(s)	40
PceI	AGGCCT	2 cut(s)	389, 846
PfeI	GAWTC	2 cut(s)	117, 252
PfoI	TCCNGGA	1 cut(s)	134
PkrI	GCNGC	5 cut(s)	148, 183, 212, 400, 832
PleI	GAGTC	1 cut(s)	784
PpsI	GAGTC	1 cut(s)	784
PpuMI	RGGWCCY	1 cut(s)	668
Psp5II	RGGWCCY	1 cut(s)	668
Psp6I	CCWGG	5 cut(s)	134, 237, 389, 755, 874
PspFI	CCCAGC	2 cut(s)	437, 865
PspGI	CCWGG	5 cut(s)	134, 237, 389, 755, 874
PspN4I	GGNNCC	3 cut(s)	140, 294, 669
PspPI	GGNCC	2 cut(s)	293, 668
PspPPI	RGGWCCY	1 cut(s)	668
PsuI	RGATCY	2 cut(s)	138, 298
PvuII	CAGCTG	1 cut(s)	258
RsaI	GTAC	1 cut(s)	468
RsaNI	GTAC	1 cut(s)	467
SalI	GTCGAC	1 cut(s)	683
SaqAI	TTAA	2 cut(s)	323, 698
SatI	GCNGC	5 cut(s)	147, 182, 211, 399, 831
Sau3AI	GATC	3 cut(s)	138, 298, 482
Sau96I	GGNCC	2 cut(s)	293, 668
SchI	GAGTC	1 cut(s)	785
ScrFI	CCNGG	5 cut(s)	136, 239, 391, 757, 876
SfcI	CTRYAG	1 cut(s)	315
SfuI	TTCGAA	1 cut(s)	40
SgrAI	CRCCGGYG	1 cut(s)	111
SinI	GGWCC	1 cut(s)	668
Sse9I	AATT	2 cut(s)	793, 839
SseBI	AGGCCT	2 cut(s)	389, 846
SsiI	CCGC	6 cut(s)	101, 153, 210, 272, 368, 714
StuI	AGGCCT	2 cut(s)	389, 846
StyD4I	CCNGG	5 cut(s)	134, 237, 389, 755, 874
TaaI	ACNGT	5 cut(s)	319, 421, 471, 571, 694
TaiI	ACGT	1 cut(s)	38
TaqI	TCGA	4 cut(s)	10, 40, 476, 684
TaqII	GACCGA	1 cut(s)	752
TasI	AATT	2 cut(s)	793, 839
TatI	WGTACW	1 cut(s)	466
TauI	GCSGC	1 cut(s)	213
TfiI	GAWTC	2 cut(s)	117, 252
Tru1I	TTAA	2 cut(s)	323, 698
Tru9I	TTAA	2 cut(s)	323, 698
TseFI	GTSAC	2 cut(s)	196, 640
TseI	GCWGC	4 cut(s)	146, 181, 398, 830
Tsp45I	GTSAC	2 cut(s)	196, 640
TspDTI	ATGAA	3 cut(s)	573, 665, 921
VpaK11BI	GGWCC	1 cut(s)	668
XmiI	GTMKAC	1 cut(s)	684

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.