MD08G1127700.v1.1

RNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010172
Physical Location & Seq
Forward (+)
11972198 .. 11973665
1468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1127700.v1.1.491

Sequence Viewer

Length: 732 bp
ATGGACGTCCTGGTGGGCCCGACCTTCTCCATCGACGTGTCTTCCTACGGCCCTGCGCAGACGCAGGACAACCGCCACCGCAGTGGCCTGTACTTGAATCAGAACCGAGGCGCCGCGGTAGTGGAAGAGGCCTCGTCGGACAGCTCGTCATCGATCGGAGTTCCGGATGACAGCGAGGAAGAGGAAGATTCCAAAGGTGATGACGGCGATGAGGTGCAGAGCAAGTTTAACAGTGGCGGAGGAAGTGGACGAGGATTAGGTTCTCTGGGTTCATTCGGATCACTGGAAGAGTCTCTTCCAATCAAGAGGGGATTATCCAACTATTTTTCAGGAAAATCAAAGTCATTTGCCAGCCTGTCAGAAGTGTGTAGCTCTGTGAGTTCAGTGAAGGAGGTAGAGAAGCGAGACAACCCGTTCAACAAGCGGCGGCGGGTTCTGATCGCCTCCAAGTGGTCGAGAAAATCTTCCTCGTCCTCCTCCCTCTACAACTGGCCAAACCCTAAATCCATGCCGCTGCTAGCCCTAGCCGAAGAAGGAGACGAAGACGATGACGAAGAACAGAACCGTGACCGGCAAGGCGACGGAGACAACGCGTCGCAGCAATCCTCGTCGGACGAGGAGGATCAGGAGAGGAGGAGGGCCCCACAGAAACTGCTACACAGGAAGCTGAAGAGCTTCAAGTCCAAGAGTTGCTATTGCCTCTCAGATCTGCAGGAACATGATGAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

244

Amino Acids

26.66

Weight (kDa)

4.86

Isoelectric Point (pI)

71.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 9
Acc16I TGCGCA 1 cut(s) 57
AccB1I GGYRCC 1 cut(s) 110
AccII CGCG 2 cut(s) 116, 593
AccIII TCCGGA 1 cut(s) 163
AciI CCGC 9 cut(s) 73, 79, 114, 116, 237, 424, 427, 430, 512
AclWI GGATC 2 cut(s) 286, 630
AcoI YGGCCR 1 cut(s) 491
AcuI CTGAAG 1 cut(s) 689
AcyI GRCGYC 2 cut(s) 6, 111
AfaI GTAC 1 cut(s) 92
AfiI CCNNNNNNNGG 1 cut(s) 450
AflIII ACRYGT 2 cut(s) 36, 591
AgsI TTSAA 3 cut(s) 97, 418, 679
AhdI GACNNNNNGTC 2 cut(s) 145, 592
AjiI CACGTC 1 cut(s) 37
AjnI CCWGG 1 cut(s) 9
AleI CACNNNNGTG 1 cut(s) 81
AluBI AGCT 4 cut(s) 144, 372, 667, 675
AluI AGCT 4 cut(s) 144, 372, 667, 675
Alw26I GTCTC 4 cut(s) 297, 399, 531, 579
AlwI GGATC 2 cut(s) 286, 630
AlwNI CAGNNNCTG 1 cut(s) 652
Aor13HI TCCGGA 1 cut(s) 163
AoxI GGCC 6 cut(s) 16, 49, 85, 129, 491, 639
ApaI GGGCCC 2 cut(s) 20, 643
ApeKI GCWGC 2 cut(s) 514, 598
Asp700I GAANNNNTTC 2 cut(s) 463, 674
AspLEI GCGC 2 cut(s) 58, 113
AspS9I GGNCC 5 cut(s) 16, 17, 50, 639, 640
AsuHPI GGTGA 1 cut(s) 209
AsuNHI GCTAGC 1 cut(s) 517
BaeGI GKGCMC 2 cut(s) 20, 643
BalI TGGCCA 1 cut(s) 493
BanI GGYRCC 1 cut(s) 110
BanII GRGCYC 2 cut(s) 20, 643
BbsI GAAGAC 2 cut(s) 33, 549
BbvI GCAGC 2 cut(s) 501, 610
BccI CCATC 1 cut(s) 38
BceAI ACGGC 2 cut(s) 64, 220
BciT130I CCWGG 1 cut(s) 11
BcoDI GTCTC 4 cut(s) 297, 399, 531, 579
BfaI CTAG 2 cut(s) 518, 524
BfmI CTRYAG 1 cut(s) 710
BfoI RGCGCY 1 cut(s) 114
BglII AGATCT 1 cut(s) 706
BisI GCNGC 6 cut(s) 114, 425, 428, 512, 515, 599
BlsI GCNGC 6 cut(s) 115, 426, 429, 513, 516, 600
Bme1390I CCNGG 1 cut(s) 11
BmeRI GACNNNNNGTC 2 cut(s) 145, 592
BmgBI CACGTC 1 cut(s) 37
BmgT120I GGNCC 5 cut(s) 16, 17, 50, 639, 640
BmiI GGNNCC 4 cut(s) 18, 112, 641, 642
BmrFI CCNGG 1 cut(s) 11
BmtI GCTAGC 1 cut(s) 521
BpiI GAAGAC 2 cut(s) 33, 549
Bsa29I ATCGAT 1 cut(s) 152
BsaHI GRCGYC 2 cut(s) 6, 111
BsaJI CCNNGG 2 cut(s) 106, 114
BsaWI WCCGGW 1 cut(s) 163
Bsc4I CCNNNNNNNGG 1 cut(s) 450
Bse118I RCCGGY 1 cut(s) 570
Bse1I ACTGG 2 cut(s) 288, 494
BseAI TCCGGA 1 cut(s) 163
BseBI CCWGG 1 cut(s) 11
BseCI ATCGAT 1 cut(s) 152
BseDI CCNNGG 2 cut(s) 106, 114
BseGI GGATG 1 cut(s) 172
BseLI CCNNNNNNNGG 1 cut(s) 450
BseMII CTCAG 1 cut(s) 717
BseNI ACTGG 2 cut(s) 288, 494
BseRI GAGGAG 4 cut(s) 466, 632, 646, 649
BseSI GKGCMC 2 cut(s) 20, 643
BseXI GCAGC 2 cut(s) 501, 610
BsgI GTGCAG 1 cut(s) 236
Bsh1236I CGCG 2 cut(s) 116, 593
Bsh1285I CGRYCG 1 cut(s) 156
BshFI GGCC 6 cut(s) 18, 51, 87, 131, 493, 641
BshNI GGYRCC 1 cut(s) 110
BshVI ATCGAT 1 cut(s) 152
BsiEI CGRYCG 1 cut(s) 156
BsiSI CCGG 2 cut(s) 164, 571
BslI CCNNNNNNNGG 1 cut(s) 450
BsmAI GTCTC 4 cut(s) 297, 399, 531, 579
BsmBI CGTCTC 1 cut(s) 531
BsnI GGCC 6 cut(s) 18, 51, 87, 131, 493, 641
Bsp120I GGGCCC 2 cut(s) 16, 639
Bsp1286I GDGCHC 2 cut(s) 20, 643
Bsp13I TCCGGA 1 cut(s) 163
Bsp143I GATC 5 cut(s) 153, 278, 438, 622, 706
BspACI CCGC 9 cut(s) 73, 79, 114, 116, 237, 424, 427, 430, 512
BspANI GGCC 6 cut(s) 18, 51, 87, 131, 493, 641
BspCNI CTCAG 1 cut(s) 716
BspDI ATCGAT 1 cut(s) 152
BspEI TCCGGA 1 cut(s) 163
BspFNI CGCG 2 cut(s) 116, 593
BspLI GGNNCC 4 cut(s) 18, 112, 641, 642
BspMAI CTGCAG 1 cut(s) 714
BspOI GCTAGC 1 cut(s) 521
BspPI GGATC 2 cut(s) 286, 630
BspQI GCTCTTC 1 cut(s) 665
BspT107I GGYRCC 1 cut(s) 110
BsrFI RCCGGY 1 cut(s) 570
BsrI ACTGG 2 cut(s) 288, 494
BssAI RCCGGY 1 cut(s) 570
BssECI CCNNGG 2 cut(s) 106, 114
BssMI GATC 5 cut(s) 153, 278, 438, 622, 706
BssNI GRCGYC 2 cut(s) 6, 111
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 3 cut(s) 233, 566, 729
Bst6I CTCTTC 5 cut(s) 120, 174, 282, 300, 665
BstACI GRCGYC 2 cut(s) 6, 111
BstC8I GCNNGC 2 cut(s) 352, 519
BstDEI CTNAG 1 cut(s) 703
BstDSI CCRYGG 1 cut(s) 114
BstF5I GGATG 1 cut(s) 172
BstFNI CGCG 2 cut(s) 116, 593
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 2 cut(s) 58, 113
BstKTI GATC 5 cut(s) 156, 281, 441, 625, 709
BstMAI GTCTC 4 cut(s) 297, 399, 531, 579
BstMBI GATC 5 cut(s) 153, 278, 438, 622, 706
BstMCI CGRYCG 1 cut(s) 156
BstNI CCWGG 1 cut(s) 11
BstSCI CCNGG 1 cut(s) 9
BstSFI CTRYAG 1 cut(s) 710
BstSLI GKGCMC 2 cut(s) 20, 643
BstUI CGCG 2 cut(s) 116, 593
BstV1I GCAGC 2 cut(s) 501, 610
BstV2I GAAGAC 2 cut(s) 33, 549
BstX2I RGATCY 1 cut(s) 706
BstXI CCANNNNNNTGG 1 cut(s) 83
BstYI RGATCY 1 cut(s) 706
Bsu15I ATCGAT 1 cut(s) 152
BsuRI GGCC 6 cut(s) 18, 51, 87, 131, 493, 641
BsuTUI ATCGAT 1 cut(s) 152
BtgI CCRYGG 1 cut(s) 114
BtgZI GCGATG 1 cut(s) 222
BtrI CACGTC 1 cut(s) 37
BtsCI GGATG 1 cut(s) 172
BtsI GCAGTG 1 cut(s) 88
BtsIMutI CAGTG 4 cut(s) 88, 238, 281, 390
Cac8I GCNNGC 2 cut(s) 352, 519
CaiI CAGNNNCTG 1 cut(s) 652
CfoI GCGC 2 cut(s) 58, 113
Cfr10I RCCGGY 1 cut(s) 570
Cfr13I GGNCC 5 cut(s) 16, 17, 50, 639, 640
Cfr42I CCGCGG 1 cut(s) 117
ClaI ATCGAT 1 cut(s) 152
CseI GACGC 2 cut(s) 70, 582
Csp6I GTAC 1 cut(s) 91
CviAII CATG 2 cut(s) 508, 719
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 1 cut(s) 703
DinI GGCGCC 1 cut(s) 112
DpnI GATC 5 cut(s) 155, 280, 440, 624, 708
DpnII GATC 5 cut(s) 153, 278, 438, 622, 706
DriI GACNNNNNGTC 2 cut(s) 145, 592
EaeI YGGCCR 1 cut(s) 491
Eam1104I CTCTTC 5 cut(s) 120, 174, 282, 300, 665
Eam1105I GACNNNNNGTC 2 cut(s) 145, 592
EarI CTCTTC 5 cut(s) 120, 174, 282, 300, 665
EciI GGCGGA 1 cut(s) 252
Eco147I AGGCCT 1 cut(s) 131
Eco24I GRGCYC 2 cut(s) 20, 643
Eco57I CTGAAG 1 cut(s) 689
EcoO109I RGGNCCY 2 cut(s) 639, 640
EcoRII CCWGG 1 cut(s) 9
EcoT38I GRGCYC 2 cut(s) 20, 643
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
Esp3I CGTCTC 1 cut(s) 531
FaeI CATG 2 cut(s) 511, 722
FaiI YATR 2 cut(s) 509, 720
FalI AAGNNNNNCTT 2 cut(s) 279, 311
FatI CATG 2 cut(s) 507, 718
FauI CCCGC 1 cut(s) 423
Fnu4HI GCNGC 6 cut(s) 114, 425, 428, 512, 515, 599
FokI GGATG 1 cut(s) 179
FriOI GRGCYC 2 cut(s) 20, 643
Fsp4HI GCNGC 6 cut(s) 114, 425, 428, 512, 515, 599
FspBI CTAG 2 cut(s) 518, 524
FspI TGCGCA 1 cut(s) 57
GlaI GCGC 2 cut(s) 57, 112
GluI GCNGC 6 cut(s) 114, 425, 428, 512, 515, 599
HaeII RGCGCY 1 cut(s) 114
HaeIII GGCC 6 cut(s) 18, 51, 87, 131, 493, 641
HapII CCGG 2 cut(s) 164, 571
HgaI GACGC 2 cut(s) 70, 582
HhaI GCGC 2 cut(s) 58, 113
Hin1I GRCGYC 2 cut(s) 6, 111
Hin1II CATG 2 cut(s) 511, 722
Hin6I GCGC 2 cut(s) 56, 111
HinP1I GCGC 2 cut(s) 56, 111
HinfI GANTC 3 cut(s) 97, 188, 290
HpaII CCGG 2 cut(s) 164, 571
HphI GGTGA 1 cut(s) 209
Hpy166II GTNNAC 1 cut(s) 248
Hpy188I TCNGA 8 cut(s) 102, 139, 158, 278, 361, 438, 613, 706
Hpy188III TCNNGA 5 cut(s) 164, 304, 330, 456, 626
Hpy8I GTNNAC 1 cut(s) 248
Hpy99I CGWCG 5 cut(s) 38, 139, 584, 598, 613
HpyAV CCTTC 3 cut(s) 34, 382, 527
HpyCH4III ACNGT 3 cut(s) 233, 566, 729
HpyCH4IV ACGT 2 cut(s) 6, 36
HpyCH4V TGCA 2 cut(s) 217, 712
HpyF3I CTNAG 1 cut(s) 703
HpySE526I ACGT 2 cut(s) 6, 36
Hsp92I GRCGYC 2 cut(s) 6, 111
Hsp92II CATG 2 cut(s) 511, 722
HspAI GCGC 2 cut(s) 56, 111
KasI GGCGCC 1 cut(s) 110
Kpn2I TCCGGA 1 cut(s) 163
KspI CCGCGG 1 cut(s) 117
Kzo9I GATC 5 cut(s) 153, 278, 438, 622, 706
LguI GCTCTTC 1 cut(s) 665
Lsp1109I GCAGC 2 cut(s) 501, 610
MaeI CTAG 2 cut(s) 518, 524
MaeII ACGT 2 cut(s) 6, 36
MaeIII GTNAC 1 cut(s) 566
MalI GATC 5 cut(s) 155, 280, 440, 624, 708
MboI GATC 5 cut(s) 153, 278, 438, 622, 706
MflI RGATCY 1 cut(s) 706
MhlI GDGCHC 2 cut(s) 20, 643
MlsI TGGCCA 1 cut(s) 493
MluI ACGCGT 1 cut(s) 591
MluNI TGGCCA 1 cut(s) 493
Mly113I GGCGCC 1 cut(s) 111
MlyI GAGTC 1 cut(s) 299
MmeI TCCRAC 3 cut(s) 117, 342, 591
Mox20I TGGCCA 1 cut(s) 493
MroI TCCGGA 1 cut(s) 163
MroXI GAANNNNTTC 2 cut(s) 463, 674
MscI TGGCCA 1 cut(s) 493
MseI TTAA 1 cut(s) 228
MslI CAYNNNNRTG 2 cut(s) 35, 81
Msp20I TGGCCA 1 cut(s) 493
MspA1I CMGCKG 2 cut(s) 116, 514
MspI CCGG 2 cut(s) 164, 571
MspR9I CCNGG 1 cut(s) 11
MvaI CCWGG 1 cut(s) 11
MvnI CGCG 2 cut(s) 116, 593
NarI GGCGCC 1 cut(s) 111
NdeII GATC 5 cut(s) 153, 278, 438, 622, 706
NheI GCTAGC 1 cut(s) 517
NlaIII CATG 2 cut(s) 511, 722
NlaIV GGNNCC 4 cut(s) 18, 112, 641, 642
NmuCI GTSAC 1 cut(s) 566
NsbI TGCGCA 1 cut(s) 57
OliI CACNNNNGTG 1 cut(s) 81
PceI AGGCCT 1 cut(s) 131
PciSI GCTCTTC 1 cut(s) 665
PcsI WCGNNNNNNNCGW 1 cut(s) 143
PdmI GAANNNNTTC 2 cut(s) 463, 674
PfeI GAWTC 2 cut(s) 97, 188
PkrI GCNGC 6 cut(s) 115, 426, 429, 513, 516, 600
Ple19I CGATCG 1 cut(s) 156
PleI GAGTC 1 cut(s) 298
PluTI GGCGCC 1 cut(s) 114
PpsI GAGTC 1 cut(s) 298
Psp6I CCWGG 1 cut(s) 9
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 4 cut(s) 18, 112, 641, 642
PspOMI GGGCCC 2 cut(s) 16, 639
PspPI GGNCC 5 cut(s) 16, 17, 50, 639, 640
PstI CTGCAG 1 cut(s) 714
PstNI CAGNNNCTG 1 cut(s) 652
PsuI RGATCY 1 cut(s) 706
PvuI CGATCG 1 cut(s) 156
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
RseI CAYNNNNRTG 2 cut(s) 35, 81
SacII CCGCGG 1 cut(s) 117
SapI GCTCTTC 1 cut(s) 665
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 6 cut(s) 114, 425, 428, 512, 515, 599
Sau3AI GATC 5 cut(s) 153, 278, 438, 622, 706
Sau96I GGNCC 5 cut(s) 16, 17, 50, 639, 640
SchI GAGTC 1 cut(s) 299
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 2 cut(s) 20, 643
SfcI CTRYAG 1 cut(s) 710
SfoI GGCGCC 1 cut(s) 112
Sfr303I CCGCGG 1 cut(s) 117
SgrBI CCGCGG 1 cut(s) 117
SmiMI CAYNNNNRTG 2 cut(s) 35, 81
SseBI AGGCCT 1 cut(s) 131
SsiI CCGC 9 cut(s) 73, 79, 114, 116, 237, 424, 427, 430, 512
SspDI GGCGCC 1 cut(s) 110
SspMI CTAG 2 cut(s) 518, 524
StuI AGGCCT 1 cut(s) 131
StyD4I CCNGG 1 cut(s) 9
TaaI ACNGT 3 cut(s) 233, 566, 729
TaiI ACGT 2 cut(s) 9, 39
TaqI TCGA 3 cut(s) 33, 152, 455
TatI WGTACW 1 cut(s) 90
TauI GCSGC 4 cut(s) 116, 427, 430, 514
TfiI GAWTC 2 cut(s) 97, 188
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 4 cut(s) 88, 238, 288, 390
TseFI GTSAC 1 cut(s) 566
TseI GCWGC 2 cut(s) 514, 598
Tsp45I GTSAC 1 cut(s) 566
TspDTI ATGAA 1 cut(s) 261
TspGWI ACGGA 1 cut(s) 597
TspRI CASTG 4 cut(s) 88, 238, 288, 390
XmnI GAANNNNTTC 2 cut(s) 463, 674
XspI CTAG 2 cut(s) 518, 524
ZraI GACGTC 1 cut(s) 7
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.