pycom08g10670

RNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0010172
Physical Location & Seq
Forward (+)
8903418 .. 8904290
873 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g10670.1

Sequence Viewer

Length: 732 bp
ATGGATGTCCTGGTGGGCCCGACCTTCTCCGTCGACGTATCGTCCTACGGCCCTGCGCAGACGCAGGACAGCCGCCACCGAGCCGGCCTGTACCTGAATCAGGACCGAGGGGCCGCGGTGGCGGAAGAGGCCTCGTCGGACAGCTCGTCATCGATCGGAGTTCCGGATGACAGCGAGGAAGAGGAAGATTTCAAAGGTGACGACGGCGATGAGGTGCAGAGCAAGTTTAATGGCGGCGGAGGAAGTGGAGGAGGATTAGGTTCTCTGGGTTCATTCGGATCACTCGAAGAGTCTCTTCCAATCAAGAGGGGATTATCCAACTATTTTTCAGGAAAATCAAAGTCATTTGCCAGCCTGTCAGAAGTGTGCAGCTCTGTGAGTTCAGTGAAGGAGGTGGAGAAGCGAGACAACCCGTTCAACAAGCGGCGGCGGGTTCTGATCGCCTCCAAGTGGTCGAGAAAATCTTCCTCTTCCTCCTCCCTCTACAACTGGCCAAACCCTAAATCCATGCCGCTGCTAGCCCTAGCCGAAGAAGGAGACGAAGACGATGACGAAGAACAGAACCGTGACCGACAAGGCGACGGAGACAACGCGTCGCAGCAATCCTCGTCGGACGGGGAGGATCAGGAGAGGAGGAGGCCCCCACATAAACTGCTAGACAGGAAGCTGAAGAGCTTCAAGTCAAAGAGTTGCTACTGCCTCTCAGATCTGCAGGAACATGATGAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

244

Amino Acids

26.45

Weight (kDa)

4.74

Isoelectric Point (pI)

69.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 57
AccI GTMKAC 1 cut(s) 33
AccII CGCG 2 cut(s) 116, 593
AccIII TCCGGA 1 cut(s) 163
AclWI GGATC 2 cut(s) 286, 630
AcoI YGGCCR 1 cut(s) 491
AcuI CTGAAG 1 cut(s) 689
AfaI GTAC 1 cut(s) 92
AfiI CCNNNNNNNGG 2 cut(s) 100, 450
AflIII ACRYGT 1 cut(s) 591
AgsI TTSAA 3 cut(s) 193, 418, 679
AhdI GACNNNNNGTC 3 cut(s) 40, 145, 592
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 4 cut(s) 144, 372, 667, 675
AluI AGCT 4 cut(s) 144, 372, 667, 675
Alw26I GTCTC 4 cut(s) 297, 399, 531, 579
AlwI GGATC 2 cut(s) 286, 630
Aor13HI TCCGGA 1 cut(s) 163
AoxI GGCC 7 cut(s) 16, 49, 85, 111, 129, 491, 638
ApaI GGGCCC 1 cut(s) 20
ApeKI GCWGC 3 cut(s) 369, 514, 598
Asp700I GAANNNNTTC 2 cut(s) 463, 674
AspLEI GCGC 1 cut(s) 58
AspS9I GGNCC 6 cut(s) 16, 17, 50, 103, 111, 639
AsuHPI GGTGA 1 cut(s) 209
AsuNHI GCTAGC 1 cut(s) 517
AvaII GGWCC 1 cut(s) 103
BaeGI GKGCMC 1 cut(s) 20
BalI TGGCCA 1 cut(s) 493
BanII GRGCYC 1 cut(s) 20
BbsI GAAGAC 1 cut(s) 549
BbvI GCAGC 3 cut(s) 381, 501, 610
BceAI ACGGC 2 cut(s) 64, 220
BciT130I CCWGG 1 cut(s) 11
BcoDI GTCTC 4 cut(s) 297, 399, 531, 579
BfaI CTAG 3 cut(s) 518, 524, 656
BfmI CTRYAG 1 cut(s) 710
BglI GCCNNNNNGGC 1 cut(s) 119
BglII AGATCT 1 cut(s) 706
BisI GCNGC 9 cut(s) 73, 114, 235, 370, 425, 428, 512, 515, 599
BlsI GCNGC 9 cut(s) 74, 115, 236, 371, 426, 429, 513, 516, 600
Bme1390I CCNGG 1 cut(s) 11
Bme18I GGWCC 1 cut(s) 103
BmeRI GACNNNNNGTC 3 cut(s) 40, 145, 592
BmgT120I GGNCC 6 cut(s) 16, 17, 50, 103, 111, 639
BmiI GGNNCC 3 cut(s) 18, 112, 641
BmrFI CCNGG 1 cut(s) 11
BmtI GCTAGC 1 cut(s) 521
BpiI GAAGAC 1 cut(s) 549
Bsa29I ATCGAT 1 cut(s) 152
BsaJI CCNNGG 2 cut(s) 106, 114
BsaWI WCCGGW 1 cut(s) 163
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 450
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 1 cut(s) 494
BseAI TCCGGA 1 cut(s) 163
BseBI CCWGG 1 cut(s) 11
BseCI ATCGAT 1 cut(s) 152
BseDI CCNNGG 2 cut(s) 106, 114
BseGI GGATG 2 cut(s) 10, 172
BseLI CCNNNNNNNGG 2 cut(s) 100, 450
BseMII CTCAG 1 cut(s) 717
BseNI ACTGG 1 cut(s) 494
BseRI GAGGAG 4 cut(s) 264, 466, 646, 649
BseSI GKGCMC 1 cut(s) 20
BseXI GCAGC 3 cut(s) 381, 501, 610
BsgI GTGCAG 2 cut(s) 236, 388
Bsh1236I CGCG 2 cut(s) 116, 593
Bsh1285I CGRYCG 1 cut(s) 156
BshFI GGCC 7 cut(s) 18, 51, 87, 113, 131, 493, 640
BshVI ATCGAT 1 cut(s) 152
BsiEI CGRYCG 1 cut(s) 156
BsiSI CCGG 2 cut(s) 84, 164
BslI CCNNNNNNNGG 2 cut(s) 100, 450
BsmAI GTCTC 4 cut(s) 297, 399, 531, 579
BsmBI CGTCTC 1 cut(s) 531
BsnI GGCC 7 cut(s) 18, 51, 87, 113, 131, 493, 640
Bsp120I GGGCCC 1 cut(s) 16
Bsp1286I GDGCHC 1 cut(s) 20
Bsp13I TCCGGA 1 cut(s) 163
Bsp143I GATC 5 cut(s) 153, 278, 438, 622, 706
BspANI GGCC 7 cut(s) 18, 51, 87, 113, 131, 493, 640
BspCNI CTCAG 1 cut(s) 716
BspDI ATCGAT 1 cut(s) 152
BspEI TCCGGA 1 cut(s) 163
BspFNI CGCG 2 cut(s) 116, 593
BspLI GGNNCC 3 cut(s) 18, 112, 641
BspMAI CTGCAG 1 cut(s) 714
BspOI GCTAGC 1 cut(s) 521
BspPI GGATC 2 cut(s) 286, 630
BspQI GCTCTTC 1 cut(s) 665
BsrFI RCCGGY 1 cut(s) 83
BsrI ACTGG 1 cut(s) 494
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 2 cut(s) 106, 114
BssMI GATC 5 cut(s) 153, 278, 438, 622, 706
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 2 cut(s) 566, 729
Bst6I CTCTTC 6 cut(s) 120, 174, 282, 300, 475, 665
BstC8I GCNNGC 3 cut(s) 85, 352, 519
BstDEI CTNAG 1 cut(s) 703
BstDSI CCRYGG 1 cut(s) 114
BstENI CCTNNNNNAGG 1 cut(s) 98
BstF5I GGATG 2 cut(s) 10, 172
BstFNI CGCG 2 cut(s) 116, 593
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 5 cut(s) 156, 281, 441, 625, 709
BstMAI GTCTC 4 cut(s) 297, 399, 531, 579
BstMBI GATC 5 cut(s) 153, 278, 438, 622, 706
BstMCI CGRYCG 1 cut(s) 156
BstMWI GCNNNNNNNGC 2 cut(s) 119, 128
BstNI CCWGG 1 cut(s) 11
BstSCI CCNGG 1 cut(s) 9
BstSFI CTRYAG 1 cut(s) 710
BstSLI GKGCMC 1 cut(s) 20
BstUI CGCG 2 cut(s) 116, 593
BstV1I GCAGC 3 cut(s) 381, 501, 610
BstV2I GAAGAC 1 cut(s) 549
BstX2I RGATCY 1 cut(s) 706
BstYI RGATCY 1 cut(s) 706
Bsu15I ATCGAT 1 cut(s) 152
BsuRI GGCC 7 cut(s) 18, 51, 87, 113, 131, 493, 640
BsuTUI ATCGAT 1 cut(s) 152
BtgI CCRYGG 1 cut(s) 114
BtgZI GCGATG 1 cut(s) 222
BtsCI GGATG 2 cut(s) 10, 172
BtsIMutI CAGTG 1 cut(s) 390
Cac8I GCNNGC 3 cut(s) 85, 352, 519
CfoI GCGC 1 cut(s) 58
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 6 cut(s) 16, 17, 50, 103, 111, 639
Cfr42I CCGCGG 1 cut(s) 117
ClaI ATCGAT 1 cut(s) 152
CseI GACGC 2 cut(s) 70, 582
Csp6I GTAC 1 cut(s) 91
CviAII CATG 2 cut(s) 508, 719
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 1 cut(s) 703
DpnI GATC 5 cut(s) 155, 280, 440, 624, 708
DpnII GATC 5 cut(s) 153, 278, 438, 622, 706
DriI GACNNNNNGTC 3 cut(s) 40, 145, 592
EaeI YGGCCR 1 cut(s) 491
Eam1104I CTCTTC 6 cut(s) 120, 174, 282, 300, 475, 665
Eam1105I GACNNNNNGTC 3 cut(s) 40, 145, 592
EarI CTCTTC 6 cut(s) 120, 174, 282, 300, 475, 665
EciI GGCGGA 2 cut(s) 137, 252
Eco147I AGGCCT 1 cut(s) 131
Eco24I GRGCYC 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 103
Eco57I CTGAAG 1 cut(s) 689
EcoNI CCTNNNNNAGG 1 cut(s) 98
EcoO109I RGGNCCY 1 cut(s) 639
EcoRII CCWGG 1 cut(s) 9
EcoT38I GRGCYC 1 cut(s) 20
Esp3I CGTCTC 1 cut(s) 531
FaeI CATG 2 cut(s) 511, 722
FaiI YATR 3 cut(s) 509, 648, 720
FalI AAGNNNNNCTT 2 cut(s) 279, 311
FatI CATG 2 cut(s) 507, 718
FauI CCCGC 1 cut(s) 423
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 9 cut(s) 73, 114, 235, 370, 425, 428, 512, 515, 599
FokI GGATG 2 cut(s) 17, 179
FriOI GRGCYC 1 cut(s) 20
Fsp4HI GCNGC 9 cut(s) 73, 114, 235, 370, 425, 428, 512, 515, 599
FspBI CTAG 3 cut(s) 518, 524, 656
FspI TGCGCA 1 cut(s) 57
GlaI GCGC 1 cut(s) 57
GluI GCNGC 9 cut(s) 73, 114, 235, 370, 425, 428, 512, 515, 599
HaeIII GGCC 7 cut(s) 18, 51, 87, 113, 131, 493, 640
HapII CCGG 2 cut(s) 84, 164
HgaI GACGC 2 cut(s) 70, 582
HhaI GCGC 1 cut(s) 58
Hin1II CATG 2 cut(s) 511, 722
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HincII GTYRAC 1 cut(s) 34
HindII GTYRAC 1 cut(s) 34
HinfI GANTC 2 cut(s) 97, 290
HpaII CCGG 2 cut(s) 84, 164
HphI GGTGA 1 cut(s) 209
Hpy166II GTNNAC 1 cut(s) 34
Hpy188I TCNGA 7 cut(s) 139, 158, 278, 361, 438, 613, 706
Hpy188III TCNNGA 6 cut(s) 101, 164, 304, 330, 456, 626
Hpy8I GTNNAC 1 cut(s) 34
Hpy99I CGWCG 7 cut(s) 35, 38, 139, 206, 584, 598, 613
HpyAV CCTTC 3 cut(s) 34, 382, 527
HpyCH4III ACNGT 2 cut(s) 566, 729
HpyCH4IV ACGT 1 cut(s) 36
HpyCH4V TGCA 3 cut(s) 217, 369, 712
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 128
HpyF3I CTNAG 1 cut(s) 703
HpySE526I ACGT 1 cut(s) 36
Hsp92II CATG 2 cut(s) 511, 722
HspAI GCGC 1 cut(s) 56
Kpn2I TCCGGA 1 cut(s) 163
KroI GCCGGC 1 cut(s) 83
KroNI GCCGGC 1 cut(s) 85
KspI CCGCGG 1 cut(s) 117
Kzo9I GATC 5 cut(s) 153, 278, 438, 622, 706
LguI GCTCTTC 1 cut(s) 665
Lsp1109I GCAGC 3 cut(s) 381, 501, 610
MaeI CTAG 3 cut(s) 518, 524, 656
MaeII ACGT 1 cut(s) 36
MaeIII GTNAC 2 cut(s) 197, 566
MalI GATC 5 cut(s) 155, 280, 440, 624, 708
MboI GATC 5 cut(s) 153, 278, 438, 622, 706
MflI RGATCY 1 cut(s) 706
MhlI GDGCHC 1 cut(s) 20
MlsI TGGCCA 1 cut(s) 493
MluI ACGCGT 1 cut(s) 591
MluNI TGGCCA 1 cut(s) 493
MlyI GAGTC 1 cut(s) 299
MmeI TCCRAC 3 cut(s) 117, 342, 591
Mox20I TGGCCA 1 cut(s) 493
MroI TCCGGA 1 cut(s) 163
MroNI GCCGGC 1 cut(s) 83
MroXI GAANNNNTTC 2 cut(s) 463, 674
MscI TGGCCA 1 cut(s) 493
MseI TTAA 1 cut(s) 228
Msp20I TGGCCA 1 cut(s) 493
MspA1I CMGCKG 2 cut(s) 116, 514
MspI CCGG 2 cut(s) 84, 164
MspR9I CCNGG 1 cut(s) 11
MvaI CCWGG 1 cut(s) 11
MvnI CGCG 2 cut(s) 116, 593
MwoI GCNNNNNNNGC 2 cut(s) 119, 128
NaeI GCCGGC 1 cut(s) 85
NdeII GATC 5 cut(s) 153, 278, 438, 622, 706
NgoMIV GCCGGC 1 cut(s) 83
NheI GCTAGC 1 cut(s) 517
NlaIII CATG 2 cut(s) 511, 722
NlaIV GGNNCC 3 cut(s) 18, 112, 641
NmuCI GTSAC 2 cut(s) 197, 566
NsbI TGCGCA 1 cut(s) 57
PceI AGGCCT 1 cut(s) 131
PciSI GCTCTTC 1 cut(s) 665
PcsI WCGNNNNNNNCGW 2 cut(s) 143, 282
PdiI GCCGGC 1 cut(s) 85
PdmI GAANNNNTTC 2 cut(s) 463, 674
PfeI GAWTC 1 cut(s) 97
PkrI GCNGC 9 cut(s) 74, 115, 236, 371, 426, 429, 513, 516, 600
Ple19I CGATCG 1 cut(s) 156
PleI GAGTC 1 cut(s) 298
PpsI GAGTC 1 cut(s) 298
Psp6I CCWGG 1 cut(s) 9
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 3 cut(s) 18, 112, 641
PspOMI GGGCCC 1 cut(s) 16
PspPI GGNCC 6 cut(s) 16, 17, 50, 103, 111, 639
PstI CTGCAG 1 cut(s) 714
PsuI RGATCY 1 cut(s) 706
PvuI CGATCG 1 cut(s) 156
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
SacII CCGCGG 1 cut(s) 117
SalI GTCGAC 1 cut(s) 32
SapI GCTCTTC 1 cut(s) 665
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 9 cut(s) 73, 114, 235, 370, 425, 428, 512, 515, 599
Sau3AI GATC 5 cut(s) 153, 278, 438, 622, 706
Sau96I GGNCC 6 cut(s) 16, 17, 50, 103, 111, 639
SchI GAGTC 1 cut(s) 299
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 1 cut(s) 20
SfcI CTRYAG 1 cut(s) 710
Sfr303I CCGCGG 1 cut(s) 117
SgrBI CCGCGG 1 cut(s) 117
SgrDI CGTCGACG 1 cut(s) 32
SinI GGWCC 1 cut(s) 103
SseBI AGGCCT 1 cut(s) 131
SspMI CTAG 3 cut(s) 518, 524, 656
StuI AGGCCT 1 cut(s) 131
StyD4I CCNGG 1 cut(s) 9
TaaI ACNGT 2 cut(s) 566, 729
TaiI ACGT 1 cut(s) 39
TaqI TCGA 4 cut(s) 33, 152, 285, 455
TaqII GACCGA 2 cut(s) 120, 585
TauI GCSGC 6 cut(s) 75, 116, 237, 427, 430, 514
TfiI GAWTC 1 cut(s) 97
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 1 cut(s) 390
TseFI GTSAC 2 cut(s) 197, 566
TseI GCWGC 3 cut(s) 369, 514, 598
Tsp45I GTSAC 2 cut(s) 197, 566
TspDTI ATGAA 1 cut(s) 261
TspGWI ACGGA 2 cut(s) 19, 597
TspRI CASTG 1 cut(s) 390
VpaK11BI GGWCC 1 cut(s) 103
XagI CCTNNNNNAGG 1 cut(s) 98
XmiI GTMKAC 1 cut(s) 33
XmnI GAANNNNTTC 2 cut(s) 463, 674
XspI CTAG 3 cut(s) 518, 524, 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.