Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0005675

Physical Location & Seq

Genomic Coordinates Forward (+)

1164310 .. 1166281

Gene Structure 1972 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1019000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1020 bp

ATGGCGAATTTGCTGATCAACAGAACCCTAAAATCAGTCGGAAACCTCAAACTGATCGGATCTCTCTCTTCTCGAGCTCGCCCCTTCAGCTCCACCGCCACCGCTACTTTCGAGCAGCCCCAATCCGACGGCCCATCTTCGTCATTCACCTTCTCCTCGGACGGAAAAGAAGACAAGAATAACAGCAGTGCCCACGATGACAGCATATACGTCAAGTCGCCGTCGAGTTCCAGCTCCAAGAGCGAAGCGGCGTCGTCGGTGACGATGCCGATGTCGTTCATGACGGGGTCCATCGTGGGAAAGAGATTTTACAAACAGGTGACGACGAGGCCGGCCGACGATGGCAATGGTTGGACTGTGATGCTTGATTACCGGACACTCAAAACGCCATCGAAGAGGCCCCTAAAGCTTCCTACTTTAGCCCTGGCGAAGGCTATTGCGGCAGAATGGGAATACCAGGAAACAGATGGGATTAGGCCCTTTACAATGCCTCTCATGAAGCATGCTTGTACTGCACTGGAAAGAGTTCCTCTCACACGGCCAAAGGTTATAGATTGTTTGATGAAGAAATTCAATCAAGATTTAGTCTTCTGTCGCGCCCCGAACGACAATGATATCACAAGTGTTGTCCATGACCGACAAGTGGAAAAAATGGATCCTTTGCTTGATTGGGTGAAGTCAGAATTTGGCTTCAAGCCTGCTGTGTACTCAAGCTTTTTTGGTGGGAAGCAGGAGGATGGCCTCACAAAGGCTATAGAGACCTTACTAAAACAAACAGATGACTGTGAATTGGCAGCAATTGACGCCATCGCATCATCAGCACACTCGCTAATAATTGCTATTGGAATTTTTCGCGGGAAGTTACAGATTGAAGAAGCAATCGAGTTGATCAGACTCGAAGAAGATATACAGGTGGATAGATGGGGTTTGGTTGAAGGCGGTCATGATGTTGATGCAGCTGATCTCAGGGTACAGATCTCATCAGCTGCTGTATTCTTAGGTCTGTCAAGAAGGATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0008150 GO:0009987 GO:0016043 GO:0016604 GO:0016607 GO:0022607 GO:0031974 GO:0031981 GO:0034622 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043461 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0065003 GO:0070013 GO:0070071 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ATP12

Protein Analysis

340

Amino Acids

37.27

Weight (kDa)

6.26

Isoelectric Point (pI)

49.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATP12	PF07542	101 - 225	3.3e-30	ATP12 chaperone protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005675)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G40660
fragaria_vesca	FvH4_6g52290 FvH4_6g52310
malus_domestica	MD09G1019000.v1.1 MD17G1019600.v1.1
prunus_persica	Prupe.3G298100_v2.0.a1
pyrus_communis	pycom111g01580 pycom17g01620
rosa_chinensis	RchiOBHm_Chr2g0173821 RchiOBHm_Chr2g0174241
rosa_laevigata	RLG00000022226 RLG00000022231
rosa_multiflora	Rmu_sc0003188.1_g000011 Rmu_sc0037316.1_g000001
rosa_roxburghii	Rroxscaffold_2G00078300 Rroxscaffold_2G00078590 Rroxscaffold_4G00327960
rosa_rugosa	Rorug02G0573100 Rorug02G0573500
rosa_samantha	Rh2AG652100 Rh2AG652300 Rh2AG656100 Rh2BG662900 Rh2BG663100 Rh2BG667300 Rh2CG627500 Rh2DG678000
rosa_wichuraiana	Rw2G053550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	597, 855
AciI	CCGC	6 cut(s)	96, 102, 248, 440, 855, 939
AclWI	GGATC	3 cut(s)	67, 650, 663
AcoI	YGGCCR	2 cut(s)	333, 539
AcsI	RAATTY	4 cut(s)	7, 569, 683, 846
AcuI	CTGAAG	1 cut(s)	70
AcyI	GRCGYC	2 cut(s)	251, 804
AfaI	GTAC	3 cut(s)	511, 707, 972
AfiI	CCNNNNNNNGG	3 cut(s)	430, 643, 748
AgsI	TTSAA	4 cut(s)	574, 694, 872, 935
AjnI	CCWGG	2 cut(s)	423, 456
AluBI	AGCT	7 cut(s)	77, 90, 234, 409, 714, 959, 986
AluI	AGCT	7 cut(s)	77, 90, 234, 409, 714, 959, 986
Alw21I	GWGCWC	1 cut(s)	79
Alw26I	GTCTC	1 cut(s)	752
AlwI	GGATC	3 cut(s)	67, 650, 663
AlwNI	CAGNNNCTG	1 cut(s)	989
Ama87I	CYCGRG	1 cut(s)	72
AoxI	GGCC	7 cut(s)	130, 329, 333, 398, 476, 539, 739
ApeKI	GCWGC	4 cut(s)	115, 794, 956, 986
ApoI	RAATTY	4 cut(s)	7, 569, 683, 846
Asp700I	GAANNNNTTC	2 cut(s)	525, 569
AspLEI	GCGC	1 cut(s)	599
AspS9I	GGNCC	4 cut(s)	131, 288, 399, 477
AsuHPI	GGTGA	4 cut(s)	139, 271, 331, 685
AvaI	CYCGRG	1 cut(s)	72
AvaII	GGWCC	1 cut(s)	288
BaeGI	GKGCMC	1 cut(s)	193
BamHI	GGATCC	1 cut(s)	655
BanII	GRGCYC	1 cut(s)	79
BarI	GAAGNNNNNNTAC	2 cut(s)	891, 923
BbsI	GAAGAC	2 cut(s)	177, 580
Bbv12I	GWGCWC	1 cut(s)	79
BbvI	GCAGC	4 cut(s)	127, 806, 968, 973
BccI	CCATC	8 cut(s)	142, 299, 335, 397, 461, 731, 815, 915
BceAI	ACGGC	3 cut(s)	145, 205, 554
BciT130I	CCWGG	2 cut(s)	425, 458
BclI	TGATCA	2 cut(s)	15, 888
BcoDI	GTCTC	1 cut(s)	752
BfmI	CTRYAG	1 cut(s)	753
BglII	AGATCT	1 cut(s)	975
BisI	GCNGC	6 cut(s)	116, 249, 441, 795, 957, 987
BlsI	GCNGC	6 cut(s)	117, 250, 442, 796, 958, 988
Bme1390I	CCNGG	2 cut(s)	425, 458
Bme18I	GGWCC	1 cut(s)	288
BmeT110I	CYCGRG	1 cut(s)	72
BmgT120I	GGNCC	4 cut(s)	131, 288, 399, 477
BmiI	GGNNCC	3 cut(s)	289, 401, 657
BmrFI	CCNGG	2 cut(s)	425, 458
BmsI	GCATC	4 cut(s)	255, 351, 821, 943
BpiI	GAAGAC	2 cut(s)	177, 580
BplI	GAGNNNNNCTC	2 cut(s)	516, 548
BpuEI	CTTGAG	1 cut(s)	694
BsaHI	GRCGYC	2 cut(s)	251, 804
BsaI	GGTCTC	1 cut(s)	752
BsaJI	CCNNGG	2 cut(s)	156, 423
BsaWI	WCCGGW	1 cut(s)	372
Bsc4I	CCNNNNNNNGG	3 cut(s)	430, 643, 748
Bse118I	RCCGGY	1 cut(s)	331
Bse1I	ACTGG	1 cut(s)	522
Bse3DI	GCAATG	1 cut(s)	352
BseBI	CCWGG	2 cut(s)	425, 458
BseDI	CCNNGG	2 cut(s)	156, 423
BseGI	GGATG	1 cut(s)	742
BseLI	CCNNNNNNNGG	3 cut(s)	430, 643, 748
BseMI	GCAATG	1 cut(s)	352
BseMII	CTCAG	1 cut(s)	979
BseNI	ACTGG	1 cut(s)	522
BseRI	GAGGAG	1 cut(s)	145
BseSI	GKGCMC	1 cut(s)	193
BseX3I	CGGCCG	1 cut(s)	333
BseXI	GCAGC	4 cut(s)	127, 806, 968, 973
BsgI	GTGCAG	1 cut(s)	498
Bsh1236I	CGCG	2 cut(s)	597, 855
Bsh1285I	CGRYCG	1 cut(s)	336
BshFI	GGCC	7 cut(s)	132, 331, 335, 400, 478, 541, 741
BsiEI	CGRYCG	1 cut(s)	336
BsiHKAI	GWGCWC	1 cut(s)	79
BsiHKCI	CYCGRG	1 cut(s)	72
BsiSI	CCGG	2 cut(s)	332, 373
BslI	CCNNNNNNNGG	3 cut(s)	430, 643, 748
BsmAI	GTCTC	1 cut(s)	752
BsnI	GGCC	7 cut(s)	132, 331, 335, 400, 478, 541, 741
Bso31I	GGTCTC	1 cut(s)	752
BsoBI	CYCGRG	1 cut(s)	72
Bsp1286I	GDGCHC	2 cut(s)	79, 193
Bsp143I	GATC	7 cut(s)	15, 54, 59, 655, 888, 961, 975
BspACI	CCGC	6 cut(s)	96, 102, 248, 440, 855, 939
BspANI	GGCC	7 cut(s)	132, 331, 335, 400, 478, 541, 741
BspCNI	CTCAG	1 cut(s)	978
BspFNI	CGCG	2 cut(s)	597, 855
BspHI	TCATGA	3 cut(s)	279, 495, 943
BspLI	GGNNCC	3 cut(s)	289, 401, 657
BspPI	GGATC	3 cut(s)	67, 650, 663
BspTNI	GGTCTC	1 cut(s)	752
BsrDI	GCAATG	1 cut(s)	352
BsrFI	RCCGGY	1 cut(s)	331
BsrI	ACTGG	1 cut(s)	522
BssAI	RCCGGY	1 cut(s)	331
BssECI	CCNNGG	2 cut(s)	156, 423
BssMI	GATC	7 cut(s)	15, 54, 59, 655, 888, 961, 975
BssNI	GRCGYC	2 cut(s)	251, 804
Bst2UI	CCWGG	2 cut(s)	425, 458
Bst4CI	ACNGT	2 cut(s)	358, 785
Bst6I	CTCTTC	2 cut(s)	73, 389
BstACI	GRCGYC	2 cut(s)	251, 804
BstC8I	GCNNGC	4 cut(s)	79, 333, 504, 699
BstDEI	CTNAG	2 cut(s)	965, 997
BstENI	CCTNNNNNAGG	2 cut(s)	428, 746
BstF5I	GGATG	1 cut(s)	742
BstFNI	CGCG	2 cut(s)	597, 855
BstHHI	GCGC	1 cut(s)	599
BstKTI	GATC	7 cut(s)	18, 57, 62, 658, 891, 964, 978
BstMAI	GTCTC	1 cut(s)	752
BstMBI	GATC	7 cut(s)	15, 54, 59, 655, 888, 961, 975
BstMCI	CGRYCG	1 cut(s)	336
BstMWI	GCNNNNNNNGC	7 cut(s)	87, 240, 406, 440, 512, 803, 818
BstNI	CCWGG	2 cut(s)	425, 458
BstNSI	RCATGY	1 cut(s)	506
BstSCI	CCNGG	2 cut(s)	423, 456
BstSFI	CTRYAG	1 cut(s)	753
BstSLI	GKGCMC	1 cut(s)	193
BstUI	CGCG	2 cut(s)	597, 855
BstV1I	GCAGC	4 cut(s)	127, 806, 968, 973
BstV2I	GAAGAC	2 cut(s)	177, 580
BstX2I	RGATCY	3 cut(s)	59, 655, 975
BstYI	RGATCY	3 cut(s)	59, 655, 975
BstZI	CGGCCG	1 cut(s)	333
BsuRI	GGCC	7 cut(s)	132, 331, 335, 400, 478, 541, 741
BtgZI	GCGATG	1 cut(s)	793
BtsCI	GGATG	1 cut(s)	742
BtsI	GCAGTG	1 cut(s)	193
BtsIMutI	CAGTG	2 cut(s)	193, 515
Cac8I	GCNNGC	4 cut(s)	79, 333, 504, 699
CaiI	CAGNNNCTG	1 cut(s)	989
CciI	TCATGA	3 cut(s)	279, 495, 943
CfoI	GCGC	1 cut(s)	599
Cfr10I	RCCGGY	1 cut(s)	331
Cfr13I	GGNCC	4 cut(s)	131, 288, 399, 477
CseI	GACGC	2 cut(s)	240, 812
Csp6I	GTAC	3 cut(s)	510, 706, 971
CviAII	CATG	5 cut(s)	280, 496, 503, 632, 944
CviQI	GTAC	3 cut(s)	510, 706, 971
DdeI	CTNAG	2 cut(s)	965, 997
DpnI	GATC	7 cut(s)	17, 56, 61, 657, 890, 963, 977
DpnII	GATC	7 cut(s)	15, 54, 59, 655, 888, 961, 975
EaeI	YGGCCR	2 cut(s)	333, 539
EagI	CGGCCG	1 cut(s)	333
Eam1104I	CTCTTC	2 cut(s)	73, 389
EarI	CTCTTC	2 cut(s)	73, 389
Ecl136II	GAGCTC	1 cut(s)	77
EclXI	CGGCCG	1 cut(s)	333
Eco24I	GRGCYC	1 cut(s)	79
Eco31I	GGTCTC	1 cut(s)	752
Eco32I	GATATC	1 cut(s)	616
Eco47I	GGWCC	1 cut(s)	288
Eco52I	CGGCCG	1 cut(s)	333
Eco53kI	GAGCTC	1 cut(s)	77
Eco57I	CTGAAG	1 cut(s)	70
Eco88I	CYCGRG	1 cut(s)	72
EcoICRI	GAGCTC	1 cut(s)	77
EcoNI	CCTNNNNNAGG	2 cut(s)	428, 746
EcoO109I	RGGNCCY	2 cut(s)	399, 477
EcoRII	CCWGG	2 cut(s)	423, 456
EcoRV	GATATC	1 cut(s)	616
EcoT38I	GRGCYC	1 cut(s)	79
FaeI	CATG	5 cut(s)	283, 499, 506, 635, 947
FatI	CATG	5 cut(s)	279, 495, 502, 631, 943
FauI	CCCGC	1 cut(s)	848
FbaI	TGATCA	2 cut(s)	15, 888
Fnu4HI	GCNGC	6 cut(s)	116, 249, 441, 795, 957, 987
FokI	GGATG	1 cut(s)	749
FriOI	GRGCYC	1 cut(s)	79
FseI	GGCCGGCC	1 cut(s)	335
Fsp4HI	GCNGC	6 cut(s)	116, 249, 441, 795, 957, 987
GlaI	GCGC	1 cut(s)	598
GluI	GCNGC	6 cut(s)	116, 249, 441, 795, 957, 987
HaeIII	GGCC	7 cut(s)	132, 331, 335, 400, 478, 541, 741
HapII	CCGG	2 cut(s)	332, 373
HgaI	GACGC	2 cut(s)	240, 812
HhaI	GCGC	1 cut(s)	599
Hin1I	GRCGYC	2 cut(s)	251, 804
Hin1II	CATG	5 cut(s)	283, 499, 506, 635, 947
Hin6I	GCGC	1 cut(s)	597
HinP1I	GCGC	1 cut(s)	597
HindIII	AAGCTT	2 cut(s)	407, 712
HinfI	GANTC	1 cut(s)	894
HpaII	CCGG	2 cut(s)	332, 373
HphI	GGTGA	4 cut(s)	139, 271, 331, 685
Hpy166II	GTNNAC	1 cut(s)	706
Hpy188I	TCNGA	6 cut(s)	41, 59, 127, 160, 682, 893
Hpy188III	TCNNGA	6 cut(s)	72, 280, 496, 578, 944, 1008
Hpy8I	GTNNAC	1 cut(s)	706
Hpy99I	CGWCG	6 cut(s)	131, 226, 256, 259, 328, 341
HpyAV	CCTTC	5 cut(s)	94, 160, 424, 929, 1005
HpyCH4III	ACNGT	2 cut(s)	358, 785
HpyCH4IV	ACGT	1 cut(s)	210
HpyCH4V	TGCA	2 cut(s)	515, 956
HpyF10VI	GCNNNNNNNGC	7 cut(s)	87, 240, 406, 440, 512, 803, 818
HpyF3I	CTNAG	2 cut(s)	965, 997
HpySE526I	ACGT	1 cut(s)	210
Hsp92I	GRCGYC	2 cut(s)	251, 804
Hsp92II	CATG	5 cut(s)	283, 499, 506, 635, 947
HspAI	GCGC	1 cut(s)	597
KroI	GCCGGC	1 cut(s)	331
KroNI	GCCGGC	1 cut(s)	333
Ksp22I	TGATCA	2 cut(s)	15, 888
Kzo9I	GATC	7 cut(s)	15, 54, 59, 655, 888, 961, 975
LmnI	GCTCC	2 cut(s)	95, 239
Lsp1109I	GCAGC	4 cut(s)	127, 806, 968, 973
LweI	GCATC	4 cut(s)	255, 351, 821, 943
MaeII	ACGT	1 cut(s)	210
MaeIII	GTNAC	3 cut(s)	259, 319, 861
MalI	GATC	7 cut(s)	17, 56, 61, 657, 890, 963, 977
MboI	GATC	7 cut(s)	15, 54, 59, 655, 888, 961, 975
MboII	GAAGA	9 cut(s)	60, 129, 182, 406, 577, 580, 884, 911, 914
MfeI	CAATTG	1 cut(s)	798
MflI	RGATCY	3 cut(s)	59, 655, 975
MhlI	GDGCHC	2 cut(s)	79, 193
MluCI	AATT	7 cut(s)	7, 569, 683, 788, 798, 834, 846
MlyI	GAGTC	1 cut(s)	888
MmeI	TCCRAC	3 cut(s)	19, 150, 332
MnlI	CCTC	8 cut(s)	56, 166, 321, 390, 501, 540, 727, 752
MroNI	GCCGGC	1 cut(s)	331
MroXI	GAANNNNTTC	2 cut(s)	525, 569
MseI	TTAA	1 cut(s)	1018
MspA1I	CMGCKG	2 cut(s)	959, 986
MspI	CCGG	2 cut(s)	332, 373
MspR9I	CCNGG	2 cut(s)	425, 458
MunI	CAATTG	1 cut(s)	798
MvaI	CCWGG	2 cut(s)	425, 458
MvnI	CGCG	2 cut(s)	597, 855
MwoI	GCNNNNNNNGC	7 cut(s)	87, 240, 406, 440, 512, 803, 818
NaeI	GCCGGC	1 cut(s)	333
NdeII	GATC	7 cut(s)	15, 54, 59, 655, 888, 961, 975
NgoMIV	GCCGGC	1 cut(s)	331
NlaIII	CATG	5 cut(s)	283, 499, 506, 635, 947
NlaIV	GGNNCC	3 cut(s)	289, 401, 657
NmuCI	GTSAC	2 cut(s)	259, 319
NspI	RCATGY	1 cut(s)	506
PaeI	GCATGC	1 cut(s)	506
PaeR7I	CTCGAG	1 cut(s)	72
PagI	TCATGA	3 cut(s)	279, 495, 943
PcsI	WCGNNNNNNNCGW	1 cut(s)	260
PdiI	GCCGGC	1 cut(s)	333
PdmI	GAANNNNTTC	2 cut(s)	525, 569
PflFI	GACNNNGTC	1 cut(s)	286
PkrI	GCNGC	6 cut(s)	117, 250, 442, 796, 958, 988
PleI	GAGTC	1 cut(s)	888
PpsI	GAGTC	1 cut(s)	888
Psp124BI	GAGCTC	1 cut(s)	79
Psp6I	CCWGG	2 cut(s)	423, 456
PspGI	CCWGG	2 cut(s)	423, 456
PspN4I	GGNNCC	3 cut(s)	289, 401, 657
PspPI	GGNCC	4 cut(s)	131, 288, 399, 477
PstNI	CAGNNNCTG	1 cut(s)	989
PsuI	RGATCY	3 cut(s)	59, 655, 975
PsyI	GACNNNGTC	1 cut(s)	286
PvuII	CAGCTG	2 cut(s)	959, 986
RigI	GGCCGGCC	1 cut(s)	335
RsaI	GTAC	3 cut(s)	511, 707, 972
RsaNI	GTAC	3 cut(s)	510, 706, 971
SacI	GAGCTC	1 cut(s)	79
SaqAI	TTAA	1 cut(s)	1018
SatI	GCNGC	6 cut(s)	116, 249, 441, 795, 957, 987
Sau3AI	GATC	7 cut(s)	15, 54, 59, 655, 888, 961, 975
Sau96I	GGNCC	4 cut(s)	131, 288, 399, 477
SchI	GAGTC	1 cut(s)	888
ScrFI	CCNGG	2 cut(s)	425, 458
SduI	GDGCHC	2 cut(s)	79, 193
SfaNI	GCATC	4 cut(s)	255, 351, 821, 943
SfcI	CTRYAG	1 cut(s)	753
Sfr274I	CTCGAG	1 cut(s)	72
SinI	GGWCC	1 cut(s)	288
SlaI	CTCGAG	1 cut(s)	72
SmlI	CTYRAG	2 cut(s)	72, 709
SmoI	CTYRAG	2 cut(s)	72, 709
SphI	GCATGC	1 cut(s)	506
Sse9I	AATT	7 cut(s)	7, 569, 683, 788, 798, 834, 846
SsiI	CCGC	6 cut(s)	96, 102, 248, 440, 855, 939
SstI	GAGCTC	1 cut(s)	79
StyD4I	CCNGG	2 cut(s)	423, 456
TaaI	ACNGT	2 cut(s)	358, 785
TaiI	ACGT	1 cut(s)	213
TaqI	TCGA	6 cut(s)	73, 111, 224, 392, 882, 897
TaqII	GACCGA	1 cut(s)	651
TasI	AATT	7 cut(s)	7, 569, 683, 788, 798, 834, 846
TatI	WGTACW	2 cut(s)	509, 705
TauI	GCSGC	2 cut(s)	251, 443
Tru1I	TTAA	1 cut(s)	1018
Tru9I	TTAA	1 cut(s)	1018
TscAI	CASTG	2 cut(s)	193, 522
TseFI	GTSAC	2 cut(s)	259, 319
TseI	GCWGC	4 cut(s)	115, 794, 956, 986
Tsp45I	GTSAC	2 cut(s)	259, 319
TspDTI	ATGAA	3 cut(s)	268, 512, 578
TspGWI	ACGGA	1 cut(s)	177
TspRI	CASTG	2 cut(s)	193, 522
Tth111I	GACNNNGTC	1 cut(s)	286
VpaK11BI	GGWCC	1 cut(s)	288
XagI	CCTNNNNNAGG	2 cut(s)	428, 746
XapI	RAATTY	4 cut(s)	7, 569, 683, 846
XceI	RCATGY	1 cut(s)	506
XcmI	CCANNNNNNNNNTGG	1 cut(s)	464
XhoI	CTCGAG	1 cut(s)	72
XmnI	GAANNNNTTC	2 cut(s)	525, 569

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.