MD17G1019600.v1.1

ATP synthase mitochondrial F1 complex assembly factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005675
Physical Location & Seq
Forward (+)
1502029 .. 1503960
1932 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1019600.v1.1.491

Sequence Viewer

Length: 498 bp
ATGCTCGATTACCGGACGCTCAAAACGCCATCGAAGAGGCCCCTAAAGCTTCCTACTTTAGCTCTGGCTAAGGCCATTGCCGCGGAATGGGAATACCAGCCCGAATTTCATGTAGACCGACAAGTGGAGAAAATGGATCCTTTGCTTGATTGGGTGAAGTCAGAATTTGGCTTCAAGCCTGCTGTGTACTCCAGTTTTTTTGGTGGGAAGCAGGAGGATGGTCTCACAAAGGCTATAGAGACCTTACTGAAACAAACAGATGACTGTGAATTGGCAGCAATTGGTGCCATTGCATCATCAGCACACTCTCTAATAATTGCTATCGGAATTTTTCGTGGGAAGTTACAGATTGAAGAAGCAATCGAGTTGATCAGACTCGAAGAAGATATACAGGTAGACAGGTGGGGTTTGGTTGAAGGTGGTCATGATGTAGATGCAGCTGATTTCAGGGTACAGATCTCATCGGCTGCTGTATTCCTAGGTCTGTCAAGGAGTTAA

Protein Analysis

166

Amino Acids

18.33

Weight (kDa)

5.0

Isoelectric Point (pI)

39.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP12 PF07542 1 - 35 1.2e-08 ATP12 chaperone protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 284
AccI GTMKAC 2 cut(s) 114, 396
AccII CGCG 1 cut(s) 83
AciI CCGC 2 cut(s) 81, 83
AclWI GGATC 2 cut(s) 131, 144
AcsI RAATTY 3 cut(s) 104, 164, 327
AfaI GTAC 2 cut(s) 188, 453
AfiI CCNNNNNNNGG 2 cut(s) 87, 124
AgsI TTSAA 3 cut(s) 175, 353, 416
AluBI AGCT 3 cut(s) 49, 62, 440
AluI AGCT 3 cut(s) 49, 62, 440
Alw26I GTCTC 2 cut(s) 227, 233
AlwI GGATC 2 cut(s) 131, 144
AoxI GGCC 2 cut(s) 38, 72
ApeKI GCWGC 3 cut(s) 275, 437, 467
ApoI RAATTY 3 cut(s) 104, 164, 327
AspA2I CCTAGG 1 cut(s) 478
AspS9I GGNCC 1 cut(s) 39
AsuHPI GGTGA 1 cut(s) 166
AvrII CCTAGG 1 cut(s) 478
BamHI GGATCC 1 cut(s) 136
BanI GGYRCC 1 cut(s) 284
BarI GAAGNNNNNNTAC 2 cut(s) 372, 404
BbvI GCAGC 3 cut(s) 287, 449, 454
BccI CCATC 2 cut(s) 37, 212
BclI TGATCA 1 cut(s) 369
BcoDI GTCTC 2 cut(s) 227, 233
BfaI CTAG 1 cut(s) 479
BfmI CTRYAG 1 cut(s) 234
BglII AGATCT 1 cut(s) 456
BisI GCNGC 4 cut(s) 81, 276, 438, 468
BlnI CCTAGG 1 cut(s) 478
BlsI GCNGC 4 cut(s) 82, 277, 439, 469
BmgT120I GGNCC 1 cut(s) 39
BmiI GGNNCC 3 cut(s) 41, 138, 286
BmsI GCATC 2 cut(s) 302, 424
BpmI CTGGAG 1 cut(s) 175
Bpu10I CCTNAGC 1 cut(s) 69
BsaI GGTCTC 2 cut(s) 227, 233
BsaJI CCNNGG 2 cut(s) 81, 478
BsaWI WCCGGW 1 cut(s) 12
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 124
Bse1I ACTGG 1 cut(s) 192
Bse3DI GCAATG 2 cut(s) 75, 288
BseDI CCNNGG 2 cut(s) 81, 478
BseGI GGATG 1 cut(s) 223
BseLI CCNNNNNNNGG 2 cut(s) 87, 124
BseMI GCAATG 2 cut(s) 75, 288
BseNI ACTGG 1 cut(s) 192
BseXI GCAGC 3 cut(s) 287, 449, 454
Bsh1236I CGCG 1 cut(s) 83
BshFI GGCC 2 cut(s) 40, 74
BshNI GGYRCC 1 cut(s) 284
BsiSI CCGG 1 cut(s) 13
BslI CCNNNNNNNGG 2 cut(s) 87, 124
BsmAI GTCTC 2 cut(s) 227, 233
BsnI GGCC 2 cut(s) 40, 74
Bso31I GGTCTC 2 cut(s) 227, 233
Bsp143I GATC 3 cut(s) 136, 369, 456
BspACI CCGC 2 cut(s) 81, 83
BspANI GGCC 2 cut(s) 40, 74
BspFNI CGCG 1 cut(s) 83
BspHI TCATGA 1 cut(s) 424
BspLI GGNNCC 3 cut(s) 41, 138, 286
BspPI GGATC 2 cut(s) 131, 144
BspT107I GGYRCC 1 cut(s) 284
BspTNI GGTCTC 2 cut(s) 227, 233
BsrDI GCAATG 2 cut(s) 75, 288
BsrI ACTGG 1 cut(s) 192
BssECI CCNNGG 2 cut(s) 81, 478
BssMI GATC 3 cut(s) 136, 369, 456
BssT1I CCWWGG 1 cut(s) 478
Bst4CI ACNGT 1 cut(s) 266
Bst6I CTCTTC 1 cut(s) 29
BstAPI GCANNNNNTGC 1 cut(s) 284
BstC8I GCNNGC 1 cut(s) 180
BstDEI CTNAG 1 cut(s) 69
BstDSI CCRYGG 1 cut(s) 81
BstF5I GGATG 1 cut(s) 223
BstFNI CGCG 1 cut(s) 83
BstKTI GATC 3 cut(s) 139, 372, 459
BstMAI GTCTC 2 cut(s) 227, 233
BstMBI GATC 3 cut(s) 136, 369, 456
BstMWI GCNNNNNNNGC 5 cut(s) 25, 46, 80, 284, 299
BstSFI CTRYAG 1 cut(s) 234
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 3 cut(s) 287, 449, 454
BstX2I RGATCY 2 cut(s) 136, 456
BstYI RGATCY 2 cut(s) 136, 456
BsuRI GGCC 2 cut(s) 40, 74
BtgI CCRYGG 1 cut(s) 81
BtsCI GGATG 1 cut(s) 223
Cac8I GCNNGC 1 cut(s) 180
CciI TCATGA 1 cut(s) 424
Cfr13I GGNCC 1 cut(s) 39
Cfr42I CCGCGG 1 cut(s) 84
CseI GACGC 1 cut(s) 25
Csp6I GTAC 2 cut(s) 187, 452
CviAII CATG 2 cut(s) 110, 425
CviQI GTAC 2 cut(s) 187, 452
DdeI CTNAG 1 cut(s) 69
DpnI GATC 3 cut(s) 138, 371, 458
DpnII GATC 3 cut(s) 136, 369, 456
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Eco130I CCWWGG 1 cut(s) 478
Eco31I GGTCTC 2 cut(s) 227, 233
EcoO109I RGGNCCY 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 478
ErhI CCWWGG 1 cut(s) 478
FaeI CATG 2 cut(s) 113, 428
FaiI YATR 4 cut(s) 111, 236, 389, 426
FatI CATG 2 cut(s) 109, 424
FbaI TGATCA 1 cut(s) 369
FblI GTMKAC 2 cut(s) 114, 396
Fnu4HI GCNGC 4 cut(s) 81, 276, 438, 468
FokI GGATG 1 cut(s) 230
Fsp4HI GCNGC 4 cut(s) 81, 276, 438, 468
FspBI CTAG 1 cut(s) 479
GluI GCNGC 4 cut(s) 81, 276, 438, 468
GsuI CTGGAG 1 cut(s) 175
HaeIII GGCC 2 cut(s) 40, 74
HapII CCGG 1 cut(s) 13
HgaI GACGC 1 cut(s) 25
Hin1II CATG 2 cut(s) 113, 428
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 1 cut(s) 375
HpaII CCGG 1 cut(s) 13
HphI GGTGA 1 cut(s) 166
Hpy166II GTNNAC 3 cut(s) 115, 187, 397
Hpy188I TCNGA 3 cut(s) 163, 326, 374
Hpy188III TCNNGA 1 cut(s) 425
Hpy8I GTNNAC 3 cut(s) 115, 187, 397
HpyAV CCTTC 1 cut(s) 410
HpyCH4III ACNGT 1 cut(s) 266
HpyCH4V TGCA 2 cut(s) 293, 437
HpyF10VI GCNNNNNNNGC 5 cut(s) 25, 46, 80, 284, 299
HpyF3I CTNAG 1 cut(s) 69
Hsp92II CATG 2 cut(s) 113, 428
Ksp22I TGATCA 1 cut(s) 369
KspI CCGCGG 1 cut(s) 84
Kzo9I GATC 3 cut(s) 136, 369, 456
LpnPI CCDG 9 cut(s) 26, 50, 110, 192, 197, 205, 377, 385, 433
Lsp1109I GCAGC 3 cut(s) 287, 449, 454
LweI GCATC 2 cut(s) 302, 424
MaeI CTAG 1 cut(s) 479
MaeIII GTNAC 1 cut(s) 342
MalI GATC 3 cut(s) 138, 371, 458
MboI GATC 3 cut(s) 136, 369, 456
MboII GAAGA 4 cut(s) 46, 365, 392, 395
MfeI CAATTG 1 cut(s) 279
MflI RGATCY 2 cut(s) 136, 456
MluCI AATT 6 cut(s) 104, 164, 269, 279, 315, 327
MlyI GAGTC 1 cut(s) 369
MnlI CCTC 2 cut(s) 30, 208
MseI TTAA 1 cut(s) 496
MspA1I CMGCKG 2 cut(s) 83, 440
MspI CCGG 1 cut(s) 13
MunI CAATTG 1 cut(s) 279
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 5 cut(s) 25, 46, 80, 284, 299
NdeII GATC 3 cut(s) 136, 369, 456
NlaIII CATG 2 cut(s) 113, 428
NlaIV GGNNCC 3 cut(s) 41, 138, 286
PagI TCATGA 1 cut(s) 424
PkrI GCNGC 4 cut(s) 82, 277, 439, 469
PleI GAGTC 1 cut(s) 369
PpsI GAGTC 1 cut(s) 369
PspN4I GGNNCC 3 cut(s) 41, 138, 286
PspPI GGNCC 1 cut(s) 39
PsuI RGATCY 2 cut(s) 136, 456
PvuII CAGCTG 1 cut(s) 440
RsaI GTAC 2 cut(s) 188, 453
RsaNI GTAC 2 cut(s) 187, 452
SacII CCGCGG 1 cut(s) 84
SaqAI TTAA 1 cut(s) 496
SatI GCNGC 4 cut(s) 81, 276, 438, 468
Sau3AI GATC 3 cut(s) 136, 369, 456
Sau96I GGNCC 1 cut(s) 39
SchI GAGTC 1 cut(s) 369
SetI ASST 8 cut(s) 51, 64, 245, 396, 404, 421, 442, 484
SfaNI GCATC 2 cut(s) 302, 424
SfcI CTRYAG 1 cut(s) 234
Sfr303I CCGCGG 1 cut(s) 84
SgrBI CCGCGG 1 cut(s) 84
Sse9I AATT 6 cut(s) 104, 164, 269, 279, 315, 327
SsiI CCGC 2 cut(s) 81, 83
SspMI CTAG 1 cut(s) 479
StyI CCWWGG 1 cut(s) 478
TaaI ACNGT 1 cut(s) 266
TaqI TCGA 4 cut(s) 6, 32, 363, 378
TaqII GACCGA 1 cut(s) 132
TasI AATT 6 cut(s) 104, 164, 269, 279, 315, 327
TatI WGTACW 1 cut(s) 186
TauI GCSGC 1 cut(s) 83
Tru1I TTAA 1 cut(s) 496
Tru9I TTAA 1 cut(s) 496
TseI GCWGC 3 cut(s) 275, 437, 467
TspDTI ATGAA 1 cut(s) 98
XapI RAATTY 3 cut(s) 104, 164, 327
XmaJI CCTAGG 1 cut(s) 478
XmiI GTMKAC 2 cut(s) 114, 396
XspI CTAG 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.