RLG00000022226

ATP synthase mitochondrial F1 complex assembly factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005675
Physical Location & Seq
Reverse (-)
81818324 .. 81820561
2238 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022226

Sequence Viewer

Length: 1002 bp
ATGGCCAATTTCCTGATAAACAGAACCCTAAAATCAATCCCAAACCTCAACCTAATCACCACCGCCCTATGCTCTCGTTCCCGCCAATTCAGCTCCGCCGCTGCCACCGCCGCCGAGCAACCCCAGTCCGACGGCGGCGAGTCTTCCTCCTCCTCCTTCACCTTCTCATCCGACGGCCAAGACAGCATACACATCAAGCCCTCTTCTTCCGTTCCAAAGCGCGAAACGGCGTCGTCGTCGGTGACGATGCCGATGTCGTTCATGACAGGGTCGGTGGTGGGGAAGAGGTTCTACACTCAAGTGACGACGAGGAAGGCCGACGACGGGAATGGATGGTCGGTGATGCTTGATTATAGGACGCTCAAGACTCCATCAAAGAGACCCTTAAAGCTTCCGACTTTGGGTTTAGCTAAGGCCATTGCTGCTGAGTGGGAATACCAGGAAACAGATGGGATTAGGCCCTTCACGATGCCTCTCATGAAGCTTGCTTGTACTGCACTTGAAAGAGTTCCTATCACACGGCATAAGGTTATAGAATACTTGATAAATAGATTCAATCAAGATTTAGTCTTTTGTCGTGCCCCGGATGACAGTGAGCTGACAAGTGGCATCCATGAACGGCAAGTGGAAAAGATTGATCCATTACTTGATTGGCTGGAGTCCGAATTTGGCTTTAAGCCTGTTGTTTACTCCAGCTTTTTTGGTGGAAAGCAGGAGGATGGACTTCTAAAAGCTGTAGAAACCCTACTGAAGAAAACAGATGAGTGTGAATTGGCAGCAATTGATGCTATTGCAGGAGCAGCACACTCCCTAACAATTGCTCTTGGATTGTTTCGTGGGAAACTGCAGATTGAAGAAGCAATAGAGCTAATTAGACTTGAAGAAGATTTTCAGGTAGACAAGTGGGGTCTGGTTGAAGGTGGTCATGATGTAGATATTGCTGATCTCAAAGTACAGATCGCATCAGCTGCTGTATTCCTAGGTCTTTCAAGGAGGATTTAA

Protein Analysis

334

Amino Acids

36.68

Weight (kDa)

5.68

Isoelectric Point (pI)

48.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP12 PF07542 95 - 220 7.9e-31 ATP12 chaperone protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 897
AccII CGCG 1 cut(s) 222
AciI CCGC 7 cut(s) 63, 82, 96, 99, 108, 111, 135
AclWI GGATC 1 cut(s) 632
AcoI YGGCCR 2 cut(s) 3, 175
AcsI RAATTY 1 cut(s) 665
AcuI CTGAAG 1 cut(s) 770
AcyI GRCGYC 1 cut(s) 230
AfaI GTAC 2 cut(s) 493, 954
AfiI CCNNNNNNNGG 2 cut(s) 324, 401
AgsI TTSAA 6 cut(s) 503, 556, 854, 881, 917, 990
AjnI CCWGG 1 cut(s) 438
AleI CACNNNNGTG 1 cut(s) 299
AluBI AGCT 9 cut(s) 93, 391, 410, 484, 598, 696, 734, 868, 968
AluI AGCT 9 cut(s) 93, 391, 410, 484, 598, 696, 734, 868, 968
Alw26I GTCTC 1 cut(s) 373
AlwI GGATC 1 cut(s) 632
AlwNI CAGNNNCTG 1 cut(s) 971
AoxI GGCC 5 cut(s) 3, 175, 315, 414, 458
ApeKI GCWGC 5 cut(s) 101, 422, 776, 800, 968
ApoI RAATTY 1 cut(s) 665
Asp700I GAANNNNTTC 3 cut(s) 287, 507, 888
AspA2I CCTAGG 1 cut(s) 979
AspLEI GCGC 1 cut(s) 222
AspS9I GGNCC 1 cut(s) 459
AsuC2I CCSGG 1 cut(s) 584
AsuHPI GGTGA 4 cut(s) 49, 151, 253, 352
AvrII CCTAGG 1 cut(s) 979
BaeGI GKGCMC 1 cut(s) 583
BalI TGGCCA 1 cut(s) 5
BarI GAAGNNNNNNTAC 2 cut(s) 275, 307
BbsI GAAGAC 1 cut(s) 135
BbvI GCAGC 5 cut(s) 88, 409, 788, 812, 955
BccI CCATC 4 cut(s) 327, 379, 443, 713
BceAI ACGGC 5 cut(s) 148, 190, 243, 536, 635
BciT130I CCWGG 1 cut(s) 440
BcnI CCSGG 1 cut(s) 584
BcoDI GTCTC 1 cut(s) 373
BfaI CTAG 1 cut(s) 980
BfmI CTRYAG 2 cut(s) 735, 845
BisI GCNGC 8 cut(s) 99, 102, 111, 136, 423, 777, 801, 969
BlnI CCTAGG 1 cut(s) 979
BlsI GCNGC 8 cut(s) 100, 103, 112, 137, 424, 778, 802, 970
Bme1390I CCNGG 2 cut(s) 440, 584
BmgT120I GGNCC 1 cut(s) 459
BmrFI CCNGG 2 cut(s) 440, 584
BmrI ACTGGG 1 cut(s) 118
BmsI GCATC 6 cut(s) 237, 333, 459, 618, 775, 971
BmuI ACTGGG 1 cut(s) 118
BpiI GAAGAC 1 cut(s) 135
BplI GAGNNNNNCTC 2 cut(s) 131, 163
BpmI CTGGAG 2 cut(s) 676, 677
Bpu10I CCTNAGC 1 cut(s) 411
BpuEI CTTGAG 2 cut(s) 282, 347
BpuMI CCSGG 1 cut(s) 584
BsaHI GRCGYC 1 cut(s) 230
BsaI GGTCTC 1 cut(s) 373
BsaJI CCNNGG 2 cut(s) 582, 979
Bsc4I CCNNNNNNNGG 2 cut(s) 324, 401
Bse1I ACTGG 1 cut(s) 124
Bse3DI GCAATG 1 cut(s) 417
BseBI CCWGG 1 cut(s) 440
BseDI CCNNGG 2 cut(s) 582, 979
BseGI GGATG 5 cut(s) 167, 338, 592, 609, 724
BseLI CCNNNNNNNGG 2 cut(s) 324, 401
BseMI GCAATG 1 cut(s) 417
BseMII CTCAG 1 cut(s) 417
BseNI ACTGG 1 cut(s) 124
BseRI GAGGAG 2 cut(s) 139, 142
BseSI GKGCMC 1 cut(s) 583
BseXI GCAGC 5 cut(s) 88, 409, 788, 812, 955
BsgI GTGCAG 1 cut(s) 480
Bsh1236I CGCG 1 cut(s) 222
BshFI GGCC 5 cut(s) 5, 177, 317, 416, 460
BsiSI CCGG 1 cut(s) 584
BslI CCNNNNNNNGG 2 cut(s) 324, 401
BsmAI GTCTC 1 cut(s) 373
BsnI GGCC 5 cut(s) 5, 177, 317, 416, 460
Bso31I GGTCTC 1 cut(s) 373
Bsp1286I GDGCHC 1 cut(s) 583
Bsp143I GATC 3 cut(s) 637, 943, 957
BspACI CCGC 7 cut(s) 63, 82, 96, 99, 108, 111, 135
BspANI GGCC 5 cut(s) 5, 177, 317, 416, 460
BspCNI CTCAG 1 cut(s) 418
BspFNI CGCG 1 cut(s) 222
BspHI TCATGA 3 cut(s) 261, 477, 925
BspMAI CTGCAG 1 cut(s) 849
BspPI GGATC 1 cut(s) 632
BspTNI GGTCTC 1 cut(s) 373
BsrDI GCAATG 1 cut(s) 417
BsrI ACTGG 1 cut(s) 124
BssECI CCNNGG 2 cut(s) 582, 979
BssMI GATC 3 cut(s) 637, 943, 957
BssNI GRCGYC 1 cut(s) 230
BssT1I CCWWGG 1 cut(s) 979
Bst2UI CCWGG 1 cut(s) 440
Bst4CI ACNGT 1 cut(s) 593
Bst6I CTCTTC 2 cut(s) 208, 278
BstACI GRCGYC 1 cut(s) 230
BstAPI GCANNNNNTGC 2 cut(s) 785, 968
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 2 cut(s) 411, 426
BstF5I GGATG 5 cut(s) 167, 338, 592, 609, 724
BstFNI CGCG 1 cut(s) 222
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 3 cut(s) 640, 946, 960
BstMAI GTCTC 1 cut(s) 373
BstMBI GATC 3 cut(s) 637, 943, 957
BstMWI GCNNNNNNNGC 9 cut(s) 90, 107, 110, 183, 422, 494, 785, 800, 968
BstNI CCWGG 1 cut(s) 440
BstSCI CCNGG 2 cut(s) 438, 582
BstSFI CTRYAG 2 cut(s) 735, 845
BstSLI GKGCMC 1 cut(s) 583
BstUI CGCG 1 cut(s) 222
BstV1I GCAGC 5 cut(s) 88, 409, 788, 812, 955
BstV2I GAAGAC 1 cut(s) 135
BsuRI GGCC 5 cut(s) 5, 177, 317, 416, 460
BtsCI GGATG 5 cut(s) 167, 338, 592, 609, 724
BtsIMutI CAGTG 1 cut(s) 598
Cac8I GCNNGC 1 cut(s) 486
CaiI CAGNNNCTG 1 cut(s) 971
CciI TCATGA 3 cut(s) 261, 477, 925
CfoI GCGC 1 cut(s) 222
Cfr13I GGNCC 1 cut(s) 459
CseI GACGC 2 cut(s) 219, 367
Csp6I GTAC 2 cut(s) 492, 953
CviAII CATG 4 cut(s) 262, 478, 614, 926
CviQI GTAC 2 cut(s) 492, 953
DdeI CTNAG 2 cut(s) 411, 426
DpnI GATC 3 cut(s) 639, 945, 959
DpnII GATC 3 cut(s) 637, 943, 957
EaeI YGGCCR 2 cut(s) 3, 175
Eam1104I CTCTTC 2 cut(s) 208, 278
EarI CTCTTC 2 cut(s) 208, 278
EciI GGCGGA 1 cut(s) 85
Eco130I CCWWGG 1 cut(s) 979
Eco31I GGTCTC 1 cut(s) 373
Eco57I CTGAAG 1 cut(s) 770
EcoO109I RGGNCCY 1 cut(s) 459
EcoRII CCWGG 1 cut(s) 438
EcoT14I CCWWGG 1 cut(s) 979
ErhI CCWWGG 1 cut(s) 979
FaeI CATG 4 cut(s) 265, 481, 617, 929
FaiI YATR 9 cut(s) 70, 188, 263, 354, 479, 525, 533, 615, 927
FalI AAGNNNNNCTT 2 cut(s) 368, 400
FatI CATG 4 cut(s) 261, 477, 613, 925
FauI CCCGC 1 cut(s) 89
FblI GTMKAC 1 cut(s) 897
Fnu4HI GCNGC 8 cut(s) 99, 102, 111, 136, 423, 777, 801, 969
FokI GGATG 5 cut(s) 154, 345, 596, 599, 731
Fsp4HI GCNGC 8 cut(s) 99, 102, 111, 136, 423, 777, 801, 969
FspBI CTAG 1 cut(s) 980
GlaI GCGC 1 cut(s) 221
GluI GCNGC 8 cut(s) 99, 102, 111, 136, 423, 777, 801, 969
GsuI CTGGAG 2 cut(s) 676, 677
HaeIII GGCC 5 cut(s) 5, 177, 317, 416, 460
HapII CCGG 1 cut(s) 584
HgaI GACGC 2 cut(s) 219, 367
HhaI GCGC 1 cut(s) 222
Hin1I GRCGYC 1 cut(s) 230
Hin1II CATG 4 cut(s) 265, 481, 617, 929
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HindIII AAGCTT 2 cut(s) 389, 482
HinfI GANTC 4 cut(s) 140, 367, 552, 659
HpaII CCGG 1 cut(s) 584
HphI GGTGA 4 cut(s) 49, 151, 253, 352
Hpy166II GTNNAC 2 cut(s) 688, 898
Hpy188I TCNGA 4 cut(s) 130, 172, 396, 664
Hpy188III TCNNGA 7 cut(s) 13, 262, 364, 466, 478, 560, 926
Hpy8I GTNNAC 2 cut(s) 688, 898
Hpy99I CGWCG 8 cut(s) 134, 176, 235, 238, 241, 310, 323, 326
HpyAV CCTTC 5 cut(s) 166, 172, 307, 472, 911
HpyCH4III ACNGT 1 cut(s) 593
HpyCH4V TGCA 3 cut(s) 497, 794, 847
HpyF10VI GCNNNNNNNGC 9 cut(s) 90, 107, 110, 183, 422, 494, 785, 800, 968
HpyF3I CTNAG 2 cut(s) 411, 426
Hsp92I GRCGYC 1 cut(s) 230
Hsp92II CATG 4 cut(s) 265, 481, 617, 929
HspAI GCGC 1 cut(s) 220
Kzo9I GATC 3 cut(s) 637, 943, 957
LmnI GCTCC 2 cut(s) 98, 797
Lsp1109I GCAGC 5 cut(s) 88, 409, 788, 812, 955
LweI GCATC 6 cut(s) 237, 333, 459, 618, 775, 971
MaeI CTAG 1 cut(s) 980
MaeIII GTNAC 2 cut(s) 241, 301
MalI GATC 3 cut(s) 639, 945, 959
MboI GATC 3 cut(s) 637, 943, 957
MboII GAAGA 8 cut(s) 135, 195, 198, 295, 763, 866, 893, 896
MfeI CAATTG 2 cut(s) 780, 816
MhlI GDGCHC 1 cut(s) 583
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 7, 86, 665, 770, 780, 816, 870
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 149, 361, 668
MmeI TCCRAC 3 cut(s) 153, 195, 419
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 3 cut(s) 287, 507, 888
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 386, 675, 1000
MslI CAYNNNNRTG 1 cut(s) 299
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 101, 968
MspI CCGG 1 cut(s) 584
MspR9I CCNGG 2 cut(s) 440, 584
MunI CAATTG 2 cut(s) 780, 816
MvaI CCWGG 1 cut(s) 440
MvnI CGCG 1 cut(s) 222
MwoI GCNNNNNNNGC 9 cut(s) 90, 107, 110, 183, 422, 494, 785, 800, 968
NciI CCSGG 1 cut(s) 584
NdeII GATC 3 cut(s) 637, 943, 957
NlaIII CATG 4 cut(s) 265, 481, 617, 929
NmeAIII GCCGAG 1 cut(s) 139
NmuCI GTSAC 2 cut(s) 241, 301
OliI CACNNNNGTG 1 cut(s) 299
PagI TCATGA 3 cut(s) 261, 477, 925
PcsI WCGNNNNNNNCGW 2 cut(s) 233, 242
PdmI GAANNNNTTC 3 cut(s) 287, 507, 888
PfeI GAWTC 1 cut(s) 552
PflFI GACNNNGTC 1 cut(s) 268
PkrI GCNGC 8 cut(s) 100, 103, 112, 137, 424, 778, 802, 970
PleI GAGTC 3 cut(s) 148, 361, 667
PpsI GAGTC 3 cut(s) 148, 361, 667
Psp6I CCWGG 1 cut(s) 438
PspGI CCWGG 1 cut(s) 438
PspPI GGNCC 1 cut(s) 459
PstI CTGCAG 1 cut(s) 849
PstNI CAGNNNCTG 1 cut(s) 971
PsyI GACNNNGTC 1 cut(s) 268
PvuII CAGCTG 1 cut(s) 968
RsaI GTAC 2 cut(s) 493, 954
RsaNI GTAC 2 cut(s) 492, 953
RseI CAYNNNNRTG 1 cut(s) 299
SaqAI TTAA 3 cut(s) 386, 675, 1000
SatI GCNGC 8 cut(s) 99, 102, 111, 136, 423, 777, 801, 969
Sau3AI GATC 3 cut(s) 637, 943, 957
Sau96I GGNCC 1 cut(s) 459
SchI GAGTC 3 cut(s) 149, 361, 668
ScrFI CCNGG 2 cut(s) 440, 584
SduI GDGCHC 1 cut(s) 583
SfaNI GCATC 6 cut(s) 237, 333, 459, 618, 775, 971
SfcI CTRYAG 2 cut(s) 735, 845
SmiMI CAYNNNNRTG 1 cut(s) 299
SmlI CTYRAG 2 cut(s) 297, 362
SmoI CTYRAG 2 cut(s) 297, 362
Sse9I AATT 7 cut(s) 7, 86, 665, 770, 780, 816, 870
SsiI CCGC 7 cut(s) 63, 82, 96, 99, 108, 111, 135
SspMI CTAG 1 cut(s) 980
StyD4I CCNGG 2 cut(s) 438, 582
StyI CCWWGG 1 cut(s) 979
TaaI ACNGT 1 cut(s) 593
TasI AATT 7 cut(s) 7, 86, 665, 770, 780, 816, 870
TatI WGTACW 2 cut(s) 491, 952
TauI GCSGC 3 cut(s) 101, 113, 138
TfiI GAWTC 1 cut(s) 552
Tru1I TTAA 3 cut(s) 386, 675, 1000
Tru9I TTAA 3 cut(s) 386, 675, 1000
TscAI CASTG 1 cut(s) 598
TseFI GTSAC 2 cut(s) 241, 301
TseI GCWGC 5 cut(s) 101, 422, 776, 800, 968
Tsp45I GTSAC 2 cut(s) 241, 301
TspDTI ATGAA 3 cut(s) 250, 494, 630
TspGWI ACGGA 1 cut(s) 199
TspRI CASTG 1 cut(s) 598
Tth111I GACNNNGTC 1 cut(s) 268
XapI RAATTY 1 cut(s) 665
XcmI CCANNNNNNNNNTGG 2 cut(s) 446, 648
XmaJI CCTAGG 1 cut(s) 979
XmiI GTMKAC 1 cut(s) 897
XmnI GAANNNNTTC 3 cut(s) 287, 507, 888
XspI CTAG 1 cut(s) 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.