Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0005183

Physical Location & Seq

Genomic Coordinates Forward (+)

2181385 .. 2183782

Gene Structure 2398 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1035700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1485 bp

ATGGCGAAACAAGTTTTTGTTTATGCTACAATCTTCATACTGATCACCACCGCTGGCTTTGCAGCTGCTATGCCCTCTGAGTCGAACTGCAGCCTTCATTTTGATTTGGGATCATCCCCGTCTCCAAATTCAACTTGCGAAGAAGGGGACTGGGGAGGGTTTCTGCACAAAGACTGCTGCGCATCAGCGTTCAATGACTACCTCTACGCGTTGGGGCAGCGCGCGAATCAAACAGGGTTGATATACCTCGACTCTGCCGAGCAGGGGGTTTGCTTGGCTTCCATGAAAAATTTGCAGAAGGATGTTTTCGGCTGTGGGATTGAGAAGTTGACAAGTGGTGCCGGTGGCTGCTCTGACTTGTCTGTCGCTGAAGTTAGGAATAGATTGGGCGACAAGTTGAAAGGTTTGGAGAGCCAGTGTGGATTCCGGTTTTCTGATGGAAAAAACGGGCAGCTGTGCGGTTCTTGTGTGAAGAGTTGGGAAGACATAGGACCATTGCATTCATCGTCCCGGGATGCAGAATCTTTGGAGTTTCAAACTAATGTTTGCAGGTTCGCAGTGCTGGTGTCGTATACAAGCATTAAACTTGAAGATAAAGGGCATCTTCTTGCAGTTTATAGTTGCCTTGGGTCACAAGAAGGCTTCGCTGGAAAGGTGTATAAAGGCATACTATCAAACAATCAACGTGTAGCAGTTAAGCACATTATCAATGACGGAAATGTGGAGACATTTGCCCGAGAAGTTACGAGCTTATCGCACATCAGACACCCGAACCTTGTTACCTTGCTAGGTTATTGTGCAAACGAAGATGAATGCTTCCTTGTCTATGAGCTTTGTCCCAACGGAAACCTCTCAGAATGGATTTTCGGGAAAGATAGGAACCTGTCCTGGATCCACAGGCTTGAGATTGCAATTGATAGCGCTCGAGGTCTATCGTTCCTCCACACGTATCCAGAAGGCTGCATTGTCCATCGCGACATCAAGCCAACAAACATTCTCCTCGGGGAGAATTTCGAAGCAAAGCTATCAGACTTTGGACTCTCCAAGATTATTGAGCTGGGAGAAACCTATGTCAGTTCAGAAGTGAGAGGAACTTTCGGGTACGTTGACCCAGACTATAGGATCAACAACCACGTAAATTCATCAGGAGATGTCTACAGCTTTGGAATCGTACTTCTGCAGATCCTATCGGGGAAGAAGGTTATCAACATGAATCTGAACACTCCAATGCCATTAAATAAACAGGCAAAGGTCCTCAACCGAGATGGCAGCATCAATGAATTCGCCGATCCTAAACTTGACGGGGAATACTCAGAAGAAGCGTTTGATCTAACGCTTCAGCTGGCTCTCTCATGCACAGCACTCAAACAACAAAGACCGTCAATGCAGCAAATTGTTGTGAGACTACAAGAGGCGCATGACATATCAACGCATGTAAAGGCCTCTACTCCTGAAGATTCACCAGATGCTAGCTTCTCGTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

495

Amino Acids

54.21

Weight (kDa)

5.3

Isoelectric Point (pI)

34.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPARK	PF19160	26 - 169	3.3e-11	SPARK
Pkinase	PF00069	213 - 466	5e-38	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	213 - 410	4.4e-37	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005183)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g27000 FvH4_4g34600
malus_domestica	MD09G1035700.v1.1 MD13G1090700.v1.1 MD13G1091000.v1.1
prunus_persica	Prupe.1G254900_v2.0.a1 Prupe.3G282300_v2.0.a1
pyrus_communis	pycom16g07810
rosa_chinensis	RchiOBHm_Chr2g0170941 RchiOBHm_Chr4g0443971
rosa_laevigata	RLG00000005871 RLG00000021994
rosa_multiflora	Rmu_sc0010716.1_g000005 Rmu_sc0025148.1_g000001 Rmu_sc0027702.1_g000001 Rmu_sc0029481.1_g000001
rosa_roxburghii	Rroxscaffold_2G00080960 Rroxscaffold_5G00384520
rosa_rugosa	Rorug02G0552500 Rorug04G0348600
rosa_samantha	Rh2AG625300 Rh2BG636100 Rh2CG604600 Rh2DG647700 Rh4AG409300 Rh4BG420400 Rh4CG434400 Rh4DG415400
rosa_wichuraiana	Rw2G051790 Rw4G035160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	362
Acc16I	TGCGCA	1 cut(s)	181
Acc36I	ACCTGC	1 cut(s)	540
AccB1I	GGYRCC	1 cut(s)	338
AccI	GTMKAC	2 cut(s)	572, 1155
AccII	CGCG	4 cut(s)	209, 222, 224, 975
AciI	CCGC	2 cut(s)	51, 459
AclWI	GGATC	6 cut(s)	118, 886, 899, 1130, 1177, 1283
AcsI	RAATTY	5 cut(s)	127, 289, 1009, 1138, 1280
AcuI	CTGAAG	3 cut(s)	390, 1322, 1473
AfaI	GTAC	2 cut(s)	1103, 1173
AfeI	AGCGCT	1 cut(s)	922
AfiI	CCNNNNNNNGG	1 cut(s)	264
AflIII	ACRYGT	3 cut(s)	207, 685, 945
AgsI	TTSAA	5 cut(s)	132, 193, 400, 536, 590
AjnI	CCWGG	1 cut(s)	887
AluBI	AGCT	9 cut(s)	65, 454, 750, 832, 1024, 1057, 1161, 1342, 1473
AluI	AGCT	9 cut(s)	65, 454, 750, 832, 1024, 1057, 1161, 1342, 1473
Alw26I	GTCTC	3 cut(s)	126, 719, 1396
AlwI	GGATC	6 cut(s)	118, 886, 899, 1130, 1177, 1283
Ama87I	CYCGRG	4 cut(s)	510, 735, 924, 1001
Aor51HI	AGCGCT	1 cut(s)	922
AoxI	GGCC	1 cut(s)	1440
ApoI	RAATTY	5 cut(s)	127, 289, 1009, 1138, 1280
AspLEI	GCGC	5 cut(s)	182, 222, 224, 923, 1417
AspS9I	GGNCC	2 cut(s)	491, 1252
AsuC2I	CCSGG	2 cut(s)	511, 512
AsuHPI	GGTGA	2 cut(s)	37, 1452
AsuII	TTCGAA	1 cut(s)	1014
AsuNHI	GCTAGC	1 cut(s)	1469
AvaI	CYCGRG	4 cut(s)	510, 735, 924, 1001
AvaII	GGWCC	2 cut(s)	491, 1252
BamHI	GGATCC	1 cut(s)	891
BanI	GGYRCC	1 cut(s)	338
BbsI	GAAGAC	1 cut(s)	489
BccI	CCATC	3 cut(s)	431, 978, 1259
BciT130I	CCWGG	1 cut(s)	889
BciVI	GTATCC	1 cut(s)	960
BclI	TGATCA	1 cut(s)	42
BcnI	CCSGG	2 cut(s)	511, 512
BcoDI	GTCTC	3 cut(s)	126, 719, 1396
BfaI	CTAG	2 cut(s)	788, 1470
BfmI	CTRYAG	4 cut(s)	88, 1117, 1156, 1178
BfoI	RGCGCY	1 cut(s)	924
BfuAI	ACCTGC	1 cut(s)	540
BfuI	GTATCC	1 cut(s)	960
Bme1390I	CCNGG	3 cut(s)	511, 512, 889
Bme18I	GGWCC	2 cut(s)	491, 1252
BmeT110I	CYCGRG	4 cut(s)	510, 735, 924, 1001
BmgT120I	GGNCC	2 cut(s)	491, 1252
BmiI	GGNNCC	3 cut(s)	340, 881, 893
BmrFI	CCNGG	3 cut(s)	511, 512, 889
BmrI	ACTGGG	1 cut(s)	160
BmsI	GCATC	5 cut(s)	191, 505, 610, 1281, 1456
BmtI	GCTAGC	1 cut(s)	1473
BmuI	ACTGGG	1 cut(s)	160
BpiI	GAAGAC	1 cut(s)	489
Bpu14I	TTCGAA	1 cut(s)	1014
BpuEI	CTTGAG	1 cut(s)	923
BpuMI	CCSGG	2 cut(s)	511, 512
BsaAI	YACGTR	2 cut(s)	948, 1135
BsaJI	CCNNGG	3 cut(s)	510, 625, 1000
BsaWI	WCCGGW	1 cut(s)	426
BsaXI	ACNNNNNCTCC	2 cut(s)	401, 431
Bsc4I	CCNNNNNNNGG	1 cut(s)	264
Bse118I	RCCGGY	1 cut(s)	341
Bse1I	ACTGG	2 cut(s)	155, 415
Bse3DI	GCAATG	1 cut(s)	494
BseBI	CCWGG	1 cut(s)	889
BseDI	CCNNGG	3 cut(s)	510, 625, 1000
BseGI	GGATG	3 cut(s)	113, 307, 520
BseLI	CCNNNNNNNGG	1 cut(s)	264
BseMI	GCAATG	1 cut(s)	494
BseMII	CTCAG	3 cut(s)	69, 867, 1326
BseNI	ACTGG	2 cut(s)	155, 415
BsePI	GCGCGC	1 cut(s)	220
BseRI	GAGGAG	1 cut(s)	989
BseYI	CCCAGC	1 cut(s)	1057
BsgI	GTGCAG	1 cut(s)	149
Bsh1236I	CGCG	4 cut(s)	209, 222, 224, 975
BshFI	GGCC	1 cut(s)	1442
BshNI	GGYRCC	1 cut(s)	338
BsiHKCI	CYCGRG	4 cut(s)	510, 735, 924, 1001
BsiSI	CCGG	3 cut(s)	342, 427, 511
BslFI	GGGAC	3 cut(s)	161, 493, 822
BslI	CCNNNNNNNGG	1 cut(s)	264
BsmAI	GTCTC	3 cut(s)	126, 719, 1396
BsmBI	CGTCTC	1 cut(s)	126
BsmFI	GGGAC	3 cut(s)	161, 493, 822
BsmI	GAATGC	2 cut(s)	499, 818
BsnI	GGCC	1 cut(s)	1442
BsoBI	CYCGRG	4 cut(s)	510, 735, 924, 1001
Bsp119I	TTCGAA	1 cut(s)	1014
Bsp143I	GATC	7 cut(s)	42, 110, 891, 1122, 1182, 1288, 1327
Bsp68I	TCGCGA	1 cut(s)	975
BspACI	CCGC	2 cut(s)	51, 459
BspANI	GGCC	1 cut(s)	1442
BspCNI	CTCAG	3 cut(s)	70, 866, 1325
BspFNI	CGCG	4 cut(s)	209, 222, 224, 975
BspLI	GGNNCC	3 cut(s)	340, 881, 893
BspMAI	CTGCAG	2 cut(s)	92, 1182
BspMI	ACCTGC	1 cut(s)	540
BspOI	GCTAGC	1 cut(s)	1473
BspPI	GGATC	6 cut(s)	118, 886, 899, 1130, 1177, 1283
BspT104I	TTCGAA	1 cut(s)	1014
BspT107I	GGYRCC	1 cut(s)	338
BsrDI	GCAATG	1 cut(s)	494
BsrFI	RCCGGY	1 cut(s)	341
BsrI	ACTGG	2 cut(s)	155, 415
BssAI	RCCGGY	1 cut(s)	341
BssECI	CCNNGG	3 cut(s)	510, 625, 1000
BssHII	GCGCGC	1 cut(s)	220
BssMI	GATC	7 cut(s)	42, 110, 891, 1122, 1182, 1288, 1327
BssNAI	GTATAC	1 cut(s)	573
BssT1I	CCWWGG	1 cut(s)	625
Bst1107I	GTATAC	1 cut(s)	573
Bst2UI	CCWGG	1 cut(s)	889
Bst4CI	ACNGT	1 cut(s)	1380
Bst6I	CTCTTC	1 cut(s)	467
BstBAI	YACGTR	2 cut(s)	948, 1135
BstBI	TTCGAA	1 cut(s)	1014
BstC8I	GCNNGC	4 cut(s)	55, 222, 1344, 1471
BstDEI	CTNAG	3 cut(s)	78, 853, 1312
BstF5I	GGATG	3 cut(s)	113, 307, 520
BstFNI	CGCG	4 cut(s)	209, 222, 224, 975
BstH2I	RGCGCY	1 cut(s)	924
BstHHI	GCGC	5 cut(s)	182, 222, 224, 923, 1417
BstKTI	GATC	7 cut(s)	45, 113, 894, 1125, 1185, 1291, 1330
BstMAI	GTCTC	3 cut(s)	126, 719, 1396
BstMBI	GATC	7 cut(s)	42, 110, 891, 1122, 1182, 1288, 1327
BstMWI	GCNNNNNNNGC	1 cut(s)	59
BstNI	CCWGG	1 cut(s)	889
BstNSI	RCATGY	1 cut(s)	1436
BstSCI	CCNGG	3 cut(s)	509, 510, 887
BstSFI	CTRYAG	4 cut(s)	88, 1117, 1156, 1178
BstUI	CGCG	4 cut(s)	209, 222, 224, 975
BstV2I	GAAGAC	1 cut(s)	489
BstX2I	RGATCY	2 cut(s)	891, 1182
BstYI	RGATCY	2 cut(s)	891, 1182
BstZ17I	GTATAC	1 cut(s)	573
BsuI	GTATCC	1 cut(s)	960
BsuRI	GGCC	1 cut(s)	1442
BtgZI	GCGATG	1 cut(s)	956
BtsCI	GGATG	3 cut(s)	113, 307, 520
BtsI	GCAGTG	1 cut(s)	564
BtsIMutI	CAGTG	2 cut(s)	422, 564
BtuMI	TCGCGA	1 cut(s)	975
BveI	ACCTGC	1 cut(s)	540
Cac8I	GCNNGC	4 cut(s)	55, 222, 1344, 1471
CfoI	GCGC	5 cut(s)	182, 222, 224, 923, 1417
Cfr10I	RCCGGY	1 cut(s)	341
Cfr13I	GGNCC	2 cut(s)	491, 1252
Cfr9I	CCCGGG	1 cut(s)	510
Csp6I	GTAC	2 cut(s)	1102, 1172
CviAII	CATG	5 cut(s)	283, 1210, 1353, 1418, 1433
CviQI	GTAC	2 cut(s)	1102, 1172
DdeI	CTNAG	3 cut(s)	78, 853, 1312
DpnI	GATC	7 cut(s)	44, 112, 893, 1124, 1184, 1290, 1329
DpnII	GATC	7 cut(s)	42, 110, 891, 1122, 1182, 1288, 1327
DrdI	GACNNNNNNGTC	1 cut(s)	362
DseDI	GACNNNNNNGTC	1 cut(s)	362
Eam1104I	CTCTTC	1 cut(s)	467
EarI	CTCTTC	1 cut(s)	467
Eco130I	CCWWGG	1 cut(s)	625
Eco147I	AGGCCT	1 cut(s)	1442
Eco47I	GGWCC	2 cut(s)	491, 1252
Eco47III	AGCGCT	1 cut(s)	922
Eco57I	CTGAAG	3 cut(s)	390, 1322, 1473
Eco88I	CYCGRG	4 cut(s)	510, 735, 924, 1001
EcoO109I	RGGNCCY	1 cut(s)	1252
EcoRI	GAATTC	1 cut(s)	1280
EcoRII	CCWGG	1 cut(s)	887
EcoT14I	CCWWGG	1 cut(s)	625
ErhI	CCWWGG	1 cut(s)	625
Esp3I	CGTCTC	1 cut(s)	126
FaeI	CATG	5 cut(s)	286, 1213, 1356, 1421, 1436
FalI	AAGNNNNNCTT	2 cut(s)	588, 620
FaqI	GGGAC	3 cut(s)	161, 493, 822
FatI	CATG	5 cut(s)	282, 1209, 1352, 1417, 1432
FbaI	TGATCA	1 cut(s)	42
FblI	GTMKAC	2 cut(s)	572, 1155
FokI	GGATG	3 cut(s)	100, 314, 527
FspBI	CTAG	2 cut(s)	788, 1470
FspI	TGCGCA	1 cut(s)	181
GlaI	GCGC	5 cut(s)	181, 221, 223, 922, 1416
GsaI	CCCAGC	1 cut(s)	1061
HaeII	RGCGCY	1 cut(s)	924
HaeIII	GGCC	1 cut(s)	1442
HapII	CCGG	3 cut(s)	342, 427, 511
HhaI	GCGC	5 cut(s)	182, 222, 224, 923, 1417
Hin1II	CATG	5 cut(s)	286, 1213, 1356, 1421, 1436
Hin6I	GCGC	5 cut(s)	180, 220, 222, 921, 1415
HinP1I	GCGC	5 cut(s)	180, 220, 222, 921, 1415
HincII	GTYRAC	2 cut(s)	330, 1108
HindII	GTYRAC	2 cut(s)	330, 1108
HinfI	GANTC	9 cut(s)	80, 226, 251, 423, 521, 1038, 1167, 1213, 1457
HpaII	CCGG	3 cut(s)	342, 427, 511
HphI	GGTGA	2 cut(s)	37, 1452
Hpy166II	GTNNAC	4 cut(s)	330, 573, 1108, 1156
Hpy188I	TCNGA	9 cut(s)	79, 355, 436, 764, 856, 1030, 1081, 1218, 1315
Hpy188III	TCNNGA	5 cut(s)	868, 953, 974, 1146, 1451
Hpy8I	GTNNAC	4 cut(s)	330, 573, 1108, 1156
HpyAV	CCTTC	6 cut(s)	104, 137, 292, 632, 950, 1192
HpyCH4III	ACNGT	1 cut(s)	1380
HpyCH4IV	ACGT	4 cut(s)	685, 947, 1104, 1134
HpyF10VI	GCNNNNNNNGC	1 cut(s)	59
HpyF3I	CTNAG	3 cut(s)	78, 853, 1312
HpySE526I	ACGT	4 cut(s)	685, 947, 1104, 1134
Hsp92II	CATG	5 cut(s)	286, 1213, 1356, 1421, 1436
HspAI	GCGC	5 cut(s)	180, 220, 222, 921, 1415
Ksp22I	TGATCA	1 cut(s)	42
Kzo9I	GATC	7 cut(s)	42, 110, 891, 1122, 1182, 1288, 1327
LweI	GCATC	5 cut(s)	191, 505, 610, 1281, 1456
MaeI	CTAG	2 cut(s)	788, 1470
MaeII	ACGT	4 cut(s)	685, 947, 1104, 1134
MaeIII	GTNAC	3 cut(s)	630, 742, 778
MalI	GATC	7 cut(s)	44, 112, 893, 1124, 1184, 1290, 1329
MboI	GATC	7 cut(s)	42, 110, 891, 1122, 1182, 1288, 1327
MfeI	CAATTG	1 cut(s)	912
MflI	RGATCY	2 cut(s)	891, 1182
MluCI	AATT	7 cut(s)	127, 289, 912, 1009, 1138, 1280, 1392
MluI	ACGCGT	1 cut(s)	207
MlyI	GAGTC	3 cut(s)	89, 245, 1032
MseI	TTAA	3 cut(s)	582, 696, 1235
MslI	CAYNNNNRTG	1 cut(s)	1226
MspA1I	CMGCKG	4 cut(s)	53, 65, 454, 1342
MspI	CCGG	3 cut(s)	342, 427, 511
MspR9I	CCNGG	3 cut(s)	511, 512, 889
MunI	CAATTG	1 cut(s)	912
Mva1269I	GAATGC	2 cut(s)	499, 818
MvaI	CCWGG	1 cut(s)	889
MvnI	CGCG	4 cut(s)	209, 222, 224, 975
MwoI	GCNNNNNNNGC	1 cut(s)	59
NciI	CCSGG	2 cut(s)	511, 512
NdeII	GATC	7 cut(s)	42, 110, 891, 1122, 1182, 1288, 1327
NheI	GCTAGC	1 cut(s)	1469
NlaIII	CATG	5 cut(s)	286, 1213, 1356, 1421, 1436
NlaIV	GGNNCC	3 cut(s)	340, 881, 893
NmeAIII	GCCGAG	1 cut(s)	283
NmuCI	GTSAC	1 cut(s)	630
NruI	TCGCGA	1 cut(s)	975
NsbI	TGCGCA	1 cut(s)	181
NspI	RCATGY	1 cut(s)	1436
NspV	TTCGAA	1 cut(s)	1014
PaeR7I	CTCGAG	1 cut(s)	924
PauI	GCGCGC	1 cut(s)	220
PceI	AGGCCT	1 cut(s)	1442
PcsI	WCGNNNNNNNCGW	1 cut(s)	255
PctI	GAATGC	2 cut(s)	499, 818
PfeI	GAWTC	6 cut(s)	226, 423, 521, 1167, 1213, 1457
PfoI	TCCNGGA	1 cut(s)	887
PleI	GAGTC	3 cut(s)	88, 245, 1032
PpsI	GAGTC	3 cut(s)	88, 245, 1032
Ppu21I	YACGTR	2 cut(s)	948, 1135
PpuMI	RGGWCCY	1 cut(s)	1252
Psp5II	RGGWCCY	1 cut(s)	1252
Psp6I	CCWGG	1 cut(s)	887
PspFI	CCCAGC	1 cut(s)	1057
PspGI	CCWGG	1 cut(s)	887
PspN4I	GGNNCC	3 cut(s)	340, 881, 893
PspPI	GGNCC	2 cut(s)	491, 1252
PspPPI	RGGWCCY	1 cut(s)	1252
PspXI	VCTCGAGB	1 cut(s)	924
PstI	CTGCAG	2 cut(s)	92, 1182
PsuI	RGATCY	2 cut(s)	891, 1182
PteI	GCGCGC	1 cut(s)	220
PvuII	CAGCTG	3 cut(s)	65, 454, 1342
RruI	TCGCGA	1 cut(s)	975
RsaI	GTAC	2 cut(s)	1103, 1173
RsaNI	GTAC	2 cut(s)	1102, 1172
RseI	CAYNNNNRTG	1 cut(s)	1226
SaqAI	TTAA	3 cut(s)	582, 696, 1235
Sau3AI	GATC	7 cut(s)	42, 110, 891, 1122, 1182, 1288, 1327
Sau96I	GGNCC	2 cut(s)	491, 1252
SchI	GAGTC	3 cut(s)	89, 245, 1032
ScrFI	CCNGG	3 cut(s)	511, 512, 889
SfaNI	GCATC	5 cut(s)	191, 505, 610, 1281, 1456
SfcI	CTRYAG	4 cut(s)	88, 1117, 1156, 1178
Sfr274I	CTCGAG	1 cut(s)	924
SfuI	TTCGAA	1 cut(s)	1014
SinI	GGWCC	2 cut(s)	491, 1252
SlaI	CTCGAG	1 cut(s)	924
SmaI	CCCGGG	1 cut(s)	512
SmiMI	CAYNNNNRTG	1 cut(s)	1226
SmlI	CTYRAG	2 cut(s)	902, 924
SmoI	CTYRAG	2 cut(s)	902, 924
Sse9I	AATT	7 cut(s)	127, 289, 912, 1009, 1138, 1280, 1392
SseBI	AGGCCT	1 cut(s)	1442
SsiI	CCGC	2 cut(s)	51, 459
SspMI	CTAG	2 cut(s)	788, 1470
StuI	AGGCCT	1 cut(s)	1442
StyD4I	CCNGG	3 cut(s)	509, 510, 887
StyI	CCWWGG	1 cut(s)	625
TaaI	ACNGT	1 cut(s)	1380
TaiI	ACGT	4 cut(s)	688, 950, 1107, 1137
TaqI	TCGA	4 cut(s)	83, 249, 925, 1014
TasI	AATT	7 cut(s)	127, 289, 912, 1009, 1138, 1280, 1392
TfiI	GAWTC	6 cut(s)	226, 423, 521, 1167, 1213, 1457
Tru1I	TTAA	3 cut(s)	582, 696, 1235
Tru9I	TTAA	3 cut(s)	582, 696, 1235
TscAI	CASTG	2 cut(s)	422, 564
TseFI	GTSAC	1 cut(s)	630
Tsp45I	GTSAC	1 cut(s)	630
TspDTI	ATGAA	8 cut(s)	25, 86, 299, 492, 825, 1131, 1226, 1293
TspGWI	ACGGA	2 cut(s)	729, 858
TspMI	CCCGGG	1 cut(s)	510
TspRI	CASTG	2 cut(s)	422, 564
VpaK11BI	GGWCC	2 cut(s)	491, 1252
XapI	RAATTY	5 cut(s)	127, 289, 1009, 1138, 1280
XceI	RCATGY	1 cut(s)	1436
XhoI	CTCGAG	1 cut(s)	924
XmaI	CCCGGG	1 cut(s)	510
XmiI	GTMKAC	2 cut(s)	572, 1155
XspI	CTAG	2 cut(s)	788, 1470

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.