Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0005183

Physical Location & Seq

Genomic Coordinates Reverse (-)

85682010 .. 85684560

Gene Structure 2551 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG636100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1761 bp

ATGACTGAGCTTCTAAAGCAATCTAGTATTGTCCTCATCTTCATACTGGTCACCACCATCAGCTTTGCAGTCCCTCAGCCTCCTCCGTCGAACTGTAGCCTTCATTTTGATGATGTTCCATCCAATTCAAGTTGTGGAAATGGAGACTGGGGAGGGTTTCTGCACAATAGCTGCTGTGCAACAATATTTGACGAGTACCTCTACGCATTGGGGCAGCGAGCAAACCAAACAGGAAACATATTTCTGGACTCTACCGAGCAGCGGAATTGCCTTGATGTCATGCAAAAATTTCAGAAGAATGTGTTTGGCTGTGGGTTTGAGCAGCTGACTAGTGGTGCAGGTGGCTGCTCTGACTTCTCTATTGGTGATGTTACTAGTAAGTTCGGAGACAAGTTGAGAAGTTTAGATGAGAAATGTAACATTCAGAGTTCTGATGGATGTAGTTCCTGTGCTAAGAGTTGGGGAAATATAAGAGCATTGCCCTCATGGTCCTCCGATCCACAGTCTATTAAGGCTGAAACACAAGTTTGCAGGTTTGCAGTTCTGGTGACATACTCAAGGACTAAAATCCAAGATAAAGACCATCTCCTTACAGTTTATAAGTGCCTTGAGGCACAAAAGTATGATGATCAAGCCGGGAAGGGTAGAAAAATAAGCACACGTTTATTGATCCTCATCGGAGGAGCAATAGGTATTATCATTTCAGTAGTAACAGCTGCATACATTTTTTCCAAGAAACGTACCAAAACAACTCAACCACCAGTTGTCGCCAAAAAGACAACTCCATCGCCCAAAAAAGAAGCAGCATTCAAGGATGTAATTTTTAAGGAGTCCGGCTGTCCAAAAATTCCGATCAAGGAAGTGTTATATGCTACAGACAATCTAAGCCCAGTACATTTCCTTGGTGAAGGAACTGCTGGAAAGGTTTATAGAGGCATACTGTCGAATAATCAGCCAGTAGCAATTAAGCACATTATCGATGATGAAAATGTGGAGTCTTTTGCCAGAGAATTTACAAGCTTATCACACATCAGACACCCCAACCTTGTTACTTTGCTTGGTTACTGTCTACAGGAAGATGAGTGTTTCCTTGTGTATGAGCTTTGTCCAAATGGGAACCTCTCAGAATGGCTTTTCGGTAAAGATAAGTGCCTGTCCTGGATCCAGAGGCTTGAGATTGCAATTGATAGCGCTCAAGGTCTATTGTTTCTCCACACGTATCCAGAAGGCTGCATTGTTCATCGTGACATCAAGCCAACAAACATTCTCCTTGGGGACAATTTTGAAGCCAAGCTATCGGACTTTGGACTATCTAAGATCATTGAACTTGGTGAAACCTATGTGAGTTCAGAAGTGAGAGGAACTTTTGGTTATGTTGATCCTGACTACCAGAGAAACCACCATGTAAATTCTTCTGGAGATGTCTACAGCTTTGGAATTGTACTTCTACAAATCCTATCAGGAAAGAGGGTTATCAATATGAATCTGAACACACCACTGCCATTAAATAAAATGGCAAAAGGCCTTAACAGAGCTGGCAGCATCAATGAATTTGCCGATCCTAAACTCGATGGGGAATACTCAGCAGAAGCTTTCAATCTCACACTTCAGCTGGCTCTGTCATGCACAGAACTTAGACAACAAAGACCATCCATGGAGCAAATTGTTGTCAGATTAGAACAGGCACATGACATATCAACACAGGCAAAGGCCTCTACTCCTGAAGTTTCAATAGACAGCTACCAAAGTTCAAATCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

586

Amino Acids

64.68

Weight (kDa)

6.1

Isoelectric Point (pI)

40.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPARK	PF19160	30 - 158	8.3e-13	SPARK
Pkinase	PF00069	299 - 556	2.1e-39	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	300 - 491	4.3e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005183)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g27000 FvH4_4g34600
malus_domestica	MD09G1035700.v1.1 MD13G1090700.v1.1 MD13G1091000.v1.1
prunus_persica	Prupe.1G254900_v2.0.a1 Prupe.3G282300_v2.0.a1
pyrus_communis	pycom16g07810
rosa_chinensis	RchiOBHm_Chr2g0170941 RchiOBHm_Chr4g0443971
rosa_laevigata	RLG00000005871 RLG00000021994
rosa_multiflora	Rmu_sc0010716.1_g000005 Rmu_sc0025148.1_g000001 Rmu_sc0027702.1_g000001 Rmu_sc0029481.1_g000001
rosa_roxburghii	Rroxscaffold_2G00080960 Rroxscaffold_5G00384520
rosa_rugosa	Rorug02G0552500 Rorug04G0348600
rosa_samantha	Rh2AG625300 Rh2BG636100 Rh2CG604600 Rh2DG647700 Rh4AG409300 Rh4BG420400 Rh4CG434400 Rh4DG415400
rosa_wichuraiana	Rw2G051790 Rw4G035160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	600
AarI	CACCTGC	1 cut(s)	329
Acc36I	ACCTGC	2 cut(s)	329, 522
AccI	GTMKAC	2 cut(s)	1069, 1425
AciI	CCGC	1 cut(s)	262
AclWI	GGATC	6 cut(s)	491, 664, 1156, 1169, 1373, 1553
AcsI	RAATTY	5 cut(s)	287, 846, 1010, 1408, 1550
AcuI	CTGAAG	2 cut(s)	1592, 1743
AfaI	GTAC	4 cut(s)	197, 742, 894, 1443
AfeI	AGCGCT	1 cut(s)	1192
AfiI	CCNNNNNNNGG	1 cut(s)	261
AflIII	ACRYGT	2 cut(s)	659, 1215
AgsI	TTSAA	7 cut(s)	129, 811, 1286, 1325, 1597, 1731, 1752
AhlI	ACTAGT	2 cut(s)	329, 374
AjnI	CCWGG	1 cut(s)	1157
Alw26I	GTCTC	2 cut(s)	138, 381
AlwI	GGATC	6 cut(s)	491, 664, 1156, 1169, 1373, 1553
Aor51HI	AGCGCT	1 cut(s)	1192
AoxI	GGCC	2 cut(s)	1522, 1710
ApeKI	GCWGC	9 cut(s)	171, 214, 259, 322, 345, 716, 803, 1230, 1539
ApoI	RAATTY	5 cut(s)	287, 846, 1010, 1408, 1550
AspLEI	GCGC	1 cut(s)	1193
AspS9I	GGNCC	1 cut(s)	489
AsuC2I	CCSGG	1 cut(s)	637
AsuHPI	GGTGA	5 cut(s)	43, 377, 559, 917, 1343
AvaII	GGWCC	1 cut(s)	489
BamHI	GGATCC	1 cut(s)	1161
BbvCI	CCTCAGC	1 cut(s)	75
BbvI	GCAGC	9 cut(s)	158, 226, 271, 332, 334, 703, 815, 1217, 1551
BccI	CCATC	7 cut(s)	65, 127, 428, 591, 793, 1565, 1657
BciT130I	CCWGG	1 cut(s)	1159
BciVI	GTATCC	1 cut(s)	1230
BclI	TGATCA	1 cut(s)	628
BcnI	CCSGG	1 cut(s)	637
BcoDI	GTCTC	2 cut(s)	138, 381
BcuI	ACTAGT	2 cut(s)	329, 374
BfaI	CTAG	3 cut(s)	24, 330, 375
BfmI	CTRYAG	4 cut(s)	94, 873, 1070, 1426
BfoI	RGCGCY	1 cut(s)	1194
BfuAI	ACCTGC	2 cut(s)	329, 522
BfuI	GTATCC	1 cut(s)	1230
BisI	GCNGC	9 cut(s)	172, 215, 260, 323, 346, 717, 804, 1231, 1540
BlsI	GCNGC	9 cut(s)	173, 216, 261, 324, 347, 718, 805, 1232, 1541
Bme1390I	CCNGG	2 cut(s)	637, 1159
Bme18I	GGWCC	1 cut(s)	489
BmgT120I	GGNCC	1 cut(s)	489
BmiI	GGNNCC	2 cut(s)	1118, 1163
BmrFI	CCNGG	2 cut(s)	637, 1159
BmrI	ACTGGG	2 cut(s)	157, 884
BmsI	GCATC	1 cut(s)	1551
BmuI	ACTGGG	2 cut(s)	157, 884
BpmI	CTGGAG	1 cut(s)	1437
Bpu10I	CCTNAGC	1 cut(s)	75
BpuEI	CTTGAG	4 cut(s)	541, 629, 1179, 1193
BpuMI	CCSGG	1 cut(s)	637
Bsa29I	ATCGAT	1 cut(s)	978
BsaAI	YACGTR	1 cut(s)	1218
BsaBI	GATNNNNATC	1 cut(s)	674
BsaJI	CCNNGG	3 cut(s)	901, 1270, 1653
Bsc4I	CCNNNNNNNGG	1 cut(s)	261
Bse1I	ACTGG	5 cut(s)	51, 152, 761, 890, 956
Bse3DI	GCAATG	1 cut(s)	476
Bse8I	GATNNNNATC	1 cut(s)	674
BseBI	CCWGG	1 cut(s)	1159
BseCI	ATCGAT	1 cut(s)	978
BseDI	CCNNGG	3 cut(s)	901, 1270, 1653
BseGI	GGATG	4 cut(s)	119, 443, 820, 1649
BseJI	GATNNNNATC	1 cut(s)	674
BseLI	CCNNNNNNNGG	1 cut(s)	261
BseMI	GCAATG	1 cut(s)	476
BseMII	CTCAG	3 cut(s)	89, 1137, 1596
BseNI	ACTGG	5 cut(s)	51, 152, 761, 890, 956
BseRI	GAGGAG	2 cut(s)	72, 696
BseXI	GCAGC	9 cut(s)	158, 226, 271, 332, 334, 703, 815, 1217, 1551
BsgI	GTGCAG	2 cut(s)	146, 357
BshFI	GGCC	2 cut(s)	1524, 1712
BshVI	ATCGAT	1 cut(s)	978
BsiSI	CCGG	2 cut(s)	636, 834
BslFI	GGGAC	2 cut(s)	56, 1289
BslI	CCNNNNNNNGG	1 cut(s)	261
BsmAI	GTCTC	2 cut(s)	138, 381
BsmFI	GGGAC	2 cut(s)	56, 1289
BsmI	GAATGC	1 cut(s)	806
BsnI	GGCC	2 cut(s)	1524, 1712
Bsp143I	GATC	8 cut(s)	496, 628, 669, 852, 1161, 1317, 1378, 1558
Bsp19I	CCATGG	1 cut(s)	1653
BspACI	CCGC	1 cut(s)	262
BspANI	GGCC	2 cut(s)	1524, 1712
BspCNI	CTCAG	3 cut(s)	88, 1136, 1595
BspDI	ATCGAT	1 cut(s)	978
BspLI	GGNNCC	2 cut(s)	1118, 1163
BspMI	ACCTGC	2 cut(s)	329, 522
BspPI	GGATC	6 cut(s)	491, 664, 1156, 1169, 1373, 1553
BsrDI	GCAATG	1 cut(s)	476
BsrI	ACTGG	5 cut(s)	51, 152, 761, 890, 956
BssECI	CCNNGG	3 cut(s)	901, 1270, 1653
BssMI	GATC	8 cut(s)	496, 628, 669, 852, 1161, 1317, 1378, 1558
BssT1I	CCWWGG	3 cut(s)	901, 1270, 1653
Bst2UI	CCWGG	1 cut(s)	1159
Bst4CI	ACNGT	5 cut(s)	95, 504, 595, 942, 1067
BstBAI	YACGTR	1 cut(s)	1218
BstC8I	GCNNGC	3 cut(s)	219, 1537, 1614
BstDEI	CTNAG	8 cut(s)	6, 75, 453, 884, 1123, 1314, 1582, 1634
BstDSI	CCRYGG	1 cut(s)	1653
BstEII	GGTNACC	1 cut(s)	49
BstF5I	GGATG	4 cut(s)	119, 443, 820, 1649
BstH2I	RGCGCY	1 cut(s)	1194
BstHHI	GCGC	1 cut(s)	1193
BstKTI	GATC	8 cut(s)	499, 631, 672, 855, 1164, 1320, 1381, 1561
BstMAI	GTCTC	2 cut(s)	138, 381
BstMBI	GATC	8 cut(s)	496, 628, 669, 852, 1161, 1317, 1378, 1558
BstMWI	GCNNNNNNNGC	1 cut(s)	16
BstNI	CCWGG	1 cut(s)	1159
BstPI	GGTNACC	1 cut(s)	49
BstSCI	CCNGG	2 cut(s)	635, 1157
BstSFI	CTRYAG	4 cut(s)	94, 873, 1070, 1426
BstV1I	GCAGC	9 cut(s)	158, 226, 271, 332, 334, 703, 815, 1217, 1551
BstX2I	RGATCY	1 cut(s)	1161
BstYI	RGATCY	1 cut(s)	1161
Bsu15I	ATCGAT	1 cut(s)	978
BsuI	GTATCC	1 cut(s)	1230
BsuRI	GGCC	2 cut(s)	1524, 1712
BsuTUI	ATCGAT	1 cut(s)	978
BtgI	CCRYGG	1 cut(s)	1653
BtgZI	GCGATG	1 cut(s)	771
BtsCI	GGATG	4 cut(s)	119, 443, 820, 1649
BtsI	GCAGTG	1 cut(s)	1496
BtsIMutI	CAGTG	1 cut(s)	1496
BveI	ACCTGC	2 cut(s)	329, 522
Cac8I	GCNNGC	3 cut(s)	219, 1537, 1614
CfoI	GCGC	1 cut(s)	1193
Cfr13I	GGNCC	1 cut(s)	489
ClaI	ATCGAT	1 cut(s)	978
Csp6I	GTAC	4 cut(s)	196, 741, 893, 1442
CviAII	CATG	7 cut(s)	280, 486, 1403, 1623, 1654, 1688, 1758
CviQI	GTAC	4 cut(s)	196, 741, 893, 1442
DdeI	CTNAG	8 cut(s)	6, 75, 453, 884, 1123, 1314, 1582, 1634
DpnI	GATC	8 cut(s)	498, 630, 671, 854, 1163, 1319, 1380, 1560
DpnII	GATC	8 cut(s)	496, 628, 669, 852, 1161, 1317, 1378, 1558
Eco130I	CCWWGG	3 cut(s)	901, 1270, 1653
Eco147I	AGGCCT	2 cut(s)	1524, 1712
Eco47I	GGWCC	1 cut(s)	489
Eco47III	AGCGCT	1 cut(s)	1192
Eco57I	CTGAAG	2 cut(s)	1592, 1743
Eco91I	GGTNACC	1 cut(s)	49
EcoO65I	GGTNACC	1 cut(s)	49
EcoRII	CCWGG	1 cut(s)	1157
EcoT14I	CCWWGG	3 cut(s)	901, 1270, 1653
ErhI	CCWWGG	3 cut(s)	901, 1270, 1653
FaeI	CATG	7 cut(s)	283, 489, 1406, 1626, 1657, 1691, 1761
FaqI	GGGAC	2 cut(s)	56, 1289
FatI	CATG	7 cut(s)	279, 485, 1402, 1622, 1653, 1687, 1757
FbaI	TGATCA	1 cut(s)	628
FblI	GTMKAC	2 cut(s)	1069, 1425
Fnu4HI	GCNGC	9 cut(s)	172, 215, 260, 323, 346, 717, 804, 1231, 1540
FokI	GGATG	4 cut(s)	106, 450, 827, 1636
Fsp4HI	GCNGC	9 cut(s)	172, 215, 260, 323, 346, 717, 804, 1231, 1540
FspBI	CTAG	3 cut(s)	24, 330, 375
GlaI	GCGC	1 cut(s)	1192
GluI	GCNGC	9 cut(s)	172, 215, 260, 323, 346, 717, 804, 1231, 1540
GsuI	CTGGAG	1 cut(s)	1437
HaeII	RGCGCY	1 cut(s)	1194
HaeIII	GGCC	2 cut(s)	1524, 1712
HapII	CCGG	2 cut(s)	636, 834
HhaI	GCGC	1 cut(s)	1193
Hin1II	CATG	7 cut(s)	283, 489, 1406, 1626, 1657, 1691, 1761
Hin6I	GCGC	1 cut(s)	1191
HinP1I	GCGC	1 cut(s)	1191
HindIII	AAGCTT	2 cut(s)	1018, 1590
HinfI	GANTC	4 cut(s)	248, 830, 995, 1483
HpaII	CCGG	2 cut(s)	636, 834
HphI	GGTGA	5 cut(s)	43, 377, 559, 917, 1343
Hpy166II	GTNNAC	2 cut(s)	1070, 1426
Hpy188III	TCNNGA	8 cut(s)	245, 1165, 1223, 1244, 1382, 1416, 1461, 1721
Hpy8I	GTNNAC	2 cut(s)	1070, 1426
Hpy99I	CGWCG	1 cut(s)	91
HpyAV	CCTTC	4 cut(s)	110, 634, 902, 1220
HpyCH4III	ACNGT	5 cut(s)	95, 504, 595, 942, 1067
HpyCH4IV	ACGT	3 cut(s)	661, 739, 1217
HpyF10VI	GCNNNNNNNGC	1 cut(s)	16
HpyF3I	CTNAG	8 cut(s)	6, 75, 453, 884, 1123, 1314, 1582, 1634
HpySE526I	ACGT	3 cut(s)	661, 739, 1217
Hsp92II	CATG	7 cut(s)	283, 489, 1406, 1626, 1657, 1691, 1761
HspAI	GCGC	1 cut(s)	1191
Ksp22I	TGATCA	1 cut(s)	628
Kzo9I	GATC	8 cut(s)	496, 628, 669, 852, 1161, 1317, 1378, 1558
LmnI	GCTCC	2 cut(s)	683, 1657
Lsp1109I	GCAGC	9 cut(s)	158, 226, 271, 332, 334, 703, 815, 1217, 1551
LweI	GCATC	1 cut(s)	1551
MaeI	CTAG	3 cut(s)	24, 330, 375
MaeII	ACGT	3 cut(s)	661, 739, 1217
MaeIII	GTNAC	8 cut(s)	49, 370, 416, 547, 709, 1048, 1061, 1244
MalI	GATC	8 cut(s)	498, 630, 671, 854, 1163, 1319, 1380, 1560
MboI	GATC	8 cut(s)	496, 628, 669, 852, 1161, 1317, 1378, 1558
MboII	GAAGA	4 cut(s)	31, 307, 1088, 1404
MfeI	CAATTG	1 cut(s)	1182
MflI	RGATCY	1 cut(s)	1161
MlyI	GAGTC	3 cut(s)	242, 839, 1004
MseI	TTAA	5 cut(s)	510, 825, 966, 1505, 1527
MslI	CAYNNNNRTG	1 cut(s)	108
MspA1I	CMGCKG	4 cut(s)	262, 325, 716, 1612
MspI	CCGG	2 cut(s)	636, 834
MspR9I	CCNGG	2 cut(s)	637, 1159
MunI	CAATTG	1 cut(s)	1182
Mva1269I	GAATGC	1 cut(s)	806
MvaI	CCWGG	1 cut(s)	1159
MwoI	GCNNNNNNNGC	1 cut(s)	16
NciI	CCSGG	1 cut(s)	637
NcoI	CCATGG	1 cut(s)	1653
NdeII	GATC	8 cut(s)	496, 628, 669, 852, 1161, 1317, 1378, 1558
NlaIII	CATG	7 cut(s)	283, 489, 1406, 1626, 1657, 1691, 1761
NlaIV	GGNNCC	2 cut(s)	1118, 1163
NmuCI	GTSAC	3 cut(s)	49, 547, 1244
PaqCI	CACCTGC	1 cut(s)	329
PceI	AGGCCT	2 cut(s)	1524, 1712
PctI	GAATGC	1 cut(s)	806
PfeI	GAWTC	1 cut(s)	1483
PfoI	TCCNGGA	1 cut(s)	1157
PkrI	GCNGC	9 cut(s)	173, 216, 261, 324, 347, 718, 805, 1232, 1541
PleI	GAGTC	3 cut(s)	242, 838, 1003
PpsI	GAGTC	3 cut(s)	242, 838, 1003
Ppu21I	YACGTR	1 cut(s)	1218
PsiI	TTATAA	1 cut(s)	600
Psp6I	CCWGG	1 cut(s)	1157
PspEI	GGTNACC	1 cut(s)	49
PspGI	CCWGG	1 cut(s)	1157
PspN4I	GGNNCC	2 cut(s)	1118, 1163
PspPI	GGNCC	1 cut(s)	489
PsuI	RGATCY	1 cut(s)	1161
PvuII	CAGCTG	3 cut(s)	325, 716, 1612
RsaI	GTAC	4 cut(s)	197, 742, 894, 1443
RsaNI	GTAC	4 cut(s)	196, 741, 893, 1442
RseI	CAYNNNNRTG	1 cut(s)	108
SaqAI	TTAA	5 cut(s)	510, 825, 966, 1505, 1527
SatI	GCNGC	9 cut(s)	172, 215, 260, 323, 346, 717, 804, 1231, 1540
Sau3AI	GATC	8 cut(s)	496, 628, 669, 852, 1161, 1317, 1378, 1558
Sau96I	GGNCC	1 cut(s)	489
SchI	GAGTC	3 cut(s)	242, 839, 1004
ScrFI	CCNGG	2 cut(s)	637, 1159
SfaNI	GCATC	1 cut(s)	1551
SfcI	CTRYAG	4 cut(s)	94, 873, 1070, 1426
SinI	GGWCC	1 cut(s)	489
SmiMI	CAYNNNNRTG	1 cut(s)	108
SmlI	CTYRAG	4 cut(s)	556, 608, 1172, 1194
SmoI	CTYRAG	4 cut(s)	556, 608, 1172, 1194
SpeI	ACTAGT	2 cut(s)	329, 374
SseBI	AGGCCT	2 cut(s)	1524, 1712
SsiI	CCGC	1 cut(s)	262
SspI	AATATT	1 cut(s)	186
SspMI	CTAG	3 cut(s)	24, 330, 375
StuI	AGGCCT	2 cut(s)	1524, 1712
StyD4I	CCNGG	2 cut(s)	635, 1157
StyI	CCWWGG	3 cut(s)	901, 1270, 1653
TaaI	ACNGT	5 cut(s)	95, 504, 595, 942, 1067
TaiI	ACGT	3 cut(s)	664, 742, 1220
TaqI	TCGA	4 cut(s)	89, 944, 978, 1569
TatI	WGTACW	2 cut(s)	892, 1441
TfiI	GAWTC	1 cut(s)	1483
Tru1I	TTAA	5 cut(s)	510, 825, 966, 1505, 1527
Tru9I	TTAA	5 cut(s)	510, 825, 966, 1505, 1527
TscAI	CASTG	1 cut(s)	1503
TseFI	GTSAC	3 cut(s)	49, 547, 1244
TseI	GCWGC	9 cut(s)	171, 214, 259, 322, 345, 716, 803, 1230, 1539
Tsp45I	GTSAC	3 cut(s)	49, 547, 1244
TspDTI	ATGAA	6 cut(s)	31, 92, 999, 1229, 1496, 1563
TspGWI	ACGGA	1 cut(s)	75
TspRI	CASTG	1 cut(s)	1503
VpaK11BI	GGWCC	1 cut(s)	489
XapI	RAATTY	5 cut(s)	287, 846, 1010, 1408, 1550
XmiI	GTMKAC	2 cut(s)	1069, 1425
XspI	CTAG	3 cut(s)	24, 330, 375

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.