Rorug04G0348600

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005183
Physical Location & Seq
Reverse (-)
52085595 .. 52086920
1326 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0348600.1

Sequence Viewer

Length: 654 bp
ATGGGACAGAGCACTAATCATCAAATAATGGAGAGGCTATTCATGATCATAGTAATATGCTACCTCTGTGGTAGTATAGTTGAACTCTCTAGTCAAGCTGTCGAGTCGCCTCAGTACAGTGTGGTGCATTCAGAATCAGACTTTGAGGTCAGACTCTACAGAGAATCCTCATGGATGTCAACTCAAGTCCGAGAAACAACTTCATTCGAAAAAGCCACTAAAGATGGCTTTCACAGGTTGTATCAGTACATTCATGGTGATAACCTTAACTCGTCCCAGATTACCATGACAGCTCCGGTGTTAACAAGCATTGTCCCATCTGTGCATGGACCGGAATACTACGTAAGGTTGTATCTGCCTGCCAAATATGAAAGAACACCACCACAGCCTTTCACTGAACTGAATTTGCAGTTTGATAAGCGGAGAAGTCACTGCATAGCAACCAGAAAGTTTCCAGGGTTTGCCAAGGATGATGACATTAATGAGGAATTTGAAACTCTTGTGAGTAGCCTAAATAAGCACTTGAATGGAAAACCAGGAATCCTGAAGGAAAAGAGTTCTTATACAATAGCCCAGTATAATGCTTCATATCATGTTTCTGGGCGCTTAAATGAAGTGCGGATGGATCTCTCAGGATTTACTGCCGACTGCTGA

Protein Analysis

217

Amino Acids

24.85

Weight (kDa)

6.24

Isoelectric Point (pI)

42.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 34 - 208 5.3e-47 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 146
AciI CCGC 2 cut(s) 421, 619
AclWI GGATC 1 cut(s) 633
AcsI RAATTY 2 cut(s) 403, 488
AcuI CTGAAG 1 cut(s) 566
AfaI GTAC 2 cut(s) 116, 248
AgsI TTSAA 3 cut(s) 83, 494, 526
AjnI CCWGG 2 cut(s) 454, 535
AluBI AGCT 2 cut(s) 98, 293
AluI AGCT 2 cut(s) 98, 293
Alw21I GWGCWC 1 cut(s) 14
AlwI GGATC 1 cut(s) 633
ApoI RAATTY 2 cut(s) 403, 488
AseI ATTAAT 1 cut(s) 480
AspLEI GCGC 1 cut(s) 606
AspS9I GGNCC 1 cut(s) 329
AsuHPI GGTGA 1 cut(s) 269
AsuII TTCGAA 1 cut(s) 207
AvaII GGWCC 1 cut(s) 329
Bbv12I GWGCWC 1 cut(s) 14
BccI CCATC 3 cut(s) 218, 325, 616
BciT130I CCWGG 2 cut(s) 456, 537
BclI TGATCA 1 cut(s) 45
BfaI CTAG 1 cut(s) 90
BfmI CTRYAG 1 cut(s) 157
BfoI RGCGCY 1 cut(s) 607
Bme1390I CCNGG 2 cut(s) 456, 537
Bme18I GGWCC 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 329
BmrFI CCNGG 2 cut(s) 456, 537
BmrI ACTGGG 1 cut(s) 568
BmuI ACTGGG 1 cut(s) 568
Bpu14I TTCGAA 1 cut(s) 207
BpuEI CTTGAG 1 cut(s) 168
BsaAI YACGTR 1 cut(s) 343
BsaJI CCNNGG 2 cut(s) 455, 465
BsaWI WCCGGW 2 cut(s) 295, 331
Bse1I ACTGG 1 cut(s) 574
BseBI CCWGG 2 cut(s) 456, 537
BseDI CCNNGG 2 cut(s) 455, 465
BseGI GGATG 3 cut(s) 180, 475, 627
BseMII CTCAG 2 cut(s) 125, 645
BseNI ACTGG 1 cut(s) 574
BsiHKAI GWGCWC 1 cut(s) 14
BsiSI CCGG 2 cut(s) 296, 332
BslFI GGGAC 3 cut(s) 18, 259, 299
BsmFI GGGAC 3 cut(s) 18, 259, 299
BsmI GAATGC 1 cut(s) 127
Bsp119I TTCGAA 1 cut(s) 207
Bsp1286I GDGCHC 1 cut(s) 14
Bsp143I GATC 2 cut(s) 45, 625
BspACI CCGC 2 cut(s) 421, 619
BspCNI CTCAG 2 cut(s) 124, 644
BspHI TCATGA 1 cut(s) 42
BspPI GGATC 1 cut(s) 633
BspT104I TTCGAA 1 cut(s) 207
BsrI ACTGG 1 cut(s) 574
BssECI CCNNGG 2 cut(s) 455, 465
BssMI GATC 2 cut(s) 45, 625
BssT1I CCWWGG 1 cut(s) 465
Bst2UI CCWGG 2 cut(s) 456, 537
Bst4CI ACNGT 1 cut(s) 119
BstBAI YACGTR 1 cut(s) 343
BstBI TTCGAA 1 cut(s) 207
BstC8I GCNNGC 1 cut(s) 360
BstDEI CTNAG 2 cut(s) 111, 631
BstF5I GGATG 3 cut(s) 180, 475, 627
BstH2I RGCGCY 1 cut(s) 607
BstHHI GCGC 1 cut(s) 606
BstKTI GATC 2 cut(s) 48, 628
BstMBI GATC 2 cut(s) 45, 625
BstNI CCWGG 2 cut(s) 456, 537
BstSCI CCNGG 2 cut(s) 454, 535
BstSFI CTRYAG 1 cut(s) 157
BstSNI TACGTA 1 cut(s) 343
BstX2I RGATCY 1 cut(s) 625
BstYI RGATCY 1 cut(s) 625
BtsCI GGATG 3 cut(s) 180, 475, 627
BtsI GCAGTG 1 cut(s) 430
BtsIMutI CAGTG 3 cut(s) 124, 393, 430
Cac8I GCNNGC 1 cut(s) 360
CciI TCATGA 1 cut(s) 42
CfoI GCGC 1 cut(s) 606
Cfr13I GGNCC 1 cut(s) 329
Csp6I GTAC 2 cut(s) 115, 247
CviAII CATG 6 cut(s) 43, 171, 254, 286, 326, 593
CviJI RGCY 8 cut(s) 37, 98, 215, 228, 293, 388, 510, 572
CviKI_1 RGCY 8 cut(s) 37, 98, 215, 228, 293, 388, 510, 572
CviQI GTAC 2 cut(s) 115, 247
DdeI CTNAG 2 cut(s) 111, 631
DpnI GATC 2 cut(s) 47, 627
DpnII GATC 2 cut(s) 45, 625
DrdI GACNNNNNNGTC 1 cut(s) 146
DseDI GACNNNNNNGTC 1 cut(s) 146
Eco105I TACGTA 1 cut(s) 343
Eco130I CCWWGG 1 cut(s) 465
Eco47I GGWCC 1 cut(s) 329
Eco57I CTGAAG 1 cut(s) 566
EcoRII CCWGG 2 cut(s) 454, 535
EcoT14I CCWWGG 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 6 cut(s) 46, 174, 257, 289, 329, 596
FaqI GGGAC 3 cut(s) 18, 259, 299
FatI CATG 6 cut(s) 42, 170, 253, 285, 325, 592
FbaI TGATCA 1 cut(s) 45
FokI GGATG 3 cut(s) 187, 482, 634
FspBI CTAG 1 cut(s) 90
GlaI GCGC 1 cut(s) 605
HaeII RGCGCY 1 cut(s) 607
HapII CCGG 2 cut(s) 296, 332
HhaI GCGC 1 cut(s) 606
Hin1II CATG 6 cut(s) 46, 174, 257, 289, 329, 596
Hin6I GCGC 1 cut(s) 604
HinP1I GCGC 1 cut(s) 604
HincII GTYRAC 2 cut(s) 180, 303
HindII GTYRAC 2 cut(s) 180, 303
HinfI GANTC 5 cut(s) 104, 134, 153, 164, 540
HpaI GTTAAC 1 cut(s) 303
HpaII CCGG 2 cut(s) 296, 332
HphI GGTGA 1 cut(s) 269
Hpy166II GTNNAC 2 cut(s) 180, 303
Hpy188I TCNGA 4 cut(s) 133, 139, 152, 191
Hpy188III TCNNGA 3 cut(s) 43, 544, 633
Hpy8I GTNNAC 2 cut(s) 180, 303
HpyAV CCTTC 1 cut(s) 541
HpyCH4III ACNGT 1 cut(s) 119
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 4 cut(s) 127, 325, 409, 435
HpyF3I CTNAG 2 cut(s) 111, 631
HpySE526I ACGT 1 cut(s) 342
Hsp92II CATG 6 cut(s) 46, 174, 257, 289, 329, 596
HspAI GCGC 1 cut(s) 604
Ksp22I TGATCA 1 cut(s) 45
KspAI GTTAAC 1 cut(s) 303
Kzo9I GATC 2 cut(s) 45, 625
LmnI GCTCC 1 cut(s) 298
MaeI CTAG 1 cut(s) 90
MaeII ACGT 1 cut(s) 342
MaeIII GTNAC 1 cut(s) 428
MalI GATC 2 cut(s) 47, 627
MboI GATC 2 cut(s) 45, 625
MflI RGATCY 1 cut(s) 625
MhlI GDGCHC 1 cut(s) 14
MluCI AATT 2 cut(s) 403, 488
MlyI GAGTC 2 cut(s) 113, 147
MnlI CCTC 6 cut(s) 27, 74, 120, 139, 178, 478
MseI TTAA 4 cut(s) 267, 302, 480, 608
MslI CAYNNNNRTG 1 cut(s) 525
MspI CCGG 2 cut(s) 296, 332
MspR9I CCNGG 2 cut(s) 456, 537
Mva1269I GAATGC 1 cut(s) 127
MvaI CCWGG 2 cut(s) 456, 537
NdeII GATC 2 cut(s) 45, 625
NlaIII CATG 6 cut(s) 46, 174, 257, 289, 329, 596
NmuCI GTSAC 1 cut(s) 428
NspV TTCGAA 1 cut(s) 207
PagI TCATGA 1 cut(s) 42
PctI GAATGC 1 cut(s) 127
PfeI GAWTC 3 cut(s) 134, 164, 540
PleI GAGTC 2 cut(s) 112, 147
PpsI GAGTC 2 cut(s) 112, 147
Ppu21I YACGTR 1 cut(s) 343
PshBI ATTAAT 1 cut(s) 480
Psp6I CCWGG 2 cut(s) 454, 535
PspGI CCWGG 2 cut(s) 454, 535
PspPI GGNCC 1 cut(s) 329
PsuI RGATCY 1 cut(s) 625
RsaI GTAC 2 cut(s) 116, 248
RsaNI GTAC 2 cut(s) 115, 247
RseI CAYNNNNRTG 1 cut(s) 525
SaqAI TTAA 4 cut(s) 267, 302, 480, 608
Sau3AI GATC 2 cut(s) 45, 625
Sau96I GGNCC 1 cut(s) 329
SchI GAGTC 2 cut(s) 113, 147
ScrFI CCNGG 2 cut(s) 456, 537
SduI GDGCHC 1 cut(s) 14
SetI ASST 8 cut(s) 66, 100, 150, 239, 267, 295, 345, 350
SfcI CTRYAG 1 cut(s) 157
SfuI TTCGAA 1 cut(s) 207
SinI GGWCC 1 cut(s) 329
SmiMI CAYNNNNRTG 1 cut(s) 525
SmlI CTYRAG 1 cut(s) 183
SmoI CTYRAG 1 cut(s) 183
SnaBI TACGTA 1 cut(s) 343
Sse9I AATT 2 cut(s) 403, 488
SsiI CCGC 2 cut(s) 421, 619
SspMI CTAG 1 cut(s) 90
StyD4I CCNGG 2 cut(s) 454, 535
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 1 cut(s) 119
TaiI ACGT 1 cut(s) 345
TaqI TCGA 2 cut(s) 102, 207
TasI AATT 2 cut(s) 403, 488
TatI WGTACW 2 cut(s) 114, 246
TfiI GAWTC 3 cut(s) 134, 164, 540
Tru1I TTAA 4 cut(s) 267, 302, 480, 608
Tru9I TTAA 4 cut(s) 267, 302, 480, 608
TscAI CASTG 3 cut(s) 124, 400, 437
TseFI GTSAC 1 cut(s) 428
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 6 cut(s) 31, 192, 242, 384, 576, 627
TspRI CASTG 3 cut(s) 124, 400, 437
VpaK11BI GGWCC 1 cut(s) 329
VspI ATTAAT 1 cut(s) 480
XapI RAATTY 2 cut(s) 403, 488
XspI CTAG 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.