Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001667

Physical Location & Seq

Genomic Coordinates Reverse (-)

11380680 .. 11384778

Gene Structure 4099 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1146000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1656 bp

ATGAATTCTTGCATTCCCGAGTGGAACTTTGAGTCTGATCTCCCCTTGACCAACCAGAAGAAACCAATAGGGCCAGACCATGAACTTGTAGAGCTCTTGTGGAGAAATGGGCAAGTAGTTTTGAACAGCCAAACGCATCGAAAACCAAGTTTTAATCCGAATGATCAATCGAGACAAGTTCAGAAACATGATCAGCAATCAATAAGGTCTGGGGGGTTGTATGGGAATTCAAGTAATCTGATTCAAGATGATGACACCGTTTCACTTATCCACTACCCGCTTGAGGATTCTTTCGACAAAGAGTTTTGTTCCCATTTTTTCTCTGAATTGCCTTCCTGTGATCCAATTGAGCTTGACAAACCAATCAAACAGTTTGAGGGAGAGAAGGTTGTGAAGTTTGATGCTTCTGACACCGCGCGTCTTGTTTCCAACTCACCGCGGCCTAATGGCAAGTCCTCCGCTGGTGTGGAATACCCTGCAAATCCAATGCCTCCTCCAAGATATCAATACATTAATTCAACTGATCAGCAGAACCAAAATCAAGGAGGCTTGGGGAAAATAGTTAATTTTTCGCAATTTGCAACCCCGGGAAAGGTTTCAGCAGGATCTTCTAGAGGACAGTTAGGAGGGAAAGAGTCCGGAAATTTAGCGCAAGCGGAGGTTAGGGAGTGTTCTGTGATGACGGTTGGTTCGAGTTACGTTGGTAGCAACCAAGTTCTAAATGACCTTGATGTGAGCAGGGCTTCAAGCAATTGTGATGGAACTACTGGTTTATCGGCTGGACATTTCTACGACAACGTTCATAAGATAATGCCTCAGAGCGAGACAGGGAAAACGGATACGCTTGATCCAACGCTTACTTCGTCTTCTGGCGGTTCTGGTAGTAGTTTTGGCAGAGGAGGAAACCAGTCTAATGTTGTCAATAGCAACAAGAGAAAGGGTAGAGAGGCAGAAGACTCGGAGTGCCAAAGTGAGGCTGCTGAACTTGAATCAGCTTCAGCTGCGAGAAGTAAGTCAGCGCATCGATCAGGATCGACCAGGAGGAGTCGTGCTGCTGAAGTCCATAACCTCTCAGAAAGGAAACGGAGAGATCGGATTAACGAGAAAATGAGAGCATTGCAAGAGCTTATACCTCATTCTAACAAGACAGATAAAGCATCAATGTTAGATGAGGCAATTGATTACTTGAAGTCACTTCAGATGCAACTGCAGGTAATGTGGATGGGAAGCGGGATGGCACCAATGATGTTTCCGGGCATGCAGCACTATATTTCCCGAATGGGAATGGGAATGGGAATGGGAATGGGACCGCCGGCCTTGCCTTCTATGCACAATCCGATGCATTTACCTAGGCATCCGCTCGTTGATCAGTGCATGAATATGGCTCCAGCAGCAAACCAGACTGTAATGTGCCAACCACCGGTCTTGAATCCCATTGATTACCACAACCAGATGCAAAATCCCTCTTTTCAGGAACAATACATGCGTCTCATGAACTTCCATCACATGCAGACTATGTCTCAGCCAATGAATATGTTCAGATTTGGCTCCCAACCTCTACAACAGAATCAGATGATGGCACCAAATGGTCTCAGCAGTGGACCTTTCGGTGGAAGAGCTGCAACTAATGACCCTTTAAGTGGAAAAATGAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0007602 GO:0008150 GO:0008152 GO:0009058 GO:0009314 GO:0009416 GO:0009581 GO:0009582 GO:0009583 GO:0009585 GO:0009605 GO:0009628 GO:0009637 GO:0009639 GO:0009640 GO:0009642 GO:0009645 GO:0009692 GO:0009693 GO:0009704 GO:0009791 GO:0009889 GO:0009966 GO:0009987 GO:0010017 GO:0010114 GO:0010161 GO:0010244 GO:0010468 GO:0010556 GO:0010600 GO:0010646 GO:0010817 GO:0010928 GO:0019219 GO:0019222 GO:0023051 GO:0023052 GO:0031323 GO:0031326 GO:0032350 GO:0032501 GO:0032502 GO:0042445 GO:0042446 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043449 GO:0043450 GO:0044237 GO:0044249 GO:0044424 GO:0044464 GO:0046885 GO:0048583 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051606 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0071214 GO:0071478 GO:0071482 GO:0071489 GO:0071491 GO:0071704 GO:0080090 GO:0090354 GO:0097159 GO:0104004 GO:0140110 GO:1900673 GO:1900674 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

HLH

Protein Analysis

552

Amino Acids

60.67

Weight (kDa)

6.63

Isoelectric Point (pI)

54.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HLH	PF00010	353 - 400	6.2e-14	Helix-loop-helix DNA-binding domain
bHLH_SAC51	PF23173	359 - 405	3.1e-09	Transcription factor SAC51 bHLH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001667)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43010 AT2G43010 AT2G43010 AT2G43010 AT2G43010 AT3G59060 AT3G59060 AT3G59060 AT3G59060
fragaria_vesca	FvH4_6g38940 FvH4_6g38940 FvH4_6g38940 FvH4_6g38940 FvH4_6g38940 FvH4_6g38940 FvH4_6g38940 FvH4_6g38940 FvH4_6g38940 FvH4_6g38940 FvH4_6g38940 FvH4_6g38940 FvH4_6g38940
malus_domestica	MD09G1146000.v1.1 MD17G1132600.v1.1
prunus_persica	Prupe.3G179800_v2.0.a1 Prupe.3G179800_v2.0.a1 Prupe.3G179800_v2.0.a1 Prupe.3G179800_v2.0.a1 Prupe.3G179800_v2.0.a1 Prupe.3G179800_v2.0.a1 Prupe.3G179800_v2.0.a1
pyrus_communis	pycom09g06630 pycom17g12610
rosa_chinensis	RchiOBHm_Chr2g0152511
rosa_laevigata	RLG00000020642
rosa_multiflora	Rmu_sc0005366.1_g000001 Rmu_sc0027733.1_g000001 Rmu_sc0027737.1_g000001 Rmu_sc0034754.1_g000001
rosa_roxburghii	Rroxscaffold_2G00095640
rosa_rugosa	Rorug02G0434000 Rorug02G0434100 Rorug02G0434200
rosa_samantha	Rh2AG495400 Rh2BG507300 Rh2CG481500 Rh2CG481600 Rh2DG518600
rosa_wichuraiana	Rw2G040760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1201
AccB1I	GGYRCC	2 cut(s)	1237, 1579
AccBSI	CCGCTC	1 cut(s)	1360
AccII	CGCG	3 cut(s)	416, 418, 439
AccIII	TCCGGA	1 cut(s)	638
AclI	AACGTT	1 cut(s)	798
AclWI	GGATC	4 cut(s)	335, 613, 842, 1039
AcsI	RAATTY	3 cut(s)	4, 226, 643
AcuI	CTGAAG	3 cut(s)	981, 1077, 1181
AfiI	CCNNNNNNNGG	6 cut(s)	283, 592, 973, 1420, 1610, 1640
AgeI	ACCGGT	1 cut(s)	1420
AgsI	TTSAA	8 cut(s)	124, 231, 245, 519, 747, 989, 1189, 1429
AjnI	CCWGG	1 cut(s)	1037
AjuI	GAANNNNNNNTTGG	2 cut(s)	844, 876
AluBI	AGCT	6 cut(s)	94, 352, 995, 1001, 1126, 1619
AluI	AGCT	6 cut(s)	94, 352, 995, 1001, 1126, 1619
Alw21I	GWGCWC	1 cut(s)	96
Alw26I	GTCTC	5 cut(s)	166, 818, 1493, 1524, 1595
AlwI	GGATC	4 cut(s)	335, 613, 842, 1039
Ama87I	CYCGRG	2 cut(s)	17, 586
Aor13HI	TCCGGA	1 cut(s)	638
AoxI	GGCC	3 cut(s)	71, 440, 1314
ApeKI	GCWGC	6 cut(s)	977, 1001, 1052, 1261, 1391, 1619
ApoI	RAATTY	3 cut(s)	4, 226, 643
ArsI	GACNNNNNNTTYG	2 cut(s)	673, 705
AseI	ATTAAT	1 cut(s)	513
AsiGI	ACCGGT	1 cut(s)	1420
Asp700I	GAANNNNTTC	2 cut(s)	595, 1535
AspA2I	CCTAGG	1 cut(s)	1349
AspLEI	GCGC	3 cut(s)	418, 652, 1021
AspS9I	GGNCC	3 cut(s)	71, 1307, 1601
AsuC2I	CCSGG	3 cut(s)	587, 588, 1254
AsuHPI	GGTGA	1 cut(s)	426
AvaI	CYCGRG	2 cut(s)	17, 586
AvaII	GGWCC	2 cut(s)	1307, 1601
AvrII	CCTAGG	1 cut(s)	1349
BanI	GGYRCC	2 cut(s)	1237, 1579
BanII	GRGCYC	1 cut(s)	96
BbsI	GAAGAC	2 cut(s)	858, 960
Bbv12I	GWGCWC	1 cut(s)	96
BbvI	GCAGC	6 cut(s)	964, 988, 1039, 1273, 1403, 1606
BccI	CCATC	5 cut(s)	752, 1216, 1228, 1509, 1570
BcgI	CGANNNNNNTGC	2 cut(s)	939, 973
BciT130I	CCWGG	1 cut(s)	1039
BciVI	GTATCC	1 cut(s)	832
BclI	TGATCA	4 cut(s)	163, 190, 523, 1366
BcnI	CCSGG	3 cut(s)	587, 588, 1254
BcoDI	GTCTC	5 cut(s)	166, 818, 1493, 1524, 1595
BfaI	CTAG	2 cut(s)	612, 1350
BfmI	CTRYAG	1 cut(s)	1208
BfuAI	ACCTGC	1 cut(s)	1201
BfuI	GTATCC	1 cut(s)	832
BisI	GCNGC	7 cut(s)	440, 978, 1002, 1053, 1262, 1392, 1620
BlnI	CCTAGG	1 cut(s)	1349
BlsI	GCNGC	7 cut(s)	441, 979, 1003, 1054, 1263, 1393, 1621
Bme1390I	CCNGG	4 cut(s)	587, 588, 1039, 1254
Bme18I	GGWCC	2 cut(s)	1307, 1601
BmeT110I	CYCGRG	2 cut(s)	17, 586
BmgT120I	GGNCC	3 cut(s)	71, 1307, 1601
BmiI	GGNNCC	5 cut(s)	1239, 1308, 1386, 1549, 1581
BmrFI	CCNGG	4 cut(s)	587, 588, 1039, 1254
BmsI	GCATC	8 cut(s)	145, 391, 1030, 1166, 1191, 1329, 1363, 1443
BpiI	GAAGAC	2 cut(s)	858, 960
BpmI	CTGGAG	1 cut(s)	1371
BpuEI	CTTGAG	1 cut(s)	302
BpuMI	CCSGG	3 cut(s)	587, 588, 1254
Bsa29I	ATCGAT	1 cut(s)	1024
BsaBI	GATNNNNATC	1 cut(s)	1030
BsaI	GGTCTC	1 cut(s)	1595
BsaJI	CCNNGG	4 cut(s)	437, 585, 586, 1349
BsaWI	WCCGGW	2 cut(s)	638, 1420
BsaXI	ACNNNNNCTCC	4 cut(s)	372, 402, 1033, 1063
Bsc4I	CCNNNNNNNGG	6 cut(s)	283, 592, 973, 1420, 1610, 1640
Bse118I	RCCGGY	2 cut(s)	1312, 1420
Bse1I	ACTGG	2 cut(s)	772, 907
Bse3DI	GCAATG	1 cut(s)	1115
Bse8I	GATNNNNATC	1 cut(s)	1030
BseAI	TCCGGA	1 cut(s)	638
BseBI	CCWGG	1 cut(s)	1039
BseCI	ATCGAT	1 cut(s)	1024
BseDI	CCNNGG	4 cut(s)	437, 585, 586, 1349
BseGI	GGATG	3 cut(s)	1227, 1239, 1354
BseJI	GATNNNNATC	1 cut(s)	1030
BseLI	CCNNNNNNNGG	6 cut(s)	283, 592, 973, 1420, 1610, 1640
BseMI	GCAATG	1 cut(s)	1115
BseMII	CTCAG	4 cut(s)	830, 1086, 1535, 1606
BseNI	ACTGG	2 cut(s)	772, 907
BseRI	GAGGAG	3 cut(s)	483, 912, 1057
BseXI	GCAGC	6 cut(s)	964, 988, 1039, 1273, 1403, 1606
Bsh1236I	CGCG	3 cut(s)	416, 418, 439
BshFI	GGCC	3 cut(s)	73, 442, 1316
BshNI	GGYRCC	2 cut(s)	1237, 1579
BshTI	ACCGGT	1 cut(s)	1420
BshVI	ATCGAT	1 cut(s)	1024
BsiHKAI	GWGCWC	1 cut(s)	96
BsiHKCI	CYCGRG	2 cut(s)	17, 586
BsiSI	CCGG	5 cut(s)	587, 639, 1253, 1313, 1421
BslFI	GGGAC	1 cut(s)	1320
BslI	CCNNNNNNNGG	6 cut(s)	283, 592, 973, 1420, 1610, 1640
BsmAI	GTCTC	5 cut(s)	166, 818, 1493, 1524, 1595
BsmBI	CGTCTC	1 cut(s)	1493
BsmFI	GGGAC	1 cut(s)	1320
BsmI	GAATGC	1 cut(s)	12
BsnI	GGCC	3 cut(s)	73, 442, 1316
Bso31I	GGTCTC	1 cut(s)	1595
BsoBI	CYCGRG	2 cut(s)	17, 586
Bsp1286I	GDGCHC	1 cut(s)	96
Bsp13I	TCCGGA	1 cut(s)	638
BspANI	GGCC	3 cut(s)	73, 442, 1316
BspCNI	CTCAG	4 cut(s)	829, 1085, 1534, 1605
BspDI	ATCGAT	1 cut(s)	1024
BspEI	TCCGGA	1 cut(s)	638
BspFNI	CGCG	3 cut(s)	416, 418, 439
BspHI	TCATGA	1 cut(s)	1491
BspLI	GGNNCC	5 cut(s)	1239, 1308, 1386, 1549, 1581
BspMAI	CTGCAG	1 cut(s)	1212
BspMI	ACCTGC	1 cut(s)	1201
BspPI	GGATC	4 cut(s)	335, 613, 842, 1039
BspQI	GCTCTTC	1 cut(s)	1609
BspT107I	GGYRCC	2 cut(s)	1237, 1579
BspTNI	GGTCTC	1 cut(s)	1595
BsrBI	CCGCTC	1 cut(s)	1360
BsrDI	GCAATG	1 cut(s)	1115
BsrFI	RCCGGY	2 cut(s)	1312, 1420
BsrI	ACTGG	2 cut(s)	772, 907
BssAI	RCCGGY	2 cut(s)	1312, 1420
BssECI	CCNNGG	4 cut(s)	437, 585, 586, 1349
BssT1I	CCWWGG	1 cut(s)	1349
Bst2UI	CCWGG	1 cut(s)	1039
Bst4CI	ACNGT	5 cut(s)	259, 372, 621, 685, 1405
Bst6I	CTCTTC	1 cut(s)	1609
BstC8I	GCNNGC	3 cut(s)	654, 1259, 1314
BstDEI	CTNAG	4 cut(s)	816, 1072, 1521, 1592
BstDSI	CCRYGG	1 cut(s)	437
BstF5I	GGATG	3 cut(s)	1227, 1239, 1354
BstFNI	CGCG	3 cut(s)	416, 418, 439
BstHHI	GCGC	3 cut(s)	418, 652, 1021
BstMAI	GTCTC	5 cut(s)	166, 818, 1493, 1524, 1595
BstMWI	GCNNNNNNNGC	4 cut(s)	1001, 1318, 1327, 1391
BstNI	CCWGG	1 cut(s)	1039
BstNSI	RCATGY	3 cut(s)	1261, 1486, 1510
BstSCI	CCNGG	4 cut(s)	585, 586, 1037, 1252
BstSFI	CTRYAG	1 cut(s)	1208
BstUI	CGCG	3 cut(s)	416, 418, 439
BstV1I	GCAGC	6 cut(s)	964, 988, 1039, 1273, 1403, 1606
BstV2I	GAAGAC	2 cut(s)	858, 960
BstX2I	RGATCY	1 cut(s)	605
BstYI	RGATCY	1 cut(s)	605
Bsu15I	ATCGAT	1 cut(s)	1024
BsuI	GTATCC	1 cut(s)	832
BsuRI	GGCC	3 cut(s)	73, 442, 1316
BsuTUI	ATCGAT	1 cut(s)	1024
BtgI	CCRYGG	1 cut(s)	437
BtsCI	GGATG	3 cut(s)	1227, 1239, 1354
BtsI	GCAGTG	1 cut(s)	1603
BtsIMutI	CAGTG	2 cut(s)	1376, 1603
BveI	ACCTGC	1 cut(s)	1201
Cac8I	GCNNGC	3 cut(s)	654, 1259, 1314
CciI	TCATGA	1 cut(s)	1491
CfoI	GCGC	3 cut(s)	418, 652, 1021
Cfr10I	RCCGGY	2 cut(s)	1312, 1420
Cfr13I	GGNCC	3 cut(s)	71, 1307, 1601
Cfr42I	CCGCGG	1 cut(s)	440
Cfr9I	CCCGGG	1 cut(s)	586
ClaI	ATCGAT	1 cut(s)	1024
CseI	GACGC	2 cut(s)	407, 1475
CspAI	ACCGGT	1 cut(s)	1420
CspCI	CAANNNNNGTGG	2 cut(s)	1407, 1442
CviAII	CATG	7 cut(s)	80, 188, 1258, 1375, 1483, 1492, 1507
DdeI	CTNAG	4 cut(s)	816, 1072, 1521, 1592
Eam1104I	CTCTTC	1 cut(s)	1609
EarI	CTCTTC	1 cut(s)	1609
Ecl136II	GAGCTC	1 cut(s)	94
Eco130I	CCWWGG	1 cut(s)	1349
Eco24I	GRGCYC	1 cut(s)	96
Eco31I	GGTCTC	1 cut(s)	1595
Eco32I	GATATC	1 cut(s)	503
Eco47I	GGWCC	2 cut(s)	1307, 1601
Eco53kI	GAGCTC	1 cut(s)	94
Eco57I	CTGAAG	3 cut(s)	981, 1077, 1181
Eco88I	CYCGRG	2 cut(s)	17, 586
EcoICRI	GAGCTC	1 cut(s)	94
EcoRI	GAATTC	2 cut(s)	4, 226
EcoRII	CCWGG	1 cut(s)	1037
EcoRV	GATATC	1 cut(s)	503
EcoT14I	CCWWGG	1 cut(s)	1349
EcoT22I	ATGCAT	1 cut(s)	1344
EcoT38I	GRGCYC	1 cut(s)	96
ErhI	CCWWGG	1 cut(s)	1349
Esp3I	CGTCTC	1 cut(s)	1493
FaeI	CATG	7 cut(s)	83, 191, 1261, 1378, 1486, 1495, 1510
FaqI	GGGAC	1 cut(s)	1320
FatI	CATG	7 cut(s)	79, 187, 1257, 1374, 1482, 1491, 1506
FauI	CCCGC	2 cut(s)	285, 1223
FbaI	TGATCA	4 cut(s)	163, 190, 523, 1366
Fnu4HI	GCNGC	7 cut(s)	440, 978, 1002, 1053, 1262, 1392, 1620
FokI	GGATG	3 cut(s)	1234, 1246, 1341
FriOI	GRGCYC	1 cut(s)	96
Fsp4HI	GCNGC	7 cut(s)	440, 978, 1002, 1053, 1262, 1392, 1620
FspBI	CTAG	2 cut(s)	612, 1350
GlaI	GCGC	3 cut(s)	417, 651, 1020
GluI	GCNGC	7 cut(s)	440, 978, 1002, 1053, 1262, 1392, 1620
GsuI	CTGGAG	1 cut(s)	1371
HaeIII	GGCC	3 cut(s)	73, 442, 1316
HapII	CCGG	5 cut(s)	587, 639, 1253, 1313, 1421
HgaI	GACGC	2 cut(s)	407, 1475
HhaI	GCGC	3 cut(s)	418, 652, 1021
Hin1II	CATG	7 cut(s)	83, 191, 1261, 1378, 1486, 1495, 1510
Hin6I	GCGC	3 cut(s)	416, 650, 1019
HinP1I	GCGC	3 cut(s)	416, 650, 1019
HinfI	GANTC	9 cut(s)	32, 241, 287, 635, 956, 989, 1045, 1429, 1567
HpaII	CCGG	5 cut(s)	587, 639, 1253, 1313, 1421
HphI	GGTGA	1 cut(s)	426
Hpy166II	GTNNAC	1 cut(s)	1601
Hpy8I	GTNNAC	1 cut(s)	1601
HpyAV	CCTTC	3 cut(s)	342, 379, 1332
HpyCH4III	ACNGT	5 cut(s)	259, 372, 621, 685, 1405
HpyCH4IV	ACGT	2 cut(s)	699, 798
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1001, 1318, 1327, 1391
HpyF3I	CTNAG	4 cut(s)	816, 1072, 1521, 1592
HpySE526I	ACGT	2 cut(s)	699, 798
Hsp92II	CATG	7 cut(s)	83, 191, 1261, 1378, 1486, 1495, 1510
HspAI	GCGC	3 cut(s)	416, 650, 1019
Kpn2I	TCCGGA	1 cut(s)	638
KroI	GCCGGC	1 cut(s)	1312
KroNI	GCCGGC	1 cut(s)	1314
Ksp22I	TGATCA	4 cut(s)	163, 190, 523, 1366
KspI	CCGCGG	1 cut(s)	440
LguI	GCTCTTC	1 cut(s)	1609
LmnI	GCTCC	2 cut(s)	1390, 1553
Lsp1109I	GCAGC	6 cut(s)	964, 988, 1039, 1273, 1403, 1606
LweI	GCATC	8 cut(s)	145, 391, 1030, 1166, 1191, 1329, 1363, 1443
MaeI	CTAG	2 cut(s)	612, 1350
MaeII	ACGT	2 cut(s)	699, 798
MaeIII	GTNAC	2 cut(s)	695, 1191
MbiI	CCGCTC	1 cut(s)	1360
MboII	GAAGA	5 cut(s)	70, 600, 858, 965, 1626
MfeI	CAATTG	3 cut(s)	345, 751, 1176
MflI	RGATCY	1 cut(s)	605
MhlI	GDGCHC	1 cut(s)	96
MlyI	GAGTC	4 cut(s)	41, 644, 950, 1054
MmeI	TCCRAC	2 cut(s)	453, 875
Mph1103I	ATGCAT	1 cut(s)	1344
MroI	TCCGGA	1 cut(s)	638
MroNI	GCCGGC	1 cut(s)	1312
MroXI	GAANNNNTTC	2 cut(s)	595, 1535
MseI	TTAA	6 cut(s)	153, 513, 564, 1098, 1637, 1654
MslI	CAYNNNNRTG	1 cut(s)	1379
MspA1I	CMGCKG	3 cut(s)	439, 461, 1001
MspI	CCGG	5 cut(s)	587, 639, 1253, 1313, 1421
MspR9I	CCNGG	4 cut(s)	587, 588, 1039, 1254
MunI	CAATTG	3 cut(s)	345, 751, 1176
Mva1269I	GAATGC	1 cut(s)	12
MvaI	CCWGG	1 cut(s)	1039
MvnI	CGCG	3 cut(s)	416, 418, 439
MwoI	GCNNNNNNNGC	4 cut(s)	1001, 1318, 1327, 1391
NaeI	GCCGGC	1 cut(s)	1314
NciI	CCSGG	3 cut(s)	587, 588, 1254
NgoMIV	GCCGGC	1 cut(s)	1312
NlaIII	CATG	7 cut(s)	83, 191, 1261, 1378, 1486, 1495, 1510
NlaIV	GGNNCC	5 cut(s)	1239, 1308, 1386, 1549, 1581
NmuCI	GTSAC	1 cut(s)	1191
NsiI	ATGCAT	1 cut(s)	1344
NspI	RCATGY	3 cut(s)	1261, 1486, 1510
PaeI	GCATGC	1 cut(s)	1261
PagI	TCATGA	1 cut(s)	1491
PciSI	GCTCTTC	1 cut(s)	1609
PcsI	WCGNNNNNNNCGW	2 cut(s)	689, 860
PctI	GAATGC	1 cut(s)	12
PdiI	GCCGGC	1 cut(s)	1314
PdmI	GAANNNNTTC	2 cut(s)	595, 1535
PfeI	GAWTC	5 cut(s)	241, 287, 989, 1429, 1567
PflFI	GACNNNGTC	1 cut(s)	1516
PinAI	ACCGGT	1 cut(s)	1420
PkrI	GCNGC	7 cut(s)	441, 979, 1003, 1054, 1263, 1393, 1621
PleI	GAGTC	4 cut(s)	40, 643, 950, 1053
PpsI	GAGTC	4 cut(s)	40, 643, 950, 1053
PshBI	ATTAAT	1 cut(s)	513
Psp124BI	GAGCTC	1 cut(s)	96
Psp1406I	AACGTT	1 cut(s)	798
Psp6I	CCWGG	1 cut(s)	1037
PspGI	CCWGG	1 cut(s)	1037
PspN4I	GGNNCC	5 cut(s)	1239, 1308, 1386, 1549, 1581
PspPI	GGNCC	3 cut(s)	71, 1307, 1601
PstI	CTGCAG	1 cut(s)	1212
PsuI	RGATCY	1 cut(s)	605
PsyI	GACNNNGTC	1 cut(s)	1516
PvuII	CAGCTG	1 cut(s)	1001
RseI	CAYNNNNRTG	1 cut(s)	1379
SacI	GAGCTC	1 cut(s)	96
SacII	CCGCGG	1 cut(s)	440
SapI	GCTCTTC	1 cut(s)	1609
SaqAI	TTAA	6 cut(s)	153, 513, 564, 1098, 1637, 1654
SatI	GCNGC	7 cut(s)	440, 978, 1002, 1053, 1262, 1392, 1620
Sau96I	GGNCC	3 cut(s)	71, 1307, 1601
SchI	GAGTC	4 cut(s)	41, 644, 950, 1054
ScrFI	CCNGG	4 cut(s)	587, 588, 1039, 1254
SduI	GDGCHC	1 cut(s)	96
SfaNI	GCATC	8 cut(s)	145, 391, 1030, 1166, 1191, 1329, 1363, 1443
SfcI	CTRYAG	1 cut(s)	1208
Sfr303I	CCGCGG	1 cut(s)	440
SgrBI	CCGCGG	1 cut(s)	440
SinI	GGWCC	2 cut(s)	1307, 1601
SmaI	CCCGGG	1 cut(s)	588
SmiMI	CAYNNNNRTG	1 cut(s)	1379
SmlI	CTYRAG	1 cut(s)	281
SmoI	CTYRAG	1 cut(s)	281
SphI	GCATGC	1 cut(s)	1261
SspMI	CTAG	2 cut(s)	612, 1350
SstI	GAGCTC	1 cut(s)	96
StyD4I	CCNGG	4 cut(s)	585, 586, 1037, 1252
StyI	CCWWGG	1 cut(s)	1349
TaaI	ACNGT	5 cut(s)	259, 372, 621, 685, 1405
TaiI	ACGT	2 cut(s)	702, 801
TaqI	TCGA	6 cut(s)	139, 170, 294, 692, 1024, 1034
TauI	GCSGC	1 cut(s)	442
TfiI	GAWTC	5 cut(s)	241, 287, 989, 1429, 1567
Tru1I	TTAA	6 cut(s)	153, 513, 564, 1098, 1637, 1654
Tru9I	TTAA	6 cut(s)	153, 513, 564, 1098, 1637, 1654
TscAI	CASTG	2 cut(s)	1376, 1603
TseFI	GTSAC	1 cut(s)	1191
TseI	GCWGC	6 cut(s)	977, 1001, 1052, 1261, 1391, 1619
Tsp45I	GTSAC	1 cut(s)	1191
TspDTI	ATGAA	6 cut(s)	17, 96, 791, 1391, 1508, 1544
TspGWI	ACGGA	2 cut(s)	851, 1099
TspMI	CCCGGG	1 cut(s)	586
TspRI	CASTG	2 cut(s)	1376, 1603
Tth111I	GACNNNGTC	1 cut(s)	1516
VpaK11BI	GGWCC	2 cut(s)	1307, 1601
VspI	ATTAAT	1 cut(s)	513
XapI	RAATTY	3 cut(s)	4, 226, 643
XbaI	TCTAGA	1 cut(s)	611
XceI	RCATGY	3 cut(s)	1261, 1486, 1510
XmaI	CCCGGG	1 cut(s)	586
XmaJI	CCTAGG	1 cut(s)	1349
XmnI	GAANNNNTTC	2 cut(s)	595, 1535
XspI	CTAG	2 cut(s)	612, 1350
Zsp2I	ATGCAT	1 cut(s)	1344

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.