MD09G1146100.v1.1

oxidoreductase At1g06690, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005042
Physical Location & Seq
Reverse (-)
11387967 .. 11393675
5709 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1146100.v1.1.491

Sequence Viewer

Length: 1095 bp
ATGGCTATGCATCTTAGCAGTGCATGTTTTTCTGCTTTGAGTGAGAGGAGAGTTCCTAGGGTTAGAGCTGTTGCTTCTGAGAATGTGACCGCAGAGGATGATAGGGTGAAATTGGGTGGCTCTGATTTGAAGGTGACAAGGCTTGGGATTGGAGCTTGGTCATGGGGTGACACCAGTTATTGGAATAACTTCCAATGGGATGACAGGAAAATGAAAGCAGCTAAGGCTGCTTTCGATGTCAGTGTAGATGGCGGAATAACATTCTTTGATACTGCTGAAGTGTACGGCTCCAGGGCTTCTTTTGGTGCTGTAAATTCTGAAACTCTACTAGGAAGATTTATCAAGGAAAGGAAACAAAAGGATCCAGGAGTAGAAGTTGCTGTTGCGACCAAATTTGCAGCTTTGCCGTGGAGGCTGGGCCGACAAAGTGTCCTGGGCGCCCTTAAGGATTCCCTCAATCGCCTTGAACTTTCTTCGGTAGAACTGTATCAACTCCATTGGCCTGGAATATGGGGAAATAAAGGGTATCTCGATGGTCTTGGAGACGCTGTTGAGCAGGGCCTTGTGAAGGCCGTTGGTGTTTCCAACTACAGCGAAAAGCGATTGCGTGAGGCACACACGAAACTTCAAAAGAGAGGTATCCCACTAGCATCGAATCAAGTGAATTACAGCCTCATATACAGGGCACCAGAGGAGAACGGAGTTAAGTCTACCTGTGATGAACTTGGGATCACTTTGATTGCCTATTCACCTATTGCTCAAGGTGCTCTTACAGGAAAGTATACACCTGAAAATCCCCCAAGTGGCCCCCGAGGACAAATTTATACTCCTGAATTTCTTACAAAGCTCCAACCTCTGCTGAACCGGATCAAGGAAATAGGAGAAAAGTACAGTAAAACAAACACACAGGTAGTCCTAAACTGGCTGATAGCCCAGGACAACGTTGTGCCAATCCCGGGAGCCAAAAACGGAGAACAGGCTGCAGAATTCGCAGGTGCCCTTGGATGGAGGCTCAACAGTGAAGAAGTAGCCGAATTGCGCGCTTTGGCTACAAAGATAAAACCTGTTACTGGTTTCCCTGTTGAAAAATTATAA

Protein Analysis

365

Amino Acids

39.91

Weight (kDa)

9.08

Isoelectric Point (pI)

26.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 47 - 349 1.5e-55 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1093
AarI CACCTGC 1 cut(s) 983
Acc36I ACCTGC 1 cut(s) 983
AccB1I GGYRCC 3 cut(s) 437, 685, 995
AccB7I CCANNNNNTGG 1 cut(s) 180
AccI GTMKAC 2 cut(s) 710, 782
AccII CGCG 1 cut(s) 1041
AciI CCGC 2 cut(s) 90, 252
AclI AACGTT 1 cut(s) 942
AclWI GGATC 4 cut(s) 356, 369, 737, 875
AcsI RAATTY 5 cut(s) 313, 392, 819, 833, 986
AcuI CTGAAG 1 cut(s) 297
AcyI GRCGYC 1 cut(s) 438
AfaI GTAC 2 cut(s) 284, 890
AfiI CCNNNNNNNGG 7 cut(s) 180, 568, 803, 871, 956, 1005, 1070
AflII CTTAAG 1 cut(s) 443
AgsI TTSAA 4 cut(s) 130, 467, 629, 1085
AhdI GACNNNNNGTC 1 cut(s) 428
AjnI CCWGG 5 cut(s) 290, 364, 432, 502, 933
AluBI AGCT 5 cut(s) 68, 155, 221, 401, 847
AluI AGCT 5 cut(s) 68, 155, 221, 401, 847
Alw21I GWGCWC 1 cut(s) 769
Alw26I GTCTC 1 cut(s) 537
AlwI GGATC 4 cut(s) 356, 369, 737, 875
Ama87I CYCGRG 2 cut(s) 810, 955
AoxI GGCC 5 cut(s) 418, 500, 559, 570, 805
ApeKI GCWGC 4 cut(s) 218, 227, 398, 980
ApoI RAATTY 5 cut(s) 313, 392, 819, 833, 986
Asp700I GAANNNNTTC 1 cut(s) 188
AspA2I CCTAGG 1 cut(s) 56
AspLEI GCGC 3 cut(s) 440, 1041, 1043
AspS9I GGNCC 3 cut(s) 418, 559, 806
AsuC2I CCSGG 2 cut(s) 956, 957
AsuHPI GGTGA 4 cut(s) 118, 145, 179, 741
AvaI CYCGRG 2 cut(s) 810, 955
AvrII CCTAGG 1 cut(s) 56
BaeGI GKGCMC 2 cut(s) 688, 1000
BamHI GGATCC 1 cut(s) 361
BanI GGYRCC 3 cut(s) 437, 685, 995
Bbv12I GWGCWC 1 cut(s) 769
BbvI GCAGC 4 cut(s) 214, 230, 410, 967
BccI CCATC 3 cut(s) 242, 527, 999
BceAI ACGGC 3 cut(s) 301, 391, 557
BciT130I CCWGG 5 cut(s) 292, 366, 434, 504, 935
BciVI GTATCC 1 cut(s) 650
BcnI CCSGG 2 cut(s) 956, 957
BcoDI GTCTC 1 cut(s) 537
BfaI CTAG 3 cut(s) 57, 329, 647
BfmI CTRYAG 2 cut(s) 589, 981
BfoI RGCGCY 1 cut(s) 441
BfrI CTTAAG 1 cut(s) 443
BfuAI ACCTGC 1 cut(s) 983
BfuI GTATCC 1 cut(s) 650
BglI GCCNNNNNGGC 1 cut(s) 412
BisI GCNGC 4 cut(s) 219, 228, 399, 981
BlnI CCTAGG 1 cut(s) 56
BlsI GCNGC 4 cut(s) 220, 229, 400, 982
Bme1390I CCNGG 7 cut(s) 292, 366, 434, 504, 935, 956, 957
BmeRI GACNNNNNGTC 1 cut(s) 428
BmeT110I CYCGRG 2 cut(s) 810, 955
BmgT120I GGNCC 3 cut(s) 418, 559, 806
BmiI GGNNCC 7 cut(s) 289, 363, 439, 687, 808, 961, 997
BmrFI CCNGG 7 cut(s) 292, 366, 434, 504, 935, 956, 957
BmsI GCATC 2 cut(s) 19, 659
BpmI CTGGAG 1 cut(s) 274
Bpu10I CCTNAGC 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 744
BpuMI CCSGG 2 cut(s) 956, 957
BsaHI GRCGYC 1 cut(s) 438
BsaJI CCNNGG 8 cut(s) 56, 291, 407, 433, 811, 933, 955, 1000
BsaWI WCCGGW 1 cut(s) 864
BsaXI ACNNNNNCTCC 4 cut(s) 360, 390, 534, 564
Bsc4I CCNNNNNNNGG 7 cut(s) 180, 568, 803, 871, 956, 1005, 1070
Bse1I ACTGG 3 cut(s) 174, 926, 1075
BseBI CCWGG 5 cut(s) 292, 366, 434, 504, 935
BseDI CCNNGG 8 cut(s) 56, 291, 407, 433, 811, 933, 955, 1000
BseGI GGATG 3 cut(s) 103, 205, 1010
BseLI CCNNNNNNNGG 7 cut(s) 180, 568, 803, 871, 956, 1005, 1070
BseMII CTCAG 1 cut(s) 69
BseNI ACTGG 3 cut(s) 174, 926, 1075
BsePI GCGCGC 1 cut(s) 1039
BseRI GAGGAG 2 cut(s) 61, 707
BseSI GKGCMC 2 cut(s) 688, 1000
BseXI GCAGC 4 cut(s) 214, 230, 410, 967
BseYI CCCAGC 1 cut(s) 415
Bsh1236I CGCG 1 cut(s) 1041
BshFI GGCC 5 cut(s) 420, 502, 561, 572, 807
BshNI GGYRCC 3 cut(s) 437, 685, 995
BsiHKAI GWGCWC 1 cut(s) 769
BsiHKCI CYCGRG 2 cut(s) 810, 955
BsiSI CCGG 2 cut(s) 865, 956
BslI CCNNNNNNNGG 7 cut(s) 180, 568, 803, 871, 956, 1005, 1070
BsmAI GTCTC 1 cut(s) 537
BsmBI CGTCTC 1 cut(s) 537
BsnI GGCC 5 cut(s) 420, 502, 561, 572, 807
BsoBI CYCGRG 2 cut(s) 810, 955
Bsp1286I GDGCHC 3 cut(s) 688, 769, 1000
Bsp143I GATC 3 cut(s) 361, 729, 867
BspACI CCGC 2 cut(s) 90, 252
BspANI GGCC 5 cut(s) 420, 502, 561, 572, 807
BspCNI CTCAG 1 cut(s) 70
BspFNI CGCG 1 cut(s) 1041
BspLI GGNNCC 7 cut(s) 289, 363, 439, 687, 808, 961, 997
BspMAI CTGCAG 1 cut(s) 985
BspMI ACCTGC 1 cut(s) 983
BspPI GGATC 4 cut(s) 356, 369, 737, 875
BspT107I GGYRCC 3 cut(s) 437, 685, 995
BspTI CTTAAG 1 cut(s) 443
BsrI ACTGG 3 cut(s) 174, 926, 1075
BssECI CCNNGG 8 cut(s) 56, 291, 407, 433, 811, 933, 955, 1000
BssHII GCGCGC 1 cut(s) 1039
BssMI GATC 3 cut(s) 361, 729, 867
BssNAI GTATAC 1 cut(s) 783
BssNI GRCGYC 1 cut(s) 438
BssT1I CCWWGG 2 cut(s) 56, 1000
Bst1107I GTATAC 1 cut(s) 783
Bst2UI CCWGG 5 cut(s) 292, 366, 434, 504, 935
Bst4CI ACNGT 3 cut(s) 486, 893, 1019
BstACI GRCGYC 1 cut(s) 438
BstAFI CTTAAG 1 cut(s) 443
BstC8I GCNNGC 1 cut(s) 1041
BstDEI CTNAG 3 cut(s) 14, 78, 222
BstDSI CCRYGG 1 cut(s) 407
BstENI CCTNNNNNAGG 1 cut(s) 566
BstF5I GGATG 3 cut(s) 103, 205, 1010
BstFNI CGCG 1 cut(s) 1041
BstH2I RGCGCY 1 cut(s) 441
BstHHI GCGC 3 cut(s) 440, 1041, 1043
BstKTI GATC 3 cut(s) 364, 732, 870
BstMAI GTCTC 1 cut(s) 537
BstMBI GATC 3 cut(s) 361, 729, 867
BstMWI GCNNNNNNNGC 5 cut(s) 224, 227, 412, 764, 989
BstNI CCWGG 5 cut(s) 292, 366, 434, 504, 935
BstNSI RCATGY 1 cut(s) 27
BstSCI CCNGG 7 cut(s) 290, 364, 432, 502, 933, 954, 955
BstSFI CTRYAG 2 cut(s) 589, 981
BstSLI GKGCMC 2 cut(s) 688, 1000
BstUI CGCG 1 cut(s) 1041
BstV1I GCAGC 4 cut(s) 214, 230, 410, 967
BstX2I RGATCY 1 cut(s) 361
BstXI CCANNNNNNTGG 1 cut(s) 503
BstYI RGATCY 1 cut(s) 361
BstZ17I GTATAC 1 cut(s) 783
BsuI GTATCC 1 cut(s) 650
BsuRI GGCC 5 cut(s) 420, 502, 561, 572, 807
BtgI CCRYGG 1 cut(s) 407
BtsCI GGATG 3 cut(s) 103, 205, 1010
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 3 cut(s) 25, 247, 1024
BveI ACCTGC 1 cut(s) 983
Cac8I GCNNGC 1 cut(s) 1041
CfoI GCGC 3 cut(s) 440, 1041, 1043
Cfr13I GGNCC 3 cut(s) 418, 559, 806
Cfr9I CCCGGG 1 cut(s) 955
CseI GACGC 1 cut(s) 554
Csp6I GTAC 2 cut(s) 283, 889
CviAII CATG 2 cut(s) 24, 162
CviQI GTAC 2 cut(s) 283, 889
DdeI CTNAG 3 cut(s) 14, 78, 222
DinI GGCGCC 1 cut(s) 439
DpnI GATC 3 cut(s) 363, 731, 869
DpnII GATC 3 cut(s) 361, 729, 867
DriI GACNNNNNGTC 1 cut(s) 428
Eam1105I GACNNNNNGTC 1 cut(s) 428
EciI GGCGGA 1 cut(s) 267
Eco130I CCWWGG 2 cut(s) 56, 1000
Eco57I CTGAAG 1 cut(s) 297
Eco88I CYCGRG 2 cut(s) 810, 955
EcoNI CCTNNNNNAGG 1 cut(s) 566
EcoO109I RGGNCCY 1 cut(s) 559
EcoRI GAATTC 1 cut(s) 986
EcoRII CCWGG 5 cut(s) 290, 364, 432, 502, 933
EcoT14I CCWWGG 2 cut(s) 56, 1000
EcoT22I ATGCAT 1 cut(s) 12
EgeI GGCGCC 1 cut(s) 439
EheI GGCGCC 1 cut(s) 439
ErhI CCWWGG 2 cut(s) 56, 1000
Esp3I CGTCTC 1 cut(s) 537
FaeI CATG 2 cut(s) 27, 165
FaiI YATR 9 cut(s) 8, 25, 163, 511, 677, 679, 783, 825, 1093
FalI AAGNNNNNCTT 2 cut(s) 753, 785
FatI CATG 2 cut(s) 23, 161
FblI GTMKAC 2 cut(s) 710, 782
Fnu4HI GCNGC 4 cut(s) 219, 228, 399, 981
FokI GGATG 3 cut(s) 110, 212, 1017
Fsp4HI GCNGC 4 cut(s) 219, 228, 399, 981
FspBI CTAG 3 cut(s) 57, 329, 647
GlaI GCGC 3 cut(s) 439, 1040, 1042
GluI GCNGC 4 cut(s) 219, 228, 399, 981
GsaI CCCAGC 1 cut(s) 419
GsuI CTGGAG 1 cut(s) 274
HaeII RGCGCY 1 cut(s) 441
HaeIII GGCC 5 cut(s) 420, 502, 561, 572, 807
HapII CCGG 2 cut(s) 865, 956
HgaI GACGC 1 cut(s) 554
HhaI GCGC 3 cut(s) 440, 1041, 1043
Hin1I GRCGYC 1 cut(s) 438
Hin1II CATG 2 cut(s) 27, 165
Hin6I GCGC 3 cut(s) 438, 1039, 1041
HinP1I GCGC 3 cut(s) 438, 1039, 1041
HinfI GANTC 2 cut(s) 449, 655
HpaII CCGG 2 cut(s) 865, 956
HphI GGTGA 4 cut(s) 118, 145, 179, 741
Hpy166II GTNNAC 3 cut(s) 283, 711, 783
Hpy188I TCNGA 3 cut(s) 79, 124, 319
Hpy188III TCNNGA 2 cut(s) 530, 830
Hpy8I GTNNAC 3 cut(s) 283, 711, 783
HpyAV CCTTC 2 cut(s) 124, 562
HpyCH4III ACNGT 3 cut(s) 486, 893, 1019
HpyCH4IV ACGT 1 cut(s) 942
HpyCH4V TGCA 4 cut(s) 10, 23, 398, 983
HpyF10VI GCNNNNNNNGC 5 cut(s) 224, 227, 412, 764, 989
HpyF3I CTNAG 3 cut(s) 14, 78, 222
HpySE526I ACGT 1 cut(s) 942
Hsp92I GRCGYC 1 cut(s) 438
Hsp92II CATG 2 cut(s) 27, 165
HspAI GCGC 3 cut(s) 438, 1039, 1041
KasI GGCGCC 1 cut(s) 437
Kzo9I GATC 3 cut(s) 361, 729, 867
LmnI GCTCC 4 cut(s) 152, 293, 852, 959
Lsp1109I GCAGC 4 cut(s) 214, 230, 410, 967
LweI GCATC 2 cut(s) 19, 659
MaeI CTAG 3 cut(s) 57, 329, 647
MaeII ACGT 1 cut(s) 942
MaeIII GTNAC 4 cut(s) 85, 133, 167, 1066
MalI GATC 3 cut(s) 363, 731, 869
MboI GATC 3 cut(s) 361, 729, 867
MboII GAAGA 3 cut(s) 345, 465, 1034
MflI RGATCY 1 cut(s) 361
MhlI GDGCHC 3 cut(s) 688, 769, 1000
MluCI AATT 9 cut(s) 110, 313, 392, 664, 819, 833, 986, 1034, 1088
Mly113I GGCGCC 1 cut(s) 438
MmeI TCCRAC 2 cut(s) 609, 874
Mph1103I ATGCAT 1 cut(s) 12
MroXI GAANNNNTTC 1 cut(s) 188
MseI TTAA 2 cut(s) 444, 705
MspCI CTTAAG 1 cut(s) 443
MspI CCGG 2 cut(s) 865, 956
MspR9I CCNGG 7 cut(s) 292, 366, 434, 504, 935, 956, 957
MvaI CCWGG 5 cut(s) 292, 366, 434, 504, 935
MvnI CGCG 1 cut(s) 1041
MwoI GCNNNNNNNGC 5 cut(s) 224, 227, 412, 764, 989
NarI GGCGCC 1 cut(s) 438
NciI CCSGG 2 cut(s) 956, 957
NdeII GATC 3 cut(s) 361, 729, 867
NlaIII CATG 2 cut(s) 27, 165
NlaIV GGNNCC 7 cut(s) 289, 363, 439, 687, 808, 961, 997
NmuCI GTSAC 3 cut(s) 85, 133, 167
NsiI ATGCAT 1 cut(s) 12
NspI RCATGY 1 cut(s) 27
PaqCI CACCTGC 1 cut(s) 983
PauI GCGCGC 1 cut(s) 1039
PdmI GAANNNNTTC 1 cut(s) 188
PfeI GAWTC 2 cut(s) 449, 655
PflMI CCANNNNNTGG 1 cut(s) 180
PfoI TCCNGGA 1 cut(s) 364
PkrI GCNGC 4 cut(s) 220, 229, 400, 982
PluTI GGCGCC 1 cut(s) 441
PsiI TTATAA 1 cut(s) 1093
Psp1406I AACGTT 1 cut(s) 942
Psp6I CCWGG 5 cut(s) 290, 364, 432, 502, 933
PspFI CCCAGC 1 cut(s) 415
PspGI CCWGG 5 cut(s) 290, 364, 432, 502, 933
PspN4I GGNNCC 7 cut(s) 289, 363, 439, 687, 808, 961, 997
PspPI GGNCC 3 cut(s) 418, 559, 806
PstI CTGCAG 1 cut(s) 985
PsuI RGATCY 1 cut(s) 361
PteI GCGCGC 1 cut(s) 1039
RsaI GTAC 2 cut(s) 284, 890
RsaNI GTAC 2 cut(s) 283, 889
SaqAI TTAA 2 cut(s) 444, 705
SatI GCNGC 4 cut(s) 219, 228, 399, 981
Sau3AI GATC 3 cut(s) 361, 729, 867
Sau96I GGNCC 3 cut(s) 418, 559, 806
ScrFI CCNGG 7 cut(s) 292, 366, 434, 504, 935, 956, 957
SduI GDGCHC 3 cut(s) 688, 769, 1000
SfaNI GCATC 2 cut(s) 19, 659
SfcI CTRYAG 2 cut(s) 589, 981
SfoI GGCGCC 1 cut(s) 439
SmaI CCCGGG 1 cut(s) 957
SmlI CTYRAG 2 cut(s) 443, 759
SmoI CTYRAG 2 cut(s) 443, 759
Sse9I AATT 9 cut(s) 110, 313, 392, 664, 819, 833, 986, 1034, 1088
SsiI CCGC 2 cut(s) 90, 252
SspDI GGCGCC 1 cut(s) 437
SspMI CTAG 3 cut(s) 57, 329, 647
StyD4I CCNGG 7 cut(s) 290, 364, 432, 502, 933, 954, 955
StyI CCWWGG 2 cut(s) 56, 1000
TaaI ACNGT 3 cut(s) 486, 893, 1019
TaiI ACGT 1 cut(s) 945
TaqI TCGA 3 cut(s) 234, 531, 653
TasI AATT 9 cut(s) 110, 313, 392, 664, 819, 833, 986, 1034, 1088
TatI WGTACW 1 cut(s) 888
TfiI GAWTC 2 cut(s) 449, 655
Tru1I TTAA 2 cut(s) 444, 705
Tru9I TTAA 2 cut(s) 444, 705
TscAI CASTG 3 cut(s) 25, 247, 1024
TseFI GTSAC 3 cut(s) 85, 133, 167
TseI GCWGC 4 cut(s) 218, 227, 398, 980
Tsp45I GTSAC 3 cut(s) 85, 133, 167
TspDTI ATGAA 2 cut(s) 227, 735
TspGWI ACGGA 2 cut(s) 714, 984
TspMI CCCGGG 1 cut(s) 955
TspRI CASTG 3 cut(s) 25, 247, 1024
Van91I CCANNNNNTGG 1 cut(s) 180
Vha464I CTTAAG 1 cut(s) 443
XagI CCTNNNNNAGG 1 cut(s) 566
XapI RAATTY 5 cut(s) 313, 392, 819, 833, 986
XceI RCATGY 1 cut(s) 27
XmaI CCCGGG 1 cut(s) 955
XmaJI CCTAGG 1 cut(s) 56
XmiI GTMKAC 2 cut(s) 710, 782
XmnI GAANNNNTTC 1 cut(s) 188
XspI CTAG 3 cut(s) 57, 329, 647
Zsp2I ATGCAT 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.