RLG00000020641

oxidoreductase At1g06690, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005042
Physical Location & Seq
Forward (+)
67037347 .. 67041041
3695 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020641

Sequence Viewer

Length: 1179 bp
ATGGCTTTGCATGTTAGCAGTGCAGGGTTTTCTATATTTAGTCACAGGAGAGTTCACAGAGTTAGAGCTGTTGCTTCTGAAAATGTGAGCGTAGAGGAACAGAAGGTGAAGTTGGGGGCGTCTGAGTTGAAGGTGACAAAGCTTGGGATTGGAGCTTGGTCTTGGGGTGACACCAGTTATTGGAATAACTTTGAATGGGATGATAGGAAGATGAAGGCTGCTAAGAATGCGTTTGATGCCAGCATTGATGGTGGCATAACCTTCTTTGATACTGCTGAAGTTTATGGCTCCAGGGCTTCTTTCGGTGCTATAAATTCTGAAACTTTACTAGGAAGATTTATCAAGGAAAGGAAAGAAAAGGATCCAGAAGTGGAGGTTGCTGTTGCAACCAAATTCGCAGCTTTGCCTTGGAGGCTGGGTCGGCAGAGCGTCCTCTCTGCCCTCAAGGATTCCCTCAGTCGCCTCGGTCTTTCTTCAGTAGAACTGTATCAACTCCACTGGCCAGGAGTATGGGGAAATGAAGGATACCTTGATGGTCTTGGAGATGCTGTTGAGCAAGGCCTTGTGAAGGCAGTTGGTGTCTCAAACTATAGAGAAAAGAGATTGCGTGATGCATACGAGAAACTCAAAAAGAGAGGTATCCCACTAGCATCAAACCAAGTGAATTACAGCCTCATATACAGGGCACCAGAGGAGAATGGAGTTAAGGCTACCTGTGACGAACTTGGGATCACTTTGATTGCCTATTCACCTATTGCTCAAGGTGTTCTTACAGGAAAGTATACACCTGAAAATATTCCAACTGGCCCTCGAGGCCAGATTTACACTCGTGATTTTCTTACAAAGGTAAACCATATTTACTTATTCAGCATTTTGAATGCTGAATACACAGTACCAATTTTATTCCTGAAATATCATGGTGATGTGCAGCTCCAACCTCTGCTTAACAGAATCAAGGAAATAGGAGAGAAGTACAGTAAAACACCTACACAGGTAGCCTTAAACTGGCTAATAACCCAGGACAATGTCGTGCCAATTCCAGGAGCTAAAAATGCAGAACAGGCTATGGAATTTGCGGGTGCACTTGGATGGAAACTCAACAATGATGAAGTAACTGAGTTGCGCTCTTTGGCTTCCGAGATTAAACCTGTAACTGGTTTCCCTGTTGAAAACTTATAG

Protein Analysis

393

Amino Acids

43.56

Weight (kDa)

8.34

Isoelectric Point (pI)

25.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 47 - 377 5.4e-55 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 810
AccB1I GGYRCC 1 cut(s) 685
AccB7I CCANNNNNTGG 1 cut(s) 180
AccI GTMKAC 1 cut(s) 782
AciI CCGC 1 cut(s) 1076
AclWI GGATC 3 cut(s) 356, 369, 737
AcoI YGGCCR 1 cut(s) 500
AcsI RAATTY 3 cut(s) 313, 392, 1070
AcuI CTGAAG 2 cut(s) 297, 459
AcyI GRCGYC 1 cut(s) 119
AfaI GTAC 2 cut(s) 894, 974
AfiI CCNNNNNNNGG 5 cut(s) 180, 568, 1005, 1040, 1154
AgsI TTSAA 4 cut(s) 130, 194, 877, 1169
AjnI CCWGG 4 cut(s) 290, 502, 1017, 1039
AjuI GAANNNNNNNTTGG 2 cut(s) 95, 127
AluBI AGCT 6 cut(s) 68, 142, 155, 401, 931, 1046
AluI AGCT 6 cut(s) 68, 142, 155, 401, 931, 1046
Alw21I GWGCWC 1 cut(s) 1084
Alw26I GTCTC 1 cut(s) 586
Alw44I GTGCAC 1 cut(s) 1080
AlwI GGATC 3 cut(s) 356, 369, 737
Ama87I CYCGRG 1 cut(s) 810
AoxI GGCC 4 cut(s) 500, 559, 805, 814
ApaLI GTGCAC 1 cut(s) 1080
ApeKI GCWGC 3 cut(s) 218, 398, 928
ApoI RAATTY 3 cut(s) 313, 392, 1070
Asp700I GAANNNNTTC 1 cut(s) 795
AspLEI GCGC 1 cut(s) 1125
AspS9I GGNCC 1 cut(s) 806
AsuHPI GGTGA 5 cut(s) 118, 145, 179, 741, 932
AvaI CYCGRG 1 cut(s) 810
BaeGI GKGCMC 2 cut(s) 688, 1084
BalI TGGCCA 1 cut(s) 502
BamHI GGATCC 1 cut(s) 361
BanI GGYRCC 1 cut(s) 685
BauI CACGAG 1 cut(s) 828
Bbv12I GWGCWC 1 cut(s) 1084
BbvI GCAGC 3 cut(s) 205, 410, 940
BccI CCATC 3 cut(s) 242, 527, 1083
BciT130I CCWGG 4 cut(s) 292, 504, 1019, 1041
BciVI GTATCC 2 cut(s) 518, 650
BcoDI GTCTC 1 cut(s) 586
BfaI CTAG 2 cut(s) 329, 647
BfmI CTRYAG 1 cut(s) 589
BfuI GTATCC 2 cut(s) 518, 650
BglI GCCNNNNNGGC 2 cut(s) 412, 813
BisI GCNGC 3 cut(s) 219, 399, 929
BlsI GCNGC 3 cut(s) 220, 400, 930
Bme1390I CCNGG 4 cut(s) 292, 504, 1019, 1041
BmeT110I CYCGRG 1 cut(s) 810
BmgT120I GGNCC 1 cut(s) 806
BmiI GGNNCC 3 cut(s) 289, 363, 687
BmrFI CCNGG 4 cut(s) 292, 504, 1019, 1041
BmsI GCATC 4 cut(s) 226, 535, 601, 659
BplI GAGNNNNNCTC 2 cut(s) 1109, 1141
BpmI CTGGAG 1 cut(s) 274
BpuEI CTTGAG 2 cut(s) 428, 744
BsaHI GRCGYC 1 cut(s) 119
BsaJI CCNNGG 4 cut(s) 291, 407, 463, 1017
BsaXI ACNNNNNCTCC 4 cut(s) 403, 433, 534, 564
Bsc4I CCNNNNNNNGG 5 cut(s) 180, 568, 1005, 1040, 1154
Bse1I ACTGG 5 cut(s) 174, 503, 808, 1010, 1159
BseBI CCWGG 4 cut(s) 292, 504, 1019, 1041
BseDI CCNNGG 4 cut(s) 291, 407, 463, 1017
BseGI GGATG 2 cut(s) 205, 1094
BseLI CCNNNNNNNGG 5 cut(s) 180, 568, 1005, 1040, 1154
BseMII CTCAG 3 cut(s) 114, 469, 1107
BseNI ACTGG 5 cut(s) 174, 503, 808, 1010, 1159
BseRI GAGGAG 1 cut(s) 707
BseSI GKGCMC 2 cut(s) 688, 1084
BseXI GCAGC 3 cut(s) 205, 410, 940
BseYI CCCAGC 1 cut(s) 415
BsgI GTGCAG 2 cut(s) 42, 947
BshFI GGCC 4 cut(s) 502, 561, 807, 816
BshNI GGYRCC 1 cut(s) 685
BsiHKAI GWGCWC 1 cut(s) 1084
BsiHKCI CYCGRG 1 cut(s) 810
BslI CCNNNNNNNGG 5 cut(s) 180, 568, 1005, 1040, 1154
BsmAI GTCTC 1 cut(s) 586
BsmI GAATGC 2 cut(s) 232, 883
BsnI GGCC 4 cut(s) 502, 561, 807, 816
BsoBI CYCGRG 1 cut(s) 810
Bsp1286I GDGCHC 2 cut(s) 688, 1084
Bsp143I GATC 2 cut(s) 361, 729
BspACI CCGC 1 cut(s) 1076
BspANI GGCC 4 cut(s) 502, 561, 807, 816
BspCNI CTCAG 3 cut(s) 115, 468, 1108
BspLI GGNNCC 3 cut(s) 289, 363, 687
BspPI GGATC 3 cut(s) 356, 369, 737
BspT107I GGYRCC 1 cut(s) 685
BsrI ACTGG 5 cut(s) 174, 503, 808, 1010, 1159
BssECI CCNNGG 4 cut(s) 291, 407, 463, 1017
BssMI GATC 2 cut(s) 361, 729
BssNAI GTATAC 1 cut(s) 783
BssNI GRCGYC 1 cut(s) 119
BssSI CACGAG 1 cut(s) 828
BssT1I CCWWGG 1 cut(s) 407
Bst1107I GTATAC 1 cut(s) 783
Bst2BI CACGAG 1 cut(s) 828
Bst2UI CCWGG 4 cut(s) 292, 504, 1019, 1041
Bst4CI ACNGT 3 cut(s) 486, 892, 977
BstACI GRCGYC 1 cut(s) 119
BstC8I GCNNGC 1 cut(s) 241
BstDEI CTNAG 4 cut(s) 123, 222, 455, 1116
BstENI CCTNNNNNAGG 1 cut(s) 566
BstF5I GGATG 2 cut(s) 205, 1094
BstHHI GCGC 1 cut(s) 1125
BstKTI GATC 2 cut(s) 364, 732
BstMAI GTCTC 1 cut(s) 586
BstMBI GATC 2 cut(s) 361, 729
BstMWI GCNNNNNNNGC 7 cut(s) 227, 236, 412, 421, 813, 1052, 1061
BstNI CCWGG 4 cut(s) 292, 504, 1019, 1041
BstNSI RCATGY 1 cut(s) 14
BstSCI CCNGG 4 cut(s) 290, 502, 1017, 1039
BstSFI CTRYAG 1 cut(s) 589
BstSLI GKGCMC 2 cut(s) 688, 1084
BstV1I GCAGC 3 cut(s) 205, 410, 940
BstX2I RGATCY 1 cut(s) 361
BstXI CCANNNNNNTGG 1 cut(s) 510
BstYI RGATCY 1 cut(s) 361
BstZ17I GTATAC 1 cut(s) 783
BsuI GTATCC 2 cut(s) 518, 650
BsuRI GGCC 4 cut(s) 502, 561, 807, 816
BtsCI GGATG 2 cut(s) 205, 1094
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 2 cut(s) 25, 496
Cac8I GCNNGC 1 cut(s) 241
CfoI GCGC 1 cut(s) 1125
Cfr13I GGNCC 1 cut(s) 806
CseI GACGC 2 cut(s) 108, 418
Csp6I GTAC 2 cut(s) 893, 973
CviAII CATG 2 cut(s) 11, 917
CviQI GTAC 2 cut(s) 893, 973
DdeI CTNAG 4 cut(s) 123, 222, 455, 1116
DpnI GATC 2 cut(s) 363, 731
DpnII GATC 2 cut(s) 361, 729
EaeI YGGCCR 1 cut(s) 500
Eco130I CCWWGG 1 cut(s) 407
Eco147I AGGCCT 1 cut(s) 561
Eco57I CTGAAG 2 cut(s) 297, 459
Eco88I CYCGRG 1 cut(s) 810
EcoNI CCTNNNNNAGG 1 cut(s) 566
EcoRII CCWGG 4 cut(s) 290, 502, 1017, 1039
EcoT14I CCWWGG 1 cut(s) 407
EcoT22I ATGCAT 1 cut(s) 616
ErhI CCWWGG 1 cut(s) 407
FaeI CATG 2 cut(s) 14, 920
FalI AAGNNNNNCTT 4 cut(s) 513, 545, 753, 785
FatI CATG 2 cut(s) 10, 916
FauI CCCGC 1 cut(s) 1069
FblI GTMKAC 1 cut(s) 782
Fnu4HI GCNGC 3 cut(s) 219, 399, 929
FokI GGATG 2 cut(s) 212, 1101
Fsp4HI GCNGC 3 cut(s) 219, 399, 929
FspBI CTAG 2 cut(s) 329, 647
GlaI GCGC 1 cut(s) 1124
GluI GCNGC 3 cut(s) 219, 399, 929
GsaI CCCAGC 1 cut(s) 419
GsuI CTGGAG 1 cut(s) 274
HaeIII GGCC 4 cut(s) 502, 561, 807, 816
HgaI GACGC 2 cut(s) 108, 418
HhaI GCGC 1 cut(s) 1125
Hin1I GRCGYC 1 cut(s) 119
Hin1II CATG 2 cut(s) 14, 920
Hin6I GCGC 1 cut(s) 1123
HinP1I GCGC 1 cut(s) 1123
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 2 cut(s) 449, 951
HphI GGTGA 5 cut(s) 118, 145, 179, 741, 932
Hpy166II GTNNAC 4 cut(s) 55, 783, 850, 1082
Hpy188I TCNGA 4 cut(s) 79, 124, 319, 1138
Hpy188III TCNNGA 3 cut(s) 365, 830, 907
Hpy8I GTNNAC 4 cut(s) 55, 783, 850, 1082
HpyAV CCTTC 6 cut(s) 97, 124, 208, 271, 515, 562
HpyCH4III ACNGT 3 cut(s) 486, 892, 977
HpyCH4V TGCA 7 cut(s) 10, 23, 386, 614, 928, 1055, 1082
HpyF10VI GCNNNNNNNGC 7 cut(s) 227, 236, 412, 421, 813, 1052, 1061
HpyF3I CTNAG 4 cut(s) 123, 222, 455, 1116
Hsp92I GRCGYC 1 cut(s) 119
Hsp92II CATG 2 cut(s) 14, 920
HspAI GCGC 1 cut(s) 1123
Kzo9I GATC 2 cut(s) 361, 729
LmnI GCTCC 4 cut(s) 152, 293, 936, 1043
Lsp1109I GCAGC 3 cut(s) 205, 410, 940
LweI GCATC 4 cut(s) 226, 535, 601, 659
MaeI CTAG 2 cut(s) 329, 647
MaeIII GTNAC 6 cut(s) 41, 133, 167, 716, 1111, 1150
MalI GATC 2 cut(s) 363, 731
MboI GATC 2 cut(s) 361, 729
MboII GAAGA 3 cut(s) 220, 345, 465
MflI RGATCY 1 cut(s) 361
MhlI GDGCHC 2 cut(s) 688, 1084
MlsI TGGCCA 1 cut(s) 502
MluCI AATT 6 cut(s) 313, 392, 664, 897, 1035, 1070
MluNI TGGCCA 1 cut(s) 502
MmeI TCCRAC 2 cut(s) 824, 958
Mox20I TGGCCA 1 cut(s) 502
Mph1103I ATGCAT 1 cut(s) 616
MroXI GAANNNNTTC 1 cut(s) 795
MscI TGGCCA 1 cut(s) 502
MseI TTAA 4 cut(s) 705, 945, 1001, 1143
MslI CAYNNNNRTG 2 cut(s) 921, 1087
Msp20I TGGCCA 1 cut(s) 502
MspR9I CCNGG 4 cut(s) 292, 504, 1019, 1041
Mva1269I GAATGC 2 cut(s) 232, 883
MvaI CCWGG 4 cut(s) 292, 504, 1019, 1041
MwoI GCNNNNNNNGC 7 cut(s) 227, 236, 412, 421, 813, 1052, 1061
NdeII GATC 2 cut(s) 361, 729
NlaIII CATG 2 cut(s) 14, 920
NlaIV GGNNCC 3 cut(s) 289, 363, 687
NmuCI GTSAC 4 cut(s) 41, 133, 167, 716
NsiI ATGCAT 1 cut(s) 616
NspI RCATGY 1 cut(s) 14
PaeR7I CTCGAG 1 cut(s) 810
PceI AGGCCT 1 cut(s) 561
PctI GAATGC 2 cut(s) 232, 883
PdmI GAANNNNTTC 1 cut(s) 795
PfeI GAWTC 2 cut(s) 449, 951
PflFI GACNNNGTC 1 cut(s) 1025
PflMI CCANNNNNTGG 1 cut(s) 180
PfoI TCCNGGA 1 cut(s) 1039
PkrI GCNGC 3 cut(s) 220, 400, 930
Psp6I CCWGG 4 cut(s) 290, 502, 1017, 1039
PspFI CCCAGC 1 cut(s) 415
PspGI CCWGG 4 cut(s) 290, 502, 1017, 1039
PspN4I GGNNCC 3 cut(s) 289, 363, 687
PspPI GGNCC 1 cut(s) 806
PspXI VCTCGAGB 1 cut(s) 810
PsuI RGATCY 1 cut(s) 361
PsyI GACNNNGTC 1 cut(s) 1025
RsaI GTAC 2 cut(s) 894, 974
RsaNI GTAC 2 cut(s) 893, 973
RseI CAYNNNNRTG 2 cut(s) 921, 1087
SaqAI TTAA 4 cut(s) 705, 945, 1001, 1143
SatI GCNGC 3 cut(s) 219, 399, 929
Sau3AI GATC 2 cut(s) 361, 729
Sau96I GGNCC 1 cut(s) 806
ScrFI CCNGG 4 cut(s) 292, 504, 1019, 1041
SduI GDGCHC 2 cut(s) 688, 1084
SfaNI GCATC 4 cut(s) 226, 535, 601, 659
SfcI CTRYAG 1 cut(s) 589
SfiI GGCCNNNNNGGCC 1 cut(s) 813
Sfr274I CTCGAG 1 cut(s) 810
SlaI CTCGAG 1 cut(s) 810
SmiMI CAYNNNNRTG 2 cut(s) 921, 1087
SmlI CTYRAG 3 cut(s) 443, 759, 810
SmoI CTYRAG 3 cut(s) 443, 759, 810
Sse9I AATT 6 cut(s) 313, 392, 664, 897, 1035, 1070
SseBI AGGCCT 1 cut(s) 561
SsiI CCGC 1 cut(s) 1076
SspI AATATT 1 cut(s) 796
SspMI CTAG 2 cut(s) 329, 647
StuI AGGCCT 1 cut(s) 561
StyD4I CCNGG 4 cut(s) 290, 502, 1017, 1039
StyI CCWWGG 1 cut(s) 407
TaaI ACNGT 3 cut(s) 486, 892, 977
TaqI TCGA 1 cut(s) 811
TaqII GACCGA 1 cut(s) 455
TasI AATT 6 cut(s) 313, 392, 664, 897, 1035, 1070
TatI WGTACW 1 cut(s) 972
TfiI GAWTC 2 cut(s) 449, 951
Tru1I TTAA 4 cut(s) 705, 945, 1001, 1143
Tru9I TTAA 4 cut(s) 705, 945, 1001, 1143
TscAI CASTG 2 cut(s) 25, 503
TseFI GTSAC 4 cut(s) 41, 133, 167, 716
TseI GCWGC 3 cut(s) 218, 398, 928
Tsp45I GTSAC 4 cut(s) 41, 133, 167, 716
TspDTI ATGAA 3 cut(s) 227, 534, 1122
TspRI CASTG 2 cut(s) 25, 503
Tth111I GACNNNGTC 1 cut(s) 1025
Van91I CCANNNNNTGG 1 cut(s) 180
VneI GTGCAC 1 cut(s) 1080
XagI CCTNNNNNAGG 1 cut(s) 566
XapI RAATTY 3 cut(s) 313, 392, 1070
XceI RCATGY 1 cut(s) 14
XhoI CTCGAG 1 cut(s) 810
XmiI GTMKAC 1 cut(s) 782
XmnI GAANNNNTTC 1 cut(s) 795
XspI CTAG 2 cut(s) 329, 647
Zsp2I ATGCAT 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.