MD17G1132700.v1.1

oxidoreductase At1g06690, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005042
Physical Location & Seq
Reverse (-)
11843191 .. 11845894
2704 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1132700.v1.1.491

Sequence Viewer

Length: 957 bp
ATGGCTATGCATGTCAGCAATGCATGTTTTTCTGCTTTGAGTGAGAGGAGAGTTCTTAGGGTTAGAGCTGTTGCTTCTGAGAACGTGACCACAGAGGATGATAAGGTGAAATTGGGTGGCTCTGATTTGAAGGTGACAAGGCTTGGGATTGAGCTTCCAGTGGGATGGAAAATGAAAGTGGCCAAAGCTGCTTTCGATGTCAATATTGATGGTGGAATCACCTTCTTTGATACTGCTGAAGTGTGCGGCTCCAGGGCTTCTTTTGGTGCTGTAAATTCTGAAACTTTACTAGGAAGATTTATCAAGGAAAAGAAACAGAAGGATCCAAGAGTAGACGTTGCTGTCGCAACCAAATTCGCAGCTTTGCTGTGGAGGCTAGGTCGTCAAAGTGCCATAGCTGCCCTCAAGGATTCCCTCAATTGCCTTGAACTTTCTTCGGTGTATTTGGGAAATCAATTTTCAGGGTATCTTGATGGTCTTGGAGATGCTGTTGAGCAGGGCCTTGTGAAGGCTGTTGGTGTTTCCAACTACAGCCAAAAGCAATTGCGTAAGGCACACGCAAAACTTCAAAATAGAGGTATCCCACCAGCATCAAATCAGGTGAATTACAGCCTCATATACAAGGCACCAAAGGAGAATGGTGTGAAGTTTACCTGTGATGAACTTGGGATCACTTTGATTGCCTATTCGCCTATTGCTCAAGGTGAACTCCAACCTCTGCTGAAGCGGATCAAGGCAATAGGAGAAAAGTACAGTAAAATAAACACACAGGTAGTCCTAAACTGGTTGATAGCCCAGGGCAACGTTGTGCCAATCCCGGGAGCCAAAAAAGCAGAACAGGTTGCAGAATTTGCAAGTTCACTTGGATGGCGGCTCAACACTGAAGAAGTAGCCGAATTGCGCGCTCTGGTTATAGAGATAAAACCTGTTACCGGTTTCCCTGTTGAAAAATTATAA

Protein Analysis

319

Amino Acids

34.55

Weight (kDa)

9.35

Isoelectric Point (pI)

36.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 63 - 303 8.1e-33 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 955
AasI GACNNNNNNGTC 1 cut(s) 341
AccB1I GGYRCC 1 cut(s) 625
AccI GTMKAC 1 cut(s) 333
AccII CGCG 1 cut(s) 903
AciI CCGC 3 cut(s) 246, 727, 871
AclI AACGTT 1 cut(s) 804
AclWI GGATC 4 cut(s) 317, 330, 677, 737
AcoI YGGCCR 1 cut(s) 180
AcsI RAATTY 3 cut(s) 274, 353, 848
AcuI CTGAAG 3 cut(s) 258, 743, 903
AfaI GTAC 1 cut(s) 752
AfiI CCNNNNNNNGG 3 cut(s) 508, 818, 932
AgeI ACCGGT 1 cut(s) 932
AgsI TTSAA 4 cut(s) 130, 428, 569, 947
AjnI CCWGG 2 cut(s) 251, 795
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
AluBI AGCT 5 cut(s) 68, 154, 188, 362, 398
AluI AGCT 5 cut(s) 68, 154, 188, 362, 398
AlwI GGATC 4 cut(s) 317, 330, 677, 737
Ama87I CYCGRG 1 cut(s) 817
AoxI GGCC 2 cut(s) 180, 499
ApeKI GCWGC 3 cut(s) 188, 359, 398
ApoI RAATTY 3 cut(s) 274, 353, 848
AsiGI ACCGGT 1 cut(s) 932
AspLEI GCGC 2 cut(s) 903, 905
AspS9I GGNCC 1 cut(s) 499
AsuC2I CCSGG 2 cut(s) 818, 819
AsuHPI GGTGA 5 cut(s) 118, 145, 211, 613, 716
AvaI CYCGRG 1 cut(s) 817
BalI TGGCCA 1 cut(s) 182
BamHI GGATCC 1 cut(s) 322
BanI GGYRCC 1 cut(s) 625
BbvI GCAGC 3 cut(s) 175, 371, 385
BccI CCATC 4 cut(s) 159, 203, 467, 861
BciT130I CCWGG 2 cut(s) 253, 797
BciVI GTATCC 1 cut(s) 590
BcnI CCSGG 2 cut(s) 818, 819
BfaI CTAG 2 cut(s) 290, 377
BfmI CTRYAG 1 cut(s) 529
BfuI GTATCC 1 cut(s) 590
BisI GCNGC 5 cut(s) 189, 247, 360, 399, 872
BlsI GCNGC 5 cut(s) 190, 248, 361, 400, 873
Bme1390I CCNGG 4 cut(s) 253, 797, 818, 819
BmeT110I CYCGRG 1 cut(s) 817
BmgT120I GGNCC 1 cut(s) 499
BmiI GGNNCC 4 cut(s) 250, 324, 627, 823
BmrFI CCNGG 4 cut(s) 253, 797, 818, 819
BmsI GCATC 2 cut(s) 475, 599
BpmI CTGGAG 1 cut(s) 235
BpuEI CTTGAG 2 cut(s) 389, 684
BpuMI CCSGG 2 cut(s) 818, 819
BsaJI CCNNGG 4 cut(s) 252, 795, 796, 817
BsaWI WCCGGW 1 cut(s) 932
BsaXI ACNNNNNCTCC 4 cut(s) 364, 394, 474, 504
Bsc4I CCNNNNNNNGG 3 cut(s) 508, 818, 932
Bse118I RCCGGY 1 cut(s) 932
Bse1I ACTGG 2 cut(s) 158, 788
Bse3DI GCAATG 1 cut(s) 25
BseBI CCWGG 2 cut(s) 253, 797
BseDI CCNNGG 4 cut(s) 252, 795, 796, 817
BseGI GGATG 3 cut(s) 103, 170, 872
BseLI CCNNNNNNNGG 3 cut(s) 508, 818, 932
BseMI GCAATG 1 cut(s) 25
BseMII CTCAG 1 cut(s) 69
BseNI ACTGG 2 cut(s) 158, 788
BsePI GCGCGC 1 cut(s) 901
BseRI GAGGAG 1 cut(s) 61
BseXI GCAGC 3 cut(s) 175, 371, 385
Bsh1236I CGCG 1 cut(s) 903
BshFI GGCC 2 cut(s) 182, 501
BshNI GGYRCC 1 cut(s) 625
BshTI ACCGGT 1 cut(s) 932
BsiHKCI CYCGRG 1 cut(s) 817
BsiSI CCGG 2 cut(s) 818, 933
BslI CCNNNNNNNGG 3 cut(s) 508, 818, 932
BsnI GGCC 2 cut(s) 182, 501
BsoBI CYCGRG 1 cut(s) 817
Bsp143I GATC 3 cut(s) 322, 669, 729
BspACI CCGC 3 cut(s) 246, 727, 871
BspANI GGCC 2 cut(s) 182, 501
BspCNI CTCAG 1 cut(s) 70
BspFNI CGCG 1 cut(s) 903
BspLI GGNNCC 4 cut(s) 250, 324, 627, 823
BspPI GGATC 4 cut(s) 317, 330, 677, 737
BspT107I GGYRCC 1 cut(s) 625
BsrDI GCAATG 1 cut(s) 25
BsrFI RCCGGY 1 cut(s) 932
BsrI ACTGG 2 cut(s) 158, 788
BssAI RCCGGY 1 cut(s) 932
BssECI CCNNGG 4 cut(s) 252, 795, 796, 817
BssHII GCGCGC 1 cut(s) 901
BssMI GATC 3 cut(s) 322, 669, 729
Bst2UI CCWGG 2 cut(s) 253, 797
Bst4CI ACNGT 1 cut(s) 755
BstAPI GCANNNNNTGC 1 cut(s) 851
BstC8I GCNNGC 1 cut(s) 903
BstDEI CTNAG 2 cut(s) 56, 78
BstENI CCTNNNNNAGG 1 cut(s) 506
BstF5I GGATG 3 cut(s) 103, 170, 872
BstFNI CGCG 1 cut(s) 903
BstHHI GCGC 2 cut(s) 903, 905
BstKTI GATC 3 cut(s) 325, 672, 732
BstMBI GATC 3 cut(s) 322, 669, 729
BstMWI GCNNNNNNNGC 5 cut(s) 188, 373, 398, 830, 851
BstNI CCWGG 2 cut(s) 253, 797
BstNSI RCATGY 2 cut(s) 14, 27
BstSCI CCNGG 4 cut(s) 251, 795, 816, 817
BstSFI CTRYAG 1 cut(s) 529
BstUI CGCG 1 cut(s) 903
BstV1I GCAGC 3 cut(s) 175, 371, 385
BstX2I RGATCY 1 cut(s) 322
BstXI CCANNNNNNTGG 1 cut(s) 165
BstYI RGATCY 1 cut(s) 322
BsuI GTATCC 1 cut(s) 590
BsuRI GGCC 2 cut(s) 182, 501
BtsCI GGATG 3 cut(s) 103, 170, 872
BtsIMutI CAGTG 2 cut(s) 165, 879
Cac8I GCNNGC 1 cut(s) 903
CfoI GCGC 2 cut(s) 903, 905
Cfr10I RCCGGY 1 cut(s) 932
Cfr13I GGNCC 1 cut(s) 499
Cfr9I CCCGGG 1 cut(s) 817
Csp6I GTAC 1 cut(s) 751
CspAI ACCGGT 1 cut(s) 932
CviAII CATG 2 cut(s) 11, 24
CviQI GTAC 1 cut(s) 751
DdeI CTNAG 2 cut(s) 56, 78
DpnI GATC 3 cut(s) 324, 671, 731
DpnII GATC 3 cut(s) 322, 669, 729
DrdI GACNNNNNNGTC 1 cut(s) 341
DseDI GACNNNNNNGTC 1 cut(s) 341
EaeI YGGCCR 1 cut(s) 180
Eco57I CTGAAG 3 cut(s) 258, 743, 903
Eco88I CYCGRG 1 cut(s) 817
EcoNI CCTNNNNNAGG 1 cut(s) 506
EcoO109I RGGNCCY 1 cut(s) 499
EcoRII CCWGG 2 cut(s) 251, 795
EcoT22I ATGCAT 2 cut(s) 12, 25
FaeI CATG 2 cut(s) 14, 27
FaiI YATR 8 cut(s) 8, 12, 25, 395, 617, 619, 914, 955
FatI CATG 2 cut(s) 10, 23
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 5 cut(s) 189, 247, 360, 399, 872
FokI GGATG 3 cut(s) 110, 177, 879
Fsp4HI GCNGC 5 cut(s) 189, 247, 360, 399, 872
FspBI CTAG 2 cut(s) 290, 377
GlaI GCGC 2 cut(s) 902, 904
GluI GCNGC 5 cut(s) 189, 247, 360, 399, 872
GsuI CTGGAG 1 cut(s) 235
HaeIII GGCC 2 cut(s) 182, 501
HapII CCGG 2 cut(s) 818, 933
HhaI GCGC 2 cut(s) 903, 905
Hin1II CATG 2 cut(s) 14, 27
Hin6I GCGC 2 cut(s) 901, 903
HinP1I GCGC 2 cut(s) 901, 903
HinfI GANTC 2 cut(s) 216, 410
HpaII CCGG 2 cut(s) 818, 933
HphI GGTGA 5 cut(s) 118, 145, 211, 613, 716
Hpy166II GTNNAC 4 cut(s) 334, 651, 707, 860
Hpy188I TCNGA 3 cut(s) 79, 124, 280
Hpy188III TCNNGA 1 cut(s) 470
Hpy8I GTNNAC 4 cut(s) 334, 651, 707, 860
HpyAV CCTTC 4 cut(s) 124, 232, 313, 502
HpyCH4III ACNGT 1 cut(s) 755
HpyCH4IV ACGT 3 cut(s) 84, 336, 804
HpyCH4V TGCA 4 cut(s) 10, 23, 845, 854
HpyF10VI GCNNNNNNNGC 5 cut(s) 188, 373, 398, 830, 851
HpyF3I CTNAG 2 cut(s) 56, 78
HpySE526I ACGT 3 cut(s) 84, 336, 804
Hsp92II CATG 2 cut(s) 14, 27
HspAI GCGC 2 cut(s) 901, 903
Kzo9I GATC 3 cut(s) 322, 669, 729
LmnI GCTCC 2 cut(s) 254, 821
Lsp1109I GCAGC 3 cut(s) 175, 371, 385
LweI GCATC 2 cut(s) 475, 599
MaeI CTAG 2 cut(s) 290, 377
MaeII ACGT 3 cut(s) 84, 336, 804
MaeIII GTNAC 3 cut(s) 85, 133, 928
MalI GATC 3 cut(s) 324, 671, 731
MboI GATC 3 cut(s) 322, 669, 729
MboII GAAGA 3 cut(s) 306, 426, 896
MfeI CAATTG 2 cut(s) 418, 542
MflI RGATCY 1 cut(s) 322
MlsI TGGCCA 1 cut(s) 182
MluNI TGGCCA 1 cut(s) 182
MmeI TCCRAC 2 cut(s) 549, 736
MnlI CCTC 8 cut(s) 39, 88, 366, 413, 425, 569, 623, 726
Mox20I TGGCCA 1 cut(s) 182
Mph1103I ATGCAT 2 cut(s) 12, 25
MscI TGGCCA 1 cut(s) 182
MslI CAYNNNNRTG 1 cut(s) 865
Msp20I TGGCCA 1 cut(s) 182
MspI CCGG 2 cut(s) 818, 933
MspR9I CCNGG 4 cut(s) 253, 797, 818, 819
MunI CAATTG 2 cut(s) 418, 542
MvaI CCWGG 2 cut(s) 253, 797
MvnI CGCG 1 cut(s) 903
MwoI GCNNNNNNNGC 5 cut(s) 188, 373, 398, 830, 851
NciI CCSGG 2 cut(s) 818, 819
NdeII GATC 3 cut(s) 322, 669, 729
NlaIII CATG 2 cut(s) 14, 27
NlaIV GGNNCC 4 cut(s) 250, 324, 627, 823
NmuCI GTSAC 2 cut(s) 85, 133
NsiI ATGCAT 2 cut(s) 12, 25
NspI RCATGY 2 cut(s) 14, 27
PasI CCCWGGG 1 cut(s) 796
PauI GCGCGC 1 cut(s) 901
PfeI GAWTC 2 cut(s) 216, 410
PinAI ACCGGT 1 cut(s) 932
PkrI GCNGC 5 cut(s) 190, 248, 361, 400, 873
PsiI TTATAA 1 cut(s) 955
Psp1406I AACGTT 1 cut(s) 804
Psp6I CCWGG 2 cut(s) 251, 795
PspGI CCWGG 2 cut(s) 251, 795
PspN4I GGNNCC 4 cut(s) 250, 324, 627, 823
PspPI GGNCC 1 cut(s) 499
PsuI RGATCY 1 cut(s) 322
PteI GCGCGC 1 cut(s) 901
RsaI GTAC 1 cut(s) 752
RsaNI GTAC 1 cut(s) 751
RseI CAYNNNNRTG 1 cut(s) 865
SatI GCNGC 5 cut(s) 189, 247, 360, 399, 872
Sau3AI GATC 3 cut(s) 322, 669, 729
Sau96I GGNCC 1 cut(s) 499
ScrFI CCNGG 4 cut(s) 253, 797, 818, 819
SfaNI GCATC 2 cut(s) 475, 599
SfcI CTRYAG 1 cut(s) 529
SmaI CCCGGG 1 cut(s) 819
SmiMI CAYNNNNRTG 1 cut(s) 865
SmlI CTYRAG 2 cut(s) 404, 699
SmoI CTYRAG 2 cut(s) 404, 699
SsiI CCGC 3 cut(s) 246, 727, 871
SspI AATATT 1 cut(s) 205
SspMI CTAG 2 cut(s) 290, 377
StyD4I CCNGG 4 cut(s) 251, 795, 816, 817
TaaI ACNGT 1 cut(s) 755
TaiI ACGT 3 cut(s) 87, 339, 807
TaqI TCGA 1 cut(s) 195
TatI WGTACW 1 cut(s) 750
TauI GCSGC 2 cut(s) 249, 874
TfiI GAWTC 2 cut(s) 216, 410
TscAI CASTG 2 cut(s) 165, 886
TseFI GTSAC 2 cut(s) 85, 133
TseI GCWGC 3 cut(s) 188, 359, 398
Tsp45I GTSAC 2 cut(s) 85, 133
TspDTI ATGAA 2 cut(s) 188, 675
TspMI CCCGGG 1 cut(s) 817
TspRI CASTG 2 cut(s) 165, 886
XagI CCTNNNNNAGG 1 cut(s) 506
XapI RAATTY 3 cut(s) 274, 353, 848
XceI RCATGY 2 cut(s) 14, 27
XmaI CCCGGG 1 cut(s) 817
XmiI GTMKAC 1 cut(s) 333
XspI CTAG 2 cut(s) 290, 377
Zsp2I ATGCAT 2 cut(s) 12, 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.