MD09G1275000.v1.1

gal1,galk

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005927
Physical Location & Seq
Forward (+)
35230902 .. 35238343
7442 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1275000.v1.1.491

Sequence Viewer

Length: 651 bp
ATGACACAAATGGCAAAACACGAAGAACTCCCGATCCCAATTCACAACACCCTCGACGCCGTCTACGGCGACTCCTCCCAACTCGACGAGGCCCAGCTCCGGTTCCACCAGCTGAAGTCCAAGTTCCAGCAGGTGGGTCACCCTCCCCATCTCTTCGCTCGCTCTTCAGGGAGAGTAAACTTGATCGGAGAGCACATAGACTACGAGCGCTACTCGATGCTGCCGATGGCCATACGACAGGACACCGTTGTCGCAATCCGGAAGCACGACGACGGAGATGCCGCGAAAGTTCTCAGAATCGCCAACGTCAACGACAAGTACCAAATTTGTACTTATCCCGCCGACCCGACCCAGGAAATTGACTTGAAGAATCACATAGGGGCACTATTTCATTTGTGGCTTACTAAGCCATATGCAGCTGATTCAACACCTGGGCAAGATGACTGTGCAGATGAGAAACCTTCCGTTAAAATCCCGGAAATTGCGGGGAAGGTAGAGAGTGGTATAAGAAGTCCGGGCTTGCAGTGTATGTCGCCTGATTATGACACAAAGGAAATAAAAAACATATATGTGGATTGTAAGCTTTCTTCTTTTCCTATTTTGTTATCGTTTTATTTAAGTTTGGCGTTCTCCTTCCATCCTTACTTTTGA

Protein Analysis

217

Amino Acids

24.42

Weight (kDa)

5.9

Isoelectric Point (pI)

49.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GalKase_gal_bdg PF10509 44 - 88 6.6e-18 Galactokinase galactose-binding signature
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 121
AasI GACNNNNNNGTC 1 cut(s) 248
Acc36I ACCTGC 1 cut(s) 121
AccB7I CCANNNNNTGG 1 cut(s) 133
AccI GTMKAC 1 cut(s) 63
AccII CGCG 1 cut(s) 284
AccIII TCCGGA 1 cut(s) 258
AciI CCGC 3 cut(s) 282, 339, 485
AclWI GGATC 1 cut(s) 28
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 1 cut(s) 324
AcuI CTGAAG 2 cut(s) 134, 150
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 2 cut(s) 320, 331
AfeI AGCGCT 1 cut(s) 209
AfiI CCNNNNNNNGG 3 cut(s) 99, 133, 352
AgsI TTSAA 2 cut(s) 367, 426
AjnI CCWGG 2 cut(s) 351, 430
AloI GAACNNNNNNTCC 2 cut(s) 18, 50
AluBI AGCT 4 cut(s) 97, 112, 419, 583
AluI AGCT 4 cut(s) 97, 112, 419, 583
Alw21I GWGCWC 1 cut(s) 195
AlwI GGATC 1 cut(s) 28
Aor13HI TCCGGA 1 cut(s) 258
Aor51HI AGCGCT 1 cut(s) 209
AoxI GGCC 2 cut(s) 90, 228
ApeKI GCWGC 2 cut(s) 220, 416
ApoI RAATTY 1 cut(s) 324
AspLEI GCGC 1 cut(s) 210
AspS9I GGNCC 1 cut(s) 91
AsuC2I CCSGG 2 cut(s) 476, 516
AsuHPI GGTGA 1 cut(s) 131
BaeGI GKGCMC 1 cut(s) 385
BalI TGGCCA 1 cut(s) 230
BarI GAAGNNNNNNTAC 2 cut(s) 571, 603
Bbv12I GWGCWC 1 cut(s) 195
BbvI GCAGC 2 cut(s) 207, 428
BccI CCATC 3 cut(s) 156, 220, 645
BceAI ACGGC 2 cut(s) 44, 82
BcgI CGANNNNNNTGC 2 cut(s) 260, 294
BciT130I CCWGG 2 cut(s) 353, 432
BcnI CCSGG 2 cut(s) 476, 516
BfoI RGCGCY 1 cut(s) 211
BfuAI ACCTGC 1 cut(s) 121
BisI GCNGC 3 cut(s) 221, 282, 417
BlsI GCNGC 3 cut(s) 222, 283, 418
Bme1390I CCNGG 4 cut(s) 353, 432, 476, 516
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 1 cut(s) 104
BmrFI CCNGG 4 cut(s) 353, 432, 476, 516
BmsI GCATC 2 cut(s) 207, 268
BplI GAGNNNNNCTC 2 cut(s) 197, 229
BpuMI CCSGG 2 cut(s) 476, 516
BsaHI GRCGYC 1 cut(s) 57
BsaJI CCNNGG 2 cut(s) 351, 431
BsaWI WCCGGW 2 cut(s) 99, 258
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 3 cut(s) 99, 133, 352
BseAI TCCGGA 1 cut(s) 258
BseBI CCWGG 2 cut(s) 353, 432
BseDI CCNNGG 2 cut(s) 351, 431
BseGI GGATG 1 cut(s) 637
BseLI CCNNNNNNNGG 3 cut(s) 99, 133, 352
BseMII CTCAG 1 cut(s) 307
BseRI GAGGAG 1 cut(s) 64
BseSI GKGCMC 1 cut(s) 385
BseXI GCAGC 2 cut(s) 207, 428
BseYI CCCAGC 1 cut(s) 93
BsgI GTGCAG 1 cut(s) 468
Bsh1236I CGCG 1 cut(s) 284
BshFI GGCC 2 cut(s) 92, 230
BsiHKAI GWGCWC 1 cut(s) 195
BsiSI CCGG 4 cut(s) 100, 259, 476, 515
BslI CCNNNNNNNGG 3 cut(s) 99, 133, 352
BsnI GGCC 2 cut(s) 92, 230
Bsp1286I GDGCHC 2 cut(s) 195, 385
Bsp13I TCCGGA 1 cut(s) 258
Bsp143I GATC 2 cut(s) 33, 183
BspACI CCGC 3 cut(s) 282, 339, 485
BspANI GGCC 2 cut(s) 92, 230
BspCNI CTCAG 1 cut(s) 306
BspEI TCCGGA 1 cut(s) 258
BspFNI CGCG 1 cut(s) 284
BspLI GGNNCC 1 cut(s) 104
BspMI ACCTGC 1 cut(s) 121
BspPI GGATC 1 cut(s) 28
BspQI GCTCTTC 1 cut(s) 169
BssECI CCNNGG 2 cut(s) 351, 431
BssMI GATC 2 cut(s) 33, 183
BssNI GRCGYC 1 cut(s) 57
Bst2UI CCWGG 2 cut(s) 353, 432
Bst4CI ACNGT 2 cut(s) 247, 446
Bst6I CTCTTC 2 cut(s) 158, 169
BstACI GRCGYC 1 cut(s) 57
BstC8I GCNNGC 2 cut(s) 160, 521
BstDEI CTNAG 2 cut(s) 293, 405
BstEII GGTNACC 1 cut(s) 137
BstF5I GGATG 1 cut(s) 637
BstFNI CGCG 1 cut(s) 284
BstH2I RGCGCY 1 cut(s) 211
BstHHI GCGC 1 cut(s) 210
BstKTI GATC 2 cut(s) 36, 186
BstMBI GATC 2 cut(s) 33, 183
BstMWI GCNNNNNNNGC 1 cut(s) 406
BstNI CCWGG 2 cut(s) 353, 432
BstPI GGTNACC 1 cut(s) 137
BstSCI CCNGG 4 cut(s) 351, 430, 474, 514
BstSLI GKGCMC 1 cut(s) 385
BstUI CGCG 1 cut(s) 284
BstV1I GCAGC 2 cut(s) 207, 428
BsuRI GGCC 2 cut(s) 92, 230
BtsCI GGATG 1 cut(s) 637
BtsI GCAGTG 1 cut(s) 530
BtsIMutI CAGTG 1 cut(s) 530
BveI ACCTGC 1 cut(s) 121
Cac8I GCNNGC 2 cut(s) 160, 521
CfoI GCGC 1 cut(s) 210
Cfr13I GGNCC 1 cut(s) 91
CseI GACGC 1 cut(s) 65
Csp6I GTAC 2 cut(s) 319, 330
CviJI RGCY 9 cut(s) 92, 97, 112, 230, 400, 409, 419, 519, 583
CviKI_1 RGCY 9 cut(s) 92, 97, 112, 230, 400, 409, 419, 519, 583
CviQI GTAC 2 cut(s) 319, 330
DdeI CTNAG 2 cut(s) 293, 405
DpnI GATC 2 cut(s) 35, 185
DpnII GATC 2 cut(s) 33, 183
DrdI GACNNNNNNGTC 1 cut(s) 248
DseDI GACNNNNNNGTC 1 cut(s) 248
EaeI YGGCCR 1 cut(s) 228
Eam1104I CTCTTC 2 cut(s) 158, 169
EarI CTCTTC 2 cut(s) 158, 169
Eco47III AGCGCT 1 cut(s) 209
Eco57I CTGAAG 2 cut(s) 134, 150
Eco91I GGTNACC 1 cut(s) 137
EcoO65I GGTNACC 1 cut(s) 137
EcoRII CCWGG 2 cut(s) 351, 430
FauI CCCGC 2 cut(s) 346, 478
FauNDI CATATG 1 cut(s) 412
FblI GTMKAC 1 cut(s) 63
Fnu4HI GCNGC 3 cut(s) 221, 282, 417
FokI GGATG 1 cut(s) 624
Fsp4HI GCNGC 3 cut(s) 221, 282, 417
GlaI GCGC 1 cut(s) 209
GluI GCNGC 3 cut(s) 221, 282, 417
GsaI CCCAGC 1 cut(s) 97
HaeII RGCGCY 1 cut(s) 211
HaeIII GGCC 2 cut(s) 92, 230
HapII CCGG 4 cut(s) 100, 259, 476, 515
HgaI GACGC 1 cut(s) 65
HhaI GCGC 1 cut(s) 210
Hin1I GRCGYC 1 cut(s) 57
Hin6I GCGC 1 cut(s) 208
HinP1I GCGC 1 cut(s) 208
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HindIII AAGCTT 1 cut(s) 581
HinfI GANTC 4 cut(s) 71, 297, 370, 422
HpaII CCGG 4 cut(s) 100, 259, 476, 515
HphI GGTGA 1 cut(s) 131
Hpy166II GTNNAC 3 cut(s) 64, 178, 310
Hpy188I TCNGA 2 cut(s) 188, 296
Hpy188III TCNNGA 2 cut(s) 31, 259
Hpy8I GTNNAC 3 cut(s) 64, 178, 310
Hpy99I CGWCG 4 cut(s) 59, 89, 272, 275
HpyAV CCTTC 3 cut(s) 471, 484, 643
HpyCH4III ACNGT 2 cut(s) 247, 446
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 3 cut(s) 416, 449, 523
HpyF10VI GCNNNNNNNGC 1 cut(s) 406
HpyF3I CTNAG 2 cut(s) 293, 405
HpySE526I ACGT 1 cut(s) 306
Hsp92I GRCGYC 1 cut(s) 57
HspAI GCGC 1 cut(s) 208
Kpn2I TCCGGA 1 cut(s) 258
Kzo9I GATC 2 cut(s) 33, 183
LguI GCTCTTC 1 cut(s) 169
LmnI GCTCC 1 cut(s) 102
Lsp1109I GCAGC 2 cut(s) 207, 428
LweI GCATC 2 cut(s) 207, 268
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 1 cut(s) 137
MalI GATC 2 cut(s) 35, 185
MboI GATC 2 cut(s) 33, 183
MboII GAAGA 5 cut(s) 35, 145, 156, 379, 579
MhlI GDGCHC 2 cut(s) 195, 385
MlsI TGGCCA 1 cut(s) 230
MluCI AATT 4 cut(s) 39, 324, 357, 480
MluNI TGGCCA 1 cut(s) 230
MlyI GAGTC 1 cut(s) 65
MnlI CCTC 4 cut(s) 62, 82, 85, 153
Mox20I TGGCCA 1 cut(s) 230
MroI TCCGGA 1 cut(s) 258
MscI TGGCCA 1 cut(s) 230
MseI TTAA 2 cut(s) 468, 617
MslI CAYNNNNRTG 1 cut(s) 569
Msp20I TGGCCA 1 cut(s) 230
MspA1I CMGCKG 2 cut(s) 112, 419
MspI CCGG 4 cut(s) 100, 259, 476, 515
MspR9I CCNGG 4 cut(s) 353, 432, 476, 516
MvaI CCWGG 2 cut(s) 353, 432
MvnI CGCG 1 cut(s) 284
MwoI GCNNNNNNNGC 1 cut(s) 406
NciI CCSGG 2 cut(s) 476, 516
NdeI CATATG 1 cut(s) 412
NdeII GATC 2 cut(s) 33, 183
NlaIV GGNNCC 1 cut(s) 104
NmuCI GTSAC 1 cut(s) 137
PaqCI CACCTGC 1 cut(s) 121
PciSI GCTCTTC 1 cut(s) 169
PcsI WCGNNNNNNNCGW 1 cut(s) 221
PfeI GAWTC 3 cut(s) 297, 370, 422
PflFI GACNNNGTC 1 cut(s) 59
PflMI CCANNNNNTGG 1 cut(s) 133
PfoI TCCNGGA 1 cut(s) 474
PkrI GCNGC 3 cut(s) 222, 283, 418
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
Psp6I CCWGG 2 cut(s) 351, 430
PspEI GGTNACC 1 cut(s) 137
PspFI CCCAGC 1 cut(s) 93
PspGI CCWGG 2 cut(s) 351, 430
PspN4I GGNNCC 1 cut(s) 104
PspPI GGNCC 1 cut(s) 91
PsyI GACNNNGTC 1 cut(s) 59
PvuII CAGCTG 2 cut(s) 112, 419
RsaI GTAC 2 cut(s) 320, 331
RsaNI GTAC 2 cut(s) 319, 330
RseI CAYNNNNRTG 1 cut(s) 569
SapI GCTCTTC 1 cut(s) 169
SaqAI TTAA 2 cut(s) 468, 617
SatI GCNGC 3 cut(s) 221, 282, 417
Sau3AI GATC 2 cut(s) 33, 183
Sau96I GGNCC 1 cut(s) 91
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 4 cut(s) 353, 432, 476, 516
SduI GDGCHC 2 cut(s) 195, 385
SetI ASST 9 cut(s) 99, 114, 135, 309, 421, 433, 463, 495, 585
SfaNI GCATC 2 cut(s) 207, 268
SmiMI CAYNNNNRTG 1 cut(s) 569
Sse9I AATT 4 cut(s) 39, 324, 357, 480
SsiI CCGC 3 cut(s) 282, 339, 485
StyD4I CCNGG 4 cut(s) 351, 430, 474, 514
TaaI ACNGT 2 cut(s) 247, 446
TaiI ACGT 1 cut(s) 309
TaqI TCGA 3 cut(s) 54, 84, 215
TasI AATT 4 cut(s) 39, 324, 357, 480
TatI WGTACW 1 cut(s) 329
TauI GCSGC 1 cut(s) 284
TfiI GAWTC 3 cut(s) 297, 370, 422
Tru1I TTAA 2 cut(s) 468, 617
Tru9I TTAA 2 cut(s) 468, 617
TscAI CASTG 1 cut(s) 530
TseFI GTSAC 1 cut(s) 137
TseI GCWGC 2 cut(s) 220, 416
Tsp45I GTSAC 1 cut(s) 137
TspDTI ATGAA 1 cut(s) 380
TspGWI ACGGA 2 cut(s) 288, 454
TspRI CASTG 1 cut(s) 530
Tth111I GACNNNGTC 1 cut(s) 59
Van91I CCANNNNNTGG 1 cut(s) 133
XapI RAATTY 1 cut(s) 324
XmiI GTMKAC 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.