Rh5CG262600

gal1,galk

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0005927
Physical Location & Seq
Reverse (-)
28032356 .. 28038087
5732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG262600.1

Sequence Viewer

Length: 267 bp
ATGGCTCAAATGATGAGCCACGAAGAACTCCCGATCCGAATCCACTTCGCTGTCGACGAAGTCTACGACGATCCCTCGCAACTCGAGGAGGCCCAGCTCCGCTTACACCGCCTCAAGACCAAGTTTCACAAGATCTTCGGGCACCTTCCTCAAGTCTGCGCTCGCTCTCCAGGGAGAGTGAATTTGATTGGAGAGCATACAGACTATGATGGATTCTCGGTGTTGCCGATGCCAATACGGCAGGATATATACTATTGTGGCGATTAG

Protein Analysis

88

Amino Acids

10.25

Weight (kDa)

5.8

Isoelectric Point (pI)

58.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GalKase_gal_bdg PF10509 41 - 85 2.5e-20 Galactokinase galactose-binding signature
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 141
AccI GTMKAC 2 cut(s) 54, 63
AciI CCGC 2 cut(s) 100, 109
AclWI GGATC 2 cut(s) 28, 65
AcsI RAATTY 1 cut(s) 181
AjnI CCWGG 1 cut(s) 169
AloI GAACNNNNNNTCC 2 cut(s) 18, 50
AluBI AGCT 1 cut(s) 97
AluI AGCT 1 cut(s) 97
AlwI GGATC 2 cut(s) 28, 65
Ama87I CYCGRG 1 cut(s) 83
AoxI GGCC 1 cut(s) 90
ApoI RAATTY 1 cut(s) 181
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 91
AvaI CYCGRG 1 cut(s) 83
BaeGI GKGCMC 1 cut(s) 144
BanI GGYRCC 1 cut(s) 141
BccI CCATC 1 cut(s) 203
BceAI ACGGC 1 cut(s) 254
BciT130I CCWGG 1 cut(s) 171
BglI GCCNNNNNGGC 1 cut(s) 238
BglII AGATCT 1 cut(s) 132
Bme1390I CCNGG 1 cut(s) 171
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 1 cut(s) 143
BmrFI CCNGG 1 cut(s) 171
BmsI GCATC 1 cut(s) 219
BpmI CTGGAG 1 cut(s) 153
BpuEI CTTGAG 2 cut(s) 98, 135
BsaBI GATNNNNATC 1 cut(s) 38
BsaJI CCNNGG 1 cut(s) 170
Bse8I GATNNNNATC 1 cut(s) 38
BseBI CCWGG 1 cut(s) 171
BseDI CCNNGG 1 cut(s) 170
BseJI GATNNNNATC 1 cut(s) 38
BseRI GAGGAG 1 cut(s) 101
BseSI GKGCMC 1 cut(s) 144
BseYI CCCAGC 1 cut(s) 93
BshFI GGCC 1 cut(s) 92
BshNI GGYRCC 1 cut(s) 141
BsiHKCI CYCGRG 1 cut(s) 83
BsnI GGCC 1 cut(s) 92
BsoBI CYCGRG 1 cut(s) 83
Bsp1286I GDGCHC 1 cut(s) 144
Bsp143I GATC 3 cut(s) 33, 70, 132
BspACI CCGC 2 cut(s) 100, 109
BspANI GGCC 1 cut(s) 92
BspLI GGNNCC 1 cut(s) 143
BspPI GGATC 2 cut(s) 28, 65
BspT107I GGYRCC 1 cut(s) 141
BssECI CCNNGG 1 cut(s) 170
BssMI GATC 3 cut(s) 33, 70, 132
Bst2UI CCWGG 1 cut(s) 171
BstC8I GCNNGC 1 cut(s) 163
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 3 cut(s) 36, 73, 135
BstMBI GATC 3 cut(s) 33, 70, 132
BstMWI GCNNNNNNNGC 2 cut(s) 108, 238
BstNI CCWGG 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 169
BstSLI GKGCMC 1 cut(s) 144
BstX2I RGATCY 1 cut(s) 132
BstYI RGATCY 1 cut(s) 132
BsuRI GGCC 1 cut(s) 92
Cac8I GCNNGC 1 cut(s) 163
CfoI GCGC 1 cut(s) 161
Cfr13I GGNCC 1 cut(s) 91
CviJI RGCY 4 cut(s) 5, 18, 92, 97
CviKI_1 RGCY 4 cut(s) 5, 18, 92, 97
DpnI GATC 3 cut(s) 35, 72, 134
DpnII GATC 3 cut(s) 33, 70, 132
Eco88I CYCGRG 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 169
FaiI YATR 4 cut(s) 198, 207, 248, 250
FblI GTMKAC 2 cut(s) 54, 63
GlaI GCGC 1 cut(s) 160
GsaI CCCAGC 1 cut(s) 97
GsuI CTGGAG 1 cut(s) 153
HaeIII GGCC 1 cut(s) 92
HhaI GCGC 1 cut(s) 161
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HinfI GANTC 2 cut(s) 39, 213
Hpy166II GTNNAC 2 cut(s) 55, 64
Hpy188I TCNGA 1 cut(s) 38
Hpy188III TCNNGA 2 cut(s) 31, 115
Hpy8I GTNNAC 2 cut(s) 55, 64
Hpy99I CGWCG 2 cut(s) 59, 71
HpyAV CCTTC 1 cut(s) 155
HpyF10VI GCNNNNNNNGC 2 cut(s) 108, 238
HspAI GCGC 1 cut(s) 159
Kzo9I GATC 3 cut(s) 33, 70, 132
LmnI GCTCC 1 cut(s) 102
LpnPI CCDG 4 cut(s) 107, 156, 183, 227
LweI GCATC 1 cut(s) 219
MalI GATC 3 cut(s) 35, 72, 134
MboI GATC 3 cut(s) 33, 70, 132
MboII GAAGA 2 cut(s) 35, 127
MflI RGATCY 1 cut(s) 132
MhlI GDGCHC 1 cut(s) 144
MluCI AATT 1 cut(s) 181
MnlI CCTC 5 cut(s) 79, 82, 85, 122, 159
MspR9I CCNGG 1 cut(s) 171
MvaI CCWGG 1 cut(s) 171
MwoI GCNNNNNNNGC 2 cut(s) 108, 238
NdeII GATC 3 cut(s) 33, 70, 132
NlaIV GGNNCC 1 cut(s) 143
PaeR7I CTCGAG 1 cut(s) 83
PcsI WCGNNNNNNNCGW 3 cut(s) 54, 63, 224
PfeI GAWTC 2 cut(s) 39, 213
PflFI GACNNNGTC 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 169
PspFI CCCAGC 1 cut(s) 93
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 1 cut(s) 143
PspPI GGNCC 1 cut(s) 91
PspXI VCTCGAGB 1 cut(s) 83
PsuI RGATCY 1 cut(s) 132
PsyI GACNNNGTC 1 cut(s) 59
SalI GTCGAC 1 cut(s) 53
Sau3AI GATC 3 cut(s) 33, 70, 132
Sau96I GGNCC 1 cut(s) 91
ScrFI CCNGG 1 cut(s) 171
SduI GDGCHC 1 cut(s) 144
SetI ASST 2 cut(s) 99, 147
SfaNI GCATC 1 cut(s) 219
Sfr274I CTCGAG 1 cut(s) 83
SlaI CTCGAG 1 cut(s) 83
SmlI CTYRAG 3 cut(s) 83, 113, 150
SmoI CTYRAG 3 cut(s) 83, 113, 150
Sse9I AATT 1 cut(s) 181
SsiI CCGC 2 cut(s) 100, 109
StyD4I CCNGG 1 cut(s) 169
TaqI TCGA 2 cut(s) 54, 84
TasI AATT 1 cut(s) 181
TfiI GAWTC 2 cut(s) 39, 213
Tth111I GACNNNGTC 1 cut(s) 59
XapI RAATTY 1 cut(s) 181
XhoI CTCGAG 1 cut(s) 83
XmiI GTMKAC 2 cut(s) 54, 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.