Rh2BG678800

gal1,galk

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0005927
Physical Location & Seq
Forward (+)
88894523 .. 88894878
356 bp
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UTR
Exon/CDS
Intron
Rh2BG678800.1

Sequence Viewer

Length: 267 bp
ATGGCTCAAATGATGAGCCACGAAGAACTACCGATCCGAATCCACTTCGCTGTCGACGAAGTCTACAACGATCCCTCGCAACTCGAGGAGGCCCAGGTTTGCTTACACCGCCTCAAGACCAAGTTTCACAAGGTCTTCGGGCACCTTCCTCAAGTCTGCGCTCGCTCTCCAGGGAGAGTGAATTTGATTGGAGAGCATACAGACTATGACGGATTCTCGGTGTTGTCGATGGCGATACGGCAGGATATATACTATTGTGGCGATTAG

Protein Analysis

88

Amino Acids

10.13

Weight (kDa)

5.79

Isoelectric Point (pI)

50.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GalKase_gal_bdg PF10509 41 - 85 1.4e-20 Galactokinase galactose-binding signature
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 141
AccI GTMKAC 2 cut(s) 54, 63
AciI CCGC 1 cut(s) 109
AclWI GGATC 2 cut(s) 28, 65
AcsI RAATTY 1 cut(s) 181
AjnI CCWGG 2 cut(s) 93, 169
AloI GAACNNNNNNTCC 2 cut(s) 18, 50
AlwI GGATC 2 cut(s) 28, 65
Ama87I CYCGRG 1 cut(s) 83
AoxI GGCC 1 cut(s) 90
ApoI RAATTY 1 cut(s) 181
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 91
AvaI CYCGRG 1 cut(s) 83
BaeGI GKGCMC 1 cut(s) 144
BanI GGYRCC 1 cut(s) 141
BbsI GAAGAC 1 cut(s) 127
BccI CCATC 1 cut(s) 223
BceAI ACGGC 1 cut(s) 254
BciT130I CCWGG 2 cut(s) 95, 171
Bme1390I CCNGG 2 cut(s) 95, 171
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 1 cut(s) 143
BmrFI CCNGG 2 cut(s) 95, 171
BpiI GAAGAC 1 cut(s) 127
BpmI CTGGAG 1 cut(s) 153
BpuEI CTTGAG 2 cut(s) 98, 135
BsaBI GATNNNNATC 1 cut(s) 38
BsaJI CCNNGG 2 cut(s) 93, 170
Bse8I GATNNNNATC 1 cut(s) 38
BseBI CCWGG 2 cut(s) 95, 171
BseDI CCNNGG 2 cut(s) 93, 170
BseJI GATNNNNATC 1 cut(s) 38
BseRI GAGGAG 1 cut(s) 101
BseSI GKGCMC 1 cut(s) 144
BshFI GGCC 1 cut(s) 92
BshNI GGYRCC 1 cut(s) 141
BsiHKCI CYCGRG 1 cut(s) 83
BsnI GGCC 1 cut(s) 92
BsoBI CYCGRG 1 cut(s) 83
Bsp1286I GDGCHC 1 cut(s) 144
Bsp143I GATC 2 cut(s) 33, 70
BspACI CCGC 1 cut(s) 109
BspANI GGCC 1 cut(s) 92
BspLI GGNNCC 1 cut(s) 143
BspPI GGATC 2 cut(s) 28, 65
BspT107I GGYRCC 1 cut(s) 141
BssECI CCNNGG 2 cut(s) 93, 170
BssMI GATC 2 cut(s) 33, 70
Bst2UI CCWGG 2 cut(s) 95, 171
BstC8I GCNNGC 1 cut(s) 163
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 2 cut(s) 36, 73
BstMBI GATC 2 cut(s) 33, 70
BstMWI GCNNNNNNNGC 1 cut(s) 108
BstNI CCWGG 2 cut(s) 95, 171
BstSCI CCNGG 2 cut(s) 93, 169
BstSLI GKGCMC 1 cut(s) 144
BstV2I GAAGAC 1 cut(s) 127
BsuRI GGCC 1 cut(s) 92
Cac8I GCNNGC 1 cut(s) 163
CfoI GCGC 1 cut(s) 161
Cfr13I GGNCC 1 cut(s) 91
CviJI RGCY 3 cut(s) 5, 18, 92
CviKI_1 RGCY 3 cut(s) 5, 18, 92
DpnI GATC 2 cut(s) 35, 72
DpnII GATC 2 cut(s) 33, 70
Eco88I CYCGRG 1 cut(s) 83
EcoRII CCWGG 2 cut(s) 93, 169
FaiI YATR 4 cut(s) 198, 207, 248, 250
FblI GTMKAC 2 cut(s) 54, 63
GlaI GCGC 1 cut(s) 160
GsuI CTGGAG 1 cut(s) 153
HaeIII GGCC 1 cut(s) 92
HhaI GCGC 1 cut(s) 161
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HinfI GANTC 2 cut(s) 39, 213
Hpy166II GTNNAC 2 cut(s) 55, 64
Hpy188I TCNGA 1 cut(s) 38
Hpy188III TCNNGA 1 cut(s) 115
Hpy8I GTNNAC 2 cut(s) 55, 64
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 1 cut(s) 155
HpyF10VI GCNNNNNNNGC 1 cut(s) 108
HspAI GCGC 1 cut(s) 159
Kzo9I GATC 2 cut(s) 33, 70
LpnPI CCDG 5 cut(s) 80, 107, 156, 183, 227
MalI GATC 2 cut(s) 35, 72
MboI GATC 2 cut(s) 33, 70
MboII GAAGA 2 cut(s) 35, 127
MhlI GDGCHC 1 cut(s) 144
MluCI AATT 1 cut(s) 181
MnlI CCTC 5 cut(s) 79, 82, 85, 122, 159
MspR9I CCNGG 2 cut(s) 95, 171
MvaI CCWGG 2 cut(s) 95, 171
MwoI GCNNNNNNNGC 1 cut(s) 108
NdeII GATC 2 cut(s) 33, 70
NlaIV GGNNCC 1 cut(s) 143
PaeR7I CTCGAG 1 cut(s) 83
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 224
PfeI GAWTC 2 cut(s) 39, 213
PflFI GACNNNGTC 1 cut(s) 59
Psp6I CCWGG 2 cut(s) 93, 169
PspGI CCWGG 2 cut(s) 93, 169
PspN4I GGNNCC 1 cut(s) 143
PspPI GGNCC 1 cut(s) 91
PspXI VCTCGAGB 1 cut(s) 83
PsyI GACNNNGTC 1 cut(s) 59
SalI GTCGAC 1 cut(s) 53
Sau3AI GATC 2 cut(s) 33, 70
Sau96I GGNCC 1 cut(s) 91
ScrFI CCNGG 2 cut(s) 95, 171
SduI GDGCHC 1 cut(s) 144
SetI ASST 3 cut(s) 99, 135, 147
Sfr274I CTCGAG 1 cut(s) 83
SlaI CTCGAG 1 cut(s) 83
SmlI CTYRAG 3 cut(s) 83, 113, 150
SmoI CTYRAG 3 cut(s) 83, 113, 150
Sse9I AATT 1 cut(s) 181
SsiI CCGC 1 cut(s) 109
StyD4I CCNGG 2 cut(s) 93, 169
TaqI TCGA 3 cut(s) 54, 84, 227
TasI AATT 1 cut(s) 181
TfiI GAWTC 2 cut(s) 39, 213
TspGWI ACGGA 1 cut(s) 225
Tth111I GACNNNGTC 1 cut(s) 59
XapI RAATTY 1 cut(s) 181
XhoI CTCGAG 1 cut(s) 83
XmiI GTMKAC 2 cut(s) 54, 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.