Rh2DG491300

gal1,galk

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0005927
Physical Location & Seq
Forward (+)
71118586 .. 71119324
739 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG491300.1

Sequence Viewer

Length: 349 bp
ATGGCTCAAATGATGAGCCACGAAGAACTCTCGATCCGAATCCACTTCGCTGTCGACGAAGTCTACGACGATCCCTCGCAACTCGAGGAGGCCCAGCTCTGCTTACACGGCCTCAAGACCAAGTTTCACAAGATCTTCGGGCACCTTCCTCAAGTCTGCGCTCGCTCTCCAGGGAGAGTGAATTTGATTGGAGAGCATACAGACTATGACGGATTCTCGGTGTTGCTGATGAACAAGTTCTATTTTTCTTGGATTCTCGTGAAGAAGGAAAACAAAGACACAGTTTCTTTTCTATGGTATTTCCTGTATAACAAATCGGAGAACTGGGGAAGAAAGAAGTATAAAGCTG

Protein Analysis

116

Amino Acids

13.73

Weight (kDa)

7.81

Isoelectric Point (pI)

46.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GalKase_gal_bdg PF10509 41 - 77 8.1e-16 Galactokinase galactose-binding signature
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 141
AccI GTMKAC 2 cut(s) 54, 63
AclWI GGATC 2 cut(s) 28, 65
AcsI RAATTY 1 cut(s) 181
AjnI CCWGG 1 cut(s) 169
AloI GAACNNNNNNTCC 2 cut(s) 18, 50
AluBI AGCT 2 cut(s) 97, 347
AluI AGCT 2 cut(s) 97, 347
AlwI GGATC 2 cut(s) 28, 65
Ama87I CYCGRG 1 cut(s) 83
AoxI GGCC 2 cut(s) 90, 109
ApoI RAATTY 1 cut(s) 181
Asp700I GAANNNNTTC 1 cut(s) 236
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 91
AvaI CYCGRG 1 cut(s) 83
BaeGI GKGCMC 1 cut(s) 144
BanI GGYRCC 1 cut(s) 141
BauI CACGAG 1 cut(s) 257
BceAI ACGGC 1 cut(s) 124
BciT130I CCWGG 1 cut(s) 171
BglII AGATCT 1 cut(s) 132
Bme1390I CCNGG 1 cut(s) 171
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 1 cut(s) 143
BmrFI CCNGG 1 cut(s) 171
BmrI ACTGGG 1 cut(s) 334
BmuI ACTGGG 1 cut(s) 334
BpmI CTGGAG 1 cut(s) 153
BpuEI CTTGAG 2 cut(s) 98, 135
BsaBI GATNNNNATC 1 cut(s) 38
BsaJI CCNNGG 1 cut(s) 170
Bse1I ACTGG 1 cut(s) 329
Bse8I GATNNNNATC 1 cut(s) 38
BseBI CCWGG 1 cut(s) 171
BseDI CCNNGG 1 cut(s) 170
BseJI GATNNNNATC 1 cut(s) 38
BseNI ACTGG 1 cut(s) 329
BseRI GAGGAG 1 cut(s) 101
BseSI GKGCMC 1 cut(s) 144
BseYI CCCAGC 1 cut(s) 93
BshFI GGCC 2 cut(s) 92, 111
BshNI GGYRCC 1 cut(s) 141
BsiHKCI CYCGRG 1 cut(s) 83
BsnI GGCC 2 cut(s) 92, 111
BsoBI CYCGRG 1 cut(s) 83
Bsp1286I GDGCHC 1 cut(s) 144
Bsp143I GATC 3 cut(s) 33, 70, 132
BspANI GGCC 2 cut(s) 92, 111
BspLI GGNNCC 1 cut(s) 143
BspPI GGATC 2 cut(s) 28, 65
BspT107I GGYRCC 1 cut(s) 141
BsrI ACTGG 1 cut(s) 329
BssECI CCNNGG 1 cut(s) 170
BssMI GATC 3 cut(s) 33, 70, 132
BssSI CACGAG 1 cut(s) 257
Bst2BI CACGAG 1 cut(s) 257
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 1 cut(s) 283
BstC8I GCNNGC 1 cut(s) 163
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 3 cut(s) 36, 73, 135
BstMBI GATC 3 cut(s) 33, 70, 132
BstMWI GCNNNNNNNGC 1 cut(s) 108
BstNI CCWGG 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 169
BstSLI GKGCMC 1 cut(s) 144
BstX2I RGATCY 1 cut(s) 132
BstYI RGATCY 1 cut(s) 132
BsuRI GGCC 2 cut(s) 92, 111
Cac8I GCNNGC 1 cut(s) 163
CfoI GCGC 1 cut(s) 161
Cfr13I GGNCC 1 cut(s) 91
CviJI RGCY 6 cut(s) 5, 18, 92, 97, 111, 347
CviKI_1 RGCY 6 cut(s) 5, 18, 92, 97, 111, 347
DpnI GATC 3 cut(s) 35, 72, 134
DpnII GATC 3 cut(s) 33, 70, 132
Eco88I CYCGRG 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 169
FaiI YATR 5 cut(s) 198, 207, 295, 309, 342
FblI GTMKAC 2 cut(s) 54, 63
GlaI GCGC 1 cut(s) 160
GsaI CCCAGC 1 cut(s) 97
GsuI CTGGAG 1 cut(s) 153
HaeIII GGCC 2 cut(s) 92, 111
HhaI GCGC 1 cut(s) 161
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HinfI GANTC 3 cut(s) 39, 213, 253
Hpy166II GTNNAC 2 cut(s) 55, 64
Hpy188I TCNGA 2 cut(s) 38, 319
Hpy188III TCNNGA 3 cut(s) 31, 115, 259
Hpy8I GTNNAC 2 cut(s) 55, 64
Hpy99I CGWCG 2 cut(s) 59, 71
HpyAV CCTTC 2 cut(s) 155, 259
HpyCH4III ACNGT 1 cut(s) 283
HpyF10VI GCNNNNNNNGC 1 cut(s) 108
HspAI GCGC 1 cut(s) 159
Kzo9I GATC 3 cut(s) 33, 70, 132
LpnPI CCDG 5 cut(s) 107, 156, 183, 310, 317
MalI GATC 3 cut(s) 35, 72, 134
MboI GATC 3 cut(s) 33, 70, 132
MboII GAAGA 4 cut(s) 35, 127, 274, 342
MflI RGATCY 1 cut(s) 132
MhlI GDGCHC 1 cut(s) 144
MluCI AATT 1 cut(s) 181
MnlI CCTC 5 cut(s) 79, 82, 85, 122, 159
MroXI GAANNNNTTC 1 cut(s) 236
MspR9I CCNGG 1 cut(s) 171
MvaI CCWGG 1 cut(s) 171
MwoI GCNNNNNNNGC 1 cut(s) 108
NdeII GATC 3 cut(s) 33, 70, 132
NlaIV GGNNCC 1 cut(s) 143
PaeR7I CTCGAG 1 cut(s) 83
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 63
PdmI GAANNNNTTC 1 cut(s) 236
PfeI GAWTC 3 cut(s) 39, 213, 253
PflFI GACNNNGTC 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 169
PspFI CCCAGC 1 cut(s) 93
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 1 cut(s) 143
PspPI GGNCC 1 cut(s) 91
PspXI VCTCGAGB 1 cut(s) 83
PsuI RGATCY 1 cut(s) 132
PsyI GACNNNGTC 1 cut(s) 59
SalI GTCGAC 1 cut(s) 53
Sau3AI GATC 3 cut(s) 33, 70, 132
Sau96I GGNCC 1 cut(s) 91
ScrFI CCNGG 1 cut(s) 171
SduI GDGCHC 1 cut(s) 144
SetI ASST 3 cut(s) 99, 147, 349
Sfr274I CTCGAG 1 cut(s) 83
SlaI CTCGAG 1 cut(s) 83
SmlI CTYRAG 3 cut(s) 83, 113, 150
SmoI CTYRAG 3 cut(s) 83, 113, 150
Sse9I AATT 1 cut(s) 181
StyD4I CCNGG 1 cut(s) 169
TaaI ACNGT 1 cut(s) 283
TaqI TCGA 3 cut(s) 32, 54, 84
TasI AATT 1 cut(s) 181
TfiI GAWTC 3 cut(s) 39, 213, 253
TspDTI ATGAA 1 cut(s) 245
TspGWI ACGGA 1 cut(s) 225
Tth111I GACNNNGTC 1 cut(s) 59
XapI RAATTY 1 cut(s) 181
XhoI CTCGAG 1 cut(s) 83
XmiI GTMKAC 2 cut(s) 54, 63
XmnI GAANNNNTTC 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.