MD10G1029000.v1.1

Abscisic stress-ripening protein 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004221
Physical Location & Seq
Forward (+)
3754277 .. 3756553
2277 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1029000.v1.1.491

Sequence Viewer

Length: 402 bp
ATGTCTGAAGAGAAGCACCACCACGGTCTCTTCCACCACCACAAAGATGAAGACAAACCCTCAGACTACCCTCAGTCTGGCTATAGCGAGGCCACTGTGGCATATGGCGCCACCACCACTCTTGATCCTGAAATTGATTACAAGAAAGAGGAGAAGCACCACAAGCATCTCGAGCACCTCAGCGAGGCCGGTGTTGTTGCTGCCGGCGCTTATGGCTTGCATGAGAAGCACAAGGCAAACAAAGACCCAGAGCATGCCCACAAGCACAAGATAGAAGAAGAGATTGCTGCAGCAGCTGCAGTTGGTGCCGGTGGATTTGCCTTCCATGAGCATCATGAGAAGAAAGAGACAAAGAAAGAAGAGGAAGAGGCTCATGGAAAGAAGCACCACCACCTCTTCTAG

Protein Analysis

134

Amino Acids

15.05

Weight (kDa)

6.17

Isoelectric Point (pI)

52.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABA_WDS PF02496 46 - 123 1.4e-34 ABA/WDS induced protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 107, 305
AclWI GGATC 1 cut(s) 119
AcuI CTGAAG 1 cut(s) 27
AcyI GRCGYC 1 cut(s) 108
AfiI CCNNNNNNNGG 2 cut(s) 77, 184
AluBI AGCT 1 cut(s) 296
AluI AGCT 1 cut(s) 296
Alw21I GWGCWC 1 cut(s) 177
Alw26I GTCTC 2 cut(s) 32, 341
AlwI GGATC 1 cut(s) 119
AlwNI CAGNNNCTG 1 cut(s) 296
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 2 cut(s) 90, 186
ApeKI GCWGC 5 cut(s) 200, 287, 290, 293, 296
AspLEI GCGC 2 cut(s) 110, 209
AvaI CYCGRG 1 cut(s) 170
BanI GGYRCC 2 cut(s) 107, 305
BbsI GAAGAC 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 177
BbvCI CCTCAGC 1 cut(s) 179
BbvI GCAGC 5 cut(s) 187, 274, 283, 302, 305
BcoDI GTCTC 2 cut(s) 32, 341
BfaI CTAG 1 cut(s) 400
BfmI CTRYAG 3 cut(s) 82, 288, 297
BfoI RGCGCY 2 cut(s) 111, 210
BglI GCCNNNNNGGC 1 cut(s) 98
BisI GCNGC 5 cut(s) 201, 288, 291, 294, 297
BlsI GCNGC 5 cut(s) 202, 289, 292, 295, 298
BmeT110I CYCGRG 1 cut(s) 170
BmiI GGNNCC 2 cut(s) 109, 307
BmsI GCATC 2 cut(s) 175, 340
BpiI GAAGAC 1 cut(s) 57
Bpu10I CCTNAGC 1 cut(s) 179
BsaHI GRCGYC 1 cut(s) 108
BsaI GGTCTC 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 22
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 184
Bse118I RCCGGY 3 cut(s) 188, 203, 308
BseDI CCNNGG 1 cut(s) 22
BseLI CCNNNNNNNGG 2 cut(s) 77, 184
BseMII CTCAG 3 cut(s) 75, 86, 193
BseRI GAGGAG 1 cut(s) 164
BseXI GCAGC 5 cut(s) 187, 274, 283, 302, 305
BshFI GGCC 2 cut(s) 92, 188
BshNI GGYRCC 2 cut(s) 107, 305
BsiHKAI GWGCWC 1 cut(s) 177
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 3 cut(s) 189, 204, 309
BslI CCNNNNNNNGG 2 cut(s) 77, 184
BsmAI GTCTC 2 cut(s) 32, 341
BsnI GGCC 2 cut(s) 92, 188
Bso31I GGTCTC 1 cut(s) 32
BsoBI CYCGRG 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 177
Bsp143I GATC 1 cut(s) 124
BspANI GGCC 2 cut(s) 92, 188
BspCNI CTCAG 3 cut(s) 74, 85, 192
BspHI TCATGA 1 cut(s) 334
BspLI GGNNCC 2 cut(s) 109, 307
BspMAI CTGCAG 2 cut(s) 292, 301
BspPI GGATC 1 cut(s) 119
BspT107I GGYRCC 2 cut(s) 107, 305
BspTNI GGTCTC 1 cut(s) 32
BsrFI RCCGGY 3 cut(s) 188, 203, 308
BssAI RCCGGY 3 cut(s) 188, 203, 308
BssECI CCNNGG 1 cut(s) 22
BssMI GATC 1 cut(s) 124
BssNI GRCGYC 1 cut(s) 108
Bst4CI ACNGT 2 cut(s) 26, 97
Bst6I CTCTTC 5 cut(s) 3, 35, 273, 354, 360
BstACI GRCGYC 1 cut(s) 108
BstAPI GCANNNNNTGC 2 cut(s) 296, 305
BstC8I GCNNGC 3 cut(s) 205, 218, 255
BstDEI CTNAG 3 cut(s) 61, 72, 179
BstDSI CCRYGG 1 cut(s) 22
BstENI CCTNNNNNAGG 1 cut(s) 182
BstH2I RGCGCY 2 cut(s) 111, 210
BstHHI GCGC 2 cut(s) 110, 209
BstKTI GATC 1 cut(s) 127
BstMAI GTCTC 2 cut(s) 32, 341
BstMBI GATC 1 cut(s) 124
BstNSI RCATGY 1 cut(s) 257
BstSFI CTRYAG 3 cut(s) 82, 288, 297
BstV1I GCAGC 5 cut(s) 187, 274, 283, 302, 305
BstV2I GAAGAC 1 cut(s) 57
BsuRI GGCC 2 cut(s) 92, 188
BtgI CCRYGG 1 cut(s) 22
BtsIMutI CAGTG 1 cut(s) 93
Cac8I GCNNGC 3 cut(s) 205, 218, 255
CaiI CAGNNNCTG 1 cut(s) 296
CciI TCATGA 1 cut(s) 334
CfoI GCGC 2 cut(s) 110, 209
Cfr10I RCCGGY 3 cut(s) 188, 203, 308
CspCI CAANNNNNGTGG 2 cut(s) 103, 138
CviAII CATG 5 cut(s) 221, 254, 326, 335, 374
CviJI RGCY 6 cut(s) 81, 92, 188, 216, 296, 371
CviKI_1 RGCY 6 cut(s) 81, 92, 188, 216, 296, 371
DdeI CTNAG 3 cut(s) 61, 72, 179
DinI GGCGCC 1 cut(s) 109
DpnI GATC 1 cut(s) 126
DpnII GATC 1 cut(s) 124
Eam1104I CTCTTC 5 cut(s) 3, 35, 273, 354, 360
EarI CTCTTC 5 cut(s) 3, 35, 273, 354, 360
Eco31I GGTCTC 1 cut(s) 32
Eco57I CTGAAG 1 cut(s) 27
Eco88I CYCGRG 1 cut(s) 170
EcoNI CCTNNNNNAGG 1 cut(s) 182
EgeI GGCGCC 1 cut(s) 109
EheI GGCGCC 1 cut(s) 109
FaeI CATG 5 cut(s) 224, 257, 329, 338, 377
FaiI YATR 9 cut(s) 84, 103, 105, 213, 222, 255, 327, 336, 375
FatI CATG 5 cut(s) 220, 253, 325, 334, 373
FauNDI CATATG 1 cut(s) 103
Fnu4HI GCNGC 5 cut(s) 201, 288, 291, 294, 297
Fsp4HI GCNGC 5 cut(s) 201, 288, 291, 294, 297
FspBI CTAG 1 cut(s) 400
GlaI GCGC 2 cut(s) 109, 208
GluI GCNGC 5 cut(s) 201, 288, 291, 294, 297
HaeII RGCGCY 2 cut(s) 111, 210
HaeIII GGCC 2 cut(s) 92, 188
HapII CCGG 3 cut(s) 189, 204, 309
HhaI GCGC 2 cut(s) 110, 209
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 5 cut(s) 224, 257, 329, 338, 377
Hin6I GCGC 2 cut(s) 108, 207
HinP1I GCGC 2 cut(s) 108, 207
HpaII CCGG 3 cut(s) 189, 204, 309
Hpy188I TCNGA 2 cut(s) 7, 64
Hpy188III TCNNGA 4 cut(s) 122, 128, 170, 335
HpyAV CCTTC 1 cut(s) 331
HpyCH4III ACNGT 2 cut(s) 26, 97
HpyCH4V TGCA 3 cut(s) 220, 290, 299
HpyF3I CTNAG 3 cut(s) 61, 72, 179
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 5 cut(s) 224, 257, 329, 338, 377
HspAI GCGC 2 cut(s) 108, 207
KasI GGCGCC 1 cut(s) 107
KroI GCCGGC 1 cut(s) 203
KroNI GCCGGC 1 cut(s) 205
Kzo9I GATC 1 cut(s) 124
LpnPI CCDG 6 cut(s) 63, 141, 202, 217, 261, 322
Lsp1109I GCAGC 5 cut(s) 187, 274, 283, 302, 305
LweI GCATC 2 cut(s) 175, 340
MaeI CTAG 1 cut(s) 400
MalI GATC 1 cut(s) 126
MboI GATC 1 cut(s) 124
MboII GAAGA 9 cut(s) 20, 22, 62, 287, 290, 352, 371, 377, 388
MhlI GDGCHC 1 cut(s) 177
MluCI AATT 1 cut(s) 132
Mly113I GGCGCC 1 cut(s) 108
MnlI CCTC 8 cut(s) 70, 81, 82, 142, 178, 188, 355, 361
MroNI GCCGGC 1 cut(s) 203
MslI CAYNNNNRTG 1 cut(s) 45
MspA1I CMGCKG 1 cut(s) 296
MspI CCGG 3 cut(s) 189, 204, 309
NaeI GCCGGC 1 cut(s) 205
NarI GGCGCC 1 cut(s) 108
NdeI CATATG 1 cut(s) 103
NdeII GATC 1 cut(s) 124
NgoMIV GCCGGC 1 cut(s) 203
NlaIII CATG 5 cut(s) 224, 257, 329, 338, 377
NlaIV GGNNCC 2 cut(s) 109, 307
NspI RCATGY 1 cut(s) 257
PaeI GCATGC 1 cut(s) 257
PaeR7I CTCGAG 1 cut(s) 170
PagI TCATGA 1 cut(s) 334
PdiI GCCGGC 1 cut(s) 205
PkrI GCNGC 5 cut(s) 202, 289, 292, 295, 298
PluTI GGCGCC 1 cut(s) 111
PspN4I GGNNCC 2 cut(s) 109, 307
PstI CTGCAG 2 cut(s) 292, 301
PstNI CAGNNNCTG 1 cut(s) 296
PvuII CAGCTG 1 cut(s) 296
RseI CAYNNNNRTG 1 cut(s) 45
SatI GCNGC 5 cut(s) 201, 288, 291, 294, 297
Sau3AI GATC 1 cut(s) 124
SduI GDGCHC 1 cut(s) 177
SetI ASST 3 cut(s) 180, 298, 396
SfaNI GCATC 2 cut(s) 175, 340
SfcI CTRYAG 3 cut(s) 82, 288, 297
SfoI GGCGCC 1 cut(s) 109
Sfr274I CTCGAG 1 cut(s) 170
SlaI CTCGAG 1 cut(s) 170
SmiMI CAYNNNNRTG 1 cut(s) 45
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
SphI GCATGC 1 cut(s) 257
Sse9I AATT 1 cut(s) 132
SspDI GGCGCC 1 cut(s) 107
SspMI CTAG 1 cut(s) 400
TaaI ACNGT 2 cut(s) 26, 97
TaqI TCGA 1 cut(s) 171
TasI AATT 1 cut(s) 132
TscAI CASTG 1 cut(s) 100
TseI GCWGC 5 cut(s) 200, 287, 290, 293, 296
TspDTI ATGAA 1 cut(s) 63
TspRI CASTG 1 cut(s) 100
XagI CCTNNNNNAGG 1 cut(s) 182
XceI RCATGY 1 cut(s) 257
XhoI CTCGAG 1 cut(s) 170
XspI CTAG 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.