Rroxscaffold_7G00193760

ABA/WDS induced protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0004221
Physical Location & Seq
Forward (+)
35305652 .. 35307127
1476 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00193760.1

Sequence Viewer

Length: 543 bp
ATGTCTGGGGAGAAGCACCACCACGGCCTCTTCCACCACCACAAGGACAACGAGGACAAACCCCTAGAAACCTCCGCCTACGAATCTCAGACCGCTTACGGAGGCGGCGGCTACAGCGAGACCACCGGTTATTCCGCTGAGGAGAGGGTGGCAGGTACAACAGTGAGACCACTGACGCGTATGGCTCAGCTGCTCCCGGCGGTGGCCGATACACCAGTGAGACTACTGGTTACGTCAAGGAGGGACACGGTGGCAAGTACAGCGAGACCACCACCCAAGCTTATGGCACCACTGAGTCCGGAATCGATTACAAGAAGGAGGAGAAGCACCACAAACATCTCGAACAACTCGGCGAGCTCGCATGAGAAGCATAAGGAAAAGAAAGACCCAGAGCATGCCCACAAGCACAAGATAGAAGAGGAGATCGCTGCAGTGGCTGCAGTTGGATCCGGTGGGTTCGCCTTCCACGAGCACCATGAAAAGAAAGAGACCAAGGAAGAAGAGGAAGAGGCTCATGGAAAGAAGCACCACCATCTCTTCTAA

Protein Analysis

180

Amino Acids

20.03

Weight (kDa)

7.03

Isoelectric Point (pI)

63.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABA_WDS PF02496 119 - 170 8.9e-23 ABA/WDS induced protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 143
AccB1I GGYRCC 1 cut(s) 286
AccII CGCG 1 cut(s) 178
AccIII TCCGGA 1 cut(s) 298
AciI CCGC 6 cut(s) 75, 93, 105, 108, 135, 200
AclWI GGATC 2 cut(s) 441, 454
AcoI YGGCCR 1 cut(s) 204
AfaI GTAC 2 cut(s) 157, 259
AfiI CCNNNNNNNGG 2 cut(s) 43, 202
AflIII ACRYGT 1 cut(s) 176
AgeI ACCGGT 1 cut(s) 125
AluBI AGCT 3 cut(s) 190, 280, 357
AluI AGCT 3 cut(s) 190, 280, 357
Alw21I GWGCWC 2 cut(s) 359, 474
Alw26I GTCTC 5 cut(s) 113, 160, 214, 259, 482
AlwI GGATC 2 cut(s) 441, 454
AlwNI CAGNNNCTG 1 cut(s) 437
Aor13HI TCCGGA 1 cut(s) 298
AoxI GGCC 2 cut(s) 25, 204
ApeKI GCWGC 3 cut(s) 190, 428, 437
AsiGI ACCGGT 1 cut(s) 125
AsuC2I CCSGG 1 cut(s) 197
BaeI ACNNNNGTAYC 4 cut(s) 147, 180, 201, 234
BamHI GGATCC 1 cut(s) 446
BanI GGYRCC 1 cut(s) 286
BanII GRGCYC 1 cut(s) 359
BauI CACGAG 1 cut(s) 467
Bbv12I GWGCWC 2 cut(s) 359, 474
BbvCI CCTCAGC 1 cut(s) 138
BbvI GCAGC 3 cut(s) 177, 415, 424
BccI CCATC 1 cut(s) 540
BceAI ACGGC 1 cut(s) 40
BcnI CCSGG 1 cut(s) 197
BcoDI GTCTC 5 cut(s) 113, 160, 214, 259, 482
BfaI CTAG 1 cut(s) 65
BfmI CTRYAG 3 cut(s) 112, 429, 438
BfuAI ACCTGC 1 cut(s) 143
BisI GCNGC 5 cut(s) 106, 109, 191, 429, 438
BlpI GCTNAGC 1 cut(s) 186
BlsI GCNGC 5 cut(s) 107, 110, 192, 430, 439
Bme1390I CCNGG 1 cut(s) 197
BmiI GGNNCC 2 cut(s) 288, 448
BmrFI CCNGG 1 cut(s) 197
Bpu10I CCTNAGC 1 cut(s) 138
Bpu1102I GCTNAGC 1 cut(s) 186
BpuMI CCSGG 1 cut(s) 197
Bsa29I ATCGAT 1 cut(s) 305
BsaI GGTCTC 4 cut(s) 113, 160, 259, 482
BsaJI CCNNGG 2 cut(s) 22, 492
BsaWI WCCGGW 3 cut(s) 125, 298, 449
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 202
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 2 cut(s) 215, 231
BseAI TCCGGA 1 cut(s) 298
BseCI ATCGAT 1 cut(s) 305
BseDI CCNNGG 2 cut(s) 22, 492
BseLI CCNNNNNNNGG 2 cut(s) 43, 202
BseMII CTCAG 4 cut(s) 101, 129, 200, 284
BseNI ACTGG 2 cut(s) 215, 231
BseRI GAGGAG 3 cut(s) 155, 334, 434
BseXI GCAGC 3 cut(s) 177, 415, 424
Bsh1236I CGCG 1 cut(s) 178
BshFI GGCC 2 cut(s) 27, 206
BshNI GGYRCC 1 cut(s) 286
BshTI ACCGGT 1 cut(s) 125
BshVI ATCGAT 1 cut(s) 305
BsiHKAI GWGCWC 2 cut(s) 359, 474
BsiSI CCGG 4 cut(s) 126, 197, 299, 450
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 2 cut(s) 43, 202
BsmAI GTCTC 5 cut(s) 113, 160, 214, 259, 482
BsmFI GGGAC 1 cut(s) 257
BsnI GGCC 2 cut(s) 27, 206
Bso31I GGTCTC 4 cut(s) 113, 160, 259, 482
Bsp1286I GDGCHC 2 cut(s) 359, 474
Bsp13I TCCGGA 1 cut(s) 298
Bsp143I GATC 2 cut(s) 423, 446
Bsp1720I GCTNAGC 1 cut(s) 186
BspACI CCGC 6 cut(s) 75, 93, 105, 108, 135, 200
BspANI GGCC 2 cut(s) 27, 206
BspCNI CTCAG 4 cut(s) 100, 130, 199, 285
BspDI ATCGAT 1 cut(s) 305
BspEI TCCGGA 1 cut(s) 298
BspFNI CGCG 1 cut(s) 178
BspLI GGNNCC 2 cut(s) 288, 448
BspMAI CTGCAG 2 cut(s) 433, 442
BspMI ACCTGC 1 cut(s) 143
BspPI GGATC 2 cut(s) 441, 454
BspT107I GGYRCC 1 cut(s) 286
BspTNI GGTCTC 4 cut(s) 113, 160, 259, 482
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 2 cut(s) 215, 231
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 2 cut(s) 22, 492
BssMI GATC 2 cut(s) 423, 446
BssSI CACGAG 1 cut(s) 467
BssT1I CCWWGG 1 cut(s) 492
Bst2BI CACGAG 1 cut(s) 467
Bst4CI ACNGT 2 cut(s) 163, 250
Bst6I CTCTTC 4 cut(s) 35, 411, 495, 501
BstAPI GCANNNNNTGC 1 cut(s) 437
BstC8I GCNNGC 3 cut(s) 355, 359, 396
BstDEI CTNAG 4 cut(s) 87, 138, 186, 293
BstDSI CCRYGG 1 cut(s) 22
BstFNI CGCG 1 cut(s) 178
BstKTI GATC 2 cut(s) 426, 449
BstMAI GTCTC 5 cut(s) 113, 160, 214, 259, 482
BstMBI GATC 2 cut(s) 423, 446
BstMWI GCNNNNNNNGC 5 cut(s) 114, 260, 367, 434, 437
BstNSI RCATGY 1 cut(s) 398
BstSCI CCNGG 1 cut(s) 195
BstSFI CTRYAG 3 cut(s) 112, 429, 438
BstUI CGCG 1 cut(s) 178
BstV1I GCAGC 3 cut(s) 177, 415, 424
BstX2I RGATCY 1 cut(s) 446
BstXI CCANNNNNNTGG 1 cut(s) 283
BstYI RGATCY 1 cut(s) 446
Bsu15I ATCGAT 1 cut(s) 305
BsuRI GGCC 2 cut(s) 27, 206
BsuTUI ATCGAT 1 cut(s) 305
BtgI CCRYGG 1 cut(s) 22
BtsI GCAGTG 1 cut(s) 438
BtsIMutI CAGTG 5 cut(s) 168, 170, 222, 290, 438
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 3 cut(s) 355, 359, 396
CaiI CAGNNNCTG 1 cut(s) 437
Cfr10I RCCGGY 1 cut(s) 125
ClaI ATCGAT 1 cut(s) 305
CseI GACGC 1 cut(s) 184
Csp6I GTAC 2 cut(s) 156, 258
CspAI ACCGGT 1 cut(s) 125
CviAII CATG 4 cut(s) 362, 395, 476, 515
CviJI RGCY 9 cut(s) 27, 111, 185, 190, 206, 280, 357, 437, 512
CviKI_1 RGCY 9 cut(s) 27, 111, 185, 190, 206, 280, 357, 437, 512
CviQI GTAC 2 cut(s) 156, 258
DdeI CTNAG 4 cut(s) 87, 138, 186, 293
DpnI GATC 2 cut(s) 425, 448
DpnII GATC 2 cut(s) 423, 446
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 4 cut(s) 35, 411, 495, 501
EarI CTCTTC 4 cut(s) 35, 411, 495, 501
EciI GGCGGA 1 cut(s) 64
Ecl136II GAGCTC 1 cut(s) 357
Eco130I CCWWGG 1 cut(s) 492
Eco24I GRGCYC 1 cut(s) 359
Eco31I GGTCTC 4 cut(s) 113, 160, 259, 482
Eco53kI GAGCTC 1 cut(s) 357
EcoICRI GAGCTC 1 cut(s) 357
EcoT14I CCWWGG 1 cut(s) 492
EcoT38I GRGCYC 1 cut(s) 359
ErhI CCWWGG 1 cut(s) 492
FaeI CATG 4 cut(s) 365, 398, 479, 518
FaiI YATR 7 cut(s) 182, 284, 363, 372, 396, 477, 516
FaqI GGGAC 1 cut(s) 257
FatI CATG 4 cut(s) 361, 394, 475, 514
Fnu4HI GCNGC 5 cut(s) 106, 109, 191, 429, 438
FriOI GRGCYC 1 cut(s) 359
Fsp4HI GCNGC 5 cut(s) 106, 109, 191, 429, 438
FspBI CTAG 1 cut(s) 65
GluI GCNGC 5 cut(s) 106, 109, 191, 429, 438
HaeIII GGCC 2 cut(s) 27, 206
HapII CCGG 4 cut(s) 126, 197, 299, 450
HgaI GACGC 1 cut(s) 184
Hin1II CATG 4 cut(s) 365, 398, 479, 518
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 3 cut(s) 83, 295, 302
HpaII CCGG 4 cut(s) 126, 197, 299, 450
Hpy188I TCNGA 1 cut(s) 90
Hpy188III TCNNGA 2 cut(s) 299, 340
HpyAV CCTTC 2 cut(s) 309, 472
HpyCH4III ACNGT 2 cut(s) 163, 250
HpyCH4IV ACGT 1 cut(s) 233
HpyCH4V TGCA 2 cut(s) 431, 440
HpyF10VI GCNNNNNNNGC 5 cut(s) 114, 260, 367, 434, 437
HpyF3I CTNAG 4 cut(s) 87, 138, 186, 293
HpySE526I ACGT 1 cut(s) 233
Hsp92II CATG 4 cut(s) 365, 398, 479, 518
Kpn2I TCCGGA 1 cut(s) 298
Kzo9I GATC 2 cut(s) 423, 446
LmnI GCTCC 1 cut(s) 198
LpnPI CCDG 8 cut(s) 138, 139, 210, 212, 228, 312, 402, 463
Lsp1109I GCAGC 3 cut(s) 177, 415, 424
MaeI CTAG 1 cut(s) 65
MaeII ACGT 1 cut(s) 233
MaeIII GTNAC 1 cut(s) 229
MalI GATC 2 cut(s) 425, 448
MboI GATC 2 cut(s) 423, 446
MboII GAAGA 6 cut(s) 22, 428, 509, 512, 518, 529
MflI RGATCY 1 cut(s) 446
MhlI GDGCHC 2 cut(s) 359, 474
MluI ACGCGT 1 cut(s) 176
MlyI GAGTC 1 cut(s) 304
MmeI TCCRAC 1 cut(s) 424
MroI TCCGGA 1 cut(s) 298
MspA1I CMGCKG 2 cut(s) 137, 190
MspI CCGG 4 cut(s) 126, 197, 299, 450
MspR9I CCNGG 1 cut(s) 197
MvnI CGCG 1 cut(s) 178
MwoI GCNNNNNNNGC 5 cut(s) 114, 260, 367, 434, 437
NciI CCSGG 1 cut(s) 197
NdeII GATC 2 cut(s) 423, 446
NlaIII CATG 4 cut(s) 365, 398, 479, 518
NlaIV GGNNCC 2 cut(s) 288, 448
NmeAIII GCCGAG 1 cut(s) 329
NspI RCATGY 1 cut(s) 398
PaeI GCATGC 1 cut(s) 398
PcsI WCGNNNNNNNCGW 1 cut(s) 465
PfeI GAWTC 2 cut(s) 83, 302
PinAI ACCGGT 1 cut(s) 125
PkrI GCNGC 5 cut(s) 107, 110, 192, 430, 439
PleI GAGTC 1 cut(s) 303
PpsI GAGTC 1 cut(s) 303
Psp124BI GAGCTC 1 cut(s) 359
PspN4I GGNNCC 2 cut(s) 288, 448
PstI CTGCAG 2 cut(s) 433, 442
PstNI CAGNNNCTG 1 cut(s) 437
PsuI RGATCY 1 cut(s) 446
PvuII CAGCTG 1 cut(s) 190
RsaI GTAC 2 cut(s) 157, 259
RsaNI GTAC 2 cut(s) 156, 258
SacI GAGCTC 1 cut(s) 359
SatI GCNGC 5 cut(s) 106, 109, 191, 429, 438
Sau3AI GATC 2 cut(s) 423, 446
SchI GAGTC 1 cut(s) 304
ScrFI CCNGG 1 cut(s) 197
SduI GDGCHC 2 cut(s) 359, 474
SetI ASST 6 cut(s) 74, 157, 192, 236, 282, 359
SfcI CTRYAG 3 cut(s) 112, 429, 438
SphI GCATGC 1 cut(s) 398
SsiI CCGC 6 cut(s) 75, 93, 105, 108, 135, 200
SspMI CTAG 1 cut(s) 65
SstI GAGCTC 1 cut(s) 359
StyD4I CCNGG 1 cut(s) 195
StyI CCWWGG 1 cut(s) 492
TaaI ACNGT 2 cut(s) 163, 250
TaiI ACGT 1 cut(s) 236
TaqI TCGA 2 cut(s) 305, 341
TatI WGTACW 1 cut(s) 257
TauI GCSGC 2 cut(s) 108, 111
TfiI GAWTC 2 cut(s) 83, 302
TscAI CASTG 5 cut(s) 168, 177, 222, 297, 438
TseI GCWGC 3 cut(s) 190, 428, 437
TspDTI ATGAA 1 cut(s) 492
TspGWI ACGGA 1 cut(s) 114
TspRI CASTG 5 cut(s) 168, 177, 222, 297, 438
XceI RCATGY 1 cut(s) 398
XspI CTAG 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.