RchiOBHm_Chr6g0274981

ABA/WDS induced protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004221
Physical Location & Seq
Reverse (-)
36568080 .. 36570232
2153 bp
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UTR
Exon/CDS
Intron
PRQ24670

Sequence Viewer

Length: 573 bp
ATGTCTGAGGAGAAGCACCACCATGGCCTCTTCCACCACCACAAGGACAACGAGGACAGACCCCTAGAAACCTCCGCCTACGAATCTCAGACCGCTTACGGAGGCGGCGGCTACAGCGAGACCACCGGTTATTCCGCTGAGGGAGAGGGTGGCAGGTACAACAGTGAGACCACCGACGCGTATGGCTCAGCTGCTCCCGGCGGTGGCCGATACACCAGCGAGACCACTGGTTACGTCAAGGAGGGACATGGTGGCAAGTACAGCGAGACCACCACCCAAGCTTATGGCACCACTGAGTCCGGAATCGATTACAAGAAGGAGGAGAAGCACCATAAACATCTCGAGCAACTCGGCGAGCTCGGTGCTGCTGCTGCTGGAGCTTATGCTTTGCATGAGAAGCATAAGGAAAAGAAAGACCCAGAGCATGCCCACAAGCACAAGATAGAAGAGGAGATCGCTGCAGTGGCTGCAGTTGGATCCGGTGGGTTCGCCTTCCACGAGCACCATGAAAAGAAAGAGACCAAGGAAGAAGAGGAAGAGGCTCATGGAAAGAAGCACCACCATCTCTTCTAA

Protein Analysis

190

Amino Acids

20.91

Weight (kDa)

5.84

Isoelectric Point (pI)

42.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABA_WDS PF02496 103 - 180 4.7e-36 ABA/WDS induced protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 144
AccB1I GGYRCC 1 cut(s) 287
AccII CGCG 1 cut(s) 179
AccIII TCCGGA 1 cut(s) 299
AciI CCGC 6 cut(s) 75, 93, 105, 108, 135, 201
AclWI GGATC 2 cut(s) 471, 484
AcoI YGGCCR 1 cut(s) 205
AfaI GTAC 2 cut(s) 158, 260
AfiI CCNNNNNNNGG 2 cut(s) 43, 203
AflIII ACRYGT 1 cut(s) 177
AgeI ACCGGT 1 cut(s) 125
AluBI AGCT 4 cut(s) 191, 281, 358, 380
AluI AGCT 4 cut(s) 191, 281, 358, 380
Alw21I GWGCWC 2 cut(s) 360, 504
Alw26I GTCTC 5 cut(s) 113, 161, 215, 260, 512
AlwI GGATC 2 cut(s) 471, 484
AlwNI CAGNNNCTG 1 cut(s) 467
Ama87I CYCGRG 1 cut(s) 341
Aor13HI TCCGGA 1 cut(s) 299
AoxI GGCC 2 cut(s) 25, 205
ApeKI GCWGC 6 cut(s) 191, 365, 368, 371, 458, 467
AsiGI ACCGGT 1 cut(s) 125
AsuC2I CCSGG 1 cut(s) 198
AvaI CYCGRG 1 cut(s) 341
BaeI ACNNNNGTAYC 2 cut(s) 148, 181
BamHI GGATCC 1 cut(s) 476
BanI GGYRCC 1 cut(s) 287
BanII GRGCYC 1 cut(s) 360
BauI CACGAG 1 cut(s) 497
Bbv12I GWGCWC 2 cut(s) 360, 504
BbvCI CCTCAGC 1 cut(s) 138
BbvI GCAGC 6 cut(s) 178, 352, 355, 358, 445, 454
BccI CCATC 1 cut(s) 570
BcgI CGANNNNNNTGC 2 cut(s) 344, 378
BcnI CCSGG 1 cut(s) 198
BcoDI GTCTC 5 cut(s) 113, 161, 215, 260, 512
BfaI CTAG 1 cut(s) 65
BfmI CTRYAG 3 cut(s) 112, 459, 468
BfuAI ACCTGC 1 cut(s) 144
BisI GCNGC 8 cut(s) 106, 109, 192, 366, 369, 372, 459, 468
BlpI GCTNAGC 1 cut(s) 187
BlsI GCNGC 8 cut(s) 107, 110, 193, 367, 370, 373, 460, 469
Bme1390I CCNGG 1 cut(s) 198
BmeT110I CYCGRG 1 cut(s) 341
BmiI GGNNCC 2 cut(s) 289, 478
BmrFI CCNGG 1 cut(s) 198
BpmI CTGGAG 1 cut(s) 396
Bpu10I CCTNAGC 1 cut(s) 138
Bpu1102I GCTNAGC 1 cut(s) 187
BpuMI CCSGG 1 cut(s) 198
Bsa29I ATCGAT 1 cut(s) 306
BsaI GGTCTC 5 cut(s) 113, 161, 215, 260, 512
BsaJI CCNNGG 2 cut(s) 22, 522
BsaWI WCCGGW 3 cut(s) 125, 299, 479
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 203
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 1 cut(s) 232
BseAI TCCGGA 1 cut(s) 299
BseCI ATCGAT 1 cut(s) 306
BseDI CCNNGG 2 cut(s) 22, 522
BseLI CCNNNNNNNGG 2 cut(s) 43, 203
BseMII CTCAG 4 cut(s) 101, 129, 201, 285
BseNI ACTGG 1 cut(s) 232
BseRI GAGGAG 3 cut(s) 23, 335, 464
BseXI GCAGC 6 cut(s) 178, 352, 355, 358, 445, 454
Bsh1236I CGCG 1 cut(s) 179
BshFI GGCC 2 cut(s) 27, 207
BshNI GGYRCC 1 cut(s) 287
BshTI ACCGGT 1 cut(s) 125
BshVI ATCGAT 1 cut(s) 306
BsiHKAI GWGCWC 2 cut(s) 360, 504
BsiHKCI CYCGRG 1 cut(s) 341
BsiSI CCGG 4 cut(s) 126, 198, 300, 480
BslFI GGGAC 1 cut(s) 258
BslI CCNNNNNNNGG 2 cut(s) 43, 203
BsmAI GTCTC 5 cut(s) 113, 161, 215, 260, 512
BsmFI GGGAC 1 cut(s) 258
BsnI GGCC 2 cut(s) 27, 207
Bso31I GGTCTC 5 cut(s) 113, 161, 215, 260, 512
BsoBI CYCGRG 1 cut(s) 341
Bsp1286I GDGCHC 2 cut(s) 360, 504
Bsp13I TCCGGA 1 cut(s) 299
Bsp143I GATC 2 cut(s) 453, 476
Bsp1720I GCTNAGC 1 cut(s) 187
Bsp19I CCATGG 1 cut(s) 22
BspACI CCGC 6 cut(s) 75, 93, 105, 108, 135, 201
BspANI GGCC 2 cut(s) 27, 207
BspCNI CTCAG 4 cut(s) 100, 130, 200, 286
BspDI ATCGAT 1 cut(s) 306
BspEI TCCGGA 1 cut(s) 299
BspFNI CGCG 1 cut(s) 179
BspLI GGNNCC 2 cut(s) 289, 478
BspMAI CTGCAG 2 cut(s) 463, 472
BspMI ACCTGC 1 cut(s) 144
BspPI GGATC 2 cut(s) 471, 484
BspT107I GGYRCC 1 cut(s) 287
BspTNI GGTCTC 5 cut(s) 113, 161, 215, 260, 512
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 1 cut(s) 232
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 2 cut(s) 22, 522
BssMI GATC 2 cut(s) 453, 476
BssSI CACGAG 1 cut(s) 497
BssT1I CCWWGG 2 cut(s) 22, 522
Bst2BI CACGAG 1 cut(s) 497
Bst4CI ACNGT 1 cut(s) 164
Bst6I CTCTTC 4 cut(s) 35, 441, 525, 531
BstAPI GCANNNNNTGC 1 cut(s) 467
BstC8I GCNNGC 2 cut(s) 356, 426
BstDEI CTNAG 5 cut(s) 6, 87, 138, 187, 294
BstDSI CCRYGG 1 cut(s) 22
BstFNI CGCG 1 cut(s) 179
BstKTI GATC 2 cut(s) 456, 479
BstMAI GTCTC 5 cut(s) 113, 161, 215, 260, 512
BstMBI GATC 2 cut(s) 453, 476
BstMWI GCNNNNNNNGC 7 cut(s) 114, 261, 371, 377, 397, 464, 467
BstNSI RCATGY 1 cut(s) 428
BstSCI CCNGG 1 cut(s) 196
BstSFI CTRYAG 3 cut(s) 112, 459, 468
BstUI CGCG 1 cut(s) 179
BstV1I GCAGC 6 cut(s) 178, 352, 355, 358, 445, 454
BstX2I RGATCY 1 cut(s) 476
BstXI CCANNNNNNTGG 1 cut(s) 284
BstYI RGATCY 1 cut(s) 476
Bsu15I ATCGAT 1 cut(s) 306
BsuRI GGCC 2 cut(s) 27, 207
BsuTUI ATCGAT 1 cut(s) 306
BtgI CCRYGG 1 cut(s) 22
BtsI GCAGTG 1 cut(s) 468
BtsIMutI CAGTG 4 cut(s) 169, 225, 291, 468
BveI ACCTGC 1 cut(s) 144
Cac8I GCNNGC 2 cut(s) 356, 426
CaiI CAGNNNCTG 1 cut(s) 467
Cfr10I RCCGGY 1 cut(s) 125
ClaI ATCGAT 1 cut(s) 306
CseI GACGC 1 cut(s) 185
Csp6I GTAC 2 cut(s) 157, 259
CspAI ACCGGT 1 cut(s) 125
CviAII CATG 6 cut(s) 23, 248, 392, 425, 506, 545
CviQI GTAC 2 cut(s) 157, 259
DdeI CTNAG 5 cut(s) 6, 87, 138, 187, 294
DpnI GATC 2 cut(s) 455, 478
DpnII GATC 2 cut(s) 453, 476
EaeI YGGCCR 1 cut(s) 205
Eam1104I CTCTTC 4 cut(s) 35, 441, 525, 531
EarI CTCTTC 4 cut(s) 35, 441, 525, 531
EciI GGCGGA 1 cut(s) 64
Ecl136II GAGCTC 1 cut(s) 358
Eco130I CCWWGG 2 cut(s) 22, 522
Eco24I GRGCYC 1 cut(s) 360
Eco31I GGTCTC 5 cut(s) 113, 161, 215, 260, 512
Eco53kI GAGCTC 1 cut(s) 358
Eco88I CYCGRG 1 cut(s) 341
EcoICRI GAGCTC 1 cut(s) 358
EcoT14I CCWWGG 2 cut(s) 22, 522
EcoT38I GRGCYC 1 cut(s) 360
ErhI CCWWGG 2 cut(s) 22, 522
FaeI CATG 6 cut(s) 26, 251, 395, 428, 509, 548
FaqI GGGAC 1 cut(s) 258
FatI CATG 6 cut(s) 22, 247, 391, 424, 505, 544
Fnu4HI GCNGC 8 cut(s) 106, 109, 192, 366, 369, 372, 459, 468
FriOI GRGCYC 1 cut(s) 360
Fsp4HI GCNGC 8 cut(s) 106, 109, 192, 366, 369, 372, 459, 468
FspBI CTAG 1 cut(s) 65
GluI GCNGC 8 cut(s) 106, 109, 192, 366, 369, 372, 459, 468
GsuI CTGGAG 1 cut(s) 396
HaeIII GGCC 2 cut(s) 27, 207
HapII CCGG 4 cut(s) 126, 198, 300, 480
HgaI GACGC 1 cut(s) 185
Hin1II CATG 6 cut(s) 26, 251, 395, 428, 509, 548
HindIII AAGCTT 1 cut(s) 279
HinfI GANTC 3 cut(s) 83, 296, 303
HpaII CCGG 4 cut(s) 126, 198, 300, 480
Hpy188I TCNGA 2 cut(s) 7, 90
Hpy188III TCNNGA 2 cut(s) 300, 341
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 2 cut(s) 310, 502
HpyCH4III ACNGT 1 cut(s) 164
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 3 cut(s) 391, 461, 470
HpyF10VI GCNNNNNNNGC 7 cut(s) 114, 261, 371, 377, 397, 464, 467
HpyF3I CTNAG 5 cut(s) 6, 87, 138, 187, 294
HpySE526I ACGT 1 cut(s) 234
Hsp92II CATG 6 cut(s) 26, 251, 395, 428, 509, 548
Kpn2I TCCGGA 1 cut(s) 299
Kzo9I GATC 2 cut(s) 453, 476
LmnI GCTCC 2 cut(s) 199, 377
LpnPI CCDG 9 cut(s) 139, 139, 211, 213, 229, 313, 360, 432, 493
Lsp1109I GCAGC 6 cut(s) 178, 352, 355, 358, 445, 454
MaeI CTAG 1 cut(s) 65
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 1 cut(s) 230
MalI GATC 2 cut(s) 455, 478
MboI GATC 2 cut(s) 453, 476
MboII GAAGA 6 cut(s) 22, 458, 539, 542, 548, 559
MflI RGATCY 1 cut(s) 476
MhlI GDGCHC 2 cut(s) 360, 504
MluI ACGCGT 1 cut(s) 177
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 1 cut(s) 454
MroI TCCGGA 1 cut(s) 299
MslI CAYNNNNRTG 1 cut(s) 21
MspA1I CMGCKG 2 cut(s) 137, 191
MspI CCGG 4 cut(s) 126, 198, 300, 480
MspR9I CCNGG 1 cut(s) 198
MvnI CGCG 1 cut(s) 179
MwoI GCNNNNNNNGC 7 cut(s) 114, 261, 371, 377, 397, 464, 467
NciI CCSGG 1 cut(s) 198
NcoI CCATGG 1 cut(s) 22
NdeII GATC 2 cut(s) 453, 476
NlaIII CATG 6 cut(s) 26, 251, 395, 428, 509, 548
NlaIV GGNNCC 2 cut(s) 289, 478
NmeAIII GCCGAG 1 cut(s) 330
NspI RCATGY 1 cut(s) 428
PaeI GCATGC 1 cut(s) 428
PaeR7I CTCGAG 1 cut(s) 341
PcsI WCGNNNNNNNCGW 1 cut(s) 495
PfeI GAWTC 2 cut(s) 83, 303
PinAI ACCGGT 1 cut(s) 125
PkrI GCNGC 8 cut(s) 107, 110, 193, 367, 370, 373, 460, 469
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
Psp124BI GAGCTC 1 cut(s) 360
PspN4I GGNNCC 2 cut(s) 289, 478
PstI CTGCAG 2 cut(s) 463, 472
PstNI CAGNNNCTG 1 cut(s) 467
PsuI RGATCY 1 cut(s) 476
PvuII CAGCTG 1 cut(s) 191
RsaI GTAC 2 cut(s) 158, 260
RsaNI GTAC 2 cut(s) 157, 259
RseI CAYNNNNRTG 1 cut(s) 21
SacI GAGCTC 1 cut(s) 360
SatI GCNGC 8 cut(s) 106, 109, 192, 366, 369, 372, 459, 468
Sau3AI GATC 2 cut(s) 453, 476
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 1 cut(s) 198
SduI GDGCHC 2 cut(s) 360, 504
SetI ASST 7 cut(s) 74, 158, 193, 237, 283, 360, 382
SfcI CTRYAG 3 cut(s) 112, 459, 468
Sfr274I CTCGAG 1 cut(s) 341
SlaI CTCGAG 1 cut(s) 341
SmiMI CAYNNNNRTG 1 cut(s) 21
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
SphI GCATGC 1 cut(s) 428
SsiI CCGC 6 cut(s) 75, 93, 105, 108, 135, 201
SspMI CTAG 1 cut(s) 65
SstI GAGCTC 1 cut(s) 360
StyD4I CCNGG 1 cut(s) 196
StyI CCWWGG 2 cut(s) 22, 522
TaaI ACNGT 1 cut(s) 164
TaiI ACGT 1 cut(s) 237
TaqI TCGA 2 cut(s) 306, 342
TatI WGTACW 1 cut(s) 258
TauI GCSGC 2 cut(s) 108, 111
TfiI GAWTC 2 cut(s) 83, 303
TscAI CASTG 4 cut(s) 169, 232, 298, 468
TseI GCWGC 6 cut(s) 191, 365, 368, 371, 458, 467
TspDTI ATGAA 1 cut(s) 522
TspGWI ACGGA 1 cut(s) 114
TspRI CASTG 4 cut(s) 169, 232, 298, 468
XceI RCATGY 1 cut(s) 428
XhoI CTCGAG 1 cut(s) 341
XspI CTAG 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.