MD10G1065100.v1.1

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001733
Physical Location & Seq
Forward (+)
8907798 .. 8920170
12373 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1065100.v1.1.491

Sequence Viewer

Length: 1668 bp
ATGCAAACCAAAACCCTGATAGCTCACCCTCCTCACTCTCCCCTCCTCCAAAACCCTACTCCCAACGCTCTTTCTCAACCTTCTTTCTCCTTCACCACTCCATCTCTCTCTTTCCCCCCACGCTTTCACCACACACCTGCACTTCGCCTCCCATGGCTACGCTCATCCGCGCCTATACGGCGTCGTAATCGGTTCTGTACCGCCTGCAATGCCCAAACACCTGGAGTCCGCGCGGCAGACGATGGAGGGGACGATGATGCTTCGGTGTCTGCGGAGGGAATTGTGGAGGTTAAGAAAGAAGTGCTGGAGAGTCAGAATCTGTGGAGTCGGATGAAGGAGATTGCAGTGTTTACAGGACCCGCCACTGGGCTTTGGATGTGTGGCCCACTTATGAGTCTCATCGACACCGTCGTCATCGGTCAGGGAAGCTCCATTGAGCTTGCTGCCTTAGGCCCTGGAACTGTGATGTGTGATTATATGAGTTATGTGTTCATGTTCCTTTCCATTGCAACTTCAAACATGGTTGCCACTGCGCTTGCTAGGCGGGATAAAAGTGAAGTGCAACATCAGATATCTATTTTGCTCTTTGTTGGATTGGCCAGTGGCTTCTTAATGGTTTTATTTACAAGATTCTTTGGTTCATGGGCACTAACTGCTTTTACTGGATCAAAGAATGTGGATATTATTCATGCAGCAAATACATATGTTCAGATACGAGGATTGGCATGGCCTGCACTTCTAGTTGGTTGGGTTGCTCAGAGTGCAAGTCTTGGCATGAAAGATTCCTGGGGTCCTTTGAAGGCATTGGCTGTAGCCAGTGCCGTAAATGGAGTTGGTGATGTACTCTTGTGCAGCTTTTTAGGCTATGGTATTGCTGGTGCAGCGTGGGCAACAATGGTGTCACAGGTTATTGCAGGATATATGATGACTGAAGCTCTCAACAAGAAAGGATACAATGCCTATGCCATCTCTGTTCCCTCGTCCAAGGAACTTCTAACAGTACTTGGACTTGCTGCTCCCGTATTTATTACAATGATGTCAAAGGTGGCTTTCTACTCTCTCCTTGTGTATTTTGCGACATCTATGGGAACAAATACCATGGCTGCTCATCAGGTCATGATTCAAACATTCTGCATGTGTACAGTGTGGGGTGAACCTCTCTCTCAAACTGCACAATCGTTTATGCCCGAGTTTATATATGGAGTAAATCGAAGTTTGGCAAAGGCTCGAATGCTGCTCAAGTCACTTGTCATCATTGGAGCTATACTTGGTTCAGTATTAGGGATTGTCGGAACATGTATTCCATGGTTGTTTCCCAATATCTTTACACCTGATCAGATGATCATTCAGGAGATGCATAAAGTGCTGGTACCATATTTTCTTGCATTAATTGTGACGCCCCCCACTCACAGCCTTGAGGGAACATTGCTGGCCGGTCGAGATCTCAGATTTATTAGTCTGTCCATGAGCGGATGCTTTTCCTTAGGTGCACTGCTATTGCTGCTTGTGAGCAGTAGAGGAGTTGGCTTAGCAGGCTGCTGGTGGGCACTGGCAGCATTCCAGTGGAGTCGGTTTTTCCTCGCTCTACAACGGCTGATTTCTCCCAACGGCATACTTTATTCTGAAGATATGACCCGCTTCGAATTGGGAAAACTGAAATCTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

556

Amino Acids

60.09

Weight (kDa)

9.03

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 129 - 277 1.9e-13 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 145
AasI GACNNNNNNGTC 1 cut(s) 410
Acc36I ACCTGC 1 cut(s) 145
Acc65I GGTACC 1 cut(s) 1369
AccB1I GGYRCC 1 cut(s) 1369
AccBSI CCGCTC 1 cut(s) 1470
AccII CGCG 3 cut(s) 170, 231, 233
AciI CCGC 9 cut(s) 168, 201, 229, 233, 272, 360, 544, 1470, 1636
AclWI GGATC 1 cut(s) 673
AcoI YGGCCR 2 cut(s) 597, 1431
AcuI CTGAAG 2 cut(s) 951, 1644
AcyI GRCGYC 2 cut(s) 181, 1397
AfaI GTAC 5 cut(s) 199, 843, 1002, 1141, 1371
AfiI CCNNNNNNNGG 2 cut(s) 365, 366
AflIII ACRYGT 1 cut(s) 1295
AgsI TTSAA 3 cut(s) 516, 799, 1124
AjnI CCWGG 3 cut(s) 220, 454, 785
AloI GAACNNNNNNTCC 2 cut(s) 1285, 1317
AluBI AGCT 6 cut(s) 23, 429, 439, 855, 935, 1262
AluI AGCT 6 cut(s) 23, 429, 439, 855, 935, 1262
Alw21I GWGCWC 1 cut(s) 1492
Alw26I GTCTC 1 cut(s) 401
Alw44I GTGCAC 1 cut(s) 1488
AlwI GGATC 1 cut(s) 673
AlwNI CAGNNNCTG 1 cut(s) 319
Ama87I CYCGRG 1 cut(s) 1187
AoxI GGCC 5 cut(s) 382, 451, 597, 728, 1431
ApaLI GTGCAC 1 cut(s) 1488
AseI ATTAAT 1 cut(s) 1388
Asp718I GGTACC 1 cut(s) 1369
AspLEI GCGC 3 cut(s) 172, 233, 535
AspS9I GGNCC 4 cut(s) 356, 383, 452, 791
AsuHPI GGTGA 5 cut(s) 17, 85, 119, 848, 1163
AsuII TTCGAA 1 cut(s) 1641
AvaI CYCGRG 1 cut(s) 1187
AvaII GGWCC 2 cut(s) 356, 791
AxyI CCTNAGG 2 cut(s) 448, 1483
BaeGI GKGCMC 3 cut(s) 649, 1492, 1549
BalI TGGCCA 1 cut(s) 599
BanI GGYRCC 1 cut(s) 1369
Bbv12I GWGCWC 1 cut(s) 1492
BccI CCATC 3 cut(s) 109, 236, 974
BceAI ACGGC 4 cut(s) 194, 806, 1607, 1624
BciT130I CCWGG 3 cut(s) 222, 456, 787
BciVI GTATCC 1 cut(s) 944
BclI TGATCA 2 cut(s) 1333, 1341
BcoDI GTCTC 1 cut(s) 401
BfaI CTAG 2 cut(s) 540, 740
BfmI CTRYAG 1 cut(s) 810
BfuAI ACCTGC 1 cut(s) 145
BfuI GTATCC 1 cut(s) 944
BglI GCCNNNNNGGC 1 cut(s) 178
BglII AGATCT 1 cut(s) 1441
BlpI GCTNAGC 1 cut(s) 1528
BmcAI AGTACT 1 cut(s) 1002
Bme1390I CCNGG 3 cut(s) 222, 456, 787
Bme18I GGWCC 2 cut(s) 356, 791
BmeT110I CYCGRG 1 cut(s) 1187
BmgT120I GGNCC 4 cut(s) 356, 383, 452, 791
BmiI GGNNCC 3 cut(s) 358, 792, 1371
BmrFI CCNGG 3 cut(s) 222, 456, 787
BmrI ACTGGG 1 cut(s) 375
BmsI GCATC 3 cut(s) 247, 1344, 1463
BmuI ACTGGG 1 cut(s) 375
BpmI CTGGAG 2 cut(s) 243, 326
Bpu1102I GCTNAGC 1 cut(s) 1528
Bpu14I TTCGAA 1 cut(s) 1641
BpuEI CTTGAG 2 cut(s) 1223, 1436
BsaHI GRCGYC 2 cut(s) 181, 1397
BsaJI CCNNGG 6 cut(s) 152, 454, 786, 984, 1098, 1304
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 2 cut(s) 365, 366
Bse118I RCCGGY 1 cut(s) 1433
Bse1I ACTGG 6 cut(s) 370, 600, 667, 816, 1554, 1561
Bse21I CCTNAGG 2 cut(s) 448, 1483
Bse3DI GCAATG 3 cut(s) 214, 504, 1424
BseBI CCWGG 3 cut(s) 222, 456, 787
BseDI CCNNGG 6 cut(s) 152, 454, 786, 984, 1098, 1304
BseGI GGATG 4 cut(s) 164, 336, 381, 1478
BseLI CCNNNNNNNGG 2 cut(s) 365, 366
BseMI GCAATG 3 cut(s) 214, 504, 1424
BseMII CTCAG 2 cut(s) 770, 1459
BseNI ACTGG 6 cut(s) 370, 600, 667, 816, 1554, 1561
BseRI GAGGAG 3 cut(s) 21, 35, 1533
BseSI GKGCMC 3 cut(s) 649, 1492, 1549
BsgI GTGCAG 5 cut(s) 123, 717, 871, 900, 1155
Bsh1236I CGCG 3 cut(s) 170, 231, 233
Bsh1285I CGRYCG 1 cut(s) 1438
BshFI GGCC 5 cut(s) 384, 453, 599, 730, 1433
BshNI GGYRCC 1 cut(s) 1369
BsiEI CGRYCG 1 cut(s) 1438
BsiHKAI GWGCWC 1 cut(s) 1492
BsiHKCI CYCGRG 1 cut(s) 1187
BsiSI CCGG 1 cut(s) 1434
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 2 cut(s) 365, 366
BsmAI GTCTC 1 cut(s) 401
BsmFI GGGAC 1 cut(s) 263
BsmI GAATGC 2 cut(s) 1236, 1556
BsnI GGCC 5 cut(s) 384, 453, 599, 730, 1433
BsoBI CYCGRG 1 cut(s) 1187
Bsp119I TTCGAA 1 cut(s) 1641
Bsp1286I GDGCHC 3 cut(s) 649, 1492, 1549
Bsp1407I TGTACA 1 cut(s) 1139
Bsp143I GATC 4 cut(s) 665, 1333, 1341, 1441
Bsp1720I GCTNAGC 1 cut(s) 1528
Bsp19I CCATGG 3 cut(s) 152, 1098, 1304
BspACI CCGC 9 cut(s) 168, 201, 229, 233, 272, 360, 544, 1470, 1636
BspANI GGCC 5 cut(s) 384, 453, 599, 730, 1433
BspCNI CTCAG 2 cut(s) 769, 1458
BspFNI CGCG 3 cut(s) 170, 231, 233
BspHI TCATGA 1 cut(s) 1116
BspLI GGNNCC 3 cut(s) 358, 792, 1371
BspMI ACCTGC 1 cut(s) 145
BspPI GGATC 1 cut(s) 673
BspT104I TTCGAA 1 cut(s) 1641
BspT107I GGYRCC 1 cut(s) 1369
BsrBI CCGCTC 1 cut(s) 1470
BsrDI GCAATG 3 cut(s) 214, 504, 1424
BsrFI RCCGGY 1 cut(s) 1433
BsrGI TGTACA 1 cut(s) 1139
BsrI ACTGG 6 cut(s) 370, 600, 667, 816, 1554, 1561
BssAI RCCGGY 1 cut(s) 1433
BssECI CCNNGG 6 cut(s) 152, 454, 786, 984, 1098, 1304
BssMI GATC 4 cut(s) 665, 1333, 1341, 1441
BssNI GRCGYC 2 cut(s) 181, 1397
BssT1I CCWWGG 4 cut(s) 152, 984, 1098, 1304
Bst2UI CCWGG 3 cut(s) 222, 456, 787
Bst4CI ACNGT 4 cut(s) 409, 463, 1000, 1144
BstACI GRCGYC 2 cut(s) 181, 1397
BstAPI GCANNNNNTGC 3 cut(s) 653, 731, 1363
BstAUI TGTACA 1 cut(s) 1139
BstBI TTCGAA 1 cut(s) 1641
BstC8I GCNNGC 6 cut(s) 205, 441, 537, 732, 1431, 1534
BstDEI CTNAG 5 cut(s) 448, 756, 1445, 1483, 1528
BstDSI CCRYGG 3 cut(s) 152, 1098, 1304
BstF5I GGATG 4 cut(s) 164, 336, 381, 1478
BstFNI CGCG 3 cut(s) 170, 231, 233
BstHHI GCGC 3 cut(s) 172, 233, 535
BstKTI GATC 4 cut(s) 668, 1336, 1344, 1444
BstMAI GTCTC 1 cut(s) 401
BstMBI GATC 4 cut(s) 665, 1333, 1341, 1441
BstMCI CGRYCG 1 cut(s) 1438
BstNI CCWGG 3 cut(s) 222, 456, 787
BstNSI RCATGY 2 cut(s) 1138, 1299
BstSCI CCNGG 3 cut(s) 220, 454, 785
BstSFI CTRYAG 1 cut(s) 810
BstSLI GKGCMC 3 cut(s) 649, 1492, 1549
BstUI CGCG 3 cut(s) 170, 231, 233
BstX2I RGATCY 1 cut(s) 1441
BstXI CCANNNNNNTGG 1 cut(s) 221
BstYI RGATCY 1 cut(s) 1441
Bsu36I CCTNAGG 2 cut(s) 448, 1483
BsuI GTATCC 1 cut(s) 944
BsuRI GGCC 5 cut(s) 384, 453, 599, 730, 1433
BtgI CCRYGG 3 cut(s) 152, 1098, 1304
BtsCI GGATG 4 cut(s) 164, 336, 381, 1478
BtsI GCAGTG 3 cut(s) 351, 528, 1490
BtsIMutI CAGTG 9 cut(s) 351, 363, 528, 607, 823, 1149, 1490, 1547, 1568
BveI ACCTGC 1 cut(s) 145
Cac8I GCNNGC 6 cut(s) 205, 441, 537, 732, 1431, 1534
CaiI CAGNNNCTG 1 cut(s) 319
CciI TCATGA 1 cut(s) 1116
CfoI GCGC 3 cut(s) 172, 233, 535
Cfr10I RCCGGY 1 cut(s) 1433
Cfr13I GGNCC 4 cut(s) 356, 383, 452, 791
CseI GACGC 2 cut(s) 170, 1405
Csp6I GTAC 5 cut(s) 198, 842, 1001, 1140, 1370
CspCI CAANNNNNGTGG 4 cut(s) 517, 552, 657, 692
CviQI GTAC 5 cut(s) 198, 842, 1001, 1140, 1370
DdeI CTNAG 5 cut(s) 448, 756, 1445, 1483, 1528
DpnI GATC 4 cut(s) 667, 1335, 1343, 1443
DpnII GATC 4 cut(s) 665, 1333, 1341, 1441
DrdI GACNNNNNNGTC 1 cut(s) 410
DseDI GACNNNNNNGTC 1 cut(s) 410
EaeI YGGCCR 2 cut(s) 597, 1431
Eco130I CCWWGG 4 cut(s) 152, 984, 1098, 1304
Eco32I GATATC 1 cut(s) 573
Eco47I GGWCC 2 cut(s) 356, 791
Eco57I CTGAAG 2 cut(s) 951, 1644
Eco81I CCTNAGG 2 cut(s) 448, 1483
Eco88I CYCGRG 1 cut(s) 1187
EcoO109I RGGNCCY 3 cut(s) 356, 452, 791
EcoRII CCWGG 3 cut(s) 220, 454, 785
EcoRV GATATC 1 cut(s) 573
EcoT14I CCWWGG 4 cut(s) 152, 984, 1098, 1304
EcoT22I ATGCAT 1 cut(s) 1359
ErhI CCWWGG 4 cut(s) 152, 984, 1098, 1304
FaqI GGGAC 1 cut(s) 263
FauI CCCGC 3 cut(s) 367, 537, 1643
FauNDI CATATG 1 cut(s) 703
FbaI TGATCA 2 cut(s) 1333, 1341
FokI GGATG 4 cut(s) 151, 343, 388, 1485
FspBI CTAG 2 cut(s) 540, 740
GlaI GCGC 3 cut(s) 171, 232, 534
GsuI CTGGAG 2 cut(s) 243, 326
HaeIII GGCC 5 cut(s) 384, 453, 599, 730, 1433
HapII CCGG 1 cut(s) 1434
HgaI GACGC 2 cut(s) 170, 1405
HhaI GCGC 3 cut(s) 172, 233, 535
Hin1I GRCGYC 2 cut(s) 181, 1397
Hin6I GCGC 3 cut(s) 170, 231, 533
HinP1I GCGC 3 cut(s) 170, 231, 533
HinfI GANTC 9 cut(s) 225, 310, 316, 325, 394, 630, 782, 1120, 1567
HpaII CCGG 1 cut(s) 1434
HphI GGTGA 5 cut(s) 17, 85, 119, 848, 1163
Hpy166II GTNNAC 4 cut(s) 351, 1140, 1154, 1490
Hpy188I TCNGA 9 cut(s) 315, 330, 570, 711, 759, 1292, 1338, 1448, 1624
Hpy188III TCNNGA 3 cut(s) 1117, 1349, 1439
Hpy8I GTNNAC 4 cut(s) 351, 1140, 1154, 1490
Hpy99I CGWCG 2 cut(s) 186, 413
HpyAV CCTTC 4 cut(s) 90, 100, 328, 793
HpyCH4III ACNGT 4 cut(s) 409, 463, 1000, 1144
HpyF3I CTNAG 5 cut(s) 448, 756, 1445, 1483, 1528
Hsp92I GRCGYC 2 cut(s) 181, 1397
HspAI GCGC 3 cut(s) 170, 231, 533
KpnI GGTACC 1 cut(s) 1373
Ksp22I TGATCA 2 cut(s) 1333, 1341
Kzo9I GATC 4 cut(s) 665, 1333, 1341, 1441
LmnI GCTCC 3 cut(s) 434, 1021, 1259
LweI GCATC 3 cut(s) 247, 1344, 1463
MaeI CTAG 2 cut(s) 540, 740
MaeIII GTNAC 3 cut(s) 900, 1242, 1393
MalI GATC 4 cut(s) 667, 1335, 1343, 1443
MbiI CCGCTC 1 cut(s) 1470
MboI GATC 4 cut(s) 665, 1333, 1341, 1441
MboII GAAGA 1 cut(s) 1637
MflI RGATCY 1 cut(s) 1441
MhlI GDGCHC 3 cut(s) 649, 1492, 1549
MlsI TGGCCA 1 cut(s) 599
MluCI AATT 3 cut(s) 279, 1389, 1643
MluNI TGGCCA 1 cut(s) 599
MlyI GAGTC 5 cut(s) 234, 319, 334, 403, 1576
MmeI TCCRAC 3 cut(s) 308, 571, 1270
Mox20I TGGCCA 1 cut(s) 599
Mph1103I ATGCAT 1 cut(s) 1359
MscI TGGCCA 1 cut(s) 599
MseI TTAA 3 cut(s) 291, 611, 1388
MslI CAYNNNNRTG 1 cut(s) 1561
Msp20I TGGCCA 1 cut(s) 599
MspI CCGG 1 cut(s) 1434
MspR9I CCNGG 3 cut(s) 222, 456, 787
Mva1269I GAATGC 2 cut(s) 1236, 1556
MvaI CCWGG 3 cut(s) 222, 456, 787
MvnI CGCG 3 cut(s) 170, 231, 233
NcoI CCATGG 3 cut(s) 152, 1098, 1304
NdeI CATATG 1 cut(s) 703
NdeII GATC 4 cut(s) 665, 1333, 1341, 1441
NlaIV GGNNCC 3 cut(s) 358, 792, 1371
NmuCI GTSAC 3 cut(s) 900, 1242, 1393
NsiI ATGCAT 1 cut(s) 1359
NspI RCATGY 2 cut(s) 1138, 1299
NspV TTCGAA 1 cut(s) 1641
PagI TCATGA 1 cut(s) 1116
PaqCI CACCTGC 1 cut(s) 145
PciI ACATGT 1 cut(s) 1295
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PctI GAATGC 2 cut(s) 1236, 1556
PfeI GAWTC 4 cut(s) 316, 630, 782, 1120
PflFI GACNNNGTC 1 cut(s) 407
PleI GAGTC 5 cut(s) 233, 318, 333, 402, 1575
PpsI GAGTC 5 cut(s) 233, 318, 333, 402, 1575
PpuMI RGGWCCY 2 cut(s) 356, 791
PscI ACATGT 1 cut(s) 1295
PshBI ATTAAT 1 cut(s) 1388
Psp5II RGGWCCY 2 cut(s) 356, 791
Psp6I CCWGG 3 cut(s) 220, 454, 785
PspGI CCWGG 3 cut(s) 220, 454, 785
PspN4I GGNNCC 3 cut(s) 358, 792, 1371
PspPI GGNCC 4 cut(s) 356, 383, 452, 791
PspPPI RGGWCCY 2 cut(s) 356, 791
PstNI CAGNNNCTG 1 cut(s) 319
PsuI RGATCY 1 cut(s) 1441
PsyI GACNNNGTC 1 cut(s) 407
RsaI GTAC 5 cut(s) 199, 843, 1002, 1141, 1371
RsaNI GTAC 5 cut(s) 198, 842, 1001, 1140, 1370
RseI CAYNNNNRTG 1 cut(s) 1561
SaqAI TTAA 3 cut(s) 291, 611, 1388
Sau3AI GATC 4 cut(s) 665, 1333, 1341, 1441
Sau96I GGNCC 4 cut(s) 356, 383, 452, 791
ScaI AGTACT 1 cut(s) 1002
SchI GAGTC 5 cut(s) 234, 319, 334, 403, 1576
ScrFI CCNGG 3 cut(s) 222, 456, 787
SduI GDGCHC 3 cut(s) 649, 1492, 1549
SfaNI GCATC 3 cut(s) 247, 1344, 1463
SfcI CTRYAG 1 cut(s) 810
SfuI TTCGAA 1 cut(s) 1641
SinI GGWCC 2 cut(s) 356, 791
SmiMI CAYNNNNRTG 1 cut(s) 1561
SmlI CTYRAG 2 cut(s) 1238, 1415
SmoI CTYRAG 2 cut(s) 1238, 1415
Sse9I AATT 3 cut(s) 279, 1389, 1643
SsiI CCGC 9 cut(s) 168, 201, 229, 233, 272, 360, 544, 1470, 1636
SspMI CTAG 2 cut(s) 540, 740
StyD4I CCNGG 3 cut(s) 220, 454, 785
StyI CCWWGG 4 cut(s) 152, 984, 1098, 1304
TaaI ACNGT 4 cut(s) 409, 463, 1000, 1144
TaqI TCGA 5 cut(s) 402, 1210, 1228, 1438, 1641
TaqII GACCGA 1 cut(s) 407
TasI AATT 3 cut(s) 279, 1389, 1643
TatI WGTACW 3 cut(s) 841, 1000, 1139
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 4 cut(s) 316, 630, 782, 1120
Tru1I TTAA 3 cut(s) 291, 611, 1388
Tru9I TTAA 3 cut(s) 291, 611, 1388
TscAI CASTG 9 cut(s) 351, 370, 535, 607, 823, 1149, 1497, 1554, 1568
TseFI GTSAC 3 cut(s) 900, 1242, 1393
Tsp45I GTSAC 3 cut(s) 900, 1242, 1393
TspDTI ATGAA 5 cut(s) 347, 481, 630, 677, 791
TspRI CASTG 9 cut(s) 351, 370, 535, 607, 823, 1149, 1497, 1554, 1568
Tth111I GACNNNGTC 1 cut(s) 407
VneI GTGCAC 1 cut(s) 1488
VpaK11BI GGWCC 2 cut(s) 356, 791
VspI ATTAAT 1 cut(s) 1388
XceI RCATGY 2 cut(s) 1138, 1299
XspI CTAG 2 cut(s) 540, 740
ZrmI AGTACT 1 cut(s) 1002
Zsp2I ATGCAT 1 cut(s) 1359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.