Prupe.8G087100_v2.0.a1

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0001733
Physical Location & Seq
Forward (+)
12007120 .. 12013564
6445 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G087100.1

Sequence Viewer

Length: 1656 bp
ATGCAAAGCAAAACCCTGATAGCTCATCACCCTCTTCACTCCCCTCTCCTCCAAAACCTCACTCACACTGCTCTTTCTCACCCCTCACTCTTCTTCACCACCAACCCACCATATTTCTCTTCGACCCCAGGCCTTAACCACACATCTGGCCTCCCAAAGCTACGCTTCTCACCCCCCATACGCCGTCGTAATCGACTGGTGACTGCCTGCATTGTTGACGGGGTTTGTGAAGGAGATGATGGGAAAGATTCTTTAGTGTCTAAAGAAGTTGTGGAGGTGAAGAAAGAAGAGTTGGAGAGTCAGAGCTTATGGAACCAAATGAAGGAGATTATAATGTTTACAGGACCCGCCACTGGGCTTTGGATTTGTGGACCCCTTATGAGCCTCATCGACACTGTTGTGGTTGGCCGGGGAAGCTCTCTTGAGCTTGCTGCTTTAGGCCCTGGAACTGTGATGTGTGATAATATGAGTTATGTGTTCATGTTCCTTTCTATTGCAACTTCAAATATGATTGCTACTGCGCTTGCAAAAGGGGATAGAAATGAAGTGCAGCATCACATATCCATCTTGCTCTTTGTTGGATTGACCTGTGGCTGCTTAATGCTTTTATTTACAAGATTTTTCGGTTCATGGGCGCTAACTGCTTTTGCTGGATCAAAGAATGGGCATATTATTCCTGCAGCAAATACATATGTTCAGATACGAGGTTTGGCATGGCCTGCAATTCTTGTTGGTTGGGTTACTCAGAGTGCAAGTCTTGGCATGAAAGATTCCTGGGGTCCTTTGAAGGCCTTGGCTGTAGCCAGTGTTATAAATGGCATTGGTGATGTAGTCCTTTGCAGTTTTTTAGGCTATGGTATTGCTGGTGCAGCATGGGCAACAATGGTGTCACAGGTTGTTGCAGGATACATGATGATTGAAGCTCTGAACAAGAAAGGATACAATGCCTATGCCATCTCTGTTCCCTCGCCCGAAGAATTTTTAACAGTACTTGGGCTTGCTGCTCCAGTGTTTGTGACAATGATTTCTAAGATAGCTTTCTTCTCTCTCGTTGTATATTTTGCGACGTCAATGGGAACAAACATCACGGCTGCTCATCAGGTCATGATCCAAACATTATTCATCTGTACTGTGTGGGGCGAACCTCTCTCTCAAACTGCACAATCATTTATGCCTGAGTTGATATATGGCGCAAATCGTAGTTTGCCCAAGGCTCGAATGCTGCTCAAGTCACTTGTCATTGTTGGAGCTATCATTGGTTCAGTATTAGGAATTGGAGGAACATGTGTTCCATGGTTGTTTCCGAACATCTTTACACCTGACCAAAAGATCATACAGGAGATGCATAAAGTGCTGATACAATTTTTTTTGGCACTGGCTGTGACACCTGCCATTCTCTGCTTTGAGGGAACACTGCTGGCCGGACGAGATCTAAGATTTATTAGTCTGTCAATGAGTGGATGCTTGTCTTTAGGTGCACTTCTACTGCTGTTTGTGAGTAGTAGAGGATATGGCCTAGCAGGCTGCTGGTGGGCAGTGGTAGGATTTCAATGGGCTCGGCTTTTTCTCTCCCTAGGACGCCTTGTATCTCCTACCGGCATACTTTACTCCGAAGATATGAGCCAGTATAATCTGGAAGAACTCAGAACTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

552

Amino Acids

59.63

Weight (kDa)

8.39

Isoelectric Point (pI)

38.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 332, 812
AarI CACCTGC 1 cut(s) 1396
AatII GACGTC 1 cut(s) 1070
Acc36I ACCTGC 1 cut(s) 1396
AciI CCGC 1 cut(s) 348
AclWI GGATC 2 cut(s) 661, 1102
AcoI YGGCCR 2 cut(s) 406, 1419
AcsI RAATTY 1 cut(s) 977
AcyI GRCGYC 2 cut(s) 1067, 1579
AfaI GTAC 2 cut(s) 990, 1129
AfiI CCNNNNNNNGG 3 cut(s) 322, 353, 354
AflIII ACRYGT 1 cut(s) 1283
AgsI TTSAA 4 cut(s) 504, 787, 920, 1550
AjnI CCWGG 3 cut(s) 127, 442, 773
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
AleI CACNNNNGTG 1 cut(s) 398
AloI GAACNNNNNNTCC 4 cut(s) 1272, 1273, 1304, 1305
AluBI AGCT 8 cut(s) 23, 160, 306, 417, 427, 923, 1037, 1250
AluI AGCT 8 cut(s) 23, 160, 306, 417, 427, 923, 1037, 1250
Alw21I GWGCWC 1 cut(s) 1480
Alw44I GTGCAC 1 cut(s) 1476
AlwI GGATC 2 cut(s) 661, 1102
AoxI GGCC 8 cut(s) 130, 148, 406, 439, 716, 789, 1419, 1513
ApaLI GTGCAC 1 cut(s) 1476
ApeKI GCWGC 9 cut(s) 431, 550, 594, 680, 869, 1001, 1091, 1222, 1524
ApoI RAATTY 1 cut(s) 977
AspA2I CCTAGG 1 cut(s) 1573
AspLEI GCGC 3 cut(s) 523, 637, 1193
AspS9I GGNCC 4 cut(s) 344, 371, 440, 779
AsuC2I CCSGG 1 cut(s) 410
AsuHPI GGTGA 7 cut(s) 20, 71, 88, 162, 211, 289, 836
AvaII GGWCC 3 cut(s) 344, 371, 779
AvrII CCTAGG 1 cut(s) 1573
BaeGI GKGCMC 1 cut(s) 1480
BanII GRGCYC 1 cut(s) 1558
Bbv12I GWGCWC 1 cut(s) 1480
BbvI GCAGC 9 cut(s) 418, 562, 581, 692, 881, 988, 1078, 1209, 1511
BccI CCATC 3 cut(s) 233, 572, 962
BceAI ACGGC 2 cut(s) 168, 1104
BciT130I CCWGG 3 cut(s) 129, 444, 775
BciVI GTATCC 2 cut(s) 899, 932
BcnI CCSGG 1 cut(s) 410
BfaI CTAG 2 cut(s) 1517, 1574
BfmI CTRYAG 2 cut(s) 678, 798
BfoI RGCGCY 1 cut(s) 638
BfuAI ACCTGC 1 cut(s) 1396
BfuI GTATCC 2 cut(s) 899, 932
BglI GCCNNNNNGGC 1 cut(s) 1521
BglII AGATCT 1 cut(s) 1429
BisI GCNGC 9 cut(s) 432, 551, 595, 681, 870, 1002, 1092, 1223, 1525
BlnI CCTAGG 1 cut(s) 1573
BlsI GCNGC 9 cut(s) 433, 552, 596, 682, 871, 1003, 1093, 1224, 1526
BmcAI AGTACT 1 cut(s) 990
Bme1390I CCNGG 4 cut(s) 129, 410, 444, 775
Bme18I GGWCC 3 cut(s) 344, 371, 779
BmgT120I GGNCC 4 cut(s) 344, 371, 440, 779
BmiI GGNNCC 4 cut(s) 314, 346, 373, 780
BmrFI CCNGG 4 cut(s) 129, 410, 444, 775
BmrI ACTGGG 1 cut(s) 363
BmsI GCATC 3 cut(s) 562, 1332, 1451
BmuI ACTGGG 1 cut(s) 363
BpmI CTGGAG 1 cut(s) 990
BpuEI CTTGAG 2 cut(s) 443, 1211
BpuMI CCSGG 1 cut(s) 410
BsaHI GRCGYC 2 cut(s) 1067, 1579
BsaJI CCNNGG 8 cut(s) 127, 409, 442, 774, 792, 1209, 1292, 1573
Bsc4I CCNNNNNNNGG 3 cut(s) 322, 353, 354
Bse118I RCCGGY 1 cut(s) 1595
Bse1I ACTGG 6 cut(s) 201, 358, 804, 1007, 1380, 1624
BseBI CCWGG 3 cut(s) 129, 444, 775
BseDI CCNNGG 8 cut(s) 127, 409, 442, 774, 792, 1209, 1292, 1573
BseGI GGATG 1 cut(s) 1466
BseLI CCNNNNNNNGG 3 cut(s) 322, 353, 354
BseMII CTCAG 2 cut(s) 758, 1167
BseNI ACTGG 6 cut(s) 201, 358, 804, 1007, 1380, 1624
BseRI GAGGAG 1 cut(s) 38
BseSI GKGCMC 1 cut(s) 1480
BseXI GCAGC 9 cut(s) 418, 562, 581, 692, 881, 988, 1078, 1209, 1511
BsgI GTGCAG 3 cut(s) 569, 888, 1143
BshFI GGCC 8 cut(s) 132, 150, 408, 441, 718, 791, 1421, 1515
BsiHKAI GWGCWC 1 cut(s) 1480
BsiSI CCGG 3 cut(s) 409, 1422, 1596
BslI CCNNNNNNNGG 3 cut(s) 322, 353, 354
BsmI GAATGC 1 cut(s) 1224
BsnI GGCC 8 cut(s) 132, 150, 408, 441, 718, 791, 1421, 1515
Bsp1286I GDGCHC 2 cut(s) 1480, 1558
Bsp143I GATC 4 cut(s) 653, 1107, 1329, 1429
Bsp19I CCATGG 1 cut(s) 1292
BspACI CCGC 1 cut(s) 348
BspANI GGCC 8 cut(s) 132, 150, 408, 441, 718, 791, 1421, 1515
BspCNI CTCAG 3 cut(s) 757, 1168, 1656
BspHI TCATGA 1 cut(s) 1104
BspLI GGNNCC 4 cut(s) 314, 346, 373, 780
BspMAI CTGCAG 1 cut(s) 682
BspMI ACCTGC 1 cut(s) 1396
BspPI GGATC 2 cut(s) 661, 1102
BsrFI RCCGGY 1 cut(s) 1595
BsrI ACTGG 6 cut(s) 201, 358, 804, 1007, 1380, 1624
BssAI RCCGGY 1 cut(s) 1595
BssECI CCNNGG 8 cut(s) 127, 409, 442, 774, 792, 1209, 1292, 1573
BssMI GATC 4 cut(s) 653, 1107, 1329, 1429
BssNI GRCGYC 2 cut(s) 1067, 1579
BssT1I CCWWGG 4 cut(s) 792, 1209, 1292, 1573
Bst2UI CCWGG 3 cut(s) 129, 444, 775
Bst4CI ACNGT 5 cut(s) 397, 451, 988, 1132, 1651
Bst6I CTCTTC 4 cut(s) 39, 95, 124, 282
BstACI GRCGYC 2 cut(s) 1067, 1579
BstAPI GCANNNNNTGC 2 cut(s) 719, 1351
BstC8I GCNNGC 7 cut(s) 208, 429, 525, 720, 999, 1419, 1522
BstDEI CTNAG 5 cut(s) 744, 1029, 1176, 1433, 1643
BstDSI CCRYGG 1 cut(s) 1292
BstF5I GGATG 1 cut(s) 1466
BstH2I RGCGCY 1 cut(s) 638
BstHHI GCGC 3 cut(s) 523, 637, 1193
BstKTI GATC 4 cut(s) 656, 1110, 1332, 1432
BstMBI GATC 4 cut(s) 653, 1107, 1329, 1429
BstMWI GCNNNNNNNGC 7 cut(s) 414, 641, 719, 869, 875, 1351, 1521
BstNI CCWGG 3 cut(s) 129, 444, 775
BstNSI RCATGY 1 cut(s) 1287
BstSCI CCNGG 4 cut(s) 127, 408, 442, 773
BstSFI CTRYAG 2 cut(s) 678, 798
BstSLI GKGCMC 1 cut(s) 1480
BstV1I GCAGC 9 cut(s) 418, 562, 581, 692, 881, 988, 1078, 1209, 1511
BstX2I RGATCY 1 cut(s) 1429
BstXI CCANNNNNNTGG 1 cut(s) 146
BstYI RGATCY 1 cut(s) 1429
BsuI GTATCC 2 cut(s) 899, 932
BsuRI GGCC 8 cut(s) 132, 150, 408, 441, 718, 791, 1421, 1515
BtgI CCRYGG 1 cut(s) 1292
BtsCI GGATG 1 cut(s) 1466
BtsI GCAGTG 3 cut(s) 66, 1412, 1542
BtsIMutI CAGTG 8 cut(s) 66, 351, 393, 811, 1014, 1373, 1412, 1542
BveI ACCTGC 1 cut(s) 1396
Cac8I GCNNGC 7 cut(s) 208, 429, 525, 720, 999, 1419, 1522
CciI TCATGA 1 cut(s) 1104
CfoI GCGC 3 cut(s) 523, 637, 1193
Cfr10I RCCGGY 1 cut(s) 1595
Cfr13I GGNCC 4 cut(s) 344, 371, 440, 779
CseI GACGC 1 cut(s) 1587
Csp6I GTAC 2 cut(s) 989, 1128
CviAII CATG 9 cut(s) 481, 630, 714, 763, 873, 910, 1105, 1284, 1293
CviQI GTAC 2 cut(s) 989, 1128
DdeI CTNAG 5 cut(s) 744, 1029, 1176, 1433, 1643
DpnI GATC 4 cut(s) 655, 1109, 1331, 1431
DpnII GATC 4 cut(s) 653, 1107, 1329, 1429
EaeI YGGCCR 2 cut(s) 406, 1419
Eam1104I CTCTTC 4 cut(s) 39, 95, 124, 282
EarI CTCTTC 4 cut(s) 39, 95, 124, 282
Eco130I CCWWGG 4 cut(s) 792, 1209, 1292, 1573
Eco147I AGGCCT 2 cut(s) 132, 791
Eco24I GRGCYC 1 cut(s) 1558
Eco47I GGWCC 3 cut(s) 344, 371, 779
EcoO109I RGGNCCY 3 cut(s) 344, 440, 779
EcoRII CCWGG 3 cut(s) 127, 442, 773
EcoT14I CCWWGG 4 cut(s) 792, 1209, 1292, 1573
EcoT22I ATGCAT 1 cut(s) 1347
EcoT38I GRGCYC 1 cut(s) 1558
ErhI CCWWGG 4 cut(s) 792, 1209, 1292, 1573
FaeI CATG 9 cut(s) 484, 633, 717, 766, 876, 913, 1108, 1287, 1296
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FatI CATG 9 cut(s) 480, 629, 713, 762, 872, 909, 1104, 1283, 1292
FauI CCCGC 1 cut(s) 355
FauNDI CATATG 1 cut(s) 691
Fnu4HI GCNGC 9 cut(s) 432, 551, 595, 681, 870, 1002, 1092, 1223, 1525
FokI GGATG 1 cut(s) 1473
FriOI GRGCYC 1 cut(s) 1558
Fsp4HI GCNGC 9 cut(s) 432, 551, 595, 681, 870, 1002, 1092, 1223, 1525
FspBI CTAG 2 cut(s) 1517, 1574
GlaI GCGC 3 cut(s) 522, 636, 1192
GluI GCNGC 9 cut(s) 432, 551, 595, 681, 870, 1002, 1092, 1223, 1525
GsuI CTGGAG 1 cut(s) 990
HaeII RGCGCY 1 cut(s) 638
HaeIII GGCC 8 cut(s) 132, 150, 408, 441, 718, 791, 1421, 1515
HapII CCGG 3 cut(s) 409, 1422, 1596
HgaI GACGC 1 cut(s) 1587
HhaI GCGC 3 cut(s) 523, 637, 1193
Hin1I GRCGYC 2 cut(s) 1067, 1579
Hin1II CATG 9 cut(s) 484, 633, 717, 766, 876, 913, 1108, 1287, 1296
Hin6I GCGC 3 cut(s) 521, 635, 1191
HinP1I GCGC 3 cut(s) 521, 635, 1191
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 3 cut(s) 248, 298, 770
HpaII CCGG 3 cut(s) 409, 1422, 1596
HphI GGTGA 7 cut(s) 20, 71, 88, 162, 211, 289, 836
Hpy166II GTNNAC 4 cut(s) 217, 339, 371, 1478
Hpy188I TCNGA 7 cut(s) 303, 699, 747, 927, 1305, 1612, 1646
Hpy188III TCNNGA 3 cut(s) 422, 1105, 1634
Hpy8I GTNNAC 4 cut(s) 217, 339, 371, 1478
Hpy99I CGWCG 2 cut(s) 189, 1069
HpyAV CCTTC 3 cut(s) 224, 316, 781
HpyCH4III ACNGT 5 cut(s) 397, 451, 988, 1132, 1651
HpyCH4IV ACGT 1 cut(s) 1067
HpyF10VI GCNNNNNNNGC 7 cut(s) 414, 641, 719, 869, 875, 1351, 1521
HpyF3I CTNAG 5 cut(s) 744, 1029, 1176, 1433, 1643
HpySE526I ACGT 1 cut(s) 1067
Hsp92I GRCGYC 2 cut(s) 1067, 1579
Hsp92II CATG 9 cut(s) 484, 633, 717, 766, 876, 913, 1108, 1287, 1296
HspAI GCGC 3 cut(s) 521, 635, 1191
Kzo9I GATC 4 cut(s) 653, 1107, 1329, 1429
LmnI GCTCC 2 cut(s) 1009, 1247
Lsp1109I GCAGC 9 cut(s) 418, 562, 581, 692, 881, 988, 1078, 1209, 1511
LweI GCATC 3 cut(s) 562, 1332, 1451
MaeI CTAG 2 cut(s) 1517, 1574
MaeII ACGT 1 cut(s) 1067
MaeIII GTNAC 6 cut(s) 199, 739, 888, 1015, 1230, 1381
MalI GATC 4 cut(s) 655, 1109, 1331, 1431
MboI GATC 4 cut(s) 653, 1107, 1329, 1429
MflI RGATCY 1 cut(s) 1429
MhlI GDGCHC 2 cut(s) 1480, 1558
MluCI AATT 4 cut(s) 723, 977, 1272, 1361
MlyI GAGTC 1 cut(s) 307
MmeI TCCRAC 3 cut(s) 273, 559, 1225
Mph1103I ATGCAT 1 cut(s) 1347
MseI TTAA 3 cut(s) 135, 599, 983
MslI CAYNNNNRTG 1 cut(s) 398
MspI CCGG 3 cut(s) 409, 1422, 1596
MspR9I CCNGG 4 cut(s) 129, 410, 444, 775
Mva1269I GAATGC 1 cut(s) 1224
MvaI CCWGG 3 cut(s) 129, 444, 775
MwoI GCNNNNNNNGC 7 cut(s) 414, 641, 719, 869, 875, 1351, 1521
NciI CCSGG 1 cut(s) 410
NcoI CCATGG 1 cut(s) 1292
NdeI CATATG 1 cut(s) 691
NdeII GATC 4 cut(s) 653, 1107, 1329, 1429
NlaIII CATG 9 cut(s) 484, 633, 717, 766, 876, 913, 1108, 1287, 1296
NlaIV GGNNCC 4 cut(s) 314, 346, 373, 780
NmeAIII GCCGAG 1 cut(s) 1537
NmuCI GTSAC 5 cut(s) 199, 888, 1015, 1230, 1381
NsiI ATGCAT 1 cut(s) 1347
NspI RCATGY 1 cut(s) 1287
OliI CACNNNNGTG 1 cut(s) 398
PagI TCATGA 1 cut(s) 1104
PaqCI CACCTGC 1 cut(s) 1396
PceI AGGCCT 2 cut(s) 132, 791
PciI ACATGT 1 cut(s) 1283
PctI GAATGC 1 cut(s) 1224
PfeI GAWTC 2 cut(s) 248, 770
PkrI GCNGC 9 cut(s) 433, 552, 596, 682, 871, 1003, 1093, 1224, 1526
PleI GAGTC 1 cut(s) 306
PpsI GAGTC 1 cut(s) 306
PpuMI RGGWCCY 2 cut(s) 344, 779
PscI ACATGT 1 cut(s) 1283
PsiI TTATAA 2 cut(s) 332, 812
Psp5II RGGWCCY 2 cut(s) 344, 779
Psp6I CCWGG 3 cut(s) 127, 442, 773
PspGI CCWGG 3 cut(s) 127, 442, 773
PspN4I GGNNCC 4 cut(s) 314, 346, 373, 780
PspPI GGNCC 4 cut(s) 344, 371, 440, 779
PspPPI RGGWCCY 2 cut(s) 344, 779
PstI CTGCAG 1 cut(s) 682
PsuI RGATCY 1 cut(s) 1429
RsaI GTAC 2 cut(s) 990, 1129
RsaNI GTAC 2 cut(s) 989, 1128
RseI CAYNNNNRTG 1 cut(s) 398
SaqAI TTAA 3 cut(s) 135, 599, 983
SatI GCNGC 9 cut(s) 432, 551, 595, 681, 870, 1002, 1092, 1223, 1525
Sau3AI GATC 4 cut(s) 653, 1107, 1329, 1429
Sau96I GGNCC 4 cut(s) 344, 371, 440, 779
ScaI AGTACT 1 cut(s) 990
SchI GAGTC 1 cut(s) 307
ScrFI CCNGG 4 cut(s) 129, 410, 444, 775
SduI GDGCHC 2 cut(s) 1480, 1558
SfaNI GCATC 3 cut(s) 562, 1332, 1451
SfcI CTRYAG 2 cut(s) 678, 798
SinI GGWCC 3 cut(s) 344, 371, 779
SmiMI CAYNNNNRTG 1 cut(s) 398
SmlI CTYRAG 2 cut(s) 422, 1226
SmoI CTYRAG 2 cut(s) 422, 1226
Sse9I AATT 4 cut(s) 723, 977, 1272, 1361
SseBI AGGCCT 2 cut(s) 132, 791
SsiI CCGC 1 cut(s) 348
SspMI CTAG 2 cut(s) 1517, 1574
StuI AGGCCT 2 cut(s) 132, 791
StyD4I CCNGG 4 cut(s) 127, 408, 442, 773
StyI CCWWGG 4 cut(s) 792, 1209, 1292, 1573
TaaI ACNGT 5 cut(s) 397, 451, 988, 1132, 1651
TaiI ACGT 1 cut(s) 1070
TaqI TCGA 4 cut(s) 122, 193, 390, 1216
TasI AATT 4 cut(s) 723, 977, 1272, 1361
TatI WGTACW 2 cut(s) 988, 1127
TfiI GAWTC 2 cut(s) 248, 770
Tru1I TTAA 3 cut(s) 135, 599, 983
Tru9I TTAA 3 cut(s) 135, 599, 983
TscAI CASTG 8 cut(s) 73, 358, 400, 811, 1014, 1380, 1419, 1542
TseFI GTSAC 5 cut(s) 199, 888, 1015, 1230, 1381
TseI GCWGC 9 cut(s) 431, 550, 594, 680, 869, 1001, 1091, 1222, 1524
Tsp45I GTSAC 5 cut(s) 199, 888, 1015, 1230, 1381
TspDTI ATGAA 6 cut(s) 335, 469, 558, 618, 779, 1111
TspRI CASTG 8 cut(s) 73, 358, 400, 811, 1014, 1380, 1419, 1542
VneI GTGCAC 1 cut(s) 1476
VpaK11BI GGWCC 3 cut(s) 344, 371, 779
XapI RAATTY 1 cut(s) 977
XceI RCATGY 1 cut(s) 1287
XmaJI CCTAGG 1 cut(s) 1573
XspI CTAG 2 cut(s) 1517, 1574
ZraI GACGTC 1 cut(s) 1068
ZrmI AGTACT 1 cut(s) 990
Zsp2I ATGCAT 1 cut(s) 1347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.