pycom05g04860

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001733
Physical Location & Seq
Forward (+)
5728711 .. 5735279
6569 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g04860.9

Sequence Viewer

Length: 1632 bp
ATGCAAACCAAAACCCTGGTAGCTCACTCTCCTCTTCTCCTCCAAAACCCTACTCGTTTCTTCACAAACCCACCACATTTCTATTTCACCCCACGCTTTAACCACACACCACCTGGCCTCCCCAAACTCCACTTCTCCACCCCATTACGCCGCCGTAACCGGTTCCCAACCGCCTGCATCGGCGACGGGGTTCTTATCGGAGACAATGGGAGCCATGCTTCTCCAGTATCTGAAGAAGTTGAAGTTGTGGAGGTTAAGAGAGAAGAATTGGAGAGTAAGAGCACATGGGATCAGATGAAAGAGATTGCAGTGTTTACAGGACCCGCCACTGGTCTTTGGCTGTGTGGTCCACTTATGAGTCTCATTGACACAGTCGTCATTGGTCAGGGAAGCTCAATTGAGCTTGCTGCTTTGGGACCTGGAACTGTGATGTGTGATAGTCTGAGTTATGTGTTCATGTTCCTTTCTGTTGCAACTTCAAATATGGTTGCTACTGCCCTTGCTAAAGGGGATAGAAATGAAGCTCAACATCACATATCTATCTTGCTCTTTATCGGATTGACATGTGGCTTCGCAATGCTTATATTTACAAGATTCTTCGGTTCATGGGCATTAACTGCTTTCAGTGGACCAAAGAACGCTCATCTTATTCCTGCAGCAAATACATATGTTCAGATACGAGGTTTGGCGTGGCCCGCAGTTCTTATTGGTTTGATTACTCAGAGTGCAAGTCTTGGCATGAAAGATTCATGGGGTCCTTTGAAGGCCTTGGCGGTAGCCAGTGCTATAAATGGCATTGGTGATGTAGTCCTGTGCAGTTTTTTAGGCTATGGTATTGCGGGTGCAGCATGGGCAACAATGGTGTCACAGGTTGTTGCAGGATATATGATGATTCAAGCTTTGAACAATAAAGGATATAATGCATATGCCTTCTCTGTTCCCTCGCCCAAAGAACTTCTAATAGTATTTGAGCTTGCTGCTCCAGTGGTTCTAACAATGATGTCGAAGGTGGCTTTCTACACTCTCATTGTATACTTTGCAACATCAATGGGAACAAACATCATTGCTGCTCATCAGGTCATGATTCAAACATTCTTCATCTGCACGGTGTGGGGTGAACCTCTCTGTCAAACTGCACAATCATTTATGCCTGAGTTGATATATGGAGTAAAGCGTAGTTTGCCTAAGGCTCGGATGCTGCTCAAGTCACTTGTCGTTATTGGAGCTCTACTTGGTTTGGTATTAGGAATTGTGGGAACATCTGTTCCATGGTTTTTTCCTGGTATATTTACACCCGACCAGAAGATCATACAGGAGATGCATAAAGTGCTGATACCATTTTTTTTGGCATTGGCCGTGACACCGGCTATTCTCGCATTTGAAGGAACATTGCTGGCCGGACGAGATCTAAAATTTATTAGTCTGTCAATGAGTGGATGTTTTAGTTTAGGTGCACTTCTACTGCTGTTTGTTAGTAGTAGAGGATGTGGTTTAGCAGGCTGTTGGTGGGCACTGGTAGGATTTCAATGGGCTCGGCTTTTTCTGTCTTTACAGCGCGCTACATCCACCAACGGCATACTTTACTCTGAAGATACGAGCCGGTATCAACAAGAAAAACTCAGAGCTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

544

Amino Acids

58.92

Weight (kDa)

8.93

Isoelectric Point (pI)

34.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 374
AccI GTMKAC 1 cut(s) 1032
AccII CGCG 1 cut(s) 1557
AciI CCGC 6 cut(s) 151, 171, 324, 696, 773, 839
AclWI GGATC 1 cut(s) 297
AcoI YGGCCR 2 cut(s) 1353, 1395
AcsI RAATTY 1 cut(s) 1412
AcuI CTGAAG 2 cut(s) 252, 1608
AdeI CACNNNGTG 1 cut(s) 1110
AfiI CCNNNNNNNGG 1 cut(s) 329
AflIII ACRYGT 1 cut(s) 563
AgeI ACCGGT 1 cut(s) 159
AgsI TTSAA 8 cut(s) 242, 480, 763, 896, 904, 1088, 1382, 1526
AjnI CCWGG 4 cut(s) 15, 112, 418, 1279
AjuI GAANNNNNNNTTGG 2 cut(s) 116, 148
AloI GAACNNNNNNTCC 4 cut(s) 1248, 1249, 1280, 1281
AluBI AGCT 8 cut(s) 23, 393, 403, 524, 899, 973, 1226, 1625
AluI AGCT 8 cut(s) 23, 393, 403, 524, 899, 973, 1226, 1625
Alw21I GWGCWC 3 cut(s) 284, 1228, 1456
Alw26I GTCTC 2 cut(s) 195, 365
Alw44I GTGCAC 1 cut(s) 1452
AlwI GGATC 1 cut(s) 297
AlwNI CAGNNNCTG 1 cut(s) 230
AoxI GGCC 5 cut(s) 115, 692, 765, 1353, 1395
ApaLI GTGCAC 1 cut(s) 1452
ApeKI GCWGC 6 cut(s) 407, 656, 845, 977, 1067, 1198
ApoI RAATTY 1 cut(s) 1412
AsiGI ACCGGT 1 cut(s) 159
AspLEI GCGC 2 cut(s) 1557, 1559
AspS9I GGNCC 6 cut(s) 320, 347, 416, 629, 693, 755
AsuHPI GGTGA 3 cut(s) 79, 812, 1127
AvaII GGWCC 5 cut(s) 320, 347, 416, 629, 755
AxyI CCTNAGG 1 cut(s) 1185
BaeGI GKGCMC 2 cut(s) 1456, 1513
BanII GRGCYC 2 cut(s) 1228, 1534
Bbv12I GWGCWC 3 cut(s) 284, 1228, 1456
BbvI GCAGC 6 cut(s) 394, 668, 857, 964, 1054, 1185
BceAI ACGGC 3 cut(s) 138, 1340, 1588
BciT130I CCWGG 4 cut(s) 17, 114, 420, 1281
BcoDI GTCTC 2 cut(s) 195, 365
BfmI CTRYAG 1 cut(s) 654
BglII AGATCT 1 cut(s) 1405
BisI GCNGC 7 cut(s) 151, 408, 657, 846, 978, 1068, 1199
BlsI GCNGC 7 cut(s) 152, 409, 658, 847, 979, 1069, 1200
Bme1390I CCNGG 4 cut(s) 17, 114, 420, 1281
Bme18I GGWCC 5 cut(s) 320, 347, 416, 629, 755
BmgT120I GGNCC 6 cut(s) 320, 347, 416, 629, 693, 755
BmiI GGNNCC 5 cut(s) 164, 212, 322, 417, 756
BmrFI CCNGG 4 cut(s) 17, 114, 420, 1281
BmsI GCATC 3 cut(s) 186, 1185, 1308
BpmI CTGGAG 2 cut(s) 207, 966
BpuEI CTTGAG 1 cut(s) 1187
BsaJI CCNNGG 3 cut(s) 15, 768, 1268
BsaWI WCCGGW 1 cut(s) 159
BsaXI ACNNNNNCTCC 2 cut(s) 102, 132
Bsc4I CCNNNNNNNGG 1 cut(s) 329
Bse118I RCCGGY 3 cut(s) 159, 1363, 1599
Bse1I ACTGG 5 cut(s) 224, 334, 780, 983, 1518
Bse21I CCTNAGG 1 cut(s) 1185
Bse3DI GCAATG 3 cut(s) 582, 1062, 1388
BseBI CCWGG 4 cut(s) 17, 114, 420, 1281
BseDI CCNNGG 3 cut(s) 15, 768, 1268
BseGI GGATG 4 cut(s) 1200, 1442, 1490, 1562
BseLI CCNNNNNNNGG 1 cut(s) 329
BseMI GCAATG 3 cut(s) 582, 1062, 1388
BseMII CTCAG 3 cut(s) 434, 734, 1143
BseNI ACTGG 5 cut(s) 224, 334, 780, 983, 1518
BsePI GCGCGC 1 cut(s) 1555
BseRI GAGGAG 2 cut(s) 21, 29
BseSI GKGCMC 2 cut(s) 1456, 1513
BseXI GCAGC 6 cut(s) 394, 668, 857, 964, 1054, 1185
BsgI GTGCAG 4 cut(s) 835, 864, 1087, 1119
Bsh1236I CGCG 1 cut(s) 1557
BshFI GGCC 5 cut(s) 117, 694, 767, 1355, 1397
BshTI ACCGGT 1 cut(s) 159
BsiHKAI GWGCWC 3 cut(s) 284, 1228, 1456
BsiSI CCGG 4 cut(s) 160, 1364, 1398, 1600
BslFI GGGAC 1 cut(s) 429
BslI CCNNNNNNNGG 1 cut(s) 329
BsmAI GTCTC 2 cut(s) 195, 365
BsmFI GGGAC 1 cut(s) 429
BsnI GGCC 5 cut(s) 117, 694, 767, 1355, 1397
Bsp1286I GDGCHC 5 cut(s) 284, 1228, 1456, 1513, 1534
Bsp143I GATC 3 cut(s) 289, 1305, 1405
Bsp19I CCATGG 1 cut(s) 1268
BspACI CCGC 6 cut(s) 151, 171, 324, 696, 773, 839
BspANI GGCC 5 cut(s) 117, 694, 767, 1355, 1397
BspCNI CTCAG 4 cut(s) 435, 733, 1144, 1632
BspFNI CGCG 1 cut(s) 1557
BspHI TCATGA 1 cut(s) 1080
BspLI GGNNCC 5 cut(s) 164, 212, 322, 417, 756
BspMAI CTGCAG 1 cut(s) 658
BspPI GGATC 1 cut(s) 297
BsrDI GCAATG 3 cut(s) 582, 1062, 1388
BsrFI RCCGGY 3 cut(s) 159, 1363, 1599
BsrI ACTGG 5 cut(s) 224, 334, 780, 983, 1518
BssAI RCCGGY 3 cut(s) 159, 1363, 1599
BssECI CCNNGG 3 cut(s) 15, 768, 1268
BssHII GCGCGC 1 cut(s) 1555
BssMI GATC 3 cut(s) 289, 1305, 1405
BssNAI GTATAC 1 cut(s) 1033
BssT1I CCWWGG 2 cut(s) 768, 1268
Bst1107I GTATAC 1 cut(s) 1033
Bst2UI CCWGG 4 cut(s) 17, 114, 420, 1281
Bst4CI ACNGT 3 cut(s) 373, 427, 1108
Bst6I CTCTTC 1 cut(s) 39
BstAPI GCANNNNNTGC 2 cut(s) 617, 1327
BstC8I GCNNGC 7 cut(s) 175, 405, 696, 975, 1395, 1498, 1557
BstDEI CTNAG 5 cut(s) 443, 720, 1152, 1185, 1619
BstDSI CCRYGG 1 cut(s) 1268
BstF5I GGATG 4 cut(s) 1200, 1442, 1490, 1562
BstFNI CGCG 1 cut(s) 1557
BstHHI GCGC 2 cut(s) 1557, 1559
BstKTI GATC 3 cut(s) 292, 1308, 1408
BstMAI GTCTC 2 cut(s) 195, 365
BstMBI GATC 3 cut(s) 289, 1305, 1405
BstMWI GCNNNNNNNGC 7 cut(s) 617, 695, 845, 851, 1180, 1327, 1373
BstNI CCWGG 4 cut(s) 17, 114, 420, 1281
BstNSI RCATGY 1 cut(s) 567
BstSCI CCNGG 4 cut(s) 15, 112, 418, 1279
BstSFI CTRYAG 1 cut(s) 654
BstSLI GKGCMC 2 cut(s) 1456, 1513
BstUI CGCG 1 cut(s) 1557
BstV1I GCAGC 6 cut(s) 394, 668, 857, 964, 1054, 1185
BstX2I RGATCY 1 cut(s) 1405
BstXI CCANNNNNNTGG 1 cut(s) 16
BstYI RGATCY 1 cut(s) 1405
BstZ17I GTATAC 1 cut(s) 1033
Bsu36I CCTNAGG 1 cut(s) 1185
BsuRI GGCC 5 cut(s) 117, 694, 767, 1355, 1397
BtgI CCRYGG 1 cut(s) 1268
BtsCI GGATG 4 cut(s) 1200, 1442, 1490, 1562
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 6 cut(s) 315, 327, 631, 787, 990, 1511
Cac8I GCNNGC 7 cut(s) 175, 405, 696, 975, 1395, 1498, 1557
CaiI CAGNNNCTG 1 cut(s) 230
CciI TCATGA 1 cut(s) 1080
CfoI GCGC 2 cut(s) 1557, 1559
Cfr10I RCCGGY 3 cut(s) 159, 1363, 1599
Cfr13I GGNCC 6 cut(s) 320, 347, 416, 629, 693, 755
CspAI ACCGGT 1 cut(s) 159
DdeI CTNAG 5 cut(s) 443, 720, 1152, 1185, 1619
DpnI GATC 3 cut(s) 291, 1307, 1407
DpnII GATC 3 cut(s) 289, 1305, 1405
DraIII CACNNNGTG 1 cut(s) 1110
DrdI GACNNNNNNGTC 1 cut(s) 374
DseDI GACNNNNNNGTC 1 cut(s) 374
EaeI YGGCCR 2 cut(s) 1353, 1395
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
Ecl136II GAGCTC 1 cut(s) 1226
Eco130I CCWWGG 2 cut(s) 768, 1268
Eco147I AGGCCT 1 cut(s) 767
Eco24I GRGCYC 2 cut(s) 1228, 1534
Eco47I GGWCC 5 cut(s) 320, 347, 416, 629, 755
Eco53kI GAGCTC 1 cut(s) 1226
Eco57I CTGAAG 2 cut(s) 252, 1608
Eco81I CCTNAGG 1 cut(s) 1185
EcoICRI GAGCTC 1 cut(s) 1226
EcoO109I RGGNCCY 3 cut(s) 320, 416, 755
EcoRII CCWGG 4 cut(s) 15, 112, 418, 1279
EcoT14I CCWWGG 2 cut(s) 768, 1268
EcoT22I ATGCAT 2 cut(s) 925, 1323
EcoT38I GRGCYC 2 cut(s) 1228, 1534
ErhI CCWWGG 2 cut(s) 768, 1268
FaqI GGGAC 1 cut(s) 429
FauI CCCGC 3 cut(s) 331, 703, 832
FauNDI CATATG 2 cut(s) 667, 925
FblI GTMKAC 1 cut(s) 1032
Fnu4HI GCNGC 7 cut(s) 151, 408, 657, 846, 978, 1068, 1199
FokI GGATG 4 cut(s) 1207, 1449, 1497, 1549
FriOI GRGCYC 2 cut(s) 1228, 1534
Fsp4HI GCNGC 7 cut(s) 151, 408, 657, 846, 978, 1068, 1199
GlaI GCGC 2 cut(s) 1556, 1558
GluI GCNGC 7 cut(s) 151, 408, 657, 846, 978, 1068, 1199
GsuI CTGGAG 2 cut(s) 207, 966
HaeIII GGCC 5 cut(s) 117, 694, 767, 1355, 1397
HapII CCGG 4 cut(s) 160, 1364, 1398, 1600
HhaI GCGC 2 cut(s) 1557, 1559
Hin6I GCGC 2 cut(s) 1555, 1557
HinP1I GCGC 2 cut(s) 1555, 1557
HindIII AAGCTT 1 cut(s) 897
HinfI GANTC 5 cut(s) 358, 594, 746, 892, 1084
HpaII CCGG 4 cut(s) 160, 1364, 1398, 1600
HphI GGTGA 3 cut(s) 79, 812, 1127
Hpy166II GTNNAC 6 cut(s) 315, 350, 629, 1033, 1118, 1454
Hpy188III TCNNGA 1 cut(s) 1081
Hpy8I GTNNAC 6 cut(s) 315, 350, 629, 1033, 1118, 1454
Hpy99I CGWCG 1 cut(s) 188
HpyAV CCTTC 4 cut(s) 757, 940, 1000, 1376
HpyCH4III ACNGT 3 cut(s) 373, 427, 1108
HpyF10VI GCNNNNNNNGC 7 cut(s) 617, 695, 845, 851, 1180, 1327, 1373
HpyF3I CTNAG 5 cut(s) 443, 720, 1152, 1185, 1619
HspAI GCGC 2 cut(s) 1555, 1557
Kzo9I GATC 3 cut(s) 289, 1305, 1405
LmnI GCTCC 3 cut(s) 210, 985, 1223
Lsp1109I GCAGC 6 cut(s) 394, 668, 857, 964, 1054, 1185
LweI GCATC 3 cut(s) 186, 1185, 1308
MaeIII GTNAC 4 cut(s) 155, 864, 1206, 1357
MalI GATC 3 cut(s) 291, 1307, 1407
MboI GATC 3 cut(s) 289, 1305, 1405
MboII GAAGA 8 cut(s) 26, 52, 245, 275, 589, 1087, 1315, 1601
MfeI CAATTG 1 cut(s) 396
MflI RGATCY 1 cut(s) 1405
MhlI GDGCHC 5 cut(s) 284, 1228, 1456, 1513, 1534
MluCI AATT 4 cut(s) 266, 396, 1248, 1412
MlyI GAGTC 1 cut(s) 367
MnlI CCTC 8 cut(s) 42, 50, 128, 244, 674, 952, 1131, 1475
Mph1103I ATGCAT 2 cut(s) 925, 1323
MseI TTAA 3 cut(s) 99, 255, 614
MspI CCGG 4 cut(s) 160, 1364, 1398, 1600
MspR9I CCNGG 4 cut(s) 17, 114, 420, 1281
MunI CAATTG 1 cut(s) 396
MvaI CCWGG 4 cut(s) 17, 114, 420, 1281
MvnI CGCG 1 cut(s) 1557
MwoI GCNNNNNNNGC 7 cut(s) 617, 695, 845, 851, 1180, 1327, 1373
NcoI CCATGG 1 cut(s) 1268
NdeI CATATG 2 cut(s) 667, 925
NdeII GATC 3 cut(s) 289, 1305, 1405
NlaIV GGNNCC 5 cut(s) 164, 212, 322, 417, 756
NmeAIII GCCGAG 1 cut(s) 1513
NmuCI GTSAC 3 cut(s) 864, 1206, 1357
NsiI ATGCAT 2 cut(s) 925, 1323
NspI RCATGY 1 cut(s) 567
PagI TCATGA 1 cut(s) 1080
PauI GCGCGC 1 cut(s) 1555
PceI AGGCCT 1 cut(s) 767
PciI ACATGT 1 cut(s) 563
PfeI GAWTC 4 cut(s) 594, 746, 892, 1084
PflFI GACNNNGTC 1 cut(s) 371
PinAI ACCGGT 1 cut(s) 159
PkrI GCNGC 7 cut(s) 152, 409, 658, 847, 979, 1069, 1200
PleI GAGTC 1 cut(s) 366
PpsI GAGTC 1 cut(s) 366
PpuMI RGGWCCY 3 cut(s) 320, 416, 755
PscI ACATGT 1 cut(s) 563
Psp124BI GAGCTC 1 cut(s) 1228
Psp5II RGGWCCY 3 cut(s) 320, 416, 755
Psp6I CCWGG 4 cut(s) 15, 112, 418, 1279
PspGI CCWGG 4 cut(s) 15, 112, 418, 1279
PspN4I GGNNCC 5 cut(s) 164, 212, 322, 417, 756
PspPI GGNCC 6 cut(s) 320, 347, 416, 629, 693, 755
PspPPI RGGWCCY 3 cut(s) 320, 416, 755
PstI CTGCAG 1 cut(s) 658
PstNI CAGNNNCTG 1 cut(s) 230
PsuI RGATCY 1 cut(s) 1405
PsyI GACNNNGTC 1 cut(s) 371
PteI GCGCGC 1 cut(s) 1555
SacI GAGCTC 1 cut(s) 1228
SaqAI TTAA 3 cut(s) 99, 255, 614
SatI GCNGC 7 cut(s) 151, 408, 657, 846, 978, 1068, 1199
Sau3AI GATC 3 cut(s) 289, 1305, 1405
Sau96I GGNCC 6 cut(s) 320, 347, 416, 629, 693, 755
SchI GAGTC 1 cut(s) 367
ScrFI CCNGG 4 cut(s) 17, 114, 420, 1281
SduI GDGCHC 5 cut(s) 284, 1228, 1456, 1513, 1534
SfaNI GCATC 3 cut(s) 186, 1185, 1308
SfcI CTRYAG 1 cut(s) 654
SinI GGWCC 5 cut(s) 320, 347, 416, 629, 755
SmlI CTYRAG 1 cut(s) 1202
SmoI CTYRAG 1 cut(s) 1202
Sse9I AATT 4 cut(s) 266, 396, 1248, 1412
SseBI AGGCCT 1 cut(s) 767
SsiI CCGC 6 cut(s) 151, 171, 324, 696, 773, 839
SstI GAGCTC 1 cut(s) 1228
StuI AGGCCT 1 cut(s) 767
StyD4I CCNGG 4 cut(s) 15, 112, 418, 1279
StyI CCWWGG 2 cut(s) 768, 1268
TaaI ACNGT 3 cut(s) 373, 427, 1108
TaqI TCGA 1 cut(s) 1004
TasI AATT 4 cut(s) 266, 396, 1248, 1412
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 4 cut(s) 594, 746, 892, 1084
Tru1I TTAA 3 cut(s) 99, 255, 614
Tru9I TTAA 3 cut(s) 99, 255, 614
TscAI CASTG 6 cut(s) 315, 334, 631, 787, 990, 1518
TseFI GTSAC 3 cut(s) 864, 1206, 1357
TseI GCWGC 6 cut(s) 407, 656, 845, 977, 1067, 1198
Tsp45I GTSAC 3 cut(s) 864, 1206, 1357
TspDTI ATGAA 7 cut(s) 311, 445, 534, 594, 738, 755, 1087
TspRI CASTG 6 cut(s) 315, 334, 631, 787, 990, 1518
Tth111I GACNNNGTC 1 cut(s) 371
VneI GTGCAC 1 cut(s) 1452
VpaK11BI GGWCC 5 cut(s) 320, 347, 416, 629, 755
XapI RAATTY 1 cut(s) 1412
XceI RCATGY 1 cut(s) 567
XcmI CCANNNNNNNNNTGG 1 cut(s) 110
XmiI GTMKAC 1 cut(s) 1032
Zsp2I ATGCAT 2 cut(s) 925, 1323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.