Rmu_sc0002938.1_g000046

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002938.1
OG0001733
Physical Location & Seq
Forward (+)
161573 .. 167427
5855 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002938.1_g000046.1.cds

Sequence Viewer

Length: 1623 bp
atgcaagctagaaccctccacctcatttctcactctctctcttctcaaccttgttgtctcttctccactactcctccaccttcttcctctctcttcttcaaccacacacctcgatttccgagctcacgctcacgctgcttctcctcctcatcctcagctccaatacgacgccgttcgattcgccctgtaatcgcctgcattggcgatggcggggacatttcggggtctggagaagctgcggaggttaaaggaggagagttggagagtcagagtatatggaatcagatgaaggagattgtcatgtttactgggccggctaccgggatctggatcagcggcccacttatgagtctcatcgacaccgtcgtcgtcggtcagggaagctccattgagcttgccgcattaggtcctggaactgtaatgtgcgataatatgagctatgttttcatgttcctttctattgcaacttccaatatcgtcgctacttcccttgcaaaaggggacaaaaatgaagtgcaacatcacatatccaccttgctctttgttgcattgacctgtggcttcttaatgcttttatttacaagattcttcggctcatgggcactgactgcttttactggatcgaagaacatagatattattccagctgcaaatacatacgttcagatacggagtttggcatggcctgcagttcttgttggttgggttacccagagtgcaagtcttggcatgaaagattcatggggtcctttgaaggccttagctgtagccagtgctattaatggccttggtgatatactactctgcagtgttttaggctatggtattgctggtgcagcatgggcgacaatgctgtcacagattgttgcaggatacatgatgattgaatctttgaacaagaaaggatacaactcttatgctatttctgttccctcacccaaagaacttctaacactaattgggcttgctgctcccgtgttggtgacaatgatgtctaaggttgctttctactcactcattgtatattttgcaacatctatgggcacatatacaatggctgctcatcaggtcatgattcaaacattcatgatctgtacggtgtggggcgaacctctctctcaaacggcacagtcatttatgcctgagttgatatatggagtaaatcgtagtttgcccaaggctcgaatgttgctcaagtcacttgtcatcattggagcaatccttggtttggctcttggaactgttggaacatctgttccatggttttttcctaacatctttacacctgatcagaagatcatacaggagatgcacaaactactgataccattttttttggcattggctgtgacacccgtcattctcagctttgagggaacattgctggctggacgagatcttagattcattagtctatcaatgagtagctgcttctctttaggtgcacttctgctgctgcttgtgagtagccaaggatgtggtttagtaggctgctggtgggtacttgtagcatttcaatgggctcggcttttcctttctttacgacgccttatatcacctgatggcatactctactctgaagatatgagccggtataaactggaagaagtcagagctgtatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

540

Amino Acids

58.33

Weight (kDa)

8.81

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 365, 853, 991
AciI CCGC 4 cut(s) 210, 239, 336, 399
AclWI GGATC 3 cut(s) 332, 338, 628
AcuI CTGAAG 1 cut(s) 1599
AcyI GRCGYC 2 cut(s) 169, 1546
AfaI GTAC 2 cut(s) 1096, 1503
AfiI CCNNNNNNNGG 3 cut(s) 320, 327, 1228
AgsI TTSAA 6 cut(s) 100, 754, 887, 895, 1079, 1517
AjnI CCWGG 1 cut(s) 409
AloI GAACNNNNNNTCC 4 cut(s) 1239, 1240, 1271, 1272
Alw21I GWGCWC 2 cut(s) 125, 1447
Alw26I GTCTC 2 cut(s) 62, 356
Alw44I GTGCAC 1 cut(s) 1443
AlwI GGATC 3 cut(s) 332, 338, 628
AoxI GGCC 5 cut(s) 311, 337, 683, 756, 784
ApaLI GTGCAC 1 cut(s) 1443
AseI ATTAAT 1 cut(s) 780
AspS9I GGNCC 4 cut(s) 311, 338, 407, 746
AsuC2I CCSGG 1 cut(s) 322
AsuHPI GGTGA 4 cut(s) 803, 927, 994, 1548
AvaII GGWCC 2 cut(s) 407, 746
BaeGI GKGCMC 3 cut(s) 604, 1046, 1447
BanII GRGCYC 2 cut(s) 125, 1525
Bbv12I GWGCWC 2 cut(s) 125, 1447
BbvCI CCTCAGC 1 cut(s) 154
BccI CCATC 2 cut(s) 200, 1556
BceAI ACGGC 2 cut(s) 156, 1140
BciT130I CCWGG 1 cut(s) 411
BciVI GTATCC 2 cut(s) 866, 899
BclI TGATCA 1 cut(s) 1288
BcnI CCSGG 1 cut(s) 322
BcoDI GTCTC 2 cut(s) 62, 356
BfaI CTAG 1 cut(s) 9
BfmI CTRYAG 3 cut(s) 687, 765, 805
BfuI GTATCC 2 cut(s) 866, 899
BglII AGATCT 1 cut(s) 1396
Bme1390I CCNGG 2 cut(s) 322, 411
Bme18I GGWCC 2 cut(s) 407, 746
BmgT120I GGNCC 4 cut(s) 311, 338, 407, 746
BmiI GGNNCC 1 cut(s) 747
BmrFI CCNGG 2 cut(s) 322, 411
BmrI ACTGGG 1 cut(s) 318
BmsI GCATC 1 cut(s) 1299
BmuI ACTGGG 1 cut(s) 318
BoxI GACNNNNGTC 1 cut(s) 1355
BpmI CTGGAG 1 cut(s) 249
Bpu10I CCTNAGC 2 cut(s) 154, 760
BpuEI CTTGAG 1 cut(s) 1178
BpuMI CCSGG 1 cut(s) 322
BsaBI GATNNNNATC 1 cut(s) 329
BsaHI GRCGYC 2 cut(s) 169, 1546
BsaJI CCNNGG 5 cut(s) 787, 1176, 1222, 1259, 1471
BsaXI ACNNNNNCTCC 2 cut(s) 58, 88
Bsc4I CCNNNNNNNGG 3 cut(s) 320, 327, 1228
Bse118I RCCGGY 2 cut(s) 313, 1590
Bse1I ACTGG 4 cut(s) 313, 622, 771, 1605
Bse3DI GCAATG 1 cut(s) 1379
Bse8I GATNNNNATC 1 cut(s) 329
BseBI CCWGG 1 cut(s) 411
BseDI CCNNGG 5 cut(s) 787, 1176, 1222, 1259, 1471
BseGI GGATG 2 cut(s) 149, 1481
BseJI GATNNNNATC 1 cut(s) 329
BseLI CCNNNNNNNGG 3 cut(s) 320, 327, 1228
BseMI GCAATG 1 cut(s) 1379
BseMII CTCAG 3 cut(s) 168, 1134, 1378
BseNI ACTGG 4 cut(s) 313, 622, 771, 1605
BseRI GAGGAG 4 cut(s) 63, 133, 136, 267
BseSI GKGCMC 3 cut(s) 604, 1046, 1447
BsgI GTGCAG 1 cut(s) 855
BshFI GGCC 5 cut(s) 313, 339, 685, 758, 786
BsiHKAI GWGCWC 2 cut(s) 125, 1447
BsiSI CCGG 3 cut(s) 314, 321, 1591
BslFI GGGAC 2 cut(s) 227, 515
BslI CCNNNNNNNGG 3 cut(s) 320, 327, 1228
BsmAI GTCTC 2 cut(s) 62, 356
BsmFI GGGAC 2 cut(s) 227, 515
BsnI GGCC 5 cut(s) 313, 339, 685, 758, 786
Bsp1286I GDGCHC 5 cut(s) 125, 604, 1046, 1447, 1525
Bsp143I GATC 7 cut(s) 324, 330, 620, 1089, 1288, 1296, 1396
Bsp19I CCATGG 1 cut(s) 1259
BspACI CCGC 4 cut(s) 210, 239, 336, 399
BspANI GGCC 5 cut(s) 313, 339, 685, 758, 786
BspCNI CTCAG 3 cut(s) 167, 1135, 1377
BspHI TCATGA 2 cut(s) 1071, 1086
BspLI GGNNCC 1 cut(s) 747
BspMAI CTGCAG 2 cut(s) 691, 809
BspPI GGATC 3 cut(s) 332, 338, 628
BsrDI GCAATG 1 cut(s) 1379
BsrFI RCCGGY 2 cut(s) 313, 1590
BsrI ACTGG 4 cut(s) 313, 622, 771, 1605
BssAI RCCGGY 2 cut(s) 313, 1590
BssECI CCNNGG 5 cut(s) 787, 1176, 1222, 1259, 1471
BssMI GATC 7 cut(s) 324, 330, 620, 1089, 1288, 1296, 1396
BssNI GRCGYC 2 cut(s) 169, 1546
BssT1I CCWWGG 5 cut(s) 787, 1176, 1222, 1259, 1471
Bst2UI CCWGG 1 cut(s) 411
Bst4CI ACNGT 5 cut(s) 364, 418, 1099, 1131, 1243
Bst6I CTCTTC 3 cut(s) 46, 65, 98
BstACI GRCGYC 2 cut(s) 169, 1546
BstAPI GCANNNNNTGC 2 cut(s) 608, 686
BstC8I GCNNGC 7 cut(s) 6, 196, 315, 396, 687, 966, 1386
BstDEI CTNAG 6 cut(s) 154, 760, 996, 1143, 1364, 1400
BstDSI CCRYGG 1 cut(s) 1259
BstEII GGTNACC 1 cut(s) 706
BstF5I GGATG 2 cut(s) 149, 1481
BstKTI GATC 7 cut(s) 327, 333, 623, 1092, 1291, 1299, 1399
BstMAI GTCTC 2 cut(s) 62, 356
BstMBI GATC 7 cut(s) 324, 330, 620, 1089, 1288, 1296, 1396
BstMWI GCNNNNNNNGC 5 cut(s) 135, 608, 686, 836, 842
BstNI CCWGG 1 cut(s) 411
BstPAI GACNNNNGTC 1 cut(s) 1355
BstPI GGTNACC 1 cut(s) 706
BstSCI CCNGG 2 cut(s) 320, 409
BstSFI CTRYAG 3 cut(s) 687, 765, 805
BstSLI GKGCMC 3 cut(s) 604, 1046, 1447
BstX2I RGATCY 2 cut(s) 324, 1396
BstXI CCANNNNNNTGG 1 cut(s) 1478
BstYI RGATCY 2 cut(s) 324, 1396
BsuI GTATCC 2 cut(s) 866, 899
BsuRI GGCC 5 cut(s) 313, 339, 685, 758, 786
BtgI CCRYGG 1 cut(s) 1259
BtgZI GCGATG 1 cut(s) 219
BtsCI GGATG 2 cut(s) 149, 1481
BtsI GCAGTG 1 cut(s) 814
BtsIMutI CAGTG 3 cut(s) 602, 778, 814
Cac8I GCNNGC 7 cut(s) 6, 196, 315, 396, 687, 966, 1386
CciI TCATGA 2 cut(s) 1071, 1086
Cfr10I RCCGGY 2 cut(s) 313, 1590
Cfr13I GGNCC 4 cut(s) 311, 338, 407, 746
CseI GACGC 2 cut(s) 177, 1554
Csp6I GTAC 2 cut(s) 1095, 1502
CviQI GTAC 2 cut(s) 1095, 1502
DdeI CTNAG 6 cut(s) 154, 760, 996, 1143, 1364, 1400
DpnI GATC 7 cut(s) 326, 332, 622, 1091, 1290, 1298, 1398
DpnII GATC 7 cut(s) 324, 330, 620, 1089, 1288, 1296, 1396
DrdI GACNNNNNNGTC 3 cut(s) 365, 853, 991
DseDI GACNNNNNNGTC 3 cut(s) 365, 853, 991
Eam1104I CTCTTC 3 cut(s) 46, 65, 98
EarI CTCTTC 3 cut(s) 46, 65, 98
Ecl136II GAGCTC 1 cut(s) 123
Eco130I CCWWGG 5 cut(s) 787, 1176, 1222, 1259, 1471
Eco147I AGGCCT 1 cut(s) 758
Eco24I GRGCYC 2 cut(s) 125, 1525
Eco47I GGWCC 2 cut(s) 407, 746
Eco53kI GAGCTC 1 cut(s) 123
Eco57I CTGAAG 1 cut(s) 1599
Eco91I GGTNACC 1 cut(s) 706
EcoICRI GAGCTC 1 cut(s) 123
EcoO109I RGGNCCY 2 cut(s) 407, 746
EcoO65I GGTNACC 1 cut(s) 706
EcoRII CCWGG 1 cut(s) 409
EcoT14I CCWWGG 5 cut(s) 787, 1176, 1222, 1259, 1471
EcoT38I GRGCYC 2 cut(s) 125, 1525
ErhI CCWWGG 5 cut(s) 787, 1176, 1222, 1259, 1471
FaqI GGGAC 2 cut(s) 227, 515
FauI CCCGC 1 cut(s) 203
FbaI TGATCA 1 cut(s) 1288
FokI GGATG 2 cut(s) 136, 1488
FriOI GRGCYC 2 cut(s) 125, 1525
FspBI CTAG 1 cut(s) 9
GsuI CTGGAG 1 cut(s) 249
HaeIII GGCC 5 cut(s) 313, 339, 685, 758, 786
HapII CCGG 3 cut(s) 314, 321, 1591
HgaI GACGC 2 cut(s) 177, 1554
Hin1I GRCGYC 2 cut(s) 169, 1546
HinfI GANTC 9 cut(s) 178, 265, 280, 349, 585, 737, 887, 1075, 1404
HpaII CCGG 3 cut(s) 314, 321, 1591
HphI GGTGA 4 cut(s) 803, 927, 994, 1548
Hpy166II GTNNAC 2 cut(s) 306, 1445
Hpy188I TCNGA 7 cut(s) 120, 270, 285, 666, 1293, 1579, 1613
Hpy188III TCNNGA 4 cut(s) 228, 328, 1072, 1087
Hpy8I GTNNAC 2 cut(s) 306, 1445
Hpy99I CGWCG 6 cut(s) 171, 368, 371, 374, 482, 1548
HpyAV CCTTC 3 cut(s) 90, 283, 748
HpyCH4III ACNGT 5 cut(s) 364, 418, 1099, 1131, 1243
HpyCH4IV ACGT 1 cut(s) 660
HpyF10VI GCNNNNNNNGC 5 cut(s) 135, 608, 686, 836, 842
HpyF3I CTNAG 6 cut(s) 154, 760, 996, 1143, 1364, 1400
HpySE526I ACGT 1 cut(s) 660
Hsp92I GRCGYC 2 cut(s) 169, 1546
KroI GCCGGC 1 cut(s) 313
KroNI GCCGGC 1 cut(s) 315
Ksp22I TGATCA 1 cut(s) 1288
Kzo9I GATC 7 cut(s) 324, 330, 620, 1089, 1288, 1296, 1396
LmnI GCTCC 4 cut(s) 163, 389, 976, 1214
LweI GCATC 1 cut(s) 1299
MaeI CTAG 1 cut(s) 9
MaeII ACGT 1 cut(s) 660
MaeIII GTNAC 5 cut(s) 706, 855, 982, 1197, 1348
MalI GATC 7 cut(s) 326, 332, 622, 1091, 1290, 1298, 1398
MboI GATC 7 cut(s) 324, 330, 620, 1089, 1288, 1296, 1396
MflI RGATCY 2 cut(s) 324, 1396
MhlI GDGCHC 5 cut(s) 125, 604, 1046, 1447, 1525
MluCI AATT 1 cut(s) 957
MlyI GAGTC 2 cut(s) 274, 358
MmeI TCCRAC 2 cut(s) 240, 1225
MroNI GCCGGC 1 cut(s) 313
MseI TTAA 3 cut(s) 246, 566, 780
MslI CAYNNNNRTG 1 cut(s) 1516
MspA1I CMGCKG 2 cut(s) 336, 647
MspI CCGG 3 cut(s) 314, 321, 1591
MspR9I CCNGG 2 cut(s) 322, 411
MvaI CCWGG 1 cut(s) 411
MwoI GCNNNNNNNGC 5 cut(s) 135, 608, 686, 836, 842
NaeI GCCGGC 1 cut(s) 315
NciI CCSGG 1 cut(s) 322
NcoI CCATGG 1 cut(s) 1259
NdeII GATC 7 cut(s) 324, 330, 620, 1089, 1288, 1296, 1396
NgoMIV GCCGGC 1 cut(s) 313
NlaIV GGNNCC 1 cut(s) 747
NmeAIII GCCGAG 1 cut(s) 1504
NmuCI GTSAC 4 cut(s) 855, 982, 1197, 1348
PagI TCATGA 2 cut(s) 1071, 1086
PceI AGGCCT 1 cut(s) 758
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PdiI GCCGGC 1 cut(s) 315
PfeI GAWTC 7 cut(s) 178, 280, 585, 737, 887, 1075, 1404
PflFI GACNNNGTC 1 cut(s) 362
PfoI TCCNGGA 1 cut(s) 409
PleI GAGTC 2 cut(s) 273, 357
PpsI GAGTC 2 cut(s) 273, 357
PpuMI RGGWCCY 2 cut(s) 407, 746
PshAI GACNNNNGTC 1 cut(s) 1355
PshBI ATTAAT 1 cut(s) 780
Psp124BI GAGCTC 1 cut(s) 125
Psp5II RGGWCCY 2 cut(s) 407, 746
Psp6I CCWGG 1 cut(s) 409
PspEI GGTNACC 1 cut(s) 706
PspGI CCWGG 1 cut(s) 409
PspN4I GGNNCC 1 cut(s) 747
PspPI GGNCC 4 cut(s) 311, 338, 407, 746
PspPPI RGGWCCY 2 cut(s) 407, 746
PstI CTGCAG 2 cut(s) 691, 809
PsuI RGATCY 2 cut(s) 324, 1396
PsyI GACNNNGTC 1 cut(s) 362
PvuII CAGCTG 1 cut(s) 647
RsaI GTAC 2 cut(s) 1096, 1503
RsaNI GTAC 2 cut(s) 1095, 1502
RseI CAYNNNNRTG 1 cut(s) 1516
SacI GAGCTC 1 cut(s) 125
SaqAI TTAA 3 cut(s) 246, 566, 780
Sau3AI GATC 7 cut(s) 324, 330, 620, 1089, 1288, 1296, 1396
Sau96I GGNCC 4 cut(s) 311, 338, 407, 746
SchI GAGTC 2 cut(s) 274, 358
ScrFI CCNGG 2 cut(s) 322, 411
SduI GDGCHC 5 cut(s) 125, 604, 1046, 1447, 1525
SfaNI GCATC 1 cut(s) 1299
SfcI CTRYAG 3 cut(s) 687, 765, 805
SinI GGWCC 2 cut(s) 407, 746
SmiMI CAYNNNNRTG 1 cut(s) 1516
SmlI CTYRAG 1 cut(s) 1193
SmoI CTYRAG 1 cut(s) 1193
Sse9I AATT 1 cut(s) 957
SseBI AGGCCT 1 cut(s) 758
SsiI CCGC 4 cut(s) 210, 239, 336, 399
SspMI CTAG 1 cut(s) 9
SstI GAGCTC 1 cut(s) 125
StuI AGGCCT 1 cut(s) 758
StyD4I CCNGG 2 cut(s) 320, 409
StyI CCWWGG 5 cut(s) 787, 1176, 1222, 1259, 1471
TaaI ACNGT 5 cut(s) 364, 418, 1099, 1131, 1243
TaiI ACGT 1 cut(s) 663
TaqI TCGA 5 cut(s) 112, 176, 357, 623, 1183
TaqII GACCGA 1 cut(s) 362
TasI AATT 1 cut(s) 957
TauI GCSGC 2 cut(s) 339, 401
TfiI GAWTC 7 cut(s) 178, 280, 585, 737, 887, 1075, 1404
Tru1I TTAA 3 cut(s) 246, 566, 780
Tru9I TTAA 3 cut(s) 246, 566, 780
TscAI CASTG 3 cut(s) 609, 778, 814
TseFI GTSAC 4 cut(s) 855, 982, 1197, 1348
Tsp45I GTSAC 4 cut(s) 855, 982, 1197, 1348
TspDTI ATGAA 7 cut(s) 302, 436, 525, 729, 746, 1075, 1396
TspGWI ACGGA 1 cut(s) 685
TspRI CASTG 3 cut(s) 609, 778, 814
Tth111I GACNNNGTC 1 cut(s) 362
VneI GTGCAC 1 cut(s) 1443
VpaK11BI GGWCC 2 cut(s) 407, 746
VspI ATTAAT 1 cut(s) 780
XspI CTAG 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.