RLG00000014030

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001733
Physical Location & Seq
Forward (+)
45956293 .. 45963112
6820 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014030

Sequence Viewer

Length: 1467 bp
ATGCAGACCAAAACCCTAACAGCTCACCCTCCTCACCCCCGTCTCCTTCCAAACCCTAACCGCCACAACTCCCTTCCCCAACCTTCGTTCTCACCCTTCTCTTTCACGCCGCGCTTGAAAAACACGCATCAACTTCCAATATTGCGCTCCTCGGCGCCAATACGGCGTCGTAACAGGCTATTAACAGCTTGTGTTAGCGAAAACCTCGATGTAGGAAACGGCGACGCTTTGGAGGTGGTTAAGGTTGAGGAATTGGAGAGTCGGAGCATATGGAATCAGATGAAGGAGATTGCAATGTTCACTGGACCCGCTACTGGGCTTTGGTTGTGCGGCCCACTTATGAGTCTGATTGACACTGTCGTCATCGGTCAGGGAAGCACAATCGAGCTTGCTGCTTTAGGCCCTGGAACTGTAATGTGTGACTATATGAGTTATGTGTTCATGTTCCTTTCTATTGCAACTTCAAACATGGTTGCCACTTCGCTTGCGAGACGGGACAAGAATGAAGTGCAACATCAGATATCTATCTTGCTTTTTCTTGGATTGGCATCTGGCTTCTTAATGCTTTTATTTACAAGATTCTTCGGTTCATGGGCACTAACTGATACGAGGATTCGCATGGCCGGCACTCCTTGTTGGTTGGGTTGCTCAGAGTGCAAGCCTTGGCATGAAAGATTCTTGGGCTATTTAGGCTATGGTATAGCTGGTGCAGCATGGGCAACTATGGTTTCACAGGTTGTTGCAGGATATATGATGATTGAAGCTCTGAACAAAAAAGGTTACGATGGCTATGCCATTTCTGTTCCATCGTCCAAGGAACTTCTAACAGTACTTGGGCTTGCTGCTCCAGTATTTATGACATTGATGTCAAAGATAGTTTTCTACTCTCTCCTTATCTATTTTGCGACGTCTATGGGAACACATACCATGGCTGCTCATCAGGTCATGATACAAACATTCTGCATGTGTACAGTGTGGGGTGAGCCTCTCTCTCAAACTGCACAATCGTTTATGCCGGAATTTTTATTTGGAGTGAATCATAGTTTGCCTAAGGCCCGCACATTGCTCAAGTCACTTGTCATCATTGGAGCTATACTTGGTTCAGTATTAGGAATAGTTGGAACATGTGTTCCATGGTTCTTTCCCGGTATCTTTGCACCTGATCAAAAGATCATACAAGAGATGCATAAGGTGCTAATACCATATTTCCTTGCATTAGCTGTAACGCCCCCGACTCACTATCTGAGATTTATTAGTCTGTCAATGAGTGGATGCTTCTCTTTGGGTGCACTTCTAGTGCTGCTTGTGAGCAGTAGAGGATTTGGTTTAGCAGGCGTCTGGTGGGCATTAGCAGGATTTCAATGGAGTCGGCTTTTACTCTCTCTACGCCGCCTTATATCCTCGGATGGGATACTCTACTCTGAAGATGTGAGCCGGTATAAACTGGAAAAGCTCAGAGCTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

489

Amino Acids

53.93

Weight (kDa)

9.26

Isoelectric Point (pI)

45.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 112 - 199 1.6e-06 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 359, 865
AatII GACGTC 1 cut(s) 911
AccB1I GGYRCC 1 cut(s) 154
AccII CGCG 1 cut(s) 112
AciI CCGC 6 cut(s) 61, 110, 309, 330, 1057, 1390
AcoI YGGCCR 1 cut(s) 621
AcsI RAATTY 1 cut(s) 1019
AcuI CTGAAG 1 cut(s) 1443
AcyI GRCGYC 4 cut(s) 155, 166, 908, 1335
AfaI GTAC 2 cut(s) 831, 970
AfiI CCNNNNNNNGG 3 cut(s) 314, 315, 1407
AflIII ACRYGT 1 cut(s) 1124
AgsI TTSAA 4 cut(s) 118, 465, 761, 1361
AjnI CCWGG 1 cut(s) 403
AjuI GAANNNNNNNTTGG 2 cut(s) 1011, 1043
AloI GAACNNNNNNTCC 4 cut(s) 1113, 1114, 1145, 1146
AluBI AGCT 9 cut(s) 23, 188, 388, 704, 764, 1091, 1220, 1453, 1460
AluI AGCT 9 cut(s) 23, 188, 388, 704, 764, 1091, 1220, 1453, 1460
Alw21I GWGCWC 1 cut(s) 1291
Alw26I GTCTC 2 cut(s) 47, 484
Alw44I GTGCAC 1 cut(s) 1287
AoxI GGCC 4 cut(s) 331, 400, 621, 1053
ApaLI GTGCAC 1 cut(s) 1287
ApeKI GCWGC 5 cut(s) 392, 710, 842, 932, 1300
ApoI RAATTY 1 cut(s) 1019
AspLEI GCGC 3 cut(s) 114, 147, 157
AspS9I GGNCC 4 cut(s) 305, 332, 401, 1054
AsuC2I CCSGG 1 cut(s) 1146
AsuHPI GGTGA 4 cut(s) 17, 26, 84, 992
AvaII GGWCC 1 cut(s) 305
AxyI CCTNAGG 1 cut(s) 1050
BaeGI GKGCMC 2 cut(s) 598, 1291
BanI GGYRCC 1 cut(s) 154
Bbv12I GWGCWC 1 cut(s) 1291
BbvI GCAGC 5 cut(s) 379, 722, 829, 919, 1287
BccI CCATC 3 cut(s) 779, 814, 1400
BceAI ACGGC 2 cut(s) 179, 235
BcgI CGANNNNNNTGC 4 cut(s) 374, 408, 773, 807
BciT130I CCWGG 1 cut(s) 405
BciVI GTATCC 1 cut(s) 1404
BclI TGATCA 1 cut(s) 1162
BcnI CCSGG 1 cut(s) 1146
BcoDI GTCTC 2 cut(s) 47, 484
BfaI CTAG 1 cut(s) 1295
BfoI RGCGCY 1 cut(s) 158
BfuI GTATCC 1 cut(s) 1404
BglI GCCNNNNNGGC 1 cut(s) 163
BisI GCNGC 8 cut(s) 110, 331, 393, 711, 843, 933, 1301, 1390
BlsI GCNGC 8 cut(s) 111, 332, 394, 712, 844, 934, 1302, 1391
BmcAI AGTACT 1 cut(s) 831
Bme1390I CCNGG 2 cut(s) 405, 1146
Bme18I GGWCC 1 cut(s) 305
BmgT120I GGNCC 4 cut(s) 305, 332, 401, 1054
BmiI GGNNCC 2 cut(s) 156, 307
BmrFI CCNGG 2 cut(s) 405, 1146
BmrI ACTGGG 1 cut(s) 324
BmsI GCATC 4 cut(s) 136, 557, 1173, 1262
BmuI ACTGGG 1 cut(s) 324
BplI GAGNNNNNCTC 2 cut(s) 974, 1006
BpmI CTGGAG 1 cut(s) 831
BpuEI CTTGAG 1 cut(s) 1052
BpuMI CCSGG 1 cut(s) 1146
BsaBI GATNNNNATC 2 cut(s) 524, 547
BsaHI GRCGYC 4 cut(s) 155, 166, 908, 1335
BsaJI CCNNGG 7 cut(s) 150, 403, 662, 813, 927, 1133, 1401
Bsc4I CCNNNNNNNGG 3 cut(s) 314, 315, 1407
Bse118I RCCGGY 2 cut(s) 623, 1434
Bse1I ACTGG 4 cut(s) 307, 319, 848, 1449
Bse21I CCTNAGG 1 cut(s) 1050
Bse3DI GCAATG 2 cut(s) 300, 1061
Bse8I GATNNNNATC 2 cut(s) 524, 547
BseBI CCWGG 1 cut(s) 405
BseDI CCNNGG 7 cut(s) 150, 403, 662, 813, 927, 1133, 1401
BseGI GGATG 2 cut(s) 1277, 1411
BseJI GATNNNNATC 2 cut(s) 524, 547
BseLI CCNNNNNNNGG 3 cut(s) 314, 315, 1407
BseMI GCAATG 2 cut(s) 300, 1061
BseMII CTCAG 2 cut(s) 663, 1235
BseNI ACTGG 4 cut(s) 307, 319, 848, 1449
BseRI GAGGAG 2 cut(s) 21, 139
BseSI GKGCMC 2 cut(s) 598, 1291
BseXI GCAGC 5 cut(s) 379, 722, 829, 919, 1287
BsgI GTGCAG 2 cut(s) 729, 984
Bsh1236I CGCG 1 cut(s) 112
BshFI GGCC 4 cut(s) 333, 402, 623, 1055
BshNI GGYRCC 1 cut(s) 154
BsiHKAI GWGCWC 1 cut(s) 1291
BsiSI CCGG 4 cut(s) 624, 1016, 1146, 1435
BslFI GGGAC 1 cut(s) 509
BslI CCNNNNNNNGG 3 cut(s) 314, 315, 1407
BsmAI GTCTC 2 cut(s) 47, 484
BsmBI CGTCTC 2 cut(s) 47, 484
BsmFI GGGAC 1 cut(s) 509
BsnI GGCC 4 cut(s) 333, 402, 623, 1055
Bsp1286I GDGCHC 2 cut(s) 598, 1291
Bsp1407I TGTACA 1 cut(s) 968
Bsp143I GATC 2 cut(s) 1162, 1170
Bsp19I CCATGG 2 cut(s) 927, 1133
BspACI CCGC 6 cut(s) 61, 110, 309, 330, 1057, 1390
BspANI GGCC 4 cut(s) 333, 402, 623, 1055
BspCNI CTCAG 3 cut(s) 662, 1236, 1467
BspFNI CGCG 1 cut(s) 112
BspHI TCATGA 1 cut(s) 945
BspLI GGNNCC 2 cut(s) 156, 307
BspT107I GGYRCC 1 cut(s) 154
BsrDI GCAATG 2 cut(s) 300, 1061
BsrFI RCCGGY 2 cut(s) 623, 1434
BsrGI TGTACA 1 cut(s) 968
BsrI ACTGG 4 cut(s) 307, 319, 848, 1449
BssAI RCCGGY 2 cut(s) 623, 1434
BssECI CCNNGG 7 cut(s) 150, 403, 662, 813, 927, 1133, 1401
BssMI GATC 2 cut(s) 1162, 1170
BssNI GRCGYC 4 cut(s) 155, 166, 908, 1335
BssT1I CCWWGG 4 cut(s) 662, 813, 927, 1133
Bst2UI CCWGG 1 cut(s) 405
Bst4CI ACNGT 4 cut(s) 358, 412, 829, 973
BstACI GRCGYC 4 cut(s) 155, 166, 908, 1335
BstAPI GCANNNNNTGC 1 cut(s) 1192
BstAUI TGTACA 1 cut(s) 968
BstC8I GCNNGC 7 cut(s) 390, 486, 625, 659, 840, 1057, 1333
BstDEI CTNAG 4 cut(s) 649, 1050, 1244, 1454
BstDSI CCRYGG 2 cut(s) 927, 1133
BstF5I GGATG 2 cut(s) 1277, 1411
BstFNI CGCG 1 cut(s) 112
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 3 cut(s) 114, 147, 157
BstKTI GATC 2 cut(s) 1165, 1173
BstMAI GTCTC 2 cut(s) 47, 484
BstMBI GATC 2 cut(s) 1162, 1170
BstMWI GCNNNNNNNGC 7 cut(s) 163, 624, 654, 690, 710, 716, 1192
BstNI CCWGG 1 cut(s) 405
BstNSI RCATGY 2 cut(s) 967, 1128
BstSCI CCNGG 2 cut(s) 403, 1144
BstSLI GKGCMC 2 cut(s) 598, 1291
BstUI CGCG 1 cut(s) 112
BstV1I GCAGC 5 cut(s) 379, 722, 829, 919, 1287
Bsu36I CCTNAGG 1 cut(s) 1050
BsuI GTATCC 1 cut(s) 1404
BsuRI GGCC 4 cut(s) 333, 402, 623, 1055
BtgI CCRYGG 2 cut(s) 927, 1133
BtsCI GGATG 2 cut(s) 1277, 1411
BtsIMutI CAGTG 3 cut(s) 300, 354, 978
Cac8I GCNNGC 7 cut(s) 390, 486, 625, 659, 840, 1057, 1333
CciI TCATGA 1 cut(s) 945
CfoI GCGC 3 cut(s) 114, 147, 157
Cfr10I RCCGGY 2 cut(s) 623, 1434
Cfr13I GGNCC 4 cut(s) 305, 332, 401, 1054
CseI GACGC 3 cut(s) 155, 233, 1324
Csp6I GTAC 2 cut(s) 830, 969
CspCI CAANNNNNGTGG 2 cut(s) 466, 501
CviQI GTAC 2 cut(s) 830, 969
DdeI CTNAG 4 cut(s) 649, 1050, 1244, 1454
DinI GGCGCC 1 cut(s) 156
DpnI GATC 2 cut(s) 1164, 1172
DpnII GATC 2 cut(s) 1162, 1170
DrdI GACNNNNNNGTC 2 cut(s) 359, 865
DseDI GACNNNNNNGTC 2 cut(s) 359, 865
EaeI YGGCCR 1 cut(s) 621
Eco130I CCWWGG 4 cut(s) 662, 813, 927, 1133
Eco32I GATATC 1 cut(s) 522
Eco47I GGWCC 1 cut(s) 305
Eco57I CTGAAG 1 cut(s) 1443
Eco81I CCTNAGG 1 cut(s) 1050
EcoO109I RGGNCCY 1 cut(s) 401
EcoRII CCWGG 1 cut(s) 403
EcoRV GATATC 1 cut(s) 522
EcoT14I CCWWGG 4 cut(s) 662, 813, 927, 1133
EcoT22I ATGCAT 1 cut(s) 1188
EgeI GGCGCC 1 cut(s) 156
EheI GGCGCC 1 cut(s) 156
ErhI CCWWGG 4 cut(s) 662, 813, 927, 1133
Esp3I CGTCTC 2 cut(s) 47, 484
FaqI GGGAC 1 cut(s) 509
FauI CCCGC 2 cut(s) 316, 1064
FauNDI CATATG 1 cut(s) 269
FbaI TGATCA 1 cut(s) 1162
Fnu4HI GCNGC 8 cut(s) 110, 331, 393, 711, 843, 933, 1301, 1390
FokI GGATG 2 cut(s) 1284, 1418
Fsp4HI GCNGC 8 cut(s) 110, 331, 393, 711, 843, 933, 1301, 1390
FspBI CTAG 1 cut(s) 1295
GlaI GCGC 3 cut(s) 113, 146, 156
GluI GCNGC 8 cut(s) 110, 331, 393, 711, 843, 933, 1301, 1390
GsuI CTGGAG 1 cut(s) 831
HaeII RGCGCY 1 cut(s) 158
HaeIII GGCC 4 cut(s) 333, 402, 623, 1055
HapII CCGG 4 cut(s) 624, 1016, 1146, 1435
HgaI GACGC 3 cut(s) 155, 233, 1324
HhaI GCGC 3 cut(s) 114, 147, 157
Hin1I GRCGYC 4 cut(s) 155, 166, 908, 1335
Hin6I GCGC 3 cut(s) 112, 145, 155
HinP1I GCGC 3 cut(s) 112, 145, 155
HinfI GANTC 9 cut(s) 259, 274, 343, 579, 613, 675, 1036, 1234, 1366
HpaII CCGG 4 cut(s) 624, 1016, 1146, 1435
HphI GGTGA 4 cut(s) 17, 26, 84, 992
Hpy166II GTNNAC 3 cut(s) 300, 969, 1289
Hpy188III TCNNGA 1 cut(s) 946
Hpy8I GTNNAC 3 cut(s) 300, 969, 1289
Hpy99I CGWCG 3 cut(s) 171, 227, 910
HpyAV CCTTC 5 cut(s) 56, 83, 93, 106, 277
HpyCH4III ACNGT 4 cut(s) 358, 412, 829, 973
HpyCH4IV ACGT 1 cut(s) 908
HpyF10VI GCNNNNNNNGC 7 cut(s) 163, 624, 654, 690, 710, 716, 1192
HpyF3I CTNAG 4 cut(s) 649, 1050, 1244, 1454
HpySE526I ACGT 1 cut(s) 908
Hsp92I GRCGYC 4 cut(s) 155, 166, 908, 1335
HspAI GCGC 3 cut(s) 112, 145, 155
KasI GGCGCC 1 cut(s) 154
KroI GCCGGC 1 cut(s) 623
KroNI GCCGGC 1 cut(s) 625
Ksp22I TGATCA 1 cut(s) 1162
Kzo9I GATC 2 cut(s) 1162, 1170
LmnI GCTCC 4 cut(s) 152, 264, 850, 1088
Lsp1109I GCAGC 5 cut(s) 379, 722, 829, 919, 1287
LweI GCATC 4 cut(s) 136, 557, 1173, 1262
MaeI CTAG 1 cut(s) 1295
MaeII ACGT 1 cut(s) 908
MaeIII GTNAC 5 cut(s) 170, 419, 779, 1071, 1222
MalI GATC 2 cut(s) 1164, 1172
MboI GATC 2 cut(s) 1162, 1170
MboII GAAGA 2 cut(s) 574, 1436
MhlI GDGCHC 2 cut(s) 598, 1291
MluCI AATT 2 cut(s) 251, 1019
Mly113I GGCGCC 1 cut(s) 155
MlyI GAGTC 4 cut(s) 268, 352, 1228, 1375
MmeI TCCRAC 2 cut(s) 242, 1099
Mph1103I ATGCAT 1 cut(s) 1188
MroNI GCCGGC 1 cut(s) 623
MseI TTAA 3 cut(s) 182, 240, 560
MspI CCGG 4 cut(s) 624, 1016, 1146, 1435
MspR9I CCNGG 2 cut(s) 405, 1146
MvaI CCWGG 1 cut(s) 405
MvnI CGCG 1 cut(s) 112
MwoI GCNNNNNNNGC 7 cut(s) 163, 624, 654, 690, 710, 716, 1192
NaeI GCCGGC 1 cut(s) 625
NarI GGCGCC 1 cut(s) 155
NciI CCSGG 1 cut(s) 1146
NcoI CCATGG 2 cut(s) 927, 1133
NdeI CATATG 1 cut(s) 269
NdeII GATC 2 cut(s) 1162, 1170
NgoMIV GCCGGC 1 cut(s) 623
NlaIV GGNNCC 2 cut(s) 156, 307
NmeAIII GCCGAG 1 cut(s) 131
NmuCI GTSAC 2 cut(s) 419, 1071
NsiI ATGCAT 1 cut(s) 1188
NspI RCATGY 2 cut(s) 967, 1128
PagI TCATGA 1 cut(s) 945
PciI ACATGT 1 cut(s) 1124
PdiI GCCGGC 1 cut(s) 625
PfeI GAWTC 5 cut(s) 274, 579, 613, 675, 1036
PflFI GACNNNGTC 1 cut(s) 356
PkrI GCNGC 8 cut(s) 111, 332, 394, 712, 844, 934, 1302, 1391
PleI GAGTC 4 cut(s) 267, 351, 1228, 1374
PluTI GGCGCC 1 cut(s) 158
PpsI GAGTC 4 cut(s) 267, 351, 1228, 1374
PscI ACATGT 1 cut(s) 1124
Psp6I CCWGG 1 cut(s) 403
PspGI CCWGG 1 cut(s) 403
PspN4I GGNNCC 2 cut(s) 156, 307
PspPI GGNCC 4 cut(s) 305, 332, 401, 1054
PsyI GACNNNGTC 1 cut(s) 356
RsaI GTAC 2 cut(s) 831, 970
RsaNI GTAC 2 cut(s) 830, 969
SaqAI TTAA 3 cut(s) 182, 240, 560
SatI GCNGC 8 cut(s) 110, 331, 393, 711, 843, 933, 1301, 1390
Sau3AI GATC 2 cut(s) 1162, 1170
Sau96I GGNCC 4 cut(s) 305, 332, 401, 1054
ScaI AGTACT 1 cut(s) 831
SchI GAGTC 4 cut(s) 268, 352, 1228, 1375
ScrFI CCNGG 2 cut(s) 405, 1146
SduI GDGCHC 2 cut(s) 598, 1291
SfaNI GCATC 4 cut(s) 136, 557, 1173, 1262
SfoI GGCGCC 1 cut(s) 156
SinI GGWCC 1 cut(s) 305
SmlI CTYRAG 1 cut(s) 1067
SmoI CTYRAG 1 cut(s) 1067
Sse9I AATT 2 cut(s) 251, 1019
SsiI CCGC 6 cut(s) 61, 110, 309, 330, 1057, 1390
SspDI GGCGCC 1 cut(s) 154
SspI AATATT 1 cut(s) 141
SspMI CTAG 1 cut(s) 1295
StyD4I CCNGG 2 cut(s) 403, 1144
StyI CCWWGG 4 cut(s) 662, 813, 927, 1133
TaaI ACNGT 4 cut(s) 358, 412, 829, 973
TaiI ACGT 1 cut(s) 911
TaqI TCGA 2 cut(s) 207, 384
TaqII GACCGA 1 cut(s) 356
TasI AATT 2 cut(s) 251, 1019
TatI WGTACW 2 cut(s) 829, 968
TauI GCSGC 3 cut(s) 112, 333, 1392
TfiI GAWTC 5 cut(s) 274, 579, 613, 675, 1036
Tru1I TTAA 3 cut(s) 182, 240, 560
Tru9I TTAA 3 cut(s) 182, 240, 560
TscAI CASTG 3 cut(s) 307, 361, 978
TseFI GTSAC 2 cut(s) 419, 1071
TseI GCWGC 5 cut(s) 392, 710, 842, 932, 1300
Tsp45I GTSAC 2 cut(s) 419, 1071
TspDTI ATGAA 5 cut(s) 296, 430, 519, 579, 684
TspRI CASTG 3 cut(s) 307, 361, 978
Tth111I GACNNNGTC 1 cut(s) 356
VneI GTGCAC 1 cut(s) 1287
VpaK11BI GGWCC 1 cut(s) 305
XapI RAATTY 1 cut(s) 1019
XceI RCATGY 2 cut(s) 967, 1128
XspI CTAG 1 cut(s) 1295
ZraI GACGTC 1 cut(s) 909
ZrmI AGTACT 1 cut(s) 831
Zsp2I ATGCAT 1 cut(s) 1188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.