RLG00000014027

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001733
Physical Location & Seq
Forward (+)
45919019 .. 45920220
1202 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014027

Sequence Viewer

Length: 522 bp
ATGAAAGATTCATGGGGTCCTTTGAAGGCCTTAGCTGTAGCCAGTGCTATTAATGCCCTTGGTGATATACTACTCTGCAGCGTTTTAGGCTATGGTATTGCTGGTGCAGCATGGGCGACAATGCTGTCACAGATTGTTGCAGGATACATGATGATTGAATCTTTGAACAAGAAAGGATACAACTCTTATGCTATTTCTGTTCCCTCACCCAAAGAACTTCTAACACTAATTGGGCTTGCTGCTCCAGTGTTGGTGACAATGATGTGTAAGGTTGCTTTCTACTTGCTCATTGTATATTTTGCAACATCTATGGGCACATATACAATGGCTGCTCATCAGGCTCGAATGTTGCTCAAGTCACTTGTCATCATTGGAGCAATCCTTGGTTTGGCTCTTGGAACTGTTGGAACATCTGTTCCATGGTTTTTTCCTAACATCTTTACACCTGATCAGAAGATCATACAGGAGGTTGGTTCTTTCTGTTGTGAGCTTTTCATAATCTTCCTAATTTCTACTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

174

Amino Acids

18.51

Weight (kDa)

8.34

Isoelectric Point (pI)

25.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 124
AfiI CCNNNNNNNGG 1 cut(s) 388
AgsI TTSAA 3 cut(s) 25, 158, 166
AloI GAACNNNNNNTCC 4 cut(s) 399, 400, 431, 432
AluBI AGCT 2 cut(s) 35, 490
AluI AGCT 2 cut(s) 35, 490
AoxI GGCC 1 cut(s) 27
ApeKI GCWGC 4 cut(s) 78, 107, 239, 329
AseI ATTAAT 1 cut(s) 51
AspS9I GGNCC 1 cut(s) 17
AsuHPI GGTGA 3 cut(s) 74, 198, 265
AvaII GGWCC 1 cut(s) 17
BaeGI GKGCMC 1 cut(s) 317
BbvI GCAGC 4 cut(s) 90, 119, 226, 316
BciVI GTATCC 2 cut(s) 137, 170
BclI TGATCA 1 cut(s) 448
BfmI CTRYAG 3 cut(s) 36, 76, 518
BfuI GTATCC 2 cut(s) 137, 170
BisI GCNGC 4 cut(s) 79, 108, 240, 330
BlsI GCNGC 4 cut(s) 80, 109, 241, 331
Bme18I GGWCC 1 cut(s) 17
BmgT120I GGNCC 1 cut(s) 17
BmiI GGNNCC 1 cut(s) 18
BpmI CTGGAG 1 cut(s) 228
Bpu10I CCTNAGC 1 cut(s) 31
BpuEI CTTGAG 1 cut(s) 338
BsaJI CCNNGG 3 cut(s) 58, 382, 419
Bsc4I CCNNNNNNNGG 1 cut(s) 388
Bse1I ACTGG 2 cut(s) 42, 245
BseDI CCNNGG 3 cut(s) 58, 382, 419
BseLI CCNNNNNNNGG 1 cut(s) 388
BseNI ACTGG 2 cut(s) 42, 245
BseSI GKGCMC 1 cut(s) 317
BseXI GCAGC 4 cut(s) 90, 119, 226, 316
BsgI GTGCAG 1 cut(s) 126
BshFI GGCC 1 cut(s) 29
BslI CCNNNNNNNGG 1 cut(s) 388
BsnI GGCC 1 cut(s) 29
Bsp1286I GDGCHC 1 cut(s) 317
Bsp143I GATC 2 cut(s) 448, 456
Bsp19I CCATGG 1 cut(s) 419
BspANI GGCC 1 cut(s) 29
BspLI GGNNCC 1 cut(s) 18
BspMAI CTGCAG 1 cut(s) 80
BsrI ACTGG 2 cut(s) 42, 245
BssECI CCNNGG 3 cut(s) 58, 382, 419
BssMI GATC 2 cut(s) 448, 456
BssT1I CCWWGG 3 cut(s) 58, 382, 419
Bst4CI ACNGT 1 cut(s) 403
BstC8I GCNNGC 1 cut(s) 237
BstDEI CTNAG 1 cut(s) 31
BstDSI CCRYGG 1 cut(s) 419
BstKTI GATC 2 cut(s) 451, 459
BstMBI GATC 2 cut(s) 448, 456
BstMWI GCNNNNNNNGC 5 cut(s) 53, 87, 107, 113, 338
BstSFI CTRYAG 3 cut(s) 36, 76, 518
BstSLI GKGCMC 1 cut(s) 317
BstV1I GCAGC 4 cut(s) 90, 119, 226, 316
BsuI GTATCC 2 cut(s) 137, 170
BsuRI GGCC 1 cut(s) 29
BtgI CCRYGG 1 cut(s) 419
BtsIMutI CAGTG 2 cut(s) 49, 252
Cac8I GCNNGC 1 cut(s) 237
Cfr13I GGNCC 1 cut(s) 17
CviAII CATG 4 cut(s) 12, 111, 148, 420
CviJI RGCY 9 cut(s) 29, 35, 41, 90, 235, 329, 341, 392, 490
CviKI_1 RGCY 9 cut(s) 29, 35, 41, 90, 235, 329, 341, 392, 490
DdeI CTNAG 1 cut(s) 31
DpnI GATC 2 cut(s) 450, 458
DpnII GATC 2 cut(s) 448, 456
DrdI GACNNNNNNGTC 1 cut(s) 124
DseDI GACNNNNNNGTC 1 cut(s) 124
Eco130I CCWWGG 3 cut(s) 58, 382, 419
Eco147I AGGCCT 1 cut(s) 29
Eco47I GGWCC 1 cut(s) 17
EcoO109I RGGNCCY 1 cut(s) 17
EcoT14I CCWWGG 3 cut(s) 58, 382, 419
ErhI CCWWGG 3 cut(s) 58, 382, 419
FaeI CATG 4 cut(s) 15, 114, 151, 423
FatI CATG 4 cut(s) 11, 110, 147, 419
FbaI TGATCA 1 cut(s) 448
Fnu4HI GCNGC 4 cut(s) 79, 108, 240, 330
Fsp4HI GCNGC 4 cut(s) 79, 108, 240, 330
GluI GCNGC 4 cut(s) 79, 108, 240, 330
GsuI CTGGAG 1 cut(s) 228
HaeIII GGCC 1 cut(s) 29
Hin1II CATG 4 cut(s) 15, 114, 151, 423
HinfI GANTC 2 cut(s) 8, 158
HphI GGTGA 3 cut(s) 74, 198, 265
Hpy188I TCNGA 1 cut(s) 453
HpyAV CCTTC 1 cut(s) 19
HpyCH4III ACNGT 1 cut(s) 403
HpyCH4V TGCA 4 cut(s) 78, 107, 140, 302
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 87, 107, 113, 338
HpyF3I CTNAG 1 cut(s) 31
Hsp92II CATG 4 cut(s) 15, 114, 151, 423
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 2 cut(s) 448, 456
LmnI GCTCC 2 cut(s) 247, 374
LpnPI CCDG 7 cut(s) 55, 87, 126, 258, 323, 449, 459
Lsp1109I GCAGC 4 cut(s) 90, 119, 226, 316
MaeIII GTNAC 3 cut(s) 126, 253, 357
MalI GATC 2 cut(s) 450, 458
MboI GATC 2 cut(s) 448, 456
MboII GAAGA 2 cut(s) 466, 493
MhlI GDGCHC 1 cut(s) 317
MluCI AATT 2 cut(s) 228, 507
MmeI TCCRAC 1 cut(s) 385
MnlI CCTC 2 cut(s) 214, 460
MseI TTAA 1 cut(s) 51
MwoI GCNNNNNNNGC 5 cut(s) 53, 87, 107, 113, 338
NcoI CCATGG 1 cut(s) 419
NdeII GATC 2 cut(s) 448, 456
NlaIII CATG 4 cut(s) 15, 114, 151, 423
NlaIV GGNNCC 1 cut(s) 18
NmuCI GTSAC 3 cut(s) 126, 253, 357
PceI AGGCCT 1 cut(s) 29
PfeI GAWTC 2 cut(s) 8, 158
PkrI GCNGC 4 cut(s) 80, 109, 241, 331
PpuMI RGGWCCY 1 cut(s) 17
PshBI ATTAAT 1 cut(s) 51
Psp5II RGGWCCY 1 cut(s) 17
PspN4I GGNNCC 1 cut(s) 18
PspPI GGNCC 1 cut(s) 17
PspPPI RGGWCCY 1 cut(s) 17
PstI CTGCAG 1 cut(s) 80
SaqAI TTAA 1 cut(s) 51
SatI GCNGC 4 cut(s) 79, 108, 240, 330
Sau3AI GATC 2 cut(s) 448, 456
Sau96I GGNCC 1 cut(s) 17
SduI GDGCHC 1 cut(s) 317
SetI ASST 5 cut(s) 37, 273, 448, 471, 492
SfcI CTRYAG 3 cut(s) 36, 76, 518
SinI GGWCC 1 cut(s) 17
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
Sse9I AATT 2 cut(s) 228, 507
SseBI AGGCCT 1 cut(s) 29
StuI AGGCCT 1 cut(s) 29
StyI CCWWGG 3 cut(s) 58, 382, 419
TaaI ACNGT 1 cut(s) 403
TaqI TCGA 1 cut(s) 343
TasI AATT 2 cut(s) 228, 507
TfiI GAWTC 2 cut(s) 8, 158
Tru1I TTAA 1 cut(s) 51
Tru9I TTAA 1 cut(s) 51
TscAI CASTG 2 cut(s) 49, 252
TseFI GTSAC 3 cut(s) 126, 253, 357
TseI GCWGC 4 cut(s) 78, 107, 239, 329
Tsp45I GTSAC 3 cut(s) 126, 253, 357
TspDTI ATGAA 2 cut(s) 17, 484
TspRI CASTG 2 cut(s) 49, 252
VpaK11BI GGWCC 1 cut(s) 17
VspI ATTAAT 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.