RchiOBHm_Chr6g0267141

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001733
Physical Location & Seq
Reverse (-)
23681891 .. 23688695
6805 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23961

Sequence Viewer

Length: 1632 bp
ATGCAAGCTAGAACCCTCCACCTCATTTCTCACTCTCTCTCTTCTCAACCTTGTTGTCTCTTCTCCACTACTCCTCCACCTTCTTCCTCTCTCTTCTTCAACCACACACCTCGATTTCCGAGCTCACGCTCACGCTGCTTCTCCTCCTCCTCAGCTTCAATACGACGCCGTCCGATTCGCCCTGTAATCGCCTGCATTGGTGATGGCGGGGCCGGAACGGACGCCATTTCGAGGTCTGGAGAAGCTGCGGAGGTTAAAGGAGGAGAGTTGGAGAGTCAGAGTATATGGAATCAGATGAAGGAGATTGTCATGTTTACTGGGCCGGCTACCGGGATCTGGATCAGCGGCCCACTTATGAGTCTCATCGACACCGTCGTCGTCGGTCAGGGAAGCTCCATTGAGCTTGCCGCATTAGGTCCTGGAACTGTAATGTGCGATAATATGAGCTATGTTTTCATGTTCCTTTCTATTGCAACTTCCAATATCGTCGCTACTTCCCTTGCAAAAGGGGATAAAAATGAAGTGCAACATCATATATCCACCTTGCTCTTTGTTGCATTGACCTGTGGCTTCTTAATGCTTTTATTTACAAGATTCTTCGGCTGTTGGGCACTGACTGCTTTTACTGGATCAAAGAACATACATATTATTCCAGCAGCAAATACATACGTTCAGATACGGAGTTTGGCATGGCCTGCAGTTCTTGTTGGTTGGGTTACCCAGAGTGCAAGTCTTGGCATGAAAGATTCATGGGGTCCTTTGAAGGCCTTAGCTGTAGCCAGTGCTATTAATGGCGTTGGTGATATACTACTCTGCAGCGTTTTAGGCTATGGTATTGCTGGTGCAGCATGGGCGACAATGCTATCACAGATTGTTGCAGGATACATGATGATTGAAACTTTGAACAAGAAAGGATACAACTCTTATGCTATTTCTGTTCCCTCACCCAAAGAACTTCTAACACTAATTGGGCTTGCTGCTCCAGTGCTGGTGACAATGATGTCCAAGGTTGCTTTCTACTCGCTCATTGTATATTTTGCAACATCTATGGGCACATATACAATGGCTGCTCATCAGGTCATGATTCAAACATTCATGATCTGTACGGTGTGGGGCGAACCTCTCTCTCAAACTGCACGGTCATTTATGCCTCAGTTGATATATGGAGTAAATCGTAGTTTGCCCAAGGCTCGAATGTTGCTCAAGTCACTTGTCATCATTGGAGCAATCCTTGGTTTGGCTCTTGGAACTGTTGGAACATCTGTTCCATGGTTGTTTCCTAACATCTTTACACCTGATCAGAAGATCATACAGGAGATGCACAAACTACTGATACCATTTTTTTTGGCATTGGCTGTGACACCCGCCATTCTCAGCTTTGAGGGAACATTGCTGGCTGGACGAGATCTTAGATTCATTAGTCTATCAATGAGTAGCTGCTTCTCTTTAGGTGCACTTCTGCTGCTGCTTGTGAGTAGCCGAGGATGTGGTTTAGTAAGCTGCTGGTGGACACTTGTAGCATTTCAATGGGCTCGGCTTTTCCTTTCTTTACGGCGCCTTATATCACCTGATGGCATACTCTACTCTGAAGATATGAGCCGGTATAAACTGGAAGAAGTCAGAGCTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

543

Amino Acids

58.7

Weight (kDa)

9.19

Isoelectric Point (pI)

48.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 117 - 265 5.3e-13 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 374, 1000
AccB1I GGYRCC 1 cut(s) 1554
AciI CCGC 5 cut(s) 207, 248, 345, 408, 1365
AclWI GGATC 3 cut(s) 341, 347, 637
AcuI CTGAAG 1 cut(s) 1608
AcyI GRCGYC 3 cut(s) 166, 222, 1555
AfaI GTAC 1 cut(s) 1105
AfiI CCNNNNNNNGG 4 cut(s) 231, 329, 336, 1237
AgsI TTSAA 7 cut(s) 100, 159, 763, 896, 904, 1088, 1526
AjnI CCWGG 1 cut(s) 418
AjuI GAANNNNNNNTTGG 2 cut(s) 998, 1030
AloI GAACNNNNNNTCC 4 cut(s) 1248, 1249, 1280, 1281
Alw21I GWGCWC 2 cut(s) 125, 1456
Alw26I GTCTC 2 cut(s) 62, 365
Alw44I GTGCAC 1 cut(s) 1452
AlwI GGATC 3 cut(s) 341, 347, 637
AoxI GGCC 5 cut(s) 210, 320, 346, 692, 765
ApaLI GTGCAC 1 cut(s) 1452
AseI ATTAAT 1 cut(s) 789
AspLEI GCGC 1 cut(s) 1557
AspS9I GGNCC 5 cut(s) 210, 320, 347, 416, 755
AsuC2I CCSGG 1 cut(s) 331
AsuHPI GGTGA 5 cut(s) 212, 812, 936, 1003, 1557
AvaII GGWCC 2 cut(s) 416, 755
BaeGI GKGCMC 3 cut(s) 613, 1055, 1456
BanI GGYRCC 1 cut(s) 1554
BanII GRGCYC 2 cut(s) 125, 1534
Bbv12I GWGCWC 2 cut(s) 125, 1456
BbvCI CCTCAGC 1 cut(s) 151
BccI CCATC 2 cut(s) 197, 1565
BceAI ACGGC 2 cut(s) 153, 1568
BciT130I CCWGG 1 cut(s) 420
BciVI GTATCC 2 cut(s) 875, 908
BclI TGATCA 1 cut(s) 1297
BcnI CCSGG 1 cut(s) 331
BcoDI GTCTC 2 cut(s) 62, 365
BfaI CTAG 1 cut(s) 9
BfmI CTRYAG 3 cut(s) 696, 774, 814
BfoI RGCGCY 1 cut(s) 1558
BfuI GTATCC 2 cut(s) 875, 908
BglII AGATCT 1 cut(s) 1405
Bme1390I CCNGG 2 cut(s) 331, 420
Bme18I GGWCC 2 cut(s) 416, 755
BmgT120I GGNCC 5 cut(s) 210, 320, 347, 416, 755
BmiI GGNNCC 3 cut(s) 211, 756, 1556
BmrFI CCNGG 2 cut(s) 331, 420
BmrI ACTGGG 1 cut(s) 327
BmsI GCATC 1 cut(s) 1308
BmuI ACTGGG 1 cut(s) 327
BpmI CTGGAG 2 cut(s) 258, 966
Bpu10I CCTNAGC 2 cut(s) 151, 769
BpuEI CTTGAG 1 cut(s) 1187
BpuMI CCSGG 1 cut(s) 331
BsaBI GATNNNNATC 1 cut(s) 338
BsaHI GRCGYC 3 cut(s) 166, 222, 1555
BsaJI CCNNGG 5 cut(s) 1005, 1185, 1231, 1268, 1480
BsaXI ACNNNNNCTCC 2 cut(s) 58, 88
Bsc4I CCNNNNNNNGG 4 cut(s) 231, 329, 336, 1237
Bse118I RCCGGY 2 cut(s) 322, 1599
Bse1I ACTGG 5 cut(s) 322, 631, 780, 983, 1614
Bse3DI GCAATG 1 cut(s) 1388
Bse8I GATNNNNATC 1 cut(s) 338
BseBI CCWGG 1 cut(s) 420
BseDI CCNNGG 5 cut(s) 1005, 1185, 1231, 1268, 1480
BseGI GGATG 1 cut(s) 1490
BseJI GATNNNNATC 1 cut(s) 338
BseLI CCNNNNNNNGG 4 cut(s) 231, 329, 336, 1237
BseMI GCAATG 1 cut(s) 1388
BseMII CTCAG 3 cut(s) 165, 1166, 1387
BseNI ACTGG 5 cut(s) 322, 631, 780, 983, 1614
BseRI GAGGAG 5 cut(s) 63, 133, 136, 139, 276
BseSI GKGCMC 3 cut(s) 613, 1055, 1456
BsgI GTGCAG 2 cut(s) 864, 1119
BshFI GGCC 5 cut(s) 212, 322, 348, 694, 767
BshNI GGYRCC 1 cut(s) 1554
BsiHKAI GWGCWC 2 cut(s) 125, 1456
BsiSI CCGG 4 cut(s) 213, 323, 330, 1600
BslI CCNNNNNNNGG 4 cut(s) 231, 329, 336, 1237
BsmAI GTCTC 2 cut(s) 62, 365
BsnI GGCC 5 cut(s) 212, 322, 348, 694, 767
Bsp1286I GDGCHC 5 cut(s) 125, 613, 1055, 1456, 1534
Bsp143I GATC 7 cut(s) 333, 339, 629, 1098, 1297, 1305, 1405
Bsp19I CCATGG 1 cut(s) 1268
BspACI CCGC 5 cut(s) 207, 248, 345, 408, 1365
BspANI GGCC 5 cut(s) 212, 322, 348, 694, 767
BspCNI CTCAG 3 cut(s) 164, 1165, 1386
BspHI TCATGA 2 cut(s) 1080, 1095
BspLI GGNNCC 3 cut(s) 211, 756, 1556
BspMAI CTGCAG 2 cut(s) 700, 818
BspPI GGATC 3 cut(s) 341, 347, 637
BspT107I GGYRCC 1 cut(s) 1554
BsrDI GCAATG 1 cut(s) 1388
BsrFI RCCGGY 2 cut(s) 322, 1599
BsrI ACTGG 5 cut(s) 322, 631, 780, 983, 1614
BssAI RCCGGY 2 cut(s) 322, 1599
BssECI CCNNGG 5 cut(s) 1005, 1185, 1231, 1268, 1480
BssMI GATC 7 cut(s) 333, 339, 629, 1098, 1297, 1305, 1405
BssNI GRCGYC 3 cut(s) 166, 222, 1555
BssT1I CCWWGG 4 cut(s) 1005, 1185, 1231, 1268
Bst2UI CCWGG 1 cut(s) 420
Bst4CI ACNGT 5 cut(s) 373, 427, 1108, 1140, 1252
Bst6I CTCTTC 3 cut(s) 46, 65, 98
BstACI GRCGYC 3 cut(s) 166, 222, 1555
BstAPI GCANNNNNTGC 2 cut(s) 617, 695
BstC8I GCNNGC 7 cut(s) 6, 193, 324, 405, 696, 975, 1395
BstDEI CTNAG 5 cut(s) 151, 769, 1152, 1373, 1409
BstDSI CCRYGG 1 cut(s) 1268
BstEII GGTNACC 1 cut(s) 715
BstF5I GGATG 1 cut(s) 1490
BstH2I RGCGCY 1 cut(s) 1558
BstHHI GCGC 1 cut(s) 1557
BstKTI GATC 7 cut(s) 336, 342, 632, 1101, 1300, 1308, 1408
BstMAI GTCTC 2 cut(s) 62, 365
BstMBI GATC 7 cut(s) 333, 339, 629, 1098, 1297, 1305, 1405
BstMWI GCNNNNNNNGC 6 cut(s) 135, 617, 695, 825, 845, 851
BstNI CCWGG 1 cut(s) 420
BstPI GGTNACC 1 cut(s) 715
BstSCI CCNGG 2 cut(s) 329, 418
BstSFI CTRYAG 3 cut(s) 696, 774, 814
BstSLI GKGCMC 3 cut(s) 613, 1055, 1456
BstX2I RGATCY 2 cut(s) 333, 1405
BstYI RGATCY 2 cut(s) 333, 1405
BsuI GTATCC 2 cut(s) 875, 908
BsuRI GGCC 5 cut(s) 212, 322, 348, 694, 767
BtgI CCRYGG 1 cut(s) 1268
BtsCI GGATG 1 cut(s) 1490
BtsIMutI CAGTG 3 cut(s) 611, 787, 990
Cac8I GCNNGC 7 cut(s) 6, 193, 324, 405, 696, 975, 1395
CciI TCATGA 2 cut(s) 1080, 1095
CfoI GCGC 1 cut(s) 1557
Cfr10I RCCGGY 2 cut(s) 322, 1599
Cfr13I GGNCC 5 cut(s) 210, 320, 347, 416, 755
CseI GACGC 2 cut(s) 174, 230
Csp6I GTAC 1 cut(s) 1104
CviQI GTAC 1 cut(s) 1104
DdeI CTNAG 5 cut(s) 151, 769, 1152, 1373, 1409
DinI GGCGCC 1 cut(s) 1556
DpnI GATC 7 cut(s) 335, 341, 631, 1100, 1299, 1307, 1407
DpnII GATC 7 cut(s) 333, 339, 629, 1098, 1297, 1305, 1405
DrdI GACNNNNNNGTC 2 cut(s) 374, 1000
DseDI GACNNNNNNGTC 2 cut(s) 374, 1000
Eam1104I CTCTTC 3 cut(s) 46, 65, 98
EarI CTCTTC 3 cut(s) 46, 65, 98
Ecl136II GAGCTC 1 cut(s) 123
Eco130I CCWWGG 4 cut(s) 1005, 1185, 1231, 1268
Eco147I AGGCCT 1 cut(s) 767
Eco24I GRGCYC 2 cut(s) 125, 1534
Eco47I GGWCC 2 cut(s) 416, 755
Eco53kI GAGCTC 1 cut(s) 123
Eco57I CTGAAG 1 cut(s) 1608
Eco91I GGTNACC 1 cut(s) 715
EcoICRI GAGCTC 1 cut(s) 123
EcoO109I RGGNCCY 2 cut(s) 416, 755
EcoO65I GGTNACC 1 cut(s) 715
EcoRII CCWGG 1 cut(s) 418
EcoT14I CCWWGG 4 cut(s) 1005, 1185, 1231, 1268
EcoT38I GRGCYC 2 cut(s) 125, 1534
EgeI GGCGCC 1 cut(s) 1556
EheI GGCGCC 1 cut(s) 1556
ErhI CCWWGG 4 cut(s) 1005, 1185, 1231, 1268
FauI CCCGC 2 cut(s) 200, 1372
FbaI TGATCA 1 cut(s) 1297
FokI GGATG 1 cut(s) 1497
FriOI GRGCYC 2 cut(s) 125, 1534
FspBI CTAG 1 cut(s) 9
GlaI GCGC 1 cut(s) 1556
GsuI CTGGAG 2 cut(s) 258, 966
HaeII RGCGCY 1 cut(s) 1558
HaeIII GGCC 5 cut(s) 212, 322, 348, 694, 767
HapII CCGG 4 cut(s) 213, 323, 330, 1600
HgaI GACGC 2 cut(s) 174, 230
HhaI GCGC 1 cut(s) 1557
Hin1I GRCGYC 3 cut(s) 166, 222, 1555
Hin6I GCGC 1 cut(s) 1555
HinP1I GCGC 1 cut(s) 1555
HinfI GANTC 8 cut(s) 175, 274, 289, 358, 594, 746, 1084, 1413
HpaII CCGG 4 cut(s) 213, 323, 330, 1600
HphI GGTGA 5 cut(s) 212, 812, 936, 1003, 1557
Hpy166II GTNNAC 3 cut(s) 315, 1454, 1509
Hpy188I TCNGA 8 cut(s) 120, 174, 279, 294, 675, 1302, 1588, 1622
Hpy188III TCNNGA 4 cut(s) 237, 337, 1081, 1096
Hpy8I GTNNAC 3 cut(s) 315, 1454, 1509
Hpy99I CGWCG 5 cut(s) 168, 377, 380, 383, 491
HpyAV CCTTC 3 cut(s) 90, 292, 757
HpyCH4III ACNGT 5 cut(s) 373, 427, 1108, 1140, 1252
HpyCH4IV ACGT 1 cut(s) 669
HpyF10VI GCNNNNNNNGC 6 cut(s) 135, 617, 695, 825, 845, 851
HpyF3I CTNAG 5 cut(s) 151, 769, 1152, 1373, 1409
HpySE526I ACGT 1 cut(s) 669
Hsp92I GRCGYC 3 cut(s) 166, 222, 1555
HspAI GCGC 1 cut(s) 1555
KasI GGCGCC 1 cut(s) 1554
KroI GCCGGC 1 cut(s) 322
KroNI GCCGGC 1 cut(s) 324
Ksp22I TGATCA 1 cut(s) 1297
Kzo9I GATC 7 cut(s) 333, 339, 629, 1098, 1297, 1305, 1405
LmnI GCTCC 3 cut(s) 398, 985, 1223
LweI GCATC 1 cut(s) 1308
MaeI CTAG 1 cut(s) 9
MaeII ACGT 1 cut(s) 669
MaeIII GTNAC 4 cut(s) 715, 991, 1206, 1357
MalI GATC 7 cut(s) 335, 341, 631, 1100, 1299, 1307, 1407
MboI GATC 7 cut(s) 333, 339, 629, 1098, 1297, 1305, 1405
MboII GAAGA 9 cut(s) 33, 52, 75, 85, 88, 589, 1315, 1601, 1625
MflI RGATCY 2 cut(s) 333, 1405
MhlI GDGCHC 5 cut(s) 125, 613, 1055, 1456, 1534
MluCI AATT 1 cut(s) 966
Mly113I GGCGCC 1 cut(s) 1555
MlyI GAGTC 2 cut(s) 283, 367
MmeI TCCRAC 2 cut(s) 249, 1234
MroNI GCCGGC 1 cut(s) 322
MseI TTAA 3 cut(s) 255, 575, 789
MslI CAYNNNNRTG 1 cut(s) 1525
MspA1I CMGCKG 1 cut(s) 345
MspI CCGG 4 cut(s) 213, 323, 330, 1600
MspR9I CCNGG 2 cut(s) 331, 420
MvaI CCWGG 1 cut(s) 420
MwoI GCNNNNNNNGC 6 cut(s) 135, 617, 695, 825, 845, 851
NaeI GCCGGC 1 cut(s) 324
NarI GGCGCC 1 cut(s) 1555
NciI CCSGG 1 cut(s) 331
NcoI CCATGG 1 cut(s) 1268
NdeII GATC 7 cut(s) 333, 339, 629, 1098, 1297, 1305, 1405
NgoMIV GCCGGC 1 cut(s) 322
NlaIV GGNNCC 3 cut(s) 211, 756, 1556
NmeAIII GCCGAG 2 cut(s) 1505, 1513
NmuCI GTSAC 3 cut(s) 991, 1206, 1357
PagI TCATGA 2 cut(s) 1080, 1095
PceI AGGCCT 1 cut(s) 767
PcsI WCGNNNNNNNCGW 1 cut(s) 372
PdiI GCCGGC 1 cut(s) 324
PfeI GAWTC 6 cut(s) 175, 289, 594, 746, 1084, 1413
PflFI GACNNNGTC 2 cut(s) 168, 371
PfoI TCCNGGA 1 cut(s) 418
PleI GAGTC 2 cut(s) 282, 366
PluTI GGCGCC 1 cut(s) 1558
PpsI GAGTC 2 cut(s) 282, 366
PpuMI RGGWCCY 2 cut(s) 416, 755
PshBI ATTAAT 1 cut(s) 789
Psp124BI GAGCTC 1 cut(s) 125
Psp5II RGGWCCY 2 cut(s) 416, 755
Psp6I CCWGG 1 cut(s) 418
PspEI GGTNACC 1 cut(s) 715
PspGI CCWGG 1 cut(s) 418
PspN4I GGNNCC 3 cut(s) 211, 756, 1556
PspPI GGNCC 5 cut(s) 210, 320, 347, 416, 755
PspPPI RGGWCCY 2 cut(s) 416, 755
PstI CTGCAG 2 cut(s) 700, 818
PsuI RGATCY 2 cut(s) 333, 1405
PsyI GACNNNGTC 2 cut(s) 168, 371
RsaI GTAC 1 cut(s) 1105
RsaNI GTAC 1 cut(s) 1104
RseI CAYNNNNRTG 1 cut(s) 1525
SacI GAGCTC 1 cut(s) 125
SaqAI TTAA 3 cut(s) 255, 575, 789
Sau3AI GATC 7 cut(s) 333, 339, 629, 1098, 1297, 1305, 1405
Sau96I GGNCC 5 cut(s) 210, 320, 347, 416, 755
SchI GAGTC 2 cut(s) 283, 367
ScrFI CCNGG 2 cut(s) 331, 420
SduI GDGCHC 5 cut(s) 125, 613, 1055, 1456, 1534
SfaNI GCATC 1 cut(s) 1308
SfcI CTRYAG 3 cut(s) 696, 774, 814
SfoI GGCGCC 1 cut(s) 1556
SinI GGWCC 2 cut(s) 416, 755
SmiMI CAYNNNNRTG 1 cut(s) 1525
SmlI CTYRAG 1 cut(s) 1202
SmoI CTYRAG 1 cut(s) 1202
Sse9I AATT 1 cut(s) 966
SseBI AGGCCT 1 cut(s) 767
SsiI CCGC 5 cut(s) 207, 248, 345, 408, 1365
SspDI GGCGCC 1 cut(s) 1554
SspMI CTAG 1 cut(s) 9
SstI GAGCTC 1 cut(s) 125
StuI AGGCCT 1 cut(s) 767
StyD4I CCNGG 2 cut(s) 329, 418
StyI CCWWGG 4 cut(s) 1005, 1185, 1231, 1268
TaaI ACNGT 5 cut(s) 373, 427, 1108, 1140, 1252
TaiI ACGT 1 cut(s) 672
TaqI TCGA 4 cut(s) 112, 230, 366, 1192
TaqII GACCGA 1 cut(s) 371
TasI AATT 1 cut(s) 966
TauI GCSGC 2 cut(s) 348, 410
TfiI GAWTC 6 cut(s) 175, 289, 594, 746, 1084, 1413
Tru1I TTAA 3 cut(s) 255, 575, 789
Tru9I TTAA 3 cut(s) 255, 575, 789
TscAI CASTG 3 cut(s) 618, 787, 990
TseFI GTSAC 3 cut(s) 991, 1206, 1357
Tsp45I GTSAC 3 cut(s) 991, 1206, 1357
TspDTI ATGAA 7 cut(s) 311, 445, 534, 738, 755, 1084, 1405
TspGWI ACGGA 2 cut(s) 233, 694
TspRI CASTG 3 cut(s) 618, 787, 990
Tth111I GACNNNGTC 2 cut(s) 168, 371
VneI GTGCAC 1 cut(s) 1452
VpaK11BI GGWCC 2 cut(s) 416, 755
VspI ATTAAT 1 cut(s) 789
XspI CTAG 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.