Rorug06G0029100

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001733
Physical Location & Seq
Reverse (-)
3456704 .. 3457813
1110 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0029100.1

Sequence Viewer

Length: 426 bp
ATGGGTATCAAATTGCTATCAACTATTCGGCGATCTCTACTGACGTCTAAGCTTTCAAGTGTGGCTACTACGAATGTCCCAAAGGGCCACTTGGCTGTTTATGTAGGGGAAGAACAGAAAAAGAGATTCATCATACCAATATCATACTTGAACCACCAGTGGTTCAAAGAGTTGCTAAATTTTTCTGAAGAGAAATTTGGATTCACTCATCCAATGGGTGGTATCACTATCCCATGCACTGAGGAGGCCTTCATTGGTTTCAGTCGTTCTTGTTTACATTCCTCTCTGTCTAACCATGACAGTCGACTCCTAGTGATAGGCTGTCCATGTGAATTAGTCAAGAGGGGACCCAATTATAATGGTTTAGCTAGCCTAAAAGGTAATCTTAAAGTAGAAATGATCATATGGATGAATTCTGGGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

141

Amino Acids

15.73

Weight (kDa)

9.33

Isoelectric Point (pI)

38.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 18 - 84 6.6e-23 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 357
AatII GACGTC 1 cut(s) 47
AccB7I CCANNNNNTGG 1 cut(s) 218
AccI GTMKAC 1 cut(s) 304
AcsI RAATTY 3 cut(s) 178, 194, 412
AcuI CTGAAG 1 cut(s) 207
AcyI GRCGYC 1 cut(s) 44
AfiI CCNNNNNNNGG 1 cut(s) 218
AgsI TTSAA 3 cut(s) 57, 151, 166
AjuI GAANNNNNNNTTGG 2 cut(s) 180, 212
AluBI AGCT 2 cut(s) 52, 368
AluI AGCT 2 cut(s) 52, 368
AoxI GGCC 2 cut(s) 85, 246
ApoI RAATTY 3 cut(s) 178, 194, 412
AspS9I GGNCC 2 cut(s) 85, 347
AsuNHI GCTAGC 1 cut(s) 368
AvaII GGWCC 1 cut(s) 347
BclI TGATCA 1 cut(s) 399
BfaI CTAG 2 cut(s) 311, 369
Bme18I GGWCC 1 cut(s) 347
BmgT120I GGNCC 2 cut(s) 85, 347
BmiI GGNNCC 2 cut(s) 348, 349
BmtI GCTAGC 1 cut(s) 372
BsaHI GRCGYC 1 cut(s) 44
Bsc4I CCNNNNNNNGG 1 cut(s) 218
Bse1I ACTGG 1 cut(s) 157
BseGI GGATG 2 cut(s) 208, 414
BseLI CCNNNNNNNGG 1 cut(s) 218
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 1 cut(s) 157
BseRI GAGGAG 1 cut(s) 257
BshFI GGCC 2 cut(s) 87, 248
BslFI GGGAC 2 cut(s) 62, 360
BslI CCNNNNNNNGG 1 cut(s) 218
BsmFI GGGAC 2 cut(s) 62, 360
BsnI GGCC 2 cut(s) 87, 248
Bsp143I GATC 2 cut(s) 32, 399
BspANI GGCC 2 cut(s) 87, 248
BspCNI CTCAG 1 cut(s) 232
BspLI GGNNCC 2 cut(s) 348, 349
BspOI GCTAGC 1 cut(s) 372
BsrI ACTGG 1 cut(s) 157
BssMI GATC 2 cut(s) 32, 399
BssNI GRCGYC 1 cut(s) 44
Bst4CI ACNGT 1 cut(s) 302
Bst6I CTCTTC 1 cut(s) 183
BstACI GRCGYC 1 cut(s) 44
BstC8I GCNNGC 1 cut(s) 370
BstDEI CTNAG 2 cut(s) 48, 240
BstF5I GGATG 2 cut(s) 208, 414
BstKTI GATC 2 cut(s) 35, 402
BstMBI GATC 2 cut(s) 32, 399
BsuRI GGCC 2 cut(s) 87, 248
BtsCI GGATG 2 cut(s) 208, 414
BtsIMutI CAGTG 2 cut(s) 164, 237
Cac8I GCNNGC 1 cut(s) 370
Cfr13I GGNCC 2 cut(s) 85, 347
CviAII CATG 3 cut(s) 234, 296, 327
CviJI RGCY 8 cut(s) 52, 65, 87, 95, 248, 321, 368, 372
CviKI_1 RGCY 8 cut(s) 52, 65, 87, 95, 248, 321, 368, 372
DdeI CTNAG 2 cut(s) 48, 240
DpnI GATC 2 cut(s) 34, 401
DpnII GATC 2 cut(s) 32, 399
Eam1104I CTCTTC 1 cut(s) 183
EarI CTCTTC 1 cut(s) 183
Eco147I AGGCCT 1 cut(s) 248
Eco47I GGWCC 1 cut(s) 347
Eco57I CTGAAG 1 cut(s) 207
EcoO109I RGGNCCY 1 cut(s) 347
EcoRI GAATTC 1 cut(s) 412
FaeI CATG 3 cut(s) 237, 299, 330
FaiI YATR 9 cut(s) 102, 134, 145, 235, 297, 328, 357, 404, 406
FalI AAGNNNNNCTT 4 cut(s) 74, 106, 369, 401
FaqI GGGAC 2 cut(s) 62, 360
FatI CATG 3 cut(s) 233, 295, 326
FauNDI CATATG 1 cut(s) 404
FbaI TGATCA 1 cut(s) 399
FblI GTMKAC 1 cut(s) 304
FokI GGATG 2 cut(s) 195, 421
FspBI CTAG 2 cut(s) 311, 369
HaeIII GGCC 2 cut(s) 87, 248
Hin1I GRCGYC 1 cut(s) 44
Hin1II CATG 3 cut(s) 237, 299, 330
HincII GTYRAC 1 cut(s) 305
HindII GTYRAC 1 cut(s) 305
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 3 cut(s) 126, 201, 306
Hpy166II GTNNAC 2 cut(s) 275, 305
Hpy188I TCNGA 1 cut(s) 187
Hpy188III TCNNGA 1 cut(s) 340
Hpy8I GTNNAC 2 cut(s) 275, 305
HpyAV CCTTC 1 cut(s) 259
HpyCH4III ACNGT 1 cut(s) 302
HpyCH4IV ACGT 1 cut(s) 44
HpyCH4V TGCA 1 cut(s) 237
HpyF3I CTNAG 2 cut(s) 48, 240
HpySE526I ACGT 1 cut(s) 44
Hsp92I GRCGYC 1 cut(s) 44
Hsp92II CATG 3 cut(s) 237, 299, 330
KflI GGGWCCC 1 cut(s) 347
Ksp22I TGATCA 1 cut(s) 399
Kzo9I GATC 2 cut(s) 32, 399
LpnPI CCDG 2 cut(s) 170, 402
MaeI CTAG 2 cut(s) 311, 369
MaeII ACGT 1 cut(s) 44
MalI GATC 2 cut(s) 34, 401
MboI GATC 2 cut(s) 32, 399
MboII GAAGA 2 cut(s) 122, 200
MluCI AATT 6 cut(s) 11, 178, 194, 332, 352, 412
MlyI GAGTC 1 cut(s) 300
MnlI CCTC 4 cut(s) 235, 238, 292, 336
MseI TTAA 1 cut(s) 387
MslI CAYNNNNRTG 1 cut(s) 407
NdeI CATATG 1 cut(s) 404
NdeII GATC 2 cut(s) 32, 399
NheI GCTAGC 1 cut(s) 368
NlaIII CATG 3 cut(s) 237, 299, 330
NlaIV GGNNCC 2 cut(s) 348, 349
PceI AGGCCT 1 cut(s) 248
PfeI GAWTC 2 cut(s) 126, 201
PflMI CCANNNNNTGG 1 cut(s) 218
PleI GAGTC 1 cut(s) 300
PpsI GAGTC 1 cut(s) 300
PpuMI RGGWCCY 1 cut(s) 347
PsiI TTATAA 1 cut(s) 357
Psp5II RGGWCCY 1 cut(s) 347
PspN4I GGNNCC 2 cut(s) 348, 349
PspPI GGNCC 2 cut(s) 85, 347
PspPPI RGGWCCY 1 cut(s) 347
RseI CAYNNNNRTG 1 cut(s) 407
SalI GTCGAC 1 cut(s) 303
SaqAI TTAA 1 cut(s) 387
Sau3AI GATC 2 cut(s) 32, 399
Sau96I GGNCC 2 cut(s) 85, 347
SchI GAGTC 1 cut(s) 300
SetI ASST 4 cut(s) 47, 54, 370, 382
SinI GGWCC 1 cut(s) 347
SmiMI CAYNNNNRTG 1 cut(s) 407
Sse9I AATT 6 cut(s) 11, 178, 194, 332, 352, 412
SseBI AGGCCT 1 cut(s) 248
SspMI CTAG 2 cut(s) 311, 369
StuI AGGCCT 1 cut(s) 248
TaaI ACNGT 1 cut(s) 302
TaiI ACGT 1 cut(s) 47
TaqI TCGA 1 cut(s) 304
TasI AATT 6 cut(s) 11, 178, 194, 332, 352, 412
TfiI GAWTC 2 cut(s) 126, 201
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TscAI CASTG 2 cut(s) 164, 244
TspDTI ATGAA 3 cut(s) 118, 241, 425
TspRI CASTG 2 cut(s) 164, 244
Van91I CCANNNNNTGG 1 cut(s) 218
VpaK11BI GGWCC 1 cut(s) 347
XapI RAATTY 3 cut(s) 178, 194, 412
XmiI GTMKAC 1 cut(s) 304
XspI CTAG 2 cut(s) 311, 369
ZraI GACGTC 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.