pycom10g05090

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001733
Physical Location & Seq
Forward (+)
5411126 .. 5417696
6571 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g05090.10

Sequence Viewer

Length: 1674 bp
ATGAAAATGCAAACCAAAACCCTGATAGCTCACCCTCCTCACTCTCCCCTCCTCCAAAACCCTACTCCCAACGCTCTTTCTCAACCTTCTCTCTCCTTCACCAGTCCATCTCTCTCTTTCCCCCCACGCTTTCACCACACACCTGCACTTTGCCTCCCATGGCTACGCTCATCCGCGCCTATACGGCGTCGTAATCGGTTCTCAGCCGCCTGCAATGCCCAAACACCTGGAGTCCGCGGCTCAGACGATGGAGGGGACGATGATGCTTGGGTGTCTGCGGAGGGAGTTGTGGAGGTTAAGAAAGAAGTGCTAGAGAGTCAGAATCTGTGGAGTCGGATGAAGGAGATTGCAGTGTTTACAGGACCCGCCACTGGGCTTTGGCTGTGTGGCCCACTTATGAGTCTCATCGACACCGTCGTCATCGGTCAGGGAAGCTCCATTGAGCTTGCTGCCTTGGGCCCTGGAACTGTGATGTGTGATTATATGAGTTATGTGTTCATGTTCCTTTCAATTGCAACTTCAAACATGGTTGCCACTGCGCTTGCTAGGCGGGATAAAAGTGAAGTGCAACATCAGATATCTATTTTGCTCTTTGTTGGATTGGCCAGTGGCTTCTTAATGCTTTTATTTACAAGATTCTTTGGTTCATGGGCACTAACTGCTTTTACTGGATCAAAGAATGTGGATATTATTCATGCAGCAAATACATATGTTCAGATACGAGGATTGGCATGGCCTGCACTTCTTGTTGGTTGGGTTGCTCAGAGTGCAAGTCTTGGTATGAAAGATTCCTGGGGTCCTTTGAAGGCATTGGCTGTAGCCAGTGCCGTAAATGGAGTTGGTGATGTACTCTTGTGCAGCTTTTTAGGCTGTGGTATTGCTGGTGCAGCGTGGGCAACAATGGTGTCACAGGTTGTTGCAGGATATATGATGACTGAAGCTCTCAACAAGAAAGGATACAATGCCTATGACATCTCTGTTCCCTCATCCAAGGAACTTCTAACAGTACTTGGACTTGCTGCTCCCGTATTTATTACAATGATGTCAAAGGTGGCTTTCTACTCTCTCCTTGTGTATTTTGCGACATCTATGGGAACAAATACCATGGCTGCTCATCAGGTCATGATTCAAACATTCTGCATGTGTACGGTGTGGGGTGAACCTCTCTCTCAAACTGCACAATCGTTTATGCCCGAGTTTATATATGGAGTAAATCGAAGTTTGGCAAAGGCTCGAATGCTGCTCAAGTCACTTGTCATCATTGGAGCTATACTTGGTTCAGTATTAGGGATTGTCGGAACATATATTCCTTGGTTGTTTCCCAATATCTTTACACCTGATCAGATGATCATTCAGGAGATGCATAAAGTGCTGGTACCATATTTTCTTGCATTAATTGTGACGCCCCCCACTCACAGCCTTGAGGGAACATTGCTGGCCGGACGGGATCTCAGATTTATTAGTCTGTCCATGAGCGGATGCTTTTCCTTAGGTGCACTGCTATTGCTGCTTGTGAGCAGTAGAGGAGTTGGCTTAGCAGGCTGCTGGTGGGCACTGGCAGCATTCCAGTGGAGTCGGTTTTTCCTCTCTCTACAACGGCTGATTTCTCCCAACGGCATACTTTATTCTGAAGATATGACCCGCTTCGAATTGGAAAAACTCAAGGCTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

558

Amino Acids

60.39

Weight (kDa)

8.84

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 151
AasI GACNNNNNNGTC 1 cut(s) 416
Acc36I ACCTGC 1 cut(s) 151
Acc65I GGTACC 1 cut(s) 1375
AccB1I GGYRCC 1 cut(s) 1375
AccBSI CCGCTC 1 cut(s) 1476
AccII CGCG 2 cut(s) 176, 237
AciI CCGC 9 cut(s) 174, 207, 235, 237, 278, 366, 550, 1476, 1642
AclWI GGATC 2 cut(s) 679, 1455
AcoI YGGCCR 2 cut(s) 603, 1437
AcuI CTGAAG 2 cut(s) 957, 1650
AcyI GRCGYC 2 cut(s) 187, 1403
AfaI GTAC 4 cut(s) 849, 1008, 1147, 1377
AfiI CCNNNNNNNGG 2 cut(s) 371, 372
AgsI TTSAA 4 cut(s) 510, 522, 805, 1130
AjnI CCWGG 3 cut(s) 226, 460, 791
AloI GAACNNNNNNTCC 2 cut(s) 1291, 1323
AluBI AGCT 6 cut(s) 29, 435, 445, 861, 941, 1268
AluI AGCT 6 cut(s) 29, 435, 445, 861, 941, 1268
Alw21I GWGCWC 1 cut(s) 1498
Alw26I GTCTC 1 cut(s) 407
Alw44I GTGCAC 1 cut(s) 1494
AlwI GGATC 2 cut(s) 679, 1455
AlwNI CAGNNNCTG 1 cut(s) 325
Ama87I CYCGRG 1 cut(s) 1193
AoxI GGCC 5 cut(s) 388, 457, 603, 734, 1437
ApaI GGGCCC 1 cut(s) 461
ApaLI GTGCAC 1 cut(s) 1494
AseI ATTAAT 1 cut(s) 1394
Asp718I GGTACC 1 cut(s) 1375
AspLEI GCGC 2 cut(s) 178, 541
AspS9I GGNCC 5 cut(s) 362, 389, 457, 458, 797
AsuHPI GGTGA 5 cut(s) 23, 91, 125, 854, 1169
AsuII TTCGAA 1 cut(s) 1647
AvaI CYCGRG 1 cut(s) 1193
AvaII GGWCC 2 cut(s) 362, 797
AxyI CCTNAGG 1 cut(s) 1489
BaeGI GKGCMC 4 cut(s) 461, 655, 1498, 1555
BalI TGGCCA 1 cut(s) 605
BanI GGYRCC 1 cut(s) 1375
BanII GRGCYC 1 cut(s) 461
Bbv12I GWGCWC 1 cut(s) 1498
BccI CCATC 2 cut(s) 115, 242
BceAI ACGGC 4 cut(s) 200, 812, 1613, 1630
BciT130I CCWGG 3 cut(s) 228, 462, 793
BciVI GTATCC 1 cut(s) 950
BclI TGATCA 2 cut(s) 1339, 1347
BcoDI GTCTC 1 cut(s) 407
BfaI CTAG 2 cut(s) 311, 546
BfmI CTRYAG 1 cut(s) 816
BfuAI ACCTGC 1 cut(s) 151
BfuI GTATCC 1 cut(s) 950
BglI GCCNNNNNGGC 1 cut(s) 184
BlpI GCTNAGC 1 cut(s) 1534
BmcAI AGTACT 1 cut(s) 1008
Bme1390I CCNGG 3 cut(s) 228, 462, 793
Bme18I GGWCC 2 cut(s) 362, 797
BmeT110I CYCGRG 1 cut(s) 1193
BmgT120I GGNCC 5 cut(s) 362, 389, 457, 458, 797
BmiI GGNNCC 4 cut(s) 364, 459, 798, 1377
BmrFI CCNGG 3 cut(s) 228, 462, 793
BmrI ACTGGG 1 cut(s) 381
BmsI GCATC 3 cut(s) 253, 1350, 1469
BmuI ACTGGG 1 cut(s) 381
BpmI CTGGAG 1 cut(s) 249
Bpu1102I GCTNAGC 1 cut(s) 1534
Bpu14I TTCGAA 1 cut(s) 1647
BpuEI CTTGAG 3 cut(s) 1229, 1442, 1646
BsaHI GRCGYC 2 cut(s) 187, 1403
BsaJI CCNNGG 8 cut(s) 158, 235, 453, 460, 792, 990, 1104, 1310
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bsc4I CCNNNNNNNGG 2 cut(s) 371, 372
Bse1I ACTGG 7 cut(s) 102, 376, 606, 673, 822, 1560, 1567
Bse21I CCTNAGG 1 cut(s) 1489
Bse3DI GCAATG 2 cut(s) 220, 1430
BseBI CCWGG 3 cut(s) 228, 462, 793
BseDI CCNNGG 8 cut(s) 158, 235, 453, 460, 792, 990, 1104, 1310
BseGI GGATG 4 cut(s) 170, 342, 986, 1484
BseLI CCNNNNNNNGG 2 cut(s) 371, 372
BseMI GCAATG 2 cut(s) 220, 1430
BseMII CTCAG 4 cut(s) 216, 255, 776, 1465
BseNI ACTGG 7 cut(s) 102, 376, 606, 673, 822, 1560, 1567
BseRI GAGGAG 3 cut(s) 27, 41, 1539
BseSI GKGCMC 4 cut(s) 461, 655, 1498, 1555
BsgI GTGCAG 5 cut(s) 129, 723, 877, 906, 1161
Bsh1236I CGCG 2 cut(s) 176, 237
BshFI GGCC 5 cut(s) 390, 459, 605, 736, 1439
BshNI GGYRCC 1 cut(s) 1375
BsiHKAI GWGCWC 1 cut(s) 1498
BsiHKCI CYCGRG 1 cut(s) 1193
BsiSI CCGG 1 cut(s) 1440
BslFI GGGAC 1 cut(s) 269
BslI CCNNNNNNNGG 2 cut(s) 371, 372
BsmAI GTCTC 1 cut(s) 407
BsmFI GGGAC 1 cut(s) 269
BsmI GAATGC 2 cut(s) 1242, 1562
BsnI GGCC 5 cut(s) 390, 459, 605, 736, 1439
BsoBI CYCGRG 1 cut(s) 1193
Bsp119I TTCGAA 1 cut(s) 1647
Bsp120I GGGCCC 1 cut(s) 457
Bsp1286I GDGCHC 4 cut(s) 461, 655, 1498, 1555
Bsp143I GATC 4 cut(s) 671, 1339, 1347, 1447
Bsp1720I GCTNAGC 1 cut(s) 1534
Bsp19I CCATGG 2 cut(s) 158, 1104
BspACI CCGC 9 cut(s) 174, 207, 235, 237, 278, 366, 550, 1476, 1642
BspANI GGCC 5 cut(s) 390, 459, 605, 736, 1439
BspCNI CTCAG 4 cut(s) 215, 254, 775, 1464
BspFNI CGCG 2 cut(s) 176, 237
BspHI TCATGA 1 cut(s) 1122
BspLI GGNNCC 4 cut(s) 364, 459, 798, 1377
BspMI ACCTGC 1 cut(s) 151
BspPI GGATC 2 cut(s) 679, 1455
BspT104I TTCGAA 1 cut(s) 1647
BspT107I GGYRCC 1 cut(s) 1375
BsrBI CCGCTC 1 cut(s) 1476
BsrDI GCAATG 2 cut(s) 220, 1430
BsrI ACTGG 7 cut(s) 102, 376, 606, 673, 822, 1560, 1567
BssECI CCNNGG 8 cut(s) 158, 235, 453, 460, 792, 990, 1104, 1310
BssMI GATC 4 cut(s) 671, 1339, 1347, 1447
BssNI GRCGYC 2 cut(s) 187, 1403
BssT1I CCWWGG 5 cut(s) 158, 453, 990, 1104, 1310
Bst2UI CCWGG 3 cut(s) 228, 462, 793
Bst4CI ACNGT 4 cut(s) 415, 469, 1006, 1150
BstACI GRCGYC 2 cut(s) 187, 1403
BstAPI GCANNNNNTGC 3 cut(s) 659, 737, 1369
BstBI TTCGAA 1 cut(s) 1647
BstC8I GCNNGC 6 cut(s) 211, 447, 543, 738, 1437, 1540
BstDEI CTNAG 6 cut(s) 202, 241, 762, 1451, 1489, 1534
BstDSI CCRYGG 3 cut(s) 158, 235, 1104
BstF5I GGATG 4 cut(s) 170, 342, 986, 1484
BstFNI CGCG 2 cut(s) 176, 237
BstHHI GCGC 2 cut(s) 178, 541
BstKTI GATC 4 cut(s) 674, 1342, 1350, 1450
BstMAI GTCTC 1 cut(s) 407
BstMBI GATC 4 cut(s) 671, 1339, 1347, 1447
BstNI CCWGG 3 cut(s) 228, 462, 793
BstNSI RCATGY 1 cut(s) 1144
BstSCI CCNGG 3 cut(s) 226, 460, 791
BstSFI CTRYAG 1 cut(s) 816
BstSLI GKGCMC 4 cut(s) 461, 655, 1498, 1555
BstUI CGCG 2 cut(s) 176, 237
BstX2I RGATCY 1 cut(s) 1447
BstXI CCANNNNNNTGG 1 cut(s) 227
BstYI RGATCY 1 cut(s) 1447
Bsu36I CCTNAGG 1 cut(s) 1489
BsuI GTATCC 1 cut(s) 950
BsuRI GGCC 5 cut(s) 390, 459, 605, 736, 1439
BtgI CCRYGG 3 cut(s) 158, 235, 1104
BtsCI GGATG 4 cut(s) 170, 342, 986, 1484
BtsI GCAGTG 3 cut(s) 357, 534, 1496
BtsIMutI CAGTG 8 cut(s) 357, 369, 534, 613, 829, 1496, 1553, 1574
BveI ACCTGC 1 cut(s) 151
Cac8I GCNNGC 6 cut(s) 211, 447, 543, 738, 1437, 1540
CaiI CAGNNNCTG 1 cut(s) 325
CciI TCATGA 1 cut(s) 1122
CfoI GCGC 2 cut(s) 178, 541
Cfr13I GGNCC 5 cut(s) 362, 389, 457, 458, 797
Cfr42I CCGCGG 1 cut(s) 238
CseI GACGC 2 cut(s) 176, 1411
Csp6I GTAC 4 cut(s) 848, 1007, 1146, 1376
CspCI CAANNNNNGTGG 4 cut(s) 523, 558, 663, 698
CviQI GTAC 4 cut(s) 848, 1007, 1146, 1376
DdeI CTNAG 6 cut(s) 202, 241, 762, 1451, 1489, 1534
DpnI GATC 4 cut(s) 673, 1341, 1349, 1449
DpnII GATC 4 cut(s) 671, 1339, 1347, 1447
DrdI GACNNNNNNGTC 1 cut(s) 416
DseDI GACNNNNNNGTC 1 cut(s) 416
EaeI YGGCCR 2 cut(s) 603, 1437
Eco130I CCWWGG 5 cut(s) 158, 453, 990, 1104, 1310
Eco24I GRGCYC 1 cut(s) 461
Eco32I GATATC 1 cut(s) 579
Eco47I GGWCC 2 cut(s) 362, 797
Eco57I CTGAAG 2 cut(s) 957, 1650
Eco81I CCTNAGG 1 cut(s) 1489
Eco88I CYCGRG 1 cut(s) 1193
EcoO109I RGGNCCY 3 cut(s) 362, 458, 797
EcoRII CCWGG 3 cut(s) 226, 460, 791
EcoRV GATATC 1 cut(s) 579
EcoT14I CCWWGG 5 cut(s) 158, 453, 990, 1104, 1310
EcoT22I ATGCAT 1 cut(s) 1365
EcoT38I GRGCYC 1 cut(s) 461
ErhI CCWWGG 5 cut(s) 158, 453, 990, 1104, 1310
FaqI GGGAC 1 cut(s) 269
FauI CCCGC 3 cut(s) 373, 543, 1649
FauNDI CATATG 1 cut(s) 709
FbaI TGATCA 2 cut(s) 1339, 1347
FokI GGATG 4 cut(s) 157, 349, 973, 1491
FriOI GRGCYC 1 cut(s) 461
FspBI CTAG 2 cut(s) 311, 546
GlaI GCGC 2 cut(s) 177, 540
GsuI CTGGAG 1 cut(s) 249
HaeIII GGCC 5 cut(s) 390, 459, 605, 736, 1439
HapII CCGG 1 cut(s) 1440
HgaI GACGC 2 cut(s) 176, 1411
HhaI GCGC 2 cut(s) 178, 541
Hin1I GRCGYC 2 cut(s) 187, 1403
Hin6I GCGC 2 cut(s) 176, 539
HinP1I GCGC 2 cut(s) 176, 539
HinfI GANTC 9 cut(s) 231, 316, 322, 331, 400, 636, 788, 1126, 1573
HpaII CCGG 1 cut(s) 1440
HphI GGTGA 5 cut(s) 23, 91, 125, 854, 1169
Hpy166II GTNNAC 4 cut(s) 357, 1146, 1160, 1496
Hpy188III TCNNGA 2 cut(s) 1123, 1355
Hpy8I GTNNAC 4 cut(s) 357, 1146, 1160, 1496
Hpy99I CGWCG 2 cut(s) 192, 419
HpyAV CCTTC 4 cut(s) 96, 106, 334, 799
HpyCH4III ACNGT 4 cut(s) 415, 469, 1006, 1150
HpyF3I CTNAG 6 cut(s) 202, 241, 762, 1451, 1489, 1534
Hsp92I GRCGYC 2 cut(s) 187, 1403
HspAI GCGC 2 cut(s) 176, 539
KpnI GGTACC 1 cut(s) 1379
Ksp22I TGATCA 2 cut(s) 1339, 1347
KspI CCGCGG 1 cut(s) 238
Kzo9I GATC 4 cut(s) 671, 1339, 1347, 1447
LmnI GCTCC 3 cut(s) 440, 1027, 1265
LweI GCATC 3 cut(s) 253, 1350, 1469
MaeI CTAG 2 cut(s) 311, 546
MaeIII GTNAC 3 cut(s) 906, 1248, 1399
MalI GATC 4 cut(s) 673, 1341, 1349, 1449
MbiI CCGCTC 1 cut(s) 1476
MboI GATC 4 cut(s) 671, 1339, 1347, 1447
MboII GAAGA 1 cut(s) 1643
MfeI CAATTG 1 cut(s) 510
MflI RGATCY 1 cut(s) 1447
MhlI GDGCHC 4 cut(s) 461, 655, 1498, 1555
MlsI TGGCCA 1 cut(s) 605
MluCI AATT 3 cut(s) 510, 1395, 1649
MluNI TGGCCA 1 cut(s) 605
MlyI GAGTC 5 cut(s) 240, 325, 340, 409, 1582
MmeI TCCRAC 3 cut(s) 314, 577, 1276
Mox20I TGGCCA 1 cut(s) 605
Mph1103I ATGCAT 1 cut(s) 1365
MscI TGGCCA 1 cut(s) 605
MseI TTAA 3 cut(s) 297, 617, 1394
MslI CAYNNNNRTG 1 cut(s) 1567
Msp20I TGGCCA 1 cut(s) 605
MspA1I CMGCKG 1 cut(s) 237
MspI CCGG 1 cut(s) 1440
MspR9I CCNGG 3 cut(s) 228, 462, 793
MunI CAATTG 1 cut(s) 510
Mva1269I GAATGC 2 cut(s) 1242, 1562
MvaI CCWGG 3 cut(s) 228, 462, 793
MvnI CGCG 2 cut(s) 176, 237
NcoI CCATGG 2 cut(s) 158, 1104
NdeI CATATG 1 cut(s) 709
NdeII GATC 4 cut(s) 671, 1339, 1347, 1447
NlaIV GGNNCC 4 cut(s) 364, 459, 798, 1377
NmuCI GTSAC 3 cut(s) 906, 1248, 1399
NsiI ATGCAT 1 cut(s) 1365
NspI RCATGY 1 cut(s) 1144
NspV TTCGAA 1 cut(s) 1647
PagI TCATGA 1 cut(s) 1122
PaqCI CACCTGC 1 cut(s) 151
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PctI GAATGC 2 cut(s) 1242, 1562
PfeI GAWTC 4 cut(s) 322, 636, 788, 1126
PflFI GACNNNGTC 1 cut(s) 413
PleI GAGTC 5 cut(s) 239, 324, 339, 408, 1581
PpsI GAGTC 5 cut(s) 239, 324, 339, 408, 1581
PpuMI RGGWCCY 2 cut(s) 362, 797
PshBI ATTAAT 1 cut(s) 1394
Psp5II RGGWCCY 2 cut(s) 362, 797
Psp6I CCWGG 3 cut(s) 226, 460, 791
PspGI CCWGG 3 cut(s) 226, 460, 791
PspN4I GGNNCC 4 cut(s) 364, 459, 798, 1377
PspOMI GGGCCC 1 cut(s) 457
PspPI GGNCC 5 cut(s) 362, 389, 457, 458, 797
PspPPI RGGWCCY 2 cut(s) 362, 797
PstNI CAGNNNCTG 1 cut(s) 325
PsuI RGATCY 1 cut(s) 1447
PsyI GACNNNGTC 1 cut(s) 413
RsaI GTAC 4 cut(s) 849, 1008, 1147, 1377
RsaNI GTAC 4 cut(s) 848, 1007, 1146, 1376
RseI CAYNNNNRTG 1 cut(s) 1567
SacII CCGCGG 1 cut(s) 238
SaqAI TTAA 3 cut(s) 297, 617, 1394
Sau3AI GATC 4 cut(s) 671, 1339, 1347, 1447
Sau96I GGNCC 5 cut(s) 362, 389, 457, 458, 797
ScaI AGTACT 1 cut(s) 1008
SchI GAGTC 5 cut(s) 240, 325, 340, 409, 1582
ScrFI CCNGG 3 cut(s) 228, 462, 793
SduI GDGCHC 4 cut(s) 461, 655, 1498, 1555
SfaNI GCATC 3 cut(s) 253, 1350, 1469
SfcI CTRYAG 1 cut(s) 816
Sfr303I CCGCGG 1 cut(s) 238
SfuI TTCGAA 1 cut(s) 1647
SgrBI CCGCGG 1 cut(s) 238
SinI GGWCC 2 cut(s) 362, 797
SmiMI CAYNNNNRTG 1 cut(s) 1567
SmlI CTYRAG 3 cut(s) 1244, 1421, 1661
SmoI CTYRAG 3 cut(s) 1244, 1421, 1661
Sse9I AATT 3 cut(s) 510, 1395, 1649
SsiI CCGC 9 cut(s) 174, 207, 235, 237, 278, 366, 550, 1476, 1642
SspMI CTAG 2 cut(s) 311, 546
StyD4I CCNGG 3 cut(s) 226, 460, 791
StyI CCWWGG 5 cut(s) 158, 453, 990, 1104, 1310
TaaI ACNGT 4 cut(s) 415, 469, 1006, 1150
TaqI TCGA 4 cut(s) 408, 1216, 1234, 1647
TaqII GACCGA 1 cut(s) 413
TasI AATT 3 cut(s) 510, 1395, 1649
TatI WGTACW 2 cut(s) 847, 1006
TauI GCSGC 2 cut(s) 209, 240
TfiI GAWTC 4 cut(s) 322, 636, 788, 1126
Tru1I TTAA 3 cut(s) 297, 617, 1394
Tru9I TTAA 3 cut(s) 297, 617, 1394
TscAI CASTG 8 cut(s) 357, 376, 541, 613, 829, 1503, 1560, 1574
TseFI GTSAC 3 cut(s) 906, 1248, 1399
Tsp45I GTSAC 3 cut(s) 906, 1248, 1399
TspDTI ATGAA 6 cut(s) 17, 353, 487, 636, 683, 797
TspRI CASTG 8 cut(s) 357, 376, 541, 613, 829, 1503, 1560, 1574
Tth111I GACNNNGTC 1 cut(s) 413
VneI GTGCAC 1 cut(s) 1494
VpaK11BI GGWCC 2 cut(s) 362, 797
VspI ATTAAT 1 cut(s) 1394
XceI RCATGY 1 cut(s) 1144
XspI CTAG 2 cut(s) 311, 546
ZrmI AGTACT 1 cut(s) 1008
Zsp2I ATGCAT 1 cut(s) 1365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.