Rw6G013090

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001733
Physical Location & Seq
Reverse (-)
24067843 .. 24073707
5865 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G013090.1

Sequence Viewer

Length: 1626 bp
ATGCAAGCTAGAACCCTCCACCTCATTTCTCACTCTCTCTCTTCTCAACCTTGTTGTCTCTTCTCCACTACTCCTCCACCTTCTTCCTCCCTCTTCTTCTTCAACCACACACCTCGATTTCCGAGCTCACGCTCACGCTGCTTCTCCTCCTCATCCTCAGCTCCAATACGACGCCGTCCGATTCGCTCTGTAATCGCCTGCATTGGCGATGGCGGGGACATTTCGGGGTCTGGAGAAGCTGCGGAGGTTAAAGGAGGAGAGTTGGAGAGTCAGAGTATATGGAATCAGATGAAGGAGATTGTCATGTTTACTGGGCCGGCTACCGGGATCTGGATCAGCGGCCCACTTATGAGTCTCATCGACACCGTCGTCGTCGGTCAGGGAAGCTCCATTGAGCTTGCCGCATTAGGTCCTGGAACTGTAATGTGCGATAATATGAGCTATGTTTTCATGTTCCTTTCTATTGCAACTTCCAATATCGTCGCTACTTCCCTTGCAAAAGGGGACAAAAATGAAGTGCAACATCACATAGCCACCTTGCTCTTTGTTGCATTGACCTGTGGCTTCTTAATGCTTTTATTTACAAGATTCTTCGGCTCATGGGCACTGACTGCTTTTACTGGATCGAAGAACATAGATATTATTCCAGCTGCAAATACATACGTTCAGATACGGAGTTTGGCATGGCCCGCTGTTCTTGTTGGTTGGGTTACCCAGAGTGCAAGTCTTGGCATGAAAGATTCATGGGGTCCTTTGAAGGCCTTAGCTGTAGCCAGTGCTATTAATGGCCTTGGTGATATACTACTCTGCAGTGTTTTAGGCTATGGTATTGCTGGTGCAGCATGGGCGACAATGCTGTCACAGATTGTTGCAGGATACATGATGATTGAATCTTTGAACAAGAAAGGATACAACTCTTATGCTATTTCTGTTCCCTCACCCAAAGAACTTCTAACACTAATTGGGCTTGCTGCTCCAGTGTTGGTGACAATGATGTCTAAGGTTGCTTTCTACTCACTCATTGTATATTTTGCAACATCTATGGGCACATATACAATGGCTGCTCATCAGGTCATGATTCAAACATTCATGATCTGTACGGTGTGGGGCGAACCTCTCTCTCAAACGGCACAGTCATTTATGCCTGAGTTGATATATGGAGTAAATCGTAGTTTGCCCAAGGCTCGAATGTTGCTCAAGTCACTTGTCATCATTGGAGCAATCCTTGGTTTGGCTCTTGGAACTGTTGGAACATCTGTTCCATGGTTTTTTCCTAACATCTTTACACCTGATCAGAAGATCATACAGGAGATGCACAAACTACTGATACCATTTTTTTTGGCATTGGCTGTGACACCCGTCATTCTCAGCTTTGAGGGAACATTGCTGGCTGGACGAGATCTTAGATTCATTAGTCTATCAATGAGTAGCTGCTTCTCTGTAGGTGCACTTCTGCTGCTGCTTGTGAGTAGCCGAGGATGTGGTTTAGTAGGCTGCTGGTGGGCACTTGTAGCATTTCAATGGGCTCGGCTTTTCCTTTCTTTACGACGCCTTATATCACCTGATGGCATACTCTACTCTGAAGATATGAGCCGGTATAAACTGGAAGAAGTCAGAGCTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

541

Amino Acids

58.44

Weight (kDa)

8.91

Isoelectric Point (pI)

46.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 115 - 262 2e-13 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 368, 856, 994
AciI CCGC 5 cut(s) 213, 242, 339, 402, 690
AclWI GGATC 3 cut(s) 335, 341, 631
AcuI CTGAAG 1 cut(s) 1602
AcyI GRCGYC 2 cut(s) 172, 1549
AfaI GTAC 1 cut(s) 1099
AfiI CCNNNNNNNGG 3 cut(s) 323, 330, 1231
AgsI TTSAA 6 cut(s) 103, 757, 890, 898, 1082, 1520
AjnI CCWGG 1 cut(s) 412
AloI GAACNNNNNNTCC 4 cut(s) 1242, 1243, 1274, 1275
Alw21I GWGCWC 2 cut(s) 128, 1450
Alw26I GTCTC 2 cut(s) 62, 359
Alw44I GTGCAC 1 cut(s) 1446
AlwI GGATC 3 cut(s) 335, 341, 631
AoxI GGCC 5 cut(s) 314, 340, 686, 759, 787
ApaLI GTGCAC 1 cut(s) 1446
AseI ATTAAT 1 cut(s) 783
AspS9I GGNCC 5 cut(s) 314, 341, 410, 687, 749
AsuC2I CCSGG 1 cut(s) 325
AsuHPI GGTGA 4 cut(s) 806, 930, 997, 1551
AvaII GGWCC 2 cut(s) 410, 749
BaeGI GKGCMC 4 cut(s) 607, 1049, 1450, 1507
BanII GRGCYC 2 cut(s) 128, 1528
BarI GAAGNNNNNNTAC 2 cut(s) 1434, 1466
Bbv12I GWGCWC 2 cut(s) 128, 1450
BbvCI CCTCAGC 1 cut(s) 157
BccI CCATC 2 cut(s) 203, 1559
BceAI ACGGC 2 cut(s) 159, 1143
BciT130I CCWGG 1 cut(s) 414
BciVI GTATCC 2 cut(s) 869, 902
BclI TGATCA 1 cut(s) 1291
BcnI CCSGG 1 cut(s) 325
BcoDI GTCTC 2 cut(s) 62, 359
BfaI CTAG 1 cut(s) 9
BfmI CTRYAG 3 cut(s) 768, 808, 1440
BfuI GTATCC 2 cut(s) 869, 902
BglII AGATCT 1 cut(s) 1399
Bme1390I CCNGG 2 cut(s) 325, 414
Bme18I GGWCC 2 cut(s) 410, 749
BmgT120I GGNCC 5 cut(s) 314, 341, 410, 687, 749
BmiI GGNNCC 1 cut(s) 750
BmrFI CCNGG 2 cut(s) 325, 414
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 1 cut(s) 1302
BmuI ACTGGG 1 cut(s) 321
BoxI GACNNNNGTC 1 cut(s) 1358
BpmI CTGGAG 2 cut(s) 252, 960
Bpu10I CCTNAGC 2 cut(s) 157, 763
BpuEI CTTGAG 1 cut(s) 1181
BpuMI CCSGG 1 cut(s) 325
BsaBI GATNNNNATC 1 cut(s) 332
BsaHI GRCGYC 2 cut(s) 172, 1549
BsaJI CCNNGG 5 cut(s) 790, 1179, 1225, 1262, 1474
BsaXI ACNNNNNCTCC 2 cut(s) 58, 88
Bsc4I CCNNNNNNNGG 3 cut(s) 323, 330, 1231
Bse118I RCCGGY 2 cut(s) 316, 1593
Bse1I ACTGG 5 cut(s) 316, 625, 774, 977, 1608
Bse3DI GCAATG 1 cut(s) 1382
Bse8I GATNNNNATC 1 cut(s) 332
BseBI CCWGG 1 cut(s) 414
BseDI CCNNGG 5 cut(s) 790, 1179, 1225, 1262, 1474
BseGI GGATG 2 cut(s) 152, 1484
BseJI GATNNNNATC 1 cut(s) 332
BseLI CCNNNNNNNGG 3 cut(s) 323, 330, 1231
BseMI GCAATG 1 cut(s) 1382
BseMII CTCAG 3 cut(s) 171, 1137, 1381
BseNI ACTGG 5 cut(s) 316, 625, 774, 977, 1608
BseRI GAGGAG 4 cut(s) 63, 136, 139, 270
BseSI GKGCMC 4 cut(s) 607, 1049, 1450, 1507
BsgI GTGCAG 1 cut(s) 858
BshFI GGCC 5 cut(s) 316, 342, 688, 761, 789
BsiHKAI GWGCWC 2 cut(s) 128, 1450
BsiSI CCGG 3 cut(s) 317, 324, 1594
BslFI GGGAC 2 cut(s) 230, 518
BslI CCNNNNNNNGG 3 cut(s) 323, 330, 1231
BsmAI GTCTC 2 cut(s) 62, 359
BsmFI GGGAC 2 cut(s) 230, 518
BsnI GGCC 5 cut(s) 316, 342, 688, 761, 789
Bsp1286I GDGCHC 6 cut(s) 128, 607, 1049, 1450, 1507, 1528
Bsp143I GATC 7 cut(s) 327, 333, 623, 1092, 1291, 1299, 1399
Bsp19I CCATGG 1 cut(s) 1262
BspACI CCGC 5 cut(s) 213, 242, 339, 402, 690
BspANI GGCC 5 cut(s) 316, 342, 688, 761, 789
BspCNI CTCAG 3 cut(s) 170, 1138, 1380
BspHI TCATGA 2 cut(s) 1074, 1089
BspLI GGNNCC 1 cut(s) 750
BspMAI CTGCAG 1 cut(s) 812
BspPI GGATC 3 cut(s) 335, 341, 631
BsrDI GCAATG 1 cut(s) 1382
BsrFI RCCGGY 2 cut(s) 316, 1593
BsrI ACTGG 5 cut(s) 316, 625, 774, 977, 1608
BssAI RCCGGY 2 cut(s) 316, 1593
BssECI CCNNGG 5 cut(s) 790, 1179, 1225, 1262, 1474
BssMI GATC 7 cut(s) 327, 333, 623, 1092, 1291, 1299, 1399
BssNI GRCGYC 2 cut(s) 172, 1549
BssT1I CCWWGG 4 cut(s) 790, 1179, 1225, 1262
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 5 cut(s) 367, 421, 1102, 1134, 1246
Bst6I CTCTTC 3 cut(s) 46, 65, 98
BstACI GRCGYC 2 cut(s) 172, 1549
BstAPI GCANNNNNTGC 1 cut(s) 611
BstC8I GCNNGC 7 cut(s) 6, 199, 318, 399, 690, 969, 1389
BstDEI CTNAG 6 cut(s) 157, 763, 999, 1146, 1367, 1403
BstDSI CCRYGG 1 cut(s) 1262
BstEII GGTNACC 1 cut(s) 709
BstF5I GGATG 2 cut(s) 152, 1484
BstKTI GATC 7 cut(s) 330, 336, 626, 1095, 1294, 1302, 1402
BstMAI GTCTC 2 cut(s) 62, 359
BstMBI GATC 7 cut(s) 327, 333, 623, 1092, 1291, 1299, 1399
BstMWI GCNNNNNNNGC 6 cut(s) 138, 611, 689, 839, 845, 1511
BstNI CCWGG 1 cut(s) 414
BstPAI GACNNNNGTC 1 cut(s) 1358
BstPI GGTNACC 1 cut(s) 709
BstSCI CCNGG 2 cut(s) 323, 412
BstSFI CTRYAG 3 cut(s) 768, 808, 1440
BstSLI GKGCMC 4 cut(s) 607, 1049, 1450, 1507
BstX2I RGATCY 2 cut(s) 327, 1399
BstYI RGATCY 2 cut(s) 327, 1399
BsuI GTATCC 2 cut(s) 869, 902
BsuRI GGCC 5 cut(s) 316, 342, 688, 761, 789
BtgI CCRYGG 1 cut(s) 1262
BtgZI GCGATG 1 cut(s) 222
BtsCI GGATG 2 cut(s) 152, 1484
BtsI GCAGTG 1 cut(s) 817
BtsIMutI CAGTG 4 cut(s) 605, 781, 817, 984
Cac8I GCNNGC 7 cut(s) 6, 199, 318, 399, 690, 969, 1389
CciI TCATGA 2 cut(s) 1074, 1089
Cfr10I RCCGGY 2 cut(s) 316, 1593
Cfr13I GGNCC 5 cut(s) 314, 341, 410, 687, 749
CseI GACGC 2 cut(s) 180, 1557
Csp6I GTAC 1 cut(s) 1098
CviQI GTAC 1 cut(s) 1098
DdeI CTNAG 6 cut(s) 157, 763, 999, 1146, 1367, 1403
DpnI GATC 7 cut(s) 329, 335, 625, 1094, 1293, 1301, 1401
DpnII GATC 7 cut(s) 327, 333, 623, 1092, 1291, 1299, 1399
DrdI GACNNNNNNGTC 3 cut(s) 368, 856, 994
DseDI GACNNNNNNGTC 3 cut(s) 368, 856, 994
Eam1104I CTCTTC 3 cut(s) 46, 65, 98
EarI CTCTTC 3 cut(s) 46, 65, 98
Ecl136II GAGCTC 1 cut(s) 126
Eco130I CCWWGG 4 cut(s) 790, 1179, 1225, 1262
Eco147I AGGCCT 1 cut(s) 761
Eco24I GRGCYC 2 cut(s) 128, 1528
Eco47I GGWCC 2 cut(s) 410, 749
Eco53kI GAGCTC 1 cut(s) 126
Eco57I CTGAAG 1 cut(s) 1602
Eco91I GGTNACC 1 cut(s) 709
EcoICRI GAGCTC 1 cut(s) 126
EcoO109I RGGNCCY 2 cut(s) 410, 749
EcoO65I GGTNACC 1 cut(s) 709
EcoRII CCWGG 1 cut(s) 412
EcoT14I CCWWGG 4 cut(s) 790, 1179, 1225, 1262
EcoT38I GRGCYC 2 cut(s) 128, 1528
ErhI CCWWGG 4 cut(s) 790, 1179, 1225, 1262
FaqI GGGAC 2 cut(s) 230, 518
FauI CCCGC 2 cut(s) 206, 697
FbaI TGATCA 1 cut(s) 1291
FokI GGATG 2 cut(s) 139, 1491
FriOI GRGCYC 2 cut(s) 128, 1528
FspBI CTAG 1 cut(s) 9
GsuI CTGGAG 2 cut(s) 252, 960
HaeIII GGCC 5 cut(s) 316, 342, 688, 761, 789
HapII CCGG 3 cut(s) 317, 324, 1594
HgaI GACGC 2 cut(s) 180, 1557
Hin1I GRCGYC 2 cut(s) 172, 1549
HinfI GANTC 9 cut(s) 181, 268, 283, 352, 588, 740, 890, 1078, 1407
HpaII CCGG 3 cut(s) 317, 324, 1594
HphI GGTGA 4 cut(s) 806, 930, 997, 1551
Hpy166II GTNNAC 2 cut(s) 309, 1448
Hpy188I TCNGA 8 cut(s) 123, 180, 273, 288, 669, 1296, 1582, 1616
Hpy188III TCNNGA 4 cut(s) 231, 331, 1075, 1090
Hpy8I GTNNAC 2 cut(s) 309, 1448
Hpy99I CGWCG 6 cut(s) 174, 371, 374, 377, 485, 1551
HpyAV CCTTC 3 cut(s) 90, 286, 751
HpyCH4III ACNGT 5 cut(s) 367, 421, 1102, 1134, 1246
HpyCH4IV ACGT 1 cut(s) 663
HpyF10VI GCNNNNNNNGC 6 cut(s) 138, 611, 689, 839, 845, 1511
HpyF3I CTNAG 6 cut(s) 157, 763, 999, 1146, 1367, 1403
HpySE526I ACGT 1 cut(s) 663
Hsp92I GRCGYC 2 cut(s) 172, 1549
KroI GCCGGC 1 cut(s) 316
KroNI GCCGGC 1 cut(s) 318
Ksp22I TGATCA 1 cut(s) 1291
Kzo9I GATC 7 cut(s) 327, 333, 623, 1092, 1291, 1299, 1399
LmnI GCTCC 4 cut(s) 166, 392, 979, 1217
LweI GCATC 1 cut(s) 1302
MaeI CTAG 1 cut(s) 9
MaeII ACGT 1 cut(s) 663
MaeIII GTNAC 5 cut(s) 709, 858, 985, 1200, 1351
MalI GATC 7 cut(s) 329, 335, 625, 1094, 1293, 1301, 1401
MboI GATC 7 cut(s) 327, 333, 623, 1092, 1291, 1299, 1399
MflI RGATCY 2 cut(s) 327, 1399
MhlI GDGCHC 6 cut(s) 128, 607, 1049, 1450, 1507, 1528
MluCI AATT 1 cut(s) 960
MlyI GAGTC 2 cut(s) 277, 361
MmeI TCCRAC 2 cut(s) 243, 1228
MroNI GCCGGC 1 cut(s) 316
MseI TTAA 3 cut(s) 249, 569, 783
MslI CAYNNNNRTG 1 cut(s) 1519
MspA1I CMGCKG 3 cut(s) 339, 650, 692
MspI CCGG 3 cut(s) 317, 324, 1594
MspR9I CCNGG 2 cut(s) 325, 414
MvaI CCWGG 1 cut(s) 414
MwoI GCNNNNNNNGC 6 cut(s) 138, 611, 689, 839, 845, 1511
NaeI GCCGGC 1 cut(s) 318
NciI CCSGG 1 cut(s) 325
NcoI CCATGG 1 cut(s) 1262
NdeII GATC 7 cut(s) 327, 333, 623, 1092, 1291, 1299, 1399
NgoMIV GCCGGC 1 cut(s) 316
NlaIV GGNNCC 1 cut(s) 750
NmeAIII GCCGAG 2 cut(s) 1499, 1507
NmuCI GTSAC 4 cut(s) 858, 985, 1200, 1351
PagI TCATGA 2 cut(s) 1074, 1089
PceI AGGCCT 1 cut(s) 761
PcsI WCGNNNNNNNCGW 1 cut(s) 366
PdiI GCCGGC 1 cut(s) 318
PfeI GAWTC 7 cut(s) 181, 283, 588, 740, 890, 1078, 1407
PflFI GACNNNGTC 2 cut(s) 174, 365
PfoI TCCNGGA 1 cut(s) 412
PleI GAGTC 2 cut(s) 276, 360
PpsI GAGTC 2 cut(s) 276, 360
PpuMI RGGWCCY 2 cut(s) 410, 749
PshAI GACNNNNGTC 1 cut(s) 1358
PshBI ATTAAT 1 cut(s) 783
Psp124BI GAGCTC 1 cut(s) 128
Psp5II RGGWCCY 2 cut(s) 410, 749
Psp6I CCWGG 1 cut(s) 412
PspEI GGTNACC 1 cut(s) 709
PspGI CCWGG 1 cut(s) 412
PspN4I GGNNCC 1 cut(s) 750
PspPI GGNCC 5 cut(s) 314, 341, 410, 687, 749
PspPPI RGGWCCY 2 cut(s) 410, 749
PstI CTGCAG 1 cut(s) 812
PsuI RGATCY 2 cut(s) 327, 1399
PsyI GACNNNGTC 2 cut(s) 174, 365
PvuII CAGCTG 1 cut(s) 650
RsaI GTAC 1 cut(s) 1099
RsaNI GTAC 1 cut(s) 1098
RseI CAYNNNNRTG 1 cut(s) 1519
SacI GAGCTC 1 cut(s) 128
SaqAI TTAA 3 cut(s) 249, 569, 783
Sau3AI GATC 7 cut(s) 327, 333, 623, 1092, 1291, 1299, 1399
Sau96I GGNCC 5 cut(s) 314, 341, 410, 687, 749
SchI GAGTC 2 cut(s) 277, 361
ScrFI CCNGG 2 cut(s) 325, 414
SduI GDGCHC 6 cut(s) 128, 607, 1049, 1450, 1507, 1528
SfaNI GCATC 1 cut(s) 1302
SfcI CTRYAG 3 cut(s) 768, 808, 1440
SinI GGWCC 2 cut(s) 410, 749
SmiMI CAYNNNNRTG 1 cut(s) 1519
SmlI CTYRAG 1 cut(s) 1196
SmoI CTYRAG 1 cut(s) 1196
Sse9I AATT 1 cut(s) 960
SseBI AGGCCT 1 cut(s) 761
SsiI CCGC 5 cut(s) 213, 242, 339, 402, 690
SspMI CTAG 1 cut(s) 9
SstI GAGCTC 1 cut(s) 128
StuI AGGCCT 1 cut(s) 761
StyD4I CCNGG 2 cut(s) 323, 412
StyI CCWWGG 4 cut(s) 790, 1179, 1225, 1262
TaaI ACNGT 5 cut(s) 367, 421, 1102, 1134, 1246
TaiI ACGT 1 cut(s) 666
TaqI TCGA 4 cut(s) 115, 360, 626, 1186
TaqII GACCGA 1 cut(s) 365
TasI AATT 1 cut(s) 960
TauI GCSGC 2 cut(s) 342, 404
TfiI GAWTC 7 cut(s) 181, 283, 588, 740, 890, 1078, 1407
Tru1I TTAA 3 cut(s) 249, 569, 783
Tru9I TTAA 3 cut(s) 249, 569, 783
TscAI CASTG 4 cut(s) 612, 781, 817, 984
TseFI GTSAC 4 cut(s) 858, 985, 1200, 1351
Tsp45I GTSAC 4 cut(s) 858, 985, 1200, 1351
TspDTI ATGAA 7 cut(s) 305, 439, 528, 732, 749, 1078, 1399
TspGWI ACGGA 1 cut(s) 688
TspRI CASTG 4 cut(s) 612, 781, 817, 984
Tth111I GACNNNGTC 2 cut(s) 174, 365
VneI GTGCAC 1 cut(s) 1446
VpaK11BI GGWCC 2 cut(s) 410, 749
VspI ATTAAT 1 cut(s) 783
XspI CTAG 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.