Rroxscaffold_7G00201060

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001733
Physical Location & Seq
Reverse (-)
48536043 .. 48541943
5901 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00201060.1

Sequence Viewer

Length: 1635 bp
ATGCAAGCTAGAACCCTCCACCTCATTTCTCACTCTCTCTCTTCTCAACCTTCTTCTTGTCTCTTCTCCACTACTCCTCCACCTTCTTCCTCTTTCTTCTTCAACCACACACCTCGATTTCCCAGCTCACGCTGCTTCTCCTCCTCCTCCTCCTCAGCTCCAATACGACGCCGTCCGATTCGGCCTGTAATCGCTTGCATTGGCGATGGCGGGGCCGGAATGGACGACATTTCGAGCTCTGGAGAAGCTGCGGAGGTTACAGGAGGAGAGTTGGAGAGTCAGAGTATATGGAATCAGATGAAGGAGATTGTCATGTTTACTGGGCCGGCTACCGGGATCTGGATCAGCGGCCCACTTATGAGTCTCATCGACACTGTTGTCGTCGGTCAGGGAAGCTCCATTGAGCTTGCTGCTTTAGGTCCTGGAACTGTAATGTGCGATAATATGAGCTATGTTTTCATGTTCCTTTCTATTGCAACTTCCAATATCGTCGCTACTTCCCTTGCAAAAGGGGACAAAAATGAAGTGCAACATCACATATCCACCTTGCTCTTTGTTGCATTGACCTGTGGCTTCTTAATGCTTCTATTTACAAGATTCTTCGGCTCATGGGCACTGACTGCTTTTACTGGATCGAAGAACGTAGATATTATTCCAGCAGCAAATACATACGTTCAGATACGGAGTTTGGCATGGCCTGCAGTTCTTGTTGGTTGGGTTACCCAGAGTGCAAGTCTTGGCATGAAAGATTCATGGGGTCCTTTGAAGGCCTTAGCTGTAGCCAGTGCTATTAATGGCCTTGGTGATATACTACTCTGCAGCGTTTTAGGCTATGGTATTGCTGGTGCAGCATGGGCGACAATGCTGTCACAGATTGTTGCAGGATACATGATGATTGAATCTTTGAACAAGAAAGGATACAACTCTTATGCCATTTCTGTTCCCTCACCCAAAGAACTTCTAACACTAATTGGGCTTGCTGCTCCAGTGTTGGTGACAATGATGTCTAAGGTTGCTTTCTACTCGCTCATTGTATATTTTGCAACATCTATGGGCACATATACAATGGCTGCTCATCAGGTCATGATTCAAACATTCATGATCTGTACGGTGTGGGGCGAACCTCTCTCTCAAACGGCACAGTCATTTATGCCTGAGTTGATATATGGAGTAAATCGTAGTTTGCCCAAGGCTCGAATGTTGCTCAAGTCACTTGTCATCATTGGAGCAATCCTTGGTTTGGCTCTTGGAACTGTTGGAACATCTGTTCCATGGTTTTTTCCTAACATCTTTACACCTGATCAGAAGATCATACAGGAGATGCACAAACTACTGATACCATTTTTTTTGGCATTGGCTGTGACACCTGCCATTCTCAGCTTTGAGGGAACATTGCTGGCTGGACGAGATCTTAGATTCATTAGTCTATCAATGAGTAGCTGCTTCTCTTTAGGTGCACTTCTGCTGCTGCTTGTGAGTAGCCGAGGATGTGGTTTAGTAGGCTGCTGGTGGGCACTTGTAGCATTTCAATGGGCTCGGCTTTTCCTTTCTCTACGACGCCTTATATCACCTGATGGCATACTCTACTCTGAAGATATGAGCCGGTATAAACTGGAAGAAGTCAGAGCTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

544

Amino Acids

58.62

Weight (kDa)

8.62

Isoelectric Point (pI)

46.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 118 - 265 1.4e-13 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1375
AasI GACNNNNNNGTC 3 cut(s) 377, 865, 1003
Acc36I ACCTGC 1 cut(s) 1375
AciI CCGC 3 cut(s) 210, 251, 348
AclWI GGATC 3 cut(s) 344, 350, 640
AcuI CTGAAG 1 cut(s) 1611
AcyI GRCGYC 2 cut(s) 169, 1558
AfaI GTAC 1 cut(s) 1108
AfiI CCNNNNNNNGG 3 cut(s) 332, 339, 1240
AgsI TTSAA 6 cut(s) 103, 766, 899, 907, 1091, 1529
AjnI CCWGG 1 cut(s) 421
AloI GAACNNNNNNTCC 4 cut(s) 1251, 1252, 1283, 1284
Alw21I GWGCWC 2 cut(s) 239, 1459
Alw26I GTCTC 2 cut(s) 65, 368
Alw44I GTGCAC 1 cut(s) 1455
AlwI GGATC 3 cut(s) 344, 350, 640
AoxI GGCC 7 cut(s) 182, 213, 323, 349, 695, 768, 796
ApaLI GTGCAC 1 cut(s) 1455
AseI ATTAAT 1 cut(s) 792
AspS9I GGNCC 5 cut(s) 213, 323, 350, 419, 758
AsuC2I CCSGG 1 cut(s) 334
AsuHPI GGTGA 4 cut(s) 815, 939, 1006, 1560
AvaII GGWCC 2 cut(s) 419, 758
BaeGI GKGCMC 4 cut(s) 616, 1058, 1459, 1516
BanII GRGCYC 2 cut(s) 239, 1537
Bbv12I GWGCWC 2 cut(s) 239, 1459
BbvCI CCTCAGC 1 cut(s) 154
BccI CCATC 2 cut(s) 200, 1568
BceAI ACGGC 2 cut(s) 156, 1152
BciT130I CCWGG 1 cut(s) 423
BciVI GTATCC 2 cut(s) 878, 911
BclI TGATCA 1 cut(s) 1300
BcnI CCSGG 1 cut(s) 334
BcoDI GTCTC 2 cut(s) 65, 368
BfaI CTAG 1 cut(s) 9
BfmI CTRYAG 3 cut(s) 699, 777, 817
BfuAI ACCTGC 1 cut(s) 1375
BfuI GTATCC 2 cut(s) 878, 911
BglII AGATCT 1 cut(s) 1408
Bme1390I CCNGG 2 cut(s) 334, 423
Bme18I GGWCC 2 cut(s) 419, 758
BmgT120I GGNCC 5 cut(s) 213, 323, 350, 419, 758
BmiI GGNNCC 2 cut(s) 214, 759
BmrFI CCNGG 2 cut(s) 334, 423
BmrI ACTGGG 1 cut(s) 330
BmsI GCATC 1 cut(s) 1311
BmuI ACTGGG 1 cut(s) 330
BpmI CTGGAG 2 cut(s) 261, 969
Bpu10I CCTNAGC 2 cut(s) 154, 772
BpuEI CTTGAG 1 cut(s) 1190
BpuMI CCSGG 1 cut(s) 334
BsaBI GATNNNNATC 1 cut(s) 341
BsaHI GRCGYC 2 cut(s) 169, 1558
BsaJI CCNNGG 5 cut(s) 799, 1188, 1234, 1271, 1483
BsaXI ACNNNNNCTCC 2 cut(s) 61, 91
Bsc4I CCNNNNNNNGG 3 cut(s) 332, 339, 1240
Bse118I RCCGGY 2 cut(s) 325, 1602
Bse1I ACTGG 5 cut(s) 325, 634, 783, 986, 1617
Bse3DI GCAATG 1 cut(s) 1391
Bse8I GATNNNNATC 1 cut(s) 341
BseBI CCWGG 1 cut(s) 423
BseDI CCNNGG 5 cut(s) 799, 1188, 1234, 1271, 1483
BseGI GGATG 1 cut(s) 1493
BseJI GATNNNNATC 1 cut(s) 341
BseLI CCNNNNNNNGG 3 cut(s) 332, 339, 1240
BseMI GCAATG 1 cut(s) 1391
BseMII CTCAG 3 cut(s) 168, 1146, 1390
BseNI ACTGG 5 cut(s) 325, 634, 783, 986, 1617
BseRI GAGGAG 7 cut(s) 66, 130, 133, 136, 139, 142, 279
BseSI GKGCMC 4 cut(s) 616, 1058, 1459, 1516
BseYI CCCAGC 1 cut(s) 122
BsgI GTGCAG 1 cut(s) 867
BshFI GGCC 7 cut(s) 184, 215, 325, 351, 697, 770, 798
BsiHKAI GWGCWC 2 cut(s) 239, 1459
BsiSI CCGG 4 cut(s) 216, 326, 333, 1603
BslFI GGGAC 1 cut(s) 527
BslI CCNNNNNNNGG 3 cut(s) 332, 339, 1240
BsmAI GTCTC 2 cut(s) 65, 368
BsmFI GGGAC 1 cut(s) 527
BsnI GGCC 7 cut(s) 184, 215, 325, 351, 697, 770, 798
Bsp1286I GDGCHC 6 cut(s) 239, 616, 1058, 1459, 1516, 1537
Bsp143I GATC 7 cut(s) 336, 342, 632, 1101, 1300, 1308, 1408
Bsp19I CCATGG 1 cut(s) 1271
BspACI CCGC 3 cut(s) 210, 251, 348
BspANI GGCC 7 cut(s) 184, 215, 325, 351, 697, 770, 798
BspCNI CTCAG 3 cut(s) 167, 1147, 1389
BspHI TCATGA 2 cut(s) 1083, 1098
BspLI GGNNCC 2 cut(s) 214, 759
BspMAI CTGCAG 2 cut(s) 703, 821
BspMI ACCTGC 1 cut(s) 1375
BspPI GGATC 3 cut(s) 344, 350, 640
BsrDI GCAATG 1 cut(s) 1391
BsrFI RCCGGY 2 cut(s) 325, 1602
BsrI ACTGG 5 cut(s) 325, 634, 783, 986, 1617
BssAI RCCGGY 2 cut(s) 325, 1602
BssECI CCNNGG 5 cut(s) 799, 1188, 1234, 1271, 1483
BssMI GATC 7 cut(s) 336, 342, 632, 1101, 1300, 1308, 1408
BssNI GRCGYC 2 cut(s) 169, 1558
BssT1I CCWWGG 4 cut(s) 799, 1188, 1234, 1271
Bst2UI CCWGG 1 cut(s) 423
Bst4CI ACNGT 5 cut(s) 376, 430, 1111, 1143, 1255
Bst6I CTCTTC 2 cut(s) 46, 68
BstACI GRCGYC 2 cut(s) 169, 1558
BstAPI GCANNNNNTGC 2 cut(s) 620, 698
BstC8I GCNNGC 7 cut(s) 6, 196, 327, 408, 699, 978, 1398
BstDEI CTNAG 6 cut(s) 154, 772, 1008, 1155, 1376, 1412
BstDSI CCRYGG 1 cut(s) 1271
BstEII GGTNACC 1 cut(s) 718
BstF5I GGATG 1 cut(s) 1493
BstKTI GATC 7 cut(s) 339, 345, 635, 1104, 1303, 1311, 1411
BstMAI GTCTC 2 cut(s) 65, 368
BstMBI GATC 7 cut(s) 336, 342, 632, 1101, 1300, 1308, 1408
BstMWI GCNNNNNNNGC 7 cut(s) 132, 620, 698, 828, 848, 854, 1520
BstNI CCWGG 1 cut(s) 423
BstPI GGTNACC 1 cut(s) 718
BstSCI CCNGG 2 cut(s) 332, 421
BstSFI CTRYAG 3 cut(s) 699, 777, 817
BstSLI GKGCMC 4 cut(s) 616, 1058, 1459, 1516
BstX2I RGATCY 2 cut(s) 336, 1408
BstYI RGATCY 2 cut(s) 336, 1408
BsuI GTATCC 2 cut(s) 878, 911
BsuRI GGCC 7 cut(s) 184, 215, 325, 351, 697, 770, 798
BtgI CCRYGG 1 cut(s) 1271
BtgZI GCGATG 1 cut(s) 219
BtsCI GGATG 1 cut(s) 1493
BtsIMutI CAGTG 4 cut(s) 372, 614, 790, 993
BveI ACCTGC 1 cut(s) 1375
Cac8I GCNNGC 7 cut(s) 6, 196, 327, 408, 699, 978, 1398
CciI TCATGA 2 cut(s) 1083, 1098
Cfr10I RCCGGY 2 cut(s) 325, 1602
Cfr13I GGNCC 5 cut(s) 213, 323, 350, 419, 758
CseI GACGC 2 cut(s) 177, 1566
Csp6I GTAC 1 cut(s) 1107
CviQI GTAC 1 cut(s) 1107
DdeI CTNAG 6 cut(s) 154, 772, 1008, 1155, 1376, 1412
DpnI GATC 7 cut(s) 338, 344, 634, 1103, 1302, 1310, 1410
DpnII GATC 7 cut(s) 336, 342, 632, 1101, 1300, 1308, 1408
DrdI GACNNNNNNGTC 3 cut(s) 377, 865, 1003
DseDI GACNNNNNNGTC 3 cut(s) 377, 865, 1003
Eam1104I CTCTTC 2 cut(s) 46, 68
EarI CTCTTC 2 cut(s) 46, 68
Ecl136II GAGCTC 1 cut(s) 237
Eco130I CCWWGG 4 cut(s) 799, 1188, 1234, 1271
Eco147I AGGCCT 1 cut(s) 770
Eco24I GRGCYC 2 cut(s) 239, 1537
Eco47I GGWCC 2 cut(s) 419, 758
Eco53kI GAGCTC 1 cut(s) 237
Eco57I CTGAAG 1 cut(s) 1611
Eco91I GGTNACC 1 cut(s) 718
EcoICRI GAGCTC 1 cut(s) 237
EcoO109I RGGNCCY 2 cut(s) 419, 758
EcoO65I GGTNACC 1 cut(s) 718
EcoRII CCWGG 1 cut(s) 421
EcoT14I CCWWGG 4 cut(s) 799, 1188, 1234, 1271
EcoT38I GRGCYC 2 cut(s) 239, 1537
ErhI CCWWGG 4 cut(s) 799, 1188, 1234, 1271
FaqI GGGAC 1 cut(s) 527
FauI CCCGC 1 cut(s) 203
FbaI TGATCA 1 cut(s) 1300
FokI GGATG 1 cut(s) 1500
FriOI GRGCYC 2 cut(s) 239, 1537
FspBI CTAG 1 cut(s) 9
GsaI CCCAGC 1 cut(s) 126
GsuI CTGGAG 2 cut(s) 261, 969
HaeIII GGCC 7 cut(s) 184, 215, 325, 351, 697, 770, 798
HapII CCGG 4 cut(s) 216, 326, 333, 1603
HgaI GACGC 2 cut(s) 177, 1566
Hin1I GRCGYC 2 cut(s) 169, 1558
HinfI GANTC 9 cut(s) 178, 277, 292, 361, 597, 749, 899, 1087, 1416
HpaII CCGG 4 cut(s) 216, 326, 333, 1603
HphI GGTGA 4 cut(s) 815, 939, 1006, 1560
Hpy166II GTNNAC 2 cut(s) 318, 1457
Hpy188I TCNGA 7 cut(s) 177, 282, 297, 678, 1305, 1591, 1625
Hpy188III TCNNGA 4 cut(s) 240, 340, 1084, 1099
Hpy8I GTNNAC 2 cut(s) 318, 1457
Hpy99I CGWCG 4 cut(s) 171, 386, 494, 1560
HpyAV CCTTC 4 cut(s) 60, 93, 295, 760
HpyCH4III ACNGT 5 cut(s) 376, 430, 1111, 1143, 1255
HpyCH4IV ACGT 2 cut(s) 642, 672
HpyF10VI GCNNNNNNNGC 7 cut(s) 132, 620, 698, 828, 848, 854, 1520
HpyF3I CTNAG 6 cut(s) 154, 772, 1008, 1155, 1376, 1412
HpySE526I ACGT 2 cut(s) 642, 672
Hsp92I GRCGYC 2 cut(s) 169, 1558
KroI GCCGGC 1 cut(s) 325
KroNI GCCGGC 1 cut(s) 327
Ksp22I TGATCA 1 cut(s) 1300
Kzo9I GATC 7 cut(s) 336, 342, 632, 1101, 1300, 1308, 1408
LmnI GCTCC 4 cut(s) 163, 401, 988, 1226
LweI GCATC 1 cut(s) 1311
MaeI CTAG 1 cut(s) 9
MaeII ACGT 2 cut(s) 642, 672
MaeIII GTNAC 6 cut(s) 256, 718, 867, 994, 1209, 1360
MalI GATC 7 cut(s) 338, 344, 634, 1103, 1302, 1310, 1410
MboI GATC 7 cut(s) 336, 342, 632, 1101, 1300, 1308, 1408
MflI RGATCY 2 cut(s) 336, 1408
MhlI GDGCHC 6 cut(s) 239, 616, 1058, 1459, 1516, 1537
MluCI AATT 1 cut(s) 969
MlyI GAGTC 2 cut(s) 286, 370
MmeI TCCRAC 2 cut(s) 252, 1237
MroNI GCCGGC 1 cut(s) 325
MseI TTAA 2 cut(s) 578, 792
MslI CAYNNNNRTG 1 cut(s) 1528
MspA1I CMGCKG 1 cut(s) 348
MspI CCGG 4 cut(s) 216, 326, 333, 1603
MspR9I CCNGG 2 cut(s) 334, 423
MvaI CCWGG 1 cut(s) 423
MwoI GCNNNNNNNGC 7 cut(s) 132, 620, 698, 828, 848, 854, 1520
NaeI GCCGGC 1 cut(s) 327
NciI CCSGG 1 cut(s) 334
NcoI CCATGG 1 cut(s) 1271
NdeII GATC 7 cut(s) 336, 342, 632, 1101, 1300, 1308, 1408
NgoMIV GCCGGC 1 cut(s) 325
NlaIV GGNNCC 2 cut(s) 214, 759
NmeAIII GCCGAG 2 cut(s) 1508, 1516
NmuCI GTSAC 4 cut(s) 867, 994, 1209, 1360
PagI TCATGA 2 cut(s) 1083, 1098
PaqCI CACCTGC 1 cut(s) 1375
PceI AGGCCT 1 cut(s) 770
PdiI GCCGGC 1 cut(s) 327
PfeI GAWTC 7 cut(s) 178, 292, 597, 749, 899, 1087, 1416
PflFI GACNNNGTC 1 cut(s) 171
PfoI TCCNGGA 1 cut(s) 421
PleI GAGTC 2 cut(s) 285, 369
PpsI GAGTC 2 cut(s) 285, 369
PpuMI RGGWCCY 2 cut(s) 419, 758
PshBI ATTAAT 1 cut(s) 792
Psp124BI GAGCTC 1 cut(s) 239
Psp5II RGGWCCY 2 cut(s) 419, 758
Psp6I CCWGG 1 cut(s) 421
PspEI GGTNACC 1 cut(s) 718
PspFI CCCAGC 1 cut(s) 122
PspGI CCWGG 1 cut(s) 421
PspN4I GGNNCC 2 cut(s) 214, 759
PspPI GGNCC 5 cut(s) 213, 323, 350, 419, 758
PspPPI RGGWCCY 2 cut(s) 419, 758
PstI CTGCAG 2 cut(s) 703, 821
PsuI RGATCY 2 cut(s) 336, 1408
PsyI GACNNNGTC 1 cut(s) 171
RsaI GTAC 1 cut(s) 1108
RsaNI GTAC 1 cut(s) 1107
RseI CAYNNNNRTG 1 cut(s) 1528
SacI GAGCTC 1 cut(s) 239
SaqAI TTAA 2 cut(s) 578, 792
Sau3AI GATC 7 cut(s) 336, 342, 632, 1101, 1300, 1308, 1408
Sau96I GGNCC 5 cut(s) 213, 323, 350, 419, 758
SchI GAGTC 2 cut(s) 286, 370
ScrFI CCNGG 2 cut(s) 334, 423
SduI GDGCHC 6 cut(s) 239, 616, 1058, 1459, 1516, 1537
SfaNI GCATC 1 cut(s) 1311
SfcI CTRYAG 3 cut(s) 699, 777, 817
SinI GGWCC 2 cut(s) 419, 758
SmiMI CAYNNNNRTG 1 cut(s) 1528
SmlI CTYRAG 1 cut(s) 1205
SmoI CTYRAG 1 cut(s) 1205
Sse9I AATT 1 cut(s) 969
SseBI AGGCCT 1 cut(s) 770
SsiI CCGC 3 cut(s) 210, 251, 348
SspMI CTAG 1 cut(s) 9
SstI GAGCTC 1 cut(s) 239
StuI AGGCCT 1 cut(s) 770
StyD4I CCNGG 2 cut(s) 332, 421
StyI CCWWGG 4 cut(s) 799, 1188, 1234, 1271
TaaI ACNGT 5 cut(s) 376, 430, 1111, 1143, 1255
TaiI ACGT 2 cut(s) 645, 675
TaqI TCGA 5 cut(s) 115, 233, 369, 635, 1195
TaqII GACCGA 1 cut(s) 374
TasI AATT 1 cut(s) 969
TauI GCSGC 1 cut(s) 351
TfiI GAWTC 7 cut(s) 178, 292, 597, 749, 899, 1087, 1416
Tru1I TTAA 2 cut(s) 578, 792
Tru9I TTAA 2 cut(s) 578, 792
TscAI CASTG 4 cut(s) 379, 621, 790, 993
TseFI GTSAC 4 cut(s) 867, 994, 1209, 1360
Tsp45I GTSAC 4 cut(s) 867, 994, 1209, 1360
TspDTI ATGAA 7 cut(s) 314, 448, 537, 741, 758, 1087, 1408
TspGWI ACGGA 1 cut(s) 697
TspRI CASTG 4 cut(s) 379, 621, 790, 993
Tth111I GACNNNGTC 1 cut(s) 171
VneI GTGCAC 1 cut(s) 1455
VpaK11BI GGWCC 2 cut(s) 419, 758
VspI ATTAAT 1 cut(s) 792
XspI CTAG 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.