MD10G1204800.v1.1

Auxin-induced protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009941
Physical Location & Seq
Forward (+)
30376680 .. 30377099
420 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1204800.v1.1.491

Sequence Viewer

Length: 420 bp
ATGAAGCAGCTGATCCGCCGGCTCTCCCGCGTCGCCGACTCATCGCAGTACTGCCTCCTGCGCTCCCCGGCCGACGCCTCCGCCCCCCCGCGCAGAAACGAGTCCTTCCGTGTAAAGCTCCGGCGCCGGAGATCCTCCGCTGGCGAGCAGGTGGCCCCCGAGGGCCACGTCCCGGTCTACGTCGGCGACGAGATGGAGCGGTTCGTGGTCAGTGCCGAGCTCCTGAACCACCCGGTCTTCGTCGAGCTCCTCAACAAGTCGGCACAGGAGTACGGCTACGAGCAGAAGGGAGTCCTCCGGATCCCATGCCACGTGATCGTCTTCGAGCGCGTGCTCGAGGCGCTCCGCCTCGGACAGGCCCCGTCGTGCGACCTGCACGACCTCCTCAACTCGAACTCCGTCGACCTGAGATCCTTGTAG

Protein Analysis

140

Amino Acids

15.75

Weight (kDa)

8.93

Isoelectric Point (pI)

77.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 31 - 115 1.1e-29 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 139
Acc36I ACCTGC 2 cut(s) 139, 381
AccB1I GGYRCC 1 cut(s) 123
AccBSI CCGCTC 1 cut(s) 199
AccI GTMKAC 2 cut(s) 177, 402
AccII CGCG 3 cut(s) 30, 91, 330
AccIII TCCGGA 1 cut(s) 297
AciI CCGC 7 cut(s) 16, 28, 81, 89, 138, 199, 346
AclWI GGATC 5 cut(s) 7, 126, 295, 308, 405
AcoI YGGCCR 1 cut(s) 69
AcvI CACGTG 1 cut(s) 313
AcyI GRCGYC 2 cut(s) 75, 124
AfaI GTAC 2 cut(s) 50, 272
AfiI CCNNNNNNNGG 2 cut(s) 172, 355
AjiI CACGTC 1 cut(s) 169
AluBI AGCT 4 cut(s) 10, 118, 220, 247
AluI AGCT 4 cut(s) 10, 118, 220, 247
Alw21I GWGCWC 3 cut(s) 222, 249, 336
AlwI GGATC 5 cut(s) 7, 126, 295, 308, 405
Ama87I CYCGRG 2 cut(s) 158, 335
Aor13HI TCCGGA 1 cut(s) 297
AoxI GGCC 4 cut(s) 69, 153, 163, 357
ApeKI GCWGC 1 cut(s) 7
AspLEI GCGC 5 cut(s) 63, 93, 126, 330, 343
AspS9I GGNCC 3 cut(s) 154, 163, 358
AsuC2I CCSGG 3 cut(s) 68, 173, 233
AvaI CYCGRG 2 cut(s) 158, 335
BamHI GGATCC 1 cut(s) 300
BanI GGYRCC 1 cut(s) 123
BanII GRGCYC 2 cut(s) 222, 249
BbrPI CACGTG 1 cut(s) 313
BbsI GAAGAC 2 cut(s) 229, 313
Bbv12I GWGCWC 3 cut(s) 222, 249, 336
BbvI GCAGC 1 cut(s) 19
BccI CCATC 1 cut(s) 187
BceAI ACGGC 1 cut(s) 289
BcnI CCSGG 3 cut(s) 68, 173, 233
BfoI RGCGCY 2 cut(s) 127, 344
BfuAI ACCTGC 2 cut(s) 139, 381
BisI GCNGC 1 cut(s) 8
BlsI GCNGC 1 cut(s) 9
BmcAI AGTACT 1 cut(s) 50
Bme1390I CCNGG 3 cut(s) 68, 173, 233
BmeT110I CYCGRG 2 cut(s) 158, 335
BmgBI CACGTC 1 cut(s) 169
BmgT120I GGNCC 3 cut(s) 154, 163, 358
BmiI GGNNCC 4 cut(s) 125, 156, 302, 360
BmrFI CCNGG 3 cut(s) 68, 173, 233
BpiI GAAGAC 2 cut(s) 229, 313
BpuMI CCSGG 3 cut(s) 68, 173, 233
BsaAI YACGTR 1 cut(s) 313
BsaHI GRCGYC 2 cut(s) 75, 124
BsaJI CCNNGG 3 cut(s) 66, 159, 349
BsaWI WCCGGW 1 cut(s) 297
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 355
Bse118I RCCGGY 1 cut(s) 18
BseAI TCCGGA 1 cut(s) 297
BseDI CCNNGG 3 cut(s) 66, 159, 349
BseLI CCNNNNNNNGG 2 cut(s) 172, 355
BseMII CTCAG 1 cut(s) 398
BseRI GAGGAG 2 cut(s) 239, 374
BseX3I CGGCCG 1 cut(s) 69
BseXI GCAGC 1 cut(s) 19
BsgI GTGCAG 1 cut(s) 359
Bsh1236I CGCG 3 cut(s) 30, 91, 330
Bsh1285I CGRYCG 1 cut(s) 72
BshFI GGCC 4 cut(s) 71, 155, 165, 359
BshNI GGYRCC 1 cut(s) 123
BsiEI CGRYCG 1 cut(s) 72
BsiHKAI GWGCWC 3 cut(s) 222, 249, 336
BsiHKCI CYCGRG 2 cut(s) 158, 335
BsiSI CCGG 7 cut(s) 19, 68, 121, 127, 173, 233, 298
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 2 cut(s) 172, 355
BsmFI GGGAC 1 cut(s) 155
BsnI GGCC 4 cut(s) 71, 155, 165, 359
BsoBI CYCGRG 2 cut(s) 158, 335
Bsp1286I GDGCHC 3 cut(s) 222, 249, 336
Bsp13I TCCGGA 1 cut(s) 297
Bsp143I GATC 5 cut(s) 12, 131, 300, 315, 410
BspACI CCGC 7 cut(s) 16, 28, 81, 89, 138, 199, 346
BspANI GGCC 4 cut(s) 71, 155, 165, 359
BspCNI CTCAG 1 cut(s) 399
BspEI TCCGGA 1 cut(s) 297
BspFNI CGCG 3 cut(s) 30, 91, 330
BspLI GGNNCC 4 cut(s) 125, 156, 302, 360
BspMI ACCTGC 2 cut(s) 139, 381
BspPI GGATC 5 cut(s) 7, 126, 295, 308, 405
BspT107I GGYRCC 1 cut(s) 123
BsrBI CCGCTC 1 cut(s) 199
BsrFI RCCGGY 1 cut(s) 18
BssAI RCCGGY 1 cut(s) 18
BssECI CCNNGG 3 cut(s) 66, 159, 349
BssMI GATC 5 cut(s) 12, 131, 300, 315, 410
BssNI GRCGYC 2 cut(s) 75, 124
BstACI GRCGYC 2 cut(s) 75, 124
BstBAI YACGTR 1 cut(s) 313
BstC8I GCNNGC 4 cut(s) 20, 142, 146, 332
BstDEI CTNAG 1 cut(s) 407
BstENI CCTNNNNNAGG 1 cut(s) 353
BstFNI CGCG 3 cut(s) 30, 91, 330
BstH2I RGCGCY 2 cut(s) 127, 344
BstHHI GCGC 5 cut(s) 63, 93, 126, 330, 343
BstKTI GATC 5 cut(s) 15, 134, 303, 318, 413
BstMBI GATC 5 cut(s) 12, 131, 300, 315, 410
BstMCI CGRYCG 1 cut(s) 72
BstMWI GCNNNNNNNGC 2 cut(s) 60, 340
BstSCI CCNGG 3 cut(s) 66, 171, 231
BstUI CGCG 3 cut(s) 30, 91, 330
BstV1I GCAGC 1 cut(s) 19
BstV2I GAAGAC 2 cut(s) 229, 313
BstX2I RGATCY 3 cut(s) 131, 300, 410
BstYI RGATCY 3 cut(s) 131, 300, 410
BstZI CGGCCG 1 cut(s) 69
BsuRI GGCC 4 cut(s) 71, 155, 165, 359
BtgZI GCGATG 1 cut(s) 27
BtrI CACGTC 1 cut(s) 169
BtsIMutI CAGTG 1 cut(s) 217
BveI ACCTGC 2 cut(s) 139, 381
Cac8I GCNNGC 4 cut(s) 20, 142, 146, 332
CfoI GCGC 5 cut(s) 63, 93, 126, 330, 343
Cfr10I RCCGGY 1 cut(s) 18
Cfr13I GGNCC 3 cut(s) 154, 163, 358
CseI GACGC 2 cut(s) 19, 83
Csp6I GTAC 2 cut(s) 49, 271
CviAII CATG 1 cut(s) 306
CviQI GTAC 2 cut(s) 49, 271
DdeI CTNAG 1 cut(s) 407
DinI GGCGCC 1 cut(s) 125
DpnI GATC 5 cut(s) 14, 133, 302, 317, 412
DpnII GATC 5 cut(s) 12, 131, 300, 315, 410
EaeI YGGCCR 1 cut(s) 69
EagI CGGCCG 1 cut(s) 69
EciI GGCGGA 3 cut(s) 5, 70, 335
Ecl136II GAGCTC 2 cut(s) 220, 247
EclXI CGGCCG 1 cut(s) 69
Eco24I GRGCYC 2 cut(s) 222, 249
Eco52I CGGCCG 1 cut(s) 69
Eco53kI GAGCTC 2 cut(s) 220, 247
Eco72I CACGTG 1 cut(s) 313
Eco88I CYCGRG 2 cut(s) 158, 335
EcoICRI GAGCTC 2 cut(s) 220, 247
EcoNI CCTNNNNNAGG 1 cut(s) 353
EcoO109I RGGNCCY 1 cut(s) 358
EcoT38I GRGCYC 2 cut(s) 222, 249
EgeI GGCGCC 1 cut(s) 125
EheI GGCGCC 1 cut(s) 125
FaeI CATG 1 cut(s) 309
FaiI YATR 1 cut(s) 307
FaqI GGGAC 1 cut(s) 155
FatI CATG 1 cut(s) 305
FauI CCCGC 2 cut(s) 35, 96
FblI GTMKAC 2 cut(s) 177, 402
Fnu4HI GCNGC 1 cut(s) 8
FriOI GRGCYC 2 cut(s) 222, 249
Fsp4HI GCNGC 1 cut(s) 8
GlaI GCGC 5 cut(s) 62, 92, 125, 329, 342
GluI GCNGC 1 cut(s) 8
HaeII RGCGCY 2 cut(s) 127, 344
HaeIII GGCC 4 cut(s) 71, 155, 165, 359
HapII CCGG 7 cut(s) 19, 68, 121, 127, 173, 233, 298
HgaI GACGC 2 cut(s) 19, 83
HhaI GCGC 5 cut(s) 63, 93, 126, 330, 343
Hin1I GRCGYC 2 cut(s) 75, 124
Hin1II CATG 1 cut(s) 309
Hin6I GCGC 5 cut(s) 61, 91, 124, 328, 341
HinP1I GCGC 5 cut(s) 61, 91, 124, 328, 341
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HinfI GANTC 3 cut(s) 38, 101, 291
HpaII CCGG 7 cut(s) 19, 68, 121, 127, 173, 233, 298
Hpy166II GTNNAC 2 cut(s) 178, 403
Hpy188I TCNGA 1 cut(s) 353
Hpy188III TCNNGA 2 cut(s) 223, 298
Hpy8I GTNNAC 2 cut(s) 178, 403
Hpy99I CGWCG 7 cut(s) 35, 77, 185, 191, 245, 367, 404
HpyAV CCTTC 2 cut(s) 115, 280
HpyCH4IV ACGT 3 cut(s) 168, 180, 312
HpyCH4V TGCA 1 cut(s) 376
HpyF10VI GCNNNNNNNGC 2 cut(s) 60, 340
HpyF3I CTNAG 1 cut(s) 407
HpySE526I ACGT 3 cut(s) 168, 180, 312
Hsp92I GRCGYC 2 cut(s) 75, 124
Hsp92II CATG 1 cut(s) 309
HspAI GCGC 5 cut(s) 61, 91, 124, 328, 341
KasI GGCGCC 1 cut(s) 123
Kpn2I TCCGGA 1 cut(s) 297
KroI GCCGGC 1 cut(s) 18
KroNI GCCGGC 1 cut(s) 20
Kzo9I GATC 5 cut(s) 12, 131, 300, 315, 410
LmnI GCTCC 6 cut(s) 68, 123, 196, 225, 252, 348
Lsp1109I GCAGC 1 cut(s) 19
MaeII ACGT 3 cut(s) 168, 180, 312
MalI GATC 5 cut(s) 14, 133, 302, 317, 412
MbiI CCGCTC 1 cut(s) 199
MboI GATC 5 cut(s) 12, 131, 300, 315, 410
MboII GAAGA 2 cut(s) 229, 313
MflI RGATCY 3 cut(s) 131, 300, 410
MhlI GDGCHC 3 cut(s) 222, 249, 336
Mly113I GGCGCC 1 cut(s) 124
MlyI GAGTC 3 cut(s) 32, 110, 300
MroI TCCGGA 1 cut(s) 297
MroNI GCCGGC 1 cut(s) 18
MspA1I CMGCKG 2 cut(s) 10, 140
MspI CCGG 7 cut(s) 19, 68, 121, 127, 173, 233, 298
MspR9I CCNGG 3 cut(s) 68, 173, 233
MvnI CGCG 3 cut(s) 30, 91, 330
MwoI GCNNNNNNNGC 2 cut(s) 60, 340
NaeI GCCGGC 1 cut(s) 20
NarI GGCGCC 1 cut(s) 124
NciI CCSGG 3 cut(s) 68, 173, 233
NdeII GATC 5 cut(s) 12, 131, 300, 315, 410
NgoMIV GCCGGC 1 cut(s) 18
NlaIII CATG 1 cut(s) 309
NlaIV GGNNCC 4 cut(s) 125, 156, 302, 360
NmeAIII GCCGAG 1 cut(s) 241
PaeR7I CTCGAG 1 cut(s) 335
PaqCI CACCTGC 1 cut(s) 139
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PdiI GCCGGC 1 cut(s) 20
PkrI GCNGC 1 cut(s) 9
PleI GAGTC 3 cut(s) 32, 109, 299
PluTI GGCGCC 1 cut(s) 127
PmaCI CACGTG 1 cut(s) 313
PmlI CACGTG 1 cut(s) 313
PpsI GAGTC 3 cut(s) 32, 109, 299
Ppu21I YACGTR 1 cut(s) 313
Psp124BI GAGCTC 2 cut(s) 222, 249
PspCI CACGTG 1 cut(s) 313
PspN4I GGNNCC 4 cut(s) 125, 156, 302, 360
PspPI GGNCC 3 cut(s) 154, 163, 358
PspXI VCTCGAGB 1 cut(s) 335
PsuI RGATCY 3 cut(s) 131, 300, 410
PvuII CAGCTG 1 cut(s) 10
RsaI GTAC 2 cut(s) 50, 272
RsaNI GTAC 2 cut(s) 49, 271
SacI GAGCTC 2 cut(s) 222, 249
SalI GTCGAC 1 cut(s) 401
SatI GCNGC 1 cut(s) 8
Sau3AI GATC 5 cut(s) 12, 131, 300, 315, 410
Sau96I GGNCC 3 cut(s) 154, 163, 358
ScaI AGTACT 1 cut(s) 50
SchI GAGTC 3 cut(s) 32, 110, 300
ScrFI CCNGG 3 cut(s) 68, 173, 233
SduI GDGCHC 3 cut(s) 222, 249, 336
SfoI GGCGCC 1 cut(s) 125
Sfr274I CTCGAG 1 cut(s) 335
SlaI CTCGAG 1 cut(s) 335
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
SsiI CCGC 7 cut(s) 16, 28, 81, 89, 138, 199, 346
SspDI GGCGCC 1 cut(s) 123
SstI GAGCTC 2 cut(s) 222, 249
StyD4I CCNGG 3 cut(s) 66, 171, 231
TaiI ACGT 3 cut(s) 171, 183, 315
TaqI TCGA 5 cut(s) 243, 324, 336, 392, 402
TatI WGTACW 1 cut(s) 48
TscAI CASTG 1 cut(s) 217
TseI GCWGC 1 cut(s) 7
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 98, 388
TspRI CASTG 1 cut(s) 217
XagI CCTNNNNNAGG 1 cut(s) 353
XhoI CTCGAG 1 cut(s) 335
XmiI GTMKAC 2 cut(s) 177, 402
ZrmI AGTACT 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.