RchiOBHm_Chr5g0025871

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0009941
Physical Location & Seq
Reverse (-)
19822515 .. 19823410
896 bp
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UTR
Exon/CDS
Intron
PRQ30547

Sequence Viewer

Length: 453 bp
ATGAAGCAATTGATCCGACGTCTCTCCCGCGTCGCCGACTCGTCGCAGTACTGCCTCCTCCGCAGCTCGGATTCCACCGCCGCGTCCGGTGCCTCCTCCAATTCCCGGCCTCGCCGGAGCGAGTCGTTCAGGGTCCTGAAGCTCCGGCGACGCAGGAACAACTCGGATACCGGAGTCCCCGAGGGACACGTGCCGGTGTACGTCGGCGACGAGATGGAGCGGTTCGTCGTCTCCGCCGAGCTCCTCAACCACCCGGTGTTCGTCGAGCTCCTCAACAAGTCGGCGCAGGAGTACGGCTACGAGCAGCGCGGCGTGCTCCGGATTCCCTGCCACGTGGTGGTGTTCGAGCGCGTCCTCGAGACGCTCCGGCTCGGACGCGCCGCGCCGTCGAGTGACATCAACGACCTCCTCGGCTCGCTCTCAGAGGAACGTCACTTCATCTACGCCAATTAG

Protein Analysis

150

Amino Acids

16.99

Weight (kDa)

9.38

Isoelectric Point (pI)

70.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 38 - 122 1.4e-29 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 22
AccB1I GGYRCC 1 cut(s) 89
AccB7I CCANNNNNTGG 1 cut(s) 337
AccBSI CCGCTC 1 cut(s) 220
AccII CGCG 6 cut(s) 30, 83, 309, 351, 378, 383
AccIII TCCGGA 1 cut(s) 318
AciI CCGC 8 cut(s) 28, 61, 78, 81, 220, 234, 309, 381
AclWI GGATC 1 cut(s) 7
AcuI CTGAAG 1 cut(s) 158
AcvI CACGTG 2 cut(s) 190, 334
AcyI GRCGYC 1 cut(s) 19
AdeI CACNNNGTG 2 cut(s) 256, 337
AfaI GTAC 3 cut(s) 50, 200, 293
AfiI CCNNNNNNNGG 3 cut(s) 67, 105, 337
AflIII ACRYGT 1 cut(s) 187
AluBI AGCT 4 cut(s) 66, 142, 241, 268
AluI AGCT 4 cut(s) 66, 142, 241, 268
Alw21I GWGCWC 3 cut(s) 243, 270, 318
Alw26I GTCTC 3 cut(s) 26, 235, 353
AlwI GGATC 1 cut(s) 7
Ama87I CYCGRG 2 cut(s) 179, 356
Aor13HI TCCGGA 1 cut(s) 318
AoxI GGCC 1 cut(s) 107
ApeKI GCWGC 2 cut(s) 63, 304
AspLEI GCGC 5 cut(s) 286, 309, 351, 380, 385
AspS9I GGNCC 1 cut(s) 133
AsuC2I CCSGG 2 cut(s) 106, 254
AvaI CYCGRG 2 cut(s) 179, 356
AvaII GGWCC 1 cut(s) 133
BaeI ACNNNNGTAYC 2 cut(s) 159, 192
BanI GGYRCC 1 cut(s) 89
BanII GRGCYC 2 cut(s) 243, 270
BbrPI CACGTG 2 cut(s) 190, 334
Bbv12I GWGCWC 3 cut(s) 243, 270, 318
BbvI GCAGC 2 cut(s) 75, 316
BccI CCATC 1 cut(s) 208
BceAI ACGGC 2 cut(s) 310, 370
BciVI GTATCC 1 cut(s) 160
BcnI CCSGG 2 cut(s) 106, 254
BcoDI GTCTC 3 cut(s) 26, 235, 353
BfuI GTATCC 1 cut(s) 160
BisI GCNGC 5 cut(s) 64, 81, 305, 310, 381
BlsI GCNGC 5 cut(s) 65, 82, 306, 311, 382
BmcAI AGTACT 1 cut(s) 50
Bme1390I CCNGG 2 cut(s) 106, 254
Bme18I GGWCC 1 cut(s) 133
BmeT110I CYCGRG 2 cut(s) 179, 356
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 2 cut(s) 91, 134
BmrFI CCNGG 2 cut(s) 106, 254
BpuMI CCSGG 2 cut(s) 106, 254
BsaAI YACGTR 2 cut(s) 190, 334
BsaHI GRCGYC 1 cut(s) 19
BsaJI CCNNGG 2 cut(s) 180, 409
BsaWI WCCGGW 3 cut(s) 86, 170, 318
Bsc4I CCNNNNNNNGG 3 cut(s) 67, 105, 337
Bse118I RCCGGY 1 cut(s) 193
BseAI TCCGGA 1 cut(s) 318
BseDI CCNNGG 2 cut(s) 180, 409
BseLI CCNNNNNNNGG 3 cut(s) 67, 105, 337
BseMII CTCAG 1 cut(s) 435
BseRI GAGGAG 5 cut(s) 47, 85, 233, 260, 398
BseXI GCAGC 2 cut(s) 75, 316
Bsh1236I CGCG 6 cut(s) 30, 83, 309, 351, 378, 383
BshFI GGCC 1 cut(s) 109
BshNI GGYRCC 1 cut(s) 89
BsiHKAI GWGCWC 3 cut(s) 243, 270, 318
BsiHKCI CYCGRG 2 cut(s) 179, 356
BsiSI CCGG 9 cut(s) 87, 106, 115, 145, 171, 194, 254, 319, 367
BslFI GGGAC 2 cut(s) 161, 198
BslI CCNNNNNNNGG 3 cut(s) 67, 105, 337
BsmAI GTCTC 3 cut(s) 26, 235, 353
BsmBI CGTCTC 3 cut(s) 26, 235, 353
BsmFI GGGAC 2 cut(s) 161, 198
BsnI GGCC 1 cut(s) 109
BsoBI CYCGRG 2 cut(s) 179, 356
Bsp1286I GDGCHC 3 cut(s) 243, 270, 318
Bsp13I TCCGGA 1 cut(s) 318
Bsp143I GATC 1 cut(s) 12
BspACI CCGC 8 cut(s) 28, 61, 78, 81, 220, 234, 309, 381
BspANI GGCC 1 cut(s) 109
BspCNI CTCAG 1 cut(s) 434
BspEI TCCGGA 1 cut(s) 318
BspFNI CGCG 6 cut(s) 30, 83, 309, 351, 378, 383
BspLI GGNNCC 2 cut(s) 91, 134
BspPI GGATC 1 cut(s) 7
BspT107I GGYRCC 1 cut(s) 89
BsrBI CCGCTC 1 cut(s) 220
BsrFI RCCGGY 1 cut(s) 193
BssAI RCCGGY 1 cut(s) 193
BssECI CCNNGG 2 cut(s) 180, 409
BssMI GATC 1 cut(s) 12
BssNI GRCGYC 1 cut(s) 19
BstACI GRCGYC 1 cut(s) 19
BstBAI YACGTR 2 cut(s) 190, 334
BstC8I GCNNGC 2 cut(s) 314, 416
BstDEI CTNAG 1 cut(s) 421
BstFNI CGCG 6 cut(s) 30, 83, 309, 351, 378, 383
BstHHI GCGC 5 cut(s) 286, 309, 351, 380, 385
BstKTI GATC 1 cut(s) 15
BstMAI GTCTC 3 cut(s) 26, 235, 353
BstMBI GATC 1 cut(s) 12
BstMWI GCNNNNNNNGC 3 cut(s) 60, 89, 313
BstSCI CCNGG 2 cut(s) 104, 252
BstUI CGCG 6 cut(s) 30, 83, 309, 351, 378, 383
BstV1I GCAGC 2 cut(s) 75, 316
BsuI GTATCC 1 cut(s) 160
BsuRI GGCC 1 cut(s) 109
Cac8I GCNNGC 2 cut(s) 314, 416
CfoI GCGC 5 cut(s) 286, 309, 351, 380, 385
Cfr10I RCCGGY 1 cut(s) 193
Cfr13I GGNCC 1 cut(s) 133
CseI GACGC 6 cut(s) 19, 72, 159, 340, 370, 384
Csp6I GTAC 3 cut(s) 49, 199, 292
CviJI RGCY 8 cut(s) 66, 109, 142, 241, 268, 297, 370, 414
CviKI_1 RGCY 8 cut(s) 66, 109, 142, 241, 268, 297, 370, 414
CviQI GTAC 3 cut(s) 49, 199, 292
DdeI CTNAG 1 cut(s) 421
DpnI GATC 1 cut(s) 14
DpnII GATC 1 cut(s) 12
DraIII CACNNNGTG 2 cut(s) 256, 337
EciI GGCGGA 1 cut(s) 223
Ecl136II GAGCTC 2 cut(s) 241, 268
Eco24I GRGCYC 2 cut(s) 243, 270
Eco47I GGWCC 1 cut(s) 133
Eco53kI GAGCTC 2 cut(s) 241, 268
Eco57I CTGAAG 1 cut(s) 158
Eco72I CACGTG 2 cut(s) 190, 334
Eco88I CYCGRG 2 cut(s) 179, 356
EcoICRI GAGCTC 2 cut(s) 241, 268
EcoO109I RGGNCCY 1 cut(s) 133
EcoT38I GRGCYC 2 cut(s) 243, 270
Esp3I CGTCTC 3 cut(s) 26, 235, 353
FaqI GGGAC 2 cut(s) 161, 198
FauI CCCGC 1 cut(s) 35
Fnu4HI GCNGC 5 cut(s) 64, 81, 305, 310, 381
FriOI GRGCYC 2 cut(s) 243, 270
Fsp4HI GCNGC 5 cut(s) 64, 81, 305, 310, 381
GlaI GCGC 5 cut(s) 285, 308, 350, 379, 384
GluI GCNGC 5 cut(s) 64, 81, 305, 310, 381
HaeIII GGCC 1 cut(s) 109
HapII CCGG 9 cut(s) 87, 106, 115, 145, 171, 194, 254, 319, 367
HgaI GACGC 6 cut(s) 19, 72, 159, 340, 370, 384
HhaI GCGC 5 cut(s) 286, 309, 351, 380, 385
Hin1I GRCGYC 1 cut(s) 19
Hin6I GCGC 5 cut(s) 284, 307, 349, 378, 383
HinP1I GCGC 5 cut(s) 284, 307, 349, 378, 383
HinfI GANTC 5 cut(s) 38, 71, 122, 174, 322
HpaII CCGG 9 cut(s) 87, 106, 115, 145, 171, 194, 254, 319, 367
Hpy166II GTNNAC 1 cut(s) 199
Hpy188I TCNGA 5 cut(s) 17, 70, 166, 374, 424
Hpy188III TCNNGA 3 cut(s) 136, 319, 358
Hpy8I GTNNAC 1 cut(s) 199
Hpy99I CGWCG 9 cut(s) 21, 35, 46, 153, 206, 212, 230, 266, 391
HpyCH4IV ACGT 5 cut(s) 19, 189, 201, 333, 430
HpyF10VI GCNNNNNNNGC 3 cut(s) 60, 89, 313
HpyF3I CTNAG 1 cut(s) 421
HpySE526I ACGT 5 cut(s) 19, 189, 201, 333, 430
Hsp92I GRCGYC 1 cut(s) 19
HspAI GCGC 5 cut(s) 284, 307, 349, 378, 383
Kpn2I TCCGGA 1 cut(s) 318
Kzo9I GATC 1 cut(s) 12
LmnI GCTCC 7 cut(s) 117, 147, 217, 246, 273, 321, 369
Lsp1109I GCAGC 2 cut(s) 75, 316
MaeII ACGT 5 cut(s) 19, 189, 201, 333, 430
MaeIII GTNAC 2 cut(s) 392, 431
MalI GATC 1 cut(s) 14
MbiI CCGCTC 1 cut(s) 220
MboI GATC 1 cut(s) 12
MfeI CAATTG 1 cut(s) 8
MhlI GDGCHC 3 cut(s) 243, 270, 318
MluCI AATT 3 cut(s) 8, 100, 448
MlyI GAGTC 3 cut(s) 32, 131, 183
MmeI TCCRAC 1 cut(s) 40
MroI TCCGGA 1 cut(s) 318
MspI CCGG 9 cut(s) 87, 106, 115, 145, 171, 194, 254, 319, 367
MspR9I CCNGG 2 cut(s) 106, 254
MteI GCGCNGCGC 1 cut(s) 381
MunI CAATTG 1 cut(s) 8
MvnI CGCG 6 cut(s) 30, 83, 309, 351, 378, 383
MwoI GCNNNNNNNGC 3 cut(s) 60, 89, 313
NciI CCSGG 2 cut(s) 106, 254
NdeII GATC 1 cut(s) 12
NlaIV GGNNCC 2 cut(s) 91, 134
NmeAIII GCCGAG 2 cut(s) 262, 390
NmuCI GTSAC 2 cut(s) 392, 431
PaeR7I CTCGAG 1 cut(s) 356
PcsI WCGNNNNNNNCGW 2 cut(s) 207, 234
PfeI GAWTC 2 cut(s) 71, 322
PflMI CCANNNNNTGG 1 cut(s) 337
PkrI GCNGC 5 cut(s) 65, 82, 306, 311, 382
PleI GAGTC 3 cut(s) 32, 130, 182
PmaCI CACGTG 2 cut(s) 190, 334
PmlI CACGTG 2 cut(s) 190, 334
PpsI GAGTC 3 cut(s) 32, 130, 182
Ppu21I YACGTR 2 cut(s) 190, 334
PpuMI RGGWCCY 1 cut(s) 133
Psp124BI GAGCTC 2 cut(s) 243, 270
Psp5II RGGWCCY 1 cut(s) 133
PspCI CACGTG 2 cut(s) 190, 334
PspN4I GGNNCC 2 cut(s) 91, 134
PspPI GGNCC 1 cut(s) 133
PspPPI RGGWCCY 1 cut(s) 133
RsaI GTAC 3 cut(s) 50, 200, 293
RsaNI GTAC 3 cut(s) 49, 199, 292
SacI GAGCTC 2 cut(s) 243, 270
SatI GCNGC 5 cut(s) 64, 81, 305, 310, 381
Sau3AI GATC 1 cut(s) 12
Sau96I GGNCC 1 cut(s) 133
ScaI AGTACT 1 cut(s) 50
SchI GAGTC 3 cut(s) 32, 131, 183
ScrFI CCNGG 2 cut(s) 106, 254
SduI GDGCHC 3 cut(s) 243, 270, 318
Sfr274I CTCGAG 1 cut(s) 356
SinI GGWCC 1 cut(s) 133
SlaI CTCGAG 1 cut(s) 356
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
Sse9I AATT 3 cut(s) 8, 100, 448
SsiI CCGC 8 cut(s) 28, 61, 78, 81, 220, 234, 309, 381
SstI GAGCTC 2 cut(s) 243, 270
StyD4I CCNGG 2 cut(s) 104, 252
TaiI ACGT 5 cut(s) 22, 192, 204, 336, 433
TaqI TCGA 4 cut(s) 264, 345, 357, 389
TasI AATT 3 cut(s) 8, 100, 448
TatI WGTACW 1 cut(s) 48
TauI GCSGC 3 cut(s) 83, 312, 383
TfiI GAWTC 2 cut(s) 71, 322
TseFI GTSAC 2 cut(s) 392, 431
TseI GCWGC 2 cut(s) 63, 304
Tsp45I GTSAC 2 cut(s) 392, 431
TspDTI ATGAA 2 cut(s) 17, 427
Van91I CCANNNNNTGG 1 cut(s) 337
VpaK11BI GGWCC 1 cut(s) 133
XhoI CTCGAG 1 cut(s) 356
ZraI GACGTC 1 cut(s) 20
ZrmI AGTACT 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.