Rorug01G0048500

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0009941
Physical Location & Seq
Forward (+)
7964936 .. 7980049
15114 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0048500.1

Sequence Viewer

Length: 489 bp
ATGTTTCGGAGCCTGCAAGCAGACGGACGTGCACAGGAAAGGAGAAGGATGCAGTCGGTGTGCGTTGGTGCTGCAGGGGCAGCAGGGATGCGTGCGCAGGGGCGCGTAGGTGCTGCAGGGGCAGGTGCGCAGGTGAGGCTAGCGTGGGCTAGGAGCTGCGGCAGTGAGGCTCGAAGGTGGCAATGGCAGATTAGAGTAAAGCCATTGCAACTACGTAACAGATCTTTCTCTTTGAATCAGAACTCAAAAAGGAGTCTCAAAGTTTATTCATTCATTTCTATGGCGGACGAGGAGATTGCTGATACAAAAAAGTATCTTGAGGATTCTTGTCAACCTAAATGTGTGAAGCCTTTAATTGAATATCAGGCGTGTGTCAAGAGGATTGAAGGTGATGATAGTGGTTACAAGCATTGTACTGGCCAGTACTTTGACTACATATCTTGTGTTGACAAATGTGTCGCGCCAAAGTTATTTGGAAAGCTCAAGTAA

Protein Analysis

162

Amino Acids

18.19

Weight (kDa)

9.69

Isoelectric Point (pI)

57.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UCR_hinge PF02320 101 - 162 4.8e-20 Ubiquinol-cytochrome C reductase hinge protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 113, 121
AasI GACNNNNNNGTC 1 cut(s) 455
Acc16I TGCGCA 2 cut(s) 96, 129
Acc36I ACCTGC 2 cut(s) 113, 121
AccII CGCG 2 cut(s) 105, 461
AciI CCGC 2 cut(s) 159, 284
AcoI YGGCCR 1 cut(s) 418
AfaI GTAC 2 cut(s) 415, 425
AgsI TTSAA 3 cut(s) 235, 359, 386
AjiI CACGTC 1 cut(s) 29
AluBI AGCT 2 cut(s) 156, 481
AluI AGCT 2 cut(s) 156, 481
Alw21I GWGCWC 1 cut(s) 34
Alw26I GTCTC 1 cut(s) 260
Alw44I GTGCAC 1 cut(s) 30
AoxI GGCC 1 cut(s) 418
ApaLI GTGCAC 1 cut(s) 30
ApeKI GCWGC 4 cut(s) 71, 80, 113, 156
AspLEI GCGC 4 cut(s) 97, 105, 130, 463
AsuHPI GGTGA 2 cut(s) 145, 401
AsuNHI GCTAGC 1 cut(s) 139
BaeGI GKGCMC 1 cut(s) 34
BalI TGGCCA 1 cut(s) 420
Bbv12I GWGCWC 1 cut(s) 34
BbvI GCAGC 4 cut(s) 58, 92, 100, 143
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BcoDI GTCTC 1 cut(s) 260
BfaI CTAG 2 cut(s) 140, 150
BfmI CTRYAG 2 cut(s) 72, 114
BfuAI ACCTGC 2 cut(s) 113, 121
BglII AGATCT 1 cut(s) 221
BisI GCNGC 5 cut(s) 72, 81, 114, 157, 160
BlsI GCNGC 5 cut(s) 73, 82, 115, 158, 161
BmcAI AGTACT 1 cut(s) 425
BmgBI CACGTC 1 cut(s) 29
BmiI GGNNCC 1 cut(s) 11
BmsI GCATC 2 cut(s) 39, 78
BmtI GCTAGC 1 cut(s) 143
BpuEI CTTGAG 2 cut(s) 338, 467
BsaAI YACGTR 1 cut(s) 215
Bse1I ACTGG 2 cut(s) 421, 421
Bse3DI GCAATG 2 cut(s) 188, 203
BseGI GGATG 2 cut(s) 54, 93
BseMI GCAATG 2 cut(s) 188, 203
BseNI ACTGG 2 cut(s) 421, 421
BseRI GAGGAG 1 cut(s) 305
BseSI GKGCMC 1 cut(s) 34
BseXI GCAGC 4 cut(s) 58, 92, 100, 143
Bsh1236I CGCG 2 cut(s) 105, 461
BshFI GGCC 1 cut(s) 420
BsiHKAI GWGCWC 1 cut(s) 34
BsmAI GTCTC 1 cut(s) 260
BsnI GGCC 1 cut(s) 420
Bsp1286I GDGCHC 1 cut(s) 34
Bsp143I GATC 1 cut(s) 221
BspACI CCGC 2 cut(s) 159, 284
BspANI GGCC 1 cut(s) 420
BspFNI CGCG 2 cut(s) 105, 461
BspLI GGNNCC 1 cut(s) 11
BspMAI CTGCAG 2 cut(s) 76, 118
BspMI ACCTGC 2 cut(s) 113, 121
BspOI GCTAGC 1 cut(s) 143
BsrDI GCAATG 2 cut(s) 188, 203
BsrI ACTGG 2 cut(s) 421, 421
BssMI GATC 1 cut(s) 221
BstBAI YACGTR 1 cut(s) 215
BstC8I GCNNGC 4 cut(s) 14, 18, 93, 141
BstF5I GGATG 2 cut(s) 54, 93
BstFNI CGCG 2 cut(s) 105, 461
BstHHI GCGC 4 cut(s) 97, 105, 130, 463
BstKTI GATC 1 cut(s) 224
BstMAI GTCTC 1 cut(s) 260
BstMBI GATC 1 cut(s) 221
BstMWI GCNNNNNNNGC 4 cut(s) 77, 80, 119, 136
BstSFI CTRYAG 2 cut(s) 72, 114
BstSLI GKGCMC 1 cut(s) 34
BstSNI TACGTA 1 cut(s) 215
BstUI CGCG 2 cut(s) 105, 461
BstV1I GCAGC 4 cut(s) 58, 92, 100, 143
BstX2I RGATCY 1 cut(s) 221
BstYI RGATCY 1 cut(s) 221
BsuRI GGCC 1 cut(s) 420
BtrI CACGTC 1 cut(s) 29
BtsCI GGATG 2 cut(s) 54, 93
BtsI GCAGTG 1 cut(s) 169
BtsIMutI CAGTG 1 cut(s) 169
BveI ACCTGC 2 cut(s) 113, 121
Cac8I GCNNGC 4 cut(s) 14, 18, 93, 141
CfoI GCGC 4 cut(s) 97, 105, 130, 463
Csp6I GTAC 2 cut(s) 414, 424
CviJI RGCY 9 cut(s) 12, 139, 149, 156, 170, 202, 349, 420, 481
CviKI_1 RGCY 9 cut(s) 12, 139, 149, 156, 170, 202, 349, 420, 481
CviQI GTAC 2 cut(s) 414, 424
DpnI GATC 1 cut(s) 223
DpnII GATC 1 cut(s) 221
DrdI GACNNNNNNGTC 1 cut(s) 455
DseDI GACNNNNNNGTC 1 cut(s) 455
EaeI YGGCCR 1 cut(s) 418
EciI GGCGGA 1 cut(s) 299
Eco105I TACGTA 1 cut(s) 215
FaiI YATR 2 cut(s) 281, 437
Fnu4HI GCNGC 5 cut(s) 72, 81, 114, 157, 160
FokI GGATG 2 cut(s) 61, 100
Fsp4HI GCNGC 5 cut(s) 72, 81, 114, 157, 160
FspBI CTAG 2 cut(s) 140, 150
FspI TGCGCA 2 cut(s) 96, 129
GlaI GCGC 4 cut(s) 96, 104, 129, 462
GluI GCNGC 5 cut(s) 72, 81, 114, 157, 160
HaeIII GGCC 1 cut(s) 420
HhaI GCGC 4 cut(s) 97, 105, 130, 463
Hin6I GCGC 4 cut(s) 95, 103, 128, 461
HinP1I GCGC 4 cut(s) 95, 103, 128, 461
HincII GTYRAC 2 cut(s) 332, 448
HindII GTYRAC 2 cut(s) 332, 448
HinfI GANTC 3 cut(s) 235, 253, 323
HphI GGTGA 2 cut(s) 145, 401
Hpy166II GTNNAC 3 cut(s) 32, 332, 448
Hpy188I TCNGA 2 cut(s) 9, 240
Hpy188III TCNNGA 2 cut(s) 317, 376
Hpy8I GTNNAC 3 cut(s) 32, 332, 448
HpyAV CCTTC 3 cut(s) 39, 168, 380
HpyCH4IV ACGT 2 cut(s) 28, 214
HpyCH4V TGCA 6 cut(s) 16, 32, 52, 74, 116, 208
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 80, 119, 136
HpySE526I ACGT 2 cut(s) 28, 214
HspAI GCGC 4 cut(s) 95, 103, 128, 461
Kzo9I GATC 1 cut(s) 221
LmnI GCTCC 2 cut(s) 9, 153
Lsp1109I GCAGC 4 cut(s) 58, 92, 100, 143
LweI GCATC 2 cut(s) 39, 78
MaeI CTAG 2 cut(s) 140, 150
MaeII ACGT 2 cut(s) 28, 214
MaeIII GTNAC 2 cut(s) 215, 401
MalI GATC 1 cut(s) 223
MboI GATC 1 cut(s) 221
MflI RGATCY 1 cut(s) 221
MhlI GDGCHC 1 cut(s) 34
MlsI TGGCCA 1 cut(s) 420
MluCI AATT 1 cut(s) 354
MluNI TGGCCA 1 cut(s) 420
MlyI GAGTC 1 cut(s) 262
MnlI CCTC 5 cut(s) 129, 160, 283, 313, 372
Mox20I TGGCCA 1 cut(s) 420
MscI TGGCCA 1 cut(s) 420
MseI TTAA 1 cut(s) 353
MslI CAYNNNNRTG 1 cut(s) 278
Msp20I TGGCCA 1 cut(s) 420
MvnI CGCG 2 cut(s) 105, 461
MwoI GCNNNNNNNGC 4 cut(s) 77, 80, 119, 136
NdeII GATC 1 cut(s) 221
NheI GCTAGC 1 cut(s) 139
NlaIV GGNNCC 1 cut(s) 11
NsbI TGCGCA 2 cut(s) 96, 129
PaqCI CACCTGC 2 cut(s) 113, 121
PfeI GAWTC 2 cut(s) 235, 323
PkrI GCNGC 5 cut(s) 73, 82, 115, 158, 161
PleI GAGTC 1 cut(s) 261
PpsI GAGTC 1 cut(s) 261
Ppu21I YACGTR 1 cut(s) 215
PspN4I GGNNCC 1 cut(s) 11
PstI CTGCAG 2 cut(s) 76, 118
PsuI RGATCY 1 cut(s) 221
RsaI GTAC 2 cut(s) 415, 425
RsaNI GTAC 2 cut(s) 414, 424
RseI CAYNNNNRTG 1 cut(s) 278
SaqAI TTAA 1 cut(s) 353
SatI GCNGC 5 cut(s) 72, 81, 114, 157, 160
Sau3AI GATC 1 cut(s) 221
ScaI AGTACT 1 cut(s) 425
SchI GAGTC 1 cut(s) 262
SduI GDGCHC 1 cut(s) 34
SfaNI GCATC 2 cut(s) 39, 78
SfcI CTRYAG 2 cut(s) 72, 114
SmiMI CAYNNNNRTG 1 cut(s) 278
SmlI CTYRAG 2 cut(s) 317, 482
SmoI CTYRAG 2 cut(s) 317, 482
SnaBI TACGTA 1 cut(s) 215
Sse9I AATT 1 cut(s) 354
SsiI CCGC 2 cut(s) 159, 284
SspMI CTAG 2 cut(s) 140, 150
TaiI ACGT 2 cut(s) 31, 217
TaqI TCGA 1 cut(s) 172
TasI AATT 1 cut(s) 354
TatI WGTACW 2 cut(s) 413, 423
TauI GCSGC 1 cut(s) 162
TfiI GAWTC 2 cut(s) 235, 323
Tru1I TTAA 1 cut(s) 353
Tru9I TTAA 1 cut(s) 353
TscAI CASTG 1 cut(s) 169
TseI GCWGC 4 cut(s) 71, 80, 113, 156
TspDTI ATGAA 2 cut(s) 258, 262
TspGWI ACGGA 1 cut(s) 39
TspRI CASTG 1 cut(s) 169
VneI GTGCAC 1 cut(s) 30
XspI CTAG 2 cut(s) 140, 150
ZrmI AGTACT 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.