Rorug05G0089100

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0009941
Physical Location & Seq
Reverse (-)
7640232 .. 7641296
1065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0089100.1

Sequence Viewer

Length: 1065 bp
ATGGTGTCCAAAATAATGAAGATTAGAGCTAGCAAGTTTTCATCCATCAAGAACCGCGACGGCATAAGGCGGCACCACCGCCGCAACTCCTCAGTAAAGAAGGCAGCCGCCGACTCCAAGGTCGTGCTTGCCCGCACTGGCAGCCACAAATGCAGCTTCGAGATCCTAACCCGAACTCAAACTCCAGACCCGGCCGGCCCCGACCCCGGACCTCAAACTACACGCCCCCCACTCCCGCCGTTAACTTCGCCGGAGAAGAGAACCGTGTTTCTCGACCTGGACGAAACACTGGTGCACACTGAGGCCACAGTGCCCCAGCGCTTCAACTTCGTCGTCCGGCCCATTATTCGTGGCACGAAGATGACCTTCTACGTGATCAAACGTCCCGGCGTTGACCAGTTTTTGCAAAGACTGGCAGAGAAGTACGAGGTGGTGGTGTTCACGGCGGGGATGAGAGAGTACGGCACGCTGGTGGTGGACCGACTGGACAGGCAGGGCGTGATATCCCACAGCCTCTACCGCGACTCATGCAAGGAGATGAACGGAAGGTTTGTGAAAGACCTGTCCGGTTTGGGACGTGACTTGCGGCGAGTGGTGATCGTGGACGATAATCCCAACTCGTATTTCCTCCAACCGGAAAATGGGGTTCCGGTCCGGCCGTTCGTCAATGACATGGCAGACGGGGAGCTGGGGAGGTTGTTGGAGTTTTTTGAGGGAGAGGGGTCACGTTGTTCTGAGGACATTAGAGATGCTGTGAGGGAGTATGTTCATGACAAAGGATGGGTTTCCGATGATAATGACGACGATGAGAATGATGAAGATGATGATAATGGTGGTAGTGATAATGCTGATGATGATGAGTTGGAGACGGGGAATGGAAAGTCTACTAGTGTTGCAGTATCAACAACCATTAATTCATCAACACCACGCTGCTTGATTAGTCTGCATGAATATTCTCCATCGGGAAAACCAGTAGCTATGGAGAGTTGGATGAAGATGGTGATCTTAGGCAGTTTGATATTGATCATTATCGATGTAATTTTTTGCTTTCGGGCATTTTTGTAA

Protein Analysis

354

Amino Acids

39.73

Weight (kDa)

5.75

Isoelectric Point (pI)

36.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 88 - 249 1.4e-51 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AccB1I GGYRCC 1 cut(s) 72
AccI GTMKAC 1 cut(s) 884
AccII CGCG 2 cut(s) 57, 522
AclWI GGATC 1 cut(s) 157
AcoI YGGCCR 2 cut(s) 192, 656
AfaI GTAC 2 cut(s) 425, 461
AfeI AGCGCT 1 cut(s) 320
AfiI CCNNNNNNNGG 3 cut(s) 206, 634, 641
AgsI TTSAA 1 cut(s) 325
AhlI ACTAGT 1 cut(s) 887
AjiI CACGTC 1 cut(s) 578
AjnI CCWGG 1 cut(s) 276
AjuI GAANNNNNNNTTGG 2 cut(s) 608, 640
AleI CACNNNNGTG 1 cut(s) 470
AloI GAACNNNNNNTCC 2 cut(s) 166, 198
AluBI AGCT 4 cut(s) 29, 156, 688, 977
AluI AGCT 4 cut(s) 29, 156, 688, 977
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 1 cut(s) 860
Alw44I GTGCAC 1 cut(s) 293
AlwI GGATC 1 cut(s) 157
Aor51HI AGCGCT 1 cut(s) 320
AoxI GGCC 5 cut(s) 192, 196, 303, 338, 656
ApaLI GTGCAC 1 cut(s) 293
ApeKI GCWGC 4 cut(s) 104, 141, 153, 930
AseI ATTAAT 1 cut(s) 912
AspLEI GCGC 1 cut(s) 321
AspS9I GGNCC 5 cut(s) 197, 209, 339, 478, 652
AsuC2I CCSGG 3 cut(s) 191, 207, 387
AsuHPI GGTGA 2 cut(s) 607, 1012
AsuNHI GCTAGC 1 cut(s) 29
AvaII GGWCC 3 cut(s) 209, 478, 652
BaeGI GKGCMC 2 cut(s) 297, 315
BanI GGYRCC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 4 cut(s) 116, 153, 165, 917
BccI CCATC 4 cut(s) 53, 774, 967, 991
BceAI ACGGC 5 cut(s) 76, 223, 459, 478, 643
BciT130I CCWGG 1 cut(s) 278
BclI TGATCA 2 cut(s) 375, 1023
BcnI CCSGG 3 cut(s) 191, 207, 387
BcoDI GTCTC 1 cut(s) 860
BcuI ACTAGT 1 cut(s) 887
BfaI CTAG 2 cut(s) 30, 888
BfoI RGCGCY 1 cut(s) 322
BisI GCNGC 8 cut(s) 71, 82, 105, 108, 142, 154, 587, 931
BlsI GCNGC 8 cut(s) 72, 83, 106, 109, 143, 155, 588, 932
Bme1390I CCNGG 4 cut(s) 191, 207, 278, 387
Bme18I GGWCC 3 cut(s) 209, 478, 652
BmgBI CACGTC 1 cut(s) 578
BmgT120I GGNCC 5 cut(s) 197, 209, 339, 478, 652
BmiI GGNNCC 3 cut(s) 74, 199, 648
BmrFI CCNGG 4 cut(s) 191, 207, 278, 387
BmsI GCATC 1 cut(s) 739
BmtI GCTAGC 1 cut(s) 33
BpmI CTGGAG 1 cut(s) 168
BpuMI CCSGG 3 cut(s) 191, 207, 387
Bsa29I ATCGAT 1 cut(s) 1032
BsaAI YACGTR 1 cut(s) 373
BsaBI GATNNNNATC 3 cut(s) 1001, 1022, 1028
BsaJI CCNNGG 2 cut(s) 117, 205
BsaWI WCCGGW 3 cut(s) 566, 634, 649
BsaXI ACNNNNNCTCC 2 cut(s) 166, 196
Bsc4I CCNNNNNNNGG 3 cut(s) 206, 634, 641
Bse118I RCCGGY 1 cut(s) 194
Bse1I ACTGG 6 cut(s) 142, 294, 397, 417, 489, 971
Bse8I GATNNNNATC 3 cut(s) 1001, 1022, 1028
BseBI CCWGG 1 cut(s) 278
BseCI ATCGAT 1 cut(s) 1032
BseDI CCNNGG 2 cut(s) 117, 205
BseGI GGATG 4 cut(s) 41, 456, 785, 996
BseJI GATNNNNATC 3 cut(s) 1001, 1022, 1028
BseLI CCNNNNNNNGG 3 cut(s) 206, 634, 641
BseMII CTCAG 3 cut(s) 105, 291, 726
BseNI ACTGG 6 cut(s) 142, 294, 397, 417, 489, 971
BseRI GAGGAG 1 cut(s) 79
BseSI GKGCMC 2 cut(s) 297, 315
BseX3I CGGCCG 2 cut(s) 192, 656
BseXI GCAGC 4 cut(s) 116, 153, 165, 917
BseYI CCCAGC 2 cut(s) 315, 688
Bsh1236I CGCG 2 cut(s) 57, 522
Bsh1285I CGRYCG 2 cut(s) 195, 659
BshFI GGCC 5 cut(s) 194, 198, 305, 340, 658
BshNI GGYRCC 1 cut(s) 72
BshVI ATCGAT 1 cut(s) 1032
BsiEI CGRYCG 2 cut(s) 195, 659
BsiHKAI GWGCWC 1 cut(s) 297
BslFI GGGAC 2 cut(s) 369, 588
BslI CCNNNNNNNGG 3 cut(s) 206, 634, 641
BsmAI GTCTC 1 cut(s) 860
BsmBI CGTCTC 1 cut(s) 860
BsmFI GGGAC 2 cut(s) 369, 588
BsnI GGCC 5 cut(s) 194, 198, 305, 340, 658
Bsp1286I GDGCHC 2 cut(s) 297, 315
Bsp143I GATC 5 cut(s) 162, 375, 597, 1002, 1023
BspANI GGCC 5 cut(s) 194, 198, 305, 340, 658
BspCNI CTCAG 3 cut(s) 104, 292, 727
BspDI ATCGAT 1 cut(s) 1032
BspFNI CGCG 2 cut(s) 57, 522
BspHI TCATGA 1 cut(s) 769
BspLI GGNNCC 3 cut(s) 74, 199, 648
BspOI GCTAGC 1 cut(s) 33
BspPI GGATC 1 cut(s) 157
BspT107I GGYRCC 1 cut(s) 72
BsrFI RCCGGY 1 cut(s) 194
BsrI ACTGG 6 cut(s) 142, 294, 397, 417, 489, 971
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 2 cut(s) 117, 205
BssMI GATC 5 cut(s) 162, 375, 597, 1002, 1023
BssT1I CCWWGG 1 cut(s) 117
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 2 cut(s) 265, 310
Bst6I CTCTTC 1 cut(s) 251
BstBAI YACGTR 1 cut(s) 373
BstC8I GCNNGC 5 cut(s) 31, 129, 133, 196, 467
BstDEI CTNAG 4 cut(s) 91, 300, 735, 1006
BstF5I GGATG 4 cut(s) 41, 456, 785, 996
BstFNI CGCG 2 cut(s) 57, 522
BstH2I RGCGCY 1 cut(s) 322
BstHHI GCGC 1 cut(s) 321
BstKTI GATC 5 cut(s) 165, 378, 600, 1005, 1026
BstMAI GTCTC 1 cut(s) 860
BstMBI GATC 5 cut(s) 162, 375, 597, 1002, 1023
BstMCI CGRYCG 2 cut(s) 195, 659
BstMWI GCNNNNNNNGC 4 cut(s) 141, 150, 519, 528
BstNI CCWGG 1 cut(s) 278
BstSCI CCNGG 4 cut(s) 189, 205, 276, 385
BstSLI GKGCMC 2 cut(s) 297, 315
BstUI CGCG 2 cut(s) 57, 522
BstV1I GCAGC 4 cut(s) 116, 153, 165, 917
BstX2I RGATCY 1 cut(s) 162
BstYI RGATCY 1 cut(s) 162
BstZI CGGCCG 2 cut(s) 192, 656
Bsu15I ATCGAT 1 cut(s) 1032
BsuRI GGCC 5 cut(s) 194, 198, 305, 340, 658
BsuTUI ATCGAT 1 cut(s) 1032
BtrI CACGTC 1 cut(s) 578
BtsCI GGATG 4 cut(s) 41, 456, 785, 996
BtsIMutI CAGTG 4 cut(s) 135, 287, 297, 315
Cac8I GCNNGC 5 cut(s) 31, 129, 133, 196, 467
CciI TCATGA 1 cut(s) 769
CfoI GCGC 1 cut(s) 321
Cfr10I RCCGGY 1 cut(s) 194
Cfr13I GGNCC 5 cut(s) 197, 209, 339, 478, 652
ClaI ATCGAT 1 cut(s) 1032
CpoI CGGWCCG 1 cut(s) 652
Csp6I GTAC 2 cut(s) 424, 460
CspCI CAANNNNNGTGG 2 cut(s) 915, 950
CspI CGGWCCG 1 cut(s) 652
CviAII CATG 4 cut(s) 528, 673, 770, 947
CviQI GTAC 2 cut(s) 424, 460
DdeI CTNAG 4 cut(s) 91, 300, 735, 1006
DpnI GATC 5 cut(s) 164, 377, 599, 1004, 1025
DpnII GATC 5 cut(s) 162, 375, 597, 1002, 1023
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 2 cut(s) 192, 656
EagI CGGCCG 2 cut(s) 192, 656
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
EclXI CGGCCG 2 cut(s) 192, 656
Eco130I CCWWGG 1 cut(s) 117
Eco32I GATATC 1 cut(s) 504
Eco47I GGWCC 3 cut(s) 209, 478, 652
Eco47III AGCGCT 1 cut(s) 320
Eco52I CGGCCG 2 cut(s) 192, 656
EcoRII CCWGG 1 cut(s) 276
EcoRV GATATC 1 cut(s) 504
EcoT14I CCWWGG 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 117
Esp3I CGTCTC 1 cut(s) 860
FaeI CATG 4 cut(s) 531, 676, 773, 950
FaiI YATR 7 cut(s) 65, 529, 674, 765, 771, 948, 980
FalI AAGNNNNNCTT 2 cut(s) 350, 382
FaqI GGGAC 2 cut(s) 369, 588
FatI CATG 4 cut(s) 527, 672, 769, 946
FauI CCCGC 3 cut(s) 140, 243, 439
FbaI TGATCA 2 cut(s) 375, 1023
FblI GTMKAC 1 cut(s) 884
Fnu4HI GCNGC 8 cut(s) 71, 82, 105, 108, 142, 154, 587, 931
FokI GGATG 4 cut(s) 28, 463, 792, 1003
FseI GGCCGGCC 1 cut(s) 198
Fsp4HI GCNGC 8 cut(s) 71, 82, 105, 108, 142, 154, 587, 931
FspBI CTAG 2 cut(s) 30, 888
GlaI GCGC 1 cut(s) 320
GluI GCNGC 8 cut(s) 71, 82, 105, 108, 142, 154, 587, 931
GsaI CCCAGC 2 cut(s) 319, 692
GsuI CTGGAG 1 cut(s) 168
HaeII RGCGCY 1 cut(s) 322
HaeIII GGCC 5 cut(s) 194, 198, 305, 340, 658
HhaI GCGC 1 cut(s) 321
Hin1II CATG 4 cut(s) 531, 676, 773, 950
Hin6I GCGC 1 cut(s) 319
HinP1I GCGC 1 cut(s) 319
HincII GTYRAC 2 cut(s) 243, 394
HindII GTYRAC 2 cut(s) 243, 394
HinfI GANTC 2 cut(s) 113, 524
HpaI GTTAAC 1 cut(s) 243
HphI GGTGA 2 cut(s) 607, 1012
Hpy166II GTNNAC 7 cut(s) 243, 295, 394, 441, 478, 604, 885
Hpy188I TCNGA 2 cut(s) 736, 790
Hpy188III TCNNGA 6 cut(s) 49, 160, 185, 272, 770, 963
Hpy8I GTNNAC 7 cut(s) 243, 295, 394, 441, 478, 604, 885
Hpy99I CGWCG 3 cut(s) 62, 335, 806
HpyAV CCTTC 3 cut(s) 94, 376, 540
HpyCH4III ACNGT 2 cut(s) 265, 310
HpyCH4IV ACGT 4 cut(s) 372, 382, 577, 727
HpyCH4V TGCA 6 cut(s) 153, 295, 406, 531, 896, 946
HpyF10VI GCNNNNNNNGC 4 cut(s) 141, 150, 519, 528
HpyF3I CTNAG 4 cut(s) 91, 300, 735, 1006
HpySE526I ACGT 4 cut(s) 372, 382, 577, 727
Hsp92II CATG 4 cut(s) 531, 676, 773, 950
HspAI GCGC 1 cut(s) 319
KroI GCCGGC 1 cut(s) 194
KroNI GCCGGC 1 cut(s) 196
Ksp22I TGATCA 2 cut(s) 375, 1023
KspAI GTTAAC 1 cut(s) 243
Kzo9I GATC 5 cut(s) 162, 375, 597, 1002, 1023
LmnI GCTCC 1 cut(s) 685
Lsp1109I GCAGC 4 cut(s) 116, 153, 165, 917
LweI GCATC 1 cut(s) 739
MaeI CTAG 2 cut(s) 30, 888
MaeII ACGT 4 cut(s) 372, 382, 577, 727
MaeIII GTNAC 2 cut(s) 578, 723
MalI GATC 5 cut(s) 164, 377, 599, 1004, 1025
MboI GATC 5 cut(s) 162, 375, 597, 1002, 1023
MboII GAAGA 5 cut(s) 31, 268, 370, 830, 1006
MflI RGATCY 1 cut(s) 162
MhlI GDGCHC 2 cut(s) 297, 315
MluCI AATT 2 cut(s) 913, 1038
MlyI GAGTC 2 cut(s) 107, 518
MmeI TCCRAC 4 cut(s) 655, 681, 843, 968
MroNI GCCGGC 1 cut(s) 194
MseI TTAA 2 cut(s) 242, 912
MslI CAYNNNNRTG 2 cut(s) 359, 470
MspR9I CCNGG 4 cut(s) 191, 207, 278, 387
MvaI CCWGG 1 cut(s) 278
MvnI CGCG 2 cut(s) 57, 522
MwoI GCNNNNNNNGC 4 cut(s) 141, 150, 519, 528
NaeI GCCGGC 1 cut(s) 196
NciI CCSGG 3 cut(s) 191, 207, 387
NdeII GATC 5 cut(s) 162, 375, 597, 1002, 1023
NgoMIV GCCGGC 1 cut(s) 194
NheI GCTAGC 1 cut(s) 29
NlaIII CATG 4 cut(s) 531, 676, 773, 950
NlaIV GGNNCC 3 cut(s) 74, 199, 648
NmuCI GTSAC 2 cut(s) 578, 723
OliI CACNNNNGTG 1 cut(s) 470
PagI TCATGA 1 cut(s) 769
PcsI WCGNNNNNNNCGW 1 cut(s) 279
PdiI GCCGGC 1 cut(s) 196
PkrI GCNGC 8 cut(s) 72, 83, 106, 109, 143, 155, 588, 932
PleI GAGTC 2 cut(s) 107, 518
PpsI GAGTC 2 cut(s) 107, 518
Ppu21I YACGTR 1 cut(s) 373
PshBI ATTAAT 1 cut(s) 912
Psp6I CCWGG 1 cut(s) 276
PspFI CCCAGC 2 cut(s) 315, 688
PspGI CCWGG 1 cut(s) 276
PspN4I GGNNCC 3 cut(s) 74, 199, 648
PspPI GGNCC 5 cut(s) 197, 209, 339, 478, 652
PsuI RGATCY 1 cut(s) 162
RigI GGCCGGCC 1 cut(s) 198
RsaI GTAC 2 cut(s) 425, 461
RsaNI GTAC 2 cut(s) 424, 460
RseI CAYNNNNRTG 2 cut(s) 359, 470
Rsr2I CGGWCCG 1 cut(s) 652
RsrII CGGWCCG 1 cut(s) 652
SaqAI TTAA 2 cut(s) 242, 912
SatI GCNGC 8 cut(s) 71, 82, 105, 108, 142, 154, 587, 931
Sau3AI GATC 5 cut(s) 162, 375, 597, 1002, 1023
Sau96I GGNCC 5 cut(s) 197, 209, 339, 478, 652
SchI GAGTC 2 cut(s) 107, 518
ScrFI CCNGG 4 cut(s) 191, 207, 278, 387
SduI GDGCHC 2 cut(s) 297, 315
SfaNI GCATC 1 cut(s) 739
SinI GGWCC 3 cut(s) 209, 478, 652
SmiMI CAYNNNNRTG 2 cut(s) 359, 470
SpeI ACTAGT 1 cut(s) 887
Sse9I AATT 2 cut(s) 913, 1038
SspI AATATT 1 cut(s) 953
SspMI CTAG 2 cut(s) 30, 888
StyD4I CCNGG 4 cut(s) 189, 205, 276, 385
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 2 cut(s) 265, 310
TaiI ACGT 4 cut(s) 375, 385, 580, 730
TaqI TCGA 3 cut(s) 159, 273, 1032
TaqII GACCGA 1 cut(s) 495
TasI AATT 2 cut(s) 913, 1038
TauI GCSGC 4 cut(s) 73, 84, 110, 589
Tru1I TTAA 2 cut(s) 242, 912
Tru9I TTAA 2 cut(s) 242, 912
TscAI CASTG 4 cut(s) 142, 294, 304, 315
TseFI GTSAC 2 cut(s) 578, 723
TseI GCWGC 4 cut(s) 104, 141, 153, 930
Tsp45I GTSAC 2 cut(s) 578, 723
TspDTI ATGAA 8 cut(s) 30, 32, 554, 758, 831, 906, 963, 1007
TspGWI ACGGA 1 cut(s) 558
TspRI CASTG 4 cut(s) 142, 294, 304, 315
VneI GTGCAC 1 cut(s) 293
VpaK11BI GGWCC 3 cut(s) 209, 478, 652
VspI ATTAAT 1 cut(s) 912
XcmI CCANNNNNNNNNTGG 1 cut(s) 638
XmiI GTMKAC 1 cut(s) 884
XspI CTAG 2 cut(s) 30, 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.