RLG00000017123

Auxin-induced protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009941
Physical Location & Seq
Reverse (-)
13008248 .. 13008697
450 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017123

Sequence Viewer

Length: 375 bp
ATGGAACGGTTTGTCGTCTCCGCCGAGCTCCTCAACCACCTGGTGTTCATTGAGCTCCTCAACAAGTCAGCGCAAGAGTACGGCAACGAGCAGCACGGCGTGCTTCGGATTCTCTGTCGGGCGGTGGTGTTCGAGCGCGTCCTCGAGACGCTCTGGCTGGGGTGGGTTTCATCTTTCTCTCTCCGCATGTTATCTCTGGAGTCCAAAATGGGAAGACCTCATAAATTTTCGATAAGTGGTTATCCAAAATGGAAGACGACAGGCGGCTATGCGAGTCTTGAAGAACAGAAGGGTTTGTCTTCTATTGATGCTGGTGTTATTAAGAGTCTGCTATTAATTGTGGCGGTGGTGAAAACAAGGTGGGTGGGAGGATAG

Protein Analysis

125

Amino Acids

13.94

Weight (kDa)

9.41

Isoelectric Point (pI)

42.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 1 - 51 3.5e-14 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 138
AciI CCGC 5 cut(s) 21, 122, 184, 264, 344
AcsI RAATTY 1 cut(s) 224
AdeI CACNNNGTG 2 cut(s) 43, 100
AfaI GTAC 1 cut(s) 80
AgsI TTSAA 1 cut(s) 281
AjnI CCWGG 1 cut(s) 39
AluBI AGCT 2 cut(s) 28, 55
AluI AGCT 2 cut(s) 28, 55
Alw21I GWGCWC 2 cut(s) 30, 57
Alw26I GTCTC 2 cut(s) 22, 140
Ama87I CYCGRG 1 cut(s) 143
ApeKI GCWGC 1 cut(s) 91
ApoI RAATTY 1 cut(s) 224
AseI ATTAAT 1 cut(s) 335
AspLEI GCGC 2 cut(s) 73, 138
AsuHPI GGTGA 1 cut(s) 361
AvaI CYCGRG 1 cut(s) 143
BanII GRGCYC 2 cut(s) 30, 57
BbsI GAAGAC 3 cut(s) 220, 260, 291
Bbv12I GWGCWC 2 cut(s) 30, 57
BbvI GCAGC 1 cut(s) 103
BceAI ACGGC 2 cut(s) 97, 112
BciT130I CCWGG 1 cut(s) 41
BcoDI GTCTC 2 cut(s) 22, 140
BisI GCNGC 2 cut(s) 92, 265
BlsI GCNGC 2 cut(s) 93, 266
Bme1390I CCNGG 1 cut(s) 41
BmeT110I CYCGRG 1 cut(s) 143
BmrFI CCNGG 1 cut(s) 41
BmsI GCATC 1 cut(s) 298
BpiI GAAGAC 3 cut(s) 220, 260, 291
BpmI CTGGAG 1 cut(s) 218
BseBI CCWGG 1 cut(s) 41
BseRI GAGGAG 2 cut(s) 20, 47
BseXI GCAGC 1 cut(s) 103
BseYI CCCAGC 1 cut(s) 157
Bsh1236I CGCG 1 cut(s) 138
BsiHKAI GWGCWC 2 cut(s) 30, 57
BsiHKCI CYCGRG 1 cut(s) 143
BsmAI GTCTC 2 cut(s) 22, 140
BsmBI CGTCTC 2 cut(s) 22, 140
BsoBI CYCGRG 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 30, 57
BspACI CCGC 5 cut(s) 21, 122, 184, 264, 344
BspFNI CGCG 1 cut(s) 138
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 1 cut(s) 9
BstAPI GCANNNNNTGC 1 cut(s) 100
BstC8I GCNNGC 1 cut(s) 101
BstFNI CGCG 1 cut(s) 138
BstHHI GCGC 2 cut(s) 73, 138
BstMAI GTCTC 2 cut(s) 22, 140
BstMWI GCNNNNNNNGC 1 cut(s) 100
BstNI CCWGG 1 cut(s) 41
BstNSI RCATGY 1 cut(s) 190
BstSCI CCNGG 1 cut(s) 39
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 1 cut(s) 103
BstV2I GAAGAC 3 cut(s) 220, 260, 291
Cac8I GCNNGC 1 cut(s) 101
CfoI GCGC 2 cut(s) 73, 138
CseI GACGC 2 cut(s) 127, 157
CsiI ACCWGGT 1 cut(s) 39
Csp6I GTAC 1 cut(s) 79
CspCI CAANNNNNGTGG 1 cut(s) 345
CviAII CATG 1 cut(s) 187
CviJI RGCY 4 cut(s) 28, 55, 157, 267
CviKI_1 RGCY 4 cut(s) 28, 55, 157, 267
CviQI GTAC 1 cut(s) 79
DraIII CACNNNGTG 2 cut(s) 43, 100
EciI GGCGGA 1 cut(s) 10
Ecl136II GAGCTC 2 cut(s) 28, 55
Eco24I GRGCYC 2 cut(s) 30, 57
Eco53kI GAGCTC 2 cut(s) 28, 55
Eco88I CYCGRG 1 cut(s) 143
EcoICRI GAGCTC 2 cut(s) 28, 55
EcoRII CCWGG 1 cut(s) 39
EcoT38I GRGCYC 2 cut(s) 30, 57
Esp3I CGTCTC 2 cut(s) 22, 140
FaeI CATG 1 cut(s) 190
FaiI YATR 3 cut(s) 188, 222, 270
FatI CATG 1 cut(s) 186
Fnu4HI GCNGC 2 cut(s) 92, 265
FriOI GRGCYC 2 cut(s) 30, 57
Fsp4HI GCNGC 2 cut(s) 92, 265
GlaI GCGC 2 cut(s) 72, 137
GluI GCNGC 2 cut(s) 92, 265
GsaI CCCAGC 1 cut(s) 161
GsuI CTGGAG 1 cut(s) 218
HgaI GACGC 2 cut(s) 127, 157
HhaI GCGC 2 cut(s) 73, 138
Hin1II CATG 1 cut(s) 190
Hin6I GCGC 2 cut(s) 71, 136
HinP1I GCGC 2 cut(s) 71, 136
HinfI GANTC 4 cut(s) 109, 200, 274, 325
HphI GGTGA 1 cut(s) 361
Hpy188I TCNGA 1 cut(s) 108
Hpy188III TCNNGA 3 cut(s) 145, 197, 278
HpyAV CCTTC 1 cut(s) 283
HpyCH4III ACNGT 1 cut(s) 9
HpyF10VI GCNNNNNNNGC 1 cut(s) 100
Hsp92II CATG 1 cut(s) 190
HspAI GCGC 2 cut(s) 71, 136
LmnI GCTCC 2 cut(s) 33, 60
LpnPI CCDG 7 cut(s) 26, 53, 139, 143, 182, 246, 297
Lsp1109I GCAGC 1 cut(s) 103
LweI GCATC 1 cut(s) 298
MabI ACCWGGT 1 cut(s) 39
MboII GAAGA 4 cut(s) 225, 265, 291, 293
MhlI GDGCHC 2 cut(s) 30, 57
MluCI AATT 2 cut(s) 224, 336
MlyI GAGTC 3 cut(s) 209, 283, 334
MnlI CCTC 5 cut(s) 41, 68, 152, 228, 362
MseI TTAA 2 cut(s) 321, 335
MspR9I CCNGG 1 cut(s) 41
MvaI CCWGG 1 cut(s) 41
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 1 cut(s) 100
NlaIII CATG 1 cut(s) 190
NmeAIII GCCGAG 1 cut(s) 49
NspI RCATGY 1 cut(s) 190
PaeR7I CTCGAG 1 cut(s) 143
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PfeI GAWTC 1 cut(s) 109
PkrI GCNGC 2 cut(s) 93, 266
PleI GAGTC 3 cut(s) 208, 282, 333
PpsI GAGTC 3 cut(s) 208, 282, 333
PshBI ATTAAT 1 cut(s) 335
Psp124BI GAGCTC 2 cut(s) 30, 57
Psp6I CCWGG 1 cut(s) 39
PspFI CCCAGC 1 cut(s) 157
PspGI CCWGG 1 cut(s) 39
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
SacI GAGCTC 2 cut(s) 30, 57
SaqAI TTAA 2 cut(s) 321, 335
SatI GCNGC 2 cut(s) 92, 265
SchI GAGTC 3 cut(s) 209, 283, 334
ScrFI CCNGG 1 cut(s) 41
SduI GDGCHC 2 cut(s) 30, 57
SetI ASST 5 cut(s) 30, 42, 57, 220, 362
SexAI ACCWGGT 1 cut(s) 39
SfaNI GCATC 1 cut(s) 298
Sfr274I CTCGAG 1 cut(s) 143
SlaI CTCGAG 1 cut(s) 143
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
Sse9I AATT 2 cut(s) 224, 336
SsiI CCGC 5 cut(s) 21, 122, 184, 264, 344
SstI GAGCTC 2 cut(s) 30, 57
StyD4I CCNGG 1 cut(s) 39
TaaI ACNGT 1 cut(s) 9
TaqI TCGA 3 cut(s) 132, 144, 230
TasI AATT 2 cut(s) 224, 336
TauI GCSGC 1 cut(s) 267
TfiI GAWTC 1 cut(s) 109
Tru1I TTAA 2 cut(s) 321, 335
Tru9I TTAA 2 cut(s) 321, 335
TseI GCWGC 1 cut(s) 91
TspDTI ATGAA 2 cut(s) 37, 159
VspI ATTAAT 1 cut(s) 335
XapI RAATTY 1 cut(s) 224
XceI RCATGY 1 cut(s) 190
XhoI CTCGAG 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.