Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0002968

Physical Location & Seq

Genomic Coordinates Forward (+)

35241887 .. 35244418

Gene Structure 2532 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1258300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2532 bp

ATGCTCTCTGGTCGTAGCCGTTGCGTTAGAACTGTTGGAACTCGCGTCGGACCTCATAACTCTACTACCGGAAAGGCGCTTGCCTGTGAGCCCACTAGCCATTTTCCTGGGCACCCATTGATCTTCGCCGAGCTCGCTGCATTTTTCTCATCGTTTTCCTCGAGAAACTCCGACCCAGGTTCTGGTTTTAGCTCTGAATTCAAAGCTCGTTCTTTTGATATTGATGGTTTGGGAGATTCAAGTAATGGGTATGATAGAGCTAGGAAAATGGGTTCTGAAAATTTCGGGGATTTGAGTCTCTTAGGGAGTAGGAAGAATGGTTGCGATAATGATCAGAGCAAAAAGTTTGGTATTTTTTATGACATTGAAGGGTCTGATGGTGGAGAAGAAGAGAGTAGCGGTGATGAGGATGATGACGGTGGTGGTGGTGGTGATGGGGATTTAACGGTGTTGGATTCGTCGAATCGGGTTCACGAAAAAGGCGAAAACTTTAGGAGAGTTGATGGATATGAAGAGGAATTCAGGCATCCATTGGTGAGAGAGGTTTGTAGGTTAATTGAGTTTAGGTCAGGTTGGAGCCCTAAGCTTGAAGGGGAATTAAAGAACTTGTTAAGAAGCTTGAAGCCGAGGCAAGTTTGCGCCGTGTTGAAGTCTCAGAGTGATGAGAGAGTTGCTTTGAAGTTTTTCTATTGGGCTGATAGGCAGTGGAGGTATAAGCATCGCCCAGTTGTGTACTATGCAATGCTTGAGGTGTTAAGCAAGACTAAGTTGTGTCAAGGTGCTAAGAGGGTTCTCCGTCTTATGGCACGTAGAGGAATTGAGCGTAGCCCTGAAGCTTTTGGTTGTGTGATGGTGTCTTATAGCAGGGCGGGCAAGTTGAGGCATGCCCTGCGTGTGCTGACATTGATGCAGAAGGCCGGAGTTGAGCTTAATGTGTCAATGTGCAACACTGCAATTCATGTTTTGGTGATGGGGAATAAGTTGGAGAAGGCTTTGAGGGTGTTGGAAAGGATGCAACTTGTTGGAATTACACCAGATGTTGTCACCTATAATTGCTTGATAAAGGGGTATTGTGATGTGCATCGCGTCCAGGATGCATTGGAGCTGATTGATGAAATGCCGAAAAGAGGGTGCGTGCCGGATAAAGTTAGTTACTATACTGTGATGGGTTTCCTCTGTAAGGAGAATAGGGTCGAAGAAGTGAGGGGATTGATGGAGAAGATGATTAAGGACAGTGGATTGTTACCGGACCAGGTCACTTATAATAATCTTGTCCATGTGCTTTCTAAGCACGGTTATGGAGATGAGGCTCTTGAGTTTTTAAGGGAAGCTGAAGAGAGGGGACTTAGGTTTGATAAGGTTGGGTATAGTGCAATAGTTCATTCATTCTGTAAGGACGGTAGGATTGATAAGGCAAAAGAAATTGTGAATGAAATGTTCTCAAAAGGTTGCACTCCGGATGTTGTAACATACACTGCTGTTCTTGATGGGTATTGCCGGCTTGGGAAGGTGGATCAAGCTAAAAAGATGCTTCAACACATGTACAAGCATGGTTGCAGGCCTAATACCGTATCATATACAGCCTTATTAAACGGGCTTTGTCGTAGTCAAAACTCATTAGAGGCAAGAGAGATGATGAAAGTGAGTGAGGAAGAATGGTGGACTCCGAATGCCATCACTTATAGTGTTGTGATGCATGGGTTCCGTAGGGAAGGGAAATTAGTTGAGGCTTGTGATATCGTCAGAGAGATGGTTAATATGGGTTTTTTCCCAAACCCAGTTGAAATTAATTTACTAATTCAGTCTCTTTGCCGAGAAGGGAAGATAACTGAGGCTAAACGTTTCATGGAGGAGTGTCTAAGTAAGGGATGTGCAGTTAATGTTATAAATTTTACAACTGTGATTCATGGATATTGTCAAAAGGATGACTTGGACGCTGCTTTGTCATTGCTTGATGACATGTATCTGAGCAACAAACACCCAGACACGGTCACTTATACAACAGTGATCCATGCGTTGGGAAAGAATGGTCGAATACAAGAGGCGACCGAACTTATGAAGAAGATGCTGGGTAAAGGGTTGGATCCGACTCCAGTAACATACAGGACAGTCATCCACTGGTATTGTCAAATGGGTAAGGTTGACGAGTTATTACAATTATTGGAGAAGATGTTCTTAAGACAAAATTGCAAAACAGCATATAATCAAGTGATTGAGAAGCTTTGTAGTTTTGGGAACCTTGAGGAGGCTGATAAACTATTAGGTAAGGTGTTGAGAACAGCTTCTAGAGTCGATGCTAAGACGTGCCATTCTCTTATGGGTGGTTACTTAAGAAAAGGGGATCCTCTGTCAGCATACAAAGTGGCTTGTCGAATGTTCAACCGGAATCTTATTCCTGATTTAAAGTTATGTGAGAAGGTGACCAAGAGACTGATGTTAGATGGAAACTCCAAAGAGGCAGACAACCTAATGTTACGTTTTGTTGAGCGTGGATGCATATCAAACCAATATCAGGAACATTTGCAAAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016070 GO:0016787 GO:0016788 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

844

Amino Acids

95.2

Weight (kDa)

8.41

Isoelectric Point (pI)

36.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	253 - 369	1.9e-10	Fungal tetratrico peptide repeats
PPR_long	PF17177	284 - 443	1.1e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	299 - 357	2e-12	Pentatricopeptide repeat domain
PPR_2	PF13041	311 - 359	8.2e-12	PPR repeat family
PPR_3	PF13812	342 - 392	1.7e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	342 - 373	9.5e-12	PPR repeat
PPR_2	PF13041	345 - 394	1.4e-16	PPR repeat family
PPR	PF01535	348 - 378	1.5e-07	PPR repeat
PPR_2	PF13041	380 - 422	2.5e-06	PPR repeat family
PPR_long	PF17177	407 - 569	1.8e-10	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	416 - 465	9.2e-09	PPR repeat family
PPR_3	PF13812	441 - 496	7.7e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	447 - 479	3e-08	PPR repeat
PPR_2	PF13041	453 - 500	1.5e-14	PPR repeat family
PPR_3	PF13812	475 - 531	2.6e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	482 - 514	2.9e-12	PPR repeat
PPR_2	PF13041	486 - 535	1.4e-18	PPR repeat family
PPR	PF01535	489 - 519	4.8e-07	PPR repeat
PPR_long	PF17177	609 - 725	7.3e-07	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	622 - 654	2.5e-08	PPR repeat
PPR_2	PF13041	627 - 673	3.8e-12	PPR repeat family
PPR_3	PF13812	649 - 708	1.6e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	660 - 689	4.2e-09	PPR repeat
PPR_2	PF13041	661 - 706	2.3e-12	PPR repeat family
PPR	PF01535	664 - 694	1.1e-07	PPR repeat
PPR_1	PF12854	692 - 724	2.2e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002968)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30290 AT1G30290
fragaria_vesca	FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050
malus_domestica	MD10G1258300.v1.1
prunus_persica	Prupe.4G085500_v2.0.a1 Prupe.4G085500_v2.0.a1
pyrus_communis	pycom05g26000 pycom10g21460
rosa_chinensis	RchiOBHm_Chr3g0490541 RchiOBHm_Chr3g0490551 RchiOBHm_Chr5g0014911
rosa_laevigata	RLG00000022885 RLG00000032150
rosa_multiflora	Rmu_sc0003862.1_g000007 Rmu_sc0013610.1_g000002
rosa_roxburghii	Rroxscaffold_1G00061660 Rroxscaffold_6G00393420
rosa_rugosa	Rorug03G0243900 Rorug05G0021400
rosa_samantha	Rh3AG293300 Rh3AG293400 Rh3BG329800 Rh3CG327300 Rh3CG327400 Rh3DG326700 Rh5AG117100 Rh5BG114100 Rh5CG125600 Rh5DG113500
rosa_wichuraiana	Rw3G025970 Rw5G010080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1263, 1886
AccB1I	GGYRCC	1 cut(s)	111
AccB7I	CCANNNNNTGG	2 cut(s)	182, 2017
AccII	CGCG	2 cut(s)	45, 1086
AccIII	TCCGGA	1 cut(s)	1456
AciI	CCGC	2 cut(s)	399, 869
AclI	AACGTT	1 cut(s)	1840
AclWI	GGATC	6 cut(s)	1521, 2002, 2078, 2091, 2336, 2349
AcsI	RAATTY	4 cut(s)	197, 280, 518, 1888
AcuI	CTGAAG	2 cut(s)	852, 1353
AfaI	GTAC	2 cut(s)	734, 1544
AfiI	CCNNNNNNNGG	8 cut(s)	182, 802, 1127, 1180, 1987, 2017, 2245, 2512
AflII	CTTAAG	2 cut(s)	2176, 2329
AflIII	ACRYGT	2 cut(s)	1539, 1959
AjiI	CACGTC	1 cut(s)	2304
AjnI	CCWGG	4 cut(s)	106, 175, 1089, 1251
AloI	GAACNNNNNNTCC	4 cut(s)	256, 288, 2156, 2188
Alw21I	GWGCWC	1 cut(s)	135
Alw26I	GTCTC	4 cut(s)	302, 657, 1809, 2422
AlwI	GGATC	6 cut(s)	1521, 2002, 2078, 2091, 2336, 2349
AlwNI	CAGNNNCTG	1 cut(s)	182
Ama87I	CYCGRG	1 cut(s)	160
Aor13HI	TCCGGA	1 cut(s)	1456
AoxI	GGCC	2 cut(s)	915, 1559
ApeKI	GCWGC	2 cut(s)	137, 1937
ApoI	RAATTY	4 cut(s)	197, 280, 518, 1888
AseI	ATTAAT	1 cut(s)	1788
Asp700I	GAANNNNTTC	3 cut(s)	683, 2171, 2281
AspLEI	GCGC	2 cut(s)	79, 641
AspS9I	GGNCC	2 cut(s)	50, 1249
AsuHPI	GGTGA	6 cut(s)	413, 443, 547, 979, 1036, 2431
AvaI	CYCGRG	1 cut(s)	160
AvaII	GGWCC	2 cut(s)	50, 1249
BaeGI	GKGCMC	1 cut(s)	114
BamHI	GGATCC	2 cut(s)	2083, 2341
BanI	GGYRCC	1 cut(s)	111
BanII	GRGCYC	3 cut(s)	93, 135, 581
Bbv12I	GWGCWC	1 cut(s)	135
BbvI	GCAGC	2 cut(s)	124, 1924
BceAI	ACGGC	2 cut(s)	3, 626
BcgI	CGANNNNNNTGC	2 cut(s)	119, 153
BciT130I	CCWGG	4 cut(s)	108, 177, 1091, 1253
BclI	TGATCA	1 cut(s)	331
BcoDI	GTCTC	4 cut(s)	302, 657, 1809, 2422
BfaI	CTAG	3 cut(s)	96, 261, 2286
BfoI	RGCGCY	1 cut(s)	80
BfrI	CTTAAG	2 cut(s)	2176, 2329
BisI	GCNGC	2 cut(s)	138, 1938
BlsI	GCNGC	2 cut(s)	139, 1939
Bme1390I	CCNGG	4 cut(s)	108, 177, 1091, 1253
Bme18I	GGWCC	2 cut(s)	50, 1249
BmeT110I	CYCGRG	1 cut(s)	160
BmgBI	CACGTC	1 cut(s)	2304
BmgT120I	GGNCC	2 cut(s)	50, 1249
BmiI	GGNNCC	6 cut(s)	113, 578, 1703, 2085, 2237, 2343
BmrFI	CCNGG	4 cut(s)	108, 177, 1091, 1253
BmrI	ACTGGG	2 cut(s)	719, 1772
BmuI	ACTGGG	2 cut(s)	719, 1772
BpmI	CTGGAG	1 cut(s)	2076
Bpu10I	CCTNAGC	1 cut(s)	582
BpuEI	CTTGAG	3 cut(s)	767, 1334, 2261
BsaAI	YACGTR	1 cut(s)	809
BsaBI	GATNNNNATC	3 cut(s)	330, 1080, 2497
BsaJI	CCNNGG	3 cut(s)	107, 175, 626
BsaWI	WCCGGW	4 cut(s)	68, 1246, 1456, 2382
BsaXI	ACNNNNNCTCC	2 cut(s)	2156, 2186
Bsc4I	CCNNNNNNNGG	8 cut(s)	182, 802, 1127, 1180, 1987, 2017, 2245, 2512
Bse118I	RCCGGY	1 cut(s)	1497
Bse1I	ACTGG	4 cut(s)	725, 1778, 2093, 2123
Bse3DI	GCAATG	2 cut(s)	747, 1946
Bse8I	GATNNNNATC	3 cut(s)	330, 1080, 2497
BseAI	TCCGGA	1 cut(s)	1456
BseBI	CCWGG	4 cut(s)	108, 177, 1091, 1253
BseDI	CCNNGG	3 cut(s)	107, 175, 626
BseGI	GGATG	9 cut(s)	415, 526, 1017, 1099, 1465, 1874, 1930, 2112, 2498
BseJI	GATNNNNATC	3 cut(s)	330, 1080, 2497
BseLI	CCNNNNNNNGG	8 cut(s)	182, 802, 1127, 1180, 1987, 2017, 2245, 2512
BseMI	GCAATG	2 cut(s)	747, 1946
BseMII	CTCAG	3 cut(s)	668, 1821, 1958
BseNI	ACTGG	4 cut(s)	725, 1778, 2093, 2123
BseRI	GAGGAG	2 cut(s)	1865, 2258
BseSI	GKGCMC	1 cut(s)	114
BseXI	GCAGC	2 cut(s)	124, 1924
BseYI	CCCAGC	1 cut(s)	2068
BsgI	GTGCAG	1 cut(s)	1893
Bsh1236I	CGCG	2 cut(s)	45, 1086
Bsh1285I	CGRYCG	1 cut(s)	2049
BshFI	GGCC	2 cut(s)	917, 1561
BshNI	GGYRCC	1 cut(s)	111
BsiEI	CGRYCG	1 cut(s)	2049
BsiHKAI	GWGCWC	1 cut(s)	135
BsiHKCI	CYCGRG	1 cut(s)	160
BsiSI	CCGG	7 cut(s)	69, 918, 1139, 1247, 1457, 1498, 2383
BslFI	GGGAC	1 cut(s)	1356
BslI	CCNNNNNNNGG	8 cut(s)	182, 802, 1127, 1180, 1987, 2017, 2245, 2512
BsmAI	GTCTC	4 cut(s)	302, 657, 1809, 2422
BsmFI	GGGAC	1 cut(s)	1356
BsmI	GAATGC	1 cut(s)	1675
BsnI	GGCC	2 cut(s)	917, 1561
BsoBI	CYCGRG	1 cut(s)	160
Bsp1286I	GDGCHC	4 cut(s)	93, 114, 135, 581
Bsp13I	TCCGGA	1 cut(s)	1456
Bsp1407I	TGTACA	1 cut(s)	1542
Bsp143I	GATC	6 cut(s)	120, 331, 1513, 2007, 2083, 2341
BspACI	CCGC	2 cut(s)	399, 869
BspANI	GGCC	2 cut(s)	917, 1561
BspCNI	CTCAG	3 cut(s)	667, 1822, 1959
BspEI	TCCGGA	1 cut(s)	1456
BspFNI	CGCG	2 cut(s)	45, 1086
BspLI	GGNNCC	6 cut(s)	113, 578, 1703, 2085, 2237, 2343
BspPI	GGATC	6 cut(s)	1521, 2002, 2078, 2091, 2336, 2349
BspT107I	GGYRCC	1 cut(s)	111
BspTI	CTTAAG	2 cut(s)	2176, 2329
BsrDI	GCAATG	2 cut(s)	747, 1946
BsrFI	RCCGGY	1 cut(s)	1497
BsrGI	TGTACA	1 cut(s)	1542
BsrI	ACTGG	4 cut(s)	725, 1778, 2093, 2123
BssAI	RCCGGY	1 cut(s)	1497
BssECI	CCNNGG	3 cut(s)	107, 175, 626
BssMI	GATC	6 cut(s)	120, 331, 1513, 2007, 2083, 2341
Bst2UI	CCWGG	4 cut(s)	108, 177, 1091, 1253
Bst6I	CTCTTC	3 cut(s)	384, 507, 1329
BstAFI	CTTAAG	2 cut(s)	2176, 2329
BstAPI	GCANNNNNTGC	1 cut(s)	889
BstAUI	TGTACA	1 cut(s)	1542
BstBAI	YACGTR	1 cut(s)	809
BstC8I	GCNNGC	7 cut(s)	81, 135, 871, 885, 1136, 1499, 1559
BstEII	GGTNACC	1 cut(s)	2419
BstENI	CCTNNNNNAGG	2 cut(s)	1178, 2243
BstF5I	GGATG	9 cut(s)	415, 526, 1017, 1099, 1465, 1874, 1930, 2112, 2498
BstFNI	CGCG	2 cut(s)	45, 1086
BstH2I	RGCGCY	1 cut(s)	80
BstHHI	GCGC	2 cut(s)	79, 641
BstKTI	GATC	6 cut(s)	123, 334, 1516, 2010, 2086, 2344
BstMAI	GTCTC	4 cut(s)	302, 657, 1809, 2422
BstMBI	GATC	6 cut(s)	120, 331, 1513, 2007, 2083, 2341
BstMCI	CGRYCG	1 cut(s)	2049
BstMWI	GCNNNNNNNGC	4 cut(s)	134, 870, 889, 1288
BstNI	CCWGG	4 cut(s)	108, 177, 1091, 1253
BstNSI	RCATGY	3 cut(s)	887, 1543, 1963
BstPI	GGTNACC	1 cut(s)	2419
BstSCI	CCNGG	4 cut(s)	106, 175, 1089, 1251
BstSLI	GKGCMC	1 cut(s)	114
BstUI	CGCG	2 cut(s)	45, 1086
BstV1I	GCAGC	2 cut(s)	124, 1924
BstX2I	RGATCY	2 cut(s)	2083, 2341
BstXI	CCANNNNNNTGG	1 cut(s)	107
BstYI	RGATCY	2 cut(s)	2083, 2341
BsuRI	GGCC	2 cut(s)	917, 1561
BtgZI	GCGATG	2 cut(s)	704, 1067
BtrI	CACGTC	1 cut(s)	2304
BtsCI	GGATG	9 cut(s)	415, 526, 1017, 1099, 1465, 1874, 1930, 2112, 2498
BtsI	GCAGTG	3 cut(s)	710, 948, 1473
BtsIMutI	CAGTG	6 cut(s)	710, 948, 1240, 1473, 2010, 2116
Cac8I	GCNNGC	7 cut(s)	81, 135, 871, 885, 1136, 1499, 1559
CaiI	CAGNNNCTG	1 cut(s)	182
CfoI	GCGC	2 cut(s)	79, 641
Cfr10I	RCCGGY	1 cut(s)	1497
Cfr13I	GGNCC	2 cut(s)	50, 1249
CseI	GACGC	3 cut(s)	34, 1075, 1943
CsiI	ACCWGGT	1 cut(s)	1251
Csp6I	GTAC	2 cut(s)	733, 1543
CspCI	CAANNNNNGTGG	2 cut(s)	2105, 2140
CviQI	GTAC	2 cut(s)	733, 1543
DpnI	GATC	6 cut(s)	122, 333, 1515, 2009, 2085, 2343
DpnII	GATC	6 cut(s)	120, 331, 1513, 2007, 2083, 2341
DraI	TTTAAA	1 cut(s)	2403
Eam1104I	CTCTTC	3 cut(s)	384, 507, 1329
EarI	CTCTTC	3 cut(s)	384, 507, 1329
Ecl136II	GAGCTC	1 cut(s)	133
Eco147I	AGGCCT	1 cut(s)	1561
Eco24I	GRGCYC	3 cut(s)	93, 135, 581
Eco32I	GATATC	1 cut(s)	1738
Eco47I	GGWCC	2 cut(s)	50, 1249
Eco53kI	GAGCTC	1 cut(s)	133
Eco57I	CTGAAG	2 cut(s)	852, 1353
Eco88I	CYCGRG	1 cut(s)	160
Eco91I	GGTNACC	1 cut(s)	2419
EcoICRI	GAGCTC	1 cut(s)	133
EcoNI	CCTNNNNNAGG	2 cut(s)	1178, 2243
EcoO65I	GGTNACC	1 cut(s)	2419
EcoRI	GAATTC	2 cut(s)	197, 518
EcoRII	CCWGG	4 cut(s)	106, 175, 1089, 1251
EcoRV	GATATC	1 cut(s)	1738
EcoT22I	ATGCAT	3 cut(s)	1099, 1698, 2498
EcoT38I	GRGCYC	3 cut(s)	93, 135, 581
FalI	AAGNNNNNCTT	4 cut(s)	1913, 1945, 2159, 2191
FaqI	GGGAC	1 cut(s)	1356
FauI	CCCGC	1 cut(s)	862
FbaI	TGATCA	1 cut(s)	331
Fnu4HI	GCNGC	2 cut(s)	138, 1938
FokI	GGATG	9 cut(s)	422, 513, 1024, 1106, 1472, 1881, 1937, 2099, 2505
FriOI	GRGCYC	3 cut(s)	93, 135, 581
Fsp4HI	GCNGC	2 cut(s)	138, 1938
FspBI	CTAG	3 cut(s)	96, 261, 2286
GlaI	GCGC	2 cut(s)	78, 640
GluI	GCNGC	2 cut(s)	138, 1938
GsaI	CCCAGC	1 cut(s)	2072
GsuI	CTGGAG	1 cut(s)	2076
HaeII	RGCGCY	1 cut(s)	80
HaeIII	GGCC	2 cut(s)	917, 1561
HapII	CCGG	7 cut(s)	69, 918, 1139, 1247, 1457, 1498, 2383
HgaI	GACGC	3 cut(s)	34, 1075, 1943
HhaI	GCGC	2 cut(s)	79, 641
Hin6I	GCGC	2 cut(s)	77, 639
HinP1I	GCGC	2 cut(s)	77, 639
HincII	GTYRAC	1 cut(s)	2143
HindII	GTYRAC	1 cut(s)	2143
HindIII	AAGCTT	4 cut(s)	584, 616, 834, 2219
HinfI	GANTC	9 cut(s)	236, 295, 455, 463, 1663, 1903, 2089, 2289, 2386
HpaII	CCGG	7 cut(s)	69, 918, 1139, 1247, 1457, 1498, 2383
HphI	GGTGA	6 cut(s)	413, 443, 547, 979, 1036, 2431
Hpy166II	GTNNAC	4 cut(s)	472, 733, 1662, 2143
Hpy188III	TCNNGA	8 cut(s)	162, 473, 1313, 1457, 1484, 2286, 2396, 2513
Hpy8I	GTNNAC	4 cut(s)	472, 733, 1662, 2143
Hpy99I	CGWCG	2 cut(s)	50, 463
HpyAV	CCTTC	8 cut(s)	362, 584, 907, 982, 1501, 1706, 1811, 2410
HpyCH4IV	ACGT	4 cut(s)	808, 1840, 2303, 2476
HpyF10VI	GCNNNNNNNGC	4 cut(s)	134, 870, 889, 1288
HpySE526I	ACGT	4 cut(s)	808, 1840, 2303, 2476
HspAI	GCGC	2 cut(s)	77, 639
Kpn2I	TCCGGA	1 cut(s)	1456
KroI	GCCGGC	1 cut(s)	1497
KroNI	GCCGGC	1 cut(s)	1499
Ksp22I	TGATCA	1 cut(s)	331
Kzo9I	GATC	6 cut(s)	120, 331, 1513, 2007, 2083, 2341
LmnI	GCTCC	2 cut(s)	576, 1102
Lsp1109I	GCAGC	2 cut(s)	124, 1924
MabI	ACCWGGT	1 cut(s)	1251
MaeI	CTAG	3 cut(s)	96, 261, 2286
MaeII	ACGT	4 cut(s)	808, 1840, 2303, 2476
MalI	GATC	6 cut(s)	122, 333, 1515, 2009, 2085, 2343
MboI	GATC	6 cut(s)	120, 331, 1513, 2007, 2083, 2341
MflI	RGATCY	2 cut(s)	2083, 2341
MhlI	GDGCHC	4 cut(s)	93, 114, 135, 581
MlyI	GAGTC	4 cut(s)	304, 1657, 2083, 2298
Mph1103I	ATGCAT	3 cut(s)	1099, 1698, 2498
MroI	TCCGGA	1 cut(s)	1456
MroNI	GCCGGC	1 cut(s)	1497
MroXI	GAANNNNTTC	3 cut(s)	683, 2171, 2281
MslI	CAYNNNNRTG	1 cut(s)	1296
MspCI	CTTAAG	2 cut(s)	2176, 2329
MspI	CCGG	7 cut(s)	69, 918, 1139, 1247, 1457, 1498, 2383
MspR9I	CCNGG	4 cut(s)	108, 177, 1091, 1253
Mva1269I	GAATGC	1 cut(s)	1675
MvaI	CCWGG	4 cut(s)	108, 177, 1091, 1253
MvnI	CGCG	2 cut(s)	45, 1086
MwoI	GCNNNNNNNGC	4 cut(s)	134, 870, 889, 1288
NaeI	GCCGGC	1 cut(s)	1499
NdeII	GATC	6 cut(s)	120, 331, 1513, 2007, 2083, 2341
NgoMIV	GCCGGC	1 cut(s)	1497
NlaIV	GGNNCC	6 cut(s)	113, 578, 1703, 2085, 2237, 2343
NmeAIII	GCCGAG	3 cut(s)	154, 651, 1838
NmuCI	GTSAC	4 cut(s)	1042, 1255, 1990, 2419
NsiI	ATGCAT	3 cut(s)	1099, 1698, 2498
NspI	RCATGY	3 cut(s)	887, 1543, 1963
PaeI	GCATGC	1 cut(s)	887
PaeR7I	CTCGAG	1 cut(s)	160
PceI	AGGCCT	1 cut(s)	1561
PciI	ACATGT	2 cut(s)	1539, 1959
PcsI	WCGNNNNNNNCGW	2 cut(s)	158, 480
PctI	GAATGC	1 cut(s)	1675
PdiI	GCCGGC	1 cut(s)	1499
PdmI	GAANNNNTTC	3 cut(s)	683, 2171, 2281
PfeI	GAWTC	5 cut(s)	236, 455, 463, 1903, 2386
PflFI	GACNNNGTC	2 cut(s)	1253, 1988
PflMI	CCANNNNNTGG	2 cut(s)	182, 2017
PfoI	TCCNGGA	1 cut(s)	1089
PkrI	GCNGC	2 cut(s)	139, 1939
PleI	GAGTC	4 cut(s)	303, 1657, 2083, 2297
PpsI	GAGTC	4 cut(s)	303, 1657, 2083, 2297
Ppu21I	YACGTR	1 cut(s)	809
PscI	ACATGT	2 cut(s)	1539, 1959
PshBI	ATTAAT	1 cut(s)	1788
PsiI	TTATAA	2 cut(s)	1263, 1886
Psp124BI	GAGCTC	1 cut(s)	135
Psp1406I	AACGTT	1 cut(s)	1840
Psp6I	CCWGG	4 cut(s)	106, 175, 1089, 1251
PspEI	GGTNACC	1 cut(s)	2419
PspFI	CCCAGC	1 cut(s)	2068
PspGI	CCWGG	4 cut(s)	106, 175, 1089, 1251
PspN4I	GGNNCC	6 cut(s)	113, 578, 1703, 2085, 2237, 2343
PspPI	GGNCC	2 cut(s)	50, 1249
PstNI	CAGNNNCTG	1 cut(s)	182
PsuI	RGATCY	2 cut(s)	2083, 2341
PsyI	GACNNNGTC	2 cut(s)	1253, 1988
RsaI	GTAC	2 cut(s)	734, 1544
RsaNI	GTAC	2 cut(s)	733, 1543
RseI	CAYNNNNRTG	1 cut(s)	1296
SacI	GAGCTC	1 cut(s)	135
SatI	GCNGC	2 cut(s)	138, 1938
Sau3AI	GATC	6 cut(s)	120, 331, 1513, 2007, 2083, 2341
Sau96I	GGNCC	2 cut(s)	50, 1249
SchI	GAGTC	4 cut(s)	304, 1657, 2083, 2298
ScrFI	CCNGG	4 cut(s)	108, 177, 1091, 1253
SduI	GDGCHC	4 cut(s)	93, 114, 135, 581
SexAI	ACCWGGT	1 cut(s)	1251
Sfr274I	CTCGAG	1 cut(s)	160
SinI	GGWCC	2 cut(s)	50, 1249
SlaI	CTCGAG	1 cut(s)	160
SmiMI	CAYNNNNRTG	1 cut(s)	1296
SmlI	CTYRAG	6 cut(s)	160, 746, 1313, 2176, 2240, 2329
SmoI	CTYRAG	6 cut(s)	160, 746, 1313, 2176, 2240, 2329
SphI	GCATGC	1 cut(s)	887
SseBI	AGGCCT	1 cut(s)	1561
SsiI	CCGC	2 cut(s)	399, 869
SspMI	CTAG	3 cut(s)	96, 261, 2286
SstI	GAGCTC	1 cut(s)	135
StuI	AGGCCT	1 cut(s)	1561
StyD4I	CCNGG	4 cut(s)	106, 175, 1089, 1251
TaiI	ACGT	4 cut(s)	811, 1843, 2306, 2479
TaqI	TCGA	6 cut(s)	161, 461, 1194, 2032, 2292, 2371
TaqII	GACCGA	1 cut(s)	2063
TatI	WGTACW	2 cut(s)	732, 1542
TfiI	GAWTC	5 cut(s)	236, 455, 463, 1903, 2386
TscAI	CASTG	6 cut(s)	710, 955, 1240, 1480, 2010, 2123
TseFI	GTSAC	4 cut(s)	1042, 1255, 1990, 2419
TseI	GCWGC	2 cut(s)	137, 1937
Tsp45I	GTSAC	4 cut(s)	1042, 1255, 1990, 2419
TspGWI	ACGGA	2 cut(s)	785, 1694
TspRI	CASTG	6 cut(s)	710, 955, 1240, 1480, 2010, 2123
Tth111I	GACNNNGTC	2 cut(s)	1253, 1988
Van91I	CCANNNNNTGG	2 cut(s)	182, 2017
Vha464I	CTTAAG	2 cut(s)	2176, 2329
VpaK11BI	GGWCC	2 cut(s)	50, 1249
VspI	ATTAAT	1 cut(s)	1788
XagI	CCTNNNNNAGG	2 cut(s)	1178, 2243
XapI	RAATTY	4 cut(s)	197, 280, 518, 1888
XbaI	TCTAGA	1 cut(s)	2285
XceI	RCATGY	3 cut(s)	887, 1543, 1963
XhoI	CTCGAG	1 cut(s)	160
XmnI	GAANNNNTTC	3 cut(s)	683, 2171, 2281
XspI	CTAG	3 cut(s)	96, 261, 2286
Zsp2I	ATGCAT	3 cut(s)	1099, 1698, 2498

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.