Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0002968

Physical Location & Seq

Genomic Coordinates Reverse (-)

10684403 .. 10688337

Gene Structure 3935 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG114100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2481 bp

ATGGTCTCTATTCGTAGCTACATTCGAACCGGAGGAGCAGCTCGGCTCAGACCTCATAACTCGTGTGAGTCCACCACATCTTTCTCTCACTACCCTTTAACTTTTGTTGAGCTCCCTCAATTTTTCTCATCAGTTAGCTCAACAAACTCAAGTTCAGATTTTTGCTCTGGAATTAGACCTCATTCTGTTGAAAGTTATGATTTTGATGATTCTAATAACGGGAAAGGTAGTTTTAGGAAAATGGGTAATCGAAATACGGCGGGTTTGAGTTTCTTAGGAGTTGCCCAGAGTGACTGTGAAAACCCCCAAAGTGAAAGTTTCAAACTTTTTGAAGTTTCAGATAGCGGAGAAGAGAATAATAGTGATGGTGATGATGATGATGATGAGTTAATGATGTTGAATTCTAATAGGGGTTGTGAGCAAAAAGAGAATCTGGGTAGAGTTGAAGGAGATGGGGATGAGGATGAATATAGACACCCTTTGGTGAGAGAGGTTTGCAGGTTAATTGAGCTTAGAACAGCTTGGACCCCAAAGTTTGAAGGTGAGTTAAGGCACTTGTTCCGAAGCCTGAAGCCGAAGCAGGTCTGTGCCGTTTTGAAGTCTCAGGCTGATGAAAGAGTTGCTTTGAAGATTTTTTACTGGGCTGATAGGCAGTGGCGGTATAAGCATGACCCGATTGTGTACTATGCAATGCTTGAGGTGCTTGGCAAGACTAAGTTGTGCCAAGGTGCTAGGAGGGTTCTTAGGTTAATGGCCCGTAGGCGGATTGAGCTTCGTTCTGAAGCTTTTGGCCATGTGATGGTGTCATATAGTCGAGCGGGCAAGTTGAGGCACGCGTTGCGTGTGTTGACTTTGATGCAGAAAGCGGGGCTCGAGCTTGATTTGTCAATGTGTAATACTGCAATTTATGTTTTGGTTAAGGGTAATAAGTTGGAGAAGGCGTTGAGGACCTTGGAAAGGATGAAGCTTGTTGGGATTGCACCAAATGTAGTCACTTATAACTGTTTGATAAAGGGGTATTGTGATCTGCATCGTGTAGAAGATGCAATCCAGGTGATTGATGAAATGCCGAGGAAGGGGTGTGCTCCTGATAAAGTTAGTTACTATACCGTGATGGATTTCCTTTGTAAGGAGAAGAGGGTCAAAGAAGTGAGGGACTTGATGGAGAAGATGATCAAGGATGGTGGGTTGTTACCAGACCAGGTCACTTATAATAATCTTGTCCATGTGCTTTGTAAGCATGGCTATGGTGATGAGGCACTTGAATTTTTAAGGGAAGCCGAAGAGAAGGGATTTCGGTTTGATAAGGTTGGGTATAGTGCAATTGTACATTCATTTTGTAAGGATGGTCGGACTGACATGGCAAAAGAAATTGTTAGTGAAATGTTCTCAAGAGGTTGCATCCCAGATGTTGTAACATACACTGCTGTTCTGAATGGGTATTGTCGACTTGGGAAGTTGGATCAAGCTAAAAAGATGCTTCAGCACATGCATAAGCATGGTTGCAAGCCAAATACTGTATCATATACGGCCTTATTAAATGGGCTTTGTCGTGGTGGGAACTCATTGGAGGCAAGGGAGATGATGAACACGAGTGAGGAAGAATGGTGGACACCAAATGCCATCACTTATAGTGTTCTGATGCATGGGTTCCGGAGGGAAGGGAAATTGGTTGAGGCTTGTGATACTGTCAAAGAGATGATTAAAAAGGGATTTTTCCCTACCCCAGTTGAAATTAATCTCTTAATTCAGTCTCTTTTCCAAGAGGGGAAGATGGATGAGGCTAAACGTTTCATGGAGGAGTGTCTAAATAAAGGTTGTGCTGTTAATGTTGTCAATTTTACTACTGTTATTCATGGATATTGTCAAAAAGATGACTTGGAAGCTGCTCTGTCGTTGCTTGATGACATGTATCTCAGCAATAAACACCCAGATGCAGTCACTTATACAGCAATGATCAGTGCATTGGGAAAGAAAGGTAGAATACAAGACGCAACTGAACTTATGATGAAGATGCTGAATAAGGGTATTGACCCGACTCCCGTCACATACAGGACGATCATCCATTGGTATTGTAAGATGAGTAGGGTGGATGATTTGTTGAAATTATTGGAGAAGATGTTCTTAAGGCAAAATTGCAAAACAGCATATAATCAAGTGATTGAGAAGCTTTGTAGTTTTGGGAACTTCGAGGACGCTGATAAACTATTGGGTAAGGTGTTGAGAACAGCTTCAAGAGTTGATGCTAAAACATGCCATGTTGTTATGGATGGTTATTTGAGAAAAGGGATTCCTCTGTCAGCATACAAAGTGGCTTGTCGAATGTTCAATCGGAATCTGATTCCTGATTTAAAGTTGTGTGAGAAGGTGACGAAGAGACTGATGCTAGAAGGAAACTCCAAAGAGGCAGACAACCTTATGTTACGGTTTGTTGAGCGTGGATGCATATCAAACCAACATCAGGAACATCTGGAAAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016070 GO:0016787 GO:0016788 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

826

Amino Acids

94.18

Weight (kDa)

8.34

Isoelectric Point (pI)

33.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	232 - 322	4.2e-07	Fungal tetratrico peptide repeats
PPR_long	PF17177	263 - 426	6.2e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	282 - 340	5e-12	Pentatricopeptide repeat domain
PPR_2	PF13041	297 - 342	3.3e-11	PPR repeat family
PPR_1	PF12854	325 - 356	1.2e-11	PPR repeat
PPR_2	PF13041	328 - 377	7.5e-17	PPR repeat family
PPR	PF01535	331 - 361	1.1e-07	PPR repeat
PPR_2	PF13041	363 - 404	5.3e-06	PPR repeat family
PPR_1	PF12854	396 - 426	1.7e-08	PPR repeat
PPR_2	PF13041	399 - 448	5.8e-10	PPR repeat family
PPR_long	PF17177	400 - 554	4.6e-12	Pentacotripeptide-repeat region of PRORP
PPR	PF01535	402 - 432	1.5e-06	PPR repeat
PPR_3	PF13812	424 - 478	9.7e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	430 - 462	8.3e-06	PPR repeat
PPR_2	PF13041	436 - 483	4.8e-13	PPR repeat family
PPR_3	PF13812	458 - 514	6.8e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	465 - 497	1.3e-11	PPR repeat
PPR_2	PF13041	469 - 518	4.4e-19	PPR repeat family
PPR	PF01535	472 - 502	8.2e-08	PPR repeat
PPR_1	PF12854	500 - 530	9.7e-08	PPR repeat
PPR_1	PF12854	605 - 637	4.4e-08	PPR repeat
PPR_2	PF13041	610 - 658	1.9e-13	PPR repeat family
PPR_3	PF13812	632 - 693	2.8e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	643 - 672	5.5e-07	PPR repeat
PPR_2	PF13041	644 - 693	3e-14	PPR repeat family
PPR	PF01535	647 - 677	6.2e-07	PPR repeat
PPR_1	PF12854	675 - 707	8.3e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002968)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30290 AT1G30290
fragaria_vesca	FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050
malus_domestica	MD10G1258300.v1.1
prunus_persica	Prupe.4G085500_v2.0.a1 Prupe.4G085500_v2.0.a1
pyrus_communis	pycom05g26000 pycom10g21460
rosa_chinensis	RchiOBHm_Chr3g0490541 RchiOBHm_Chr3g0490551 RchiOBHm_Chr5g0014911
rosa_laevigata	RLG00000022885 RLG00000032150
rosa_multiflora	Rmu_sc0003862.1_g000007 Rmu_sc0013610.1_g000002
rosa_roxburghii	Rroxscaffold_1G00061660 Rroxscaffold_6G00393420
rosa_rugosa	Rorug03G0243900 Rorug05G0021400
rosa_samantha	Rh3AG293300 Rh3AG293400 Rh3BG329800 Rh3CG327300 Rh3CG327400 Rh3DG326700 Rh5AG117100 Rh5BG114100 Rh5CG125600 Rh5DG113500
rosa_wichuraiana	Rw3G025970 Rw5G010080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	999, 1212
Acc36I	ACCTGC	2 cut(s)	489, 571
AccB7I	CCANNNNNTGG	1 cut(s)	799
AccBSI	CCGCTC	1 cut(s)	818
AccI	GTMKAC	1 cut(s)	1447
AccII	CGCG	1 cut(s)	836
AccIII	TCCGGA	1 cut(s)	1653
AciI	CCGC	6 cut(s)	260, 345, 658, 763, 818, 866
AclI	AACGTT	1 cut(s)	1789
AclWI	GGATC	1 cut(s)	1470
AcoI	YGGCCR	1 cut(s)	790
AcsI	RAATTY	2 cut(s)	400, 1265
AcuI	CTGAAG	3 cut(s)	590, 801, 1466
AfaI	GTAC	2 cut(s)	683, 1329
AfiI	CCNNNNNNNGG	6 cut(s)	762, 799, 957, 1076, 1129, 2461
AflII	CTTAAG	1 cut(s)	2125
AflIII	ACRYGT	2 cut(s)	834, 1908
AjnI	CCWGG	2 cut(s)	1050, 1200
AloI	GAACNNNNNNTCC	2 cut(s)	2105, 2137
Alw21I	GWGCWC	2 cut(s)	114, 1087
Alw26I	GTCTC	4 cut(s)	10, 606, 1758, 2371
AlwI	GGATC	1 cut(s)	1470
Ama87I	CYCGRG	1 cut(s)	872
Aor13HI	TCCGGA	1 cut(s)	1653
AoxI	GGCC	3 cut(s)	753, 790, 1530
ApeKI	GCWGC	2 cut(s)	38, 1886
ApoI	RAATTY	2 cut(s)	400, 1265
AseI	ATTAAT	1 cut(s)	1737
Asp700I	GAANNNNTTC	2 cut(s)	2120, 2230
AspS9I	GGNCC	3 cut(s)	525, 754, 948
AsuHPI	GGTGA	6 cut(s)	380, 496, 554, 1066, 1262, 2380
AsuII	TTCGAA	1 cut(s)	25
AvaI	CYCGRG	1 cut(s)	872
AvaII	GGWCC	2 cut(s)	525, 948
BalI	TGGCCA	1 cut(s)	792
BanII	GRGCYC	2 cut(s)	114, 873
BarI	GAAGNNNNNNTAC	2 cut(s)	620, 652
BauI	CACGAG	2 cut(s)	61, 1591
Bbv12I	GWGCWC	2 cut(s)	114, 1087
BbvI	GCAGC	2 cut(s)	50, 1873
BceAI	ACGGC	3 cut(s)	273, 575, 1545
BciT130I	CCWGG	2 cut(s)	1052, 1202
BclI	TGATCA	2 cut(s)	1173, 1956
BcoDI	GTCTC	4 cut(s)	10, 606, 1758, 2371
BfaI	CTAG	2 cut(s)	732, 2387
BfrI	CTTAAG	1 cut(s)	2125
BfuAI	ACCTGC	2 cut(s)	489, 571
BisI	GCNGC	2 cut(s)	39, 1887
BlsI	GCNGC	2 cut(s)	40, 1888
Bme1390I	CCNGG	2 cut(s)	1052, 1202
Bme18I	GGWCC	2 cut(s)	525, 948
BmeT110I	CYCGRG	1 cut(s)	872
BmgT120I	GGNCC	3 cut(s)	525, 754, 948
BmiI	GGNNCC	2 cut(s)	527, 1652
BmrFI	CCNGG	2 cut(s)	1052, 1202
BmrI	ACTGGG	2 cut(s)	649, 1721
BmuI	ACTGGG	2 cut(s)	649, 1721
BoxI	GACNNNNGTC	1 cut(s)	2042
Bpu14I	TTCGAA	1 cut(s)	25
BpuEI	CTTGAG	3 cut(s)	133, 716, 1375
BsaBI	GATNNNNATC	2 cut(s)	1029, 2446
BsaI	GGTCTC	1 cut(s)	10
BsaJI	CCNNGG	3 cut(s)	724, 951, 1070
BsaWI	WCCGGW	2 cut(s)	29, 1653
BsaXI	ACNNNNNCTCC	4 cut(s)	926, 956, 2105, 2135
Bsc4I	CCNNNNNNNGG	6 cut(s)	762, 799, 957, 1076, 1129, 2461
Bse1I	ACTGG	2 cut(s)	644, 1727
Bse3DI	GCAATG	2 cut(s)	696, 1959
Bse8I	GATNNNNATC	2 cut(s)	1029, 2446
BseAI	TCCGGA	1 cut(s)	1653
BseBI	CCWGG	2 cut(s)	1052, 1202
BseDI	CCNNGG	3 cut(s)	724, 951, 1070
BseJI	GATNNNNATC	2 cut(s)	1029, 2446
BseLI	CCNNNNNNNGG	6 cut(s)	762, 799, 957, 1076, 1129, 2461
BseMI	GCAATG	2 cut(s)	696, 1959
BseMII	CTCAG	3 cut(s)	61, 617, 1930
BseNI	ACTGG	2 cut(s)	644, 1727
BseRI	GAGGAG	2 cut(s)	48, 1814
BseXI	GCAGC	2 cut(s)	50, 1873
Bsh1236I	CGCG	1 cut(s)	836
BshFI	GGCC	3 cut(s)	755, 792, 1532
BsiHKAI	GWGCWC	2 cut(s)	114, 1087
BsiHKCI	CYCGRG	1 cut(s)	872
BsiSI	CCGG	2 cut(s)	30, 1654
BslFI	GGGAC	1 cut(s)	1169
BslI	CCNNNNNNNGG	6 cut(s)	762, 799, 957, 1076, 1129, 2461
BsmAI	GTCTC	4 cut(s)	10, 606, 1758, 2371
BsmFI	GGGAC	1 cut(s)	1169
BsnI	GGCC	3 cut(s)	755, 792, 1532
Bso31I	GGTCTC	1 cut(s)	10
BsoBI	CYCGRG	1 cut(s)	872
Bsp119I	TTCGAA	1 cut(s)	25
Bsp1286I	GDGCHC	3 cut(s)	114, 873, 1087
Bsp13I	TCCGGA	1 cut(s)	1653
Bsp1407I	TGTACA	1 cut(s)	1327
Bsp143I	GATC	5 cut(s)	1024, 1173, 1462, 1956, 2058
BspACI	CCGC	6 cut(s)	260, 345, 658, 763, 818, 866
BspANI	GGCC	3 cut(s)	755, 792, 1532
BspCNI	CTCAG	3 cut(s)	60, 616, 1929
BspEI	TCCGGA	1 cut(s)	1653
BspFNI	CGCG	1 cut(s)	836
BspLI	GGNNCC	2 cut(s)	527, 1652
BspMI	ACCTGC	2 cut(s)	489, 571
BspPI	GGATC	1 cut(s)	1470
BspT104I	TTCGAA	1 cut(s)	25
BspTI	CTTAAG	1 cut(s)	2125
BspTNI	GGTCTC	1 cut(s)	10
BsrBI	CCGCTC	1 cut(s)	818
BsrDI	GCAATG	2 cut(s)	696, 1959
BsrGI	TGTACA	1 cut(s)	1327
BsrI	ACTGG	2 cut(s)	644, 1727
BssECI	CCNNGG	3 cut(s)	724, 951, 1070
BssMI	GATC	5 cut(s)	1024, 1173, 1462, 1956, 2058
BssSI	CACGAG	2 cut(s)	61, 1591
BssT1I	CCWWGG	2 cut(s)	724, 951
Bst2BI	CACGAG	2 cut(s)	61, 1591
Bst2UI	CCWGG	2 cut(s)	1052, 1202
Bst4CI	ACNGT	7 cut(s)	296, 1004, 1111, 1519, 1690, 1849, 2427
Bst6I	CTCTTC	4 cut(s)	345, 1130, 1278, 2369
BstAFI	CTTAAG	1 cut(s)	2125
BstAPI	GCANNNNNTGC	1 cut(s)	838
BstAUI	TGTACA	1 cut(s)	1327
BstBI	TTCGAA	1 cut(s)	25
BstC8I	GCNNGC	3 cut(s)	820, 834, 1508
BstDEI	CTNAG	7 cut(s)	47, 274, 512, 603, 714, 743, 1916
BstENI	CCTNNNNNAGG	2 cut(s)	955, 1127
BstFNI	CGCG	1 cut(s)	836
BstKTI	GATC	5 cut(s)	1027, 1176, 1465, 1959, 2061
BstMAI	GTCTC	4 cut(s)	10, 606, 1758, 2371
BstMBI	GATC	5 cut(s)	1024, 1173, 1462, 1956, 2058
BstMWI	GCNNNNNNNGC	5 cut(s)	664, 700, 769, 838, 1237
BstNI	CCWGG	2 cut(s)	1052, 1202
BstNSI	RCATGY	3 cut(s)	1492, 1912, 2256
BstPAI	GACNNNNGTC	1 cut(s)	2042
BstSCI	CCNGG	2 cut(s)	1050, 1200
BstUI	CGCG	1 cut(s)	836
BstV1I	GCAGC	2 cut(s)	50, 1873
BsuRI	GGCC	3 cut(s)	755, 792, 1532
BtsI	GCAGTG	2 cut(s)	659, 1422
BtsIMutI	CAGTG	3 cut(s)	659, 1422, 1966
BveI	ACCTGC	2 cut(s)	489, 571
Cac8I	GCNNGC	3 cut(s)	820, 834, 1508
Cfr13I	GGNCC	3 cut(s)	525, 754, 948
CseI	GACGC	2 cut(s)	2000, 2204
CsiI	ACCWGGT	1 cut(s)	1200
Csp6I	GTAC	2 cut(s)	682, 1328
CspCI	CAANNNNNGTGG	2 cut(s)	1165, 1200
CviQI	GTAC	2 cut(s)	682, 1328
DdeI	CTNAG	7 cut(s)	47, 274, 512, 603, 714, 743, 1916
DpnI	GATC	5 cut(s)	1026, 1175, 1464, 1958, 2060
DpnII	GATC	5 cut(s)	1024, 1173, 1462, 1956, 2058
DraI	TTTAAA	1 cut(s)	2352
EaeI	YGGCCR	1 cut(s)	790
Eam1104I	CTCTTC	4 cut(s)	345, 1130, 1278, 2369
EarI	CTCTTC	4 cut(s)	345, 1130, 1278, 2369
EciI	GGCGGA	1 cut(s)	778
Ecl136II	GAGCTC	1 cut(s)	112
Eco130I	CCWWGG	2 cut(s)	724, 951
Eco24I	GRGCYC	2 cut(s)	114, 873
Eco31I	GGTCTC	1 cut(s)	10
Eco47I	GGWCC	2 cut(s)	525, 948
Eco53kI	GAGCTC	1 cut(s)	112
Eco57I	CTGAAG	3 cut(s)	590, 801, 1466
Eco88I	CYCGRG	1 cut(s)	872
EcoICRI	GAGCTC	1 cut(s)	112
EcoNI	CCTNNNNNAGG	2 cut(s)	955, 1127
EcoO109I	RGGNCCY	1 cut(s)	948
EcoRI	GAATTC	1 cut(s)	400
EcoRII	CCWGG	2 cut(s)	1050, 1200
EcoT14I	CCWWGG	2 cut(s)	724, 951
EcoT22I	ATGCAT	3 cut(s)	1494, 1647, 2447
EcoT38I	GRGCYC	2 cut(s)	114, 873
ErhI	CCWWGG	2 cut(s)	724, 951
FalI	AAGNNNNNCTT	4 cut(s)	607, 639, 2108, 2140
FaqI	GGGAC	1 cut(s)	1169
FauI	CCCGC	3 cut(s)	253, 811, 859
FbaI	TGATCA	2 cut(s)	1173, 1956
FblI	GTMKAC	1 cut(s)	1447
Fnu4HI	GCNGC	2 cut(s)	39, 1887
FriOI	GRGCYC	2 cut(s)	114, 873
Fsp4HI	GCNGC	2 cut(s)	39, 1887
FspBI	CTAG	2 cut(s)	732, 2387
GluI	GCNGC	2 cut(s)	39, 1887
HaeIII	GGCC	3 cut(s)	755, 792, 1532
HapII	CCGG	2 cut(s)	30, 1654
HgaI	GACGC	2 cut(s)	2000, 2204
HincII	GTYRAC	2 cut(s)	849, 1448
HindII	GTYRAC	2 cut(s)	849, 1448
HindIII	AAGCTT	3 cut(s)	783, 965, 2168
HinfI	GANTC	7 cut(s)	68, 209, 430, 2038, 2290, 2335, 2341
HpaII	CCGG	2 cut(s)	30, 1654
HphI	GGTGA	6 cut(s)	380, 496, 554, 1066, 1262, 2380
Hpy166II	GTNNAC	5 cut(s)	72, 682, 849, 1448, 1611
Hpy188III	TCNNGA	8 cut(s)	168, 1088, 1392, 1654, 2235, 2345, 2462, 2471
Hpy8I	GTNNAC	5 cut(s)	72, 682, 849, 1448, 1611
HpyAV	CCTTC	8 cut(s)	440, 533, 931, 1069, 1282, 1655, 2359, 2384
HpyCH4III	ACNGT	7 cut(s)	296, 1004, 1111, 1519, 1690, 1849, 2427
HpyCH4IV	ACGT	1 cut(s)	1789
HpyF10VI	GCNNNNNNNGC	5 cut(s)	664, 700, 769, 838, 1237
HpyF3I	CTNAG	7 cut(s)	47, 274, 512, 603, 714, 743, 1916
HpySE526I	ACGT	1 cut(s)	1789
Kpn2I	TCCGGA	1 cut(s)	1653
Ksp22I	TGATCA	2 cut(s)	1173, 1956
Kzo9I	GATC	5 cut(s)	1024, 1173, 1462, 1956, 2058
LmnI	GCTCC	3 cut(s)	35, 117, 1090
Lsp1109I	GCAGC	2 cut(s)	50, 1873
MabI	ACCWGGT	1 cut(s)	1200
MaeI	CTAG	2 cut(s)	732, 2387
MaeII	ACGT	1 cut(s)	1789
MalI	GATC	5 cut(s)	1026, 1175, 1464, 1958, 2060
MbiI	CCGCTC	1 cut(s)	818
MboI	GATC	5 cut(s)	1024, 1173, 1462, 1956, 2058
MfeI	CAATTG	1 cut(s)	1323
MhlI	GDGCHC	3 cut(s)	114, 873, 1087
MlsI	TGGCCA	1 cut(s)	792
MluI	ACGCGT	1 cut(s)	834
MluNI	TGGCCA	1 cut(s)	792
MlyI	GAGTC	2 cut(s)	77, 2032
MmeI	TCCRAC	3 cut(s)	912, 1331, 1440
Mox20I	TGGCCA	1 cut(s)	792
Mph1103I	ATGCAT	3 cut(s)	1494, 1647, 2447
MroI	TCCGGA	1 cut(s)	1653
MroXI	GAANNNNTTC	2 cut(s)	2120, 2230
MscI	TGGCCA	1 cut(s)	792
MslI	CAYNNNNRTG	3 cut(s)	1245, 1497, 1932
Msp20I	TGGCCA	1 cut(s)	792
MspCI	CTTAAG	1 cut(s)	2125
MspI	CCGG	2 cut(s)	30, 1654
MspR9I	CCNGG	2 cut(s)	1052, 1202
MunI	CAATTG	1 cut(s)	1323
MvaI	CCWGG	2 cut(s)	1052, 1202
MvnI	CGCG	1 cut(s)	836
MwoI	GCNNNNNNNGC	5 cut(s)	664, 700, 769, 838, 1237
NdeII	GATC	5 cut(s)	1024, 1173, 1462, 1956, 2058
NlaIV	GGNNCC	2 cut(s)	527, 1652
NmeAIII	GCCGAG	2 cut(s)	22, 1095
NmuCI	GTSAC	6 cut(s)	290, 991, 1204, 1939, 2044, 2368
NsiI	ATGCAT	3 cut(s)	1494, 1647, 2447
NspI	RCATGY	3 cut(s)	1492, 1912, 2256
NspV	TTCGAA	1 cut(s)	25
PaeR7I	CTCGAG	1 cut(s)	872
PciI	ACATGT	1 cut(s)	1908
PdmI	GAANNNNTTC	2 cut(s)	2120, 2230
PfeI	GAWTC	5 cut(s)	209, 430, 2290, 2335, 2341
PflFI	GACNNNGTC	1 cut(s)	1202
PflMI	CCANNNNNTGG	1 cut(s)	799
PkrI	GCNGC	2 cut(s)	40, 1888
PleI	GAGTC	2 cut(s)	76, 2032
PpsI	GAGTC	2 cut(s)	76, 2032
PpuMI	RGGWCCY	1 cut(s)	948
PscI	ACATGT	1 cut(s)	1908
PshAI	GACNNNNGTC	1 cut(s)	2042
PshBI	ATTAAT	1 cut(s)	1737
PsiI	TTATAA	2 cut(s)	999, 1212
Psp124BI	GAGCTC	1 cut(s)	114
Psp1406I	AACGTT	1 cut(s)	1789
Psp5II	RGGWCCY	1 cut(s)	948
Psp6I	CCWGG	2 cut(s)	1050, 1200
PspGI	CCWGG	2 cut(s)	1050, 1200
PspN4I	GGNNCC	2 cut(s)	527, 1652
PspPI	GGNCC	3 cut(s)	525, 754, 948
PspPPI	RGGWCCY	1 cut(s)	948
PspXI	VCTCGAGB	1 cut(s)	872
PsyI	GACNNNGTC	1 cut(s)	1202
RsaI	GTAC	2 cut(s)	683, 1329
RsaNI	GTAC	2 cut(s)	682, 1328
RseI	CAYNNNNRTG	3 cut(s)	1245, 1497, 1932
SacI	GAGCTC	1 cut(s)	114
SalI	GTCGAC	1 cut(s)	1446
SatI	GCNGC	2 cut(s)	39, 1887
Sau3AI	GATC	5 cut(s)	1024, 1173, 1462, 1956, 2058
Sau96I	GGNCC	3 cut(s)	525, 754, 948
SchI	GAGTC	2 cut(s)	77, 2032
ScrFI	CCNGG	2 cut(s)	1052, 1202
SduI	GDGCHC	3 cut(s)	114, 873, 1087
SexAI	ACCWGGT	1 cut(s)	1200
Sfr274I	CTCGAG	1 cut(s)	872
SfuI	TTCGAA	1 cut(s)	25
SinI	GGWCC	2 cut(s)	525, 948
SlaI	CTCGAG	1 cut(s)	872
SmiMI	CAYNNNNRTG	3 cut(s)	1245, 1497, 1932
SmlI	CTYRAG	5 cut(s)	148, 695, 872, 1390, 2125
SmoI	CTYRAG	5 cut(s)	148, 695, 872, 1390, 2125
SsiI	CCGC	6 cut(s)	260, 345, 658, 763, 818, 866
SspMI	CTAG	2 cut(s)	732, 2387
SstI	GAGCTC	1 cut(s)	114
StyD4I	CCNGG	2 cut(s)	1050, 1200
StyI	CCWWGG	2 cut(s)	724, 951
TaaI	ACNGT	7 cut(s)	296, 1004, 1111, 1519, 1690, 1849, 2427
TaiI	ACGT	1 cut(s)	1792
TaqI	TCGA	7 cut(s)	25, 250, 814, 873, 1447, 2190, 2320
TatI	WGTACW	2 cut(s)	681, 1327
TfiI	GAWTC	5 cut(s)	209, 430, 2290, 2335, 2341
TscAI	CASTG	3 cut(s)	659, 1429, 1966
TseFI	GTSAC	6 cut(s)	290, 991, 1204, 1939, 2044, 2368
TseI	GCWGC	2 cut(s)	38, 1886
Tsp45I	GTSAC	6 cut(s)	290, 991, 1204, 1939, 2044, 2368
TspDTI	ATGAA	9 cut(s)	480, 627, 977, 1077, 1323, 1601, 1783, 1844, 2024
TspRI	CASTG	3 cut(s)	659, 1429, 1966
Tth111I	GACNNNGTC	1 cut(s)	1202
Van91I	CCANNNNNTGG	1 cut(s)	799
Vha464I	CTTAAG	1 cut(s)	2125
VpaK11BI	GGWCC	2 cut(s)	525, 948
VspI	ATTAAT	1 cut(s)	1737
XagI	CCTNNNNNAGG	2 cut(s)	955, 1127
XapI	RAATTY	2 cut(s)	400, 1265
XceI	RCATGY	3 cut(s)	1492, 1912, 2256
XhoI	CTCGAG	1 cut(s)	872
XmiI	GTMKAC	1 cut(s)	1447
XmnI	GAANNNNTTC	2 cut(s)	2120, 2230
XspI	CTAG	2 cut(s)	732, 2387
Zsp2I	ATGCAT	3 cut(s)	1494, 1647, 2447

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.