Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0002968

Physical Location & Seq

Genomic Coordinates Reverse (-)

14070694 .. 14075326

Gene Structure 4633 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000022885

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2373 bp

ATGGTATTGGGAGGAACAAGGAGAGCAGGAGCACAAAGGACCTGGCATTGGACTCTGGGGTCAAACTCCCAACCAGAGACCATGGCTTCCGGTGGGGATAACATCTCTGTTACTAAAGGCGTTGTTTCTAGGGTTGAACCTACACAATATGTTCACGGTGCAACCCAGCTTATGGCAATACAGATCGATGCAGCAATAAATCCCGGGAATAGTGGTAGCCCAGCAATCATGGGAAATAAAGTTGCTGGTGTTGCTTTCCAAAACCTTTCTGGTGCAGAGAATATTGGTTATATAATTCCTGTTCCTGTAATAAAGCATTTTATTGCTGGAGTTGAAGAAAGAGGTAAATATTCTGGAGATGGAGATGAAGATGAATATAGAGACCCTTTGGTGAGAGAGGTTTGTAGGTTAATTGAGCTTAGAACGGCTTGGACCCCAAAGTTTGAAGGTGAATTAAGGCACTTGTTACGAAGCTTGAAGCCAAAGCAGGTTTGTGCTGTTTTGAAGTCTCAGGCTGATGAAAGAGTTGCTTTGGAGGTTTTCTACTGGGCTGATAGGCAGTGGAGGTATAAGCATGACCCGATTGTGTACTATGCAATGCTTGAGGTGCTTAGTAAGACCAAGTTGTGCCAAGGTGCTAGGAGGATTCTTAGGTTAATGGCCCGTAGGCGGATTGAGCTTCGTTCTGAAGCTTTTGGTCATGTGATGGTGTCATATAGTCGAGCGGGCAAGTTAAGACATGCATTGCGTGTGTTGACTTTGATGCAGAAAGCGGGGCTTGAGCTTGATTTGTCAATGTGTAATACTGCAATTTATGTTTTGGTGAAAGGTAATAAGTTAGAGAAGGCGTTGAGGACCTTAGAAAGGATGAAGCTTGTTGGGATTGCACCAAATGTTGTCACTTATAACTGTTTGATAAAGGGGTATTGTGATTTGCATCGTGTAGAAGATGCAATCCAGGTGATTGATGAAATGCCGATGAAGGGGTGTGCTCCTGATAAAGTTAGTTATTATACTGTCATGAATTTCCTTTGTAAGGAGAAGAGGGTCAAAGAAGTGAGGGACTTGATGGAGAAGATGATCAAGGATGGTGGGTTGTTACCAGACCAGGTCACTTATAGTAATCTTGTCCATGTGCTTTGTAAGCACGGCTATGGTGATGAGGCACTTGAATTTTTAAGGGAAGCCGAAGAGAGGGGATTTCGGTTTGATAAGGTTGGGTATAGTGCAATTGTACATTCATTTTGTAAGGATGGTAGGATTGACATGGCAAAAGAAATTGTTAGTGAAATGTTCTCAAAAGGTTGCACACCAGATGTTGTTACATACACTACTGTTCTGAATGGGTATTGTCGACTTGGGAAGGTGGATCAAGCTAAAAAGATGCTTCAGCACATGTATAAGCATGGGTGCAAGCCAAATACTGTATCATATACGGCCATAATAAATGGGCTTTGTCGTGGTGGGAGCTCATTGGAGGCAAGGGAGATGATGAACATGAGTGAGGAAGAATGGTGGACACCAAATGCCATCACTTACAGTGTTCTGATGCATGGGTTCCGGAGGGAAGGGAAATTGGTTGAGGCTTGTGATATTGTCAAACAGATGATTAAAAAGGGACTTTTTCCTACCCCAGTTGAAATTAATCTCTTAATTCAGTCTCTTTGCCAAGAGGGGAAGATGGATGAGGCTAAACATTTCATGGAGGAGTGTTTAAACAAAGGTTGTGCTGTTAATGTTGTCAATTTTACTACTGTTATTCATGGTTATTGTCAAAAAGATGACTTGGAAGCTGCTCTGTCATTGCTTGATGACATGTATCTCAGCAACAAACACGCAGATGCAGTAACTTATACAGCAGCGATCAATGCATTGGGAAAGAAGGGTAGAATACAAGAGGCAACAGAACTTATGATGAAGATGCTGGGTAAGGGTATTGACCCGACTCCCGTCACATACAGGACGATCATCCACTGGTATTGTAAGATGAATAGGGTGGATGATTTGTTAAAATTATTGGAGAAGATGTTCTTGAGGCAACGTTGCAAAACAACATATAATCAAGTGATTGAGAAGCTTTGTAGTTTTGGGAACTTTGAGGATGCTGATAAACTATTGGGTAAGGTGTTGAGAACAGCTTCAAGAGTTGATGCTAAAACATGCCATGTTGTTATGGATGGTTATTTGAGAAAAGAGGTTCCTCTATTAGCATACAAAGTGGCTTGTCGAATGTTCAATCGGAATCTGATTCCTGATTTAAAGTTGTGTCAGAAGGTGACCAAGAGATTGATGCTAGAAGGATACTCCAAAGAGGCAGACAACCTTATGTTACGGACTTTGATATGGACCTCAGGTTGTAAGGGATCAGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016070 GO:0016787 GO:0016788 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

791

Amino Acids

89.33

Weight (kDa)

8.98

Isoelectric Point (pI)

30.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	206 - 291	2.9e-06	Fungal tetratrico peptide repeats
PPR_long	PF17177	232 - 395	3.2e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	251 - 309	4.6e-12	Pentatricopeptide repeat domain
PPR_2	PF13041	266 - 311	3.1e-11	PPR repeat family
PPR_1	PF12854	294 - 325	1.1e-11	PPR repeat
PPR_2	PF13041	297 - 346	4.3e-16	PPR repeat family
PPR	PF01535	300 - 330	4.8e-07	PPR repeat
PPR_2	PF13041	332 - 382	9.9e-10	PPR repeat family
PPR_1	PF12854	365 - 395	9.4e-08	PPR repeat
PPR_2	PF13041	368 - 417	1.5e-08	PPR repeat family
PPR	PF01535	371 - 401	9.7e-06	PPR repeat
PPR_long	PF17177	393 - 522	8e-10	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	393 - 443	1.8e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	399 - 431	3.4e-06	PPR repeat
PPR_2	PF13041	405 - 452	2.5e-14	PPR repeat family
PPR_1	PF12854	434 - 466	2.2e-13	PPR repeat
PPR_3	PF13812	434 - 483	3.4e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	438 - 487	2e-19	PPR repeat family
PPR	PF01535	441 - 471	2.1e-07	PPR repeat
PPR_1	PF12854	469 - 499	8.7e-08	PPR repeat
TPR_24	PF23276	509 - 603	3.3e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	574 - 606	4.2e-08	PPR repeat
PPR_2	PF13041	579 - 625	1.3e-10	PPR repeat family
PPR_3	PF13812	601 - 662	7.8e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	614 - 658	4.1e-10	PPR repeat family
PPR	PF01535	616 - 646	8.7e-06	PPR repeat
PPR_1	PF12854	644 - 676	9.5e-08	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002968)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30290 AT1G30290
fragaria_vesca	FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050
malus_domestica	MD10G1258300.v1.1
prunus_persica	Prupe.4G085500_v2.0.a1 Prupe.4G085500_v2.0.a1
pyrus_communis	pycom05g26000 pycom10g21460
rosa_chinensis	RchiOBHm_Chr3g0490541 RchiOBHm_Chr3g0490551 RchiOBHm_Chr5g0014911
rosa_laevigata	RLG00000022885 RLG00000032150
rosa_multiflora	Rmu_sc0003862.1_g000007 Rmu_sc0013610.1_g000002
rosa_roxburghii	Rroxscaffold_1G00061660 Rroxscaffold_6G00393420
rosa_rugosa	Rorug03G0243900 Rorug05G0021400
rosa_samantha	Rh3AG293300 Rh3AG293400 Rh3BG329800 Rh3CG327300 Rh3CG327400 Rh3DG326700 Rh5AG117100 Rh5BG114100 Rh5CG125600 Rh5DG113500
rosa_wichuraiana	Rw3G025970 Rw5G010080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	906
AasI	GACNNNNNNGTC	1 cut(s)	58
Acc36I	ACCTGC	1 cut(s)	478
AccB7I	CCANNNNNTGG	1 cut(s)	172
AccBSI	CCGCTC	1 cut(s)	725
AccI	GTMKAC	1 cut(s)	1354
AccIII	TCCGGA	1 cut(s)	1560
AciI	CCGC	3 cut(s)	670, 725, 773
AclI	AACGTT	1 cut(s)	2041
AclWI	GGATC	2 cut(s)	1377, 2371
AcoI	YGGCCR	1 cut(s)	1437
AcsI	RAATTY	2 cut(s)	1024, 1172
AcuI	CTGAAG	2 cut(s)	708, 1373
AfaI	GTAC	2 cut(s)	590, 1236
AfiI	CCNNNNNNNGG	7 cut(s)	48, 172, 669, 864, 983, 1036, 1194
AflIII	ACRYGT	2 cut(s)	1395, 1815
AgsI	TTSAA	9 cut(s)	137, 335, 446, 478, 505, 1172, 1640, 2142, 2236
AjnI	CCWGG	3 cut(s)	41, 957, 1107
AloI	GAACNNNNNNTCC	2 cut(s)	2012, 2044
Alw21I	GWGCWC	3 cut(s)	34, 994, 1472
Alw26I	GTCTC	4 cut(s)	71, 375, 513, 1665
AlwI	GGATC	2 cut(s)	1377, 2371
Ama87I	CYCGRG	1 cut(s)	203
Aor13HI	TCCGGA	1 cut(s)	1560
AoxI	GGCC	2 cut(s)	660, 1437
ApeKI	GCWGC	3 cut(s)	191, 1793, 1859
ApoI	RAATTY	2 cut(s)	1024, 1172
AseI	ATTAAT	1 cut(s)	1644
Asp700I	GAANNNNTTC	2 cut(s)	2027, 2137
AspS9I	GGNCC	5 cut(s)	39, 432, 661, 855, 2346
AsuC2I	CCSGG	2 cut(s)	204, 205
AsuHPI	GGTGA	6 cut(s)	403, 461, 835, 973, 1169, 2287
AvaI	CYCGRG	1 cut(s)	203
AvaII	GGWCC	4 cut(s)	39, 432, 855, 2346
AxyI	CCTNAGG	1 cut(s)	2350
BanII	GRGCYC	1 cut(s)	1472
Bbv12I	GWGCWC	3 cut(s)	34, 994, 1472
BbvI	GCAGC	3 cut(s)	203, 1780, 1871
BccI	CCATC	8 cut(s)	353, 700, 1063, 1082, 1247, 1538, 1675, 2171
BceAI	ACGGC	3 cut(s)	441, 1165, 1452
BciT130I	CCWGG	3 cut(s)	43, 959, 1109
BciVI	GTATCC	1 cut(s)	2294
BclI	TGATCA	1 cut(s)	1080
BcnI	CCSGG	2 cut(s)	204, 205
BcoDI	GTCTC	4 cut(s)	71, 375, 513, 1665
BfaI	CTAG	3 cut(s)	129, 639, 2294
BfuAI	ACCTGC	1 cut(s)	478
BfuI	GTATCC	1 cut(s)	2294
BisI	GCNGC	3 cut(s)	192, 1794, 1860
BlsI	GCNGC	3 cut(s)	193, 1795, 1861
Bme1390I	CCNGG	5 cut(s)	43, 204, 205, 959, 1109
Bme18I	GGWCC	4 cut(s)	39, 432, 855, 2346
BmeT110I	CYCGRG	1 cut(s)	203
BmgT120I	GGNCC	5 cut(s)	39, 432, 661, 855, 2346
BmiI	GGNNCC	3 cut(s)	434, 1559, 2199
BmrFI	CCNGG	5 cut(s)	43, 204, 205, 959, 1109
BmrI	ACTGGG	2 cut(s)	556, 1628
BmuI	ACTGGG	2 cut(s)	556, 1628
BoxI	GACNNNNGTC	1 cut(s)	1949
BpmI	CTGGAG	2 cut(s)	348, 375
BpuEI	CTTGAG	3 cut(s)	623, 800, 2053
BpuMI	CCSGG	2 cut(s)	204, 205
Bsa29I	ATCGAT	1 cut(s)	186
BsaBI	GATNNNNATC	1 cut(s)	936
BsaI	GGTCTC	2 cut(s)	71, 375
BsaJI	CCNNGG	3 cut(s)	81, 203, 631
BsaWI	WCCGGW	2 cut(s)	89, 1560
BsaXI	ACNNNNNCTCC	2 cut(s)	2012, 2042
Bsc4I	CCNNNNNNNGG	7 cut(s)	48, 172, 669, 864, 983, 1036, 1194
Bse1I	ACTGG	3 cut(s)	551, 1634, 1979
Bse21I	CCTNAGG	1 cut(s)	2350
Bse3DI	GCAATG	3 cut(s)	603, 743, 1802
Bse8I	GATNNNNATC	1 cut(s)	936
BseAI	TCCGGA	1 cut(s)	1560
BseBI	CCWGG	3 cut(s)	43, 959, 1109
BseCI	ATCGAT	1 cut(s)	186
BseDI	CCNNGG	3 cut(s)	81, 203, 631
BseGI	GGATG	8 cut(s)	873, 1093, 1258, 1690, 1968, 2005, 2107, 2182
BseJI	GATNNNNATC	1 cut(s)	936
BseLI	CCNNNNNNNGG	7 cut(s)	48, 172, 669, 864, 983, 1036, 1194
BseMI	GCAATG	3 cut(s)	603, 743, 1802
BseMII	CTCAG	3 cut(s)	524, 1837, 2364
BseNI	ACTGG	3 cut(s)	551, 1634, 1979
BseRI	GAGGAG	1 cut(s)	1721
BseXI	GCAGC	3 cut(s)	203, 1780, 1871
BseYI	CCCAGC	3 cut(s)	165, 220, 1924
BsgI	GTGCAG	1 cut(s)	294
BshFI	GGCC	2 cut(s)	662, 1439
BshVI	ATCGAT	1 cut(s)	186
BsiHKAI	GWGCWC	3 cut(s)	34, 994, 1472
BsiHKCI	CYCGRG	1 cut(s)	203
BsiSI	CCGG	3 cut(s)	90, 204, 1561
BslFI	GGGAC	2 cut(s)	1076, 1632
BslI	CCNNNNNNNGG	7 cut(s)	48, 172, 669, 864, 983, 1036, 1194
BsmAI	GTCTC	4 cut(s)	71, 375, 513, 1665
BsmFI	GGGAC	2 cut(s)	1076, 1632
BsnI	GGCC	2 cut(s)	662, 1439
Bso31I	GGTCTC	2 cut(s)	71, 375
BsoBI	CYCGRG	1 cut(s)	203
Bsp1286I	GDGCHC	3 cut(s)	34, 994, 1472
Bsp13I	TCCGGA	1 cut(s)	1560
Bsp1407I	TGTACA	1 cut(s)	1234
Bsp143I	GATC	6 cut(s)	183, 1080, 1369, 1863, 1965, 2363
Bsp19I	CCATGG	1 cut(s)	81
BspACI	CCGC	3 cut(s)	670, 725, 773
BspANI	GGCC	2 cut(s)	662, 1439
BspCNI	CTCAG	3 cut(s)	523, 1836, 2363
BspDI	ATCGAT	1 cut(s)	186
BspEI	TCCGGA	1 cut(s)	1560
BspHI	TCATGA	1 cut(s)	1020
BspLI	GGNNCC	3 cut(s)	434, 1559, 2199
BspMI	ACCTGC	1 cut(s)	478
BspPI	GGATC	2 cut(s)	1377, 2371
BspTNI	GGTCTC	2 cut(s)	71, 375
BsrBI	CCGCTC	1 cut(s)	725
BsrDI	GCAATG	3 cut(s)	603, 743, 1802
BsrGI	TGTACA	1 cut(s)	1234
BsrI	ACTGG	3 cut(s)	551, 1634, 1979
BssECI	CCNNGG	3 cut(s)	81, 203, 631
BssMI	GATC	6 cut(s)	183, 1080, 1369, 1863, 1965, 2363
BssT1I	CCWWGG	2 cut(s)	81, 631
Bst2UI	CCWGG	3 cut(s)	43, 959, 1109
Bst4CI	ACNGT	7 cut(s)	158, 911, 1018, 1336, 1426, 1541, 1756
Bst6I	CTCTTC	2 cut(s)	1037, 1185
BstAUI	TGTACA	1 cut(s)	1234
BstC8I	GCNNGC	2 cut(s)	727, 1415
BstDEI	CTNAG	7 cut(s)	419, 510, 611, 650, 859, 1823, 2350
BstDSI	CCRYGG	1 cut(s)	81
BstEII	GGTNACC	1 cut(s)	2275
BstENI	CCTNNNNNAGG	2 cut(s)	862, 1034
BstF5I	GGATG	8 cut(s)	873, 1093, 1258, 1690, 1968, 2005, 2107, 2182
BstKTI	GATC	6 cut(s)	186, 1083, 1372, 1866, 1968, 2366
BstMAI	GTCTC	4 cut(s)	71, 375, 513, 1665
BstMBI	GATC	6 cut(s)	183, 1080, 1369, 1863, 1965, 2363
BstMWI	GCNNNNNNNGC	5 cut(s)	251, 607, 676, 1144, 1868
BstNI	CCWGG	3 cut(s)	43, 959, 1109
BstNSI	RCATGY	4 cut(s)	743, 1399, 1819, 2163
BstPAI	GACNNNNGTC	1 cut(s)	1949
BstPI	GGTNACC	1 cut(s)	2275
BstSCI	CCNGG	5 cut(s)	41, 202, 203, 957, 1107
BstV1I	GCAGC	3 cut(s)	203, 1780, 1871
Bsu15I	ATCGAT	1 cut(s)	186
Bsu36I	CCTNAGG	1 cut(s)	2350
BsuI	GTATCC	1 cut(s)	2294
BsuRI	GGCC	2 cut(s)	662, 1439
BsuTUI	ATCGAT	1 cut(s)	186
BtgI	CCRYGG	1 cut(s)	81
BtsCI	GGATG	8 cut(s)	873, 1093, 1258, 1690, 1968, 2005, 2107, 2182
BtsI	GCAGTG	1 cut(s)	566
BtsIMutI	CAGTG	3 cut(s)	566, 1546, 1972
BveI	ACCTGC	1 cut(s)	478
Cac8I	GCNNGC	2 cut(s)	727, 1415
CciI	TCATGA	1 cut(s)	1020
Cfr13I	GGNCC	5 cut(s)	39, 432, 661, 855, 2346
Cfr9I	CCCGGG	1 cut(s)	203
ClaI	ATCGAT	1 cut(s)	186
CsiI	ACCWGGT	1 cut(s)	1107
Csp6I	GTAC	2 cut(s)	589, 1235
CspCI	CAANNNNNGTGG	4 cut(s)	1072, 1107, 1961, 1996
CviQI	GTAC	2 cut(s)	589, 1235
DdeI	CTNAG	7 cut(s)	419, 510, 611, 650, 859, 1823, 2350
DpnI	GATC	6 cut(s)	185, 1082, 1371, 1865, 1967, 2365
DpnII	GATC	6 cut(s)	183, 1080, 1369, 1863, 1965, 2363
DraI	TTTAAA	2 cut(s)	1716, 2259
DrdI	GACNNNNNNGTC	1 cut(s)	58
DseDI	GACNNNNNNGTC	1 cut(s)	58
EaeI	YGGCCR	1 cut(s)	1437
Eam1104I	CTCTTC	2 cut(s)	1037, 1185
EarI	CTCTTC	2 cut(s)	1037, 1185
EciI	GGCGGA	1 cut(s)	685
Ecl136II	GAGCTC	1 cut(s)	1470
Eco130I	CCWWGG	2 cut(s)	81, 631
Eco24I	GRGCYC	1 cut(s)	1472
Eco31I	GGTCTC	2 cut(s)	71, 375
Eco47I	GGWCC	4 cut(s)	39, 432, 855, 2346
Eco53kI	GAGCTC	1 cut(s)	1470
Eco57I	CTGAAG	2 cut(s)	708, 1373
Eco81I	CCTNAGG	1 cut(s)	2350
Eco88I	CYCGRG	1 cut(s)	203
Eco91I	GGTNACC	1 cut(s)	2275
EcoICRI	GAGCTC	1 cut(s)	1470
EcoNI	CCTNNNNNAGG	2 cut(s)	862, 1034
EcoO109I	RGGNCCY	2 cut(s)	39, 855
EcoO65I	GGTNACC	1 cut(s)	2275
EcoRII	CCWGG	3 cut(s)	41, 957, 1107
EcoT14I	CCWWGG	2 cut(s)	81, 631
EcoT22I	ATGCAT	3 cut(s)	745, 1554, 1873
EcoT38I	GRGCYC	1 cut(s)	1472
ErhI	CCWWGG	2 cut(s)	81, 631
FalI	AAGNNNNNCTT	6 cut(s)	514, 546, 762, 794, 2015, 2047
FaqI	GGGAC	2 cut(s)	1076, 1632
FauI	CCCGC	2 cut(s)	718, 766
FbaI	TGATCA	1 cut(s)	1080
FblI	GTMKAC	1 cut(s)	1354
Fnu4HI	GCNGC	3 cut(s)	192, 1794, 1860
FokI	GGATG	8 cut(s)	880, 1100, 1265, 1697, 1955, 2012, 2114, 2189
FriOI	GRGCYC	1 cut(s)	1472
Fsp4HI	GCNGC	3 cut(s)	192, 1794, 1860
FspBI	CTAG	3 cut(s)	129, 639, 2294
GluI	GCNGC	3 cut(s)	192, 1794, 1860
GsaI	CCCAGC	3 cut(s)	169, 224, 1928
GsuI	CTGGAG	2 cut(s)	348, 375
HaeIII	GGCC	2 cut(s)	662, 1439
HapII	CCGG	3 cut(s)	90, 204, 1561
HincII	GTYRAC	2 cut(s)	756, 1355
HindII	GTYRAC	2 cut(s)	756, 1355
HindIII	AAGCTT	4 cut(s)	472, 690, 872, 2075
HinfI	GANTC	5 cut(s)	52, 646, 1945, 2242, 2248
HpaII	CCGG	3 cut(s)	90, 204, 1561
HphI	GGTGA	6 cut(s)	403, 461, 835, 973, 1169, 2287
Hpy166II	GTNNAC	5 cut(s)	154, 589, 756, 1355, 1518
Hpy188I	TCNGA	6 cut(s)	688, 1341, 1548, 2241, 2247, 2271
Hpy188III	TCNNGA	7 cut(s)	354, 995, 1021, 1561, 2032, 2142, 2252
Hpy8I	GTNNAC	5 cut(s)	154, 589, 756, 1355, 1518
HpyAV	CCTTC	8 cut(s)	440, 838, 976, 1357, 1562, 1876, 2266, 2291
HpyCH4III	ACNGT	7 cut(s)	158, 911, 1018, 1336, 1426, 1541, 1756
HpyCH4IV	ACGT	1 cut(s)	2041
HpyF10VI	GCNNNNNNNGC	5 cut(s)	251, 607, 676, 1144, 1868
HpyF3I	CTNAG	7 cut(s)	419, 510, 611, 650, 859, 1823, 2350
HpySE526I	ACGT	1 cut(s)	2041
Kpn2I	TCCGGA	1 cut(s)	1560
Ksp22I	TGATCA	1 cut(s)	1080
Kzo9I	GATC	6 cut(s)	183, 1080, 1369, 1863, 1965, 2363
LmnI	GCTCC	3 cut(s)	29, 997, 1467
Lsp1109I	GCAGC	3 cut(s)	203, 1780, 1871
MabI	ACCWGGT	1 cut(s)	1107
MaeI	CTAG	3 cut(s)	129, 639, 2294
MaeII	ACGT	1 cut(s)	2041
MalI	GATC	6 cut(s)	185, 1082, 1371, 1865, 1967, 2365
MbiI	CCGCTC	1 cut(s)	725
MboI	GATC	6 cut(s)	183, 1080, 1369, 1863, 1965, 2363
MfeI	CAATTG	1 cut(s)	1230
MhlI	GDGCHC	3 cut(s)	34, 994, 1472
MlyI	GAGTC	2 cut(s)	46, 1939
Mph1103I	ATGCAT	3 cut(s)	745, 1554, 1873
MroI	TCCGGA	1 cut(s)	1560
MroXI	GAANNNNTTC	2 cut(s)	2027, 2137
MslI	CAYNNNNRTG	2 cut(s)	1152, 1839
MspI	CCGG	3 cut(s)	90, 204, 1561
MspR9I	CCNGG	5 cut(s)	43, 204, 205, 959, 1109
MssI	GTTTAAAC	1 cut(s)	1716
MunI	CAATTG	1 cut(s)	1230
MvaI	CCWGG	3 cut(s)	43, 959, 1109
MwoI	GCNNNNNNNGC	5 cut(s)	251, 607, 676, 1144, 1868
NciI	CCSGG	2 cut(s)	204, 205
NcoI	CCATGG	1 cut(s)	81
NdeII	GATC	6 cut(s)	183, 1080, 1369, 1863, 1965, 2363
NlaIV	GGNNCC	3 cut(s)	434, 1559, 2199
NmuCI	GTSAC	4 cut(s)	898, 1111, 1951, 2275
NsiI	ATGCAT	3 cut(s)	745, 1554, 1873
NspI	RCATGY	4 cut(s)	743, 1399, 1819, 2163
PagI	TCATGA	1 cut(s)	1020
PciI	ACATGT	2 cut(s)	1395, 1815
PdmI	GAANNNNTTC	2 cut(s)	2027, 2137
PfeI	GAWTC	3 cut(s)	646, 2242, 2248
PflFI	GACNNNGTC	1 cut(s)	1109
PflMI	CCANNNNNTGG	1 cut(s)	172
PkrI	GCNGC	3 cut(s)	193, 1795, 1861
PleI	GAGTC	2 cut(s)	46, 1939
PmeI	GTTTAAAC	1 cut(s)	1716
PpsI	GAGTC	2 cut(s)	46, 1939
PpuMI	RGGWCCY	2 cut(s)	39, 855
PscI	ACATGT	2 cut(s)	1395, 1815
PshAI	GACNNNNGTC	1 cut(s)	1949
PshBI	ATTAAT	1 cut(s)	1644
PsiI	TTATAA	1 cut(s)	906
Psp124BI	GAGCTC	1 cut(s)	1472
Psp1406I	AACGTT	1 cut(s)	2041
Psp5II	RGGWCCY	2 cut(s)	39, 855
Psp6I	CCWGG	3 cut(s)	41, 957, 1107
PspEI	GGTNACC	1 cut(s)	2275
PspFI	CCCAGC	3 cut(s)	165, 220, 1924
PspGI	CCWGG	3 cut(s)	41, 957, 1107
PspN4I	GGNNCC	3 cut(s)	434, 1559, 2199
PspPI	GGNCC	5 cut(s)	39, 432, 661, 855, 2346
PspPPI	RGGWCCY	2 cut(s)	39, 855
PsyI	GACNNNGTC	1 cut(s)	1109
RsaI	GTAC	2 cut(s)	590, 1236
RsaNI	GTAC	2 cut(s)	589, 1235
RseI	CAYNNNNRTG	2 cut(s)	1152, 1839
SacI	GAGCTC	1 cut(s)	1472
SalI	GTCGAC	1 cut(s)	1353
SatI	GCNGC	3 cut(s)	192, 1794, 1860
Sau3AI	GATC	6 cut(s)	183, 1080, 1369, 1863, 1965, 2363
Sau96I	GGNCC	5 cut(s)	39, 432, 661, 855, 2346
SchI	GAGTC	2 cut(s)	46, 1939
ScrFI	CCNGG	5 cut(s)	43, 204, 205, 959, 1109
SduI	GDGCHC	3 cut(s)	34, 994, 1472
SexAI	ACCWGGT	1 cut(s)	1107
SinI	GGWCC	4 cut(s)	39, 432, 855, 2346
SmaI	CCCGGG	1 cut(s)	205
SmiMI	CAYNNNNRTG	2 cut(s)	1152, 1839
SmlI	CTYRAG	3 cut(s)	602, 779, 2032
SmoI	CTYRAG	3 cut(s)	602, 779, 2032
SsiI	CCGC	3 cut(s)	670, 725, 773
SspI	AATATT	2 cut(s)	283, 350
SspMI	CTAG	3 cut(s)	129, 639, 2294
SstI	GAGCTC	1 cut(s)	1472
StyD4I	CCNGG	5 cut(s)	41, 202, 203, 957, 1107
StyI	CCWWGG	2 cut(s)	81, 631
TaaI	ACNGT	7 cut(s)	158, 911, 1018, 1336, 1426, 1541, 1756
TaiI	ACGT	1 cut(s)	2044
TaqI	TCGA	4 cut(s)	186, 721, 1354, 2227
TatI	WGTACW	2 cut(s)	588, 1234
TfiI	GAWTC	3 cut(s)	646, 2242, 2248
TscAI	CASTG	3 cut(s)	566, 1546, 1979
TseFI	GTSAC	4 cut(s)	898, 1111, 1951, 2275
TseI	GCWGC	3 cut(s)	191, 1793, 1859
Tsp45I	GTSAC	4 cut(s)	898, 1111, 1951, 2275
TspGWI	ACGGA	1 cut(s)	2347
TspMI	CCCGGG	1 cut(s)	203
TspRI	CASTG	3 cut(s)	566, 1546, 1979
Tth111I	GACNNNGTC	1 cut(s)	1109
Van91I	CCANNNNNTGG	1 cut(s)	172
VpaK11BI	GGWCC	4 cut(s)	39, 432, 855, 2346
VspI	ATTAAT	1 cut(s)	1644
XagI	CCTNNNNNAGG	2 cut(s)	862, 1034
XapI	RAATTY	2 cut(s)	1024, 1172
XceI	RCATGY	4 cut(s)	743, 1399, 1819, 2163
XcmI	CCANNNNNNNNNTGG	1 cut(s)	266
XmaI	CCCGGG	1 cut(s)	203
XmiI	GTMKAC	1 cut(s)	1354
XmnI	GAANNNNTTC	2 cut(s)	2027, 2137
XspI	CTAG	3 cut(s)	129, 639, 2294
Zsp2I	ATGCAT	3 cut(s)	745, 1554, 1873

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.