RchiOBHm_Chr5g0014911

Pentatricopeptide repeat-containing protein

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
Physical Location & Seq
Reverse (-)
10307807 .. 10311147
3341 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29534

Sequence Viewer

Length: 1731 bp
ATGGCCCGTAGGCGGATTGAGATTCGTTCTGAAGCTTTTTGCCATGTGATGGTGTCATATAGTCGAGCGGGCAAGTTGAGGCACGCGTTGCGTGTGTTGACTTTGATGCAGAAGGCGGGGCTTGAGCTTGATTTGTCAATGTGCAATACTGCAATTTATGTTTTGGTTAAGGGTAATAAGTTGGAGAAGGCGTTGAGGACCTTGGAAAGGATGAAGCTTGTTGGGATTGCACCAAATGTAGTCACTTATAACTGTTTGATAAAGGGGTATTGTGATCTGCATCGTGTCGAAGATGCAATCCAGGTGATTGATGAAATGCCGAGGAAGGGGTGTGCTCCTGATAAAGTTAGTTACTATACTGTGATGGATTTCCTTTGTAAGGAGAAGAGGGTCAAAGAAGTGAGGGACTTGATGGAGAAGATGATCAAGGATGGTGGGTTGTTACCAGACCAGGTCACGTATAATAATCTTGTCCATGTGCTTTGTAAGCATGGCTATGGTGATGAGGCACTTGAATTTTTAAGGGAAGCCGAAGAGAAGGGATTTCGGTTTGATAAGGTTGGGTATAGTGCAATTGTACATTCATTTTGTAAGGATGGTCGGACTGACATGGCAAAAGAAATTGTTAGTGAAATGTTCTCAAAAGGTTGCATCCCAGATGTTGTAACATACACTGCTGTTCTGAATGGGTATTGTCGACTTGGGAAGTTGGATCAAGCTAAAAAGATGCTTCAGCACATGCATAAGCATGGTTGCAAGCCAAATACTGTATCATATACGGCCTTATTAAATGGGCTTTGTCATGGTGGGAACTCATTGGAGGCAAGAGAGATGATGAACACGAGTGAGGAAGAATGGTGGACACCAAATGCCATCACTTATAGTGTTCTGATGCATGGGTTCCGGAGGGAAGGGAAATTGGTTGAGGCTTGTGATACTGTCAAAGAGATGATTAAAAAGGGATTTTTCCCTACCCCAGTTGAAATTAATCTCTTAATTCAGTCTCTTTGCCAAGAGTGGAAGATGGATGAGGCTAAGCGTTTCATGGAGGAGTGTCTAAATAAAGGTTGTGCTGTTAATGTTGTCAATTTTACTACTGTTATTCATGGATATTGTCAAAAAGATGACTTGGAAGCTGCTCTATCGTTGCTTGATGACATGTATCTCAGCAATAAACACCCAGATGCAGTAACTTATACAGCAATGATCAGTGCATTGGGAAAGAAAGGTAGAATACAAGACGCAACTGAACTTATGATGAAGATGCTGAATAAGGGTATTGACCCGACTCCCGTCACATACAGGACGATCATCCATTGGTATTGTAAGATGAGTAGGGTGGATGATTTGTTGAAATTATTGGAGCAGATGTTCTTAAGGCAAAACTGCAAAACAGCATATAATCAAGTGATTGAGAAGCTTTGTAGTTTTGGGAACTTTGAGGACGCTGATAAACTATTGGGTAAGGTGTTGAGAACAGCTTCAAGAGTTGATGCTAAAACATGCCATGTTGTTATGGATGGTTATTTGAGAAAAGGGATTCCTCTGTCAGCATACAAAGTGGCTTGTCGAATGTTCAATCGGAATCTGATTCCTGATTTCAAGTTGTGTGAGAAGGTGACGAAGAGACTGATGCTAGAAGGAAACTCCAAAGAGGCAGACAACCTTATGTTACGGTTTGTTGATCGTGGATGCATATCAAACCAACATCAGGAACATCTGGAAAGCTAA

Protein Analysis

576

Amino Acids

65.81

Weight (kDa)

8.63

Isoelectric Point (pI)

29.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 13 - 176 3.4e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 32 - 90 3.1e-12 Pentatricopeptide repeat domain
PPR_2 PF13041 46 - 92 1.7e-11 PPR repeat family
PPR_1 PF12854 75 - 106 7.6e-12 PPR repeat
PPR_2 PF13041 78 - 123 2e-15 PPR repeat family
PPR PF01535 81 - 111 7.3e-08 PPR repeat
PPR_1 PF12854 146 - 176 1.1e-08 PPR repeat
PPR_2 PF13041 149 - 198 3.7e-10 PPR repeat family
PPR PF01535 152 - 182 9.7e-07 PPR repeat
PPR_long PF17177 171 - 304 1.2e-11 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 174 - 229 6.8e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 180 - 212 5.4e-06 PPR repeat
PPR_2 PF13041 186 - 233 3.9e-13 PPR repeat family
PPR_3 PF13812 208 - 264 2.9e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 215 - 247 6.7e-12 PPR repeat
PPR_2 PF13041 219 - 268 3.3e-19 PPR repeat family
PPR PF01535 222 - 252 5.3e-08 PPR repeat
PPR_1 PF12854 250 - 280 2e-06 PPR repeat
PPR_2 PF13041 289 - 325 8.1e-06 PPR repeat family
TPR_24 PF23276 290 - 384 6.3e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 355 - 387 2.9e-08 PPR repeat
PPR_2 PF13041 360 - 408 1.2e-13 PPR repeat family
PPR_3 PF13812 382 - 443 1.7e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 393 - 422 3.6e-07 PPR repeat
PPR_2 PF13041 394 - 443 1.9e-14 PPR repeat family
PPR PF01535 397 - 427 4e-07 PPR repeat
PPR_1 PF12854 425 - 457 4.9e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 249
AccB7I CCANNNNNTGG 1 cut(s) 49
AccBSI CCGCTC 1 cut(s) 68
AccI GTMKAC 1 cut(s) 697
AccII CGCG 1 cut(s) 86
AccIII TCCGGA 1 cut(s) 903
AciI CCGC 3 cut(s) 13, 68, 116
AclWI GGATC 1 cut(s) 720
AcsI RAATTY 1 cut(s) 515
AcuI CTGAAG 2 cut(s) 51, 716
AfaI GTAC 1 cut(s) 579
AfiI CCNNNNNNNGG 6 cut(s) 12, 49, 207, 326, 379, 1711
AflII CTTAAG 1 cut(s) 1375
AflIII ACRYGT 2 cut(s) 84, 1158
AgsI TTSAA 6 cut(s) 515, 983, 1354, 1485, 1579, 1603
AjnI CCWGG 2 cut(s) 300, 450
AloI GAACNNNNNNTCC 2 cut(s) 1355, 1387
AluBI AGCT 8 cut(s) 35, 127, 217, 719, 1136, 1420, 1481, 1728
AluI AGCT 8 cut(s) 35, 127, 217, 719, 1136, 1420, 1481, 1728
Alw21I GWGCWC 1 cut(s) 337
Alw26I GTCTC 2 cut(s) 1008, 1621
AlwI GGATC 1 cut(s) 720
Aor13HI TCCGGA 1 cut(s) 903
AoxI GGCC 2 cut(s) 3, 780
ApeKI GCWGC 1 cut(s) 1136
ApoI RAATTY 1 cut(s) 515
AseI ATTAAT 1 cut(s) 987
Asp700I GAANNNNTTC 1 cut(s) 1480
AspS9I GGNCC 2 cut(s) 4, 198
AsuHPI GGTGA 3 cut(s) 316, 512, 1630
AvaII GGWCC 1 cut(s) 198
BauI CACGAG 1 cut(s) 841
Bbv12I GWGCWC 1 cut(s) 337
BbvI GCAGC 1 cut(s) 1123
BccI CCATC 8 cut(s) 43, 358, 406, 425, 590, 881, 1018, 1514
BceAI ACGGC 1 cut(s) 795
BciT130I CCWGG 2 cut(s) 302, 452
BclI TGATCA 2 cut(s) 423, 1206
BcoDI GTCTC 2 cut(s) 1008, 1621
BfaI CTAG 1 cut(s) 1637
BfrI CTTAAG 1 cut(s) 1375
BisI GCNGC 1 cut(s) 1137
BlpI GCTNAGC 1 cut(s) 1035
BlsI GCNGC 1 cut(s) 1138
Bme1390I CCNGG 2 cut(s) 302, 452
Bme18I GGWCC 1 cut(s) 198
BmgT120I GGNCC 2 cut(s) 4, 198
BmiI GGNNCC 1 cut(s) 902
BmrFI CCNGG 2 cut(s) 302, 452
BmrI ACTGGG 1 cut(s) 971
BmuI ACTGGG 1 cut(s) 971
BoxI GACNNNNGTC 1 cut(s) 1292
Bpu1102I GCTNAGC 1 cut(s) 1035
BpuEI CTTGAG 1 cut(s) 143
BsaAI YACGTR 1 cut(s) 459
BsaBI GATNNNNATC 2 cut(s) 279, 1696
BsaJI CCNNGG 2 cut(s) 201, 320
BsaWI WCCGGW 1 cut(s) 903
BsaXI ACNNNNNCTCC 4 cut(s) 176, 206, 1355, 1385
Bsc4I CCNNNNNNNGG 6 cut(s) 12, 49, 207, 326, 379, 1711
Bse1I ACTGG 1 cut(s) 977
Bse3DI GCAATG 1 cut(s) 1209
Bse8I GATNNNNATC 2 cut(s) 279, 1696
BseAI TCCGGA 1 cut(s) 903
BseBI CCWGG 2 cut(s) 302, 452
BseDI CCNNGG 2 cut(s) 201, 320
BseGI GGATG 9 cut(s) 216, 436, 601, 651, 1033, 1311, 1348, 1525, 1697
BseJI GATNNNNATC 2 cut(s) 279, 1696
BseLI CCNNNNNNNGG 6 cut(s) 12, 49, 207, 326, 379, 1711
BseMI GCAATG 1 cut(s) 1209
BseMII CTCAG 1 cut(s) 1180
BseNI ACTGG 1 cut(s) 977
BseRI GAGGAG 1 cut(s) 1064
BseXI GCAGC 1 cut(s) 1123
Bsh1236I CGCG 1 cut(s) 86
BshFI GGCC 2 cut(s) 5, 782
BsiHKAI GWGCWC 1 cut(s) 337
BsiSI CCGG 1 cut(s) 904
BslFI GGGAC 1 cut(s) 419
BslI CCNNNNNNNGG 6 cut(s) 12, 49, 207, 326, 379, 1711
BsmAI GTCTC 2 cut(s) 1008, 1621
BsmFI GGGAC 1 cut(s) 419
BsnI GGCC 2 cut(s) 5, 782
Bsp1286I GDGCHC 1 cut(s) 337
Bsp13I TCCGGA 1 cut(s) 903
Bsp1407I TGTACA 1 cut(s) 577
Bsp143I GATC 6 cut(s) 274, 423, 712, 1206, 1308, 1684
Bsp1720I GCTNAGC 1 cut(s) 1035
BspACI CCGC 3 cut(s) 13, 68, 116
BspANI GGCC 2 cut(s) 5, 782
BspCNI CTCAG 1 cut(s) 1179
BspEI TCCGGA 1 cut(s) 903
BspFNI CGCG 1 cut(s) 86
BspLI GGNNCC 1 cut(s) 902
BspPI GGATC 1 cut(s) 720
BspTI CTTAAG 1 cut(s) 1375
BsrBI CCGCTC 1 cut(s) 68
BsrDI GCAATG 1 cut(s) 1209
BsrGI TGTACA 1 cut(s) 577
BsrI ACTGG 1 cut(s) 977
BssECI CCNNGG 2 cut(s) 201, 320
BssMI GATC 6 cut(s) 274, 423, 712, 1206, 1308, 1684
BssSI CACGAG 1 cut(s) 841
BssT1I CCWWGG 1 cut(s) 201
Bst2BI CACGAG 1 cut(s) 841
Bst2UI CCWGG 2 cut(s) 302, 452
Bst4CI ACNGT 6 cut(s) 254, 361, 769, 940, 1099, 1677
Bst6I CTCTTC 3 cut(s) 380, 528, 1619
BstAFI CTTAAG 1 cut(s) 1375
BstAPI GCANNNNNTGC 1 cut(s) 88
BstAUI TGTACA 1 cut(s) 577
BstBAI YACGTR 1 cut(s) 459
BstC8I GCNNGC 3 cut(s) 70, 84, 758
BstDEI CTNAG 2 cut(s) 1035, 1166
BstENI CCTNNNNNAGG 2 cut(s) 205, 377
BstF5I GGATG 9 cut(s) 216, 436, 601, 651, 1033, 1311, 1348, 1525, 1697
BstFNI CGCG 1 cut(s) 86
BstKTI GATC 6 cut(s) 277, 426, 715, 1209, 1311, 1687
BstMAI GTCTC 2 cut(s) 1008, 1621
BstMBI GATC 6 cut(s) 274, 423, 712, 1206, 1308, 1684
BstMWI GCNNNNNNNGC 2 cut(s) 88, 487
BstNI CCWGG 2 cut(s) 302, 452
BstNSI RCATGY 3 cut(s) 742, 1162, 1506
BstPAI GACNNNNGTC 1 cut(s) 1292
BstSCI CCNGG 2 cut(s) 300, 450
BstUI CGCG 1 cut(s) 86
BstV1I GCAGC 1 cut(s) 1123
BsuRI GGCC 2 cut(s) 5, 782
BtsCI GGATG 9 cut(s) 216, 436, 601, 651, 1033, 1311, 1348, 1525, 1697
BtsI GCAGTG 1 cut(s) 672
BtsIMutI CAGTG 2 cut(s) 672, 1216
Cac8I GCNNGC 3 cut(s) 70, 84, 758
Cfr13I GGNCC 2 cut(s) 4, 198
CseI GACGC 2 cut(s) 1250, 1454
CsiI ACCWGGT 1 cut(s) 450
Csp6I GTAC 1 cut(s) 578
CspCI CAANNNNNGTGG 2 cut(s) 415, 450
CviQI GTAC 1 cut(s) 578
DdeI CTNAG 2 cut(s) 1035, 1166
DpnI GATC 6 cut(s) 276, 425, 714, 1208, 1310, 1686
DpnII GATC 6 cut(s) 274, 423, 712, 1206, 1308, 1684
Eam1104I CTCTTC 3 cut(s) 380, 528, 1619
EarI CTCTTC 3 cut(s) 380, 528, 1619
EciI GGCGGA 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 198
Eco57I CTGAAG 2 cut(s) 51, 716
EcoNI CCTNNNNNAGG 2 cut(s) 205, 377
EcoO109I RGGNCCY 1 cut(s) 198
EcoRII CCWGG 2 cut(s) 300, 450
EcoT14I CCWWGG 1 cut(s) 201
EcoT22I ATGCAT 3 cut(s) 744, 897, 1697
ErhI CCWWGG 1 cut(s) 201
FaqI GGGAC 1 cut(s) 419
FauI CCCGC 2 cut(s) 61, 109
FbaI TGATCA 2 cut(s) 423, 1206
FblI GTMKAC 1 cut(s) 697
Fnu4HI GCNGC 1 cut(s) 1137
FokI GGATG 9 cut(s) 223, 443, 608, 638, 1040, 1298, 1355, 1532, 1704
Fsp4HI GCNGC 1 cut(s) 1137
FspBI CTAG 1 cut(s) 1637
GluI GCNGC 1 cut(s) 1137
HaeIII GGCC 2 cut(s) 5, 782
HapII CCGG 1 cut(s) 904
HgaI GACGC 2 cut(s) 1250, 1454
HincII GTYRAC 2 cut(s) 99, 698
HindII GTYRAC 2 cut(s) 99, 698
HindIII AAGCTT 3 cut(s) 33, 215, 1418
HinfI GANTC 5 cut(s) 22, 1288, 1540, 1585, 1591
HpaII CCGG 1 cut(s) 904
HphI GGTGA 3 cut(s) 316, 512, 1630
Hpy166II GTNNAC 3 cut(s) 99, 698, 861
Hpy188I TCNGA 6 cut(s) 31, 603, 684, 891, 1584, 1590
Hpy188III TCNNGA 6 cut(s) 338, 904, 1485, 1595, 1712, 1721
Hpy8I GTNNAC 3 cut(s) 99, 698, 861
HpyAV CCTTC 7 cut(s) 106, 181, 319, 532, 905, 1609, 1634
HpyCH4III ACNGT 6 cut(s) 254, 361, 769, 940, 1099, 1677
HpyCH4IV ACGT 1 cut(s) 458
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 487
HpyF3I CTNAG 2 cut(s) 1035, 1166
HpySE526I ACGT 1 cut(s) 458
Kpn2I TCCGGA 1 cut(s) 903
Ksp22I TGATCA 2 cut(s) 423, 1206
Kzo9I GATC 6 cut(s) 274, 423, 712, 1206, 1308, 1684
LmnI GCTCC 2 cut(s) 340, 1363
Lsp1109I GCAGC 1 cut(s) 1123
MabI ACCWGGT 1 cut(s) 450
MaeI CTAG 1 cut(s) 1637
MaeII ACGT 1 cut(s) 458
MaeIII GTNAC 9 cut(s) 241, 350, 441, 454, 664, 1189, 1294, 1618, 1671
MalI GATC 6 cut(s) 276, 425, 714, 1208, 1310, 1686
MbiI CCGCTC 1 cut(s) 68
MboI GATC 6 cut(s) 274, 423, 712, 1206, 1308, 1684
MboII GAAGA 8 cut(s) 302, 397, 430, 545, 863, 1033, 1273, 1636
MfeI CAATTG 1 cut(s) 573
MhlI GDGCHC 1 cut(s) 337
MluCI AATT 9 cut(s) 153, 515, 573, 621, 917, 984, 996, 1087, 1355
MluI ACGCGT 1 cut(s) 84
MlyI GAGTC 1 cut(s) 1282
MmeI TCCRAC 3 cut(s) 162, 581, 690
Mph1103I ATGCAT 3 cut(s) 744, 897, 1697
MroI TCCGGA 1 cut(s) 903
MroXI GAANNNNTTC 1 cut(s) 1480
MseI TTAA 8 cut(s) 168, 521, 788, 954, 987, 995, 1077, 1376
MslI CAYNNNNRTG 3 cut(s) 495, 747, 1182
MspCI CTTAAG 1 cut(s) 1375
MspI CCGG 1 cut(s) 904
MspR9I CCNGG 2 cut(s) 302, 452
MunI CAATTG 1 cut(s) 573
MvaI CCWGG 2 cut(s) 302, 452
MvnI CGCG 1 cut(s) 86
MwoI GCNNNNNNNGC 2 cut(s) 88, 487
NdeII GATC 6 cut(s) 274, 423, 712, 1206, 1308, 1684
NlaIV GGNNCC 1 cut(s) 902
NmeAIII GCCGAG 1 cut(s) 345
NmuCI GTSAC 4 cut(s) 241, 454, 1294, 1618
NsiI ATGCAT 3 cut(s) 744, 897, 1697
NspI RCATGY 3 cut(s) 742, 1162, 1506
PciI ACATGT 1 cut(s) 1158
PdmI GAANNNNTTC 1 cut(s) 1480
PfeI GAWTC 4 cut(s) 22, 1540, 1585, 1591
PflFI GACNNNGTC 1 cut(s) 452
PflMI CCANNNNNTGG 1 cut(s) 49
PkrI GCNGC 1 cut(s) 1138
PleI GAGTC 1 cut(s) 1282
PpsI GAGTC 1 cut(s) 1282
Ppu21I YACGTR 1 cut(s) 459
PpuMI RGGWCCY 1 cut(s) 198
PscI ACATGT 1 cut(s) 1158
PshAI GACNNNNGTC 1 cut(s) 1292
PshBI ATTAAT 1 cut(s) 987
PsiI TTATAA 1 cut(s) 249
Psp5II RGGWCCY 1 cut(s) 198
Psp6I CCWGG 2 cut(s) 300, 450
PspGI CCWGG 2 cut(s) 300, 450
PspN4I GGNNCC 1 cut(s) 902
PspPI GGNCC 2 cut(s) 4, 198
PspPPI RGGWCCY 1 cut(s) 198
PsyI GACNNNGTC 1 cut(s) 452
RsaI GTAC 1 cut(s) 579
RsaNI GTAC 1 cut(s) 578
RseI CAYNNNNRTG 3 cut(s) 495, 747, 1182
SalI GTCGAC 1 cut(s) 696
SaqAI TTAA 8 cut(s) 168, 521, 788, 954, 987, 995, 1077, 1376
SatI GCNGC 1 cut(s) 1137
Sau3AI GATC 6 cut(s) 274, 423, 712, 1206, 1308, 1684
Sau96I GGNCC 2 cut(s) 4, 198
SchI GAGTC 1 cut(s) 1282
ScrFI CCNGG 2 cut(s) 302, 452
SduI GDGCHC 1 cut(s) 337
SexAI ACCWGGT 1 cut(s) 450
SinI GGWCC 1 cut(s) 198
SmiMI CAYNNNNRTG 3 cut(s) 495, 747, 1182
SmlI CTYRAG 2 cut(s) 122, 1375
SmoI CTYRAG 2 cut(s) 122, 1375
Sse9I AATT 9 cut(s) 153, 515, 573, 621, 917, 984, 996, 1087, 1355
SsiI CCGC 3 cut(s) 13, 68, 116
SspMI CTAG 1 cut(s) 1637
StyD4I CCNGG 2 cut(s) 300, 450
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 6 cut(s) 254, 361, 769, 940, 1099, 1677
TaiI ACGT 1 cut(s) 461
TaqI TCGA 4 cut(s) 64, 288, 697, 1570
TasI AATT 9 cut(s) 153, 515, 573, 621, 917, 984, 996, 1087, 1355
TatI WGTACW 1 cut(s) 577
TfiI GAWTC 4 cut(s) 22, 1540, 1585, 1591
Tru1I TTAA 8 cut(s) 168, 521, 788, 954, 987, 995, 1077, 1376
Tru9I TTAA 8 cut(s) 168, 521, 788, 954, 987, 995, 1077, 1376
TscAI CASTG 2 cut(s) 679, 1216
TseFI GTSAC 4 cut(s) 241, 454, 1294, 1618
TseI GCWGC 1 cut(s) 1136
Tsp45I GTSAC 4 cut(s) 241, 454, 1294, 1618
TspDTI ATGAA 7 cut(s) 227, 327, 573, 851, 1033, 1094, 1274
TspRI CASTG 2 cut(s) 679, 1216
Tth111I GACNNNGTC 1 cut(s) 452
Van91I CCANNNNNTGG 1 cut(s) 49
Vha464I CTTAAG 1 cut(s) 1375
VpaK11BI GGWCC 1 cut(s) 198
VspI ATTAAT 1 cut(s) 987
XagI CCTNNNNNAGG 2 cut(s) 205, 377
XapI RAATTY 1 cut(s) 515
XceI RCATGY 3 cut(s) 742, 1162, 1506
XmiI GTMKAC 1 cut(s) 697
XmnI GAANNNNTTC 1 cut(s) 1480
XspI CTAG 1 cut(s) 1637
Zsp2I ATGCAT 3 cut(s) 744, 897, 1697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.