Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3B

Orthogroup ID

OG0002968

Physical Location & Seq

Genomic Coordinates Forward (+)

34465144 .. 34469297

Gene Structure 4154 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG329800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2490 bp

ATGTTGGTTACGTCCGCCCGTACACTCCTCAGGAGGCGGCTTTGCTCTATTGAGTATACGTCAAGGAATAGTTGTAGCAGGGCGGCAGCTAATGTTGAAGACAATGGTATTGGGAGGAACAAGGAGAGCAGGAGCACAAAGGACCTGGCATTGGACTCTGGGGTCAACTCCCAACCAGAGACCATGGCTTCCGGTGGGGATAACATCTCTGTTACTAAAGGCATTGTTTCTAGGGTTGAACCTACACAATATGTTCATGGAGCAACCCAGCTTATGGCAATACAGATCGATGCAGCAATAAATCCCGGGAATAGTGGTGGCCCAGCAATCATGGGAAATAAAGTTGCTGGTGTTGCTTTCCAAAACCTTTCTGGTGCAGAGAATATCGGTTATATAATTCCTGTTCCTGTAATAAAGCAGTTTATTGCTGGAGTTGAAGAAAGAGGTAAATATTATGGAGATGGAGATGGAGATGAATATAGAGACCCTTTGGTGAGAGAGGTTTGTAGGTTAATTGAGCTTAGAACAGCTTGGACCCCAAAGTTTGAAGGTGAATTAAGGCACTTGTTACGAAGCTTGAAGCCAAACCAGGTTTGTGCTGTTTTGAAGTCTCAGGCTGATGAAAGAGTTGCTTTGGAGATTTTCTACTGGGCTGATAGGCAGTGGAGGTATAAGCATGACCCGATTGTGTACTATGCAATGCTTGAGGTGCTTAGTAAGACCAAGTTGTGCCAAGGTGCTAGGAGGATTCTTAGGTTAATGGCCCGTAGGCGGATTGAGCTTCGTTCTGAAGCTTTTGGTCATGTGATGGTGTCATATAGTCGAGCGGGCAAGTTAAGACATGCGTTGCGTGTGTTGACTTTGATGCAGAAAGCGGGGCTTGAGCTTGATTTGTCAATGTGTAATACTGCAATTTATGTTTTGGTGAAAGGTAAAAAGTTAGAGAAGGCATTGAGGACCTTGGAAAGGATGAAGCTGGTTGGGATTGCACCAAATGTTGTCACTTATAACTGTTTGATAAAGGGGTATTGTGATTTGCATCGTGTAGAAGATGCAATCCAGGTGATTGATGAAATGCCGATGAAGGGGTGTGCTCCTGATAAAGTTAGTTATTATACTGTGATGAATTTCCTTTGTAAGGAGAAGAGGGTCAAAGAAGTGAGGGACTTGATGGAGAAGATGATCAAGGATGGTGGGTTGTTACCAGACCAGGTCACTTATAGTAATCTTGTCCATGTGCTTTGTAAGCACGGCTATGGTGATGAGGCACTTGAATTTTTAAGGGAAGCCGAAGAGAGGGGATTTCGGTTTGATAAGATTGGGTATAGTGCAATTGTACATTCATTTTGTAAGGATGGTAGGATTGACATGGCAAAAGAAATTGTTAGTGAAATGTTCTCAAAAGGTTGCACACCAGATGTTGTTACATACACTGCTGTTCTGAATGGGTATTGTCGACTTGGGAAGGTGGATCAAGCTAAAAAGATGCTTCAGCACATGTATAAGCATGGGTGCAAGCCAAATACTGTATCATATACGGCCATAATAAATGGGCTTTGTCGTGGTGGGAACTCATTGGAGGCAAGGGAGATGATGAACATGAGTGAGGAAGAATGGTGGACACCAAATGCCATCACTTACAGTGTTCTGATGCATGGGTTCCGGAGGGAAGGAAAATTGGTTGAGTCTTGTGATATTGTCAAACAGATGATTAAAAAGGGACTTTTTCCTACCCCAGTTGAAATTAATCTCTTAATTCAGTCTCTTTGCCAAGAGGGGACGATGGATGAGGCTAAACATTTCATGGAGGAGTGTTTAAACAAAGGTTGTGCTGTTAATGTTGTCAATTTTACTACTGTTGTTCATGGTTATTGTCAAAAAGATGACTTGGAAGTTGCTCTATCATTGCTTGATGACATGTATCTCAGCAACAAACACGCAGATGCAGTAACTTATACAGCAGTGATCAATGCATTGGGAAAGAAGGGTAGAATACAAAAGGCAACAGAACTTATGATGAAGATGCTGGGTAAGGGTATTGACCCGACTCCCGTCACATACAGGACGATCATCCACTGGTATTGTAAGATGAATAGGGTGGATGATTTGTTGAAATTATTGGAGAAGATGTTCTTGAGGCAACGTTGCAAAACAACATATAATCAAGTGATTGAGAAGCTTTGTAGTTTTGGGAACTTTGAGGATGCTGATAAACTATTGGGTAAGGTGTTGAGAACAGCTTCAAGAGTTGATGCTAAAACATGCCATGTTGTTATGGATGGTTATTTGAGAAAAGGGGTTCCTCTGTTAGCATACAAAGTGGCTTGTCGAATGTTCAATCGAAATCTGATTCCAGATTTAAAGTTGTGTCAGAAGGTGACCAAGAGATTGATGCTAGAAGGAAACTCCAAAGAGGCAGACAACCTTATGTTACGGTTTGTTGAGCGTGGATGCATATCAAACCAACTTCAGGAACATCTGGAAAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016070 GO:0016787 GO:0016788 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

829

Amino Acids

93.71

Weight (kDa)

8.98

Isoelectric Point (pI)

32.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trypsin	PF00089	71 - 142	9e-06	Trypsin
TPR_24	PF23276	240 - 325	2.1e-06	Fungal tetratrico peptide repeats
PPR_long	PF17177	266 - 429	3.2e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	285 - 343	7.3e-13	Pentatricopeptide repeat domain
PPR_2	PF13041	300 - 345	5e-11	PPR repeat family
PPR_1	PF12854	328 - 359	1.2e-11	PPR repeat
PPR_2	PF13041	331 - 380	4.6e-16	PPR repeat family
PPR	PF01535	334 - 364	5.1e-07	PPR repeat
PPR_2	PF13041	366 - 416	1.1e-09	PPR repeat family
PPR_1	PF12854	399 - 429	9.9e-08	PPR repeat
PPR_2	PF13041	402 - 451	2.9e-08	PPR repeat family
PPR	PF01535	405 - 435	1e-05	PPR repeat
PPR_3	PF13812	427 - 483	4.4e-09	Pentatricopeptide repeat domain
PPR_long	PF17177	429 - 557	9.7e-10	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	434 - 465	7.9e-06	PPR repeat
PPR_2	PF13041	440 - 486	2.4e-13	PPR repeat family
PPR_1	PF12854	468 - 500	2.1e-12	PPR repeat
PPR_3	PF13812	471 - 517	8.1e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	472 - 521	5e-19	PPR repeat family
PPR	PF01535	475 - 505	3.9e-07	PPR repeat
PPR_1	PF12854	503 - 533	1.8e-08	PPR repeat
PPR_1	PF12854	608 - 640	2.6e-07	PPR repeat
PPR_2	PF13041	613 - 659	1.1e-09	PPR repeat family
PPR_3	PF13812	635 - 696	1.4e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	648 - 692	5.6e-11	PPR repeat family
PPR	PF01535	650 - 680	2.1e-06	PPR repeat
PPR_1	PF12854	678 - 710	1e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002968)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30290 AT1G30290
fragaria_vesca	FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050
malus_domestica	MD10G1258300.v1.1
prunus_persica	Prupe.4G085500_v2.0.a1 Prupe.4G085500_v2.0.a1
pyrus_communis	pycom05g26000 pycom10g21460
rosa_chinensis	RchiOBHm_Chr3g0490541 RchiOBHm_Chr3g0490551 RchiOBHm_Chr5g0014911
rosa_laevigata	RLG00000022885 RLG00000032150
rosa_multiflora	Rmu_sc0003862.1_g000007 Rmu_sc0013610.1_g000002
rosa_roxburghii	Rroxscaffold_1G00061660 Rroxscaffold_6G00393420
rosa_rugosa	Rorug03G0243900 Rorug05G0021400
rosa_samantha	Rh3AG293300 Rh3AG293400 Rh3BG329800 Rh3CG327300 Rh3CG327400 Rh3DG326700 Rh5AG117100 Rh5BG114100 Rh5CG125600 Rh5DG113500
rosa_wichuraiana	Rw3G025970 Rw5G010080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1008
AasI	GACNNNNNNGTC	1 cut(s)	161
AccB7I	CCANNNNNTGG	1 cut(s)	274
AccBSI	CCGCTC	1 cut(s)	827
AccI	GTMKAC	2 cut(s)	56, 1456
AccIII	TCCGGA	1 cut(s)	1662
AciI	CCGC	6 cut(s)	15, 37, 83, 772, 827, 875
AclI	AACGTT	1 cut(s)	2143
AclWI	GGATC	1 cut(s)	1479
AcoI	YGGCCR	1 cut(s)	1539
AcsI	RAATTY	2 cut(s)	1126, 1274
AcuI	CTGAAG	3 cut(s)	810, 1475, 2453
AfaI	GTAC	3 cut(s)	22, 692, 1338
AfiI	CCNNNNNNNGG	8 cut(s)	151, 274, 771, 966, 1085, 1138, 1296, 2470
AflIII	ACRYGT	2 cut(s)	1497, 1917
AjnI	CCWGG	4 cut(s)	144, 588, 1059, 1209
AloI	GAACNNNNNNTCC	2 cut(s)	2114, 2146
Alw21I	GWGCWC	2 cut(s)	137, 1096
Alw26I	GTCTC	4 cut(s)	173, 477, 615, 1767
AlwI	GGATC	1 cut(s)	1479
Ama87I	CYCGRG	1 cut(s)	305
Aor13HI	TCCGGA	1 cut(s)	1662
AoxI	GGCC	3 cut(s)	319, 762, 1539
ApeKI	GCWGC	2 cut(s)	86, 293
ApoI	RAATTY	2 cut(s)	1126, 1274
AseI	ATTAAT	1 cut(s)	1746
Asp700I	GAANNNNTTC	2 cut(s)	2129, 2239
AspS9I	GGNCC	5 cut(s)	142, 320, 534, 763, 957
AsuC2I	CCSGG	2 cut(s)	306, 307
AsuHPI	GGTGA	6 cut(s)	505, 563, 937, 1075, 1271, 2389
AvaI	CYCGRG	1 cut(s)	305
AvaII	GGWCC	3 cut(s)	142, 534, 957
AxyI	CCTNAGG	1 cut(s)	29
BbsI	GAAGAC	1 cut(s)	105
Bbv12I	GWGCWC	2 cut(s)	137, 1096
BbvI	GCAGC	2 cut(s)	98, 305
BccI	CCATC	9 cut(s)	455, 461, 802, 1165, 1184, 1349, 1640, 1777, 2273
BceAI	ACGGC	2 cut(s)	1267, 1554
BciT130I	CCWGG	4 cut(s)	146, 590, 1061, 1211
BclI	TGATCA	2 cut(s)	1182, 1965
BcnI	CCSGG	2 cut(s)	306, 307
BcoDI	GTCTC	4 cut(s)	173, 477, 615, 1767
BfaI	CTAG	3 cut(s)	231, 741, 2396
BisI	GCNGC	4 cut(s)	38, 84, 87, 294
BlsI	GCNGC	4 cut(s)	39, 85, 88, 295
Bme1390I	CCNGG	6 cut(s)	146, 306, 307, 590, 1061, 1211
Bme18I	GGWCC	3 cut(s)	142, 534, 957
BmeT110I	CYCGRG	1 cut(s)	305
BmgT120I	GGNCC	5 cut(s)	142, 320, 534, 763, 957
BmiI	GGNNCC	3 cut(s)	536, 1661, 2301
BmrFI	CCNGG	6 cut(s)	146, 306, 307, 590, 1061, 1211
BmrI	ACTGGG	2 cut(s)	658, 1730
BmuI	ACTGGG	2 cut(s)	658, 1730
BoxI	GACNNNNGTC	1 cut(s)	2051
BpiI	GAAGAC	1 cut(s)	105
BpmI	CTGGAG	1 cut(s)	450
BpuEI	CTTGAG	3 cut(s)	725, 902, 2155
BpuMI	CCSGG	2 cut(s)	306, 307
Bsa29I	ATCGAT	1 cut(s)	288
BsaBI	GATNNNNATC	2 cut(s)	1038, 2455
BsaI	GGTCTC	2 cut(s)	173, 477
BsaJI	CCNNGG	4 cut(s)	183, 305, 733, 960
BsaWI	WCCGGW	2 cut(s)	191, 1662
BsaXI	ACNNNNNCTCC	2 cut(s)	2114, 2144
Bsc4I	CCNNNNNNNGG	8 cut(s)	151, 274, 771, 966, 1085, 1138, 1296, 2470
Bse1I	ACTGG	3 cut(s)	653, 1736, 2081
Bse21I	CCTNAGG	1 cut(s)	29
Bse3DI	GCAATG	2 cut(s)	705, 1904
Bse8I	GATNNNNATC	2 cut(s)	1038, 2455
BseAI	TCCGGA	1 cut(s)	1662
BseBI	CCWGG	4 cut(s)	146, 590, 1061, 1211
BseCI	ATCGAT	1 cut(s)	288
BseDI	CCNNGG	4 cut(s)	183, 305, 733, 960
BseGI	GGATG	9 cut(s)	975, 1195, 1360, 1792, 2070, 2107, 2209, 2284, 2456
BseJI	GATNNNNATC	2 cut(s)	1038, 2455
BseLI	CCNNNNNNNGG	8 cut(s)	151, 274, 771, 966, 1085, 1138, 1296, 2470
BseMI	GCAATG	2 cut(s)	705, 1904
BseMII	CTCAG	3 cut(s)	43, 626, 1939
BseNI	ACTGG	3 cut(s)	653, 1736, 2081
BseRI	GAGGAG	2 cut(s)	17, 1823
BseXI	GCAGC	2 cut(s)	98, 305
BseYI	CCCAGC	3 cut(s)	267, 322, 2026
BsgI	GTGCAG	1 cut(s)	396
BshFI	GGCC	3 cut(s)	321, 764, 1541
BshVI	ATCGAT	1 cut(s)	288
BsiHKAI	GWGCWC	2 cut(s)	137, 1096
BsiHKCI	CYCGRG	1 cut(s)	305
BsiSI	CCGG	3 cut(s)	192, 306, 1663
BslFI	GGGAC	3 cut(s)	1178, 1734, 1792
BslI	CCNNNNNNNGG	8 cut(s)	151, 274, 771, 966, 1085, 1138, 1296, 2470
BsmAI	GTCTC	4 cut(s)	173, 477, 615, 1767
BsmFI	GGGAC	3 cut(s)	1178, 1734, 1792
BsnI	GGCC	3 cut(s)	321, 764, 1541
Bso31I	GGTCTC	2 cut(s)	173, 477
BsoBI	CYCGRG	1 cut(s)	305
Bsp1286I	GDGCHC	2 cut(s)	137, 1096
Bsp13I	TCCGGA	1 cut(s)	1662
Bsp1407I	TGTACA	1 cut(s)	1336
Bsp143I	GATC	5 cut(s)	285, 1182, 1471, 1965, 2067
Bsp19I	CCATGG	1 cut(s)	183
BspACI	CCGC	6 cut(s)	15, 37, 83, 772, 827, 875
BspANI	GGCC	3 cut(s)	321, 764, 1541
BspCNI	CTCAG	3 cut(s)	42, 625, 1938
BspDI	ATCGAT	1 cut(s)	288
BspEI	TCCGGA	1 cut(s)	1662
BspLI	GGNNCC	3 cut(s)	536, 1661, 2301
BspPI	GGATC	1 cut(s)	1479
BspTNI	GGTCTC	2 cut(s)	173, 477
BsrBI	CCGCTC	1 cut(s)	827
BsrDI	GCAATG	2 cut(s)	705, 1904
BsrGI	TGTACA	1 cut(s)	1336
BsrI	ACTGG	3 cut(s)	653, 1736, 2081
BssECI	CCNNGG	4 cut(s)	183, 305, 733, 960
BssMI	GATC	5 cut(s)	285, 1182, 1471, 1965, 2067
BssNAI	GTATAC	1 cut(s)	57
BssT1I	CCWWGG	3 cut(s)	183, 733, 960
Bst1107I	GTATAC	1 cut(s)	57
Bst2UI	CCWGG	4 cut(s)	146, 590, 1061, 1211
Bst4CI	ACNGT	6 cut(s)	1013, 1120, 1528, 1643, 1858, 2436
Bst6I	CTCTTC	2 cut(s)	1139, 1287
BstAUI	TGTACA	1 cut(s)	1336
BstC8I	GCNNGC	2 cut(s)	829, 1517
BstDEI	CTNAG	6 cut(s)	29, 521, 612, 713, 752, 1925
BstDSI	CCRYGG	1 cut(s)	183
BstEII	GGTNACC	1 cut(s)	2377
BstENI	CCTNNNNNAGG	2 cut(s)	964, 1136
BstF5I	GGATG	9 cut(s)	975, 1195, 1360, 1792, 2070, 2107, 2209, 2284, 2456
BstKTI	GATC	5 cut(s)	288, 1185, 1474, 1968, 2070
BstMAI	GTCTC	4 cut(s)	173, 477, 615, 1767
BstMBI	GATC	5 cut(s)	285, 1182, 1471, 1965, 2067
BstMWI	GCNNNNNNNGC	4 cut(s)	353, 709, 778, 1246
BstNI	CCWGG	4 cut(s)	146, 590, 1061, 1211
BstNSI	RCATGY	4 cut(s)	845, 1501, 1921, 2265
BstPAI	GACNNNNGTC	1 cut(s)	2051
BstPI	GGTNACC	1 cut(s)	2377
BstSCI	CCNGG	6 cut(s)	144, 304, 305, 588, 1059, 1209
BstV1I	GCAGC	2 cut(s)	98, 305
BstV2I	GAAGAC	1 cut(s)	105
BstZ17I	GTATAC	1 cut(s)	57
Bsu15I	ATCGAT	1 cut(s)	288
Bsu36I	CCTNAGG	1 cut(s)	29
BsuRI	GGCC	3 cut(s)	321, 764, 1541
BsuTUI	ATCGAT	1 cut(s)	288
BtgI	CCRYGG	1 cut(s)	183
BtsCI	GGATG	9 cut(s)	975, 1195, 1360, 1792, 2070, 2107, 2209, 2284, 2456
BtsI	GCAGTG	3 cut(s)	668, 1431, 1968
BtsIMutI	CAGTG	5 cut(s)	668, 1431, 1648, 1968, 2074
Cac8I	GCNNGC	2 cut(s)	829, 1517
Cfr13I	GGNCC	5 cut(s)	142, 320, 534, 763, 957
Cfr9I	CCCGGG	1 cut(s)	305
ClaI	ATCGAT	1 cut(s)	288
CsiI	ACCWGGT	2 cut(s)	588, 1209
Csp6I	GTAC	3 cut(s)	21, 691, 1337
CspCI	CAANNNNNGTGG	4 cut(s)	1174, 1209, 2063, 2098
CviQI	GTAC	3 cut(s)	21, 691, 1337
DdeI	CTNAG	6 cut(s)	29, 521, 612, 713, 752, 1925
DpnI	GATC	5 cut(s)	287, 1184, 1473, 1967, 2069
DpnII	GATC	5 cut(s)	285, 1182, 1471, 1965, 2067
DraI	TTTAAA	2 cut(s)	1818, 2361
DrdI	GACNNNNNNGTC	1 cut(s)	161
DseDI	GACNNNNNNGTC	1 cut(s)	161
EaeI	YGGCCR	1 cut(s)	1539
Eam1104I	CTCTTC	2 cut(s)	1139, 1287
EarI	CTCTTC	2 cut(s)	1139, 1287
EciI	GGCGGA	2 cut(s)	4, 787
Eco130I	CCWWGG	3 cut(s)	183, 733, 960
Eco31I	GGTCTC	2 cut(s)	173, 477
Eco47I	GGWCC	3 cut(s)	142, 534, 957
Eco57I	CTGAAG	3 cut(s)	810, 1475, 2453
Eco81I	CCTNAGG	1 cut(s)	29
Eco88I	CYCGRG	1 cut(s)	305
Eco91I	GGTNACC	1 cut(s)	2377
EcoNI	CCTNNNNNAGG	2 cut(s)	964, 1136
EcoO109I	RGGNCCY	2 cut(s)	142, 957
EcoO65I	GGTNACC	1 cut(s)	2377
EcoRII	CCWGG	4 cut(s)	144, 588, 1059, 1209
EcoT14I	CCWWGG	3 cut(s)	183, 733, 960
EcoT22I	ATGCAT	3 cut(s)	1656, 1975, 2456
ErhI	CCWWGG	3 cut(s)	183, 733, 960
FalI	AAGNNNNNCTT	6 cut(s)	616, 648, 864, 896, 2117, 2149
FaqI	GGGAC	3 cut(s)	1178, 1734, 1792
FauI	CCCGC	2 cut(s)	820, 868
FbaI	TGATCA	2 cut(s)	1182, 1965
FblI	GTMKAC	2 cut(s)	56, 1456
Fnu4HI	GCNGC	4 cut(s)	38, 84, 87, 294
FokI	GGATG	9 cut(s)	982, 1202, 1367, 1799, 2057, 2114, 2216, 2291, 2463
Fsp4HI	GCNGC	4 cut(s)	38, 84, 87, 294
FspBI	CTAG	3 cut(s)	231, 741, 2396
GluI	GCNGC	4 cut(s)	38, 84, 87, 294
GsaI	CCCAGC	3 cut(s)	271, 326, 2030
GsuI	CTGGAG	1 cut(s)	450
HaeIII	GGCC	3 cut(s)	321, 764, 1541
HapII	CCGG	3 cut(s)	192, 306, 1663
HincII	GTYRAC	3 cut(s)	166, 858, 1457
HindII	GTYRAC	3 cut(s)	166, 858, 1457
HindIII	AAGCTT	3 cut(s)	574, 792, 2177
HinfI	GANTC	5 cut(s)	155, 748, 1685, 2047, 2350
HpaII	CCGG	3 cut(s)	192, 306, 1663
HphI	GGTGA	6 cut(s)	505, 563, 937, 1075, 1271, 2389
Hpy166II	GTNNAC	7 cut(s)	23, 57, 166, 691, 858, 1457, 1620
Hpy188I	TCNGA	5 cut(s)	790, 1443, 1650, 2349, 2373
Hpy188III	TCNNGA	8 cut(s)	31, 1097, 1663, 2134, 2244, 2354, 2471, 2480
Hpy8I	GTNNAC	7 cut(s)	23, 57, 166, 691, 858, 1457, 1620
HpyAV	CCTTC	8 cut(s)	542, 940, 1078, 1459, 1664, 1978, 2368, 2393
HpyCH4III	ACNGT	6 cut(s)	1013, 1120, 1528, 1643, 1858, 2436
HpyCH4IV	ACGT	3 cut(s)	11, 59, 2143
HpyF10VI	GCNNNNNNNGC	4 cut(s)	353, 709, 778, 1246
HpyF3I	CTNAG	6 cut(s)	29, 521, 612, 713, 752, 1925
HpySE526I	ACGT	3 cut(s)	11, 59, 2143
Kpn2I	TCCGGA	1 cut(s)	1662
Ksp22I	TGATCA	2 cut(s)	1182, 1965
Kzo9I	GATC	5 cut(s)	285, 1182, 1471, 1965, 2067
LmnI	GCTCC	3 cut(s)	132, 260, 1099
Lsp1109I	GCAGC	2 cut(s)	98, 305
MabI	ACCWGGT	2 cut(s)	588, 1209
MaeI	CTAG	3 cut(s)	231, 741, 2396
MaeII	ACGT	3 cut(s)	11, 59, 2143
MalI	GATC	5 cut(s)	287, 1184, 1473, 1967, 2069
MbiI	CCGCTC	1 cut(s)	827
MboI	GATC	5 cut(s)	285, 1182, 1471, 1965, 2067
MboII	GAAGA	9 cut(s)	110, 449, 1061, 1156, 1189, 1304, 1622, 2032, 2137
MfeI	CAATTG	1 cut(s)	1332
MhlI	GDGCHC	2 cut(s)	137, 1096
MlyI	GAGTC	3 cut(s)	149, 1694, 2041
Mph1103I	ATGCAT	3 cut(s)	1656, 1975, 2456
MroI	TCCGGA	1 cut(s)	1662
MroXI	GAANNNNTTC	2 cut(s)	2129, 2239
MslI	CAYNNNNRTG	2 cut(s)	1254, 1941
MspI	CCGG	3 cut(s)	192, 306, 1663
MspR9I	CCNGG	6 cut(s)	146, 306, 307, 590, 1061, 1211
MssI	GTTTAAAC	1 cut(s)	1818
MunI	CAATTG	1 cut(s)	1332
MvaI	CCWGG	4 cut(s)	146, 590, 1061, 1211
MwoI	GCNNNNNNNGC	4 cut(s)	353, 709, 778, 1246
NciI	CCSGG	2 cut(s)	306, 307
NcoI	CCATGG	1 cut(s)	183
NdeII	GATC	5 cut(s)	285, 1182, 1471, 1965, 2067
NlaIV	GGNNCC	3 cut(s)	536, 1661, 2301
NmuCI	GTSAC	4 cut(s)	1000, 1213, 2053, 2377
NsiI	ATGCAT	3 cut(s)	1656, 1975, 2456
NspI	RCATGY	4 cut(s)	845, 1501, 1921, 2265
PciI	ACATGT	2 cut(s)	1497, 1917
PdmI	GAANNNNTTC	2 cut(s)	2129, 2239
PfeI	GAWTC	2 cut(s)	748, 2350
PflFI	GACNNNGTC	1 cut(s)	1211
PflMI	CCANNNNNTGG	1 cut(s)	274
PkrI	GCNGC	4 cut(s)	39, 85, 88, 295
PleI	GAGTC	3 cut(s)	149, 1693, 2041
PmeI	GTTTAAAC	1 cut(s)	1818
PpsI	GAGTC	3 cut(s)	149, 1693, 2041
PpuMI	RGGWCCY	2 cut(s)	142, 957
PscI	ACATGT	2 cut(s)	1497, 1917
PshAI	GACNNNNGTC	1 cut(s)	2051
PshBI	ATTAAT	1 cut(s)	1746
PsiI	TTATAA	1 cut(s)	1008
Psp1406I	AACGTT	1 cut(s)	2143
Psp5II	RGGWCCY	2 cut(s)	142, 957
Psp6I	CCWGG	4 cut(s)	144, 588, 1059, 1209
PspEI	GGTNACC	1 cut(s)	2377
PspFI	CCCAGC	3 cut(s)	267, 322, 2026
PspGI	CCWGG	4 cut(s)	144, 588, 1059, 1209
PspN4I	GGNNCC	3 cut(s)	536, 1661, 2301
PspPI	GGNCC	5 cut(s)	142, 320, 534, 763, 957
PspPPI	RGGWCCY	2 cut(s)	142, 957
PsyI	GACNNNGTC	1 cut(s)	1211
RsaI	GTAC	3 cut(s)	22, 692, 1338
RsaNI	GTAC	3 cut(s)	21, 691, 1337
RseI	CAYNNNNRTG	2 cut(s)	1254, 1941
SalI	GTCGAC	1 cut(s)	1455
SatI	GCNGC	4 cut(s)	38, 84, 87, 294
Sau3AI	GATC	5 cut(s)	285, 1182, 1471, 1965, 2067
Sau96I	GGNCC	5 cut(s)	142, 320, 534, 763, 957
SchI	GAGTC	3 cut(s)	149, 1694, 2041
ScrFI	CCNGG	6 cut(s)	146, 306, 307, 590, 1061, 1211
SduI	GDGCHC	2 cut(s)	137, 1096
SexAI	ACCWGGT	2 cut(s)	588, 1209
SinI	GGWCC	3 cut(s)	142, 534, 957
SmaI	CCCGGG	1 cut(s)	307
SmiMI	CAYNNNNRTG	2 cut(s)	1254, 1941
SmlI	CTYRAG	3 cut(s)	704, 881, 2134
SmoI	CTYRAG	3 cut(s)	704, 881, 2134
SsiI	CCGC	6 cut(s)	15, 37, 83, 772, 827, 875
SspI	AATATT	1 cut(s)	452
SspMI	CTAG	3 cut(s)	231, 741, 2396
StyD4I	CCNGG	6 cut(s)	144, 304, 305, 588, 1059, 1209
StyI	CCWWGG	3 cut(s)	183, 733, 960
TaaI	ACNGT	6 cut(s)	1013, 1120, 1528, 1643, 1858, 2436
TaiI	ACGT	3 cut(s)	14, 62, 2146
TaqI	TCGA	5 cut(s)	288, 823, 1456, 2329, 2341
TatI	WGTACW	2 cut(s)	690, 1336
TauI	GCSGC	2 cut(s)	40, 86
TfiI	GAWTC	2 cut(s)	748, 2350
TscAI	CASTG	5 cut(s)	668, 1438, 1648, 1968, 2081
TseFI	GTSAC	4 cut(s)	1000, 1213, 2053, 2377
TseI	GCWGC	2 cut(s)	86, 293
Tsp45I	GTSAC	4 cut(s)	1000, 1213, 2053, 2377
TspMI	CCCGGG	1 cut(s)	305
TspRI	CASTG	5 cut(s)	668, 1438, 1648, 1968, 2081
Tth111I	GACNNNGTC	1 cut(s)	1211
Van91I	CCANNNNNTGG	1 cut(s)	274
VpaK11BI	GGWCC	3 cut(s)	142, 534, 957
VspI	ATTAAT	1 cut(s)	1746
XagI	CCTNNNNNAGG	2 cut(s)	964, 1136
XapI	RAATTY	2 cut(s)	1126, 1274
XceI	RCATGY	4 cut(s)	845, 1501, 1921, 2265
XcmI	CCANNNNNNNNNTGG	1 cut(s)	368
XmaI	CCCGGG	1 cut(s)	305
XmiI	GTMKAC	2 cut(s)	56, 1456
XmnI	GAANNNNTTC	2 cut(s)	2129, 2239
XspI	CTAG	3 cut(s)	231, 741, 2396
Zsp2I	ATGCAT	3 cut(s)	1656, 1975, 2456

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.